N-甲基-D-天冬氨酸对培养的大鼠腺垂体细胞分泌生长激素影响的研究

应用细胞培养,结合放射免疫测定法探讨了N 甲基D 天冬氨酸(NMDA)对培养的大鼠腺垂体细胞分泌生长激素(GH)的影响。试验发现:在基础培养液中分别添加浓度为1×10-8,1×10-6和1×10-4 mol/L的NMDA,生长激素比对照组分别提高了67.92%,87.46%和100.67%,差异显著(P<0.05)。结果表明,NMDA能直接刺激腺垂体细胞分泌GH,并与NMDA呈剂量依赖关系。     

N-甲基-D-天冬氨酸对猪生长激素和生长抑素动态分泌模式及特征的影响

本试验通过在日粮中添加一定水平的N-甲基-D-天冬氨酸(NMDA)研究其对猪生长激素(GH)和生长抑素(SS)动态分泌模式及特征的影响.选取体重为(53.0±3.6)kg的杜长大三元杂交猪60头,随机分为2组,每组3个重复,每个重复10头猪(公母各1/2,公猪已阉割),分别饲喂添加0、100 mg/kg NMDA的日粮,猪体重接近90 kg时结束试验,分析猪的生长性能并测定GH和SS.结果显示,在日粮中添加100 mg/kg的NMDA,猪的日增重显著增加(P<0.05),料重比显著降低(P<0.05);GH分泌的总体水平、基线水平和峰强度显著提高(P<0.05).从性别角度而言,阉公猪GH分泌总体水平增加36.91%(P<0.05);基线水平提高了55.69%(P<0.05);母猪GH分泌总体水平提高了45.95%(P<0.05);基线水平提高了50.10%(P<0.05).100 mg/kg NMDA组阉公猪与母猪SS的分泌水平有所提高,但与对照组差异不显著(P>0.05).以上结果表明:日粮添加100 mg/kg的NMDA可以促进猪的生长、提高猪GH的分泌水平,使GH分泌表现出较强的脉冲分泌规律;而NMDA对SS的分泌模式和分泌水平未产生明显影响.     

N-甲基-D-天冬氨酸的研究进展

近年来研究表明 ,N 甲基 D 天冬氨酸 (NMDA)对神经和内分泌具有重要的调控作用。适量的NMDA能够显著促进动物腺垂体中生长激素 (GH)、黄体生成素 (LH)、促性腺激素释放激素 (GnRH)以及催乳素 (PRL)的分泌。本文对NMDA的结构、分布、生物合成、检测方法以及生物学功能等作一简要综述 ,并对其在畜牧业上的应用前景作一展望     

半胱胺对生长猪生长激素脉冲释放及有关代谢激素的影响

采用慢性颈静脉血管瘘管连续采样方法研究了半胱胺 (cysteamine,CS)对生长猪生长激素及有关代谢激素的调节作用。 (1) 4头去势二花脸生长公猪 ,1次口服 CS(80 m g/ kg)后 1、4、7d,GH有极相似的分泌模式。处理后 4h,GH分泌开始加强 ,第 4天总体水平、基础水平进一步提高 ,分别为对照期的 148.90 % (P<0 .0 5 )和 112 .0 9% (P<0 .0 1) ,峰强度也提高 99.35 % (P<0 .0 5 ) ,第 7天 GH分泌总体水平、基础水平、峰强度均下降 ,并接近第 1天水平。(2 ) 6头去势二花脸生长公猪 ,口服 CS(80 mg/ kg)后 4d,血浆 SS水平下降 2 3.40 % (P<0 .0 5 ) ,β-内啡肽 (β- endorphine,β- END)增加 46 .44 % (P<0 .0 5 )。 GH分泌模式与对照期未见有差别。 GH总体水平、基础水平、峰强度明显提高 ,而峰频率及持续时间无明显变化。与此同时 ,T3 、T4含量分别提高 93.0 2 % (P<0 .0 5 )和 6 0 .45 % (P<0 .0 5 ) ,胰岛素含量升高34 .91% (P<0 .0 5 ) ,胃泌素含量无显著变化。结果表明 ,CS能耗褐 SS并提高β- END水平 ,促进 GH、T3 、T4和胰岛素分泌 ,而胃泌素未见明显变化     

