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养分胁迫对籽粒灌浆期水稻叶片衰老影响的蛋白质组学分析 总被引:1,自引:0,他引:1
采用蛋白质组学分析技术,对养分胁迫下杂交水稻威优916功能叶片蛋白质组差异表达进行研究,以揭示养分胁迫影响籽粒灌浆期水稻衰老的分子机理。经双向电泳分离,从功能叶中获得34个差异表达蛋白质,其中26个蛋白质功能得到鉴定,包括11个参与光合作用蛋白质,4个参与呼吸作用蛋白质,10个参与抗氧化及信号传导蛋白质和1个未知功能蛋白质。分析结果表明,养分胁迫下,灌浆期水稻叶片光合作用及呼吸作用显著下降;清除活性氧类蛋白质表达量下调,活性氧生成蛋白质及胁迫信号传导蛋白质表达量上调,水稻抗性减弱,叶片中活性氧积累,水稻衰老加剧。 相似文献
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为了从蛋白质水平进一步揭示小麦抗旱机理,以旱地小麦品种晋麦47为试验材料,采用蛋白质双向电泳技术,结合质谱鉴定技术,研究幼苗在正常供水与水分胁迫(-0.5 MPa的PEG-6000溶液处理48h)后叶片蛋白质组表达谱间的差异,分析水分胁迫差异表达的蛋白种类及其功能.研究发现,在正常供水和水分胁迫处理两种条件下,幼苗叶片蛋白的双向电泳图谱均可重复检测到850个蛋白点;31个蛋白点在两种供水水平间表达量存在显著差异,其中17个蛋白点在水分胁迫处理下表达量明显增加,14个蛋白点表达量明显减少.对这些蛋白点进行肽指纹图谱分析,并利用Mascot软件在NCBInr数据库搜索,最终有26个蛋白点被成功鉴定;其中,未知功能蛋白7个;已知功能蛋白19个,主要涉及能量、代谢、抗病与防御、细胞结构、转录与翻译以及信号转导等功能类别.结果表明,小麦体内的多个代谢及调控途径参与了对干旱的应激反应.最后,本文还对鉴定到的差异蛋白点与水分胁迫的关系进行了分析和讨论. 相似文献
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对大豆光周期敏感型品种东农42 分别进行16 h光照和8 h光照处理,提取处理和对照相同叶位叶片的全蛋白质进行双向电泳,通过PDQuest和液相离子阱质谱(LC-ESI-MS)技术对差异表达蛋白进行分析,获得二级质谱图谱,用Mascot软件搜索NCBInr数据库,鉴定出16个差异表达蛋白,其中12个表达量上调,4个表达量下调.对谷胱甘肽S-转移酶、二硫键异构酶、TCTP、半胱氨酸合成酶、Rieske铁硫蛋白前体、金属蛋白酶等主要差异蛋白进行功能分析,表明这些蛋白与植物的光合作用、信号转导、细胞凋亡的调控、防卫解毒和能量代谢等多种植物的生理生化功能相关. 相似文献
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从蛋白质组学角度分析了在养分胁迫下用6-BA培养的水稻的叶片蛋白质组变化,以揭示6-BA维持养分胁迫下水稻生长的分子机理。分别提取用6-BA和蒸馏水培养的水稻叶片的蛋白质,经双向电泳进行分离,应用Image Master^TM 2D platinum 5.0软件对所得到的双向电泳图谱进行分析比较,共得到24个明显的差异表达蛋白质点。蛋白质是细胞功能的执行者,受细胞功能或细胞环境变化的影响,因此,上述蛋白质发生变化应与在养分胁迫下6-BA维持水稻叶片生长的作用有关。 相似文献
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为从蛋白组学角度认识小麦抗白粉病机制,以含有抗白粉病新基因Pm40的小麦品系L699为材料,采用蛋白质双向电泳和质谱技术(MALDI-TOF-MS)检测小麦叶片接种白粉菌24h后的差异蛋白。结果表明,经PDQuest软件分析,接种白粉菌后小麦叶片中有18个蛋白质斑点表达量上调,38个蛋白质斑点表达量下调。对表达量上调的蛋白质斑点进行质谱分析和数据库检索,共鉴定出15种蛋白质,其中13种参与防御反应、碳水化合物代谢和蛋白质周转。这些差异蛋白与抗病途径、植株生理过程密切相关,在小麦抗白粉病过程中起很重要的作用。 相似文献
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大豆是我国重要的农作物之一,蛋白质组学技术是解析植物响应逆境胁迫的重要工具之一。为进一步推进对响应干旱胁迫的大豆叶片蛋白的鉴定工作,以大豆幼苗的叶片为研究对象,用甘露醇溶液模拟干旱胁迫,处理24 h后提取叶片蛋白粉进行双向电泳分离和图谱差异分析。结果表明:采取三氯醋酸(TCA)-丙酮沉淀法提取的叶片蛋白进行双向电泳分离能够得到稳定清晰的双向电泳蛋白图谱。采取PD-Quest软件对干旱胁迫下的大豆叶片蛋白表达图谱进行差异分析,共检测和匹配425个大豆叶片蛋白点,其中差异表达的蛋白点有101个,60个表达量上调,41个表达量下调。本研究结果表明有一部分大豆叶片蛋白响应了干旱胁迫。 相似文献
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以杂交早稻威优916为试验材料,采用水培方式栽培,生育后期设置养分胁迫和全营养液两种处理,从蛋白质组学角度研究后期持续的养分胁迫对水稻籽粒灌浆的影响,以期为水稻高产栽培提供科学依据。籽粒蛋白质经双向电泳分离后共获得了37个发生差异表达的蛋白质,经串联质谱分析(ESI Q MS/MS),27个蛋白质功能得到鉴定,包括4个参与光合作用的蛋白质、13个与籽粒充实发育相关的蛋白质、9个逆境相关的蛋白质及1个呼吸代谢相关的蛋白质。分析结果表明,养分胁迫下,逆境相关蛋白质在籽粒灌浆的前中期大量上调表达,籽粒光合作用减弱,后期呼吸作用增强,籽粒的氮代谢受到显著影响,谷蛋白及胚蛋白表达相对滞后,蛋白质和脂肪合成减少,从而造成籽粒灌浆不足,产量降低。 相似文献
