电子束辐照对小麦储藏抑菌及加工品质的影响

该文研究了不同剂量电子束辐照对储藏小麦微生物菌落的抑制作用,以及辐照后对小麦主要储藏指标与加工品质的影响。试验结果表明,电子束辐照对储藏小麦菌落总数有明显的抑制作用（P＜0.01）,且辐照剂量越高菌落总数越低;不同剂量辐照后,对所有霉菌属的抑制效果均达极显著水平（P＜0.01）。经0～5.1 kGy剂量辐照后,小麦感官及基本营养成分没有太大变化;面团流变学特性表明,除稳定时间和断裂时间随着剂量的增加略增外,评价值与弱化度呈负相关,其他吸水率、形成时间的变化并不十分明显;但高剂量照射使其面筋指数、α-淀粉酶活性迅速降低,经5.1 kGy剂量辐照的比未经辐照的小麦降落数值下降了56.12%。馒头制品品尝评分也随着辐照剂量的增大而降低。     

磷肥用量对土壤中溶磷青霉菌 P8接种效果的影响

溶磷微生物对土壤中难溶无机磷的转化具有重要作用,在生产中,磷肥的施用是否会对溶磷微生物的接种效果产生影响,如何实现溶磷菌肥和磷肥的合理施用,是值得深入探讨的问题。以玉米、花生、小麦为试验作物,在使用磷肥的条件下,通过溶磷菌株青霉菌P8对作物生物量、吸磷量和土壤有效磷含量影响的研究,探讨磷肥对青霉菌P8接种效果的影响。结果表明,盆栽试验第1茬,与单施磷肥相比,施用磷肥同时接种青霉菌未明显增加玉米、花生的生物量,但是接种青霉菌P8促进了对磷素的吸收;盆栽试验第2茬,在土壤有效磷降低的情况下,青霉菌P8显著增加了玉米、花生的生物量和吸磷量,而且青霉菌P8具有较强的溶磷后效,接种青霉菌P8的土壤有效磷含量高于不接种处理的土壤;在小麦盆栽试验中,接种青霉菌P8增产作用显著,随着P8菌剂用量的增加小麦生物量逐渐提高,青霉菌与磷肥配合施用可以有效地提高小麦吸磷能力;使用磷肥能够提高青霉菌P8在土壤中的存活数量,灭菌土壤青霉菌P8的生存效果高于非灭菌土壤。     

储藏时间影响小麦蛋白质理化特性的灌溉效应

灌溉对小麦蛋白质理化特性的影响规律报道不完全一致,这可能与检测蛋白质理化特性时籽粒储藏时间长短有关。为明确灌溉效应受籽粒储藏时间的影响,进一步明确灌溉对小麦蛋白质理化特性的影响,本试验将3种灌溉处理水平的衡4399小麦籽粒在室温条件下储藏120 d,每30 d取样一次,用布勒磨磨制小麦粉(出粉率为66%~71%),检测面粉的蛋白质数量属性、质量属性和面团流变学特性。结果表明,灌溉和储藏时间以及二者的交互作用均显著影响小麦蛋白质理化特性。配对T检验显示,面粉粗蛋白含量和湿面筋含量随灌溉量及频次的增加分别相对降低6%和14%;沉降指数、面团稳定时间和面团最大拉伸阻力随灌溉量及频次的增加分别相对增加15%、33%和95%。进一步多重比较表明,储藏时间影响了麦谷蛋白大聚体含量及其储能模量值、面团吸水率和拉伸长度的灌溉效应。储藏2~3个月后小麦蛋白质理化特性指标逐渐趋于稳定。籽粒储藏时间影响小麦蛋白质理化特性的灌溉效应,籽粒收获后至少储藏90 d再检测才能客观反映灌溉效应。本研究为进一步揭示灌溉对小麦品质的影响规律提供了参考。     

臭氧结合氮气气调储藏对稻谷品质的影响

为探讨臭氧(O3)预处理结合氮气(N2)气调对稻谷储藏品质的影响,以4种不同方式(O3/N2、O3/Air、对照/N2、对照/Air)对稻谷进行处理,并在14℃和相对湿度75％的储藏条件下,对储藏期间稻谷微生物指标、理化特性和感官评分等进行研究.结果表明,臭氧预处理显著降低了稻谷中细菌、霉菌和酵母菌菌落数(P＜0.05...     