对氨基苯胂酸对肥育猪生长性能和肉质的影响及其机理研究

1 2 0头“长嘉 (兴 )”肥育猪按体重、性别和遗传基础分成对照组和试验组 (每组三个重复 ,每个重复2 0头 ) ,分别饲喂含对氨基苯胂酸 (PAPAA)为 0和 70mg/kg的饲粮 ,进行 80天饲养试验 ,随后屠宰测定。结果表明 ,添加PAPAA使日增重提高 8.7% (P <0 .0 5) ,饲料转化率提高 1 1 .5% (P <0 .0 1 ) ,胴体瘦肉率提高 6.7% (P <0 .0 5) ,脂肪率降低 3.5% (P <0 .0 5) ,并发现眼肌面积提高 9.3% (P <0 .0 5)、大理石纹评分提高 5.0 % (P <0 .0 5)。残留测定显示 ;停药 4天后肌肉中砷含量达到国家卫生标准。组织分析发现 :饲粮中添加PAPAA使猪血清生长激素 (GH)浓度升高 4 4.0 % (P <0 .0 5)、胰岛素降低 2 3.4 % (P <0 .0 5) ;背最长肌、肾上腺和腺垂体cAMP含量分别提高 4 8.6%、30 .3%和 57.5% (P <0 .0 3)。上述结果揭示PA PAA似通过影响猪的内分泌系统机能而发挥其促进生长和改善胴体组成的效果     

肾上腺素能α2受体对二花脸猪生长激素分泌的作用

给 6头去势二花脸公猪一次注射肾上腺素能α2 受体激动剂可乐宁 ( Clonidine,6μg/ kg)后 ,GH总体水平、基础水平、峰强度明显升高 ,分别增加 10 4 .10 % ( P<0 .0 1)、113.4 0 % ( P<0 .0 1)和 5 4 .5 0 % ( P<0 .0 1) ,并保持脉冲式分泌 ,但峰值出现在注射后 6 0 min左右 ,明显提前。饲喂半胱胺 ( CS)后第 4天静脉注射 Clonidine,则 GH分泌比对照组显著提高 ,但与单独注射 Clonidine和单独口服 CS相比较未见有显著差异 ( P>0 .0 5 )。结果表明 ,α2 受体激动剂 Cloni-dine可促进 GH分泌 ,且峰值出现时间明显提前     

甲基供体促仔猪生长的内分泌机制的探讨

试验将 72头始重为 1 0kg左右的杜长嘉仔猪随机分为 3组 ,以添加和不添加蛋氨酸为对照 ,试验组喂以添加 80 0mg/kg的甜菜碱。 3组的基础饲粮组成及营养水平相同 ,试验期为 3 6天。结果表明 ,与不添加蛋氨酸组相比 ,添加甜菜碱组猪的日增重和采食量分别提高了 1 1 .82 %(P <0 .0 5)和 8.71 %(P <0 .0 5) ,饲料转化率提高了 2 .79%(P <0 .0 5) ;与添加蛋氨酸组相比无显著差异 (P >0 .0 5)。此外 ,甜菜碱组肝脏中甜菜碱高半胱氨酸甲基转移酶 (BHMT)活较不添加蛋氨酸组高 61 .4 1 %(P <0 .0 5) ,较添加蛋氨酸组高 55.3 6%(P <0 .0 5) ,血样分析结果显示 ,添加甜菜碱组猪比不添加蛋氨酸组显著降低了血清尿素氮含量 (P <0 .0 1 )。提高了血清总铁结合力、GH和IGF -1水平 (P <0 .0 5)。但与添加蛋氨酸组相比 ,差异均不显著 (P >0 .0 5)。上述结果提示 ,甲基供体一方面通过肝脏BHMT酶的调控下为机体甲基化合成代谢提供甲基 ,发挥节约蛋氨酸的效应 ;另一方面通过增强机体GH和IGF -1的分泌 ,促进生长。     

