Effects of type of forage fed to dairy heifers: associations between claw characteristics, clinical lameness, environment and behaviour

Heifers were reared separately on wet fermented (WF) (grass silage based) or dry unfermented (DU) (straw based) diets. Clinical lameness was recorded and physical claw attributes were measured regularly, up to six months after calving at approximately two years of age. Two months before calving, some aspects of animal behaviour and the properties of slurry were studied. The incidence of lameness due to claw horn lesions was significantly higher (P < 0.01) in WF than in DU. WF suffered more serious heel erosion prior to calving (P < 0.05) and had softer claw horn at two (heel and axial sole) out of five sites throughout (P < 0.01 and P < 0.05). The slurry produced by WF had lower total solids content (P < 0.05) and viscosity (P < 0.001). WF spent longer standing and feeding (P < 0.001). It is proposed that standing for longer in less viscous slurry contributed to softer claw horn and more severe heel erosion, predisposing WF to claw horn lesions causing lameness. Reducing contact with low dry matter slurry is recommended for improving claw health.     

Effect of forage type on claw horn lesion development in dairy heifers

In a continuous design study the claw health of 54 Holstein-Friesian heifer calves was recorded from three months of age until six months after first calving (30 months of age). Pre-calving heifers were either fed a wet, fermented grass silage-based diet (WF) or a dry, unfermented straw and concentrate based diet (DU), apart from grazing during their first summer. Approximately one month before calving both groups were fed a silage-based diet and afterwards all received a silage and concentrate lactation ration. Claws were examined four times during rearing, once pre-calving, and four times during lactation. Both white line and sole lesions were significantly worse for WF than DU both during rearing and throughout first lactation although the effect was not as consistent over time for white line lesions. It is concluded that for optimal claw health youngstock diets should not be heavily based on wet grass silage (less than 25% DM).     

Effects of diet concentrate level and sodium bicarbonate on site and extent of forage fiber digestion in the gastrointestinal tract of wethers

Four adult wethers (45 kg) with permanent ruminal and abomasal cannulae were used in a repeated measures Latin-square arrangement of treatments to quantitate the effects of diet concentrate level and sodium bicarbonate (NaHCO3) on site and extent of forage fiber digestion in the gastrointestinal tract. Experimental diets consisted of Kentucky-31 tall fescue hay, soybean meal and a semi-purified concentrate mixture in ratios of 95:5:0, 76:4:20, 57:3:40 and 38:2:60; NaHCO3 represented 0 or 7.5% of the concentrate mixture. Ruminal digestion (% of intake) of neutral detergent fiber (NDF) and hemicellulose decreased linearly (P less than .05), whereas acid detergent fiber (ADF) digestion responded in a cubic (P less than .05) fashion to increasing concentrate level; NaHCO3 improved ruminal digestion of NDF (P less than .10) and ADF (P less than .05), but not hemicellulose. Post-ruminal digestion (% of rumen non-degraded) of NDF and ADF tended to increase, whereas hemicellulose digestion responded in a cubic (P less than .05) fashion to increasing concentrate level; NaHCO3 decreased (P less than .05) post-ruminal digestion of all fiber fractions. Total tract digestion of NDF and ADF showed a cubic (P less than .05) response, whereas hemicellulose digestion responded in a quadratic (P less than .05) fashion to increasing concentrate level; NaHCO3 had no effect on total tract digestion of any fiber fraction. Correlations of ruminal hemicellulose digestion with mean pH (r = .33; P = .07) and minimum pH (r = .30; P = .09) were attained in a 24-h feeding cycle.(ABSTRACT TRUNCATED AT 250 WORDS)     

Voluntary feed intake, acid-base balance and partitioning of urinary nitrogen in lambs fed corn silage with added sodium bicarbonate or sodium sesquicarbonate

An experiment with growing lambs was designed to test the hypothesis that alterations in blood acid-base status would influence intake of corn silage. Six wethers (29 kg) were fed a diet of corn silage (36% DM, 8% CP) supplemented with 1.25% urea and .2% sulfur. At feeding time, sodium bicarbonate (NaHCO3) and sodium sesquicarbonate (NaSC) were added to the silage at levels of 0, 2% or 4% of diet DM. The treatments were arranged as a 2 x 3 factorial, and the study was conducted as a 6 x 4 incomplete latin square with four 17-d periods. Voluntary intake of OM was not different (P greater than .05) between NaHCO3 (1,008 g/d) and NaSC (1,041 g/d). There was no significant interaction between type of buffer (NaHCO3 or NaSC) and level of buffer on any of the variables measured. The progressive increase in buffer load did not alter feed intake (P greater than .05), although there was a quadratic response (P less than .05) in urine pH and a linear increase (P less than .01) in blood HCO3- 2 h after feeding. There was no evidence that lambs fed corn silage experienced metabolic acid stress. Urinary excretion of ammonia and urea were indicative of changes, although not pronounced, in ammoniuria and ureapoiesis in response to bicarbonate loading. This study implies that corn silage imposes no "acid stress" on lambs and, consequently, that there is no nutritional benefit in adding buffers to corn silage for sheep.     