生物质炭对小麦根区土壤养分和微生物特征的影响

利用控制性的大田试验栽培技术,连续5年研究了不同浓度生物质炭(对照CK,0 t/hm~2,低生物质炭LB,10 t/hm~2,中生物质炭MB,20 t/hm~2,高生物质炭HB,40 t/hm~2)对小麦生长过程中根区土壤特性和微生物特性的影响。结果表明:(1)小麦根区土壤pH值、容重随生物质炭浓度的增加呈逐渐降低趋势,不同浓度生物质炭处理下的小麦根区土壤pH值、容重均显著低于对照(p0.05);(2)小麦根区土壤有机碳、全氮、全磷和全钾含量均随生物质炭浓度的增加呈先增加后降低趋势,MB浓度处理下,小麦根区土壤有机碳、全氮和全钾达到最大,不同浓度生物质炭处理下土壤有机碳、全氮和全钾均显著高于对照(p0.05),全磷差异并不显著(p0.05);(3)小麦根区土壤微生物数量以细菌最多(占到微生物总数的90%以上),其次是放线菌,真菌最少,小麦根区土壤细菌数量和放线菌数量,随生物质炭浓度的增加呈先增加后降低趋势,MB浓度处理下,小麦根区土壤细菌数量和放线菌数量和最大,并且显著高于对照(p0.05),而生物质炭却显著降低了小麦根区土壤真菌数量,总的来说,生物质炭显著增加了土壤微生物总数;(4)小麦根区土壤微生量碳、微生物量氮、微生物呼吸、微生物代谢熵和微生物活度均随生物质炭浓度的增加呈先增加后降低趋势,MB浓度处理下,小麦根区土壤微生量碳、微生物量氮、微生物呼吸、微生物代谢熵和微生物活度达到最大(p0.05)。生物质炭的施用促进了小麦根区土壤养分、微生物数量和微生物特性和改良土壤肥力作用,以中水平生物质炭(MB)处理下效果最好,而高水平生物质炭(HB)可能会有一定的抑制作用,这还与土壤类型、土壤肥力、植物种类和生态环境等密切相关。     

油茶籽储藏品质变化规律及条件优化

为探讨不同储藏条件下油茶籽品质的变化规律及其原因,找出适宜的储藏条件,该文分别以物料含水率、空气相对湿度和储藏温度为单因素,研究油茶籽储藏过程中的含油率、酸值、过氧化值等品质的变化规律及相应的脂肪酶、脂氧合酶活性,并对微生物的生长情况进行统计分析。运用响应面法,综合考察上述因素对油茶籽品质的影响。结果表明:油茶籽的储藏过程存在3至4个月的后熟期,酸值、过氧化值均随含水率、温度和储藏时间的增加而升高。脂肪酶及微生物的繁殖与酸值的关系比较显著。优化的油茶籽储藏工艺参数为:空气相对湿度57.6%～67.4%,储藏温度17.2～20.4℃,物料含水率8.9%～10.4%和储藏时间62.2～110.3d。该研究可为油茶籽储藏及后续加工提高出油率和保障品质提供技术支持。     

长期施用氮磷肥对塿土钾素的影响

以长期定位施肥与小麦—玉米轮作田间试验为平台,研究了施肥对塿土土壤钾素的影响以及冬小麦生育期内土土壤速效钾、缓效钾及微生物量钾的变化。结果表明:氮磷肥不同施用量对土壤全钾基本没影响,而对速效钾、缓效钾及微生物量钾的影响较大。土壤速效钾和缓效钾均随施肥量的增加而减少,而微生物量钾随施肥量的增加而增加,且0—20cm土层土壤速效钾、缓效钾及微生物量钾含量均高于20—40cm土层。小麦生育期内,土壤速效钾和微生物量钾含量的变化一致,均是先上升再下降,再上升,又下降的变化。土壤中微生物量钾与有机碳、全氮均呈显著正相关关系,而与土壤速效钾和缓效钾无显著相关关系。氮磷肥在一定施用量范围内,促进了土壤无机态钾素的消耗,而提高了土壤微生物量钾的含量。土壤钾素的这种变化是作物吸收、施肥等因素共同作用的结果。     

