Types and components of resistance to Fusarium head blight of wheat

Resistance of wheat to Fusarium head blight caused by Fusarium graminearum and F. culmorum was identified in natural epidemics in 1985 and 1987 as well after artificial inoculations (1983–1988 and 1984–1987). Out of 25 genotypes tested, five were identified with no significant difference in head blight scores, but differing significantly in yield after artificial inoculation, i.e. tolerance differences were detected at different resistance levels. Some genotypes that were similar in yield or head blight scores differed in seed infection severity. Genotypes with awns were more susceptible to head blight when tested under natural epidemic condition in the field; but this trait did not influence head blight severity in artificial inoculations. Dwarf genotypes were more severely infected by head blight than tall genotypes under natural conditions, but genotypes of different plant height classes were similarly susceptible after artificial inoculations. In the early generations of a breeding programme resistance measured by visual evaluation of artificial inoculation is the most important way to screen. If selection of dwarf and awned genotypes cannot be avoided, the higher susceptibility caused by awns and dwarfness under natural epidemic conditions can be decreased by a higher level of physiological resistance, as variability in physiological resistance is available. In later generations, traits like percentage of seed infection or tolerance can be identified by additionally measuring yield reduction. Stability of disease reaction appears to be connected with resistance level, the most resistant genotypes are the most stable, and the most susceptible ones tend to have more unstable reactions in different epidemic conditions.     

Different quantitative trait loci for <Emphasis Type="Italic">Fusarium</Emphasis> resistance in wheat seedlings and adult stage in the Wuhan/Nyubai wheat population

Fusarium head blight (FHB), caused primarily by Fusarium graminearum (Schwabe), is an important wheat disease. In addition to head blight, F. graminearum also causes Fusarium seedling blight (FSB) and produces the mycotoxin deoxynivalenol (DON) in the grain. The objectives of this study were: (1) to compare the relationship between resistance of wheat lines to F. graminearum in the seedlings and spikes and (2) to determine whether the quantitative trait loci (QTL) for FSB were the same as QTLs for FHB resistance and DON level reported for the same population previously (Somers et al. 2003). There was no relationship between FSB infection and FHB index or DON content across the population. A single QTL on chromosome 5B that controlled FSB resistance was identified in the population; the marker WMC75 explained 13.8% of the phenotypic variation for FSB. This value implies that there may be other QTL with minor effects present, but they were not detected in the analysis. Such a QTL on chromosome 5B was not reported previously among the QTLs associated with FHB resistance and DON level in this population. However, because of recombination, some lines in the present study have Fusarium resistance for both seedling and head blight simultaneously. For example, DH line HC 450 had the highest level of resistance to FSB and FHB and was among the ten lines with lowest DON content. This line is a good candidate to be used as a parent for future crosses in breeding for Fusarium seedling resistance, together with breeding for head blight resistance. This approach may be effective in increasing overall plant resistance to Fusarium.     

Genetic variation for head blight resistance in triticale caused by Fusarium graminearum isolates of different deoxynivalenol production

Summary Fusarium head blight infection causes severe yield losses and contamination of the grain with mycotoxins in triticale (× Triticosecale Wittmack) grown in temperate and semihumid areas. In a two-year experiment thirty-six genotypes were inoculated separately with two isolates of Fusarium graminearum differing fivefold in their in vitro deoxynivalenol (DON) production and the effect on various traits was studied. All traits were significantly affected by head blight. The two isolates differed considerably in their aggressiveness resulting in a mean reduction of grain weight per spike of almost 25% and 50%, respectively. Inter-annual correlation was high for average disease rating (r=0.63, P<-0.01) and low for the other traits. Therefore, disease rating, averaged from two to three records, was regarded a suitable criterion for screening purposes. The effect of isolates on genotypes was not stable over years. The mean DON content of five genotypes with diverse resistance levels was 68 mg kg^-1. In vitro DON production of the two isolates used for inoculation did not correspond to their aggressiveness and DON contamination of the grain.     

