早播及耕作措施对黄土高原半干旱区春小麦土壤氮磷元素的影响

为探讨早播及耕作措施对农田生态系统土壤氮磷元素影响规律,对黄土高原半干旱区早播、正常播下不同耕作措施[传统耕作(T)、传统耕作+秸秆还田(TS)、免耕(NT)、免耕+秸秆覆盖(NTS)]春小麦(Triticum aestivum)生育期0～40 cm土壤进行分析,以正常播种传统耕作春小麦为对照,研究早播及耕作措施对春小麦土壤氮磷元素的影响。结果表明:(1)不同处理下春小麦土壤N素(TN、NO_3~--N、NH+4-N)、P素(TP)含量均具有表聚性特征,其土壤N∶P均值(1.713)低于全国平均值(5.2),同时其变异水平系数处于26.99%～77.28%,属中等变异水平;(2)全生育期0～40 cm土层,早播春小麦土壤TN、NH+4-N含量及N∶P均值显著低于正常播23.1%～32.5%,不利于土壤TN、NH+4-N含量积累,而NO_3~--N含量及NO_3~--N∶N均值显著高于正常播30.5%、41.5%,有助于土壤NO_3~--N含量的固存,同时早播处理下土壤TP含量整体高于正常播处理;(3)秸秆覆盖措施(NTS、TS)下土壤TN、TP含量高于NT、T,但速效氮(NO_3~--N、NH_4~+-N)养分含量表现相反;(4)不同处理下春小麦土壤TP含量在生育期间趋于稳定,而N素含量在分蘖期及开花期变化较显著。因此,选择适宜播种时间及耕作管理措施有助于提高该区作物土壤N、P元素的积累转化效率,加快发展黄土高原半干旱区农田生态系统恢复进程。     

土壤强还原消毒过程中产生气体对土传病原菌的抑制作用

土壤强还原(reductive soil disinfestation,RSD)是一种高效、环保的土壤消毒方法,为明确RSD杀菌机理,采用气相色谱和荧光定量PCR等技术研究RSD过程中产生的气体对土传病原菌的杀菌作用。结果表明:RSD过程中土壤能够产生H_2S和NH_3;当在装有25 g病土的三角瓶中分别加入25 m L的H_2S和NH_3时,土壤中尖孢镰刀菌的数量均显著下降,其杀菌效果分别为90.3%和100.0%;当在病土中加入0.125%(w/w)氨水时,尖孢镰刀菌、茄劳尔氏菌、辣椒疫霉和立枯丝核菌的数量分别下降至对照土壤的1.0%、0.3%、0.1%和0.9%;此外,加入10μL氨水即可显著抑制尖孢镰刀菌菌丝生长和孢子萌发,抑制率分别为52.8%和100.0%。表明RSD过程中产生的H_2S和NH_3对RSD过程的杀菌效果起着一定的作用。     

Accumulation of soil organic carbon during natural restoration of desertified grassland in China’s Horqin Sandy Land

China's Horqin Sandy Land,a formerly lush grassland,has experienced extensive desertification that caused considerable carbon(C) losses from the plant-soil system.Natural restoration through grazing exclusion is a widely suggested option to sequester C and to restore degraded land.In a desertified grassland,we investigated the C accumulation in the total and light fractions of the soil organic matter from 2005 to 2013 during natural restoration.To a depth of 20 cm,the light fraction organic carbon(LFOC) storage increased by 221 g C/m2(84%) and the total soil organic carbon(SOC) storage increased by 435 g C/m2(55%).The light fraction dry matter content represented a small proportion of the total soil mass(ranging from 0.74% in 2005 to 1.39% in 2013),but the proportion of total SOC storage accounted for by LFOC was remarkable(ranging from 33% to 40%).The C sequestration averaged 28 g C/(m2·a) for LFOC and 54 g C/(m2·a) for total SOC.The total SOC was strongly and significantly positively linearly related to the light fraction dry matter content and the proportions of fine sand and silt+clay.The light fraction organic matter played a major role in total SOC sequestration.Our results suggest that grazing exclusion can restore desertified grassland and has a high potential for sequestering SOC in the semiarid Horqin Sandy Land.     

