甘氨酸铁对断奶仔猪血清氧化酶活力及生化指标的影响

试验研究甘氨酸铁对断奶仔猪抗氧化能力及血清生化指标的影响,并初步探讨其作用机理。选用180头体重7.5kg左右的"杜长大"三元杂交仔猪,分为6个处理组,分别饲喂0、30、60、90、120mg/kg甘氨酸铁和120mg/kg硫酸亚铁,试验期5周。结果表明:饲粮中添加甘氨酸铁,均不同程度的提高了血清中SDH、CAT、XOD和AKP的酶活,降低了血尿氮(SUN)、Ca和P含量。由此可知,甘氨酸铁可以影响仔猪机体抗氧化能力和血清生化指标,在一定程度上改善仔猪的生产性能。     

The effects of dietary tomato powder (Solanum lycopersicum L.) supplementation on the haematological,immunological, serum biochemical and antioxidant parameters of growing rabbits

Using nutritional antioxidants in livestock systems is considered the key in improving animal production. The current study assumes that dietary tomato powder (TP) supplementation positively affects haemato‐immunological, biochemical, and antioxidant parameters for New Zealand rabbits. A total of 30 rabbits (45 days old) were assigned to three groups, including a diet with no additives (control), and two dietary treatments with the providing of 1% or 2% TP. Mass spectrometric study for TP methanolic extract showed some phenolic compounds. Consumption of TP supplemented diets significantly (p < 0.001) affected body weight gain and feed efficiency. Red blood cells and white blood cells count exhibited a significant increase (p < 0.001) in both TP groups compared with the control. In addition to, feeding rabbits on TP enhanced cell‐mediated and humoral immune responses through a significant increase in phagocytosis, chemotaxis, and levels of immunoglobulins (TIg, IgG, IgM and IgA). Supplementation of TP significantly (p < 0.01) reduced lipid profile induces except high‐density lipoprotein cholesterol values. A remarkable significant (p < 0.001) effect on serum and hepatic oxidative stress responses were observed with TP addition. Ultimately, TP supplementation could play a potential role as a growth and health enhancer for fattening rabbits.     

Effect of low‐ or high‐CP diet with cold‐pressed oil supplementation on growth,immunity and antioxidant indices of growing quail

This study was to investigate the effect of dietary protein levels and supplementation with two cold‐pressed oils on the performance, immunity and antioxidant indices of growing Japanese quails. A 3 × 4 factorial experiment, using 3 dietary crude protein (CP) levels (22%, 24% and 26%) and 4 supplemental herbal oils levels: control without additives, 250 mg marjoram (Mar), 250 mg thyme (Thy) and 125 mg Mar + 125 mg Thy per kg diet. A total of 720 unsexed, 7‐day‐old quail chicks were randomly distributed into twelve treatment groups (4 replicates per group of 15 chicks in each). The live body weight (LBW) on 42 days and body weight gain (BWG) from 7 to 42 days were significantly improved (p < 0.01 and 0.05) in chicks fed 24% or 26% CP compared with those of the 22% CP group. The groups fed diets supplemented with Mar or in combination with Thy oil exhibited significantly better LBW, BWG and feed conversion ratio (FCR) than the control at all periods except BWG from 21 to 42 days and FCR from 7 to 21 days were not significant. Feed intake was gradually decreased with increasing dietary CP level or adding herbal oils during the periods 21–42 and 7–42 days of age. Plasma triglycerides, cholesterol, total lipids and malondialdehyde were decreased by cold‐pressed oils supplementation, but the activity of reduced glutathione and superoxide dismutase was increased (p < 0.01) compared with the control. In conclusion, quails fed a diet containing 24% or 26% CP or diets supplemented with Mar or Thy oils or both exhibited improvement in the performance, lipid profile, immunity and antioxidant capacity without any detrimental impacts on the other studied parameters.     

