Effect of chelated copper sources on performance of nursery and growing pigs

Four experiments were conducted to determine the effect of Cu source and level and an antimicrobial agent on performance of nursery (6 to 25 kg) and growing (20 to 65 kg) pigs. Copper was fed either as CuSO4.5H2O (CS), inorganic chelated Cu (ICC) or organic chelated Cu (OCC) to provide 31.25 to 250 ppm supplemental Cu. In Exp. 1, 224 pigs were used to study Cu source and level added to nursery diets. No difference (P less than .05) among treatments was observed during the nursery period. Treatments were continued the first 56 d of the growing-finishing period. Regardless of the Cu source, pigs receiving 125 ppm added Cu gained faster (P less than .05) than pigs in other treatments. In Exp. 2, 216 pigs were used to determine the optimum level of CS and ICC in nursery diets. Pigs were less efficient (P less than .01) when Cu was added at 62.5 and 125 ppm than at 250 ppm (1.69, 1.72 and 1.59, feed/gain respectively). In Exp. 3, no differences (P greater than .05) in performance between sources or among levels of Cu were found. In Exp. 4, 216 pigs were utilized to determine the combined effects of Cu source and an antimicrobial on performance. Pigs fed ICC were less efficient (P less than .01) than pigs fed either OCC or CS (1.99, 1.85 and 1.90, respectively). The inorganic and organic chelated Cu compounds used in these studies were not more efficacious than CS for nursery or growing pigs.     

Influence of dietary rapeseed oil, vitamin E, and copper on the performance and the antioxidative and oxidative status of pigs.

We investigated the effects of dietary copper and vitamin E in diets containing 6% rapeseed oil on the performance and the antioxidative and oxidative status of growing pigs. The 10 dietary treatments consisted of a basal diet (9 mg of vitamin E/kg feed, 15 mg of Cu/kg feed), the basal diet + 6% rapeseed oil (Diet 1; 18 mg of vitamin E/kg feed, 15 mg of Cu/kg feed), and Diet 1 plus supplements of vitamin E (0, 100, and 200 mg of dl-alpha-tocopheryl acetate/kg feed) and copper (0, 35, and 175 mg of Cu/ kg feed) in a 3 x 3 factorial arrangement of treatments. Eight or nine pigs were given ad libitum access to each diet from 25 to 100 kg of live weight. The inclusion of rapeseed oil tended (P < .10) to improve ADG and feed utilization. Compared with the addition of 35 mg of Cu/kg, the addition of 175 mg/kg improved growth rate and increased feed intake early in the experiment, but, over the total experiment, neither 35 nor 175 mg of Cu/kg affected performance. Compared with the addition of 100 mg of vitamin E/kg or no addition, the addition of 200 mg/kg reduced ADG over the total experiment (P = .05). The antioxidative and oxidative status of the pigs was evaluated in terms of blood and liver concentrations of antioxidants (alpha-tocopherol, ascorbic acid, vitamin A, superoxide dismutase, glutathione peroxidase), prooxidants (Cu), concentrations of lipids (triglycerides and cholesterol), fatty acid composition, thiobarbituric acid-reactive substances (TBARS), and clinical chemical (creatine kinase and glutamate-oxaloacetate-transaminase) and hematological variables that indicate the level of oxidative stress. There were no vitamin E deficiency signs or increased oxidative stress in pigs fed low dietary vitamin E levels, and no prooxidative effect of Cu was found. Increasing dietary levels of vitamin E increased the concentration of alpha-tocopherol in plasma and liver. Supplementation with Cu increased liver concentrations of Cu and alphatocopherol. The progression in liver TBARS was reduced by the addition of vitamin E and Cu. The addition of rapeseed oil changed the fatty acid composition of liver, increased alpha-tocopherol concentration in plasma and Cu concentration in liver, and reduced the rate of lipid oxidation in liver. In conclusion, even though the effects were minor, vitamin E, Cu, and rapeseed oil improved the antioxidative status of the live pigs.     

