The Effect of N Deposition on Nitrous Oxide and Nitric Oxide Emissions from Temperate Forest Soils

Long-term and short-term N deposition effects on N₂O and NO emissions from forest soils were compared. Long-term NH₃ deposition (> 20 years) from a poultry farm to a downwind woodland (decreasing from 73 to 18 kg N ha^-1 y^-1, 30 to 110 m downwind of the farm) resulted in the re-emission of 6% and 14% of NH₃-N deposited as N₂O-N and NO-N, respectively. However, when in short-term (2-3 years) field experiments the atmospheric N deposition to mature conifer plantations was raised by fumigation with NH₃ to 15 kg N ha^-1 y^-1 or by acid mist to 48 and 96 kg N ha^-1 y^-1 the N deposited was immobilised. In the acid mist experiment more than 2 years of acid mist (48 and 96 kg N ha-1 y^-1) were required to significantly increase N₂O emissions from -0.3 μg N₂O-N m^-2 h^-1 (control) to 0.5 and 5.7 μg N₂O-N m^-2 h^-1, respectively. This suggests, that N deposition simulation studies in soil ecosystems, which have previously not been exposed to high rates of N (by deposition or fertilisation), need to be long-term. Also, measurements of N₂O and/or NO may be a non-destructive, quick indicator of the N status of the soil.     

有机无机肥料配施对春玉米农田N2O排放及净温室效应的影响

为了明确有机无机肥料配施条件下华北旱地春玉米农田N2O周年排放规律、影响因素及其净温室效应,采用静态箱-气相色谱法和生物地球化学模型（DNDC）相结合的方法,对单施化肥（NPK）、有机无机肥料配施（50％M＋50％U）、单施有机肥（M）、对照（CK）等处理的春玉米农田N2O排放情况进行了周年监测,并对DNDC模型进行验证,利用验证后的模型定量评价了不同施肥处理的净温室效应。结果表明：不同有机无机肥料配施处理N2O放通量具有明显的季节变化规律,通量变化范围是-17．56—157．25μg·m2·h-1,在非生长季观测到明显的N2O排放峰,最大排放通量为83．85μg·m2·h-1。NPK、50％M＋50％U、M、CK处理周年累计排放量分别为1．49、1．20、0．82、0．61kgN·hm-2·a-1,非生长季排放总量分别占全年总排放量的40．6％、59．2％、61．7％和60．7％,非生长季N2O排放不容忽视;在整个周年观测期内,当土壤水分含量介于19％-37％之间时,各处理下的N2O通量同土壤含水量呈极显著正相关关系。综合考虑整个农田生态系统碳收支平衡和温室气体排放,经过DNDC模型模拟表明有机无机肥料配施同单施化肥处理相比净温室效应减少33．5％,可以达到在保持产量的基础上“减排”和“固碳”的协同效果。上述研究结果为有机无机肥料合理使用以及旱地农田“稳产、减排、固碳”相协调施肥技术的筛选提供了科学依据。     

冬小麦/大葱轮作体系N_2O排放特征及影响因素研究

采用静态暗箱-气相色谱法研究了冬小麦/大葱轮作体系不同施肥处理下农田N2O排放特征及排放系数,分析了土壤湿度和土壤温度等环境因子对N2O排放的影响。结果表明,农田N2O排放高峰值主要出现在每次施肥＋灌溉或强降雨之后的一段时间,大葱生长季排放峰值高且出现的频率比小麦生长季密集;N2O排放通量变化范围为-3.85～507.11μg N·m-2·h-1,平均值为251.63μgN·m-2·h-1,对于不同施肥处理,其年度N2O排放总量介于1.71 kg N·hm-2到4.60 kg N·hm-2之间。整个轮作体系不同处理N2O排放系数介于0.31%到0.48%之间,均值为0.43%;相对比农民习惯（FP）处理,优化施肥（OPT）、优化减氮（OPT-N）以及秸秆还田（C/N）处理均能显著减少N2O的排放,秸秆还田处理和优化减氮处理N2O排放总量比优化处理分别减少了17%和10%。在10℃〈土壤温度（T）s〈20℃时,N2O排放随温度的升高而增加;整个小麦生长季N2O排放随土壤湿度的增加而增加,且达到0.05的显著水平;大葱生长季在20℃〈Ts〈30℃时,土壤水分含量成为主要限制因素,N2O排放与土壤孔隙含水量（WFPS）呈显著指数正相关关系。秸秆还田处理作物产量高于其他处理,是具有减排增产＂双赢＂效果的农田管理措施。     

