Effects of Paeonol on Expression of Type Ⅰ and Type Ⅲ Collagen of High Glucose Induced Proliferation of Cardiac Fibroblasts

[Objectives]To explore the effects of paeonol on the inhibition of myocardial fibrosis in high glucose induced cardiac fibroblasts(CFs).[Methods]Differential ad...     

Study on Quality Standards of Children Antipyretic Lotion

[Objectives]To establish suitable quality standards of children antipyretic lotion.[Methods]Qualitative identification was performed for Paederia scandens and A...     

猪血凝性脑脊髓炎病毒RT-PCR方法的建立及初步应用

为了建立快速检测猪血凝性脑脊髓炎病毒（HEV）的RT-PCR方法,根据GenBank中HEV的HE基因和S基因设计了1对引物,在优化RT-PCR反应条件的基础上,成功的扩增出323bp的特异性条带。检测与HEV亲缘性较高的牛冠状病毒及猪的伪狂犬病毒均为阴性,最低可以检测到10个TCID50/100μl的病毒,说明该方法的有较好的特异性和敏感性。用此RT-PCR方法对感染HEV的小鼠和猪进行检测,结果能从发病动物的多种组织中检测到病原,其中以脑组织的检出率最高。因此,临床疑似病例检测时以脑组织为最佳检测样本     

Putative Identification and Adduct Formation of Reference Materials for Cotton Secondary Metabolite using UPLC-ESI-MS Analysis

[Objective] The aim of this study is to establish an method for cotton metabolites identification by ultra-performance liquid chromatography-electrospray ionization-mass spectrometry (UPLC-ESI-MS) analysis, and to investigate the adduct types, dominant adducts and appropriate ESI ion modes of cotton secondary metabolites under the determined UPLC and ESI conditions. [Method] UPLC-ESI-MS was employed to analyze 18 cotton metabolite standards, Online XCMS software was used to extract the nontargeted mass spectrum data, and MATLAB software was used to prepare calculation programs for the identification method of cotton metabolite standards. [Result] A high-throughput identification method of cotton secondary metabolite, named POSid and NEGid separately for the positive and negative ESI modes, was established based on the calculated accurate molecular weight. In the determined UPLC condition and positive and negative ESI mode, 14 cotton metabolite standards were correctly identified. There found 6 adducts including [M+H]⁺, [M+Na]⁺, [M+NH₄]⁺, [2M+NH₄]⁺, [2M+Na]⁺ and [2M+H]⁺ in positive ion mode, and 8 adducts including [M-H]^-, [2M-H]^-, [M+Cl]^-, [M+FA-H]^-, [3M-H]^-, [M+Na-2H]-, [M-H₂O-H]^- and [M+TFA-H]^- in negative ion mode, while 1 to 6 adducts were observed in the mass spectrum of a single standard, and each metabolite standard had a dominant adduct of a preference for ESI mode. Melibose was suitable for ESI positive ion mode detection, gossypol was suitable for both ion modes detection; and 12 compounds were suitable for negative ion mode detection due to mass spectrum signals of their dominant adduct stronger in negative ion mode than positive ion mode. [Conclusion] Based on the accurate molecular weight, the established identifying method can identify the 18 cotton metabolite standards with their nontarget mass spectrometry data. Under the determined UPLC and ESI condition, the dominant adducts of cotton secondary metabolites have the preference of ESI mode. These results provide technical and theoretical data support for further study of cotton metabolomics.     

Study Crystallinity of the Developing Cotton Fibers by Micro-Fourier Transform Infrared Spectroscopy (FTIR) and X-Ray Diffraction (XRD)

