扁穗牛鞭草新品种选育及栽培技术

1997～1999年在四川雅安青农江江滩采集野生扁穗牛鞭草,通过无性系选择选出新品系H2003-5;2003～2006年,在雅安市四川农业大学草学系基地进行品比试验;2006～2008年,在四川雅安、洪雅、南充和重庆荣昌参加区域试验和生产试验;2009年经全国草品种审定委员会审定通过,作为野生栽培驯化品种登记,命名为“雅安”扁穗牛鞭草,新品种产草量高,适应性强,是优良的多年生暖季型牧草,适合在长江中下游地区种植.     

Behaviour of domestic ducks exposed to mobile predator stimuli. 2. The association of individual duckling attributes with relative position in a flock

1. We investigated whether an individual duckling's (Anas platyrhynchos domesticus) attributes were associated with its response to an approaching human when it was a member of one of three flocks of 12. 2. Each individual was scored according to its response to (i) an approaching human when alone and feeding, (ii) a taxidermist's model fox when alone and feeding, and (iii) an approaching human when alone in a 9 m annulus, and its nearest neighbour distance in the home pen, competitiveness for food in a series of paired encounters, running ability and physical characteristics. 3. Each flock was herded around a 7 m diameter annulus in separate trials by a human walking at 0.5ms(-1). The distance maintained by each duckling from the human, and hence its relative flock position, was calculated. 4. Individual flight distance maintained from an approaching human when alone in an annulus was the attribute that predicted distance maintained from an approaching human when part of a flock. 5. Some ducklings had reduced average distances from an approaching human when they were in a flock compared to when they were tested alone, and inter-individual variation in distance from the human was greatly reduced when birds were part of a flock.     

Effects of atelocollagen gel containing bone marrow-derived stromal cells on repair of osteochondral defect in a dog

To clarify the contribution of autologous transplantation of mesenchymal stromal cells (MSCs), an atelocollagen gel containing or not containing fluorescently-labeled canine MSCs was transplanted into an osteochondral defect which did not repair spontaneously and the histological repair of the defect was compared. Although an early repair of the cartilage was not observed in either defect, the reproduction of subchondral bone was remarkable in the MSCs-implanted defect. Moreover, in 2 weeks after operation, the implanted MSCs were located in the deeper regions of the defect, suggesting the differentiation of osteoblasts. There was a possibility that the movement of the implanted MSCs was due to an increase in intra-articular pressure from postoperative inflammation.     

Epidural myelolipoma in a Husky-cross: a case report

Epidural spinal myelolipoma was diagnosed in an 11.5-year-old castrated male Husky-cross that was evaluated at the veterinary teaching hospital due to progressive thoracolumbar spinal hyperaesthesia and mild proprioceptive pelvic limb ataxia. A focal, ill-defined mildly inhomogenous extradural mass lesion was detected by MRI. The dog was euthanized. At necropsy an extradurally located reddish mass of about 2.5 cm in diameter was present in the vertebral canal. The mass was identified histopathologically as an epidural myelolipoma.     

Cervical Intervertebral Disk Protrusion in a Horse

A Hansen type ll intervertebral disk protrusion was diagnosed in an 18-year-old horse with signs of progressive ataxia and paresis. A soft tissue mass dorsal to the C6-C7 intervertebral disk space was identified at myelogram and found to be an intervertebral disk protrusion with enlarged dorsal annulus fibrosis at surgery. Surgical decompression was achieved using a ventral approach to the caudal cervical vertebrae, an approach limited in its application by the vertebral venous sinuses. Neurological signs remained unchanged for 3 days and then deteriorated. The horse was euthanized.     

Granulosa-theca cell tumor associated with an ovulation fossa and normal ovarian stroma in a mare.

A granulosa-theca cell tumor was found in an ovary that had an ovulation fossa and normal ovarian tissue. The ovary was removed from a mare with a history of ovarian enlargement and behavioral changes. The affected ovary had a multicystic appearance on ultrasonographic examination performed before surgery, and an ovulation fossa was not palpable on examination per rectum. However, during surgery, the affected ovary was found to be within normal size limits, with an enlargement on 1 pole, and to contain an ovulation fossa. Atrophy of the infundibulum of the affected ovary helped to confirm the diagnosis of granulosa-theca cell tumor, and the ovary was removed. The mare's testosterone concentrations were normal. Granulosa-theca cell tumors are usually associated with a spherical ovary, attributable to ablation of the ovulation fossa, with no normal ovarian tissue present.     