不同铜源对生长猪生产性能的影响

本试验采用单因素试验设计,选取48头平均体重为39kg的生长猪（杜×大×长）,按照体重接近、公母各半的原则随机分为4个处理组,每个处理3个重复,每个重复4头猪。4个处理组分别为：基础日粮（对照组）,即处理组1：处理组2、3、4分别在基础日粮中添加150mg／kg碱式氯化铜、200mg／kg碱式氯化铜、200mg/kg五水硫酸铜。探讨碱式氯化铜对生长猪生产性能的影响,并确定适宜添加量。试验结果表明：在日粮中添加200mg／kg碱式氯化铜能够显著提高生长猪的平均日增重（P〈0．05）,降低平均日采食量（P〉0．05）,改善料重比（P〉0．05）。因此,碱式氯化铜能有效替代五水硫酸铜作为生长猪的促生长剂。     

Antioxidative and oxidative status in muscles of pigs fed rapeseed oil, vitamin E, and copper

The susceptibility of a given muscle tissue to lipid oxidation may not only depend on the presence of unsaturated fatty acids and the balance between antioxidants and prooxidants, but also on the composition of the skeletal muscle. In the present study, the effects of dietary supplementation of vitamin E (dl-alpha-tocopheryl acetate) and copper in combination with a high level of monounsaturated fatty acids were examined with regard to the antioxidant concentration and the susceptibility to lipid oxidation of two muscles, longissimus (LD) and psoas major (PM), representing different oxidative capacity. In addition, fatty acid profiles of the backfat and the intramuscular lipids, as well as fresh meat quality traits, were studied. Pigs were allotted to a 3x3 factorial experiment with three levels of dl-alpha-tocopheryl acetate (0, 100, and 200 mg/kg of feed) and three levels of copper (0, 35, and 175 mg/kg of feed) added to a diet containing 6% rapeseed oil. A basal diet (without rapeseed oil) was added to the experimental design, giving a total of 10 dietary treatments. Muscle alpha-tocopherol concentrations increased (P<.001) with increasing dl-alpha-tocopheryl acetate in the feed. The antioxidative status was higher in PM than in LD, when considering the concentration of alpha-tocopherol (P<.001) and the activity of antioxidant enzymes (superoxide dismutase, P<.001; glutathione peroxidase, P = .06). Supplemental copper did not give rise to any deposition of copper in muscle tissue or backfat, but the antioxidant status of PM increased. The susceptibility to lipid oxidation was reduced in LD with increasing dietary dl-alpha-tocopheryl acetate and in PM with increasing dietary copper. Supplemental dl-alpha-tocopherol acetate improved the water-holding capacity of LD (P = .005) and PM (P = .003). The fatty acid composition of the backfat and the triglyceride fraction of the intramuscular fat became more unsaturated with the addition of rapeseed oil to the feed. Higher intakes of monounsaturated fatty acids due to the rapeseed oil were also reflected in the phospholipid fraction of the intramuscular fat, but no influence on the proportion of saturated fatty acids was seen. The susceptibility to lipid oxidation of PM was lower for pigs on the rapeseed oil-based diet than for those on the basal diet. The energy metabolic status of the muscles and the accumulation of calcium by the sarcoplasmic reticulum were not influenced by the dietary treatments, but there were differences between muscle types. The addition of rapeseed oil to the diet reduced the muscular content of glycogen (LD, P = .02; PM, P = .06) and elevated the plasma concentration of free fatty acids (P = .05). Overall, dietary fat, dl-alpha-tocopherol acetate, and copper affected the oxidative status of pig muscles, and the results differed depending on muscle type.     

