The Nutritive Value and Yield of Alfalfa Cultivated on Sulphur Postmining Lands

The study was carried out on the reclaimed lands of a bore-hole sulphur mine. The cropping of two succeeding cuts of alfalfa (Medicago sativa L.) was determined and its chemical composition was analyzed. Alfalfa was cultivated on waste lime (flotation tailings), enriched with a humus layer. The levels of basic nutrients, total S, total F, Ca, P, Mg, K, Na, Fe and Mn were determined in alfalfa. The biomass of an average crop was 6830 kg  ha^–1 in the first cut and 14300 kg  ha^–1 in the second one. The yields of dry matter as well as of crude and true proteins were positively correlated with the phosphorus level in the dry matter of alfalfa (P < 0.01). The level of total sulphur was positively linearly correlated with the phosphorus content (P < 0.01). No harmful accumulation of sulphur or fluoride was observed. A deficiency of phosphorus in alfalfa and an abnormal Ca:P weight ratio was found. The S total level was positively correlated with N total concentration. (P < 0.01). The percentage of true protein in crude protein was negatively correlated with total sulphur (P < 0.01). The level of nonprotein nitrogen compounds was positively correlated with S total level (P < 0.01).     

The Effect of Seeding Rate and Nitrogen Fertilization on Barley Yield and Yield Components in a Cool Maritime Climate

Soil and climatic conditions in Newfoundland are on the margins of agricultural capability, and almost all feed grain is imported. The overall objective of this work was to develop guidelines for the production of barley in Newfoundland, with the goal of establishing modern cropping recommendations. We conducted a 4-year study near St. John's to examine the effect of seeding rate and topdress ammonium nitrate (N) fertilization rate on Chapais six-row barley ( Hordeum vulgare L.) yield components and grain yield. Increasing seeding rate from 200 to 380 seeds m^–2 did not alter grain yield in any year. Increasing topdress fertilization from 0 to 60 kg N ha^–1 increased spike density m^–2 at harvest, resulting in linear increases in grain yield in all years. Highest N rates had greatest lodging in two years. Based on our results, agronomic recommendations for eastern Newfoundland now include barley seeding rates of 250 ± 50 seeds m^–2, with topdress applications up to at least 30 kg N ha^–1.     

Combining Ability and Heterosis for Quantitative Powdery Mildew Resistance in Barley

The estimation of combining abilities and heterosis for quantitative resistance against Erysiphe graminis f. sp. hordei of eight spring barley (Hordeum vulgare) cultivars is presented. Fur this purpose a half diallel cross and its parents were arranged in five Latin rectangles, each inoculated with a current mildew isolate. Significant general combining ability was found whereas specific combining ability was non-significant. A great pan of the general combining ability could be explained by variety effects. Significant variety heterosis was obtained too. Significant average heterosis was obtained but us effect was small. Among those selected for this study, ‘Grit’ and ‘Hora’ were the best parents for further crosses.     

Genetic Variability for Grain Yield and Protein Content in Barley and Its Modification by Net Blotch

Protein content, yield and 1000-grain weight of healthy and net blotch-diseased plants were studied in a diallel cross programme concerning nine different varieties of barley (Hordeum vulgare L.). F₁ hybrids and their parents were grown in the field in two randomized block design experiments each with three replications (three inoculated and three non-inoculated plots). General and specific combining abilities were determined for yield, protein percentage, 1000 grain weight, protein yield, and non-protein yield. A high genetic variability for the traits studied was observed. Non-protein yield and 1000-grain weight decreased significantly in inoculated plants where protein yield was not affected. Significant general and specific combining abilities were observed for all traits. Values for general and specific combining abilities were, in some cases, significantly modified by net blotch of barley. This indicates that attention must be paid to the danger of drawing conclusions in quantitative genetics studies dealing with both diseased and healthy plants.     