土壤强还原过程产生的有机酸对土传病原菌的抑制作用

土壤强还原(reductive soil disinfestation,RSD)能有效杀灭土传病原菌,并已在日本、荷兰和美国取得一系列的推广应用,但对于RSD的杀菌机理不甚明了。本试验采用高效液相色谱和荧光定量PCR等技术研究了以玉米秸秆为有机碳源的RSD处理过程中土壤产生的有机酸种类,以及不同浓度的有机酸对土壤病原菌的抑制效果。结果表明:以玉米秸秆为有机碳源的RSD过程中主要产生了乙酸和丁酸;经50mmol/L丁酸溶液处理的土壤中立枯丝核菌、辣椒疫霉、尖孢镰刀菌及茄劳尔氏菌的数量分别为对照的3.5%、38.9%、11.5%和7.9%;10mmol/L的丁酸可以完全抑制尖孢镰刀菌菌丝的生长;5mmol/L的丁酸即可完全抑制尖孢镰刀菌的孢子萌发。本研究结合前人报道表明有机酸,尤其是乙酸和丁酸,对于RSD的杀菌机理起着重要的作用。     

耕作方式对旱地麦田土壤团聚体及其碳氮组分分布的影响

为明确蓄水保墒耕作方式下旱地麦田土壤团聚体稳定性、有机碳及碳组分和全氮及氮组分在不同粒径团聚体组分中的分布特征,深入了解不同耕作方式下土壤碳氮固持机制,以连续3 a(2017—2020年)实施不同耕作方式(免耕、深松、深翻)后冬小麦收获期0～20 cm土壤为研究对象,采用湿筛法测算土壤团聚体的构成与稳定性(R_0.25,>0.25 mm团聚体含量;MWD,平均重量直径;GMD,几何平均直径),并测定各粒径团聚体有机碳(SOC)和碳组分(重组有机碳,HFOC;轻组有机碳,LFOC;易氧化有机碳,EOC;可溶性有机碳,DOC;颗粒有机碳,POC)含量、全氮(TN)和氮组分(硝态氮,NO^-₃-N;铵态氮,NH⁺₄-N;可溶性有机氮,SON)含量,分析了碳氮组分的相关关系。结果显示：(1)免耕和深松处理>2 mm团聚体土壤比例较深翻处理分别提高8.8%和22.1%,免耕有利于增加<0.053 mm粉黏粒比例,较深松和深翻处理分别提高46.4%和27.7%。深松处理较深翻...     

香蕉—水稻轮作联合添加有机物料防控香蕉枯萎病研究

香蕉枯萎病是一种由尖孢镰刀菌引起的土传病害, 枯萎病的发生对香蕉产业造成严重的冲击本文针对香蕉枯萎病难以防控的难题, 采用水稻轮作同时添加有机物料（椰糠、稻秆和桉树皮）的方法, 研究了其对香蕉枯萎病高发病蕉园土壤中尖孢镰刀菌(FOC)和其他微生物数量的影响及其对香蕉枯萎病的防控效果。结果表明, 轮作水稻可以显著减少土壤中FOC的数量, 从而降低香蕉枯萎病的发病率。其中轮作水稻处理比未淹水未种植水稻处理FOC的数量下降了71.5%, 下茬香蕉枯萎病发病率降低了81.7%; 与未种植水稻但淹水的处理相比FOC数量下降了47.8%, 下茬香蕉枯萎病发病率降低了71.2%; 种植水稻同时添加水稻秸秆能够显著增强病原菌的杀灭效果和对下茬香蕉枯萎病的防控效果, 相比未添加物料轮作水稻处理, 尖孢镰刀菌数量下降了36.2%, 下茬香蕉枯萎病发病率降低了50.0%。同时, 水稻轮作同时添加有机物料处理及其下茬香蕉的种植, 对土壤中可培养细菌、真菌和放线菌数量均具有不同的影响。其中水稻种植期间不同处理的可培养真菌与放线菌数量随着时间的增加整体呈下降趋势, 而在种植香蕉后随时间的增加呈上升趋势; 土壤中可培养细菌的数量在水稻种植与香蕉种植期间随着时间的增加未呈现出规律性。结论：水稻轮作联合稻秆的添加能有效降低土壤中FOC的数量和下茬香蕉枯萎病的发病率。     