BGB饲料对生长猪和肥育猪生长性能的影响

本试验旨在评估BGB饲料对生长猪和肥育猪生长性能的影响。试验分两期进行,试验1选用初始体重为19.02±0.04kg的阉公猪72头,随机分为3组。以玉米-豆粕型日粮为基础日粮,对照组添加60mg/kg盐霉素,试验组分别添加5%、10%的BGB饲料。试验期28d,计算日采食量(ADFI)、日增重(ADG)和饲料转化率(FCR)。试验2选用初始体重为57.01±0.6kg的阉公猪54头,试验期35d,试验期日粮处理与饲养管理同试验1。试验1结果表明:处理组与对照组各项测定指标无显著差异(P>0.05),但在饲喂后期,5%BGB添加组的ADG比对照组提高7.8%,饲料转化率改善10.8%。试验2结果表明:处理组与对照组各项测定指标无显著差异(P>0.05),在饲喂后期,5%、10%BGB添加组的ADG分别比对照组改善了10.7%和4.0%。从全期结果来看,处理组的效果要优于对照组,5%BGB添加组效果最好。以上两个试验表明:添加BGB可以达到与添加抗生素相似的生产水平,对生长性能有一定的促进作用,最适添加量为5%。     

茶多酚对生长猪机体维生素E状态、抗氧化性能及肉质的影响

日粮添加维生素E对降低动物脂质过氧化具有重要作用,可以改善动物肉品质及动物健康状况。本试验在日粮中添加不同水平的茶多酚,研究其对生长猪维生素E状态、血浆抗氧化性能及肉质的影响。选择240头平均体重为(31.24±0.48)kg的三元猪,随机分为3组,每组4个重复,每个重复20头猪。试验共设计3种日粮,对照组饲喂基础日粮,处理组在基础日粮中分别添加10和100 mg/kg茶多酚,试验共进行5周。结果显示:各处理组对生长猪试验初期和末期血浆总抗氧化力和铁还原力均无显著影响(P> 0.05),但对照组、10和100 mg/kg茶多酚添加组血浆试验末期铁还原力均显著高于试验初期(P <0.05)。日粮添加茶多酚对生长猪肝脏、肌肉、血浆α-生育酚和γ-生育酚含量均无显著影响(P> 0.05),其中肝脏生育酚含量最高,之后依次是肌肉、肺和血浆。日粮添加10或100 mg/kg茶多酚,肺中α-生育酚和γ-生育酚含量较对照组有提高趋势(P=0.09)。各组对生长猪肉质均无显著影响(P> 0.05)。日粮添加100 mg/kg茶多酚较对照组提高了生长猪屠宰后48 h肌肉的滴水损失。综上所述:生长猪日粮添加茶多酚对组织维生素E水平、血浆抗氧化力及肌肉品质无显著改善作用。     

Dietary green tea polyphenols do not affect vitamin E status,antioxidant capacity and meat quality of growing pigs

Supplementation of pigs with vitamin E, the most important lipid-soluble antioxidant, has been shown to improve meat quality and animal health. Previous studies in cultured cells and laboratory animals indicate synergistic effects between polyphenols and vitamin E. The present feeding trial was undertaken to investigate the effects of dietary green tea polyphenols (GTP) on vitamin E status, antioxidative capacity and parameters of meat quality in growing pigs. Eighteen castrated, crossbred, male pigs received a flavonoid-poor diet based on corn starch, caseinate and rapeseed oil with a total vitamin E content of 17 IU/kg diet over a period of 5 weeks. This basal diet was supplemented with green tea extract to provide daily doses of 0 (control), 10 and 100 mg GTP/kg body weight. Dietary supplementation of growing pigs with GTP did not affect serum, liver, lung and muscle vitamin E (α- and γ-tocopherol) concentrations, plasma antioxidant capacity (ferric reducing ability of plasma, trolox equivalent antioxidant capacity) or parameters of meat quality including meat temperature, pH, conductivity, colour and drip loss. In conclusion, supplementation of pig diets with green tea catechins is not associated with improved antioxidant status and meat quality under practice-oriented conditions.     