[Objective] The change of crystalline index (CI) of two different cultivated cotton fibers at the development stage was studied by micro-Fourier transform infrared spectroscopy (micro-FTIR) and X-ray diffraction (XRD). The feasibility was verified by measuring CI in developing cotton fibers with micro-FTIR method. And it was utilized to estimate the CI of mature cotton fibers. [Method] Upland cotton 0-153 and sea island cotton S-6 were selected as examined materials. The cotton fibers of 2 varieties were obtained at 5-30 d post anthesis respectively, with a sampling interval of 5 d. After obtaining the fiber, it was washed several times with distilled water and then placed in an oven at 40℃ for 48 hours. After drying, the FTIR and XRD spectra of each sample were obtained. The CI is calculated according to 4 different FTIR-CI calculation methods, the crystallinity changes of different cotton varieties in the developing period were compared, and the correlation between the FTIR-CI and XRD-CI was studied. [Result] Only FTIR-CI calculated by the Carrillo-Colom index(FTIR-CCI) had a good correlation with XRD-CI method, with higher R2 than 0.9 of both varieties. The fitting model (IR-CI) between the FTIR-CCI and XRD-CI was used to calculate the CI of the twenty-three randomly selected mature fibers. The results showed that the accuracy of IR-CI was good, and the XRD-CI results were within the error range of the calculated results according to the IR-CI model, while the precision of IR-CI could not reach anticipation. [Conclusion] Micro-FTIR can be used to study the change of cellulose in cotton fiber during the developing period. The model of IR-CI established by the correlation between the FTIR-CCI and XRD-CI, can be used to evaluate the crystallinity of developing period in cotton fiber. However, for the study of the crystallinity of mature fibers, it is necessary to use a large number of samples in the later experiment to establish an optimized model.     

高粱与苏丹草杂种优势利用的研究

高粱与苏丹草杂种优势明显,F1单株生物产量性状高于双亲平均值,甚至超过或接近高亲。影响高粱-苏丹草杂交种单株茎叶鲜重的主要因素是主茎粗、叶长;决定单位面积鲜草产量的主要是密度、叶长和单株茎叶鲜重。多年随机区组试验结果表明,杂交种单位面积鲜草产量显著超过苏丹草,并以30万株/hm2为最适种植密度。杂交种营养品     

Stability of Sanguinarine and Chelerythrine in Macleaya cordata (Willd.) R. Br.

[Objectives]To explore the stability of sanguinarine and chelerythrine in Macleaya cordata(Willd.)R.Br.[Methods]The solubility and stability of sanguinarine and...     

甘蓝型油菜透明种皮12（TT12）基因家族反义抑制植物表达载体的构建

【研究目的】构建一个同时反义共抑制甘蓝型油菜(Brassica napus)透明种皮12基因家族(BnTT12)转录的植物表达载体。【方法】通过PCR扩增得到BnTT12家族一段503bp的特异保守片段TT12A,通过亚克隆整合到中间载体pCambia2301G,构建TT12反义植物表达载体pCambi-a2301G-TT12A。采用液氮冻融法将其转化到根癌农杆菌。【结果】经过PCR鉴定、酶切鉴定和DNA测序证实载体构建成功,并转化到根癌农杆菌LBA4404菌株中形成TT12反义表达载体的工程菌株。【结论】pCambia2301G-TT12A的成功构建为利用此反义载体通过遗传转化获得转基因新型黄籽油菜和进一步探明TT12基因在甘蓝型油菜种皮色素合成途径中的分子生物学机理奠定了基础。     

低温胁迫对小麦、玉米、萝卜幼苗超氧化物歧化酶活性的影响

摘 要：[目的]研究低温胁迫对小麦、玉米、萝卜幼苗SOD活性的影响及三种植物受影响程度的差异。[方法]以萝卜,玉米,小麦幼苗为实验材料,采用模拟冷害的实验方法,通过氮蓝四唑自氧化法测定SOD活性,从胁迫温度变化和胁迫时间变化两个角度研究低温胁迫对小麦、玉米、萝卜幼苗SOD活性的影响。[结果]随着胁迫温度的降低,小麦、玉米、萝卜幼苗的SOD活性都呈现先上升后下降的趋势,且0℃胁迫组酶活性低于常温组;随着胁迫时间的变化,小麦、玉米、萝卜幼苗的SOD活性走势不同,但0℃胁迫时,酶活性变化不大,且低于常温组酶活。[结论]适当的低温处理可以增强SOD活性,超过植物耐受范围后,酶活性遭到抑制而降低。     

转bar基因小麦的抗性遗传及农艺性状分析

【研究目的】以皖麦48和扬麦87158两个小麦品种的转bar基因株系为材料,探讨bar基因在转基因小麦自交后代的遗传表现及对农艺性状的影响。【方法】利用涂叶法和PCR法研究bar基因在转基因植株自交后代T1、T2的遗传表现,并对产量及品质等主要农艺性状进行比较分析。【结果】证实抗除草剂bar基因已经整合到小麦基因组中,并能稳定表达;遗传分析显示bar基因能稳定的遗传给后代,符合孟德尔遗传规律;在产量及品质等主要农艺性状方面,转bar基因小麦与对照相比无显著差异。     