太子参参须提取物对小鼠血清免疫指标和抗氧化指标的影响

研究太子参参须提取物(RPFRE)对小鼠血清免疫指标和抗氧化指标的影响。将40只雄性昆明小鼠随机分为空白对照组、RPFRE低剂量组(100 mg/kg)、RPFRE中剂量组(200 mg/kg)和RPFRE高剂量组(400 mg/kg),给空白对照组小鼠灌胃蒸馏水,给RPFRE组小鼠分别灌胃相应剂量的RPFRE,连续2周。第15天,采集小鼠血液,检测血清抗氧化指标(T-AOC、SOD、MDA)和免疫指标(免疫球蛋白IgG、IgM、IgA及补体C₃、C₄)。结果显示,与对照组相比,RPFRE高剂量组小鼠IgG含量、低剂量组IgM含量、中剂量组补体C₃含量均显著升高(P<0.05);3个剂量RPFRE组T-AOC极显著升高(P<0.01)。说明RPFRE能不同程度提高小鼠免疫功能和抗氧化功能。     

油菜素内酯对干旱胁迫下羊草渗透调节及抗氧化酶的影响研究

受全球气候变化影响,我国牧区干旱现象日益严重,极大限制了草原生产力的提高。本文采用盆栽人工控水的方法,研究不同浓度油菜素内酯(BR)处理对羊草生长和抗旱特性的影响。结果表明干旱显著抑制植株的生长,而BR能有效减缓干旱胁迫对羊草造成的伤害。经过不同浓度的BR处理后,与干旱胁迫相比,株高、叶面积、干重、含水量、叶绿素a、叶绿素b、类胡萝卜素、根系活力、脯氨酸、可溶性蛋白、可溶性糖和5种抗氧化酶活性均随浓度增加而升高,但高浓度下又有降低的趋势,而丙二醛(MDA)和叶片电导率则相反,其中以BR浓度为0.1 mg/L时的抗旱效果最好。研究发现适当浓度的BR提高羊草株高、叶面积和光合色素含量,促进干物质积累和根系活力,与其降低膜脂过氧化产物MDA与质膜透性,提高脯氨酸、可溶性蛋白、可溶性糖等渗透调节物质含量,增强超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)、抗坏血酸过氧化物酶(APX)、谷胱甘肽还原酶(GR)等抗氧化酶活性密切相关。     

酸胁迫对夏枯草叶绿素荧光特性和根系抗氧化酶活性的影响

为了阐明夏枯草幼苗对酸性环境的耐受性,采用不同pH值(pH=4.5、4.0、3.5、3.0)的Hoagland营养液模拟酸胁迫环境,研究酸胁迫对夏枯草幼苗叶绿素荧光特性及根系抗氧化酶活性的影响。结果表明,随着pH值下降,代表供体与受体侧参数的K点相对可变荧光(W_K)、J点相对可变荧光(V_J)和电子受体QA-被还原的最大速率(dV/dt_o)逐渐升高,光系统Ⅱ(PSⅡ)电子传递的量子效率(φE_o)降低;表示单位反应中心吸收(ABS/RC)、捕获(TR_o/RC)、热耗散(DI_o/RC)的能量逐渐增加;用于电子传递的能量(ET_o/RC)逐渐下降;表示PSⅡ的最大光化学效率(F_v/F_m)、吸收光能为基础的光合性能指数(PI_abs)、表征光系统Ⅰ(PSⅠ)的最大氧化还原活性(ΔI/I_o)及PSⅡ与PSⅠ协调性(Φ_PSⅠ/PSⅡ)随酸胁迫程度增加逐渐下降。夏枯草根系超氧化物歧化酶(SOD)与过氧化物酶(POD)活性呈下降趋势,而根系抗坏血酸过氧化物酶(APX)活性先升高后降低;夏枯草叶片可溶性糖、脯氨酸含量逐渐上升。酸胁迫下,夏枯草PSⅡ反应中心受损,电子传递受阻,光系统性能及PSⅡ与PSⅠ的协调性降低,根系SOD与POD活性受到抑制,在pH 3.0处理下表现较为明显。夏枯草通过提高单位反应中心的热耗散能力来减少过剩光能对叶片光合机构的破坏,通过提高根系APX抗氧化酶及叶片渗透调节物质含量来减少酸胁迫带来的伤害。     