Erythrocyte osmotic fragility testing and the prediction of canine malignant hyperthermia susceptibility

A Doberman-German Shepherd cross-bred male dog, previously diagnosed as malignant hyperthermia susceptible, was mated to an unrelated nonsusceptible German Shepherd cross-bred female. The resultant litter was subjected to hematological, biochemical and erythrocyte osmotic fragility testing in an endeavor to predict the susceptibility of individuals to malignant hyperthermia. Laboratory evaluations were repeated at one year of age and the litter subjected to the halothane challenge test. No significant difference in erythrocyte osmotic fragility was found between malignant hyperthermia susceptible and nonsusceptible siblings at six weeks or at one year of age. Erythrocyte osmotic fragility, in both malignant hyperthermia susceptible and nonsusceptible animals, increased between six weeks and one year of age. Dantrolene sodium was an effective treatment for malignant hyperthermia in the dog when administered early in an episode and in adequate dosage. The initial sign of a malignant hyperthermia episode was a very rapid increase in end tidal partial pressure of carbon dioxide. This finding reinforces the value of capnographic monitoring in anesthesia.     

Pathology of the active Arthus reaction in the chicken

It was possible to produce an active Arthus reaction in chicken skin which resulted in gross and microscopic lesions. Histologically, the reaction was predominantly thrombotic in nature and restricted to the upper dermis. The thrombi appeared to develop as a consequence of immune complex deposition with adherence and aggregation of thrombocytes at the vascular endothelium. Thrombosis induced widespread necrosis and haemorrhage and vasculitis occurred in the lower dermis. Up to four hours after inoculation, the cell population comprised an infiltration of heterophils, monocytes and basophils, suggesting an immediate hypersensitivity reaction. This was followed by an Arthus type reaction for four to 12 hours involving both heterophils and monocytes. A characteristic feature was the development of early perivascular lymphoid foci. After 12 hours the reaction resembled a delayed hypersensitivity. The use of colloidal carbon suggested that whereas phagocytic activity of the heterophils and basophils appeared sensitisation dependent, that of thrombocytes and monocytes was independent of it. The findings indicate that in the Arthus reaction in the chicken the thrombocyte appears to be the principal cell producing tissue damage by thrombosis. A comparison was made with the active Arthus reaction in the rabbit.     

Effect of topical phenylephrine on the equine pupil

The mydriatic effect of 10% phenylephrine was evaluated in 9 horses. Base-line pupillary size in mesopic conditions and during light stimulation was ascertained before application of pharmacologic agents. In study 1, 10% phenylephrine was applied to each eye (n = 5 horses). After 15 minutes, the pupillary size was determined in both lighting conditions. Phenylephrine was again applied to each eye, and after an additional 15 minutes, the pupillary size was determined. In study 2, 1% tropicamide was applied to each eye (n = 4 horses), and after 30 minutes, the pupillary size was determined in both lighting conditions. Tropicamide and phenylephrine were applied to each eye immediately after determination of pupillary size, and after an additional 30 minutes, the pupillary size was determined. There was no significant change (P greater than 0.9) in pupillary size when phenylephrine was used alone or when phenylephrine was used in an eye that had been dilated with tropicamide.     

The failure of the oesophageal groove reflex,when fluids are given with an oesophageal feeder to newborn and young calves

The fate of colostrum and other fluids given with an oesophageal feeder was studied in newborn calves and in young calves up to 3 weeks by means of radiological techniques. Any fluid given with an oesophageal feeder was found in the forestomachs, since it apparently did not induce an oesophageal groove reflex. Also when suckling was induced during feeding with the oesophageal feeder, the oesophageal groove reflex did not occur. The outflow of colostrum and other fluids from the forestomachs to the abomasum and small intestine occurred for the most part within 3 hours. Only the emptying of the ventral ruminal sac was sometimes delayed for 24 or 48 hours.

The results obtained in calves up to 3 weeks old did not differ from those obtained in newborn calves.

It was concluded that although colostrum given by oesophageal feeder entered the forestomachs, the rapid flow to the abomasum and small intestine created the conditions for a sufficient absorption of immunoglobulins. Application of colostrum with an oesophageal feeder in newborn calves promises to be a timesaving and effective method for creating an optimum passive immunity of the calves.     

Influence of the number of days spent training in an abattoir with access to live cows on the efficiency of do-it-yourself artificial insemination

Data relating to 35,389 inseminations carried out in the first three years after inseminators were trained were collected by postal questionnaire from 94 do-it-yourself (DIY) artificial inseminators in the UK. The mean calving rate from 14,528 inseminations they carried out on 92 farms in the first year after they were trained was 59.4 per cent (range 5 to 92 per cent). In the second year the mean calving rate from 11,515 inseminations by 64 inseminators was 62.3 per cent (range 33 to 88 per cent), and in the third year the mean calving rate from 9346 inseminations by 49 inseminators was 64.6 per cent (range 41 to 92 per cent). There was an increase of 5.2 per cent in the mean calving rate over the three years with an overall mean calving rate of 61.5 per cent. Each trainee carried out an average of 376 inseminations (range 20 to 800) during the three years. Thirteen instructors were responsible for the tuition and the average time the trainees spent in an abattoir with access to live cows was 2.88 days, with a range from none to five days. For the trainees who spent up to three days training in an abattoir there was an increase of 5.9 per cent in the calving rate they achieved in their first year for every day they spent training with access to live cows (P < 0.005), but there were no significant effects on the outcome of their inseminations in their second or third years.     