半胱胺对生长期仔猪增重及有关激素的影响

经包被处理的半胱胺 (CS)分别按每周每千克体重 5 0、1 0 0、1 5 0mg剂量均匀拌入饲料中一次饲喂生长期仔猪 ,试验期为 5周。结果表明 ,三种剂量对仔猪的日增重均有提高作用 ,其中以每千克体重 1 0 0mg剂量饲喂效果最好 ,提高了 1 2 5 6% (P <0 0 5 ) ;三种剂量均可提高血清GH、T3 水平、降低SS水平 ,其中以每千克体重 1 0 0mg剂量效果最明显 ,GH、T3 水平分别提高了 1 3 6 95 % (P <0 0 1 )、2 6 5 3 % (P <0 0 5 ) ,SS水平降低了 5 6 4 5 % (P <0 0 1 )。说明CS有提高仔猪日增重的效果 ,其作用是通过降低SS水平 ,提高GH、T3 水平来实现的     

家兔肌肉组织表达外源性生长激素释放因子对增重的影响

用 Wicks法提取 pc DNA- GRF(1～ 32 )质粒并用 DNA Cacu L ator定量 ,同时制备原生质体。将 5 4只家兔随机分为 9组 ,分别注射质粒 pc DNA- GRF(1～ 32 ) 0 .4(用布比卡因处理 )、0 .4(含 10 %甘油 )、0 .4(含 2 5 %蔗糖 )、0 .2、0 .4、0 .6、0 .8mg及空质粒 0 .1mg和原生质体 1m L( ～ 组 )。分别于注射后 0、3、8、14、19、2 4、31d称重 ;于注射后 0、3、8、19、31d采血并分离血清 ,测定血清中 GH浓度 ,用 t检验进行统计学分析。结果表明 ,布比卡因处理组注射后 3d内GH浓度升高 6 5 %(P<0 .0 1) ,平均日增重 2 9g(P<0 .0 5 ) ;注射 0 .8mg组 31d平均日增重较对照组多 34 .6 2 %;注射原生质体组 31d平均日增重较对照组多 40 .5 7%(P<0 .0 5 )。 31d后将家兔屠宰 ,取注射部位肌肉提取 DNA和总RNA,用 PCR和 RT- PCR方法分别检测到了 GRF在肌肉中的存在和表达 ;用 EL ISA方法没能检测到血清中的 GRF抗体。     

甜菜碱提高蛋鸡产蛋性能的机理研究

15 36只 30 0日龄罗曼蛋鸡 ,随机分为 4组 ,分别饲喂添加 0 ,5 0 0 ,10 0 0 ,15 0 0mg/kg甜菜碱的玉米—豆粕型日粮 ,研究甜菜碱提高蛋鸡产蛋性能的作用机制。结果表明 ,日粮中添加 10 0 0mg/kg甜菜碱对提高蛋鸡产蛋性能效果最佳 ,显著提高产蛋率 10 .11% (P <0 .0 5 ) ,提高饲料转化率 6 .4 1% (P <0 .0 5 )。进一步研究发现 ,日粮中添加 10 0 0mg/kg甜菜碱使血清促卵泡生成素 (FSH)、促黄体生成素 (LH)、极低密度脂蛋白胆固醇含量和脂肪酶活性分别升高了 5 6 .4 9%(P <0 .0 1)、86 .0 3% (P <0 .0 5 )、4 3.0 7% (P <0 .0 5 )和 30 .0 7% (P <0 .0 5 ) ,血清甘油三酯和尿酸含量分别下降了 2 2 .18%(P <0 .0 5 )、18.6 6 % (P <0 .0 5 )。上述结果提示 :甜菜碱通过增强腺垂体FSH和LH的分泌 ,同时促进卵蛋白和卵脂肪沉积 ,从而提高蛋鸡产蛋率和饲料利用率     

Sex steroid hormones do not enhance the direct stimulatory effect of kisspetin‐10 on the secretion of growth hormone from bovine anterior pituitary cells

