Comparison of weigh-suckle-weigh and machine milking for measuring ewe milk production.

Thirteen crossbred ewes were used to compare weigh-suckle-weigh (WSW) and machine milking (MM) methods for determining milk production of ewes that were rearing single or twin lambs. At parturition, ewes were 13 mo of age and produced six single lambs and seven pairs of twin lambs. Milk production estimates were initiated on d 6 of lactation and a 3-d rotation of the two techniques was implemented. On d 6, milk production was measured using WSW; on d 7, MM was used. No measurement was made on d 8. The 3-d rotation was repeated 20 times throughout a 63-d lactation, resulting in 20 point estimates of milk production for each method of measurement for each ewe. The WSW procedure consisted of a 3-h period in which lambs were withheld from suckling their dams. This was followed by a suckling period, a second 3-h withholding period, and a second suckling period. Differences in pre- and postsuckling lamb weights of the second suckling period were defined as milk consumption and, indirectly, 3-h milk production. The MM procedure included an administration of 10 IU of oxytocin (i.v.), followed by evacuation of the udder with a machine using commercially available sheep milking equipment, and the milk was discarded. Lambs were withheld from suckling the ewes for a 3-h period, followed by a repetition of the oxytocin and machine milking procedures. Milk from the second milking was weighed. Milk production estimates determined using the WSW and MM techniques were similar (P = .42). Average 3-h milk production was 340 and 351 g for WSW and MM, respectively. Machine milking provides a reliable tool in evaluating the milk-producing ability of ewes that are rearing single or twin lambs.     

挤奶频率对奶牛生产性能的影响

文章介绍了挤奶频率对奶牛泌乳性能、繁殖性能、健康与福利等方面的影响。     

Review of milk let-down in camels and proposition of a milk recording method

Tropical Animal Health and Production - The aim of this article was to review the milk let-down in camels and to propose a simple milk recording method. Milking of camels generally starts between 1...     

Efficacy of ivermectin against gastrointestinal nematodes in dromedary camels

The anthelmintic activity of ivermectin when administered orally and subcutaneously at a dosage of 0.2 mg/kg of body weight was evaluated in 4 trials involving 20 dromedary camels (Camelus dromedarius). Fecal egg counts were made on the day of treatment and at 2, 4, and 10 weeks after treatment. Trichostrongylid egg counts were reduced by a maximum of 100% when ivermectin was administered orally and by greater than 88% when given subcutaneously. Egg counts of Trichuris spp were reduced by greater than 85% with oral administration, but increased following subcutaneous treatment. No adverse local or systemic reactions were observed following treatment with ivermectin by either route.     

Aetiological analysis and diagnosis of reproductive disorders in male dromedary camels

The purpose of this paper was to analyse the aetiology and methods of diagnosing reproductive disorders in male dromedary camels. Male camel infertility manifests as one of three conditions: post-coital infertility (IG), inability to copulate (IC) and lack of sexual desire (LSD). IG is mainly a testicular disorder that is linked to a deteriorated seminogram, arrested spermatogenesis, Sertoli cell-only syndrome and testicular degeneration. For IG diagnosis, semen analysis, testicular biopsy and fine-needle aspiration are gold standards. Testicular ultrasonography was generally inefficient. High serum FSH was found in IG camels with oligo- and azoospermia, implying primary spermatogenesis defects. The testis-expressed protein (TEX101) and the epididymis-expressed protein (ECM1) are reliable biomarkers for distinguishing between obstructive and non-obstructive azoospermia. IC manifests in two forms: phimosis (PHI) and erectile dysfunction (ED). PHI is frequently linked to preputial and penile pathologies, as well as leucocytosis, neutrophilia and elevated nitric oxide metabolites. The majority of camels with ED have normal genital organs, and the condition is associated with an increase in cardiac troponin I. LSD is a rare disorder brought on by hormonal imbalances, high temperatures, stress and debilitating diseases. In conclusion, IG diagnosis necessitates semen analysis, testicular biopsy or fine-needle aspiration, and FSH testing, whereas IC diagnosis requires preputial and penile examinations. Diagnostic aids include serum and seminal biomarkers.     

Pharmacokinetics of progesterone in dromedary camels (Camelus dromedarim)

The effect of ovariectomy or administration of progesterone (P4) on the disposition kinetics of P4 was determined in dromedary camels. The disappearance of P4 from peripheral circulation of the camel following ovariectomy or after a single intravenous (I.V.) injection of 1 mg kg(-1) body weight followed a bioexponential curve. Both curves were parallel indicating that the disappearance of injected P4 behaved similarly to endogenous P4. The mean (+/- SD) half-life calculated from the slower component of this decline, was 26.0 +/- 2.0 min after I.V. injection of P4 and 28.0 +/- 2.1 min after ovariectomy. The apparent volume of distribution (1370 ml kg(-1)) exceeded total body water suggesting extensive tissue penetration.     

Activity of some enzymes in the serum of dromedary camels.

The activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), acid phosphatase (ACP), lactate dehydrogenase (LDH) and isocitric dehydrogenase (ICD) in the serum of 60 healthy dromedary camels of either sex and different ages (one to 25 years) were determined. The results were analysed with respect to time of year (December-January and May-June), sex and age groups (below four years; four to 10 years; and over 10 years). The overall mean activities of AST, ALT, ALP, ACP, LDH and ICD were 36.1 +/- 0.35, 4.65 +/- 0.35, 27.21 +/- 0.43, 7.18 +/- 0.21, 479.0 +/- 7.33 and 7.74 +/- 0.17 iu litre-1, respectively. Activities of AST, ALT, ALP and ACP were significantly higher during extremely hot conditions (May-June) than in extreme cold (December-January) while the activity of LDH was higher in extremely cold conditions. Analysis of data based on sex revealed that AST, ALT and ALP activities in the serum of male animals were significantly higher than in female animals. The activities of all the enzymes were highest in animals under four years and then gradually decreased with age being lowest in the animals over 10 years.     

Technical note: machine milking of sows--lactational milk yield and litter weights.

This study was designed to 1) determine milk yield of sows that were machine milked; 2) assess the effects of pulsation rate, pulsation ratio, and pig removal on milk yield; and 3) assess litter weights. In Exp. 1, four sows were milked daily to 60 d postpartum. There were differences (P < .05) in milk yield among sows, the greatest being 1,898 mL/d. Daily milk yield peaked between 15 and 25 d postpartum. Litter weights were 18.0 +/- 1.0 kg at farrowing and 60.8 +/- 12.1 kg at d 60. In Exp. 2, four sows were milked daily for 28 d. Pulsation rate and ratio (150/min and 28:72, milk:rest, and 60/min and 50:50) were alternated on a daily basis and pigs were isolated for either 0 or 60 min before milking. The higher pulsation rate resulted in more milk per milking (202 +/- 13 vs 168 +/- 13 mL; P < .05). Pig removal resulted in 221 +/- 11 vs 148 +/- 14 mL milk (P < .01). Pig removal times and pulsation characteristics affect the amount of milk obtained, but milk removal from sows has a severe effect on litter weight. This system can be used to harvest sow's milk for pharmaceutical purposes, but supplementation of the pigs is necessary.     

Circadian rhythm of bone formation biomarkers in serum of dromedary camels

The circadian rhythm of biomarkers of bone formation including osteocalcin and bone alkaline phosphatase (BAP) was studied in the serum of dromedary camels. Blood samples were collected every 60 min for 24 h from 10 healthy adult female camels. ELISA was used to determine the concentrations of serum osteocalcin and BAP. The results showed a marked fluctuation in the concentration of osteocalcin during the 24 h period with minimum and maximum levels at 13:00 (01:00 pm) and 18:00 (06:00 pm), respectively. Slight fluctuation was observed in the concentration of BAP with minimum and maximum levels at 01:00 am and 12:00 pm, respectively. The correlation between the two biomarkers was weak. It was concluded that it is important to fix the time of blood sampling for analysis of osteocalcin concentrations, but not for BAP.     

Pharmacokinetics and intramuscular bioavailability of difloxacin in dromedary camels (Camelus dromedarius)

Single-dose disposition kinetics of difloxacin (5mg/kg bodyweight) were determined in clinically normal male dromedary camels (n=6) following intravenous (IV) and intramuscular (IM) administration. Difloxacin concentrations were determined by high performance liquid chromatography with fluorescence detection. The concentration-time data were analysed by compartmental and non-compartmental kinetic methods. Following a single IV injection, the plasma difloxacin concentration-time curve was best described by a two-compartment open model, with a distribution half-life (t(1/2alpha)) of 0.22+/-0.02h and an elimination half-life (t(1/2beta)) of 2.97+/-0.31h. Steady-state volume of distribution (V(dss)) and total body clearance (Cl(tot)) were 1.02+/-0.21L/kg and 0.24+/-0.07L/kg/h, respectively. Following IM administration, the absorption half-life (t(1)(/)(2ab)) and the mean absorption time (MAT) were 0.44+/-0.03h and 1.53+/-0.22h, respectively. The peak plasma concentration (C(max)) of 2.84+/-0.34microg/mL was achieved at 1.42+/-0.21h. The elimination half-life (t(1/2el)) and the mean residence time (MRT) was 3.46+/-0.42h and 5.61+/-0.23h, respectively. The in vitro plasma protein binding of difloxacin ranged from 28-43% and the absolute bioavailability following IM administration was 93.51+/-11.63%. Difloxacin could be useful for the treatment of bacterial infections in camels that are sensitive to this drug.     

Manipulating milk production in early lactation through photoperiod changes and milking frequency.

