Influence of Integrated Supply of Vermicompost and Zinc‐Eniched Compost with Two Graded Levels of Iron and Zinc on the Productivity of Geranium

Abstract

Two pot experiments under greenhouse condition were carried out to study the influence of vermicompost and zinc‐enriched compost with two levels of iron and zinc on the productivity of geranium (Pelargonium graveolens). Joint application of vermicompost and zinc‐enriched compost was effective in increasing the herb and oil yield over sole application of iron and zinc. Combined application of vermicompost and zinc‐enriched compost gave better herb and oil yield in both the experiments. With application of vermicompost and zinc‐enriched compost with two graded levels of iron, higher N, P, and K concentrations were observed with application of vermicompost (5 g kg^?1), vermicompost (5 g kg^?1), and Fe 12.5 ppm+Zn‐enriched compost 2.5 g kg^?1 soil, respectively, over control. Highest reduction in soil pH was observed with an application of vermicompost at 5 g kg^?1 soil; maximum soil organic carbon content was also recorded in the same treatment. In experiment II, joint application of vermicompost, zinc‐enriched compost, and graded levels of zinc recorded highest N, P, and K concentration with treatments of Zn (15 ppm)+vermicompost (2.5 g kg^?1), vermicompost (5 g kg^?1), and Zn (15 ppm)+vermicompost (2.5 g kg^?1 soil), respectively. Nitrogen, P, and K content increased by 36, 125, and 305%, respectively, with these treatments over the control.

Chemical constituents of geranium oil such as cis‐rose oxide, isomenthone, linalool, citronellyl, geranylformate, geranyl, and epi‐γ‐eudesmol were significantly improved by combined application of Zn with vermicompost and Zn‐enriched compost as compared to sole application of Zn. Similar effects were observed with Fe in combination with vermicompost and Zn‐enriched compost on most of the chemical constituents of geranium oil. Physicochemical properties of the soil were also improved as macro‐ and micronutrient availability markedly increased in both the experiments because of combined application of vermicompost and Zn‐enriched compost with two levels of Zn and Fe.     

Influence of Chromium with Vermicompost on Growth and Accumulation by Brahmi

Abstract

A pot experiment was conducted under glasshouse conditions during 2004 on brahmi (Bacopa monnieri) at the Central Institute of Medicinal and Aromatic Plants (CIMAP) in Lucknow. The study was conducted to evaluate the influence of different levels of chromium (Cr), with and without vermicompost, on growth and yield, as well as on accumulation of Cr by Bacopa. Rooted cuttings of Bacopa were grown under three levels of Cr (10, 20, and 40 ppm), two levels of vermicompost (2.5 and 5 g kg^?1 soil), and a combination of both. The results indicated that herb yield increased with the joint application of vermicompost and chromium, as compared to compost and Cr alone. Application of Cr only decreased nitrogen (N) and increased phosphorus (P) concentration in plants with increase in its supply, whereas application with vermicompost raised N concentration. Chromium concentration in plant tissue was found to be highest at supply of Cr at 40 ppm+vermicompost 5 g kg^?1 soil. Increase in the supply of Cr only (10, 20, and 40 ppm) decreased iron (Fe), copper (Cu), and zinc (Zn) concentration in plants. The experiment suggests that brahmi, because of its high accumulation ability, could be used as a scavenger to clean Cr‐contaminated soil.     

Effect of Vermicompost and Chemical Fertilizer on Growth,Herb, Oil Yield,Nutrient Uptake,Soil Fertility,and Oil Quality of Rosemary

Essential oil of rosemary (Rosmarinus officinalis L.) possesses good olfactory properties and is suitable for use in perfumes, soaps, and fragrances. Field experiments were conducted for 2 years (2003?2005) in an area experiencing a semi-arid tropical climate to study the influence of vermicompost and chemical fertilizer on growth, herb, oil yield, nutrient uptake, soil fertility, and oil quality of rosemary. Results from the experiment revealed that among the seven treatments, the application of vermicompost (8 t ha^?1) + fertilizer nitrogen (N)?phosphorus (P)??potassium (K) (150:25:25 kg ha^?1) produced optimum herbage and oil yield of rosemary compared with control (no fertilizer) and was found to be on par with application of fertilizer NPK 300:50:50 kg ha^?1. Content and quality of oil were not influenced by vermicompost and chemical fertilizers. Furthermore, it was noticed that available N and P were greater in postharvest soils that received vermicompost alone or in combination with inorganic fertilizers than control (no fertilizer) and inorganic fertilizer?treated soil. This study indicates that combined application of vermicompost and chemical fertilizer helps to increase crop productivity and sustain the soil fertility.     