The aims of the present study were to clarify the effect of kisspeptin10 (Kp10) on the secretion of growth hormone (GH) from bovine anterior pituitary (AP) cells, and evaluate the ability of sex steroid hormones to enhance the sensitivity of somatotrophic cells to Kp10. AP cells prepared from 8–11‐month‐old castrated calves were incubated for 12 h with estradiol (E₂, 10^?8 mol/L),progesterone (P₄, 10^?8 mol/L), testosterone (T, 10^?8 mol/L), or vehicle only (control), and then for 2 h with Kp10. The amount of GH released in the medium was measured by a time‐resolved fluoroimmunoassay. Kp10 (10^?6 or 10^?5 mol/L) significantly stimulated the secretion of GH from the AP cells regardless of steroid treatments (P < 0.05), and E₂, P₄, and T had no effect on this response. The GH‐releasing response to growth hormone‐releasing hormone (GHRH, 10^?8 mol/L) was significantly greater than that to Kp10 (P < 0.05). The present results suggest that Kp10 directly stimulates the release of GH from somatotrophic cells and sex steroid hormones do not enhance the sensitivity of these cells to Kp10. Furthermore, they suggest that the GH‐releasing effect of Kp10 is less potent than that of GHRH.     

Neuropeptide Y modulates growth hormone but not luteinizing hormone secretion from prepuberal gilt anterior pituitary cells in culture

Pituitary cells, from seven 160- to 170-day-old pigs, were studied in primary culture to determine the affects NPY on LH and GH secretion at the level of the pituitary. On day 4 of culture, medium was discarded, plates were rinsed twice with serum-free medium and cells were cultured in 1 ml fresh medium without serum and challenged individually with 10(-10), 10(-8) or 10(-6) M [Ala(15)]-h growth hormone-releasing factor-(1-29)NH(2) (GRF); 10(-9), 10(-8) or 10(-7) M GnRH or 10(-9), 10(-8), 10(-7) or 10(-6) M NPY individually or in combinations with 10(-9) or 10(-8) M GnRH or 10(-8) or 10(-6)M GRF. Cells were exposed to treatment for 4 h at which time medium was harvested and quantified for LH and GH. Basal LH secretion (control; n = 7 pituitaries) was 12 +/- 6 ng/well. Relative to control at 4 h, 10(-9), 10(-8) and 10(-7) M GnRH increased (P < 0.01) LH secretion by 169, 176 and 197%, respectively. Neuropeptide-Y did not alter (P > 0.4) basal LH secretion nor 10(-8) M GnRH-induced increase in LH secretion but 10(-9) M GnRH-stimulated LH secretion was reduced by NPY and was not different from control or GnRH alone. Basal GH secretion (control; n = 7 pituitaries) was 56 +/- 12 ng/well. Relative to control at 4 h, 10(-10), 10(-8) and 10(-6) M GRF increased GH secretion by 111%, 125% (P < 0.01) and 150% (P < 0.01), respectively. Only 10(-6) M (134%) and 10(-7) M (125%) NPY increased (P < 0.04) basal GH secretion. Addition of 10(-9), 10(-8) and 10(-7) M NPY in combination with 10(-8) M GRF suppressed (P < 0.04) GRF-stimulated GH secretion. However, 10(-9) M NPY enhanced (P < 0.06) the GH response to 10(-6) M GRF. These results demonstrate that NPY may directly modulate GH secretion at the level of the pituitary gland.     

全反式维甲酸对大鼠前体脂肪细胞增殖与分化的影响

利用MTT比色检测不同浓度的全反式维甲酸(all trans Retinoic Acid ATRA)对大鼠前体脂肪细胞增殖的影响,采用油红O染色和吖啶橙染色观察大鼠前体脂肪细胞分化的形态变化;采用油红O染色提取法分析不同浓度ATRA对大鼠前体脂肪细胞分化的影响。MTT比色结果显示,低浓度(10-8～10-7mol/L)的ATRA对前体脂肪细胞的增殖具有促进作用(P<0101),而高浓度(10-6～10-4mol/L)的ATRA则抑制前体脂肪细胞的增殖(P<0101)。油红O染色和吖啶橙染色结果表明,前体脂肪细胞分化成脂肪细胞后,细胞内充满脂滴,由梭形变成椭圆形;油红O染色提取法结果显示高浓度(10-6～10-4mol/L)的ATRA能够显著抑制前体脂肪细胞的分化(P<0101),而低浓度(10-8～10-7mol/L)的ATAR却显著促进前体脂肪细胞的分化(P<0101)。     