不同氮磷肥施用对春玉米农田N_2O排放的影响

农田过量施肥会增加N2O排放,使农田土壤成为重要的温室气体排放源。为减少农田N2O排放,利用自动观测系统研究了春玉米农田中不同肥料对N2O排放的影响,并结合作物产量及N2O的排放量探索减少温室气体排放的施肥措施。采用田间试验方法设定了不施肥（CK）、尿素（U）、尿素加磷肥（NP）和硝酸磷肥（NOP）4个处理进行研究。结果表明,各处理下N2O排放总量分别为：CK0.21kgN·hm-2、U1.19kgN·hm-2、NP0.93kgN·hm-2、NOP0.69kgN·hm-2;N2O排放主要受施肥、灌溉,降雨和土壤温度的影响;在作物生长后期土壤含氮量小于7mgN·kg-1的情况下,观测到土壤吸收N2O的情况;各处理下排放因子均小于政府间气候变化委员会（IPCC）的缺省值1%,表明IPCC推荐的排放因子不适用于估算中国北方的春玉米农田N2O排放。施加磷肥有助于减少农田N2O排放并提高产量,硝态磷肥较尿素可以显著减少N2O排放。综合考虑产量和N2O排放,相对于施用尿素和尿素加磷肥处理,硝酸磷肥处理不仅可节约15%和30%的肥料投入,而且分别减少42%和26%的N2O排放,具有减排不减产的良好效果。     

Influence of Watering Methods on the Short-Term Pulse Emissions of Nitrous Oxide from Disturbed Horticultural Soil

To reveal the impact of soil disturbance and surface watering (SW) following soil disturbance on the pulse nitrous oxide (N₂O) emissions, incubated experiments were conducted on disturbed soil with two watering regimes [surface watering only (SWO) and subsurface watering followed by surface watering (SUW+SW)]. Intensive soil disturbance led to pulses N₂O emissions from SUW + SW soil (>8,693 μg N₂O m^?2 h^?1 with a peak of 30,938 μg N₂O m^?2 h^?1), although the water-filled pore space (WFPS) was substantially lower than the previously reported optimal soil moisture range (45–75% WFPS) for peak N₂O emissions. N₂O emissions from the disturbed soil after SW were much lower than those from SUW + SW soil, increased as the soil dried, and peaked when the WFPS fell within the optimal soil moisture range. These peaks were considerably less than those resulting from the intensive disturbance in SUW + SW soil. Thus, SW after intensive soil disturbance may be effective for mitigating of pulse N₂O emissions caused by soil disturbance.     

Emissions of trace gases (CO2, CO,CH4, and N2O) resulting from rice straw burning

Emissions of trace gases (CO₂, CO, CH₄, N₂O) resulting from rice straw burning were measured by using the open chamber method. The carbon contained in rice straw was mainly released to the atmosphere as CO₂. The percentage of CO₂-C emitted in total C in rice straw was in the range of 57–81%, followed by CO-C (5–9%). The percentages of CH₄-C and N₂O-N in total C and N in rice straw were in the range of 0.43–0.90 and 1.16–1.50%, respectively. In the case of the rice straw which had been left in the field for a period of one month after harvest, emission of imperfect combustible gases such as CO and CH₄ during burning increased slightly, while that of perfect combustible gas, CO₂, was reduced. The amount of CH₄ emission from rice straw burning was comparable to that from paddy fields.     