Rumen fermentation pattern of dairy heifers fed restricted amounts of low, medium, and high concentrate diets without and with yeast culture

Restricted feeding and high concentrate diets are potential strategies for growing dairy heifers. Ruminal manipulation with additives such as Saccharomyces cerevisiae yeast culture (YC) has been shown to alter digestibility when added to this type of diet. An experiment was conducted to investigate the ruminal fermentation and in situ digestibility of diets with 3 different levels of forage to concentrate (F:C) fed at restricted intake without and with YC addition. Three cannulated post-pubertal Holstein heifers (age 18.0 ± 1.2 months; body weight 449.6 ± 19.7 kg) were fed diets consisting of corn silage as the sole forage source in a 3 period (35-day) Latin square design. Heifers were fed diets for 21 days with no YC addition, followed by 14 days where YC was added to the diet (1 g/kg as fed basis). Low (LC), medium (MC), and high (HC) concentrate diets (20, 40, and 60% concentrate) were fed once daily on a restricted basis to provide 0.22 Mcal ME/kg empty BW^0.75. Rumen fluid was sampled on days 18 and 32 of each period, and rumen contents were evacuated on days 21 and 35 of each period. An in situ study was done on days 14 to 17 and on days 28 to 31. Mean ruminal pH was not different between dietary treatments and no YC effect was detected. Mean total volatile fatty acids (VFA) and ruminal ammonia-nitrogen (NH₃-N) concentration was also not different among diets with different F:C. Molar proportions of acetate were decreased, and propionate were increased; while the acetate-to-propionate ratio was decreased as the concentrate level increased from LC to HC. Total VFA, propionate, and acetate as well as isoacids concentration increased, yet NH₃-N concentration decreased with YC addition in all diets. From these results we conclude that feeding HC diets in restricted amounts had minimal effects on rumen fermentation rate between different F:C diets. The addition of YC modified NH₃-N and volatile fatty acid concentrations in the rumen in all 3 diets in this study, presumably through alterations in end-product production and utilization.     

Effect of sodium bicarbonate and sodium bentonite on digestion, solid and liquid flow, and ruminal fermentation characteristics of forage sorghum silage-based diets fed to steers

Six ruminally cannulated steers, five Holsteins and one Hereford (250 to 295 kg), were fed 84% forage sorghum silage plus 16% supplement or 50% forage sorghum silage plus concentrate and supplement diets containing either no addition (controls), 1% sodium bicarbonate (NaHCO3) or 2% sodium bentonite in a 2 X 3 factorial arrangement of treatments in a 6 X 6 Latin-square experiment with 3-wk periods. Sodium bicarbonate increased dry matter (DM) intake when concentrate was included, but neither compound affected intake of the 84% silage diet. Bentonite lowered DM, neutral detergent fiber (NDF), and acid detergent fiber (ADF) digestibilities, but NDF disappearance from nylon bags was unchanged. Ruminal pH, osmolality and L(+) and D(-) lactate were not affected by treatment. Both NaHCO3 and bentonite tended to lower ruminal NH3-N concentrations. Bentonite lowered the molar proportion of isobutyrate in ruminal fluid relative to controls, but proportions of other volatile fatty acids (VFA) and total VFA concentrations were unchanged. Neither NaHCO3 nor bentonite affected ruminal liquid or solid volumes, dilution rate constants or ruminal outflow rates. Markers overestimated volumes, but correction with measured volumes did not change interpretation of treatment effects. It was concluded that control diets had sufficiently high baseline values of pH, dilution rate and acetate proportion to preclude changes induced by either compound, especially at 1 or 2% of DM intake. An effect on palatibility through neutralization of silage acids may have been responsible for the intake response to NaHCO3.     