Effects of long-term fertilization on oxidizable organic carbon fractions on the Loess Plateau,China

The effects of long-term fertilization on pools of soil organic carbon(SOC)have been well studied,but limited information is available on the oxidizable organic carbon(OOC)fractions,especially for the Loess Plateau in China.We evaluated the effects of a 15-year fertilization on the OOC fractions(F1,F2,F3 and F4)in the 0–20 and 20–40 cm soil layers in flat farmland under nine treatments(N(nitrogen,urea),P(phosphorus,monocalcium phosphate),M(organic fertilizer,composted sheep manure),N+P(NP),M+N(MN),M+P(MP),M+N+P(MNP),CK(control,no fertilizer)and bare land(BL,no crops or fertilizer)).SOC content increased more markedly in the treatment containing manure than in those with inorganic fertilizers alone.F1,F2,F4 and F3 accounted for 47%,27%,18% and 8% of total organic carbon,respectively.F1 was a more sensitive index than the other C fractions in the sensitivity index(SI)analysis.F1 and F2 were highly correlated with total nitrogen(TN)and available nitrogen(AN),F3 was negatively correlated with p H and F4 was correlated with TN.A cluster analysis showed that the treatments containing manure formed one group,and the other treatments formed another group,which indicated the different effects of fertilization on soil properties.Long-term fertilization with inorganic fertilizer increased the F4 fraction while manure fertilizer not only increased labile fractions(F1)in a short time,but also increased passive fraction(F4)over a longer term.The mixed fertilizer mainly affected F3 fraction.The study demonstrated that manure fertilizer was recommended to use in the farmland on the Loess Plateau for the long-term sustainability of agriculture.     

丁草胺对湿地芦苇生长发育及土壤酶活性的影响

在松嫩平原西部灌区盐碱芦苇湿地集中分布区,通过大田模拟试验,研究了除草剂丁草胺对湿地芦苇的生态响应特征及湿地土壤酶活性的影响。结果表明:添加到盐碱芦苇湿地的不同浓度(360、720和1 440μg/L)的丁草胺,1个月内均消减较快,丁草胺各浓度处理对湿地芦苇形态学指标(株高增长量、茎粗和干重)均未产生显著影响;在芦苇生理方面,随丁草胺质量浓度的增加,芦苇光合速率、气孔导度的降低趋势及胞间CO_2浓度和蒸腾速率的上升趋势在各处理间差异均未达到显著水平,但芦苇中可溶性糖含量显著降低。不同浓度丁草胺对湿地土壤酶活性的影响差异较大,在中、高浓度丁草胺作用下,土壤过氧化氢酶活性显著被抑制,碱性磷酸酶活性未受到显著影响;土壤蔗糖酶活性在中浓度丁草胺作用下有显著提高,其他浓度处理则无显著影响。结果显示,丁草胺在质量浓度≤1 440μg/L处理时,对盐碱湿地芦苇及土壤酶活性未表现出显著的毒理风险。     

气相色谱法测定敌敌畏在水稻中的残留

建立了气相色谱(GC-NPD)检测敌敌畏在水稻中残留量的方法。在优化样品前处理方法的基础上,选用DB-35(30m×0.32mm×0.25μm)毛细管色谱柱测定水稻植株、糙米、稻壳、土壤及田水样品中敌敌畏的含量。结果表明,在0.01~1.0mg/kg添加水平范围内,敌敌畏的平均添加回收率为82.2%~106.8%,相对标准偏差(RSD)为0.25%~3.67%,5%(n=5)。该方法操作简便,杂质干扰少,灵敏度、准确度高。     

