Effect of a dental chew on dental substrates and gingivitis in cats

The use of special diets or dietary adjuncts, although not eliminating the need for regular professional therapy, may serve to improve gingival health during the interval between professional oral health assessments and intervention. This study was performed to determine the effect of a dental chew on dental hygiene and gingival health in cats. The accumulation of dental substrates and the development of gingivitis were assessed in cats fed either a dry diet only or a dry diet supplemented with dental chews. A two-period, crossover design was used with the test phase lasting 4-weeks. Results indicated that the daily addition of dental chews to a dry diet was effective in reducing plaque and calculus accumulation on tooth surfaces in cats, as well as reducing the severity of gingivitis.     

Effect on canine oral health of adding chlorhexidine to a dental hygiene chew

A study to compare the effect of a dental hygiene chew with or without 0.2% chlorhexidine on the development of gingivitis and the accumulation of dental deposits was performed using 11 small dogs. Confirming previous data, the daily addition of a standard chew to a dry diet resulted in significantly less gingivitis and calculus after 3 weeks compared with feeding the dry diet alone. Addition of chlorhexidine to the chew made no difference to the degree of gingivitis or the amount of calculus that accumulated, but did result in significantly less plaque accumulation after 3 weeks. The abrasiveness of the chew, rather than the antibacterial activity of chlorhexidine, is likely to have contributed the most to the maintenance of oral health in dogs with mild gingivitis.     

Effectiveness of a vegetable dental chew on periodontal disease parameters in toy breed dogs

Sixteen toy breed dogs completed a parallel, 70-day two-period, cross-over design clinical study to determine the effect of a vegetable dental chew on gingivitis, halitosis, plaque, and calculus accumulations. The dogs were randomly assigned into two groups. During one study period the dogs were fed a non-dental dry diet only and during the second study period were fed the same dry diet supplemented by the daily addition of a vegetable dental chew. Daily administration of the dental chew was shown to reduce halitosis, as well as, significantly reduce gingivitis, plaque and calculus accumulation and therefore may play a significant role in the improvement of canine oral health over the long-term.     

Benefits of a 'dental hygiene chew' on the periodontal health of cats

A study was undertaken to determine the clinical efficacy of a chew designed to improve dental hygiene in the cat. The accumulation of dental deposits (plaque and calculus) and the effect on gingival inflammation were assessed in 15 client-owned cats while on two different regimens. In the first leg of the study, the cats were fed a nutritionally complete dry diet supplemented with one dental hygiene chew. In the second leg of the study, the same cats were fed only the nutritionally complete diet. This study demonstrated that the daily addition of the chew to the dry diet resulted in significantly less plaque and calculus accumulation on tooth surfaces. It was our experience that daily feeding of the chew helped maintain dental hygiene in cats; however, regular professional therapy is still indicated.     

Effect of two dietary regimens on gingivitis in the dog

The effect of two commercially available dietary regimens on the development of gingivitis and the accumulation of dental plaque, calculus and stain in the dog were studied. One regimen consisted of feeding an oral hygiene chew and a standard dry diet.
The other was based on a specifically designed oral care dry main meal. The study was conducted using 15 dogs in a two period, parallel cross-over design. Each period consisted of a one week pretest phase followed by a three week test phase. During the test phase, gingivitis and plaque were measured after one week and gingivitis, calculus, stain and plaque were measured after a further two weeks. Comparison of the two regimens showed that there were equal amounts of gingivitis, plaque and calculus by the end of the study. There was significantly more stain and significant loss in bodyweight when the main meal was fed.
The role of dietary modification in the maintenance of periodontal health is discussed.     

Effective periodontal disease control using dental hygiene chews

This study evaluated the effectiveness of a newly developed dental hygiene chew for dogs, with and without a natural antimicrobial additive, compared with a reference diet. Efficacy was determined by measuring the severity of gingivitis and the accumulation of dental plaque and calculus in dogs after 4-weeks of being fed the different dietary regimens. Dogs fed a single daily dental chew had significantly less gingivitis (P = 0. 02), plaque (P = 0. 0004), and calculus (P = 0.0001) compared with dogs in the control group that were fed an identical diet but received no chews. The inclusion of the antimicrobial agent did not improve the efficacy of the product. The dental hygiene chews tested in this study have potential to help reduce the incidence of periodontal disease in dogs.     

Long-term effects of a dental hygiene chew on the periodontal health of dogs

The objective of the study was to investigate the long-term effects of feeding a dental hygiene chew that has been shown to be effective in promoting periodontal health in dogs in short-term studies. Oral malodor, calculus, and plaque scores were still significantly lower after 21 months in the group that was receiving the dental hygiene chew, although gingivitis scores no longer differed significantly. There were no reports of any adverse reactions during the study. The results of the study support that feeding of the dental hygiene chew six days per week reduces accumulation of dental deposits, helps maintain periodontal health, and increases the time interval between professional periodontal intervention.     