The Relationships between the Nutritive Value, Yield and Resistance of Alfalfa Cultivars to Diseases

The relationships between the digestibility of organic matter, the net energy value, the gas production from in vitro incubation of tissue with rumen liquid, the quantity of yield and the resistance of a variety to disease were investigated in nine alfalfa ( Medicago sativa ) cultivars. The digestibility of organic matter and the net energy value of particular varieties were evaluated on the basis of chemical composition and the quantity of gas produced, while the resistance of cultivars to disease was evaluated visually by counting diseased plants. Negative correlations (r = - 0.85; P < 0.01) were recorded between the resistance of a variety to disease and in vitro production of gas and between the resistance of a variety to disease and net energy value of a cultivar (r = -0.78; P < 0.05) while a positive correlation (r = 0.74; P < 0.05) was established between DM yield and the disease resistance of cultivars.     

Heterosis and Genetic Variances and their Implications for Breeding Improved Varieties of Spring Beans (Vicia faba L.)*

Handcrosses were made in a factorial manner with 24 Vicia faba inbred lines m three sets, each between four male and four female parents. The resulting 48 hybrids, their parental inbreds and three commercial varieties were grown in performance trials during the years 1985—87 with three replications at one location. Yield and yield components were recorded on single plant basis. The average superiority of the hybrids above the control varieties was 26 % in plant yield. The best crosses outyielded the controls by more than 50 %. However, a few inbred lines also reached, the yield level of the controls. The average heterosis was significant for all characters except for maturity date. In plant yield the average heterosis was 75%, ranging between 34% and 148% in the various cross combinations. The highest average heterosis of 110 % was observed in yield at the lateral stems. In all characters the best inbreds were superior to the lower hybrids and in some characters even equal to the average of the hybrids. A strong positive relationship was found for all characters between hybrid performance and mid-parent value and between the per se performance of the inbreds and their general combining ability. Genotypic differences between the hybrids as well as between the inbreds were highly significant. Most of the variability between the hybrids in all characters were attributed to general combining, ability with only little evidence for specific combining ability. In the discussion the results are evaluated according to the choice of the breeding category of the partial allogamous faba bean species.     

Influence of Slight Shading, Sward Density and Nitrogen Fertilization on Yield and Nutritive Value of Lolium multiflorumo Lam.

In a two year factorial field trial the influence of slight shading (daylight reduced by 27 %), row spacing (15 and 30 cm respectively) and N fertilization (60 and 120 kg* ha^?1*cut^?1 respectively) on yield and nutritive value of Lolium multiflorum (Lam.) was investigated. Three cuts were taken in the seeding year and four in the full harvest year. Shading reduced DM yield by 4 % at low and 16 % at high level of N fertilization. Higher amount of N fertilizer increased yield by 28 % without and 12 % with shading. Row spacing was of minor importance; on average wider spacing decreased yield by 9 %. Increasing N fertilization and shading had the same effects on nutritive value: crude protein (CP), nitrate content and protein/energy ratio (P/E) went up, whereas energy value (NEL) went down. Compared to the effects of shading or N fertilization, the influence of sward density on nutritive value was small. The effect of shading and high fertilization on nutritive value was similar in both years. However for vegetatively grown forage in the seeding year shading caused P/E values and nitrate contents too high for an adequate ruminant feeding which was already evident at low level of N fertilization; also increased supply of N fertilizer without shading deteriorated the nutritive value. On the other hand forage of the full harvest year (at reproductive stages) showed too high P/E values only in shaded and highly fertilized plots. Therefore N fertilization ought to be limited at periods when the grass only grows vegetatively and/or light intensity is low, like e.g. in autumn. None of the experimental treatments had any marked effect on the fibre content of the forage (ADF) in the seeding year; in the full harvest year, however, when plants grew reproductively, shading as well as higher N fertilization increased ADF content. There was no significant correlation between ADF and NEL for vegetatively grown plants but a close relation for plants which were at reproductive stages.     