日粮精粗比对奶牛瘤胃内环境的影响

本文主要讨论了日粮精粗比对奶牛瘤胃内环境的影响,包括对瘤胃细菌菌群数量、瘤胃pH值、瘤胃内容物稀释率和外流速度、氨态氮浓度以及挥发性脂肪酸的影响。     

The effect of concentrate level and concentrate composition on the performance of growing dairy heifers reared and finished for beef production

Abstract

The objectives of the study with growing dairy heifers were to determine the effects on animal performance of (1) concentrate level and (2) the concentrate composition in the grass silage-based diet. Animals (30 heifers) were randomly assigned to three feeding treatments: (1) low level (1.75 kg dry matter (DM)/d) of rolled barley (LB); (2) low level (1.75 kg DM/d) of commercial pelleted concentrate (LC); and (3) medium level (3.5 kg DM/d) of commercial pelleted concentrate (MC). All animals were also offered grass silage and hay. The commercial concentrate contained more crude protein than barley grain (135 g/kg DM vs. 205 g/kg DM). The data were analysed using analysis of variance and differences between the treatments were tested by orthogonal contrasts: LC vs. MC and LB vs. LC. The growth of the heifers increased and carcass conformation improved with increasing concentrate level. Commercial concentrate did not improve animal performance compared with barley grain.     

Minimum length of the adaptation and collection period in digestibility trials with sheep fed ad libitum only forage or forage plus concentrate

Two in vivo digestibility trials with sheep were conducted to identify the minimum period length of feeding a new diet to obtain reproducible values of nutritional variables onward and the minimum length of collection period as to obtain maximal precision for each variable. Trial 1 was conducted with ten Polwarth male sheep (34 ± 5 kg body weight (BW)) throughout three 21‐day periods, in a completely randomized two‐way crossover design. The animals were divided into two groups (Group A and B, n = 5 per group) which were fed ad libitum with a sequence of the following diets throughout the periods: Group A: hay – hay plus concentrate – hay; Group B: hay plus concentrate – hay – hay plus concentrate. The concentrate was included in a proportion of 0.33 of the total diet. The intake, and the faecal and urinary excretion were measured daily throughout the experiment. For evaluating rumen fermentation variables, in Trial 2 four Santa Inês male sheep (65 ± 5 kg BW) fitted with ruminal cannula were used. The animals were randomly divided into two groups (n = 2 per group), and the trial was conducted through four 21 days experimental period, in a three‐way crossover design, using experimental diets and feeding management similar to Trial 1. The results indicated that, even though no clear or consistent steady‐state condition was identified for rumen fermentation or urinary excretion variables, the adaptation period for measuring OM digestibility in in vivo trials with sheep fed ad libitum where the diet shifts from one of only hay to another containing concentrate, or vice‐versa, should be at least 12 days long. Moreover, although no precision improvement was obtained by increasing the collection period above 1 day for measuring OM digestibility, the minimal length of collection period should be 4 days for measuring faecal excretion variables and 7 days for measuring urinary excretion variables.     

日粮添加碳酸氢钠对荷斯坦奶牛生产性能的影响

选择体重、胎次、泌乳期、乳脂率和产奶量基本一致的健康无病的中国荷斯坦奶牛30头,随机分成3组,分别为试验组Ⅰ,Ⅱ和对照组。试验组Ⅰ,Ⅱ奶牛日粮每日每头分别添加130、150g碳酸氢钠,对照组奶牛日粮每日每头分别添加110g碳酸氢钠,试验期28d。结果表明,试验组Ⅰ干物质采食量、粪便pH比对照组高1.41%、0.20(P0.05),非脂乳固体比对照组低0.4(P0.05);试验组Ⅱ干物质采食量、产奶量、4%标准乳、粪便pH分别比对照组高2.04%、2.01%、2.68%、0.42(P0.05),乳脂率高0.13%(P0.05),乳蛋白、非乳脂固体分别低0.36%、0.51%(P0.05)。另外,碳酸氢钠的增加使得牛乳中体细胞数显著降低,且与乳脂肪呈显著负相关(P0.05),而与乳蛋白质呈显著正相关(P0.05)。由此可知,在每头奶牛日粮中添加150g碳酸氢钠的范围内,随着日粮中碳酸氢钠的增加,能显著改善荷斯坦奶牛的生产性能,有效预防奶牛瘤胃酸中毒。     

粗、精料干物质比例的变化对奶牛日粮成本的影响

就特定的泌乳奶牛营养需要量，设计了7种不同饲粮粗料与精料比例(F：C=82：18、70：30、65：35、60：40、55：45、50：50和40：60)的日粮配方，研究饲粮粗料与精料比例对奶牛日粮成本的影响规律。结果表明，日粮中精料用量不是越少越好，随着日粮中精料用量在一定范围内的增加，在大多数情况下，往往会降低日粮成本，但必须是以日粮原料中具有可以替代的廉价原料为基础。只有在综合考虑日粮成本、精料补充料的可加工性，以及粗料饲喂的可执行性等决定最合理的粗料与精料比例。     