不同属种肉类对大鼠血清生化指标与抗氧化能力的影响

探讨了不同属种肉类对大鼠血清生化指标和抗氧化机能的影响。试验选取体重约177 g的雄性SD大鼠90只,随机分为3组即狗肉组、羊肉组、鸭肉组,分别将日粮中的主要蛋白源替换为狗肉、羊肉、鸭肉(CP 31%)。试验期为4周。结果表明:饲喂狗肉和羊肉能显著提高大鼠生长性能,且前者使得大鼠血清碱性磷酸酶(ALP)活性极显著升高(P<0.01);而饲喂鸭肉使得大鼠血清丙氨酸转移酶(ALT)活性显著升高(P<0.05),天冬氨酸转移酶(AST)、血清尿素氮(BUN)活性极显著升高(P<0.01)。不同属种的肉之间,鸭肉组肝脏和肾脏中谷胱甘肽过氧化物酶(GSH-Px)活性均极显著升高(P<0.01)。结果提示:饲喂狗肉和羊肉能促进机体蛋白质合成,有利于蛋白质沉积;饲喂鸭肉则具有增强机体抗氧化的能力。     

盐碱胁迫对寸草苔渗透调节物质含量和抗氧化酶活性的影响

以分布于科尔沁沙地的粉煤灰草地,盐碱地和荒地3种不同生境的野生寸草苔为材料,通过蛭石营养液培养,进行混合盐碱胁迫处理,检测其生理生化变化。结果表明：3种生境寸草苔的相对电导率和丙二醛（MDA）含量随着盐碱胁迫处理浓度的增大而增加;盐碱地寸草苔的超氧化物岐化酶（SOD）和过氧化物酶（POD）活性在300mmol/L高浓度盐碱胁迫增加明显,荒地寸草苔和粉煤灰草地寸草苔则变化不明显,过氧化氢酶（CAT）活性则随着盐碱胁迫浓度增大呈现先升高后降低的变化趋势,3种抗氧化酶的活性均以盐碱地寸草苔最强;可溶性糖和脯氨酸两种渗透调节物质的含量,随着盐碱胁迫浓度的增大而增加,尤以高浓度处理增加显著。     

Evaluation of haematological,serum biochemical and histopathological parameters of growing rabbits fed Amaranthus dubius

Amaranthus dubius is an alternative source of fibre and protein for the feed formulation for growing rabbits, although its effects on their health status are yet unknown. Therefore, this study aimed to evaluate the effects of three dietary levels (0, 160 and 320 g/kg) of A. dubius on haematological, serum biochemical and histopathological parameters of fattening rabbits. One hundred and thirteen New Zealand White rabbits were fed ad libitum from weaning (35 days) to slaughter (87 days). The health status of the animals was monitored, and mortality was recorded. Before slaughtering, 30 rabbits were randomly selected for haematological and serum biochemical analysis. During slaughter, 18 rabbits were randomly selected for histopathological study in heart, liver, lung and small intestine. The health status of rabbits was good, with no differences among treatments in mortality. Diet and sex did not affect the values of most haematological and serum biochemical parameters. Fibrinogen, glucose and high‐density lipoprotein values increased significantly from 0 to 320 g/kg A. dubius diets. The white blood cell count and glutamic oxaloacetic transaminase values decreased significantly from 0 to 320 g/kg A. dubius diets. The organs showed histologically well‐structured tissues. However, in all experimental groups several samples with lesions were observed, always highly focalized in small tissue areas. The main lesions observed, probably related to conditions of thermal stress affecting the rabbits, were cholesterolosis (heart), steatosis (liver), inflammatory lymphoplasmacytic infiltrate (lung) and chronic inflammatory tissue damage (small intestine). In conclusion, the inclusion of up to 320 g/kg A. dubius in diet did not jeopardize the health status of growing rabbits. Therefore, A. dubius can become an alternative raw material for the formulation of feeds in tropical and subtropical countries where this plant is widespread.     

Effects of lycopene supplementation in both maternal and offspring diets on growth performance,antioxidant capacity and biochemical parameters in chicks