Inoculation of arbuscular mycorrhizal fungi can substantially reduce phosphate fertilizer application to Allium fistulosum L. and achieve marketable yield under field condition

The effects of inoculating arbuscular mycorrhizal (AM) fungi on the growth, phosphorus (P) uptake, and yield of Welsh onion (Allium fistulosum L.) were examined under the non-sterile field condition. Welsh onion was inoculated with the AM fungus, Glomus R-10, and grown in a glasshouse for 58?days. Non-inoculated plants were grown as control. Inoculated and non-inoculated seedlings were transplanted to a field with four available soil P levels (300, 600, 1,000, and 1,500?mg P₂O₅?kg^?1 soil) and grown for 109?days. AM fungus colonization, shoot P concentration, shoot dry weight, shoot length, and leaf sheath diameter were measured. Percentage AM fungus colonization of inoculated plants was 94% at transplant and ranged from 60% to 77% at harvest. Meanwhile, non-inoculated plants were colonized by indigenous AM fungi. Shoot length and leaf sheath diameter of inoculated plants were larger than those of non-inoculated plants grown in soil containing 300 and 600?mg P₂O₅?kg^?1 soil. Shoot P content of inoculated plants was higher than that of non-inoculated plants grown in soil containing 300 and 600?mg P₂O₅?kg^?1 soil. Yield (shoot dry weight) was higher for non-inoculated plants grown in soil containing 1,000 and 1,500?mg P₂O₅?kg^?1 soil than for those grown in soil containing 300 and 600?mg?P₂O₅ kg^?1 soil. Meanwhile, the yields of inoculated plants (200?g plant^?1) grown in soils containing the four P levels were not significantly different. Yield of inoculated plants grown in soil containing 300?mg P₂O₅ kg^?1 soil was similar to that of non-inoculated plants grown in soil containing 1,000?mg P₂O₅?kg^?1 soil. The cost of AM fungal inoculum for inoculated plants was US$ 2,285?ha^?1 and lower than the cost of superphosphate (US$ 5,659?ha^?1) added to soil containing 1,000?mg P₂O₅ kg^?1 soil for non-inoculated plants. These results indicate that the inoculation of AM fungi can achieve marketable yield of A. fistulosum under the field condition with reduced application of P fertilizer.     

Organic amendments and urea nitrogen effects the growth and nutrient content of fenugreek (Trigonella foenumgraecum) and goat pea (Securigera securidaca)

Abstract

In Iran, the recent spike in herbal medicines has led to a corresponding price increase. Therefore, these montane species are now widely field cultivated. Medicinal plant consumers strongly prefer organically grown plants, and farmers are responding to this market. However, the effects of organic amendments on medicinal plant nutrient content and yield have been poorly studied. Therefore, this study measured the effects of different organic treatments on the growth of fenugreek and goat pea. Plants were grown to compare the effects of composted municipal waste (CMW), vermicompost (VC), and urea nitrogen (N) to untreated field soil (C0). Germination percentage was significantly higher in urea N (76.5%), CMW (75.5%), and VC (51.6%) compared to C0 (36.3%). Fenugreek performed better in organic amendment treatments than goat pea. Urea N also increased fenugreek plant dry matter significantly compared to C0, but this increment was not as high as CMW or VC. Plants grown in VC had higher whole plant N content (3.2%) than those grown in CMW (2.8%) and plants grown in urea N (2.6%). Plants treated with CMW (8613?mg kg^?1) and VC (8503?mg kg^?1) had a significantly higher P content than those treated with N (7430?mg kg^?1) or C0 control grown (7236?mg kg^?1). Application of VC significantly increased plant K content (2483?mg kg^?1) compared to CMW (1850?mg kg^?1), N (1750?mg kg^?1), and C0 control (1716?mg kg^?1). Even without fertilization, both plant species contained a considerable amount of micronutrient elements.     