水、氮磷肥及其互作对小麦淀粉糊化特性的影响

为了解豫西半干旱区不同水、肥条件对小麦淀粉品质的调控效应,于2009—2010年度和2010—2011年度在河南省洛阳市农业科学研究院以"洛旱2号"小麦为材料,采用防雨棚池栽种植方式,研究了不同灌水量、施氮量和施磷量及其互作对小麦淀粉糊化特性的影响。结果表明:施氮量和施磷量对小麦淀粉糊化特性均有显著影响(P≤0.05或P≤0.01);随施氮量的增加,淀粉各黏度参数呈增大趋势,施氮量为105 kg.hm 2时达最大值,之后增加施氮量各黏度参数又有所下降;黏度参数在施磷量为168 kg.hm 2时达最大值。在豫西半干旱生态条件下,灌水量对淀粉黏度参数影响不显著,但存在灌水量×施氮量的显著互作效应(P≤0.05或P≤0.01)。从不同水肥处理组合看,淀粉糊化特性以处理组合N105P168W217.5[施氮量105 kg(N).hm 2、施磷量168kg(P2O5).hm 2和全生育期灌水量217.5 mm]最优。相关分析表明,淀粉黏度参数与籽粒产量在2009—2010年度呈显著正相关,而在2010—2011年度相关不显著,说明产量与淀粉品质间存在着协同提高的可能性。     

施氮量对不同小麦品种高分子量麦谷蛋白亚基表达量及品质影响的研究

采用盆栽试验和SDS-PAGE技术,研究了氮肥对强筋和中筋小麦亚基表达量、品质性状及相关关系的影响。结果表明,施用氮肥提高了优质小麦高分子量谷蛋白亚基表达量,对强筋小麦影响较小,而对中筋小麦影响较大。施氮后,强筋小麦的亚基表达量与醇溶蛋白和谷蛋白的含量极显著相关,而中筋小麦的亚基表达量仅与谷蛋白的含量显著相关,说明氮肥对不同亚基组成小麦高分子量谷蛋白表达量及蛋白组分调控有一定差异。施氮后,高分子量谷蛋白亚基表达量与各项加工品质指标呈正相关;强筋小麦的亚基表达量与湿面筋含量、稳定时间、沉降值和评价值相关性达到显著或极显著水平;而中筋小麦的亚基表达量仅与沉降值相关性达到显著水平。     

长期施用氮磷肥对(土娄)土钾素的影响

以长期定位施肥与小麦一玉米轮作田间试验为平台,研究了施肥对(土娄)土土壤钾素的影响以及冬小麦生育期内(土娄)土土壤速效钾、缓效钾及微生物量钾的变化.结果表明:氮磷肥不同施用量对土壤全钾基本没影响,而对速效钾、缓效钾及微生物量钾的影响较大.土壤速效钾和缓效钾均随施肥量的增加而减少,而微生物量钾随施肥量的增加而增加,且0-20 cm土层土壤速效钾、缓效钾及微生物量钾含量均高于20-40 cm土层.小麦生育期内,土壤速效钾和微生物量钾含量的变化一致,均是先上升再下降,再上升,又下降的变化.土壤中微生物量钾与有机碳、全氮均呈显著正相关关系,而与土壤速效钾和缓效钾无显著相关关系.氮磷肥在一定施用量范围内,促进了土壤无机态钾素的消耗,而提高了土壤微生物量钾的含量.土壤钾素的这种变化是作物吸收、施肥等因素共同作用的结果.     