This study investigated the effects of different supplementation ways of lycopene during pre‐hatch (from the diet of hens) and post‐hatch (from the diet of progeny) on production performance, antioxidant capacity and biochemical parameters in chicks. In total, 360 hens were fed diets supplemented with 0 (control group) or 40 mg lycopene/kg diet. From 28 to 34 days after the start of supplementation (30 weeks old), 650 qualified eggs were collected to artificial incubation. In this trial, 2 × 2 factorial designs were used. Male chicks hatched from hens fed with 0 or 40 mg lycopene/kg diet were fed a diet containing either 0 or 40 mg lycopene/kg diet. The results showed that, relative to control, in ovo‐deposited lycopene significantly increased chick birth body weight, improved liver total antioxidant capacity (T‐AOC), glutathione peroxidase (GSH‐Px) and glutathione to oxidized glutathione ratio (GSH: GSSG), and significantly declined liver malondialdehyde (MDA) level and increased liver lycopene content during 0–14 days after hatching. On days 14 after hatching, dietary lycopene in diet began to take over gradually. Both supplementation ways of lycopene increased immune organ index, serum high‐density lipoprotein (HDL) cholesterol, villus length and villus/crypt in duodenum, jejunum and ileum. Data in this study suggested lycopene supplementation could improve antioxidant capacity and immune function, and regulate lipid metabolism in chicks.     

Blood glutathione status and activity of glutathione‐metabolizing antioxidant enzymes in erythrocytes of young trotters in basic training

The aim of this study was to evaluate response of blood glutathione status and activity of glutathione‐metabolizing antioxidant enzymes in erythrocytes of young trotters in basic training. Nine untrained trotters (aged 16–20 months) were exposed to a 4‐month training program based on exercises at low‐to‐moderate intensity. The conditioning consisted of breaking the horses and running them on distances varying from 4 to 40 km a week. The workloads were increased on a 3‐week basis. Exercise intensity was monitored by measuring heart rate and blood lactate. Blood samples were collected at rest, before (RES0) and after (RESt) the conditioning period; moreover, on the latter occasion (on day 112 of training), the blood was also taken immediately after the routine exercise (EXE0) and 60 min thereafter (EXE60). The whole blood samples were analysed for the concentration of reduced, oxidized and total glutathione (GSH, GSSG and TGSH, respectively), while the activities of glutathione peroxidase (GPX) and glutathione‐disulfide reductase (GR) were determined in haemolysates. Additionally, the erythrocytic concentrations of oxidized nicotinamide adenine dinucleotide (NAD⁺) and its phosphate (NADP⁺) were measured. All investigated parameters except NAD⁺ and reduced/oxidized glutathione ratio (GSH/GSSG) changed during the training period. Following the effortm GPX, NADP⁺ and GSH/GSSG were significantly lower (p < 0.05, p < 0.01, p < 0.001, respectively) while GSSG was markedly higher than at rest (RESt). The drop in NADP⁺, low GSH/GSSG and high GSSG concentration were sustained at EXE60. Glutathione‐disulfide reductase activity was higher after the workout but only at EXE60 the increase in activity was significant. Despite the activities of the GSH‐GSSG cycle, enzymes were considerably higher after the training period, the elevated concentration of GSSG and significantly lower GSH/GSSG ratio in the post‐exercise measurements suggest that production of reactive oxygen species possibly exceeds the capacity of antioxidative defenses of immature trotters. A more balanced diet with additional antioxidant supplementation and a revision of the basic training protocol used herein are advised.     

五味子提取物对断奶仔猪血液生化指标和抗氧化能力的影响

本试验通过测定断奶仔猪血清、心脏、肝脏、肾脏和脾脏组织中超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量和总抗氧化能力(T-AOC)以及血清生化指标,研究五味子提取物对断奶仔猪血液生化指标和抗氧化能力的影响。结果显示,五味子提取物能够增强断奶仔猪的抗氧化能力。     

Effects of essential oil supplementation of a low‐energy diet on performance,intestinal morphology and microflora,immune properties and antioxidant activities in weaned pigs

A total of 144 weaned piglets were used to evaluate the effects of essential oil (EO) supplementation of a low‐energy diet on performance, apparent nutrient digestibility, small intestinal morphology, intestinal microflora, immune properties and antioxidant activities in weaned pigs. Pigs received a low‐energy diet (negative control, NC, digestible energy = 3250 kcal/kg), NC plus 0.025% EO or a positive control diet (PC, digestible energy = 3400 kcal/kg) for 28 days. Growth performance was similar between the EO group and PC group. However, EO supplementation increased (P < 0.05) average daily gain and the apparent digestibility of dry matter, crude protein and energy compared with pigs fed the NC diet. Greater (P < 0.05) villus height and lower (P < 0.05) counts of Escherichia coli and total anaerobes in the rectum in the EO group were observed compared with NC or PC groups. Pigs fed EO diet had higher (P < 0.05) concentrations of albumin, immunoglobulin A (IgA), IgG and total antioxidant capacity and lower fecal score than pigs fed the PC and NC diets. Above all, this study indicates that supplementation of EO to a low‐energy pig diet has beneficial results and obtains similar performance compared with normal energy (PC) diet.     