Self-generated microbial population or cultured microflora – an important criterion toward development of an effective and economically viable road map for organic soil management

Huge depletion of soil microflora under conventional farming practice has become the primary contributory factor toward the present depletion of soil and crop productivity. Reconstitution of soil microbial dynamics has been identified as the only way out, but there has been a debate regarding the most effective pathway for soil rejuvenation i.e. whether to create the environment for natural proliferation or opt for inoculation of laboratory generated microbes. In this respect, a study was undertaken at Maud T.E. (Assam) under FAO-CFC-TBI Project, where bio-fertilizer (microbial inoculant, MI), vermicompost (organic food source, OF), vermicompost + bio-fertilizer (OF+MI), and Novcom compost (representing self-generated native microflora in the order of 10¹⁶c.f.u. along with organic food source, SNM); were taken as treatments for a yield target of 1500 kg made tea/ha. The highest crop yield (1500 kg ha^?1) along with high and consistent soil quality development was noted under SNM treatment; while MI influenced lowest yield (1268 kg ha^?1) and minimal soil response. Addition of the organic food source with cultured microbes (MI+OF) was found to improve crop performance (1427 kg ha^?1), but with 7.60 times higher cost (Rs. 39.97 kg^?1 made tea). Economic viability study indicated that except SNM, all other treatments were vulnerable toward crop loss or market downfall.     

Heavy metals and arbuscular mycorrhizal (AM) fungi can alter the yield and chemical composition of volatile oil of sweet basil (<Emphasis Type="Italic">Ocimum basilicum</Emphasis> L.)

The effects of increasing levels of metals (10 and 20 mg of Cr kg^-1 and 25 and 50 mg of Cd, Pb, and Ni kg^-1 soil) and arbuscular mycorrhizal (AM) fungi Glomus intraradices on the yield, chemical composition of volatile oil, and metal accumulation in sweet basil (Ocimum basilicum L.) were investigated in a pot experiment. The shoot yield, content of essential oil, and root yield of sweet basil were increased by the application of low dose of Cd, Pb, and Ni as compared to control. The application of high level of metals had deleterious effect on the yield. In soil with low dose of metal applied, AM fungi inoculation significantly enhanced the metal concentration in shoots and had adverse effect on the yield, whereas in soil with high dose of metal applied, AM fungal inoculation reduced the metal concentration in shoot and had beneficial effect on the yield. The content of linalool in basil oil was decreased and that of methyl chavicol was increased by the application of Cr, Cd, and Pb in soil as compared to control. Similarly, the level of linalool and methyl chavicol was decreased and that of methyl eugenol was increased by the application of Ni as compared to control. However, AM fungal inoculation led to maintain the content of linalool, methyl chavicol, and methyl eugenol in volatile oil, which were either increased or decreased by the application of metals. We conclude that the AM–sweet basil symbiosis could be used as a novel approach to enhance the yield and maintain the quality of volatile oil of sweet basil under metal-contaminated soils.     

Arsenic accumulation and speciation in Japanese paddy rice cultivars

We examined arsenic (As) accumulation and speciation in the major cultivars currently grown in Japan, because differences in grain As levels among cultivars may influence dietary As exposure in Japanese people. Ten major cultivars (Oryza sativa L.) were grown under flooded conditions in a paddy field with a background level of As (low-As soil) or in pots filled with soil containing a high level of As (high-As soil). In the low-As soil, the total grain As ranged from 0.11 to 0.17?mg?kg^?1, with a mean concentration of 0.14?mg?kg^?1, and inorganic As was the major species in all cultivars. There were few genotypic differences in the levels of either total As or inorganic As in the grain. In the high-As soil, total grain As increased to a mean level of 2.4?mg?kg^?1 in the 10 cultivars, with markedly increased levels of dimethylarsinic acid. The genotypic variations among cultivars in the levels of both total As and dimethylarsinic acid were statistically significant. However, the genotypic variability of inorganic As levels was quite small, and these levels remained low (at about 0.2?mg?kg^?1) even when total As levels increased markedly. These results suggest that differences in grain As levels among Japanese cultivars may not influence dietary As exposure, because there is little genotypic difference in the accumulation of inorganic As, which is considered more toxic than organic As to humans. We discuss the possible mechanism of As accumulation in Japanese paddy rice, in the context of the accumulation of As species in the developing grain and in other plant tissues.     