长期秸秆还田对设施菜田土壤反硝化特征和N2O排放的影响

基于2004年2月-2010年9月温室菜田长期定位试验,通过室内培养和田间同步,利用静态箱法和硅胶管法分别检测土壤表层N2O通量和剖面N2O浓度的变化,以研究高碳氮比的小麦秸秆施用对设施菜田土壤反硝化过程及N2O排放的影响.结果表明,（1）与对照处理（CK）相比,添加秸秆处理（ST）显著提高0-20cm土层土壤反硝化量,促进N2O还原,增加N2产生量,显著降低追肥灌溉后表层土壤N2O的排放峰值和土壤底层50cm处N2O浓度峰值,但对20-80cm土层土壤的反硝化特征影响较少.（2）秸秆还田有利于降低设施菜田NO;淋洗风险,秸秆的深施是进一步降低菜田NO3-淋洗的有效途径,有利于土壤底层N2O的再次还原.因此,设施菜田中添加小麦秸秆并深施有利于降低N2O排放和减少NO3-的淋洗.     

Emissions of Greenhouse Gases CH4 and N2O from Low-gradient Streams in Agriculturally Developed Catchments

Lentic wetlands are usually regarded as the most important natural freshwater sources of methane (CH₄) and nitrous oxide (N₂O) to the atmosphere, and very few studies have quantified the importance of lowland streams in trace gas emissions. In this study, we estimated fluxes of CH₄ and N₂O in three macrophyte-rich, lowland agricultural streams in New Zealand, to place their trace gas emissions in context with other sources and investigate the value of minimising their emissions from agricultural land. All three streams were net sources of both gases, with emission of CH₄ ranging from <1 to 500 μmol m^?2 h^?1 and of N₂O ranging from <1 to 100 μmol m^?2 h^?1 during mid-summer. For CH₄, both turbulent diffusion across the surface and ebullition of sediment gas bubbles were important transport processes, with ebullition accounting for 20–60% of the emissions at different sites. The emissions were similar on a per area basis to other major global sources of CH₄ and N₂O. Although small on a catchment scale compared to emissions from intensively grazed pastures, they were significant relative to low-intensity pastures and other agricultural land uses. Because hydraulic variables (viz. depth, velocity and slope) strongly influence turbulent diffusion, complete denitrification can best proceed to N₂ as the dominant end-product (rather than N₂O) in riparian wetlands, rather than in open stream channels where N₂O fluxes are sometimes very large.     

外加可溶性碳源对华北典型农田土壤N2O、CO2排放的影响

以华北平原典型农田土壤为对象,运用静态培养系统研究方法,设置室内培养试验,研究添加不同浓度葡萄糖对土壤N2O、CO2排放的影响.结果表明:碳氮配施的外源添加方式明显促进N2O和CO2排放,其排放通量均高于对照组和只添加氮源的处理.在配施碳源葡萄糖浓度为0.5 g/kg时N2O排放通量最高(NH4+组2 500 μg/(kg·d),单位以N计,下同,NO3-组1 500 μg/(kg·d)),4.0 g/kg时N2O排放通量最低(NH4+组500 μg/(kg·d),NO3-组800 μg/(kg·d));葡萄糖浓度为2.0 g/kg时CO2排放通量最高(NH+组500mg/(kg· d)),0.5 g/kg时CO2排放通量最低(NH+组100 mg/(kg,d)).从培养开始到结束,只添加氮源的土壤NH+含量变化不明显,NO3-含量增至29.21 mg/kg(NH4+组)和62.25 mg/kg(NO3-组);而配施葡萄糖的土壤NH+含量降为不足1 mg/kg(NH4+组),NO3-含量明显减少.N2O累积排放通量与葡萄糖浓度呈负相关(NH4+组),CO2累积排放通量与葡萄糖浓度呈正相关.分析结果表明,外加可溶性碳源明显减少土壤中NH4+和NO3-含量,并且促进土壤N2O、CO2排放,其排放通量大小与C/N比有关.     