Long-term effects of gravel―sand mulch on soil organic carbon and nitrogen in the Loess Plateau of northwestern China

Gravel–sand mulch has been used for centuries to conserve water in the Loess Plateau of northwestern China. In this study, we assessed the influence of long-term(1996–2012) gravel–sand mulching of cultivated soils on total organic carbon(TOC), light fraction organic carbon(LFOC), microbial biomass carbon(MBC), total organic nitrogen(TON), particulate organic carbon(POC), mineral-associated organic carbon(MOC), permanganate-oxidizable carbon(KMn O4-C), and non-KMn O4-C at 0–60 cm depths. Mulching durations were 7, 11 and 16 years, with a non-mulched control. Compared to the control, there was no significant and consistently positive effect of the mulch on TOC, POC, MOC, KMn O4-C and non-KMn O4-C before 11 years of mulching, and these organic C fractions generally decreased significantly by 16 years. LFOC, TON and MBC to at a 0–20 cm depth increased with increasing mulching duration until 11 years, and then these fractions decreased significantly between 11 and 16 years, reaching values comparable to or lower than those in the control. KMn O4-C was most strongly correlated with the labile soil C fractions. Our findings suggest that although gravel–sand mulch may conserve soil moisture, it may also lead to long-term decreases in labile soil organic C fractions and total organic N in the study area. The addition of manure or composted manure would be a good choice to reverse the soil deterioration that occurs after 11 years by increasing the inputs of organic matter.     

The potential for Fusarium oxysporum f. sp. fragariae,cause of fusarium wilt of strawberry,to colonize organic matter in soil and persist through anaerobic soil disinfestation

Fusarium wilt of strawberry, caused by Fusarium oxysporum f. sp. fragariae, is a disease of primary concern for strawberry production in many countries. Crop rotation and anaerobic soil disinfestation (ASD) have gained recent interest for their potential to contribute to management of this disease. Both techniques involve incorporation of organic matter into soil, which may be utilized by strains of Fusarium that are competitive saprophytes. We show that F. oxysporum f. sp. fragariae can colonize strawberry, lettuce, raspberry, and broccoli leaf tissues, which are sources of organic matter generated during crop rotation. This pathogen increased in soil population density during ASD treatments that did not become anaerobic, possibly as a result of growth on the organic amendment. However, significant population decreases were observed after ASD treatment when at least 100,000 cumulative reduced mV hours occurred in a 14-day experiment. Post-ASD abundance of F. oxysporum f. sp. fragariae in soil was negatively correlated with cumulative reduced mV hours. The only treatment that consistently caused disinfestation was exposed to a maximum temperature of 22 °C, which indicates there is potential for developing effective ASD treatments in the cool climates where strawberries are grown. Awareness that F. oxysporum f. sp. fragariae can act as a competitive soil saprophyte should be further investigated for its potential to alter disease outcomes where organic amendments are applied.     

Role of organic acids in the mechanisms of biological soil disinfestation (BSD)

Biological soil disinfestation (BSD), or reductive soil disinfestation, achieved by amendment with organic materials such as wheat bran followed by flooding and covering the soil surface, has been used to control some soilborne diseases including Fusarium wilt and bacterial wilt of tomato. During a BSD treatment, accumulation of acetic acid and/or butyric acid was detected with high-performance liquid chromatography. Survival of Fusarium oxysporum f. sp. lycopersici or Ralstonia solanacearum was suppressed by these organic acids. Amendment of these organic acids into soil suppressed the survival of R. solanacearum at lower concentrations than the maximum detected in BSD treatment, indicating that production of these organic acids is one of the mechanisms of control. However, F. oxysporum f. sp. lycopersici in soil survived with the maximum concentrations of these organic acids achieved by BSD; thus, involvement of factors other than organic acids may be involved.     