Effects of alpha‐lipoic acid supplementation on growth performance,antioxidant capacity and biochemical parameters for ammonia‐exposed broilers

In order to estimate the effect of alpha‐lipoic acid (LA) supplementation on relieving ammonia stress of broilers, 180 22‐day‐old male broilers were assigned to three groups, six replicates in each group and 10 birds per replicate. The three groups were: (1) a control group without ammonia stress; (2) exposure to 70 ppm atmospheric ammonia (AM); (3) exposure to 70 ppm atmospheric ammonia and administration of 300 mg/kg LA (AM + LA). The experimental period was 3 weeks. Results showed that average daily weight gain was increased and feed conversion ratio was decreased in the AM + LA group, compared with the AM group (P < 0.05). Total superoxide dismutase and glutathione peroxidase activities in serum, and glutathione content in liver were higher in the AM + LA group than that in the AM group (P < 0.05); however, serum malondialdehyde content was decreased by LA addition (P < 0.05). Additionally, serum glutamic‐pyruvic transaminase, creatine kinase and lactate dehydrogenase activities were reduced and albumin level was increased by LA addition (P < 0.05). In conclusion, LA addition could relieve ammonia stress to restore broiler production performance to normal levels.     

Effects of ketamine‐diazepam and ketamine‐acepromazine combinations on intraocular pressure in rabbits

ObjectiveTo determine the effects of ketamine-diazepam and ketamine-acepromazine combinations on intraocular pressure (IOP) in rabbits.Study designRandomized clinical trial.AnimalsSixteen adult New Zealand white rabbits approximately one year old, weighing 2.3 ± 0.2 kg were used in this study.MethodsThe animals were randomly divided into two groups of eight each (KA and KD). The pre-treatment IOPs were recorded in both groups (T0). All rabbits in group KA received intramuscular ketamine-acepromazine (ketamine 30 mg kg^?1+ acepromazine 0.5 mg kg^?1). Ketamine-diazepam (ketamine 30 mg kg^?1 + diazepam 1 mg kg^?1) was administered intramuscularly in members of group KD. The IOP values were measured at 5 (T₅), 15 (T₁₅), and 20 (T₂₀) minutes after drug administration in both treatment groups.ResultsSignificant increases in IOP values were observed in both treatment groups at T_5, T₁₅, and T₂₀ in comparison to the baseline values. In group KA the mean ± SD IOP at T_5, T₁₅, and T₂₀ were 37 ± 13 (p < 0.001), 35 ± 4 (p < 0.001) and 34 ± 4 mmHg (p < 0.001). The post-treatment mean ± sd values in group KD were 23 ± 8 (p = 0.002), 23 ± 5 (p < 0.001) and 23 ± 6 mmHg (p = 0.001) at 5, 15, and 20 minutes respectively.Conclusion and clinical relevanceBoth ketamine-diazepam and ketamine-acepromazine combinations increased IOP after intramuscular administration in rabbits.     

添加不同水平复合中草药对蛋鸡血清、组织以及鸡蛋抗氧化指标的影响

为研究不同水平复合中草药对蛋鸡血清、组织和鸡蛋抗氧化指标的影响,试验选用120日龄健康、体重相近的海兰褐蛋鸡300只,随机分为5组,每组6个重复,每个重复10只鸡。对照组饲喂基础日粮,试验组分别在基础日粮中添加0.4%、0.3%、0.2%、0.1%的复合中草药。试验期40d。结果表明:(1)与对照组相比,添加复合中草药制剂能够显著提高蛋鸡平均蛋重和产蛋率(P<0.05),添加0.1%、0.3%和0.4%复合中草药组平均蛋重分别提高6.4%、4.4%和6.0%(P<0.05);添加0.3%复合中草药组产蛋率显著提高22.2%(P<0.05);(2)添加0.3%和0.4%复合中草药组超氧化物歧化酶(SOD)活性分别提高5.5%和5.9%(P<0.05);(3)添加0.4%复合中草药组肝脏SOD含量和总抗氧化能力分别提高56.0%和37.0%(P<0.05);(4)添加0.2%、0.3%和0.4%复合中草药组鸡蛋SOD酶活性分别提高8.3%、18.0%和25.2%(P<0.05)。综上所述,在本试验条件下,饲粮中添加复合中草药能够改善蛋鸡生产性能和蛋鸡血清、组织和鸡蛋抗氧化能力,以添加0.4%的复合中草药制剂效果较好。     