Breeding wheat and rye for resistance to Fusarium diseases

Fusarium culmorum and F. graminearum Groups 1 and 2 cause seedling blight, crown rot, foot rot and head blight in wheat and rye that may affect grain yield and quality for baking and feeding. This review starts with an analysis of Fusarium populations with regard to their genetic variation for aggressiveness, mycotoxin production, and isolate-by-host genotype interaction. To assess resistance in the different host growth stages, quantitative inoculation and disease assessment techniques are necessary. Based on estimated population parameters, breeding strategies are reviewed to improve Fusarium resistance in wheat and rye. Epidemiological and toxicological aspects of Fusarium resistance that are important for resistance breeding are discussed. F. culmorum and F. graminearum display large genetic variation for aggressiveness in isolate collections and in naturally occurring populations. The production of mycotoxins, especially deoxynivalenol and its derivatives, is a common trait in these populations. Significant isolate-by-host genotype interactions were not found across environments in wheat and rye. Artificial infections in the field are indispensable for improving Fusarium crown rot, foot rot and head blight resistance in wheat and rye. For a reliable disease assessment of large populations, disease severity ratings were found to be the most convenient. The differentiation of host resistance is greatly influenced by an array of nongenetic factors (macro-environment, microclimate, host growth stage, host organ) that show significant interactions with host genotype. Selection for environmentally stable resistance has to be performed in several environments under a maximum array of different infection levels. Selection in early growth stages or on one plant organ does not in most cases allow prediction of resistance in adult-plant stages or another plant organ. Significant genetic variation for resistance exists for all Fusarium-incited diseases in breeding populations of wheat and rye. The patho-systems studied displayed a prevalence of additive gene action with no consistent specific combining ability effects and thus rapid progress can be expected from recurrent selection. In wheat, intensive testing of parental genotypes allows good prediction of the mean head blight resistance after crossing. Subsequent selection during selfing generations enables the use of transgression towards resistance. In hybrid breeding of winter rye, the close correlation between foot rot resistance of inbred lines and their GCA effects implies that selection based on the lines per se should be highly effective. This is not valid for F. culmorum head blight of winter rye caused by a greater susceptibility of the inbred lines compared to their crosses. For both foot rot and head blight resistance, a high correlation between the resistance to F. graminearum and F. culmorum was found in wheat and rye. Mycotoxin accumulation occurs to a great extent in naturally and artificially infected plant stands. The correlation between resistance traits and mycotoxin contents are medium and highly dependent on the environment. Further experiments are needed to clarify whether greater resistance will lead to a correlated reduction of the mycotoxin content of the grains under natural infection.     

Effects of genotype and genotype—environment interaction on deoxynivalenol accumulation and resistance to Fusarium head blight in rye, triticale, and wheat

Fusarium culmorum is one of the most important Fusarium species causing head blight infections in wheat, rye, and triticale. It is known as a potent mycotoxin producer with deoxynivalenol (DON), 3‐acetyl deoxynivalenol (3‐ADON), and nivalenol (NIV) being the most prevalent toxins. In this study, the effect of winter cereal species, host genotype, and environment on DON accumulation and Fusarium head blight (FHB) was analysed by inoculating 12 rye, eight wheat, and six triticale genotypes of different resistance levels with a DON‐producing isolate at three locations in 2 years (six environments). Seven resistance traits were assessed, including head blight rating and relative plot yield. In addition, ergosterol, DON and 3‐ADON contents in the grain were determined. A growth‐chamber experiment with an artificially synchronized flowering date was also conducted with a subset of two rye, wheat and triticale genotypes. Although rye genotypes were, on average, affected by Fusarium infections much the same as wheat genotypes, wheat accumulated twice as much DON as rye. Triticale was least affected and the grain contained slightly more DON than rye. In the growth‐chamber experiment, wheat and rye again showed similar head blight ratings, but rye had a somewhat lower relative head weight and a DON content nine times lower than wheat (3.9 vs. 35.3 mg/kg). Triticale was least susceptible with a five times lower DON content than wheat. Significant (P = 0.01) genotypic variation for DON accumulation existed in wheat and rye. The differences between and within cereal species in the field experiments were highly influenced by environment for resistance traits and mycotoxin contents. Nevertheless, mean mycotoxin content of the grain could not be associated with general weather conditions in the individual environments. Strong genotype‐environment interactions were found for all cereal species. This was mainly due to three wheat varieties and one rye genotype being environmentally extremely unstable. The more resistant entries, however, showed a higher environmental stability of FHB resistance and tolerance to DON accumulation. Correlations between resistance traits and DON content were high in wheat (P = 0.01), with the most resistant varieties also accumulating less DON, but with variability in rye. In conclusion, the medium to large genotypic variation in wheat and rye offers good possibilities for reducing DON content in the grains by resistance selection. Large confounding effects caused by the environment will require multiple locations and/or years to evaluate FHB resistance and mycotoxin accumulation.     