Biological soil disinfestation using ethanol: effect on <Emphasis Type="Italic">Fusarium oxysporum</Emphasis> f. sp. <Emphasis Type="Italic">lycopersici</Emphasis> and soil microorganisms

Better soil disinfestation methods, such as biological soil disinfestation (BSD), that are environmentally safe are increasingly been developed and used because of rising concerns related to environmental risks. We evaluated the efficacy of soil disinfestation using ethanol to control the fungus Fusarium oxysporum f. sp. lycopersici, which causes fusarium wilt of tomato. Survival of bud cells and chlamydospores declined markedly in soil saturated with diluted ethanol solution in the laboratory. In field trials, artificially added nonpathogenic Fusarium oxysporum and indigenous F. oxysporum were both strongly suppressed in soil saturated with 1% ethanol solution; a wheat bran treatment was not as effective. The artificially added fungus was not detected in three of four sites treated with ethanol but was detected in three of four sites amended with wheat bran. Using ethanol in pre-autoclaved soil was not suppressive; thus native microorganisms are essential for the suppression. This ethanol-mediated biological soil disinfestation (Et-BSD) temporarily increased the number of anaerobic bacteria, but the number of fungi and aerobic bacteria was stable. Polymerase chain reaction–denaturing gradient gel electrophoresis (PCR–DGGE) analysis revealed slight but apparent differences in bacterial community structures in the soil treated with Et-BSD compared with the structure in soils after other treatments such as water irrigation and in the control soil, which received neither organic amendment nor irrigation after 15 days. Et-BSD is a potentially effective and easy soil disinfestation method, and its impact on native, beneficial microorganisms is moderate.     

Application to soil of disinfectants through irrigation reduces Verticillium dahliae in the soil and verticillium wilt of olive

The application of disinfectants through drip irrigation could be a feasible practice against verticillium wilt (Verticillium dahliae) of olive. OX-VIRIN (activated peroxide) and OX-AGUA AL25 (quaternary ammonium compounds) are two disinfectants that have shown efficacy against V. dahliae in irrigation water and potential for reducing the disease in young olive plants. In this work, various post-planting application strategies incorporating OX-VIRIN (once a month, or twice a month on alternate or successive weeks) or OX-AGUA AL25 (once a month, or twice a month on alternate weeks) were assessed for their effect on V. dahliae in soil, disease in olive trees, and olive yield, in a 2-year pot-experiment under natural environmental conditions. The disinfectants were injected via metering pumps into a drip irrigation system that irrigated olive trees planted in V. dahliae-inoculated soil. All the application strategies significantly reduced the total inoculum density in soil compared to controls with no disinfectants and noninoculated soil. The microsclerotia density was also significantly reduced in disinfested soils by 73.6–86.8%, depending on the strategy. The symptoms and infection incidence were always lower in treatments subjected to disinfestation. The treatment with OX-AGUA AL25 applied twice a month on alternate weeks most reduced the symptoms (by 53.0%) and colonization index (by 70.8%) with respect to untreated water control. This soil disinfestation also significantly strengthened the symptom remission. Tree growth and production were negatively affected by soil inoculation (reduced by 45.6% and 88.7%, respectively), but not so by disinfectants, which even relieved the reduction in inoculated soils, especially when OX-AGUA AL25 was applied.     

不同覆盖方式对旱作农田土壤碳氮含量及玉米产量的影响

设置垄膜沟播(R)、平作全覆膜(P)和平作秸秆覆盖(S)3种不同覆盖栽培模式,以传统平作(CK)为对照,通过3 a大田定位试验,研究不同覆盖方式对农田土壤碳氮及其组分变化、玉米干物质积累和籽粒产量的影响。结果表明:连续覆盖3 a后,R和P处理土壤有机碳和全氮含量及储量均呈逐渐下降趋势,而S处理则呈上升趋势且每年的增幅逐渐增大;各处理0～40 cm土层土壤有机碳储量均表现为S>R>P>CK,全氮储量表现为S>CK>R>P,S处理有机碳和全氮储量分别较CK提高3.4%和7.5%;与CK相比,R和P处理可提高土壤碳氮比,在0～20 cm土层平均分别提高7.9%和9.6%(P<0.05),而S处理0～20 cm土层土壤碳氮比平均降低了9.9%(P<0.05);各覆膜处理(R和P)均较CK显著降低了0～40 cm土层土壤可溶性有机碳(DOC)和土壤可溶性有机氮(DON)含量,而S处理较CK处理0～60 cm土层DOC和DON含量2 a平均均提高6.2%;各处理收获期硝态氮含量在0～100 cm剖面中的垂直分布与CK无明显差异;各处理土壤铵态氮含量均...     