添加生物炭对华南早稻田CH4和N2O排放的影响

通过田间试验,采用静态箱-气象色谱法研究生物炭添加对华南早稻田甲烷(CH4)和氧化亚氮(N2O)排放的影响.试验设对照(CK),BC1、BC2、BC3(土壤中分别混入5、10、20t·hm-2生物炭)、RS(稻草直接还田)和RI(稻草加腐熟剂还田)6个处理.结果表明,与CK、RS和RI相比,生物炭处理能降低稻田CH4和N2O排放量及排放强度,综合排放强度最低的为BC3处理,值为0.98 kgCO2-eq· kg-1.在本实验的处理范围内,生物炭添加量越高,CH4平均排放通量及其季节排放总量越低,最低值分别为22.11mg·m-2·h-1和93.21kg·hm-2;N2O排放通量和季节排放总量则随生物炭添加量的增加呈上升趋势,最高值分别为285.65 μg·m-2·h-1和1.07kg·hm-2,但依然小于对照处理(368.13μg·m-2 ·h-1和1.13kg·hm-2).此外,试验发现高用量的生物炭(即BC3)处理水稻产量最高,值为7152.58kg·hm-2.综合分析各处理,BC3对减缓温室效应和稳定水稻生产贡献最大.     

N2O, NO, and NH3 Emissions from Soil after the Application of Organic Fertilizers, Urea and Water

Agricultural soil is a major source of nitrous oxide (N₂O), nitric oxide (NO) and ammonia (NH₃). Little information is available on emissions of these gases from soils amended with organic fertilizers at different soil water contents. N₂O, NO and NH₃ emissions were measured in large-scale incubations of a fresh sandy loam soil and amended with four organic fertilizers, [poultry litter (PL), composted plant residues (CP), sewage sludge pellets (SP) and cattle farm yard manure (CM)], urea fertilizer (UA) or a zero-N control (ZR) for 38 days. Fertilizers were added to soil at 40, 60 or 80% water-filled pore space (WFPS). The results showed that urea and organic fertilizer were important sources of N₂O and NO. Total N₂O and NO emissions from UA ranged from 0.04 to 0.62%, and 0.23 to 1.55% of applied N, respectively. Total N₂O and NO emissions from organic fertilizer treatments ranged from 0.01 to 1.65%, and <0.01 to=" 0.55%=" of=" applied=" n,=" respectively.=" the=" lower=">₂O and NO emissions from CP and CM suggested that applying N is these forms could be a useful mitigation option. Comparison of the NO-N/N₂O-N ratio suggested that nitrification was more dominant in UA whereas denitrification was more dominant in the organic fertilizer treatments. Most N was lost from PL and UA as NH₃, and this was not influenced significantly by WFPS. Emissions of NH₃ from UA and PL ranged from 62.4 to 69.6%, and 3.17 to 6.11% of applied N, respectively.     

N2O,NO, and NH3 Emissions from Soil after the Application of Organic Fertilizers,Urea and Water

Water, Air, & Soil Pollution - Agricultural soil is a major source of nitrous oxide (N2O), nitric oxide (NO) and ammonia (NH3). Little information is available on emissions of these gases from...     

Characteristics of Raindrop Acidification by Co-Washout of SO2(G)-H2O2(G)-HNO3(G)

In order to investigate the acid rain formation under the coexistence of SO₂(g), H₂O₂(g), and HNO₃(g) in the air, a mathematical model has been built and some numerical simulations have been carried out with use of the model. The simulation reveals that SO₂(g) absorbed into a raindrop is released and then re-absorbed as the fall distance increases. The desorption and re-absorption processes of SO₂(g) are caused by: (1) the fact that the equilibrium concentration of H₂O₂(aq) and HNO₃(aq) in raindrops are much higher than that of SO₂(aq), and (2) the fact that the oxidation reaction rate of HSO₃ ^? with H₂O₂(aq) increases with H⁺ concentration in raindrops. The degree of acidification of the rainwater has been estimated by introducing a raindrop size distribution. The acidification is mainly caused by the adsorption of SO₂(g) in the usual case where the atmospheric concentration of SO₂(g) is much higher than that of HNO₃(g). With the increase in the atmospheric concentration of HNO₃(g), the concentration of H⁺ generated from SO₂(g) decreases and the contribution of HNO₃(g) to the generation of H⁺ becomes dominant.