Genetic variation for resistance to Fusarium head blight in bread wheat

Summary During a four year period, a total of 258 winter and spring wheat genotypes were evaluated for resistance to head blight after inoculation with Fusarium culmorum strain IPO 39-01. It was concluded that genetic variation for resistance is very large. Spring wheat genotypes which had been reported to be resistant to head blight caused by Fusarium graminearum were also resistant to F. culmorum. The resistant germplasm was divided into three gene pools: winter wheats from Eastern Europe, spring wheats from China/Japan and spring wheats from Brazil. In 32 winter wheat genotypes in 1987, and 54 winter wheat genotypes in 1989, the percentage yield reduction depended on the square root of percentage head blight with an average regression coefficient of 6.6. Heritability estimates indicated that for selection for Fusarium head blight resistance, visually assessed head blight was a better selection criterion than yield reduction.     

Resistance to Fusarium head blight and deoxynivalenol accumulation in wheat

Fusarium head blight (FHB), caused by Fusarium graminearum Schwabe (telomorph =Gibberella zeae (Schw.)), is an important wheat disease world‐wide. Production of deoxynivalenol (DON) by F. graminearum in infected wheat grain is detrimental to livestock and is also a safety concern in human foods. An international collection of 116 wheat lines was evaluated for FHB resistance and concentration of DON in grain. Plants were inoculated with mixed isolates of F. graminearum in the greenhouse by injecting conidia into a single spikelet of each spike and in the field by scattering F. graminearum‐infected wheat kernels on the soil surface. FHB symptoms were evaluated by visual inspection in both the greenhouse and field, and DON was analysed by HPLC. Significant differences in FHB ratings and DON levels were observed among cultivars. In the greenhouse test, visual symptoms varied from no spread of FHB from the inoculated spikelet to spread throughout the spike, and DON levels ranged from trace levels to 283 mg/kg. In the field test, DON ranged from 2.8 to 52 mg/kg. The greenhouse test identified 16 wheat lines from various origins that accumulated less than 2 mg/kg DON. These lines may be useful as sources for breeding wheat cultivars with lower DON levels. Correlation coefficients were significant between FHB symptom ratings, seed quality traits, and DON levels. Thus, the percentage of scabbed spikelets and kernels can be generally used to predict DON levels in harvested wheat grain. In breeding programmes, selection for plants having few scabbed spikelets and scabbed kernels is most likely to result in low DON levels.     

Common resistance of wheat to members of the Fusarium graminearum species complex and F. culmorum

Fusarium head blight (FHB) is the most important disease of wheat in Central Europe. Although common resistance of wheat against several Fusarium species has been proposed recently, no data were available for the recently described species/lineages of F. graminearum and F. culmorum . In this study, twenty wheat genotypes were tested under field conditions by spraying inocula of isolates of eight species of the F. graminearum species complex, and three lineages of F. culmorum in 2003–2004. The severity of FHB, Fusarium damaged kernels, yield reduction and deoxynivalenol/nivalenol contamination were measured. F. culmorum isolates were in general more aggressive to wheat than those belonging to the F. graminearum species complex. The various wheat genotypes exhibited similar reactions against the different Fusarium isolates, indicating that resistance to F. graminearum sensu stricto was similar to that for the other species of the F. graminearum species complex examined. This is an important message to breeders as the resistance relates not only to any particular isolate of F. graminearum , but similarly to isolates of other Fusarium species.     