不同药物添加剂对生长猪生产性能的影响

试验选用长大二元阉公猪 10 2头 ,分别随机分为 5组 ,对照组饲喂由牛至油按2 4mg/kg配置的日粮、其他各组分别在基础日粮中添加 10 %效美素 15 0mg/kg 15 %吉它霉素2 0 0mg/kg为试验 1组 ;添加 0 .2 5 %那西肽 10 0 0mg/kg 15 %吉它霉素 2 0 0mg/kg为试验 2组 ;添加 0 .2 5 %那西肽 10 0 0mg/kg为试验 3组 ;添加 15 %吉它霉素 4 80mg/kg为试验 4组。试验结果 :①使用抗生素的各组生产性能和经济效益均优于牛至油组。②试验 2组有最快的增重速度和较低的饲料成本 ;③试验 3组饲料成本最低 ,且日增重较高 ,这 2组的综合经济效益最好。④试验1组虽然有较高的日增重和饲料转化率 ,但因饲料单价高 ,造成饲料成本比较高。而试验 4组由于日采食量低 ,造成增重速度比其他抗生素组慢 ,出栏时间延长。     

Effect of selenium, vitamin E and riboflavin supplementation of the feed on the humoral and cell-mediated immune responses of growing pigs

The feed of weaned piglets of Hungarian Large White X Duroc and Dutch Landrace X Duroc genotype was supplemented with 0.5 mg selenium, 50, 100 or 150 mg vitamin E, and 2.5 or 5 mg riboflavin per kg. Feed supplementation enhanced the cytotoxic reaction and elevated the antibody titres produced against purified horse gamma globulin antigen. However, as compared to the control the differences were not significant. Feed supplementation exerted a beneficial, though varying, influence on the indices of cell-mediated immunity. The proportion of rosette-forming cells and blastogenic transformation induced by specific (horse globulin) and nonspecific (phytohaemagglutinin, PHA) mitogens underwent the most expressed and most significant increase in pigs fed 5 mg selenium, 100 mg vitamin E and 5 mg riboflavin per kg of feed. On the other hand, feed supplementation failed to enhance the responsiveness to intradermal PHA (type IV allergic reaction).     

Effects of dietary copper concentration and source on performance and copper status of growing and finishing steers

Performance and Cu status were measured in growing and finishing steers supplemented with different copper (Cu) concentrations and sources. Sixty Angus (n = 36) and Angus x Hereford (n = 24) steers were stratified by weight and initial liver Cu concentration within a breed and randomly assigned to treatments. Treatments consisted of 1) control (no supplemental Cu); 2) 20 mg Cu/kg DM from Cu sulfate (CuSO4); 3) 40 mg Cu/kg DM from CuSO4; 4) 20 mg Cu/ kg DM from Cu citrate (C6H4Cu2O7); 5) 20 mg Cu/kg DM from Cu proteinate; and 6) 20 mg Cu/kg DM from tribasic Cu chloride (Cu2(OH)3Cl). A corn silage-soybean meal-based diet that was analyzed to contain 10.2 mg of Cu/kg DM was fed for 56 d. Steers were then switched to a high-concentrate diet that was analyzed to contain 4.9 mg of Cu/kg DM. Equal numbers of steers per treatment were slaughtered after receiving the finishing diets for either 101 or 121 d. Performance was not affected by Cu level or source during the growing phase. Gain, feed intake, and feed efficiency were reduced (P < .05) by Cu supplementation during the finishing phase. Plasma and liver Cu concentrations were higher in steers receiving supplemental Cu at the end of both the growing and finishing phases. Steers supplemented with 40 mg Cu/kg DM from CuSO4 had higher (P < .05) liver Cu concentrations than those supplemented with 20 mg Cu/kg DM from CuSO4. Liver Cu concentrations did not increase over the finishing phase relative to liver Cu concentrations at the end of the growing phase. These results indicate that as little as 20 mg/kg of supplemental Cu can reduce performance in finishing steers.     

不同饲粮蛋白质水平对生长肥育猪生产性能的影响

蛋白质饲料是肉猪生产的重要物质基础,是决定生产成本的重要因素。为了探索饲粮蛋白质水平对生长肥育猪增重速度、饲料利用率及育肥猪肉品品质等的影响作用,确定肉猪合理的饲粮结构以获得更大的经济效益,进行了本试验。     