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GE Cai-lin WANG Ze-gang WAN Ding-zhen DING Yan WANG Yu-long SHANG Qi LUO Shi-shi 《水稻科学》2009,16(1):33-44
A proteomic approach including two-dimensional electrophoresis and mass spectrometric (MALDI-TOF MS) analyses was used to investigate the responses to cadmium (Cd) stress in seedlings of rice (Oryza sativa L.) varieties Shanyou 63 and Aizaizhan. Cd stress significantly inhibited root and shoot growth, and affected the global proteome in rice roots and leaves, which induced or upregulated the expression of corresponding proteins in rice roots and leaves when rice seedlings were exposed to 0.1 or 1.0 mmol/L Cd. The Cd-induced proteins are involved in chelation and compartmentation of Cd, elimination of active oxygen free radicals, detoxification of toxic substances, degradation of denatured proteins or inactivated enzymes, regulation of physiologic metabolism and induction of pathogenesis-related proteins. Comparing the Cd-induced proteins between the two varieties, the β-glucosidase and pathogenesis-related protein family 10 proteins were more drastically induced by Cd stress in roots and leaves of Aizaizhan, and the UDP-glucose protein transglucosylase and translational elongation factor Tu were induced by 0.1 mmol/L Cd stress in roots of Shanyou 63. This may be one of the important mechanisms for higher tolerance to Cd stress in Shanyou 63 than in Aizaizhan. 相似文献
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以中旱3号、汕优63和矮仔占为材料,在10% PEG6000模拟干旱条件下,分别应用基因芯片和mRNA 差异显示技术检测了不同基因型水稻叶片中活性甲基循环、转移相关基因表达对干旱胁迫的应答。中旱3号和汕优63叶片中活性甲基循环过程受干旱胁迫诱导,而矮仔占叶片中这一过程受到干旱胁迫的抑制;干旱胁迫诱导中旱3号叶片中更多甲基转移酶基因的表达,尤其是对Rubisco蛋白甲基化修饰基因转录的诱导,有利于防止Rubisco蛋白的氧化降解;干旱胁迫抑制矮仔占叶片中DNA甲基转移酶、组蛋白甲基转移酶基因的转录。这一结果证明水稻中甲基循环、转移基因的表达在水稻耐旱中有一定的作用。 相似文献
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A leaf inclination angle distribution model, which is applicable to simulate leaf inclination angle distribution in six heights of layered canopy at different growth stages, was established by component factors affecting plant type in rice. The accuracy of the simulation results was validated by measured values from a field experiment. The coefficient of determination (R2) and the