Genotypic and environmental variation of resistance to head blight in triticale inoculated with Fusarium culmorum

Fusarium head blight (FHB) is a widespread disease of small‐grain cereals and can cause substantial losses in grain yield. To assess quantitative genetic parameters as a basis for an efficient breeding programme for resistance, 100 triticale (×Triticosecale Wittm.) genotypes were tested in various environments and artificially inoculated at anthesis with an aggressive isolate of Fusarium culmorum. A visual rating (1–9 scale) was used to assess head blight infection. Five grain yield traits relative to an uninoculated control were also measured. The mean value of the average rating, calculated from four or five readings, was 4.4. It ranged from 3.0 to 5.9 and showed continuous variation. Infection caused a 48% reduction of mean kernel weight per spike, which was the result of 26% fewer kernels per spike and a 32% lower 1000‐kernel weight. The 50‐ml kernel weight was affected by only 20%. The range and genotypic variation was highest for relative kernel weight per spike. For all relative grain yield traits, the most important source of variation was the environment, followed by genotype‐environment interaction, with genotype generally coming last. In contrast, genotypic variation was the most important factor for the disease rating, which also had the highest heritability (h²= 0.89). Phenotypic correlations between the average head blight rating and relative grain yield traits were moderate (r = 0.42–0.57). In conclusion, an average disease rating provides a quantitative assessment of resistance and is suitable for screening large numbers of genotypes. Relative kernel weight per spike gives a ranking of the genotypes that is very similar to the visual score.     

Comparison of rye,triticale, durum wheat and bread wheat genotypes for Fusarium head blight resistance and deoxynivalenol contamination

Small-grain winter cereal crops can be infected with Fusarium head blight (FHB) leading to mycotoxin contamination and reduction in grain weight and quality. Although a number of studies have investigated the genetic variation of genotypes within each small-grain cereal, a systematic comparison of the winter crops rye, triticale, durum and bread wheat for their FHB resistance, Fusarium-damaged kernels (FDK) and deoxynivalenol (DON) contamination across species is still missing. We have therefore evaluated twelve genotypes each of four crops widely varying in their FHB resistance under artificial infection with one DON-producing F. culmorum isolate at constant spore concentrations and additionally at crop-specific concentrations in two environments. Rye and triticale were the most resistant crops to FHB followed by bread and durum wheat at constant and crop-specific spore concentrations. On average, rye accumulated the lowest amount of DON (10.08 mg/kg) in the grains, followed by triticale (15.18 mg/kg) and bread wheat (16.59 mg/kg), while durum wheat had the highest amount (30.68 mg/kg). Genotypic variances within crops were significant (p ≤ .001) in most instances. These results underline the differing importance of breeding for FHB resistance in the different crops.     

Selection for Fusarium head blight resistance in early generations reduces the deoxynivalenol (DON) content in grain of winter and spring wheat

Fusarium head blight (FHB) results in yield losses and contamination of kernels by mycotoxins, particularly deoxynivalenol (DON). For minimizing DON content in grain, indirect selection methods would increase gains from selection compared to the costly and time‐consuming DON analysis. The aim of this study was to examine whether an early selection for fewer FHB symptoms would lead to a reduced DON content in grain after inoculation with Fusarium culmorum. Starting with a double‐cross derived population of about 1,100 genotypes, 30 F_1:3 genotypes were selected for FHB rating in a two‐step selection in spring wheat with the non‐adapted resistance sources CM82036 and ‘Frontana’. In winter wheat, 30 F_1:2 genotypes were selected out of a double‐cross derived population of about 600 F₁ plants from crosses with German resistance sources (‘Dream’, G16‐92). Selected genotypes were grouped in three categories according to their FHB rating (low, moderate and high) and analysed afterwards for grain DON content. The three groups differed in their DON content illustrating that indirect selection should already be feasible in the earliest generations. Because of the wide genotypic ranges for DON contents within one grouping, a final DON analysis for selected materials is advisable to achieve full selection gain.     