Effects of Vermicompost Tea (Aqueous Extract) on Pak Choi Yield,Quality, and on Soil Biological Properties

This study investigated the effects of vermicompost tea (aqueous extract) on yield and chemical quality of pak choi (Brassica rapa cv Bonsai, Chinensis group) grown in three media (two soils and a peat-perlite medium) under two fertilizer regimes (compost and synthetic fertilizer). The impacts of tea application on the chemical and biological properties of the growth media were also investigated. Vermicompost teas were prepared using various extraction methods (non-aerated, aerated, aerated with additives) with 1:10 (v:v) chicken manure-based vermicompost to water dilution and applied weekly at the rate of 200 mL plant^?1 for 4 weeks. Application of vermicompost tea increased plant production, total carotenoids and total glucosinolates in plant tissue. This effect was most prominent under compost fertilization. Total phenolic was lower in vermicompost tea treated plants compared to those treated with only mineral nutrient solution and the water control. Vermicompost tea improved mineral nutrient status of plants and media, and enhanced the biological activity of the media. Variability in yield and chemical quality of plants across treatments was explained largely by variability in tissue N uptake and dry matter accumulation. Dehydrogenase activity and soil respiration of vermicompost tea-treated growth media were approximately 50% higher than untreated media. This study confirmed that vermicompost tea can positively influence plant yield and quality and increase soil biological activity in multiple soil types.     

Coffee‐husk biochar application increased AMF root colonization,P accumulation,N2 fixation,and yield of soybean grown in a tropical Nitisol,southwest Ethiopia

Low soil fertility and soil acidity are among the major bottlenecks that limit agricultural productivity in the humid tropics. Soil management systems that enhance soil fertility and biological cycling of nutrients are crucial to sustain soil productivity. This study was, therefore, conducted to determine the effects of coffee‐husk biochar (0, 2.7, 5.4, and 16.2 g biochar kg^?1 soil), rhizobium inoculation (with and without), and P fertilizer application (0 and 9 mg P kg^?1 soil) on arbuscular mycorrhyzal fungi (AMF) root colonization, yield, P accumulation, and N₂ fixation of soybean [Glycine max (L.) Merrill cv. Clark 63‐K] grown in a tropical Nitisol in Ethiopia. ANOVA showed that integrated application of biochar and P fertilizer significantly improved soil chemical properties, P accumulation, and seed yield. Compared to the seed yield of the control (without inoculation, P, and biochar), inoculation, together with 9 and 16.2 g biochar kg^?1 soil gave more than two‐fold increment of seed yield and the highest total P accumulation (4.5 g plant^?1). However, the highest AMF root colonization (80%) was obtained at 16.2 g biochar kg^?1 soil without P and declined with application of 9 mg P kg^?1 soil. The highest total N content (4.2 g plant^?1) and N₂ fixed (4.6 g plant^?1) were obtained with inoculation, 9 mg P kg^?1, and 16.2 g biochar kg^?1 soil. However, the highest %N derived from the atmosphere (%Ndfa) (> 98%) did not significantly change between 5.4 and 16.2 g kg^?1 soil biochar treatments at each level of inoculation and P addition. The improved soil chemical properties, seed yield, P accumulation and N₂ fixation through combined use of biochar and P fertilizer suggest the importance of integrated use of biochar with P fertilizer to ensure that soybean crops are adequately supplied with P for nodulation and N₂‐fixation in tropical acid soils for sustainable soybean production in the long term.     

Avian Urine: Its Potential as a Non-Invasive Biomonitor of Environmental Metal Exposure in Birds