磷钾配合施肥对紫花苜蓿产量的影响

在京郊地区于2003-2005年进行了紫花苜蓿配合施肥试验。采用两因素裂区设计,以鲜草产量、干草产量、茎叶比为形状指标,测定并分析了不同磷钾肥处理对紫花苜蓿产量的影响。试验结果表明:配合施肥可显著提高苜蓿的产量(p<0.05),其中施肥处理6(45.8 kg/hm2P2O5和12.5 kg/hm2K2O)中对提高苜蓿产量的效果最为明显。2004年和2005年的鲜草产量分别为67900.5 kg/hm2和97320 kg/hm2,比对照增产19.3%和10.7%;干草产量分别为18870 kg/hm2和22999.5 kg/hm2,比对照增产22.9%和23%。方差分析表明,不同施肥处理之间鲜草产量、干草产量、茎叶比呈显著性差异(P<0.05)。     

施肥对盐碱地苜蓿产量和品质的影响

采用田间试验,研究了在盐碱土壤条件下腐植酸、磷酸二铵及微量元素配施对紫花苜蓿(Medicago sativa L.)生长的影响,并采用灰色系统理论中的灰色关联度分析法对紫花苜蓿12个主要营养指标进行综合分析和评价。结果表明:以腐植酸、磷酸二铵和微量元素为主的底肥能有效增加紫花苜蓿全年干物质产量,平均增产达到39.6%;返青期追肥对产量影响不显著。施肥均能促进紫花苜蓿株高的增长,并且第一、二茬株高均大于第三茬。不同施肥处理下,紫花苜蓿营养价值加权关联度和等权关联度的结果一致,施肥处理的优劣顺序依次为T3(底肥)、T1(底肥+返青期追肥)、T2(返青期追肥)和T4(不施肥),T3在加权和等权综合评估关联度分别为0.2175和0.2156。     

Genetics of Seed Yield and its Components in Common Bean (Phaseolus vulgaris L.) of Middle-American Origin

Genetic variance, heritability, and expected response from selection arc useful in devising alternative methods and criteria of: selection. The objectives of this study were to estimate these for seed yield and its components from 200 F₂: populations involving 80 cultivars and lines of mostly small-seeded dry bush bean (Phaseolus vulgaris L.) of habits growth I, II, and III of Middle-American origin. All cultivars and lines were crossed in eight sets of ten parents each in a Design II mating system. The F₂ populations, without parents, were evaluated in the field in a replicates-in-sets design at two locations in Colombia in 1983. Estimates of additive genetic variance were significant for yield, pods/m², seeds/pod, and seed weight. Interaction with environments was also significant. Values for nonadditive genetic variance were not significant for either yield or yield components. The estimates of narrow sense heritability, based on the F₂ population mean and unbiased by genotype x environment interaction, were 0.21 ± 0.13 for yield. 20 ± 0.13 for pods/m², 0.57 ± 0.13 for seeds/pod, and 0.74 ± 0.15 for seed weight. The expected direct response from selection of the top 20 % of F₂ populations for yield per se would result in a 4.30 % increase in yield with a correlated response of 0.21 % in seed weight. In contrast, the expected gain from direct selection for seed weight would result in a 11.76 % increase in seed weight with a, correlated gain of 0.28 % for yield. Direct selection for pods/m² would decrease yield, seeds/pod and seed weight, while direct selection for seeds/pod would reduce pods/m² and seed weight but increase seed yield by 0.37 %. Data on yield from replicated trials in the early segregating generations could be utilized for identification and selection of promising crosses and families or lines with crosses for dry bean yield improvement.     

Genetic diversity of barley cultivars grown in Spain, estimated by RFLP, similarity and coancestry coefficients

This study was conducted with the objective of characterizing the genetic variation among a representative set of 37 barley cultivars currently grown in Spain, using restriction fragment length polymorphism (RFLP) markers. Thirty-two RFLP probes, in combination with three restriction enzymes, were used to analyse polymorphism at the molecular level. Genetic distances (GD), based on RFLP band patterns, and coancestry coefficients (f), based on pedigree records, were calculated. Of the 95 clone-enzyme combinations analysed, 71 (74.7%) were polymorphic, representing 246 RFLP patterns. A cluster analysis of GD split the sample into five distinct germplasm groups that were consistent with the history of the cultivars (winter European, spring European, CIMMYT-ICARDA materials, the single cultivar ‘Dobla’ and Spanish local materials). The Spanish group was the most distinct one and had unique alleles at markers close to major loci determining phonological adaptation. The probes which best distinguished among groups were also identified. Genetic similarity estimates were moderately consistent with f (for cultivars with complete pedigrees). The implications for integration of diversity studies into breeding programmes are discussed.     

