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Masanao MATAYOSHI Takashi KITANO Tetsu SASAKI Masaji NAKAMURA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(6):705-710
A total of 349 Salmonella enterica subspecies enterica
serovar Choleraesuis (S. Choleraesuis) strains, which were isolated
between 2008 and 2012 from 349 pigs at two slaughterhouses in Okinawa Prefecture, Japan,
were investigated for antimicrobial susceptibility and the presence of antimicrobial
resistance genes. All isolates were resistant to at least four antimicrobial agents. The
antimicrobial agents for which isolates showed a high incidence of resistance were as
follows: ampicillin (100%) and streptomycin (100%), followed by gentamicin (99.7%),
oxytetracycline (99.7%), sulfamethoxazole/trimethoprim (99.4%), nalidixic acid (40.1%) and
oxolinic acid (40.1%). All isolates were sensitive to cefuroxime, ceftiofur, colistin,
fosfomycin, enrofloxacin, orbifloxacin and danofloxacin. The predominant resistance
phenotypes and genotypes were: resistance to ampicillin, streptomycin, gentamicin,
oxytetracycline and sulfamethoxazole/trimethoprim (58.5%, 204/349) and
blaTEM-strA-strB-aadA1-aadA2-aacC2-tet
(B)-sul1-sul2-dhfrXII-dhfrXIII (36.1%, 126/349). The quinolone
resistance-determining regions (QRDRs) of gyrA, gyrB, parC and
parE of the quinolone-resistant isolates (n=12) showed amino acid
substitutions of Ser-83→Phe or Asp-87→Tyr in GyrA and Ser-107→Ala in ParC. To our
knowledge, this is the first report on the molecular characterization of antimicrobial
resistance among S. Choleraesuis strains in Japan. 相似文献
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Bruno B. Chomel Jennifer B. Henn Rickie W. Kasten Nathan C. Nieto Janet Foley Sophia Papageorgiou Claire Allen Jane E. Koehler 《Veterinary research》2009,40(4)
Bartonella rochalimae was first isolated from the blood of a human who traveled to Peru and was exposed to multiple insect bites. Foxes and dogs are likely natural reservoirs for this bacterium. We report the results of experimental inoculation of two dogs, five cats and six guinea pigs with the only human isolate of this new Bartonella species. Both dogs became bacteremic for 5–7 weeks, with a peak of 103–104 colony forming units (CFU)/mL blood. Three cats had low bacteremia levels (< 200 CFU/mL) of 6–8 weeks’ duration. One cat that remained seronegative had two bacterial colonies isolated at a single culture time point. A fifth cat never became bacteremic, but seroconverted. None of the guinea pigs became bacteremic, but five seroconverted. These results suggest that dogs could be a reservoir of this strain of B. rochalimae, in contrast to cats and guinea pigs. 相似文献
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This study was conducted to investigate the presence of Escherichia (E.) coli O157 and E. coli O157:H7 and stx1 and stx2 genes on cattle carcasses and in rectal samples collected from Samsun Province of Turkey. A total of 200 samples collected from cattle carcasses and the rectal contents of 100 slaughtered cattle from two commercial abattoirs were tested using the immunomagnetic separation technique and multiplex PCR methods. E. coli O157 and E. coli O157:H7 were detected in 52 of the 200 samples (26%) tested. Of the positive samples, 49 were E. coli O157 and three were E. coli O157:H7. The E. coli O157 strain was isolated from 24 carcasses and 25 rectal samples, while E. coli O157:H7 was isolated from two carcasses and one rectal sample. Of the 49 samples positive for E. coli O157, 32 were from the rectal and carcass samples of the same animal, while two E. coli O157:H7 isolates were obtained from rectal swabs and carcasses of the same animal. The stx1 and stx2 genes were both detected in 35 E. coli O157 isolates and one E. coli O157:H7 isolate, but the stx2 gene was only detected alone in two E. coli O157 isolates. Overall, 16 carcasses tested positive for E. coli O157 and one carcass tested positive for E. coli O157:H7 based on both carcass and rectal samples. Overall, the results of this study indicate that cattle carcasses pose a potential risk to human health due to contamination by E. coli O157 and E. coli O157:H7 in the feces. 相似文献
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Shao-Kuang CHANG Dan-Yuan LO Hen-Wei WEI Hung-Chih KUO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(1):59-65
This study determined the
antimicrobial resistance profiles of Escherichia coli isolates from dogs
with a presumptive diagnosis of urinary tract infection (UTI). Urine samples from 201 dogs
with UTI diagnosed through clinical examination and urinalysis were processed for
isolation of Escherichia coli. Colonies from pure cultures were
identified by biochemical reactions (n=114) and were tested for susceptibility to 18
antimicrobials. The two most frequent antimicrobials showing resistance in Urinary
E. coli isolates were oxytetracycline and ampicillin. Among the
resistant isolates, 17 resistance patterns were observed, with 12 patterns involving
multidrug resistance (MDR). Of the 69 tetracycline-resistant E. coli
isolates, tet(B) was the predominant resistance determinant and was
detected in 50.9% of the isolates, whereas the remaining 25.5% isolates carried the