Stability of maize resistance to the ear rots caused by Fusarium graminearum and F. verticillioides in Argentinian and Canadian environments

Sources of resistance to Fusarium spp. are needed to develop maize hybrids resistant to the accumulation of fungal mycotoxins in the grain. In a search for resistant germplasm in 1999 and 2000, a set of Argentinian maize populations was evaluated in Ottawa, Canada, for resistance to ear rots after inoculation with local isolates of Fusarium verticillioides and F. graminearum. Sixteen of these populations, varying in observed resistance levels, were re-evaluated in 2003 and 2004 in Pergamino, Argentina, using local isolates of the same fungi. Conidial suspensions of each fungal species were inoculated into the silk channel of primary ears. Disease severity was assessed after physiological maturity using a scale based on the percentage of visibly infected kernels. Genotype effect was more important than genotype-by-fungal species or genotype-by-fungal species-by-environment interaction effects. In addition, disease severity levels associated with each fungal species were positively correlated (P < 0.05) (r = 0.90, r = 0.81, r = 0.87 and r = 0.53, in Ottawa 1999 and 2000, and Pergamino 2003 and 2004, respectively). Populations ARZM 01107, ARZM 07138, ARZM 10041, ARZM 13031, ARZM 16002 and Pora INTA exhibited the highest and most stable resistance to both species. Considering that disease resistance exhibited low specificity to the environment and to the fungal species in evaluations conducted in a wide range of environments and with fungal isolates collected from different hemispheres, the most resistant populations are potential sources of genes for stable resistance to these Fusarium spp.     

Reaction of triticale,wheat and rye accessions to graminaceous Fusarium spp. infection at the seedling and adult plant growth stages

Summary Pathogenicity of 20 isolates of 12 Fusarium species recovered from triticale seed against seedlings of 14 varieties of winter cereals (triticale, wheat, and rye) was tested. The most pathogenic inoculum was a mixture of isolates (a composite isolate) of all the species. The following species were individually the most pathogenic: F. avenaceum, F. culmorum, F. sambucinum var. coeruleum, and F. graminearum. Winter triticale was more resistant to seedling blight than rye but more susceptible than wheat.Also reactions of 31 winter and 12 spring varieties of cereals to head inoculation with a composite isolate of 4 Fusarium spp. (F. avenaceum, F. culmorum, F. graminearum, and F. sambucinum var. coeruleum) was studied. In comparison to other cereals of similar type winter and spring wheat appeared to be the most susceptible while winter rye reaction was comparable to winter triticale. Spring and winter triticale varieties responded to head infection intermediately.There was no significant correlation between seedling and head reactions to infection with Fusarium spp. for winter rye and triticale. For winter wheat a negative trend was found. The above findings imply that screening of cereals at the seedling stage can not be used to predict the resistance to head blight. Nevertheless, resistance at the stage is highly desirable to prevent excessive damage of the crops due to the seedling blight incited by Fusarium spp..     

Correlations among Fusarium culmorum head blight resistance, fungal colonization and mycotoxin contents in winter rye

Fusarium culmorum head blight infections may lead to accumulation of toxic metabolites in winter rye grain. To estimate the correlation between resistance traits, fungal colonization and accumulation of deoxynivalenol (DON), 3-acetyldeoxynivalenol (3-AcDON) and zea-ralenone (ZEA), 27 winter rye single-cross hybrids were artificially inoculated in 1992 and 1993. Resistance traits were head blight rating and grain weight of the inoculated relative to the non-inoculated plots. Fungal colonization was determined by the analysis of ergosterol (ERG) content in the grain. Head blight rating and relative grain yield showed a medium to high disease severity and ERG indicated a considerable fungal colonization of the kernels with a mean of 85 mg/kg in 1992 and 66 mg/kg in 1993. DON content among genotypes ranged from 0.7–28 mg/kg in 1992 and from 11 to 35 mg/kg in 1993. 3-AcDON and ZEA contents were low in both years with overall means of 1.1 and 0.09 mg/kg, respectively. Across both years, considerable genotypic variation was found for head blight rating, relative grain weight, and ERG content with medium to high heritabilities (0.6–0.7). For the mycotoxin contents, however, genotype-year interaction variance was the most important source of variation. The correlations between relative grain weight and DON, 3-AcDON, or ZEA were low in 1992 (r ～ 0.3), but considerably higher in 1993 (r ～ 0.7, P = 0.01). In contrast, correlation between relative grain weight and ERG was significant in both years (r ～ 0.5, P = 0.01). In F. culmorum head blight infections, DON, 3-AcDON and ZEA contents appear to be affected, at least partially, by different environmental factors than resistance traits and fungal colonization.     