root mean square error (RMSE) between the simulated and measured values were 0.9472 and 3.93%, respectively. The simulation results showed that the distribution of leaf inclination angles differed among the three plant types. The leaf inclination angles were larger in the compact variety Liangyoupeijiu with erect leaves than in the loose variety Shanyou 63 with droopy leaves and the intermediate variety Liangyou Y06. The leaf inclination angles were distributed in the lower range in Shanyou 63, which matched up with field measurements. The distribution of leaf inclination angles in the same variety changed throughout the seven growth stages. The leaf inclination angles enlarged gradually from transplanting to booting. During the post-booting period, the leaf inclination angle increased in Shanyou 63 and Liangyou Y06, but changed little in Liangyoupeijiu. At every growth stage of each variety, canopy leaf inclination angle distribution on the six heights of canopy layers was variable. As canopy height increased, the layered leaf area index (LAI) decreased in all the three plant types. However, while the leaf inclination angles showed little change in Liangyoupeijiu, they became larger in Shanyou 63 but smaller in Liangyou Y06. The simulation results used in the constructed model were very similar to the actual measurement values. The model provides a method for estimating canopy leaf inclination angle distribution in rice production. 相似文献
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三个C两优杂交稻组合的株型及干物质生产特性 总被引:2,自引:0,他引:2
以汕优63为对照,对3个C两优杂交稻组合的株型及干物质生产特性进行了研究。与汕优63相比,C两优杂交稻组合上3叶叶型较狭长,叶片着生角度小,总叶面积较大;齐穗期上3叶净光合速率和叶绿素含量较高,根系活力较强;基部节间较粗、较短,茎秆的抗倒性强, 中部节间较长,穗下节间占总秆长的42.77%;抽穗后干物质积累速率快,增量大,且茎鞘叶中的干物质向籽粒运转量多,对籽粒的贡献率高。C两优杂交稻抽穗后茎叶干物质输出量和干物质积累量与稻谷产量分别呈显著和极显著正相关。C两优杂交稻组合较理想的株型和中后期强大的干物质生产和转运优势是其高产的重要原因。 相似文献
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两系杂交稻组合两优培九和65396的光合形态特征研究 总被引:13,自引:0,他引:13
采用分期播种和平行比较方法,对两系杂交稻组合两优培九和 65396 的光合形态性状进行了研究。与汕优 63 相比,两优培九和 65396 在单株绿叶数达到最大值之前的增长速率基本一致,但其后的衰减速率却明显要慢,且功能叶较厚、叶角小、叶凹度大,光合优势明显。若以叶面积×叶重系数为综合光合特性,则 3 个组合中,65396 功能叶的光合优势最佳。 相似文献
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水稻育秧肥的壮秧效应及其蛋白质组学分析 总被引:3,自引:0,他引:3
通过比较育秧肥和化肥作基肥的早稻秧苗质量的差异,及在这两种育秧条件下水稻叶片的蛋白质组表达差异,揭示了育秧肥的壮秧机理。结果表明,育秧肥具有促进秧苗分蘖和发根,使秧苗矮壮,提高秧苗叶绿素含量和光合生产能力,提高秧苗综合素质等作用。经双向电泳分离,获得了18个不同处理下发生差异表达的蛋白质。经质谱分析,其中16个蛋白质得到鉴定,包括9个参与光合作用的蛋白质,3个与蛋白质合成密切相关的蛋白质以及3个与抗性相关的蛋白质和1个未知功能蛋白质。 相似文献