Effects of leptin and leptin peptide amide on the release of luteinizing hormone, growth hormone and prolactin from cultured porcine anterior pituitary cells

The present study was carried out to determine whether leptin or leptin (116–130) peptide amide (lep (116–130)), an active fragment of the native protein in rats, is able to stimulate the release of luteinizing hormone (LH), growth hormone (GH) or prolactin (PRL) from cultured porcine anterior pituitary (AP) cells in vitro. The AP cells were obtained from 6 month‐old pigs and were incubated for 3 h with 10^?11?10^?7 mol/L leptin or lep (116–130) after being cultured in Dulbecco's modified Eagle's medium for 3–4 days. Leptin significantly increased the concentration of LH and GH in the culture medium at concentrations of 10^?8 and 10^?7 mol/L, respectively, compared with the controls (P < 0.05). Leptin did not increase the concentration of PRL in the culture medium. In contrast to these results, no effects of lep (116–130) on the release of LH, GH or PRL were seen in the cultured cells. These results suggest that leptin stimulates the release of LH and GH by acting directly on porcine AP cells, and that a fragment of leptin protein comprising amino acids 116–130 is not associated with the secretion of hormones in pigs.     

Effects of leptin on the release of luteinizing hormone, growth hormone and prolactin from cultured bovine anterior pituitary cells

The effects of leptin on the release of luteinizing hormone (LH), growth hormone (GH) and prolactin (PRL) were studied in cultured bovine anterior pituitary (AP) cells in vitro. The AP cells were obtained from fully‐fed Japanese Black steers and were incubated for 3 h with 10^?13 to 10^?7 mol/L of leptin after incubating in Dulbecco's modified Eagle's Medium for 3 days. Leptin significantly increased the concentration of LH in the culture medium by 45 and 44% at doses of 10^?8 and 10^?7 mol/L, respectively, compared with the controls (P < 0.05). Leptin significantly increased the concentration of GH in the culture medium by 14 and 12% at doses of 10^?8 and 10^?7 mol/L, respectively (P < 0.05). Leptin also significantly increased the concentration of PRL in the culture medium by 26% compared with the controls at a dose of 10^?7 mol/L (P < 0.05). These results show that leptin stimulates the release of LH, GH and PRL by acting directly on bovine AP cells from fully‐fed steers.     

白藜芦醇对体外培养的水牛卵丘细胞增殖活力及其相关基因mRNA表达的影响

【目的】研究白藜芦醇(resveratrol, RES)对水牛卵丘细胞体外培养过程中细胞增殖活力、激素分泌、卵丘扩展及抗凋亡和抗氧化能力的影响。【方法】用不同浓度(0(对照组)、1、10、20、30、40、50和60μmol/L)RES培养卵丘细胞,用CCK-8试剂盒测定细胞增殖活力,筛选最佳RES处理浓度及时间用于后续试验。用ELISA法测定培养液中卵丘细胞分泌的雌二醇(estradiol, E₂)和孕酮(progesterone, P₄)的含量,实时荧光定量PCR法测定卵丘细胞扩展、凋亡和抗氧化相关基因的相对表达量。【结果】与对照组相比,在体外培养24～36 h内,1、10和20μmol/L RES组水牛卵丘细胞的增殖活力均有升高趋势,10μmol/L RES处理36 h细胞活力最强,因此用于后续试验中细胞的处理。体外培养36 h时,10μmol/L RES组细胞增殖能力和E₂、P₄的分泌显著增加(P<0.05);10μmol/L RES组卵丘细胞扩展相关基因穿透素3(PTX3)、前列腺素...     

