Induction of DNA damage in the peripheral blood of zebrafish (Danio rerio) by an agricultural organophosphate pesticide,monocrotophos

The use of organophosphate pesticides (OPs) in agricultural practices in India has gained popularity due to their broad spectrum, low cost and high potency. Monocrotophos (an OP) which is a systemic and contact broad-spectrum cholinesterase inhibitor is being widely used in India. However, its use entails the damage to other non-target organisms such as fish. In the present study, zebrafish (Danio rerio) were exposed to various doses of monocrotophos (0.125, 0.625 and 1.25 μL/L) and genotoxicity studies were carried out employing the comet assay and micronucleus test in the peripheral blood of these fish after 24, 48 and 72 h of exposure. Significant DNA damage in the form of % tail DNA and micronuclei was observed in all the treated fish compared to the control. Both % tail DNA and micronuclei frequency were found to increase significantly as the concentration increased. DNA damage was also observed at all the time intervals for all treatments except in the 0.125 μL/L-treated group. A positive correlation was also observed between the comet assay and micronucleus test. The study thus suggests the use of zebrafish as an experimental model to study the genotoxic effects of agricultural pesticides using the comet assay and micronucleus test.

     

Virulence of different isolates of Aeromonas hydrophila in channel catfish

The virulence of nine Aeromonas hydrophila isolates from diseased fish, diseased Macrobrachium (freshwater shrimp) and from pond water was determined in channel catfish (Ictalurus punctatus) fingerlings. According to the lethal dose ? 50% end point (LD₅₀) of each isolate, the water organisms were significantly (P < 0.05) less virulent than the isolates from diseased fish. All isolates were biochemically similar.     

DNA damage as a biomarker for assessing the effects of suspended solids on the orange-spotted grouper, <Emphasis Type="Italic">Epinephelus coioides</Emphasis>

In Hong Kong, suspended solids (SS) introduced by dredging and mud disposal activities are a major cause of mass mortality in cage-cultured marine fish. We have used DNA damage in liver cells, as determined by the comet assay, to assess the impact of SS on the orange-spotted grouper Epinephelus coioides. Seabed sediments were collected from a heavily polluted site in Victoria Harbor and two less polluted sites in Port Shelter and Mirs Bay. Sediments from Victoria Harbor contained higher levels of copper (Cu) and polycyclic aromatic hydrocarbons (PAHs) than those from the other sites. In a 10-day experiment, SS from all three sites induced significant increase in comet tail length, but not in percentage (%) tail DNA. In a 20-day experiment, fish exposed to polluted SS from Victoria Harbor exhibited a significant increase in comet tail length after 5 days and % tail DNA after 10 days. After a 10-day recovery period, however, DNA damage was reduced as tail length and % tail DNA returned to control levels. These results suggest that DNA damage measured by the comet assay is a highly sensitive biomarker for assessing the genotoxic effects of SS to marine fish.     

The use of lidocaine-sodium bicarbonate as anaesthetic in fish

Lidocaine and lidocaine-sodium bicarbonate mixture were evaluated as anaesthetics for carp (Cyprinus carpio), catfish (Ictalurus punctatus) and tilapia (Tilapia mossambica = Oreochromis mossambicus). Although both induced anaesthesia, the mixture was more effective for induction and recovery, and was able to achieve the required time for the fish to be out of water. The responses varied for the three test species.     

Bioaccumulation of hexachlorocyclohexane,dichlorodiphenyltrichloroethane, and estradiol-17β in catfish and carp during the pre-monsoon season in India

This investigation was performed to monitor hexachlorocyclohexane isomers (HCHs), dichlorodiphenyltrichloroethane (DDT, and its metabolites, refered to as DDTs), plasma levels of estradiol-17β (E2), and the gonadosomatic index (GSI) between sampling sites of unpolluted ponds of Gujartal, Jaunpur (control site) and the polluted rivers Gomti (Jaunpur) and Ganga (Varanasi), which affect the reproductive physiology of some edible catfish and carp during the pre-monsoon season. HCHs and DDTs were measured by gas liquid chromatography (GLC) and hormones by radioimmunoassay (RIA). The results indicated that the level of HCHs and DDTs was very high in both the catfish and the carp captured from the polluted rivers compared with the fish captured from the control site. The GSI and E2 values were lower in both groups of fish when compared to the fish from the control site. The results also indicate that catfish showed greater bioaccumulation of HCHs and DDTs than carp, above the permissible limit, as compared to the fish from the control site. In conclusion, fish from the Gomti and Ganga rivers were highly polluted when compared with fish from the control site, as was evident from high levels of tissue bioaccumulation of HCHs and DDTs and decreased levels of plasma E2, inhibiting the reproductive physiology of these species at the receptor level. The levels exceeded the maximum residue limits (MRL) as recommended by Codex, hence it is suggested that the fish should be avoided for food purposes.     