     

Nitrous oxide (N<Subscript>2</Subscript>O)-reducing denitrifier-inoculated organic fertilizer mitigates N<Subscript>2</Subscript>O emissions from agricultural soils

The only known sink for nitrous oxide (N₂O) is biochemical reduction to dinitrogen (N₂) by N₂O reductase (N₂OR). We hypothesized that the application of N₂O-reducing denitrifier-inoculated organic fertilizer could enhance soil N₂O consumption while the disruption of nosZ genes could result in inactivation of N₂O consumption. To test such hypotheses, a denitrifier-inoculated granular organic fertilizer was applied to both soil microcosms and fields. Of 41 denitrifier strains, 38 generated ³⁰N₂ in the end products of denitrification (³⁰N₂ and ⁴⁶N₂O) after the addition of Na¹⁵NO₃ in culture condition, indicating their high N₂O reductase activities. Of these 41 strains, 18 were screened in soil microcosms after their inoculation into the organic fertilizer, most of which were affiliated with Azospirillum and Herbaspirillum. These 18 strains were nutritionally starved to improve their survival in soil, and 14 starved and/or non-starved strains significantly decreased N₂O emissions in soil microcosms. However, the N₂O emission had not been decreased in soil microcosms after inoculating with a nosZ gene-disruptive strain, suggesting that N₂O reductase activity might be essential for N₂O consumption. Although the decrease of N₂O was not significant at field scales, the application of organic fertilizer inoculated with Azospirillum sp. TSH100 and Herbaspirillum sp. UKPF54 had decreased the N₂O emissions by 36.7% in Fluvisol and 23.4% in Andosol in 2014, but by 21.6% in Andosol in 2015 (H. sp. UKPF54 only). These results suggest that the application of N₂O-reducing denitrifier-inoculated organic fertilizer may enhance N₂O consumption or decrease N₂O emissions in agricultural soils.     

Effects of biochar and 3,4-dimethylpyrazole phosphate (DMPP) on soil ammonia-oxidizing bacteria and nosZ-N2O reducers in the mitigation of N2O emissions from paddy soils

Purpose

Paddy fields are an important source of nitrous oxide (N₂O) emission. The application of biochar or the nitrification inhibitor 3,4-dimethylpyrazole phosphate (DMPP) to paddy soils have been proposed as technologies to mitigate N₂O emissions, but their mechanisms remain poorly understood.

Methods

An experiment was undertaken to study the combined and individual effects of biochar and DMPP on N₂O emission from a paddy field. Changes in soil microbial community composition were investigated. Four fertilized treatments were established as follows: fertilizer only, biochar, DMPP, and biochar combined with DMPP; along with an unfertilized control.

Results

The application of biochar and/or DMPP decreased N₂O emission by 18.9–39.6% compared with fertilizer only. The combination of biochar and DMPP exhibited higher efficiency at suppressing N₂O emission than biochar alone but not as effective as DMPP alone. Biochar promoted the growth of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB), while DMPP suppressed AOB and increased AOA. Applying biochar with DMPP reduced the impact of DMPP on AOB. The nirS-/nirK- denitrifiers were decreased and nosZ-N₂O reducers were increased by DMPP and the combination of DMPP and biochar. The abundance of the nirK gene was increased by biochar at the elongation and heading stages of rice development. Compared with fertilizer only, the application of biochar and/or DMPP promoted the abundance of nosZ genes.

Conclusion

These results suggest that applying biochar and/or DMPP to rice paddy fields is a promising strategy to reduce N₂O emissions by regulating the dynamics of ammonia oxidizers and N₂O reducers.