Effectiveness of an oral hygiene chew to reduce dental deposits in small breed dogs

Over a period of 4-months, feeding small breed dogs with a regular dry diet supplemented with a daily oral hygiene chew resulted in a significant reduction in plaque deposition (17.3%) and calculus accumulation (45.8%) compared with dogs fed the dry diet alone. Daily feeding of a specifically designed oral hygiene chew that reduces plaque and calculus accumulation may play a role in the severity of canine periodontal disease.     

Effectiveness of an enzymatic rawhide dental chew to reduce plaque in beagle dogs

Tooth brushing is considered a superior technique for reducing plaque accumulation. Other methods of maintaining oral hygiene have been investigated since many owners may not be willing or able to brush their dog's teeth. Following a professional teeth cleaning procedure, 11 dogs were offered a rawhide dental chew BID for 7-days, while 11 other dogs were fed the same diet without receiving the chew device. Dogs in the treatment group had significantly less plaque formation during the trial period compared with dogs in the control group. The rawhide dental chew provided in the study reported here decreases plaque formation in the short-term and may be beneficial in the prevention of progressive periodontal disease associated with attachment loss if provided on a long-term basis.     

Effects of a dental food on plaque accumulation and gingival health in dogs

Recent studies have demonstrated that typical dry dog foods have significantly less efficacy in reducing accumulation of dental substrates compared to a specifically formulated and processed dental food. This study compared the effects of a typical dry food and a dental food on plaque accumulation and gingival inflammation in dogs during a 6-month period. Daily feeding of the dental food significantly reduced plaque and gingivitis by 39% and 36%, respectively, compared with daily feeding of the typical dry food. Feeding a food that decreases plaque accumulation and reduces gingivitis contributes to canine oral health maintenance.     

Effectiveness of feeding large kibbles with mechanical cleaning properties in cats with gingivitis

Periodontal disease is the most common acquired oral disease in cats. It starts with plaque accumulation and gingivitis. The aim of this study was to evaluate the effectiveness of different types of kibble and teeth brushing after professional dental cleaning. To this end, the ratio of facultative anaerobic and obligate anaerobic bacteria was determined, and the amount of plaque and tartar and the severity of gingivitis were measured. Eighty-eight cats with gingivitis and tartar were randomly divided into four groups and given different treatments after professional dental cleaning. The dental treatments were: group 1, cats were fed on small kibbles with mechanical cleaning qualities; group 2, cats were fed on small kibbles without mechanical cleaning qualities, and owners were instructed to brush the cats' teeth; group 3, cats were fed on large kibbles with mechanical cleaning qualities; and group 4, cats were fed on the same kibbles as group 2, but owners were not asked to brush their cats' teeth. A supragingival plaque sample was taken, and used for bacteriological analysis scores for tartar, plaque, and gingivitis were determined at the initial presentation and at 3 and 6 months after professional dental cleaning. Cats fed on the large kibbles with mechanical cleaning qualities (group 3) had significantly less gingivitis and tartar.     

Feline dental disease

Periodontal disease and chronic gingivitis/stomatitis are the most common feline dental diseases. With routine dental care and increased emphasis on home oral hygiene, these diseases can be controlled. Cats can be seen with a number of other dental disorders, and improved treatment methods such as restorations of early subgingival resorptive lesions, endodontic therapy, and orthodontic therapy can be performed successfully. More study and research are necessary about the gingivitis/stomatitis syndrome and subgingival resorptive lesions so that improved prevention and treatment recommendations can be made.     

Canine oral malodor

Canine oral malodor may be the first indication that a serious disease process is occurring in the oral cavity. Various methods for detecting oral malodor have been used in humans, and some of these are suitable for collecting data in the dog. Oral malodor often responds favorably to improved oral hygiene, administered first by a trained professional and followed with adequate home care.     