Photoperiod management and increased milking frequency in early lactation offer noninvasive methods to improve production and health in dairy cows. Prolactin physiology is critical to the responses, and thus may be a factor in other environmental effects (eg, temperature and stress). Integration of these strategies into the management system is not difficult, but requires attention to details of feeding, housing, and animal movement. The techniques can be combined effectively with other management strategies that increase milk yield. There is evidence that cows managed using photoperiod manipulation and frequent milking produce more milk than control animals, but also have improved transitions into lactation.     

Ovary and ovarian bursa in dromedary camels: Clinical relevance of the topographical features

This study aimed to associate ovarian characteristics with the efficiency of clinical examination and occurrence of genital diseases in dromedary camels. The reproductive tract of 870 female camels was examined through standard transrectal palpation and by ultrasonography during the breeding season. The ovaries were examined for structures and dimensions. The follicles were categorized according to size, the thickness of the wall and contents. Follicle aspiration was carried out from females with overgrown follicles (OVGF, n = 127), and the obtained follicular fluids were examined. At the slaughterhouse, 100 genital tracts were examined in situ and after dissection. Ovarian bursae were examined for patency and the presence of fluid (ovarian hydrobursitis, OVHB). Risks associated with the development of OVGF and OVHB were identified by the logistic regression. The results showed that, due to topographical difference, the right ovary was more accessible at rectal palpation than the left ovary (98.9% vs. 96.1%, p = .0005). Time needed for rectal palpation of the right ovary was shorter than the left ovary (25.1 ± 25 s vs. 34.6 ± 34.5 s, p = .03). Significant relationships were found between OVGF and OVHB (Odds ratio = 10.5, p = .001), OVGF and clinical endometritis (Odds ratio = 21.1, p = .001), OVGF and vaginal adhesion (Odds ratio = 4.4, p = .03), and OVHB and clinical endometritis (Odds ratio = 11.3, p = .001). Ultrasonographic examination was imperative for the differentiation between active corpus luteum, old non-active corpus luteum and small luteinized follicle. In conclusion, anatomical arrangement of the ovary and ovarian bursa in dromedary camels affects the likelihood of their accessibility during clinical examination and predisposes to unusual genital disorders.     

活酶宝对泌乳奶牛产奶量及乳成分的影响

本试验选用60头中国荷斯坦奶牛,按照胎次、体况及产奶量随机分成对照组和试验组。对照组饲喂原有全混合日粮(TMR);试验组在此基础上,每天每头牛在精料中添加17g的活酶宝。结果表明,活酶宝可以提高奶牛的产奶量、乳脂肪率、乳蛋白率、乳糖率、干物质率和脂蛋比(P>0.05),并降低体细胞数(P>0.05)。与对照组相比较,试验组泌乳量、乳脂肪率、乳蛋白率、乳糖率、干物质率的值分别相对提高1.7kg、0.23、0.12、0.09和0.27;体细胞数相对下降26.16百万个。     

Molecular detection of brucellosis in dromedary camels of Qatar by real-time PCR technique

Background and methodologyBrucellosis in livestock causes huge economic loses of developing countries and demonstrates a serious health risks to the patrons of infected dairy goods and meat. Prevention of brucellosis in cattle relies on the trustworthiness of the techniques utilized to detect the causative pathogen. In the current investigation, we described the exploration of the well-known real-time PCR technique based on the Brucella-specific IS711 with different tissue samples of dromedary camels in Qatar.ResultsThe findings of the real-time PCR unveiled the occurrence of Brucella spp in 60 % of lung tissues, 71.42 % of liver tissues, 72 % of spleen tissues, 25 % of kidney tissues, and 42.42 % of placental fluid samples. Among them, the liver tissues and spleen tissues possessed the highest number of positive results, whereas kidney tissues displayed the lowest number of positive results for Brucella spp.ConclusionThe findings of this investigation discloses that PCR technique is a sensitive and specific technique for the identification of Brucella spp. in dromedary camels. Most of the tissues samples showed the presence of Brucella spp. and also we concluded that there is a need for further studies to be conducted in order to identify specific species of Brucella.     

Tuberculosis in dromedary camels slaughtered in Nigeria: a documentation of lesions at postmortem

Tropical Animal Health and Production - In comparison with other livestock, tuberculosis (TB) in camels has not been extensively studied in Nigeria. Camels in the hands of Nigerian pastoralists...     

Tuberculosis outbreak in a dromedary racing herd and rapid serological detection of infected camels

A recent outbreak of tuberculosis (TB) in a dromedary racing herd of 58 animals involved 3 infected animals. Disease was confirmed at necropsy by finding gross lesions from which Mycobacterium bovis (antelope type) was isolated. Sera collected from the camels in this herd were used to evaluate two new serological methods, Multiantigen Print Immunoassay (MAPIA) and rapid test (RT) developed using the lateral-flow technology, in comparison with the intradermal tuberculin tests. Antibodies were found in all three infected dromedaries by both RT and MAPIA, but not in the remaining 55 animals in the herd. With the limited number of animals tested in this study, the serological assays showed the potential for convenient, rapid, and accurate diagnosis of TB in live camels.