The role of vitamin C in calcium uptake by fish

Calcium uptake in experimentally induced scorbutic snake heads, Channa (= Ophicephalus) punctatus, fed vitamin C-deficient diet for 210 days is reported, using ⁴⁵Ca as tracer. When compared with a parallel control on completely synthetic diet, the results show decreased absorption and utilization of calcium by gill, skin, muscle and bone of scorbutic fish from surrounding water. The physiological significance of these results is discussed, and its practical utility in aquaculture is indicated.     

Patterns of fish movement at a desert river confluence

Quantifying fish movements in river networks helps identify critical habitat needs and how they change with environmental conditions. Some of the challenges in tracking fish movements can be overcome with the use of passive integrated transponder (PIT) tagging and antennas. We used PIT technology to test predictions of movement behaviour for four fish species at a mainstem–tributary confluence zone in an arid‐land river system. Specifically, we focused on the McElmo Creek tributary confluence with the San Juan River in south‐western Utah, USA. We quantified variation in species occurrences at this confluence zone from May 2012 to December 2015 relative to temporal and environmental conditions. We considered occurrences among species relative to tagging origins (tributary versus mainstem), season and time of day. Generally, fishes tagged in the focal tributary were more likely to be detected compared to fish tagged in the mainstem river or other tributaries. Additionally, adults were most likely to be detected across multiple years compared to subadults. Based on a Random Forests model, the best performing environmental variables for predicting seasonal detections included mainstem discharge during run‐off season (razorback sucker Xyrauchen texanus), tributary discharge during monsoon season (Colorado pikeminnow Ptychocheilus lucius) and mainstem water temperature (flannelmouth sucker Catostomus latipinnis and channel catfish Ictalurus punctatus). The variable responses by endemic and introduced fishes indicate tributary habitats provide several key functions within a fish community including spawning, rearing, foraging and refuge.     

Effects of simazine treatment on channel catfish and bluegill production in ponds

Application of 13.4 kg/ha of simazine to the bottom of channel catfish (Ictalurus punctatus) ponds before flooding resulted in an extended period of low dissolved oxygen, a 19% reduction (P < 0.01) in channel catfish yield, and poorer feed conversion by fish when compared to control ponds. A single application of 1.5 mg/l of simazine to the water of fertilized bluegill (Lepomis macrochirus) ponds also decreased dissolved oxygen. The 11% reduction in bluegill yield was not statistically significant (P > 0.05). Simazine treatment caused an initial reduction in macrophyte coverage in bluegill ponds, but macrophyte coverage increased as simazine concentrations in the pond water declined.     

Revue des pratiques d'elevage de poissons en cages dans les eaux continentalesA review of fish cage culture as practised in inland waters

The rearing of fish in cages could greatly contribute to increasing fish production in existing inland waters. Although it presents numerous advantages, its limitations and possible inconveniences must be borne in mind. Well-developed technology is available mainly from the Democratic Republic of Germany, The Netherlands, Japan, the U.S.A., the U.S.S.R. and the Lower Mekong region. Research is currently pursued in numerous other countries, where the use of floating cages in fish culture is becoming increasingly diversified.The most popular fish species used in intensive cultural systems are Cyprinus carpio, Ictalurus punctatus and Salmo gairdneri. In the semi-intensive Asian systems, Barbus spp., Pangasius spp. and murrels are among the favourite cage-reared fishes. Tilapia aurea and Tilapia nilotica have also proved to be well adapted to cage culture. Polyculture is only rarely practised.Floating cages seem to be the best suited for fish culture in most cases. Net cages are the simplest in design and the cheapest to build. The escape of fish, however, always remains an eventuality which might lead to choosing more expensive designs. Maintenance of cages must be regular. For flexibility of exploitation, cages should not be too large. Their location within the water body can play an important role in keeping production high.At high stocking rates, artificial feeding of a well-adapted diet is a necessity. Optimum conversion rates require a well-adjusted daily ration, possibly fractioned. Mechanized feed distribution is to be encouraged as a labour-saving practice. Less intensive systems rely more on locally available wastes, such as trash fish, but the feeding rate then depends mostly on feed prices.Best growth is generally achieved by C. carpio and I. punctatus. Attainable maximum production is as high as 35 kg/m³/month under intensive management. However, generally, production averages from 5 to 30 kg/m³/month for these two species. S. gairdneri is less productive. T. aurea and T. nilotica have both a very good potential. Production in less intensive systems varies greatly according to management practices.     