Assessment of Alloxan-Induced Diabetic Rats as a Periodontal Disease Model Using a Selective Cyclooxygenase (COX)-2 Inhibitor

Several recent studies have reported that alloxan-treated rats with long-term hyperglycemia can develop naturally occurring periodontal disease (PD). Our previous studies detected dental caries in the same model. Therefore, these two lesions of different etiologies are expected to occur concurrently. In this study, we evaluated the use of diabetic rats as a PD model by employing a selective COX-2 inhibitor reported to be effective against PD. Six-week-old female F344 rats were divided into 3 groups: intact rats (control), alloxan-induced diabetic rats fed a standard diet (AL) and alloxan-induced diabetic rats fed a diet containing 0.01% etodolac (AL+Et). The animals were euthanized at 26 weeks of age, and their oral tissues were examined histopathologically. Gingivitis, marginal periodontitis and alveolar bone resorption were markedly enhanced along with dental caries in the AL group compared with the control group. However, the COX-2 inhibitor had no effect on periodontal inflammation in the AL+Et group. In addition, in the AL group, periodontitis was notably nonexistent around the normal molars, and gingivitis was scarcely worse than that in the control group. In the diabetic rats, the progression of periodontal inflammation was closely correlated with the severity of adjacent dental caries, and marginal periodontitis was frequently continuous with apical periodontitis. In conclusion, an alloxan-induced diabetic rat is not a model of PD but of dental caries. It is probable that in this model, hyperglycemia may enable crown caries to progress to apical periodontitis, while the associated inflammation may rostrally expand to surrounding periodontal tissue.     

Aetiology of food-related oral lesions in chickens

Brown Leghorn chickens fed on a mash diet developed ulcerated oral lesions with extensive epithelial erosion and large colonies of bacteria. Some birds had lesions by six weeks old and by 30 weeks oral lesions were present in all birds fed on a mash diet. These lesions occur infrequently in birds fed a pelleted diet and the relatively extensive lesions shown by birds on a mash diet heal quickly (in many cases within two weeks) when the birds are transferred to a pelleted diet. The cause of the lesion is unknown but there was no evidence for food impaction, mechanical damage to the epithelium, specific dietary constituents or blocked salivary ducts. The lesions may be due to poor oral hygiene which results from the lack of mechanical stimulation of the oral epithelium to which fine particles of mash adhere.     

Dental chews positively shift the oral microbiota of adult dogs

Microbiota plays a prominent role in periodontal disease, but the canine oral microbiota and how dental chews may affect these populations have been poorly studied. We aimed to determine the differences in oral microbiota of adult dogs consuming dental chews compared with control dogs consuming only a diet. Twelve adult female beagle dogs (mean age = 5.31 ± 1.08 yr) were used in a replicated 4 × 4 Latin square design consisting of 28-d periods. Treatments (n = 12/group) included: diet only (CT); diet + Bones & Chews Dental Treats (BC; Chewy, Inc., Dania Beach, FL); diet + Dr. Lyon’s Grain-Free Dental Treats (DL; Dr. Lyon’s, LLC, Dania Beach, FL); and diet + Greenies Dental Treats (GR; Mars Petcare US, Franklin, TN). Each day, one chew was provided 4 h after mealtime. On day 27, breath samples were analyzed for total volatile sulfur compound concentrations using a Halimeter. On day 0 of each period, teeth were cleaned by a veterinary dentist blinded to treatments. Teeth were scored for plaque, calculus, and gingivitis by the same veterinary dentist on day 28 of each period. After scoring, salivary (SAL), subgingival (SUB), and supragingival (SUP) samples were collected for microbiota analysis using Illumina MiSeq. All data were analyzed using SAS (version 9.4) using the Mixed Models procedure, with P < 0.05 considered significant. All dogs consuming chews had lower calculus coverage and thickness, pocket depth and bleeding, plaque thickness, and halitosis compared with CT. In all sites of collection, CT dogs had a higher relative abundance of one or more potentially pathogenic bacteria (Porphyromonas, Anaerovorax, Desulfomicrobium, Tannerella, and Treponema) and lower relative abundance of one or more genera associated with oral health (Neisseria, Corynebacterium, Capnocytophaga, Actinomyces, Lautropia, Bergeyella, and Moraxella) than those fed chews. DL reduced Porphyromonas in SUP and SUB samples. DL and GR reduced Treponema in SUP samples. DL increased Corynebacterium in all sites of collection. BC increased Corynebacterium in SAL samples. DL and GR increased Neisseria in SAL samples. DL increased Actinomyces in the SUB sample. GR increased Actinomyces in SAL samples. Our results suggest that the dental chews tested in this study may aid in reducing periodontal disease risk in dogs by beneficially shifting the microbiota inhabiting plaque and saliva of a dog’s oral cavity. These shifts occurred over a short period of time and were correlated with improved oral health scores.     

Review of studies assessing plaque accumulation and gingival inflammation in dogs

Periodontal disease is difficult to measure objectively. Many indices measuring plaque accumulation and gingivitis have been designed for humans, the Silness and L?e plaque index and Turesky modification of the Quigley and Hein plaque index being examples of well-accepted systems. It may, however, be beneficial to consider new or modified measurement systems for dogs, and such veterinary modifications need to be supported and clearly identified. This article reviews the origins of clinical periodontal indices now in common use in studies that examine the effectiveness of oral hygiene products.     