Current non-invasive biomonitoring techniques to measure heavy metal exposure in free ranging birds using eggs, feathers and guano are problematic because essential metals copper (Cu) and zinc (Zn) deposited in eggs and feathers are under physiological control, feathers accumulate metals from surface contamination and guano may contain faecal metals of mixed bioavailability. This paper reports a new technique of measuring lead (Pb), Cu and Zn in avian urate spheres (AUS), the solid component of avian urine. These metal levels in AUS (theoretically representing the level of metal taken into the bloodstream, i.e. bioavailable to birds) were compared with levels in eggs (yolk and shell), feathers and whole guano from chickens (Gallus gallus domesticus) exposed to a heavy metal-contaminated soil (an allotment soil containing Pb 555?mg?kg^?1 dry mass (dm), Cu 273?mg?kg^?1?dm and Zn 827?mg?kg^?1?dm). The median metal levels (n?=?2) in AUS from chickens exposed to this contaminated soil were Pb 208???g?g^?1 uric acid, Cu 66???g?g^?1 uric acid and Zn: 526???g?g^?1 uric acid. Lead concentrations in egg yolk and shell samples (n?=?3) were below the limit of detection (<2?mg?kg^?1), while Cu and Zn were only consistently detected in the yolk, with median values of 3 and 70?mg?kg^?1 (dm), respectively, restricting the usefulness of eggs as a biomonitor. Feathers (n?=?4) had median Pb, Cu and Zn levels respectively of 15, 10 and 140?mg?kg^?1 (dm), while whole guano samples (n?=?6) were 140, 70 and 230?mg?kg^?1 (dm). Control samples were collected from another chicken flock; however, because they had no access to soil and their diet was significantly higher in Cu and Zn, no meaningful comparison was possible. Six months after site remediation, by top soil replacement, the exposed chickens had median Pb, Cu and Zn levels respectively in whole guano (n?=?6) of 30, 20 and 103?mg?kg^?1 (dm) and in AUS (n?=?4) of 147, 16 and 85???g?g^?1 uric acid. We suggest the persistent high Pb level in AUS was a consequence of bone mobilised for egg production, releasing chronically sequestered Pb deposits into the bloodstream. In contrast, AUS levels of Cu and Zn (metals under homeostatic control and sparingly stored) had declined, reflecting the lower current exposure. However because pre- and post-remediation samples were measured using different methods carried out at different laboratories, such comparisons should be guarded. The present study showed that metals can be measured in AUS, but no assessment could be made of availability or uptake to the birds because tissue and blood samples were not concomitantly analysed. A major short coming of the study was the inappropriate control group, having no access to uncontaminated soil and being fed a different diet to the exposed birds. Furthermore guano and urine analysis should have been carried out on samples from individual birds, so biological (rather than just technical) variation of metal levels could have been determined. Future studies into using AUS for biomonitoring environmental heavy metals must resolve such experimental design issues.     

Lowland Rice Response to Thermophosphate Fertilization

Abstract

Use of adequate rates of phosphorus (P) in crop production on high‐P‐fixing acid soils is essential because of high crop response to P fertilization and the high cost of P fertilizers. Information on lowland rice response to thermophosphate fertilization grown on Inceptisols is limited, and data are also lacking for soil‐test‐based P fertilization recommendations for this crop. The objective of this study was to evaluate response of lowland rice to added thermophosphate and to calibrate P soil testing for making P fertilizer recommendations. A field experiment was conducted for two consecutive years in central Brazil on a Haplaquept Inceptisol. The broadcast P rates used were 0, 131, 262, 393, 524, and 655 kg P ha^?1, applied as thermophosphate Yoorin. Rice yield and yield components were significantly increased with the application of P fertilizer. Average maximum grain yield was obtained with the application of 509 kg P ha^?1. Uptake of macro‐ and micronutrients had significant quadratic responses with increasing P rates. Application of thermophosphate significantly decreased soil acidity and created favorable macro‐ and micronutrient environment for lowland rice growth. Across 2 years, soil‐test levels of Mehlich 1–extractable P were categorized, based on relative grain yield, as very low (0–17 mg P kg^?1 soil), low (17–32 mg P kg^?1 soil), medium (32–45 mg P kg^?1 soil), or high (>45 mg P kg^?1 soil). Similarly, soil‐test levels of Bray 1–extractable P across 2 years were very low (0–17 mg P kg^?1 soil), low (17–28 mg P kg^?1 soil), medium (28–35 mg P kg^?1 soil), or high (>35 mg P kg^?1 soil). Soil P availability indices for Mehlich 1 extractant were slightly higher at higher P rates. However, both the extracting solutions had highly significant association with grain yield.     

Effect of Sheep Manure and Its Produced Vermicompost and Biochar on the Properties of a Calcareous Soil after Barley Harvest