低氮和干旱胁迫对富士和秦冠生长及氮素利用的影响

【目的】以富士（Fuji）、 秦冠（Qinguan）嫁接在平邑甜茶（Malus hupehensis Rehd.）上的当年生盆栽苗为试验材料,采用砂培方法,研究了缺氮胁迫和干旱对富士和秦冠生长情况、 光合参数、 植株各部位氮磷钾含量及氮素利用效率的影响,分析比较了低氮干旱条件下富士和秦冠生长及氮素利用的差异,以期为果树生产高效肥水利用提供理论指导。【方法】试验共设四个处理: 正常氮正常水（ZZ）、 低氮正常水（DZ）、 正常氮干旱（ZG）、 低氮干旱（DG）。氮素和水分均设置两个水平,分别为正常氮（6 mmol/L NO-3-N）、 低氮（0.3 mmol/LNO-3-N）、 正常供水（保持盆中砂子相对含水量为饱和含水量的80%~85%）、 干旱处理（保持盆中砂子相对含水量为饱和含水量的60%~65%）。【结果】富士和秦冠的生物量（茎和叶）、 株高茎粗等生长指标以及光合速率、 气孔导度、 蒸腾速率均为正常氮正常水（ZZ）＞低氮正常水（DZ）＞正常氮干旱（ZG）＞低氮干旱（DG）,并且相对应处理下秦冠的以上指标均高于富士;正常供水下,缺氮处理使富士、 秦冠的根冠比比正常氮处理均有所增加,富士提高了2.05%,秦冠提高了22.40%。富士和秦冠的氮、 磷、 钾含量均表现出正常氮正常水（ZZ）＞低氮正常水（DZ）＞正常氮干旱（ZG）＞低氮干旱（DG）; 氮、 钾元素含量在植株各部位的分布顺序依次是叶＞根＞茎,磷元素则是根＞叶＞茎;光合氮素利用效率（PNUE）和氮素利用效率表现为秦冠处理之间差异极显著,富士处理之间差异不显著;秦冠的PNUE和NUE明显高于富士,在低氮正常水（DZ）处理下,秦冠氮肥利用率比富士高42.07%,在低氮干旱（DG）处理下高64.14%;低氮胁迫下富士和秦冠的NUE显著提高,并且秦冠提高的幅度高于富士。【结论】施用氮肥能够显著提高富士与秦冠的干物质量,同等水肥条件下,秦冠生长优于富士;水分亏缺会减少叶片对氮的吸收,干旱条件下适度增施氮肥,可提高果树的抗旱能力;低氮干旱胁迫下秦冠的生长指标、 光合指标及氮素利用效率指标均优于富士,表现出较强的抗低氮干旱胁迫的能力。     

Pasture Vegetation Elemental Analysis by Laser-Induced Breakdown Spectroscopy

Laser-induced breakdown spectroscopy (LIBS) is a new technique for the analysis of plant material. This study investigates the application of LIBS to pasture-based plant samples. The LIBS measurements were obtained from pelletized pasture samples (100 samples) that had been also analyzed by inductively coupled plasma–optical emission spectroscopy (ICP-OES) following microwave digestion for calibration and comparison purposes. Comparisons for elements sodium (Na), potassium (K), magnesium (Mg), calcium (Ca), manganese (Mn), iron (Fe), copper (Cu), zinc (Zn), boron (B), phosphorus (P), and sulfur (S) showed that LIBS could be used for almost all the standard profile total elements with concentrations down to low mg/kg levels (observed error of Na: 0.024 percent, K: 0.18 percent, Mg: 0.016 percent, Ca: 0.073 percent, P: 0.017 percent, Mn: 31 mg/kg, Fe: 150 mg/kg, Zn: 6.6 mg/kg, and B: 1.1 mg/kg). Elemental analysis at less than mg/kg levels was not possible using LIBS. The elements S and Cu were particularly difficult to analyze with reliability using LIBS at the concentration levels found in the plant samples. Replacing microwave digestion and subsequent ICP analysis with a direct analysis of dried plant samples using LIBS has the potential to improve the productivity and reduce the cost of testing.     

Faculty positions:Center for Agricultural Resources Research,Chinese Academy of Sciences

正The Center for Agricultural Resources Research(CARR),the Institute of Genetics and Developmental Biology(IGDB),Chinese Academy of Sciences,invites applicants for several research group leader positions.CARR is one of the research organizations in Chinese Academy of Sciences(CAS).We seek nominations and applications from individuals who have expertise and a record of accomplishment in research areas related to ecology,agro-hydrology,     

Macroinvertebrates in North American tallgrass prairie soils: effects of fire, mowing, and fertilization on density and biomass

The responses of tallgrass prairie plant communities and ecosystem processes to fire and grazing are well characterized. However, responses of invertebrate consumer groups, and particularly soil-dwelling organisms, to these disturbances are not well known. At Konza Prairie Biological Station, we sampled soil macroinvertebrates in 1994 and 1999 as part of a long-term experiment designed to examine the effects and interactions of annual fire, mowing, and fertilization (N and P) on prairie soil communities and processes. For nearly all taxa, in both years, responses were characterized by significant treatment interactions, but some general patterns were evident. Introduced European earthworms (Aporrectodea spp. and Octolasion spp.) were most abundant in plots where fire was excluded, and the proportion of the total earthworm community consisting of introduced earthworms was greater in unburned, unmowed, and fertilized plots. Nymphs of two Cicada genera were collected (Cicadetta spp. and Tibicen spp.). Cicadetta nymphs were more abundant in burned plots, but mowing reduced their abundance. Tibicen nymphs were collected almost exclusively from unburned plots. Treatment effects on herbivorous beetle larvae (Scarabaeidae, Elateridae, and Curculionidae) were variable, but nutrient additions (N or P) usually resulted in greater densities, whereas mowing usually resulted in lower densities. Our results suggest that departures from historical disturbance regimes (i.e. frequent fire and grazing) may render soils more susceptible to increased numbers of European earthworms, and that interactions between fire, aboveground biomass removal, and vegetation responses affect the structure and composition of invertebrate communities in tallgrass prairie soils.     