Cypermethrin and λ-cyhalothrin induced alterations in nucleic acids and protein contents in a freshwater fish, <Emphasis Type="Italic"> Channa punctatus</Emphasis>

In this study, a freshwater fish Channa punctatus was exposed to subacute concentrations of synthetic pyrethroid insecticides (cypermethrin and λ-cyhalothrin) for 96 h to evaluate their impact on the levels of nucleic acids and protein in its different organs. Significant enhancement in the level of DNA was recorded in all tissues of the fish at high concentration of cypermethrin, whereas RNA and protein contents increased in tissues at all concentrations of cypermethrin tested. In contrast, λ-cyhalothrin treatment caused an increase in the level of DNA only in liver and brain, whereas increase of RNA and protein varied to different levels in different tissues. Cypermethrin treatment induced RNA/DNA ratio in all fish organs tested, whereas λ-cyhalothrin caused a sharp decrease in the ratio. Protein/DNA ratios were found to be tissue specific in treatments with both of the insecticides. The results clearly indicated that both of these pyrethroids exerted their effects in a similar manner in fish liver but differed in other tissues. These insecticides acted as potential biomodulators in C. punctatus, though following different routes. The results may be an indicator of aquatic pollution affecting freshwater fauna and flora and thus signaling the need for strict regulation on the indiscriminate input of pyrethroids from agricultural sites.     

Sea basses, Dicentrarchus labrax (Linne) and D. punctatus (Bloch) (Pisces,Serranidae), a control fish in fresh water

Dicentrarchus labrax and Dicentrarchus punctatus is able to thrive and grow in fresh water ponds. In all experiments there was successful controlling of young Tilapia aurea in the experimental ponds in comparison to the control ones. The number of young T. aurea per female and per pond was significantly lower in the experimental ponds than in control ponds. In 1973 the average number of young T. aurea per female was 80 in the experimental ponds and 287 young in the control ponds. In 1974 the average number of T. aurea young per female was 48 in the experimental ponds and 586 young in the control ponds.The growth rate of D. punctatus and D. labrax was higher in fresh water ($\text{S = 0.5‰}$) than in nature (salt water).     

Potential impacts of non‐native channel catfish on commercially important species in a Japanese lake,as inferred from long‐term monitoring data

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Culture environment and the odorous volatile compounds present in pond-raised channel catfish (<Emphasis Type="Italic">Ictalurus punctatus</Emphasis>)

A headspace solid-phase micro-extraction (SPME) coupled with GS-MS method was used to measure volatile compounds in fillets from musty off-flavor, muddy off-flavor, and on-flavor channel catfish (Ictalurus punctatus), along with water and soil samples from the farm ponds in which the fish had been raised. Two ponds of each type of flavor were selected, and five fish, water, and soil samples were collected from each pond. Linear and multiple linear regression analyses were carried out between/among off-flavor strength and volatile compound contents to investigate their possible correlations. The combination of two strong off-flavor compounds, 2-methylisoborneol (MIB) and geosmin (GSM), was probably mainly responsible for the musty off-flavor in the catfish fillets, and an odorous alcohol, 1-hexanol, was correlated with muddy off-flavor (p =?0.015). There was a strong correlation between beta-cyclocitral and MIB in a pond that gave musty off-flavor catfish contents (p =?0.006), suggesting that these compounds might be generated by similar cyanobacteria. The contents of GSM, MIB, and beta-cyclocitral were high in the water of ponds that yielded off-flavor fish, indicating that catfish might acquire these compounds from pond water.     