Inheritance of partial resistance to spot blotch in barley

Diallel crosses (without reciprocals) were made among 10 different barley genotypes with genetic variability for spot blotch resistance. Forty‐five F₁ hybrids and their parents were assessed for their combining abilities for the disease resistance. Three experiments, two in a growth chamber on detached leaf and seedlings tests and one in the field on adult plant stages, were undertaken using a randomized complete block design with five replicates. A mixed conidial suspension of nine virulent isolates of the pathogen was used for inoculation. Statistical analysis showed genetic variability for spot blotch resistance. Results showed that the cultivar Banteng, the Ethiopian line CI‐5791 and the Syrian line 79‐SIO‐9 had partial resistance in all experiments. General combining ability was significant, with either positive or negative values. Resistant genotypes show favourable GCA‐effects, and they could therefore be successfully used for breeding purposes.     

苜蓿种子产量及其构成因素的多样性研究

在2005-2006年连续两年在条播条件下对国内外62份苜蓿种质55个4龄苜蓿品种的种子产量及其构成因素的多样性和相关性进行了研究.结果表明所有参试的苜蓿品种间的种子产量差异比较大,生殖枝数/m2、每生殖枝花序数、每花序小花数、每花序小荚数,每小荚种子数品种间存在较大的变异,遗传多样性比较复杂.通过对两年的数据进行相关分析发现,种子产量与牛殖枝花序数在两年中相关均极显著,可以作为高种子产量品种选育的重要指标之一.实际种子产量占潜在种子产量百分比在品种之间和茬次之间均存在较大差异,而且发现两年中实际种子产量占潜在种子产量几乎均小于4%,绝大部分在1%～2%之间.研究发现千粒重与其它指标相关性不显著,国内品种千粒重表现比较大的变异,多数品种间千粒重差异较大,但国外品种间(除Jindera外)千粒重变异较小,品种间差异不显著(p>0.05).     

印水型杂交粳稻产量及产量构成因素的配合力分析

为确定新育成印水型杂交粳稻亲本的潜力,并为印水型杂交粳稻育种提供理论基础,本研究以4个不育系和4 个恢复系为亲本进行不完全双列杂交,研究产量及其构成因素的配合力。结果表明：产量性状一般配合力(GCA)方差均达到极显著水平,除结实率外,父本的一般配合力方差大于母本,单株产量、有效穗和结实率的特殊配合力(SCA)方差达到显著或极显著水平。139A和晚轮422 的GCA效应较高,产量性状以Ⅱ类为主,139A×晚轮422 和辽粳10A×晚轮422 的单株产量水平及组合SCA效应较高。父本GCA效应和组合SCA效应对产量性状的影响较大,母本GCA效应对产量性状的影响相对较小。本研究中,父本的遗传差异大于母本,对产量性状的影响也大于母本,139A和晚轮422 有较大的应用潜力。     

Effects of Nitrogen and Foliar Sulphur Interaction on Grain Yield and Yield Components in Barley