Effect of in-water iodine supplementation on weight gain, diarrhea and oral and dental health of nursery pigs

A farm trial was conducted to evaluate the effect of in-water iodine on piglet growth, the incidence of diarrhea, and the development of deleterious oral and dental conditions. A total of 208 weaned piglets were included in the study. Piglets were weighed 3 times: within 24 h of weaning, and 3 wk and 6 wk after weaning. A concentration of 1 ppm iodine was provided in their drinking water. Swabs were taken from all water nipples and water lines and pooled fecal samples were collected from all pen floors. Fecal samples were also collected from sows at weaning. The swabs and fecal samples were tested for the presence of Salmonella and Escherichia coli. Within 24 h of each weighing, a complete oral examination was performed on each piglet. No significant difference in growth (P > 0.05) or dental conditions (P > 0.05) was found among treatment groups during the period that iodine was added to the drinking water. After weaning, all deleterious oral conditions increased (oral lesions from weaning to 6 wk, staining and caries from weaning to 3 wk, gingivitis from 3 wk to 6 wk; P < 0.05). Only gingivitis was found to be negatively associated with piglet weight (P < 0.05). Salmonella was cultured only twice from fecal samples and never from water nipples. Only 1 sow tested positive for Salmonella and E. coli O139: K82 and O157:K"V17 were cultured only rarely from the water nipples. No signs of diarrhea were noted throughout the study. Adding an aqueous iodine supplement to nursery pigs, therefore, did not provide an advantage for either growth or oral condition. Deleterious oral conditions do increase after weaning, with gingivitis being associated with lower piglet weight.     

Gingivitis/stomatitis in cats.

Any alteration in the balance of bacterial challenge versus the host's ability to resist and repair will result in oral lesions that are similar in appearance. The bacterial cause of gingivitis and periodontitis in humans and in all other animals in which it has been studied is firmly established, and specific species of predominantly gram-negative anaerobes have been implicated. Naturally occurring or acquired immunopathologies are likely to result in premature dental disease. When oral disease is associated with the accumulation of plaque, a positive response can be achieved by reducing the bacterial challenge to the host through the maintenance of oral hygiene by timely professional dental prophylaxis and home care. Disease that is the result of atypical immune responses, however, can be much more difficult to manage. Such oral disease can occur with either immune deficiencies or exaggerated immune responses, and it is likely that multiple mechanisms are active concurrently. In any case, gram-negative anaerobes present in plaque are likely to be a major contributing factor. Therefore patients with chronic refractory gingivitis-stomatitis must be considered to be plaque intolerant. Only with a frequent regimen of aggressive and thorough professional dental treatment plus meticulous oral home care on a daily basis can one expect to keep these cases in remission. Because this is often unrealistic, the only other way to keep these patients free of disease is by total dental extraction. The tissues that are colonized by the causative organisms must be eliminated. All root tips and bony sequestra must be removed and healing with intact epithelium accomplished before these cases will go into remission. Edentulous feline patients that continue to have signs of gingivostomatitis have been found to have an area of nonhealed bony sequestrum and chronic osteomyelitis. Once effective debridement has been accomplished and epithelial healing completed, nonresponsive cases can be expected to go into remission (Color Plate 2, Figure 7). It is hoped that as more is learned about this frustrating problem, the many factors influencing feline oral disease will be scientifically documented. In the future, actual diagnoses can be systematically made early on in disease, and treatment will be more than just symptomatic.     

Feline struvite urolithiasis: factors affecting urine pH may be more important than magnesium levels in food

Eighteen male cats were fed either a canned complete diet or a commercially available dry pelleted diet or the same dry diet containing 1.6 per cent ammonium chloride. The daily food and water intake of four of these cats was measured. Urine samples were taken at random and the pH and the presence of struvite crystals in their sediment estimated. In some samples in which the pH was less than 7.0, and struvite crystals were absent, the pH was increased to 7.0 and any sediment was examined for struvite. No spontaneous struvite was seen in urine samples (pH 5.8) from cats fed the canned complete diet but when its pH was raised to 7.0, 46 per cent of these samples showed struvite. Cats fed the dry pelleted diet had urine of higher pH (7.55) and 78 per cent of the samples contained struvite crystals. Cats fed this diet supplemented with ammonium chloride had a urine pH of 5.97 and only 9 per cent contained struvite crystals but when the pH was adjusted to 7.0 all the samples showed struvite crystals. Energy intake was similar on all three diets but the intake of dry matter was greater on the dry diets. Liquid water intake was greater on the dry diets but total water intake was greatest on the canned complete diet. The intake of magnesium, calcium and phosphate was greater on the dry diets. It is concluded that urine pH is a more important controller of struvite precipitation than mineral intake.