黄河入海泥沙年际通量对莱州湾渔业资源结构的影响

根据2009年8月-2013年8月莱州湾海域渔业拖网调查数据,分析该海域渔业种群组成、优势种、空间分布和资源量的年际变动,以了解黄河入海泥沙通量对莱州湾渔业结构的影响.结果表明:(1)莱州湾渔业资源量整体呈下降趋势,主要以鱼类为主,甲壳类次之,头足类最少;鱼类中以中上层鱼类为主,网获量明显多于底层鱼类,但2011年8月底层鱼类稍多于中上层鱼类;(2)莱州湾渔业资源各年份的优势种有所变动,中上层鱼类和底层鱼类的优势种变动较大,甲壳类优势种变动较少,鱼类优势种组成存在年际更替现象,主要由经济价值高、个体大的种类向经济价值低、个体小的种类演替.主要优势种集中于斑鲦、矛尾鰕虎鱼、口虾蛄、小带鱼和日本枪乌贼等种类.2010年斑鲦大量暴发,为唯一优势种;(3)2009-2013年黄河入海泥沙年际通量与鱼类种数、底层鱼类数和渔业资源数存在显著相关性,与底层鱼类生物量存在极显著相关性.综上所述,黄河入海泥沙年际通量的变动对莱州湾渔业资源量和资源结构都产生了一定影响.     

Removal and utilization of nutrients by Chinese waterchestnut in catfish ponds

The effect of Chinese waterchestnut (Eleocharis dulcis) on production of channel catfish (Ictalurus punctatus) and resulting water quality were studied in 1975 in 0.04-ha earthen ponds at Clemson University. Ponds containing waterchestnut had significantly lower levels of nitrate nitrogen and ammonia nitrogen. These lower nitrient levels resulted in lower phytoplankton levels in ponds with waterchestnut.Data indicated that Chinese waterchestnut had no effect on channel catfish production. Poor fish survival may have prevented detection of any effect of waterchestnut on catfish production.Chinese waterchestnut removed nutrients from the pond water. Mean nutrient removal per ha for the 201-day growing season was 108.06 kg of nitrogen, 6.90 kg of calcium, and 37.46 kg of magnesium. Mean corm production was 4664 kg per ha. These data indicate that Chinese waterchestnut should remove nutrients in sufficient quantities to improve water quality and allow increases in feeding rates and greater catfish production.     

Assessment of spermatogenesis and plasma sex steroids in a seasonal breeding teleost: a comparative study in an area of influence of a tributary, downstream from a hydroelectric power dam, Brazil

River damming and building of hydroelectric power plants interrupt the reproductive migration routes and change the major physicochemical parameters of water quality, with drastic consequences for populations of migratory fishes. The goal of this study was to evaluate proliferation and cell death during spermatogenesis and serum profiles of sex steroids in Prochilodus argenteus, from the S?o Francisco River, downstream from the Três Marias Dam. A total of 257 adult males were caught quarterly during a reproductive cycle in two sites: the first 34?km of the river after the dam (site 1) and the second 34–54?km after the dam (site 2), after the confluence with a tributary, the Abaeté River. Seasonal changes in the testicular activity associated with morphometric analyses of germ cells as well as proliferation and testicular apoptosis support a more active spermatogenesis in fish from site 2, where higher levels of sex steroids and gonadosomatic index (GSI) were also found. In site 1, fish presented low serum levels of testosterone, 17β-estradiol and 17α-hydroxyprogesterone and a low GSI during gonadal maturation. Spermatogonial proliferation (PCNA) and apoptosis (TUNEL) were more elevated in fish from site 1, but spermatocytes were mainly labelled in fish from site 2. Overall, these data demonstrate changes in testicular activity and plasma sex steroids in a neotropical teleost fish living downstream from a hydroelectric dam, supplying new data on fish reproduction in regulated rivers. Moreover, morphometric analyses associated with sex steroids profiles provide reliable tools to assess fish spermatogenesis under environmental stress conditions.     

Evaluation of potassium ricinoleate as a selective blue-green algicide in channel catfish ponds

The effectiveness of potassium ricinoleate as a selective blue-green algicide was investigated in experimental channel catfish, Ictalurus punctatus, culture ponds. Five ponds were treated with 0.8 mg/l potassium ricinoleate three times per week from May through October and five ponds served as controls. Treatment did not affect concentrations of chlorophyll-a, total and un-ionized ammonia-nitrogen, or dissolved oxygen; nor did it affect average hours of supplemental aeration or net fish production. The mean nitrite-nitrogen concentration in control ponds was higher (P<0.05) than that in treated ponds, but concentrations of nitrite never reached levels considered detrimental to the health of the fish. Treatment did not reduce the incidence or percentage of blue-green algae in phytoplankton communities and did not prevent severe episodes of off-flavor in fish from treated ponds.     