     

Evaluation of nitrate and ammonium as sources of NO and N2O emissions from black earth soils (Haplic Chernozem) based on 15N field experiments

Nitrous oxide (N₂O) and nitric oxide (NO) released from soil is a concern since it can act as a potential atmospheric pollutant and it represents a loss of N from the soil. These gases are present in the atmosphere in trace amounts and are important to atmospheric chemistry and earth's radiative balance. Nitric oxide (NO) does not directly contribute to the greenhouse effect, but it contributes to climate forcing through its role in photochemistry of hydroxyl radicals and ozone and plays a key role in air quality issues. Nitrification and denitrification have been identified as major controlling microbial processes in soils responsible for the formation of NO and N₂O. To elucidate the contribution of both processes to the release of NO and N₂O from loess-black earth soils under field conditions—i.e. to evaluate nitrate and ammonium as sources of NO and N₂O emission—two field experiments with either [¹⁵N] nitrate (NO₃^?) or [¹⁵N] ammonium (NH₄⁺) labelling have been conducted at two sites differing in soil organic matter content (high and normal SOM). [¹⁵N] nitrate treatments revealed that denitrification of NO₃^? represents the main pathway of soil N₂O release. On average 76% and 54% of N₂O was emitted during denitrification from soils with high and normal SOM content, respectively. Contrarily, denitrification contributed on average only 17% and 12% of released NO from soil with high and normal SOM content, respectively. The [¹⁵N]ammonium treatments revealed that nitrification of NH₄⁺ is the major process responsible for soil NO emission. SOM content of the loess-black earth soil significantly influenced NO and N₂O emission. The soil with the higher SOM content showed lower NO emission but drastically increased N₂O emission after nitrate fertilisation. In particular the soil with high SOM content exhibited a high sorption capacity for ammonium ions which led to unexpected results after fertilisation with [¹⁵N]ammonium. To explain this results a revised concept containing three different interacting soil ammonium pools have been hypothesised.     

Oxygen and substrate availability interactively control the temperature sensitivity of CO2 and N2O emission from soil

Biology and Fertility of Soils - We investigated how oxygen availability, substrate amount, and quality affect the temperature dependency of enzymatic processes involved in the production of carbon...     

Influence of pH on the release of NO and N2O from fertilized and unfertilized soil

Summary NO and N₂O release rates were measured in an acidic forest soil (pH 4.0) and a slightly alkaline agricultural soil (pH 7.8) after the pH was adjusted to values ranging from pH 4.0 to 7.8. The total release of NO and N₂O during 20 h of incubation was determined together with the net changes in the concentrations of NH ₄ ⁺ , NO ₂ ^– and NO ₃ ^– in the soil. The release of NO and N₂O increased after fertilization with NH ₄ ⁺ and/or NO ₃ ^– ; it strongly decreased with increasing pH in the acidic forest soil; and it increased when the pH of the alkaline agricultural soil was decreased to pH 6.5. However, there was no simple correlation between NO and N₂O release or between these compounds and activities such as the NO ₂ ^– accumulation, NO ₃ ^– reduction, or NH ₄ ⁺ oxidation. We suggest that soil pH exerts complex controls, e.g., on microbial populations or enzyme activities involved in nitrification and denitrification.     

硝态氮源及碳源有效性对土壤N_2O和CO_2排放的影响

以华北平原农田土壤为对象,通过室内静态培养系统研究NO_3~--N与不同碳源组合对土壤N_2O和CO_2排放的影响。结果表明,NO_3~--N作为氮源和不同碳源施入土壤,除NO_3~-+纤维素,其余土壤N_2O排放通量均高于对照组和只添加氮源土壤;NO_3~--N和不同碳源组合的CO_2累积排放量均高于对照和只添加氮源土壤。NO_3~-+果胶的N_2O排放量在第1 d达到最大值1 383.42μg N·kg~(-1)·d~(-1);NO_3~-+葡萄糖的CO_2排放量在第1 d达到最大值370.13 mg C·kg~(-1)·d~(-1),CO_2累积排放量顺序为:葡萄糖果胶秸秆纤维素淀粉木质素。土壤NO_3~--N含量与N_2O排放呈极显著正相关。总之,添加纤维素可以抑制N_2O的排放,促进CO_2排放,并增加土壤中NO_3~--N含量,添加其余碳源均会促进土壤N_2O和CO_2排放。