Selection of Head Blight Resistant Wheats Through Improved Seedling Resistance

In a four years' test (1982—1985) it was shown that there are highly significant differences in resistance to seab and seedling blight in wheat. Using a novel approach, that head blight resistance should be measured on stands free of leaf diseases and with a number of isolates with different pathogenicity in the inoculation tests, the rep-rod Liability of results increased significantly over the levels so far published. The genotype- by-year and the genotype-by-isolate interactions remained low. Beside differences in resistance deviations in tolerance were also detected. The best materials that were selected are near to the better spring wheat resistance sources. A tendency for correlation between seedling and head blight resistance was found and the most resistant genotypes in the seedling stage will, with high probability, yield head blight resistant material. It thus became possible to include seedling resistance into the breeding program for head blight resistance, Further evidence for there being an identical genetic basis for resistance to F. graminearum and F. culmorum is presented.     

Effect of 3B, 5A and 3A QTL for Fusarium head blight resistance on agronomic and quality performance of Canadian winter wheat

The objectives of this study were to investigate (i) the correlations between Fusarium head blight (FHB) index, deoxynivalenol (DON) accumulation and percentage of Fusarium‐damaged kernels (FDK) with agronomic and quality traits and (ii) the effect associated with the presence of single QTLs for FHB resistance on agronomic and quality traits in winter wheat. The population was derived from the cross between ‘RCATL33' (FHB resistance derived from ‘Sumai 3’ and ‘Frontana’) and ‘RC Strategy’. Parental lines and recombinant inbred lines (RILs) were genotyped with SSR markers associated with the 3B, 5A and 3A QTLs. The population was planted in FHB‐inoculated nurseries and in agronomy trials. Lines in the 3B QTL class had the lowest FHB index, DON content and FDK level and did not have a significantly lower yield, thousand kernel weight or protein content compared with the lines grouped in other QTL classes (including no QTL class). Marker‐assisted selection of the 3B QTL for FHB resistance into high‐yielding FHB‐susceptible winter wheat is the recommended approach for the development of lines with increased FHB resistance without significant yield and quality penalties.     

Differential influence of QTL linked to Fusarium head blight,Fusarium-damaged kernel,deoxynivalenol contents and associated morphological traits in a Frontana-derived wheat population

The genetic background of Fusarium head blight (FHB) resistance in the moderately resistant wheat variety Frontana was investigated in the GK Mini Manó/Frontana DH population (n = 168). The plant material was evaluated across seven epidemic environments for FHB, Fusarium-damaged kernel (FDK) and deoxynivalenol (DON) contents caused by two Fusarium species (F. culmorum and F. graminearum). The effects of phenotypic traits such as plant height and heading date were also considered in the experiments. In the population, 527 polymorph markers (DArT, SSR) within a distance of 1,381 cM distance were mapped. The quantitative trait locus/loci (QTL) on chromosomes 4A and 4B demonstrated a significant linkage only with FHB, while QTL on chromosomes 3A, 4B, 7A and 7B were linked to DON accumulation alone. Regions determining all the investigated Fusarium resistance traits were identified on chromosomes 1B, 2D, 3B, 5A, 5B and 6B. The markers in these regions are of the greatest significance from the aspect of resistance breeding. Our results indicate that the genetic background of resistance against FHB, FDK and DON accumulation can differ, and all these traits should be taken under consideration during resistance tests. Moreover, this is the first report on the mapping of Frontana-derived QTL that influence DON accumulation, which is important since the level of DON contamination determines the actions of the food and feed industries. Selection should therefore also focus on this trait by using molecular markers linked to DON content.     