tet(A) determinant. Most ampicillin and/or amoxicillin-resistant
E. coli isolates carried blaTEM-1 genes.
Class 1 integrons were prevalent (28.9%) and contained previously described gene cassettes
that are implicated primarily in resistance to aminoglycosides and trimethoprim
(dfrA1, dfrA17-aadA5). Of the 44 quinolone-resistant
E. coli isolates, 38 were resistant to nalidixic acid, and 6 were
resistant to nalidixic acid, ciprofloxacin and enrofloxacin. Chromosomal point mutations
were found in the GyrA (Ser83Leu) and ParC (Ser80Ile) genes. Furthermore, the
aminoglycoside resistance gene aacC2, the chloramphenicol resistant gene
cmlA and the florfenicol resistant gene floR were also
identified. This study revealed an alarming rate of antimicrobial resistance among
E. coli isolates from dogs with UTIs. 相似文献
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Toru TACHIBANA Ken Takeshi KUSAKABE Sayuri OSAKI Takeshi KURAISHI Shosaku HATTORI Midori YOSHIZAWA Chieko KAI Yasuo KISO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(1):161-165
The New World monkey Aotus spp. (night monkeys) are expected
for use of valuable experimental animal with the close species of Saimiri
spp. (squirrel monkeys). Saimiri is known to show spontaneous
hypercortisolemia, although few reports in Aotus. We compared basic
states of blood steroid hormones and histological structure of the adrenal glands in two
monkeys. Serum cortisol and ACTH levels were statistically lower in Aotus
than Saimiri. Conversely, Aotus adrenocortical area
showed significant enlargement, especially at the zona fasciculata. Electron microscopic
observation at Aotus fasciculata cells revealed notable accumulation of
large lipid droplets and irregular shapes of the mitochondrial cristae. These results
suggest potential differences in cellular activities for steroidogenesis between
Aotus and Saimiri and experimental usefulness in
adrenocortical physiology and pathological models. 相似文献
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Background
The mutant prevention concentration (MPC) is an important parameter to evaluate the likelihood of growth of fluoroquinolone-resistant mutants for antimicrobial-pathogen combinations. The MPCs of fluoroquinolones for different canine pathogens have not been compared. In this study, we compared for the first time orbifloxacin MPCs between susceptible strains of Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus pseudintermedius of canine origin.Methods
More than 1010 CFU/ml of 10 strains of each bacterial species were inoculated onto Muller-Hinton agar supplemented with different concentrations of orbifloxacin from 1× to 64× minimum inhibitory concentration (MIC) and the MPCs were recorded. MICs of original strains and of mutants arising after exposure to sub-MPC concentrations (one per original strain) were determined in the presence or absence of efflux pump inhibitors (EPIs). The effects of quinolone resistance-determining region (QRDR) mutations were also examined.Results
MPCs were significantly higher for P. aeruginosa (16–128 μg/ml) than for E. coli (0.5–32 μg/ml). MPCs for S. pseudintermedius varied between the low-susceptible (16–128 μg/ml) and the high-susceptible strains (4–16 μg/ml) and were the most broadly distributed among the three species. Regarding resistance mechanisms, only one QRDR mutation in gyrA was found in all of the 10 mutants of E. coli and in 4 of the 10 mutants of P. aeruginosa, whereas mutations in both grlA and gyrA were found in 3 mutants and one mutation in grlA was found in 2 mutants among the 10 mutants of S. pseudintermedius. In the presence of an EPI, the MICs of P. aeruginosa mutants decreased markedly, those of E. coli mutants decreased moderately, and those of S. pseudintermedius mutants were unaffected.Conclusions
MPCs of orbifloxacin vary between bacterial species of canine pathogens, possibly due to the diversity of the main fluoroquinolone resistance mechanism among these species. Therefore, the type of bacterial species should be taken into consideration when using fluoroquinolone drugs such as orbifloxacin in canines. 相似文献10.