松针粉对生长猪生长性能及免疫功能的影响

为探究饲料中添加松针粉对生长猪生长性能及免疫功能的影响,选择品种、胎次相近,平均体重为(60.92±0.46)kg的杜×长×大(Duroc×Landrace×large white)三元杂交猪108头,按完全随机设计法分为3组,每组6个重复,每个重复6头猪,试验周期41 d。对照组饲喂基础日粮,试验A组饲喂基础日粮+2%松针粉(等量替代麸皮),试验B组饲喂基础日粮+2%松针粉(直接添加)。结果表明:与对照组相比,试验A组的日增重提高2.88%,采食量提高1.94%,料肉比降低3.91%(P > 0.05);试验组B日增重降低3.43%,采食量降低3.91%,料肉比降低 0.53%(P > 0.05);试验A组与B组的咳嗽率均有所降低,分别降低64.22%和53.43%(P > 0.05);试验A组与B组血清中免疫球蛋白IgG和IgM含量较高,但三个试验组之间差异不显著。因此,在饲料中用2%松针粉等量替代麸皮或直接添加2%松针粉均有提高猪只生长性能、降低咳嗽率、提高免疫功能的趋势(P > 0.05)。     

The effect of polychlorinated biphenyls on the vitamin A, vitamin E, and ascorbic acid status in young pigs

Admixture for 14 to 26 days of concentrations between 1 mg/kg to 250 mg/kg of polychlorinated biphenyls, Delor 103, 105, and 106 (Chemko, Strázeke) to ready-mixed feedstuffs was studied in 5 experiments for its effects on 71 piglets and store pigs. Neither growth nor general health were in any way impaired. Polychlorinated biphenyls produced different effects upon vitamin A, vitamin E, and ascorbic acid levels in different organs. Vitamin A concentrations in blood serum, for example were reduced by 100 mg/kg and 250 mg/kg of Delor 105 as well as by 250 mg/kg of Delor 103. Liver levels were not significantly depressed. No changes were recordable from vitamin E levels in blood serum and liver. Ascorbic acid saturation of the organism was positively affected. Its concentrations and overall levels in the liver in all experimental groups were higher than those in all controls. Significant rise of ascorbic acid concentrations in blood serum, urine, and kidneys occurred merely in response to 100 mg/kg and 250 mg/kg of Delor 105 as well as to 250 mg/kg of Delor 103. No adverse effect of tissue-borne residues of polychlorinated biphenyls on the status of administered vitamins was recordable 1 to 4 months after termination of experimental supply. Feed intake with 1 mg/kg of Delor 105, which was equivalent to increased natural contamination, did not cause any negative phenomena in any of the probands.     

膨化全脂油菜籽粉对生长猪生产性能的影响

选择体重为20kg±2kg的健康杜×长×大生长猪60头,随机分为5处理（每处理3重复,每重复有4头猪）,分别饲喂0％、5％、10％、15％、20％膨化全脂油菜籽粉（EFFRM）日粮,各处理日粮中EFFRM以等能等可消化氨基酸基础取代大豆蛋白。试猪自由采食和饮水,试验期40d。试验用全脂菜籽为“双高”品种,采用干法膨化加工。试验结果显示：试猪采食量和日增重随日粮中EFFRM用量的增加呈先上升（5％用量）后明显下降（10％～20％用量）的规律;5％EFFRM处理的采食量和增重在数值上高于0％处理,差异未达显著水平（P〉0．05）,但显著高于10％处理（P〈0．05）,极显著高于15％、20％处理（P〈0．01）：各处理的单位增重耗料（FCR）无显著差异（P〉0．05）,在数值上仍以5％、0％处理最低。以上结果表明,20-40kg猪日粮中“双高”来源的EFFRM用量以5％为宜,用量10％及以上会显著影响猪的采食量,降低生长速度。     

不同添加水平的维生素E对育肥猪肉质的影响

试验研究了在屠宰前期育肥猪的日粮中添加不同水平的维生素E,对肥育猪肉质的影响。将32头体重80kg左右的二元杂交猪,随机分成对照组和3个试验组,每组8头猪。在饲喂基础日粮的前提下,试验组Ⅰ、Ⅱ、Ⅲ添加维生素E的量分别为100、200、300mg/kg,对照组不添加维生素E。试验结果表明:在日粮中添加100、200、300mg/kg的维生素E,能不同程度地改善试验育肥猪的肉色,提高肌肉的系水力,降低滴水损失,减少肌糖原酵解,抑制肌乳酸生成,降低肌肉蛋白的腐败变质程度,从而延长货架期。同时试验还证实了维生素E的最适添加量为200mg/kg。     