ABSTRACT

Conversion of manures to vermicompost and biochar may alleviate some negative effects of manure application to soil but the efficiency of the produced vermicompost and biochar as compared to their feedstocks is not well-known. In the current investigation, we compared the effects of sheep manure and its derived vermicompost and biochar (pyrolyzed at 400°C for 4 h) on the properties of a calcareous soil that planted with five cultivars of barley (Behrokh, Khatam, Reyhaneh03, Fajr 30 and Nimrooz) for 60 days. Different soil properties and availability of nutrients and barley yield were determined after plant harvest. The biochar significantly increased barley yield rather than control (4.20 vs. 3.57 g pot^?1), but sheep manure and vermicompost had no effect on it (3.51 and 3.37 g pot^?1, respectively). Fajr 30 and Nimrooz (3.52 and 3.42 g pot^?1, respectively) had significantly lower yield than other cultivars. Biochar increased soil pH up to 8.2. Soil salinity was increased by application of all organic materials (increase to 16–36%). Cation exchange capacity (CEC) and organic matter content of soil were also increased by all organic materials application (0.4–0.9 cmol kg^?1 and 0.33–0.50%, respectively). All organic materials increased total nitrogen (N), but this increase was the highest with sheep manure application (53%). The availability of phosphorus (P) and potassium (K) was increased significantly by application of all organic materials, and this increase was the highest with biochar application (19 and 309 mg kg^?1, respectively). Biochar application had no effect on the availability of micronutrients, but application of sheep manure and vermicompost increased the availability of iron (Fe) (0.62 and 0.48 mg kg^?1, respectively) and zinc (Zn) (0.18 and 0.37 mg kg^?1, respectively). Generally, organic materials may change the status of soil nutrients via change in soil pH, organic matter content, release of nutrients, increase in soil CEC and formation of soluble complex with nutrients.     

Influence of Spent Mushroom Compost (SMC) as an Organic Fertilizer on Nutrient,Growth, Yield,and Essential Oil Composition of German Chamomile (Matricaria Recutita L.)

To investigate the effect of Spent Mushroom Compost (SMC) as an organic fertilizer on German chamomile (Matricaria recutita L.) nutrient, growth, yield, essential oil and osmolytes a greenhouse experiment was conducted through a randomized complete design in six replications. A mixture of sandy loam soil with 5, 10 and 15% volume rates of spent mushroom compost was used as the partial substrate for German chamomile pot culture. Finding of results revealed that usage of SMC in the growing media increased significantly plant growth, flower yield, essential macro nutrient uptake, sodium concentration, proline and soluble sugars content as well as essential oil percentages in compared to control. Comparison between the treatments indicated that addition of 10% SMC to the plant soil substrate cause to higher growth and addition of higher rates of SMC (10% and 15%) led to better yield. The obtained results showed that absorption of K and Na enhanced significantly by increasing of SMC percentage in growing media but there was no significant difference in N and P uptake in SMC treatments. The results of GC and GC-MS analysis presented that the main compositions of essential oil extracted from German chamomile flowers accounted for 83.99–99.84% of total essential oil in SMC treatments. Regarding important role of SMC on the essential oil yield, we can consider SMC could be a suitable substitute for chemical fertilizers as environmentally friendly material in cultivation medicinal plant German chamomile.     

Interaction matters: Synergy between vermicompost and PGPR agents improves soil quality,crop quality and crop yield in the field

Organic amendments not only promote soil quality and plant performance directly but also facilitate the establishment of introduced microbial agents. A field experiment with a fully factorial design was conducted using three levels of vermicompost (without vermicompost, low dose of 15 Mg ha⁻¹ and high dose of 30 Mg ha⁻¹), with and without plant growth-promoting rhizobacteria (PGPR) to investigate their effects in a tomato – by spinach rotation system. Our results demonstrated that applying PGPR alone had no effect on soil properties and crop performance. Vermicompost enhanced the beneficial effects of PGPR on both soil and crop, with the extent of promotion depending on the dose of vermicompost and crop types. In the presence of vermicompost, PGPR significantly (P < 0.05) reduced soil carbon and nitrogen but increased soil microbial biomass carbon and nitrogen. PGPR also significantly increased the yield of tomato and spinach under the low dose of vermicompost, but only significantly increased tomato yield under the high dose of vermicompost. There were strongly synergistic effects between vermicompost and PGPR on crop quality, with crop nitrate concentration being significantly decreased, while the vitamin C in tomato and soluble protein in spinach was significantly increased. Our results revealed the high potential of integrating vermicompost and microbial agents to substitute for regular chemical fertilization practices.     