低营养水平日粮添加复合酶对蛋鸡生产性能、蛋品质和抗氧化的影响

本研究旨在探讨不同营养水平日粮添加复合酶对蛋鸡生产性能、蛋品质、抗氧化及盲肠微生物含量的影响.试验选择产蛋率接近的42周龄蛋鸡504只,随机分为3组,每组4个重复,每个重复42只.对照组饲喂正常蛋白能量日粮,T1组饲喂低蛋白能量日粮,T2组饲喂低蛋白能量日粮+100?IU/kg复合酶.在为期9周的饲养试验后测定相关指标...     

Effects of juniper essential oil on growth performance,some rumen protozoa,rumen fermentation and antioxidant blood enzyme parameters of growing Saanen kids

This study aimed to evaluate the effects of juniper essential oil on the growth performance, rumen fermentation parameters, rumen protozoa population, blood antioxidant enzyme parameters and faecal content in growing Saanen kids. Thirty‐six male Saanen kids (36 ± 14 days of age) were used in the study. Each group consisted of 9 kids. The control group (G1) was fed with a diet that consisted of the above concentrated feed and oat hay, whereas the experimental groups consumed the same diet but with the concentrated feed uniformly sprayed with juniper essential oil 0.4 ml/kg (G2), 0.8 ml/kg (G3) or 2 ml/kg (G4). There were no differences (p > 0.05) in live weight, live weight gain or feed consumption between the control and experimental groups. There was a significant improvement (p < 0.05) in feed efficiency in the G3 group. There were no differences in the rumen pH, rumen volatile fatty acid (VFA) profile or faecal pH of the control and experimental groups. The rumen NH₃N values were similar at the middle and end of the experiment, but at the start of the experiment, the rumen NH₃N values differed between the control and experimental groups (p < 0.05). The faecal score value was significantly (p < 0.05) decreased in the experimental groups. The addition of juniper essential oil supplementation to the rations caused significant effects on the kids’ antioxidant blood parameters. Although the superoxide dismutase (SOD) activity, total antioxidant capacity (TAC) and catalase values were significantly (p < 0.05) increased in the experimental groups (G2, G3 and G4), especially group G4, the blood glutathione peroxidase (GPX) value significantly decreased in the experimental groups. The results of this study suggest that supplementation of juniper oil is more effective on antioxidant parameters than on performance parameters and may be used as a natural antioxidant product.     

中草药复方对断奶仔兔免疫性能及抗氧化性能的影响

为研究中草药复方对断奶仔兔免疫性能及抗氧化性能的影响,试验选取体重相近、健康的30日龄伊拉兔断奶仔兔60只,公母各丰,随机分为2组,每组3个重复,每个重复10只.A组为对照组,饲喂基础日粮,B组为试验组,饲喂基础日粮+1％中草药复方,预试期5d,试验期60 d.试验30 d和60 d时,分别采集仔兔血液和免疫器官,测定...     

干旱胁迫和复水对锡金微孔草抗氧化酶系统的影响(简报)

用盆栽法研究了干旱胁迫和复水对锡金微孔草抗氧化酶系统的影响。结果显示,锡金微孔草暂时萎焉期和永久萎焉期土壤含水量分别为7.06%和6.20%;处于暂时萎焉期的锡金微孔草复水后吸水迅速,萎焉状态逐渐消除,复水6 h后处理组外貌与对照基本一致;SOD活性在胁迫处理期间与对照相比无显著变化,复水后有一定幅度的上升;CAT和POD活性在胁迫期间先上升后下降,均显著高于对照水平,且复水后仍然保持较高的活性;MDA含量随着干旱胁迫处理时间的延长而迅速增加,到胁迫第8天时达到最大值,复水后叶片中MDA含量迅速下降,到复水后第5天,胁迫组叶片中MDA含量已接近对照水平。综合各生理生化指标的变化情况来看,锡金微孔草具有较强的抗旱能力。