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TANG Wen-bang ZHANG Gui-lian XIAO Ying-hui DENG Hua-bing FAN Ke LIU Guo-hua CHEN Li-yun 《水稻科学》2010,17(4):319-325
The contents of chlorophyll,soluble sugars,soluble proteins and thiobarbituric acid reaction substance(TBARS),chlorophyll fluorescence parameters,net photosynthetic rate as well as the activities of superoxide dismutase(SOD)and peroxidase(POD)of flag leaves at the late growth stages were studied by using C Liangyou series of hybrid rice combinitions as material and Shanyou 63 as control.The C Liangyou series of hybrid rice combinations used in the experiment included C Liangyou 396,C Liangyou 87,C Liangyou 755 and C Liangyou 34,which all used C815S as male sterile line.The contents of chlorophyll,soluble sugars and soluble proteins in flag leaves of the C Liangyou series combinations at the late growth stages were higher than those of the control,whereas the TBARS content was lower than that of the control.The activities of SOD and POD were significantly higher than those of the control on the 7th day after heading,and then decreased slowly.ΦPSIIvalue and qP value of flag leaves decreased at the late growth stages,and these two parameters in flag leaves of the C Liangyou series combinations were higher than those of the control,while the qN value increased at the late growth stages and was lower than that of the control.The net photosynthetic rate of flag leaves at the late growth stage was higher compared with the control.These results suggest that slow senescence and strong photosynthetic capability in flag leaves at the late growth stages are the physiological basis of the C Liangyou series combinations. 相似文献
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超级杂交稻两优培九剑叶叶鞘叶绿体光化学活性的初步研究 总被引:4,自引:0,他引:4
以超级杂交稻两优培九及其父本9311、母本培矮64S和三系杂交稻组合汕优63为研究材料,采用光合膜人工反应体系和32Pi同位素标记等技术,初步研究了超级杂交稻两优培九剑叶叶鞘的光化学活性。结果表明,两优培九剑叶叶鞘及其叶片光系统Ⅰ还原能力的均值分别比汕优63高6.35和15.31 μmol/(mg·h),光系统Ⅱ放氧活性的均值分别高出616和9.89 μmol/(mg·h),且大多数时期达显著或极显著水平;两优培九剑叶叶鞘及其叶片浸出液钙含量均显著或极显著高于汕优63;两优培九剑叶叶鞘及其叶片叶绿体膜上Mg2+ ATPase活性的均值均高于汕优63,大多数时期达显著或极显著水平;两优培九剑叶叶鞘及其叶片循环光合磷酸化活性的均值也均高于汕优63,近一半时期达显著或极显著水平;非循环光合磷酸化活性的均值分别比汕优63高0.25和0.31 μmol/(mg·h),也近一半时期达显著或极显著水平;两优培九剑叶叶鞘及其叶片的光化学活性大多数时期显著或极显著高于亲本,具有平均优势或超亲优势;与叶片相比,剑叶叶鞘完全具有同剑叶一样的光化学活性,两者没有质的区别;两优培九剑叶叶鞘具有较高的光化学活性可能是它具有超高产特性的重要原因之一。 相似文献