The influence of phosphate fertilizer application levels and cultivars on cadmium uptake by Komatsuna (Brassica rapa L. var. perviridis)

Cadmium (Cd) is a common impurity in phosphate fertilizers and application of phosphate fertilizer may contribute to soil Cd accumulation. Changes in Cd burdens to agricultural soils and the potential for plant Cd accumulation resulting from fertilizer input were investigated in this study. A field experiment was conducted on Haplaquept to investigate the influence of calcium superphosphate on extractable and total soil Cd and on growth and Cd uptake of different Komatsuna (Brassica rapa L. var. perviridis) cultivars. Four cultivars of Komatsuna were grown on the soil and harvested after 60 days. The superphosphate application increased total soil Cd from 2.51 to 2.75?mg?kg^?1, 0.1?mol?L^?1 hydrochloric acid (HCl) extractable Cd from 1.48 to 1.55?mg?kg^?1, 0.01?mol?L^?1 HCl extractable Cd from 0.043 to 0.046?mg?kg^?1 and water extractable Cd from 0.0057 to 0.0077?mg?kg^?1. Cd input reached 5.68?g?ha^–1 at a rate of 240?kg?ha^–1 superphosphate fertilizer application. Superphosphate affected dry-matter yield of leaves to different degrees in each cultivar. ‘Nakamachi’ produced the highest yield in 2008 and ‘Hamami No. 2’ in 2009. Compared with the control (no phosphate fertilizer), application of superphosphate at a rate of 240?kg?ha^–1 increased the Cd concentration in dry leaves by 0.14?mg?kg^?1 in ‘Maruha’, 1.03?mg?kg^?1 in ‘Nakamachi’, 0.63?mg?kg^?1 in ‘SC8-007’ in 2008, and by 0.19?mg?kg^?1 in Maruha’, 0.17?mg?kg^?1 in ‘Hamami No. 2’, while it decreased by 0.27?mg?kg^?1 in ‘Nakamachi’ in 2009. Field experiments in two years demonstrated that applications of different levels of calcium superphosphate did not influence Cd concentration in soil and Komatsuna significantly. However, there was a significant difference in Cd concentration of fresh and dry Komatsuna leaves among four cultivars in 2008 and 2009. The highest Cd concentration was found in the ‘Nakamachi’ cultivar (2.14?mg?kg^?1 in 2008 and 1.91?mg?kg^?1 in 2009). The lowest Cd concentration was observed in the ‘Maruha’ cultivar (1.51?mg?kg^?1?dry weight (DW)) in 2008 and in the ‘Hamami No. 2’ cultivar (1.56?mg?kg^?1?DW) in 2009. A decreasing trend in Cd concentration was found in ‘Nakamachi’, followed by ‘SC8-007’, ‘Hamami No. 2’ and ‘Maruha’ successively. It is necessary to consider a low-uptake cultivar for growing in a Cd polluted soil. In these two years’ results, ‘Maruha’ cultivar was the lowest Cd uptake cultivar compared to the others.     

Integrated use of organic and inorganic inputs in wheat-fodder maize cropping sequence to improve crop yields and soil properties

A 2-year field study was conducted to evaluate the effect of two organics, farmyard manure and vermicompost, each at three rates (0, 5, 10 t ha^?1 and 0, 1, 2 t ha^?1, respectively), along with two levels of mineral fertilizer (75% and 100% of recommended dose), on crops yields and soil properties under a wheat–fodder maize cropping sequence. Individual addition of organics at a higher level increased yields of wheat and subsequent maize. Soil microbial biomass carbon was enhanced as both a direct and residual effect with the addition of farmyard manure followed by vermicompost and fertilizer treatments, and also by combined addition of manure with either vermicompost or mineral fertilizer. Farmyard manure increased the availability of soil macro- and micronutrients, whereas vermicompost influenced only the availability of micronutrients at wheat harvest. A residual effect of farmyard manure and mineral fertilizers was found for available N. Meanwhile, the residual status of micronutrients in the soil was either maintained or significantly improved due to organic amendments (Mn and Zn with farmyard manure; Fe and Zn with vermicompost). Interaction of farmyard manure and vermicompost at a higher level benefited the next crop by increasing the yield of fodder maize and improving the availability of P and metals in soil.     

Schwermetallanreicherung und Schwermetallverfügbarkeit im Einsickerungsbereich von Stammablaufwasser in Buchenwäldern (Fagus sylvatica) des Wienerwaldes

Heavy metal enrichment and mobility in the infiltration zone of stemflow from beech (Fagus sylvatica) in the Vienna woods Filtering action of beech crowns in a polluted atmosphere produces acidic stemflow enriched with heavy metals. Series of soil profiles through the infiltration zone of stemflow waters were analyzed in three old growth beech stands on pseudogley (aqualf) soils of the northeastern Vienna Woods to study soil acidification and heavy metal accumulation and mobility: + In the infiltration zone of stemflow soil pH can be less than pH 3 and thus more than 2 pH units lower than in profiles more distant from the stem base. + Heavy metal contents of the soil peaked near the stembase. Maximum values for lead were 500 to 1.200 mg·kg^?1 soil, for copper 70 to 150 mg·kg^?1 soil and for zinc 250 to 500 mg·kg^?1 soil. + Manganese was not enriched but leached out from the soil profile. + The proportion of AED-complexable lead and copper to total lead and copper decreased under stemflow influence in the topsoil but increased in the subsoil. These results indicate that significant amounts of heavy metals are deposited with stemflow in beech stands of the Vienna Woods. Strong soil acidification enhances heavy metal solubility and thus promotes leaching into deeper soil strata.     