Effects of Fusarium mycotoxins in rations with different concentrate proportions on serum haptoglobin and hepatocellular integrity in lactating dairy cows

It was hypothesized that long‐term intake of a diet contaminated with deoxynivalenol (DON) and differing in the proportion of concentrate might affect hepatocellular integrity and function as well as biomarkers of systemic inflammation in lactating dairy cows. In Period 1 (11 weeks), 26 lactating German Holstein cows (13 primiparous and 13 pluriparous, 31 days in milk, 522 kg body weight, on average) were divided into two groups and fed diets (50% concentrate) with (MYC, n = 12; on average 5.3 mg DON/kg DM) or without (CON, n = 14) DON contaminations. In Period 2 (16 weeks), each group was further divided into two groups to test whether elevated concentrate proportion as additional burden might enhance the toxicity of DON. The cows in MYC60 (n = 6; 4.6 mg DON/kg DM) and CON60 (n = 7) received the diet with 60% concentrate, while cows in MYC30 (n = 6; 4.4 mg DON/kg DM) and CON30 (n = 7) received the diet with 30% concentrate. Blood samples were taken in biweekly intervals for activities of aspartate amino transferase (AST), glutamate dehydrogenase (GLDH) and gamma‐glutamyl transferase as well as for concentration of total bilirubin and haptoglobin. Biopsies from liver were collected in week 27 for morphological analyses. No DON effect was found for the variables assessed in blood. The diet with 60% concentrate led to higher activities of AST and GLDH in Period 2. No morphological change was found by both light and electron microscopic analyses of liver samples. Results indicated that long‐term intake of DON‐contaminated diet over 27 weeks led to neither relevant damages of hepatocytes nor systemic inflammatory responses in lactating dairy cows, even if the dietary concentrate proportion was increased to 60%.     

Performance and meat quality traits of beef heifers fed with two levels of concentrate and ruminally undegradable protein

The effects of two levels of concentrate and ruminally undegradable protein (RUP) on performance, intake, digestibility, carcass characteristics, meat quality traits, and commercial cuts yield were assessed. Twenty crossbred heifers (240 kg average body weight) were used. At the beginning of the trial, four animals were slaughtered as reference group and the 16 remaining animals were randomly assigned to four treatments, in a 2 × 2 factorial design: two levels of concentrate (40% and 80%, dry matter (DM) basis) and two levels of RUP (48.79% and 27.19% of CP). At the end of the trial, all the animals were slaughtered. There was no interaction (P > 0.05) between concentrate and RUP levels. Dry matter intake and nutrients digestibility was not affected (P > 0.05) by RUP level. Heifers fed the highest RUP level had greater (P < 0.05) average daily gain (ADG). Concentrate level did not affect (P > 0.05) DMI and ADG. Heifers fed diets with 80% concentrate had greater intake of TDN and EE, and lower intake of NDF (P < 0.05). The digestibilities of all nutrients, except the NDF, were greater (P < 0.05) for the 80% concentrate diets. The RUP levels did not affect (P > 0.05) the carcass characteristics and carcass gain composition. Heifers fed 80% concentrate diets had larger (P < 0.05) longissimus muscle area and lower gain of bones (P < 0.05). Concentrate level did not affect (P > 0.05) the composition of carcass gain. There was no effect (P > 0.05) of RUP and concentrate levels on meat quality traits and commercial cut yields.     

TMR不同精粗比例对中产奶牛碳水化合物消化的影响

以40头中等产奶量的荷斯坦奶牛为研究对象,应用随机分组试验设计将试验动物分为Ⅰ、Ⅱ、Ⅲ和Ⅳ组,日粮精粗比例依次为50%:50%,40%:60%,35%:65%,30%:70%。研究TMR不同精粗比例对奶牛碳水化合物消化的影响,结果表明:随着粗饲料比例增加,CF、NFE、NDF和ADF表观消化率均呈先增加后降低趋势;其中试验Ⅱ组CF表观消化率显著(P<0.05)高于试验Ⅰ组,试验Ⅲ组极显著(P<0.01)高于试验Ⅰ组和Ⅳ组;试验Ⅲ组NDF表观消化率最高,并显著(P<0.05)高于试验Ⅳ组;试验Ⅱ的ADF表观消化率组较高,但各组间均差异不显著(P>0.05)。通过比较发现,当TMR日粮中粗饲料比例在60%~65%时各试验组CF、NDF、ADF和NFE表观消化率均较高,即碳水化合物消化利用最充分。因此,TMR的精粗比例为(40%:60%)~(35%:65%)是中产奶牛TMR较适宜的比例。