黄河口翅碱蓬湿地土壤氮的季节变化

2009年4～11月,选择位于黄河口滨岸中潮滩和低潮滩上的翅碱蓬湿地为研究对象,对比研究了湿地土壤氮的季节变化特征。结果表明:两种湿地不同土层的NO3--N、NH4+-N和TN含量均具有明显的季节变化特征,但不同类型湿地之间差异较大。二者NO3--N、NH4+-N含量的季节差异主要与其所受潮汐影响程度及其引起的无机氮物理运移和对NH4+-N吸附能力的差异有关。二者在7月较低的NO3--N含量主要与植物对土壤中有效氮的大量吸收与利用有关,而8月较高的NO3--N、NH4+-N含量主要与此间土壤有机氮矿化较强有关。两种湿地不同土层的TN含量与C/N的季节变化规律整体相反。二者TN含量的差异主要受制于不同土层的有机质分布以及潮汐的影响状况,而C/N的差异主要与两种湿地在不同时期受陆源的影响程度有关。     

我国土壤熏蒸消毒60年回顾

熏蒸剂棉隆和氯化苦于20世纪60年代在我国曾用于棉花黄萎病的防治,由于缺乏施药机械、相关施药标准和方法,加之与国外交流甚少,虽然发现熏蒸剂对土传病害有一定的效果,但一直未形成规模化应用。我国大量使用熏蒸剂进行土壤消毒处理始于20世纪90年代,随着溴甲烷的引进与广泛应用,溴甲烷的替代技术及产品研发,开启了我国土壤熏蒸消毒的新纪元。伴随着土壤消毒技术与理论的不断丰富,新型配套机械装备的成功研发,社会化服务模式的广泛运用,土壤熏蒸消毒在我国进入快速发展时期,大批专业化服务组织应运而生,为我国农作物病虫害的绿色防控、实施乡村振兴战略提供了强有力的技术支撑。该文从土壤熏蒸剂的种类、发展历史、基础理论的建立与阐明3方面综述了我国土壤熏蒸发展情况,并对我国土壤熏蒸消毒未来发展方向提出了展望。     

土壤厌氧消毒技术、作用机理及其防治效果的影响因素

土壤厌氧消毒(anaerobic soil disinfestation,ASD)技术作为一种环境友好的非化学土壤消毒技术能有效防治真菌、细菌、线虫和杂草等引起的土传病害。该技术已在多个国家广泛应用并被证实可显著增加作物产量。ASD技术基本流程为向土壤中添加适量有机碳源、补充足够水分和用塑料薄膜覆膜密闭2～15周。ASD技术对土传病害防控的作用机理为有机碳源分解产生的挥发性有机物对土传病原菌有抑制作用,土壤理化指标的改变(如pH降低、有机质增加等)能提升土壤质量,从而提高作物抗逆能力,土壤厌氧条件促进土壤微生物群落结构及多样性的改变,通过生物竞争和结构重建改善土壤微生态环境,降低土传病害发生风险。ASD技术对土传病害的防治效果也因有机碳源类别、土壤理化性质及覆盖薄膜的不同而存在差异。因此,优化ASD技术试验条件、深入探索ASD技术作用机理、挖掘更多有灭菌活性的挥发性物质或有益生防菌是未来ASD技术的研究重点。同时,ASD技术与其他土壤消毒技术结合使用,有助于提高其防治效果,扩大应用范围。