Biochemical side effects of the herbicide FINALE<Superscript>®</Superscript> on <Emphasis Type="Italic">bar</Emphasis> gene-containing transgenic pineapple plantlets

Pineapple is one of the most important tropical fruits and therefore intensive genetic improvement programs are being carried out in many countries, including Cuba. Our research team has previously introduced the bar gene, along with chitinase and AP24 genes, into the pineapple genome. Herein, we report on the biochemical side effects of the herbicide FINALE^® on these transgenic plantlets during hardening. Levels of aldehydes and chlorophylls, and peroxidase activity were recorded. The transformed clone studied here, not sprayed with FINALE^®, showed the following side effects because of transgenesis only. Levels of malondialdehyde, other aldehydes, chlorophyll b, and total chlorophyll pigments decreased. The most remarkable biochemical differences between transgenic and non-transgenic plantlets after application of FINALE^® follow. Levels of malondialdehyde and other aldehydes in transgenic material were not decreased by FINALE^®, perhaps because these levels were already low as a result of transformation. FINALE^® increased peroxidase activity in transgenic plantlets but such increase was higher in non-transgenic material. The herbicide increased contents of chlorophyll pigments (a, b, total) in transformed plantlets. However, as expected, non-transgenic plantlets decreased levels of chlorophylls (a, b, total) after application of FINALE^®. The genetic transformation of pineapple with the bar gene not only conferred resistance to the herbicide FINALE^®, but also promoted other biochemical changes.     

科普须防“失真”，“失声”，“失效”

为了提高对科普工作的重视程度,依靠动员科学家推动科普事业,加强科普资源建设,创新科普活动,针对科普工作和活动经常出现“权威性不够”,“针对性不足”,“实效性不强”等问题。根据长期从事农业科普工作的实践,对科普“失真”,“失声”,“失效”现象产生的原因进行了深入思考,提出了解决这些问题的主要对策建议。     

"种大树"与"大树移植"

由于环境的不断恶化,植树绿化活动在我国城乡风起云涌,形成了热潮。由于“种大树”比栽花种草的生态效益大,所以提倡“种大树”的人越来越多。但目前却有一股“大树移植”风在一些城市劲吹,美其名曰“大树进城”于是十几、二十几厘米胸径的树成千上万地移植进城;更有甚者,将深山老林的古树也挖进城里。笔者认为风不可长。首先应该指出的是“种大树”与“大树移植”是两个不同的概念。虽然“大树移植”也是“种大树”,但提倡“种大树”并不等于提倡“大树移植”。 通常称的“大树”一般指“乔木”而言。《辞海》中“乔木”辞条释为“主干明显直立,分枝繁盛的木本     

奇人&#183;奇行&#183;奇花

这是一组大型奇石（图1—4），石中蕴含着奇人奇行的历史故事——米芾，字元章（元1051—1107年）。宋徽宗召之为书画学博士。他平素举止清狂、违世异俗．人称“米颠”。尤嗜奇石，见辄赞叹跪拜，为历史留下了“元章拜石”的典故。     

O6-0B014;B015;12;1;H;BB014

蜕?005000500010001;0006001052-54圆中的斯坦纳──莱麦斯定理推广的猜想证明魏春强安康教育学院!陕西安康7250000安康师专学报JOURNAL OF ANKANG TEACHERS COLLEGE55-56O182A002;73;H;AA002_73;魏春强000500020011000355-56斯坦纳定理推广的猜想证明黄岩陕西绥德四十铺中学!陕西绥德7180000安康师专学报JOURNAL OF ANKANG     

红花桉&#183;红千层&#183;白千层

红花桉(Eucalyptus ficifolia F.Muell)幼叶与成长叶异样，成熟叶片大，革质，卵形至倒卵形，长7～11厘米，宽4～6厘米，先端有尖头，叶柄长2～3厘米，中脉两面突起，侧脉羽状，两面明显，叶片上面深绿，具蜡质层，下面苍绿，网脉明显。花大，有花梗，花排成聚伞式圆锥花序；萼片与花瓣连合成一帽状体；种子极大，最大者长达1厘米。宽0．6厘米．     

鸢尾&#183;有髯鸢尾&#183;德国鸢尾

鸢尾类植物是花卉栽培中园艺化较早、种类丰富且在国际上久负盛名的多年生草本花卉，现今广泛应用于园林绿化、庭院种植、湿地景观及切花栽培中。 1、中国的鸢尾 鸢尾在我国古代又称乌园、乌鸢，最早见于文字的记载是公元1～2世纪成书的《神农本草经》。古代著作中常将鸢尾与射干混淆，而对射干的记载最早见于公元前苟子的《劝学篇》。     

蟹爪兰嫁接拾遗&#183;补漏&#183;纠偏

蟹爪兰嫁接的有关问题已是不少书刊谈了又谈的，但笔者认为仍有许多重要细节被遗漏甚至被误导了。     

吉祥草&#183;阔叶麦冬&#183;书带草

仲夏之夜,一阵阵清香扑鼻而来,寻香而至,眼前一片娇黄的花朵正在悄然绽放。这种开黄花的植物名叫大花萱草(Hemerocallis fulva L),为百合科萱草属多年生宿根草本植物。根为纺锤形肉质根,须根大多长在