Hydrogen peroxide,iodine solution and methylene solution highly enhance the hatching rate of freshwater ornamental fish species

The aim of this study was to evaluate the effect of hydrogen peroxide, iodine solution (PVP) and methylene blue on eggs disinfection of three ornamental fish species, Danio rerio, Pterophyllum scalare and Gymnocorymbus ternetzi. The main idea was to create conditions to enhance the hatching rates. Eggs of each species were exposed to different concentrations of hydrogen peroxide (5, 10, 15 and 25 mg/L), PVP (0.25, 0.5, 0.75 and 1 mg/L) and methylene blue (0.5 1, 2 and 3 mg/L). The optimal doses ranged between species and chemicals: for G. ternetzi, the concentrations that high enhanced the hatching rate were 1 mg/L for the PVP treatment, 25 mg/L for the hydrogen peroxide treatment and 3 mg/L for methylene blue treatment; for P. scalare, the best results were achieved with 25 mg/L for hydrogen peroxide treatment and 3 mg/L for methylene blue treatment. By contrast, for all the different chemical did not increased the D. rerio hatching rate. Results showed that hydrogen peroxide and methylene blue are the most versatile, effective and safe to use in these species. On the other hand, PVP can be used but with many precautions due to very low safety margin. Results clearly show that the optimal concentration of chemicals for eggs disinfection is fish species dependent and it is completely wrong to extrapolate concentrations between different chemicals and fish species. Our study suggests that P. scalare can be used as a model in study of effectiveness of new chemicals with potential to disinfect water and increase hatching rates.     

Histopathological alterations in Senegal sole, Solea Senegalensis, from a polluted Huelva estuary (SW, Spain)

As a component of a large research project to evaluate the effects of contaminants on fish health in the field, histopathological studies have been conducted to help establish causal relationship between pollutants (heavy metals and aromatic polycyclic hydrocarbons—PAHs) and histopathological responses in Senegal sole, Solea senegalensis, from an estuary of SW Spain. Heavy metals (As, Zn, Cd, Pb, Cu and Fe) and 16 PAHs (proprietary USEPA) concentrations in water, sediment and tissues (liver and gills) and histopathological alterations in S. senegalensis from three sampling sites of Ria de Huelva estuary during 2004–2006 years have been analysed. The histopathological studies revealed seasonal and spatial differences in the lesion grade of alterations observing the highest lesion grades in fish from Odiel River and autumn season. No significant differences were observed in the alterations prevalence between sampling sites, but significant differences were observed between seasons observing the highest prevalence in autumn season. However, calculated IPAT demonstrated a low–moderate impact of pollutants on health fish. Correlations between histopathological alterations and pollutants analysed were observed being heavy metals the group that presented a major number of correlations with alterations in several organs of S. senegalensis. In evaluating the general health of fish, the use of histopathological studies in recommended for making more reliable assessment of biochemical responses in fish exposed to a variety of environmental stressors. Statistical analysis using semiquantitative data on pathological lesions can help to establish correlation between cause (stressor) and effect (biomarker).     

Approximate Water and Chemical Budgets for an Experimental,In‐pond Raceway System

There is increasing interest in intensive production of Ictalurid catfish in the United States and a better understanding of water quality dynamics in intensive culture is needed. Budgets for water, nitrogen, and phosphorus were estimated over a production season (March–November) for an In‐pond Raceway System for channel catfish, Ictalurus punctatus, and hybrid catfish, I. punctatus×I. furcatus, with co‐culture of paddlefish, Polyodon spathula, and Nile tilapia, Oreochromis niloticus. In addition to the rainfall and runoff, 70 cm of water was applied from a well to offset evaporation and seepage. Production of each kilogram of live catfish required 1.50 kg of feed and released 51.7 g nitrogen and 9.7 g phosphorus. Harvest of catfish accounted for 34.0% of nitrogen and 37.1% of phosphorus applied in feed. Seepage and overflow removed only small portions of nitrogen and phosphorus, while denitrification and ammonia volatilization removed large amounts of nitrogen. Some nitrogen accumulated in sediment. Phosphorus was harvested in fish and absorbed by pond sediment. Mechanical aeration aided in maintaining appropriate dissolved oxygen levels for fish production.