Luciana Rossi Alessia Di Giancamillo Serena Reggi Cinzia Domeneghini Antonella Baldi Vittorio Sala Vittorio Dell'Orto Annelies Coddens Eric Cox Corrado Fogher 《Journal of veterinary science (Suw?n-si, Korea)》2013,14(3):263-270
Verocytotoxic Escherichia (E.) coli strains are responsible for swine oedema disease, which is an enterotoxaemia that causes economic losses in the pig industry. The production of a vaccine for oral administration in transgenic seeds could be an efficient system to stimulate local immunity. This study was conducted to transform tobacco plants for the seed-specific expression of antigenic proteins from a porcine verocytotoxic E. coli strain. Parameters related to an immunological response and possible adverse effects on the oral administration of obtained tobacco seeds were evaluated in a mouse model. Tobacco was transformed via Agrobacteium tumefaciens with chimeric constructs containing structural parts of the major subunit FedA of the F18 adhesive fimbriae and VT2e B-subunit genes under control of a seed specific GLOB promoter. We showed that the foreign Vt2e-B and F18 genes were stably accumulated in storage tissue by the immunostaining method. In addition, Balb-C mice receiving transgenic tobacco seeds via the oral route showed a significant increase in IgA-positive plasma cell presence in tunica propria when compared to the control group with no observed adverse effects. Our findings encourage future studies focusing on swine for evaluation of the protective effects of transformed tobacco seeds against E. coli infection. 相似文献
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Maiko OHTORI Mikiko AOKI Tadashi ITAGAKI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(1):105-107
This study was designed to clarify the differences in the internal transcribed
spacer (ITS) 1 and 5.8S nucleotide sequences of Moniezia expansa,
M. benedeni and M. monardi isolated from ruminants in
Japan and to determine their phylogenetic relationships. A 98% similarity in the 5.8S
sequences was observed among the 3 Moniezia species, whereas many
nucleotide indels and substitutions were observed in the ITS1 sequences among the three
Moniezia species. These results suggest that the ITS1 region could
serve as a potential marker for discriminating the 3 Moniezia species. In
the phylogenetic tree based on the ITS1 sequences, M. monardi and
M. benedeni showed genetically closer relationship to each other than
to M. expansa. 相似文献
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Sarah Bonnet Nadine Brisseau Axelle Hermouet Maggy Jouglin Alain Chauvin 《Veterinary research》2009,40(3)
Babesia sp. (EU1), first characterized in 2003, has been implicated in human cases of babesiosis in Italy, Austria and Germany. It has been identified in roe deer and in its suspected tick vector, Ixodes ricinus, in several European countries. The aim of the present study was to validate the competence of I. ricinus as a vector of Babesia sp. (EU1) via experimental infections. For this purpose, a parasite strain isolated from roe deer was cloned in sheep erythrocytes. After experimental infections, parasite DNA was successfully amplified by PCR in both eggs and larvae originating from infected I. ricinus females and in the salivary glands of females exposed to Babesia sp. (EU1) as nymphs. We also demonstrate that infected females were able to transmit parasite DNA during a new blood meal. Together with previous epidemiological studies, these results validate I. ricinus as a competent vector for Babesia sp. (EU1). 相似文献
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Tan Duc Nguyen Thin Thanh Vo Hung Vu-Khac 《Journal of veterinary science (Suw?n-si, Korea)》2011,12(2):159-164
This study was conducted to determine the prevalence and characteristics of pathogenic Escherichia (E.) coli strains from diarrheic calves in Vietnam. A total of 345 E. coli isolates obtained from 322 diarrheic calves were subjected to PCR and multiplex PCR for detection of the f5, f41, f17, eae, sta, lt, stx1, and stx2 genes. Of the 345 isolates, 108 (31.3%) carried at least one fimbrial gene. Of these 108 isolates, 50 carried genes for Shiga toxin and one possessed genes for both enterotoxin and Shiga toxin. The eae gene was found in 34 isolates (9.8%), 23 of which also carried stx genes. The Shiga toxin genes were detected in 177 isolates (51.3%) and the number of strains that carried stx1, stx2 and stx1/stx2 were 46, 73 and 58, respectively. Among 177 Shiga toxin-producing E. coli isolates, 89 carried the ehxA gene and 87 possessed the saa gene. Further characterization of the stx subtypes showed that among 104 stx1-positive isolates, 58 were the stx1c variant and 46 were the stx1 variant. Of the 131 stx2-positive strains, 48 were stx2, 48 were stx2c, 11 were stx2d, 17 were stx2g, and seven were stx2c/stx2g subtypes. The serogroups most prevalent among the 345 isolates were O15, O20, O103 and O157. 相似文献
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Khethiwe MTSHALI Zamantungwa T. H. KHUMALO Ryo NAKAO Dennis J. GRAB Chihiro SUGIMOTO Oriel M. M. THEKISOE 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(12):1573-1579
Ticks carry and transmit a remarkable array of pathogens including bacteria, protozoa and
viruses, which may be of veterinary and/or of medical significance. With little to no
information regarding the presence of tick-borne zoonotic pathogens or their known vectors
in southern Africa, the aim of our study was to screen for Anaplasma
phagocytophilum, Borrelia burgdorferi, Coxiella
burnetii, Rickettsia species and Ehrlichia
ruminantium in ticks collected and identified from ruminants in the Eastern
Cape, Free State, KwaZulu-Natal and Mpumalanga Provinces of South Africa. The most
abundant tick species identified in this study were Rhipicephalus evertsi
evertsi (40%), Rhipicephalus species (35%), Amblyomma
hebraeum (10%) and Rhipicephalus decoloratus (14%). A total of
1634 ticks were collected. DNA was extracted, and samples were subjected to PCR
amplification and sequencing. The overall infection rates of ticks with the target
pathogens in the four Provinces were as follows: A. phagocytophilum, 7%;
C. burnetii, 7%; E. ruminantium, 28%; and
Rickettsia spp., 27%. The presence of B. burgdorferi
could not be confirmed. The findings of this study show that zoonotic pathogens are
present in ticks in the studied South African provinces. This information will aid in the
epidemiology of tick-borne zoonotic diseases in the country as well as in raising
awareness about such diseases in the veterinary, medical and tourism sectors, as they may
be the most affected. 相似文献