In this study, conducted from 1979 to 1986 in southern Spain, the objective was to analyze the effects of a possible interaction between soil-applied N and foliar S applied to barley (Hordeum vulgare L.) during tillering on grain yield and to identify the mechanism involved. From 1979 to 1982, we used rates of 20, 40, 60 and 80 kg a.i. N ha^?1, together with 12.5 or 25 kg foliar a.i. S ha^?1 during tillering. The results demonstrated that foliar S at both dosages acted as a partial (but not total) substitute for N, when the latter was applied at levels of 40 to 60 kg ha^?1. These effects of S did not appear to result only from a nutritive mechanism, but rather from a hormonal mechanism through the increase in ethylene biosynthesis. Therefore, during 1983 to 1986, we used 40, 60 and 80 kg a. i. N ha^?1, together with 12.5 a. i. S ha^?1 and 0.55 kg a.i. ethrel (2-chloroethyl-phosphonic acid) ha^?1. The results showed that the effects of S and ethrel on yield were practically the same. Assayed with 40 and 60 kg N ha^?1, S and ethrel acted as partial (but not total) substitutes for N, exceeding the yield of the control without S or ethrel, and equalling the yield obtained with 20 kg more of N ha^?1. The S or ethrel applied with 80 kg N ha^?1 presented an additive effect with the N. The increases in yield using S or ethrel were in all cases due to the increased final number of spikes m^?2, which was principally a consequence of the higher number of tillers formed but also a result of increased survival of tillers to form a viable spike. In addition, the positive effects of S on yield were greater the smaller the N dosage and the lower the annual yield. Finally, we present a possible mechanism of hormonal action, to explain how foliar S applied during tillering affects grain yield in barley.     

施氮量对高粱产量、品质及氮利用效率的影响

为了更好地对高粱进行氮素管理,采用盆栽试验研究了施氮量对高粱生长、籽粒产量及品质、氮素累积及转运利用的影响。选取肥力较低的土壤,设6个氮水平：0（N0）、0.05（N1）、0.1（N2）、0.2（N3）、0.4（N4）和0.6g/kg（N5）（风干土）。结果表明,N3处理干物质累积量、叶片SPAD值、籽粒产量、穗粒数及收获指数均显著高于N0和N5处理;N3处理籽粒淀粉含量低于N1处理,但淀粉产量最高;随施氮量的增加籽粒单宁含量降低,蛋白质含量增加,蛋白质总产量以N3和N4最高。随施氮量的增加叶鞘中NO₃^--N含量增加,N3处理挑旗期和穗花期叶鞘中NO₃^--N含量明显高于N0、N1和N2,但在灌浆期N0~N3处理间硝态氮含量没有显著差异;N3处理从茎叶向籽粒的转运率最高,达到76.76%。综上,适宜的施氮量有利于高粱生长及产量的提高,且在生长前期提高了叶鞘中硝态氮累积,能协调籽粒产量和功能成分的关系,获得较高的淀粉和蛋白总产量。     

我国北方不同年代玉米杂交种产量及穗部性状的遗传增益

以北方地区不同年代有代表性的玉米杂交种及其亲本为试材,对产量杂种优势的遗传增益及穗部性状杂种优势变化进行探讨.结果表明:(1)随着品种更替产量逐年提高;(2)各穗部性状随年代的推移都有不同程度的改良,单产的提高是各穗部性状协同作用的结果;(3)穗粗和籽粒深度是1990年以后杂交种产量增长的主要遗传增益;(4)产量的增长与杂种优势增加并不一致.     

施氮水平对啤酒大麦植株氮素吸收与利用及籽粒蛋白质积累和产量的影响

2004—2006年连续两个生长季,以苏啤3和单2两个啤酒大麦品种为材料,探讨施纯氮0、75、150、225和300 kg hm^-2条件下,啤酒大麦氮素积累和转运、氮素利用及籽粒产量和蛋白质积累的特性。在0~225 kg hm^-2施氮量范围内,啤酒大麦花前植株氮素积累量和转运量均随施氮水平的提高呈上升趋势,但施氮量提高至300 kg hm^-2后,提高幅度变小;而花前氮素转运效率及其对籽粒氮的贡献率则均随施氮水平提高呈单峰曲线变化。籽粒谷氨酰胺合成酶和谷-丙转氨酶活性也随着施氮水平的提高而上升,促进蛋白质积累,提高籽粒蛋白质含量,而当施氮量低于197 kg hm^-2时籽粒蛋白质含量才低于12%,符合啤酒大麦酿造要求。经回归分析,在施氮量为241 kg hm^-2时产量最高。此外,氮肥回收效率以225 kg hm^-2施氮处理为最高,氮素生理利用效率和氮收获指数随施氮量增加而显著降低。综合考虑各项指标,建议在类似本试验条件的啤酒大麦生产区,施氮量以150~197 kg hm^-2为宜。