Validation of two major quantitative trait loci for fusarium head blight resistance in Chinese wheat line W14

Identity of quantitative trait loci (QTL) governing resistance to fusarium head blight (FHB) initial infection (type I), spread (type II), kernel infection, and deoxynivalenol (DON) accumulation was characterized in Chinese wheat line W14. Ninety‐six double‐haploid lines derived from a cross of W14 × ’Pion2684’ were evaluated for FHB resistance in two greenhouse and one field experiment. Two known major QTL were validated on chromosomes 3BS and 5AS in W14 using the composite interval mapping method. The 3BS QTL had a larger effect on resistance than the 5AS QTL in the greenhouse experiments, whereas, the 5AS QTL had a larger effect in the field experiment. These two QTL together explained 33%, 35%, and 31% of the total phenotypic variation for disease spread, kernel infection, and DON concentration in the greenhouse experiments, respectively. In the field experiment, the two QTL explained 34% and 26% of the total phenotypic variation for FHB incidence and severity, respectively. W14 has both QTL, which confer reduced initial infection, disease spread, kernel infection, and DON accumulation. Therefore, marker‐assisted selection (MAS) for both QTL should be implemented in incorporating W14 resistance into adapted backgrounds. Flanking markers Xbarc133 and Xgwm493 on 3BS and Xbarc117 and Xbarc56 on 5AS are suggested for MAS.     

Field evaluation of resistance to kernel infection and mycotoxin accumulation caused by Fusarium head blight in western Japanese wheat (Triticum aestivum L.) cultivars

Fusarium head blight (FHB) remains a serious problem that causes yield and grain quality losses, and mycotoxin accumulation in wheat production in western Japan. A 3-year field trial with artificial FHB inoculation was conducted to evaluate varietal characteristics of FHB resistance among 31 wheat cultivars/lines cultivated in western Japan, including one standard line. Severity of FHB, frequency of Fusarium-damaged kernels (FDK), deoxynivalenol concentration (DON), nivalenol concentration (NIV), and grain yield showed significant differences among years and among cultivars/lines. Interaction between years and cultivars/lines was also significant in these traits, but F values were larger for cultivars/lines than for the interaction. Correlation analysis showed that cultivars/lines with lower FHB severities tended to have lower FDK, DON and NIV, and a higher yield. Resistance to kernel infection (RKI), residuals calculated by regressing FDK against FHB severity, and resistance to mycotoxin accumulation (RTA), residuals calculated by regressing DON + NIV against FDK, also differed significantly among cultivars/lines. These results indicated that varietal differences in response to FHB symptom development, RKI and RTA exist among wheat cultivars/lines in western Japan. Such information is important to aid producers in controlling the disease and for breeders to improve FHB resistance and reduce mycotoxin accumulation in commercial wheat cultivars.     

Whitened kernel surface: A fast and reliable method for assessing Fusarium severity on cereal grains by digital picture analysis

Fusarium head blight (FHB) is a cereal disease of major importance responsible for yield losses and mycotoxin contaminations in grains. Here, we introduce a new measurement approach to quantify FHB severity on grains based on the evaluation of the whitened kernel surface (WKS) using digital image analysis. The applicability of WKS was assessed on two bread wheat and one triticale grain sample sets (265 samples). Pearson correlation coefficients between Fusarium‐damaged kernels (FDK) and WKS range from r = 0.77 to r = 0.81 and from r = 0.61 to r = 0.86 for the correlation between deoxynivalenol (DON) content and WKS. This new scoring method facilitates fast and reliable assessment of the resistance to kernel infection and shows significant correlation with mycotoxin content. WKS can be automated and does not suffer from the “human factor” inherent to visual scorings. As a low‐cost and fast approach, this method appears particularly attractive for breeding and genetic analysis of FHB resistance where typically large numbers of experimental lines need to be evaluated, and for which WKS is suggested as an alternative to visual FDK scorings.