Pharmacokinetics of florfenicol in healthy and Escherichia coli-infected broiler chickens

The aims of this study were to evaluate the effects of a ketamine/propofol anaesthetic protocol in lions (Panthera leo), and to compare it to two commonly used anaesthetic protocols. Seventeen adult lions were anaesthetised using three different protocols. Group XK (n=6) was anaesthetised with intramuscular (i.m.) injections of xylazine and ketamine. Group KD (n=5) was anaesthetised with an i.m. injection of ketamine, followed by an intravenous (i.v.) injection of ketamine and diazepam. Group KP (n=6) was anaesthetised with an i.m. injection of ketamine followed by an i.v. injection of propofol. There was a significant difference in heart rate (P<0.0002), which was lowest in group XK and highest in KD. Jaw tone was significantly lower in Group XK (P<0.05). No undesirable effects were noted following injection of the propofol. Propofol was a suitable and safe drug for maintenance of anaesthesia in adult lions.     

Pyloric obstruction secondary to epicardial pacemaker implantation: a case report

A 10-year old Lhasa Apso dog was presented for an acute history of exercise intolerance and hind limb weakness. High grade second degree atrioventricular block with an atrial rate of 200 beats per minute, ventricular rate of 40 beats per minute and an intermittent ventricular escape rhythm, was diagnosed on electrocardiograph. A transdiaphragmatic, unipolar, epicardial pacemaker was implanted without immediate surgical complications. Severe vomiting was noted 12 h post-operatively. Abdominal ultrasound and a barium study supported a diagnosis of pyloric outflow obstruction and exploratory abdominal surgery was performed. The pyloric outflow tract appeared normal and no other causes of an outflow obstruction were identified. The epicardial generator was repositioned from the right to the left abdominal wall. Pyloric cell pacing was presumed to be the cause for the pyloric obstruction and severe vomiting, and this was thought to be due to close proximity of the pacemaker generator to the pylorus situated in the right abdominal wall. Repositioning of the pulse generator to the left abdominal wall resulted in resolution of vomiting.     

Field studies on the efficacy of a long acting preparation of oxytetracycline in controlling outbreaks of enzootic abortion of sheep

The effects of long acting oxytetracycline in controlling outbreaks of enzootic abortion of ewes were studied over three consecutive years in 13 different commercial flocks. When used in the face of an outbreak the compound produced a statistically significant reduction in abortion in treated ewes when compared to untreated controls. There was, however, only a marginal difference in the overall abortion rate between treated and untreated groups of ewes in known infected flocks, where the drug was used in anticipation of an abortion storm, mainly because the abortion rate was too low in the control group. It is suggested that oxytetracycline therapy should only be used in an effort to control an actual abortion outbreak on the understanding that the treatment will not eradicate infection from the flock.     

Multiple anthelmintic resistance in Trichostrongylus colubriformis

Following the failure of anthelmintic treatment to control an outbreak of trichostrongylosis in sheep, multiple resistance to levamisole and oxfendazole was confirmed in field strains of Trichostrongylus colubriformis at the CSIRO Pastoral Research Laboratory, Armidale. Resistance in Trichostrongylus spp to levamisole was also confirmed on an adjoining farm. From the results of an experiment where lambs were treated at the recommended dose rate with one or both anthelmintics, it was estimated that 32% of T. colubriformis were resistant to levamisole, 19% to oxfendazole, and 12% to both drugs. Simultaneous administration of levamisole and oxfendazole resulted in an additive anthelmintic effect. Naphthalophos (36.6 to 51.2 mg/kg) was 93% efficient against the multiple resistant strain. The similar histories of anthelmintic usage at the CSIRO Pastoral Research Laboratory and on the northern tablelands of New South Wales generally, suggest that multiple anthelmintic resistance in T. colubriformis may soon emerge as a problem on the northern tablelands.     

Inhibition of elastase by canine serum: demonstration of an acute phase response

Canine serum alpha 1-proteinase inhibitor is not a major acute phase reactant in the dog, unlike the equivalent protein in humans. The possibility that an alternative protease inhibitor system is stimulated during the acute phase response in the dog was investigated. alpha 2-macroglobulin was not an acute phase reactant, but an inhibitor of elastase was identified in canine serum which could be separated from proteinase inhibitor by gel filtration and which was shown to be an acute phase reactant. This protein has been named canine elastase inhibitor.     