维生素E对羔羊成活率和生产性能的影响

维生素Ｅ是所有具有α -生育酚功能的母育酚和生育三烯醇衍生物的总称。放牧到春天青草茂盛草场上的母羊 ,一般含有高浓度的血清维生素Ｅ。相比之下 ,饲喂贮藏干草或休牧期的母羊 ,通常含有较低的血清维生素Ｅ浓度。母羊血清维生素Ｅ水平产前 7ｄ可降低约 50 % ,直到产后 2 0～ 3 0ｄ才会恢复至正常水平。因此有理由推测休牧期或喂给收割饲料的怀孕绵羊在冬末春初产羔之前 ,为提高羔羊生产力需补充维生素Ｅ。脂肪组织是机体储存维生素Ｅ的主要部位 (Ｐｕｌｓ ,1 994)。然而维生素Ｅ不可能以合理的浓度被储存于机体内 (Ｒａｍ ｍｅｌｌ,1 983…     

Effect of vitamin E and selenium on iron utilization in neonatal pigs.

Supplying adequate iron (Fe) to neonatal pigs to support normal growth and hematological and antioxidant status, while preventing iron toxicity, is a challenge for producers. Three experiments were conducted to determine the effect of frequency and route of Fe administration with or without vitamin E (E) and selenium (Se) on growth, Fe, and antioxidant status of neonatal pigs. In Exp. 1, 12 pigs from dams with reduced E status were fed a semipurified diet without added Fe from d 3 to d 14 of age. At d 6 of age, pigs received the following i.m. injections: 1) FE, 1 mL containing 200 mg of Fe (iron dextran); 2) FEE, treatment FE plus 1 mL containing 300 IU of vitamin E (d-alpha tocopherol); or 3) FESEE, 1.03 mL containing 200 mg of Fe (iron dextran), .15 mg of Se (sodium selenite), and 15 IU of vitamin E (d-alpha tocopherol). Pigs were weighed daily and blood was collected at 3, 7, and 14 d of age. From d 8 to 14, growth was depressed (P < .05) in pigs injected with FESEE. At 14 d of age, pigs injected with FE or FEE had increased (P < .05) hemoglobin (Hb) concentration. Ceruloplasmin activity (CP) was greater (P < .05) at d 7 of age than at d 3 or 14 regardless of treatment. In Exp. 2, 3-d-old pigs (n = 94) received the following: 1) FE, 200 mg Fe (iron dextran) i.m.; (2) FEE, treatment FE plus 300 IU vitamin E i.m.; 3) EFE, 300 IU vitamin E i.m. followed by 200 mg Fe (iron dextran) i.m. 24 h later; or 4) OFE, 100 mg Fe and 10 mg Cu orally. On d 21 of age, one-half of the pigs in each treatment received a second dose of their respective treatment. Blood samples (n = 60) were obtained on d 3 and 21 of age. Pigs injected with FE, FEE, or EFE had greater (P < .05) Hb at d 21 than pigs given OFE. Copper/zinc superoxide dismutase (Cu/ZnSOD) activity was greater (P < .05) at d 21 with OFE than with the other treatments. At 65 d of age, ADG did not differ among treatments. In Exp. 3, pigs (n = 150, in three farrowing groups) were injected with 200 mg of Fe (iron dextran) on d 1 or d 1 and 14. Blood samples were obtained on d 7 and 21 of age. Hemoglobin concentration on d 21 was improved equally by both treatments. Catalase and Cu/ZnSOD activities were increased (P < .05) on d 21 of the experiment compared with d 7 regardless of treatment. Growth was not affected by injection frequency. Results from these experiments indicate that one Fe injection (200 mg) for pigs from sows fed adequate vitamin E will result in adequate growth and hemoglobin concentration with today's improved genetics.