Variation in quality parameters between and within 14 Nordic tree fruit and berry species

Abstract

A 3-year study was carried out to investigate quality parameters in 14 tree fruit and berry species grown in southern Norway. The species were blueberry, apple, aronia, sour cherry, sweet cherry, red raspberry, strawberry, blackcurrant, gooseberry, red currant and elderberry, harvested along with wild bilberry, cloudberry and lingonberry. Significant differences between species were identified for all quality parameters. The coefficient of variation between species was lowest for pH (12.5%), dry matter (18.9%) and soluble solids (25.3%), followed by titratable acids (59.3%), total phenolics (83.8%), antioxidant capacity FRAP (85.7%) and antiradical power by the DPPH-assay (97.8%), total monomeric anthocyanins (132%) and ascorbic acid (137%). Average coefficient of variation within species were lower and ranged from 4 (pH) to 62% (ascorbic acid). Only the FRAP values were significantly affected by harvesting year with lower levels in 2004 than in 2005 and 2006. There were significant interactions between species and harvesting year for dry matter, soluble solids, pH, ascorbic acid and FRAP. The results indicate generic ranges in composition within species independent upon growing location and climate, and the composition of the tree fruits and berries is not likely to deviate from these ranges. It is concluded that desirable composition of tree fruits and berries and their products should primarily be achieved by selection among species rather than searching fors broadened variation within individual species.     

Potassium Fixation and Release Characteristics in Rhizosphere and Nonrhizosphere Soils for a Rapeseed–Rice Cropping Sequence

Potassium (K) fixation and release in soil are important factors in the long-term sustainability of a cropping system. Changes in K concentration and characteristics of K fixation and release in rhizosphere and nonrhizosphere soils in the rapeseed (Brassica napus L.)–rice (Oryza sativa L.) rotation were investigated using a rhizobox system. The concentrations of different forms of K in both rhizosphere and nonrhizosphere soils decreased with plants compared to without plants, regardless of K fertilizer application. Potassium uptake by crops mainly came from the rhizosphere soil. In the treatment without K fertilizer (–K), the main form of K supplied by the soil to the crops was 1.0 mol L^?1 nitric acid (HNO₃) nonextractable K, followed by nonexchangeable K, and then exchangeable K. In the treatment with K fertilizer (+K), the main K forms supplied by the soil to the crops were exchangeable K and nonexchangeable K. The amount and rate of K fixation after one cycle of the rapeseed–rice rotation was greater in rhizosphere soil than in nonrhizosphere soil. The amount and rate of K fixation of soil in the +K treatment were significantly less than in the –K treatment. The cumulative amounts of K released with 1.0 mol L^?1 ammonium acetate (NH₄OAc) and 1.0 mol L^?1 HNO₃ extraction increased with the increasing numbers of extractions, but the K-releasing power of soil by successive extraction decreased gradually and finally became almost constant. The release of K was less in rhizosphere soil than in nonrhizosphere soil. The release of K in the +K treatment was similar to that in the –K treatment in rhizosphere soil, but the K release in nonrhizosphere soil was greater with the +K than the –K treatment. Overall, the information obtained in this study will be helpful in formulating more precise K fertilizer recommendations for certain soils.     