应用叶片保护率评价阿维菌素对小菜蛾的毒力

研究目的】尝试采用叶片保护率评价阿维菌素对小菜蛾（Plutella xylostella (Linnaeus)）2龄、3龄幼虫的毒力,以期为该药毒力的准确评价提供资料。【研究方法】浸叶法测定阿维菌素对小菜蛾2龄、3龄幼虫室内毒力的同时,观察记录24h、48h、72h校正死亡率、叶片保护率【结果】对小菜蛾2龄幼虫,24h、48h、72h的LC50分别为>10.00mg/L、2.87mg/L、0.56mg/L;相同时间内PC50分别为0.25 mg/L、0.82mg/L、1.43mg/L。对3龄幼虫,24h、48h、72h的LC50分别为>10.00mg/L、6.20mg/L、3.00mg/L;相同时间内PC50分别为<0.16mg/L、0.04mg/L、4.25mg/L。【结论】根据叶片保护率,可以发现阿维菌素对小菜蛾影响快,但是当阿维菌素毒力达到稳定时,必须有较高的校正死亡率,否则达不到理想的叶面保护效果。     

不同光质对菊花组培苗生长的影响

本文研究新型光源LED辐射的不同光质对菊花组培苗生长的影响,以期为植物组培专用LED光源的研发提供数据支持和理论依据。以菊花组培苗为试材,采用LEDs光源发射的单色光谱红光[(658±20) nm]、蓝光[(460±20) nm]、远红光[(730±20) nm]和绿光[(530±20) nm],进行不同光质配比组合,荧光灯作为对照,对组培苗形态、生根,色素含量,碳氮代谢及抗氧化酶系活性进行差异比较。菊花组培苗在红光下徒长,能效最大。蓝光下矮壮,根系活力最大,复合LEDs光质下,组培苗形态正常。RBG处理的菊花组培苗叶片色素含量最高。红光有利于叶绿素b的合成,蓝光有利于叶绿素a的合成。单频红光处理的菊花叶片淀粉含量最高,RBG处理的叶片可溶性糖、碳水化合物蔗糖、游离氨基酸含量最高。蓝光有利于蛋白质的合成,LEDs光质处理的叶片C/N比高于荧光灯。随着LEDs技术的改进与发展, LEDs光照系统将会替代荧光灯成为植物组织培养的理想光源。     

Study on Colorimetric Method and Content Determination of Total Phenol in Homemade Plum Wine

[Objectives] To study on colorimetric method and content determination of total phenol in homemade plum wine. [Methods]The optimal determination condition of to...     

不同遮荫方式及遮荫率对滇重楼生长的影响研究

摘要：【研究目的】：探讨不同遮荫方式及遮荫度对云南重楼根茎增重倍数、存活率及产量的影响,为确定合理的栽培措施提供依据。【方法】：比较了遮阳网、向日葵、玉米、三分三4种不同遮荫方式条件下云南重楼生长情况。【结果】：优选出最适宜的遮荫方式和遮荫度。【结论】：10g左右的种苗移栽种植3年期间,最佳的重楼遮荫方式采用遮阳网,遮荫率在60%左右。     

牛病毒性腹泻/黏膜病病毒河北分离株的生物学特性

【目的】了解牛病毒性腹泻病毒河北分离株HB株的特性，阐明牛病毒性腹泻/黏膜病致病机理，更好的控制BVDV感染、流行提供理论依据特进行此项试验；【方法】用理化学及生物学方法对BVDV河北分离毒株HB的特性进行检测，与BVDV准毒株Oregon C24V株进行比较，分析了其理化性及生物学特性。电镜负染检查可见直径为40~60nm有突起的圆形或近圆形的病毒颗粒,病毒在蔗糖中的浮密度为1.13~1.14g/cm3。与牛病毒性腹泻－粘膜病病毒颗粒相似。毒力测定TCID50为10-4.5。理化学研究表明，该病毒对氯仿、乙醚、胰酶敏感；不耐酸、对碱具有较强的耐受性。牛病毒性腹泻/黏膜病病毒对56℃ 30min较敏感，56℃ 70min可使其完全灭活。血凝试验表明，该病毒对鸡、兔、猪和绵羊的红细胞均无血凝性；5-碘脱氧尿核苷(5，-IUDR)不能抑制病毒的增殖，核酸分型试验表明该病毒株基因组为RNA；病毒纯化后经SDS—PAGE电泳出现了5条主要结构蛋白带，与BVDV国际标准毒株Oregon C24V株结果一致；【结论】河北分离株HB株为牛病毒性腹泻病毒。