Evaluation of an Esophageal Stethoscope as an Early Predictor of Serious Cardiovascular Insufficiency in the Dog

Accuracy of an esophageal stethoscope combined with a vital signs monitor (VSM) was evaluated as an early predictor of cardiovascular failure during anesthesia. In 10 anesthetized dogs, hypotension and reduction in cardiac output were induced by gradually increasing the inspired halothane concentration. Cardiovascular performance and intensity of heart sounds recorded by the esophageal stethoscope and amplified by the VSM were assessed as depth of anesthesia was increased. Halothane concentration was increased until cardiovascular performance was reduced to a point where heart sounds were no longer audible from the VSM. The esophageal stethoscope in combination with the VSM was easy to use and the unit had an excellent signal to noise ratio. However, usefulness of the esophageal stethoscope as an early predictor of serious cardiovascular depression could not be substantiated. In three dogs, cardiac arrest occurred simultaneously with the loss of heart sounds and in the remaining seven dogs, sound loss occurred when the degree of cardiovascular depression was life threatening.     

内流质子梯度对Gly-Pro在仔猪小肠跨膜转运中的驱动作用

为研究内流质子梯度对仔猪小肠中Gly-Pro跨膜转运的影响,采用放射性同位素示踪及体外孵育技术,观察在不同H＋浓度条件下,Gly-Pro在仔猪小肠刷状缘膜囊（Brush border membrane vesicles,BBMV）的跨膜转运量。结果表明：膜囊内液pH为7.5时,Gly-Pro的转运显著地受到膜囊外液pH值的影响,pH为4.5～5.5时,转运最快;其中pH为5.0时的Gly-Pro跨膜转运速度在1-20 min一直高于pH为7.5时的转运速度;内流质子梯度的存在可以显著促进Gly-Pro的转运,无内流质子梯度时,Gly-Pro的转运不受膜囊外液pH变化的影响。研究结果提示,跨膜内流质子梯度是仔猪小肠中Gly-Pro的跨膜转运的一种驱动力。     

Hyperbilirubinemia in sick cattle.

Hyperbilirubinemia was present in 387 out of 1279 sick cattle admitted to the clinic of the Ontario Veterinary College. One hundred and ninety five of these had a total serum bilirubin of 17 mumol/L or greater. Clinical records, laboratory data and when available, pathology reports from these 195 animals were examined in an attempt to explain the reason for the high bilirubin levels. The hyperbilirubinemia in 187 of these was mainly due to an increase in unconjugated bilirubin. Jaundice, liver disease or anemia was not a feature and alkaline phosphatase was not elevated. The 195 animals with hyperbilirubinemia suffered from a variety of diseases that seemingly were unrelated to the increased bilirubin. The most frequently described signs were anorexia and rumen stasis. Liver disease was diagnosed in eight animals and in these clinical jaundice, and increased conjugated bilirubin and alkaline phosphatase was a feature. It was concluded that hyperbilirubinemia occurred in many diseases of cattle and in most instances was related to a failure of the liver to remove unconjugated bilirubin from the serum rather than to a failure of the liver to excrete conjugated bilirubin.     

Clinical comparison of isoflurane and sevoflurane anaesthesia in the gerbil (Meriones unguiculatus)

The objective of this study was a comparison of the volatile anaesthetics isoflurane and sevoflurane in terms of their clinical effects in gerbils (Meriones unguiculatus) (n=12 each). Induction of anaesthesia was performed in a body chamber with an anaesthetic concentration of 4.0 Vol.% at an oxygen flow of 500 ml/min for isoflurane and 8.0 Vol.% at an oxygen flow of 1000 ml/min for sevoflurane, respectively. Anaesthesia was maintained via nose cone with an anaesthetic concentration of 2.8 to 3.2 Vol.% at an oxygen flow of 200 ml/min for isoflurane and 5.0 to 5.2 Vol.% at an oxygen flow of 400 ml/min for sevoflurane. Those anaesthetic concentrations ensured reflex status conform with surgical tolerance. In spite of its higher blood-gas coefficient induction time was slightly faster for isoflurane. Recovery time was significantly longer in the isoflurane group than it was in the sevoflurane group. Both inhalants caused respiratory depression. Respiratory rate was lower in sevoflurane animals compared to isoflurane. The animals were positioned on a heating pad immediately after induction, thus a decrease of the body temperature could be prevented. Both inhalants can be recommended for usage in gerbils. Sevoflurane showed no clinical benefit compared to isoflurane.