Electron donor and pH relationships for biologically enhanced dissolution of chlorinated solvent DNAPL in groundwater

Biologically enhanced dissolution offers a method to speed removal of chlorinated solvent dense non-aqueous-phase liquid (DNAPL) sources such as tetrachloroethene (PCE) and trichoroethene (TCE) from aquifers. Bioremediation is accomplished by adding an electron donor to the source zone where fermentation to intermediates leading to acetic acid and hydrogen results. The hydrogen and possibly acetic acid are used by dehalogenating bacteria to convert PCE and TCE to ethene and hydrochloric acid. Reductive dehalogenation is thus an acid forming process, and sufficient alkalinity must be present to maintain a near neutral pH. The bicarbonate alkalinity required to maintain pH above 6.5 is a function of the electron donor: 800 mg/L of bicarbonate alkalinity is sufficient to achieve about 1.2 mM TCE dechlorination with glucose, 1.7 mM with lactate, and a much higher 3.3 mM with formate. Laboratory studies indicate that in mixed culture, formate can be used as an electron donor for complete conversion to ethene, contrary to pure cultures studies indicating it cannot. Various strategies can be used to add electron donor to an aquifer for DNAPL dehalogenation while minimizing pH problems and excessive electron donor usage, including use of injection-extraction wells, dual recirculation wells, and nested injection-extraction wells.     

Biodiversity and ecogeography of wild Lactuca spp. in some European countries

Staff members of the Department of Botany of Palacký University in Olomouc and Gene Bank Department – Workplace Olomouc, Research Institute of Crop Production in Prague, Czech Republic, conducted an expedition in seven European countries (Austria, Czech Republic, France, Germany, Italy, the Netherlands, Switzerland) in August/September 1999 to collect wild Lactuca spp. germplasm and study its geographic distribution, ecology and biodiversity. During the mission, more than 600 locations were visited resulting in the collection of 602 seed samples (accessions) of wild Lactuca species and 13 seed samples of related genera (Chondrilla and Mycelis). Lactuca serriola f. serriola, L. serriola f. integrifolia, L. saligna and L. viminea subsp. chondrilliflora were prevalent in southern Europe (Italy, France), however, only L. serriola was common in central and western Europe (Austria, Czech Republic, Germany, Netherlands, Switzerland). The greatest diversity of Lactuca species was found in France, where also the most seed samples (165) were collected. The most characteristic habitats with a high density of Lactuca spp. populations were observed along roads and highways, grassy ditches, ruderal communities, and dust-heaps. Natural infections by powdery mildew (Erysiphe cichoracearum) and downy mildew (Bremia lactucae) on some wild Lactuca spp. were observed. Recent observations concerning the geographic distribution, population structure, habitats, and natural occurrence of diseases of Lactuca spp. are discussed. This assemblage of genetic resources of Lactuca spp. can serve as the basis of future studies of species diversification, spatial population structure, plant microevolution, domestication processes, and genetic variability of host-parasite interactions.     

Increase in auxiliary photoprotective photosynthetic pigments in wheat seedlings induced by Azospirillum brasilense

Inoculation of wheat seedlings with the plant growth-promoting bacterium Azospirillum brasilense Cd was immobilized in alginate microbeads and, without applying any stress, significantly increased the quantity of several photosynthetic pigments, such as chlorophyll a, chlorophyll b, and the auxiliary photoprotective pigments violaxanthin, zeaxanthin, antheroxanthin, lutein, neoxanthin, and β-carotene. This resulted in greener plants with no apparent visible stress. After monitoring the quantity of photosynthetic pigments for 4 weeks, we observed that inoculated plants had higher quantities of pigments in shoot and stem. The greatest difference in the quantity of all pigments between inoculated and noninoculated plants occurred in the first week of growth. Regardless of treatment, the quantity of pigments in stems was three to four times less than the quantity of these pigments in shoots. Application of Azospirillum, either as liquid inoculant or as alginate microbeads, did not alter the positive effect of the bacteria on pigment production or the positive response of the plants towards A. brasilense Cd inoculation.     

In-situ enrichment and analysis of atrazine-degrading microbial communities using atrazine-containing porous beads

We examined the community composition of microbes that colonized atrazine-containing beads buried in agricultural soils that differed in atrazine treatment history. Bacterial abundance was 5-40-fold greater in atrazine-fortified beads. In beads containing 20 mg atrazine kg⁻¹ buried in soil with a history of atrazine application (conditioned soil), the abundance of Actinobacteria increased approximately 80-fold whereas in control soil, Actinobacteria were enriched only 10-fold and the gamma-Proteobacteria and Planctomycetes increased by 60- and 25-fold, respectively. The gamma-Proteobacteria were enriched by 120- and 230-fold in beads containing 200 mg atrazine kg⁻¹ in conditioned and control soil, respectively. The results demonstrate that BioSep^® beads are a suitable matrix for recruiting a diverse subset of the bacterial community involved in atrazine degradation.