Microbial Biomass and Tolerance of Microbial Community on an Aged Heavy Metal Polluted Floodplain in Japan

The objective of the present study was to increase understanding of the effects of heavy metal pollution and soil properties on microorganisms in relation to the biomass and microbial functional community. Soil samples were collected from aged polluted and reference sites on a floodplain. The soil Cu, Zn and Pb total concentrations were much higher at the polluted sites (average 231.6–309.9 mg kg^?1, 195.7–233.0 mg kg^?1, and 72.4–86.0 mg kg^?1, respectively) than at the reference site (average 33.3–44.0 mg kg^?1, 76.7–98.0 mg kg^?1, and 30.8–41.6 mg kg^?1, respectively), while the available heavy metal concentrations in CaCl₂ extraction were similar in all sites. Small seasonal variations in the size of microbial biomass were observed. Ambient soil properties (e.g. total C, N, pH, moisture content, and CEC) affected the soil microbial biomass more than the heavy metal pollution. However, the aged pollution tended to impact on the composition of the microbial community. PICT (pollution-induced community tolerance) test using BIOLOG Ecoplates showed enhanced tolerance of the microbial community to Cu stress in the polluted site. In non polluted but low nutrient, low pH and low moisture soil, the microbial biomass was lower and the microbial community was more vulnerable to Cu stress. In spite of the low heavy metal availability due to ageing, the BIOLOG technique provided sensitive detection of microbial community level changes in PICT analysis.     

BORON AND LIME EFFECTS ON YIELD AND DEFICIENCY SYMPTOMS OF RICE GROWN IN GREENHOUSE ON ACID TYPIC GLOSSAQUALF*

ABSTRACT

No boron (B) deficiencies have been reported for rice (Oryza sativa L.) grown in the United States and, when occurring elsewhere, reports often lack details of deficiency symptoms and leaf-B critical values. An experiment was conducted to determine the effect of B and lime on yield, pollen viability, and to determine diagnostic symptoms of B deficiency in rice. Rice cv. “Bengal” was grown in the greenhouse on a soil acquired from a rice farm from SW Louisiana in the United States, a Caddo silt loam (Caddo sl) (Typic Glossaqualf, fine-silty, siliceous, thermic), treated with 0.44?mg?B?kg^?1 (+B) or no B (?B). Split plots were limed at rates of (i) none; (ii) 224?mg?kg^?1 CaO+40.3?mg?kg^?1 MgO; and (iii) 673?mg?kg^?1 CaO+121?mg?kg^?1 MgO. Rice was also grown in ?B and +B potting media and in ?B sand culture using nutrient solution identical to that used in other studies of ours. Rough-rice yields from the +B Caddo sl treatment was 11% higher than from the ?B treatment (29.3 vs. 26.3?g?pot^?1; P=0.02). The yield increase was likely due to fewer damaged pollen (8%) found from the +B than the ?B treatment (17%; P=0.014). Leaf-B at tillering was 11.3?mg?kg^?1 for the B-treated rice and 7.1?mg?kg^?1 from the ?B treatment. Liming did not significantly affect leaf B or yields. Boron deficiency symptoms were found only in the ?B sand-culture where yields were 1.1?g?pot^?1, 96% less than that from the +B Caddo sl. Symptoms were like those found in our earlier hydroponic studies with twisted and whitish leaf tips starting at tillering and 1-cm white bands across the width of leaves. Maturity was delayed about four weeks. Boron deficiency from the Caddo sl and sand treatments occurred with leaf B≤7?mg?kg^?1 and with a Caddo sl soil B of 0.18?mg?hws (hot-water soluble) B?kg^?1. Given that no B deficiency symptoms were found in rice experiencing moderate yield loss grown on the rice soil, one must rely on soil and plant analyses to help detect likely candidates for moderate B deficiency.