白藜芦醇对奶牛性控冻精品质和体外受精能力的影响

本研究旨在探究不同浓度白藜芦醇处理对奶牛性控冻精品质和体外受精能力的影响。在解冻后的奶牛性控冻精中分别添加0、10~(-3)、10~(-4)、10~(-5) mol/L的白藜芦醇,各组精子在受精液中获能孵育1.5 h后,测定精子质量和体外受精能力。结果表明:添加白藜芦醇均可有效降低性控冻精中活性氧(ROS)含量、提高顶体完整活精子比例(P0.05),其中10~(-3)、10~(-4) mol/L的白藜芦醇处理对降低ROS含量最为显著;10-4 mol/L的白藜芦醇处理可显著降低丙二醛(MDA)含量并提高性控冻精的卵裂率和囊胚率(P0.05)。综上所述,白藜芦醇作为一种外源性天然抗氧化剂,通过降低性控精液中过量的ROS水平、抑制精子脂质过氧化反应、保护顶体完整性,从而提高性控精子质量和体外受精能力。     

Recombinant Porcine Leptin Reduces Feed Intake and Stimulates Growth Hormone Secretion in Swine

Two experiments (EXP) were conducted to test the hypothesis that porcine leptin affects GH, insulin-like growth factor-I (IGF-I), insulin, thyroxine (T₄) secretion, and feed intake. In EXP I, prepuberal gilts received intracerebroventricular (ICV) leptin injections. Blood was collected every 15 min for 4 hr before and 3 hr after ICV injections of 0.9% saline (S; n = 3), 10 μg (n = 4), 50 μg (n = 4), or 100 μg (n = 4) of leptin in S. Pigs were fed each day at 0800 and 1700 hr over a 2-wk period before the EXP. On the day of the EXP, pigs were fed at 0800 hr and blood sampling started at 0900 h. After the last sample was collected, feeders were placed in all pens. Feed intake was monitored at 4, 20, and 44 hr after feed presentation. In EXP II, pituitary cells from prepuberal gilts were studied in primary culture to determine if leptin affects GH secretion at the level of the pituitary. On Day 4 of culture, 10⁵ cells/well were challenged with 10⁻¹², 10⁻¹⁰, 10⁻⁸, or 10⁻⁶ M [Ala¹⁵]-h growth hormone-releasing factor-(1-29)NH₂ (GRF), 10⁻¹⁴, 10⁻¹³, 10⁻¹², 10⁻¹¹, 10⁻¹⁰, 10⁻⁹, 10⁻⁸, 10⁻⁷, or 10⁻⁶ M leptin individually or in combinations with 10⁻⁸ and 10⁻⁶ M GRF. Secreted GH was measured at 4 hr after treatment. In EXP I, before injection, serum GH concentrations were similar. Serum GH concentrations increased (P < 0.01) after injection of 10 μg (21 ± 1 ng/ml), 50 μg (9 ± 1 ng/ml), and 100 μg (13 ± 1 ng/ml) of leptin compared with S (1 ± 2 ng/ml) treated pigs. The GH response to leptin was greater (P < 0.001) in 10 μg than 50 or 100 μg leptin-treated pigs. By 20 hr the 10, 50, and 100 μg doses of leptin reduced feed intake by 53% (P < 0.08), 76%, and 90% (P < 0.05), respectively, compared with S pigs. Serum IGF-I, insulin, T₄, glucose, and free fatty acids were unaffected by leptin treatment. In EXP II, relative to control (31 ± 2 ng/well), 10⁻¹⁰, 10⁻⁸, and 10⁻⁶ M GRF increased (P < 0.01) GH secretion by 131%, 156%, and 170%, respectively. Only 10⁻⁶ M and 10⁻⁷ M leptin increased (P < 0.01) GH secretion. Addition of 10⁻¹¹ and 10⁻⁹ M leptin in combination with 10⁻⁶ M GRF or 10⁻¹¹ M leptin in combination with 10⁻⁸ M GRF-suppressed (P < 0.05) GH secretion. These results indicate that leptin modulates GH secretion and, as shown in other species, leptin suppressed feed intake in the pig.     

花生四烯酸对日本沼虾肝胰腺细胞脂质代谢基因表达的影响

本试验旨在评价细胞培养液中花生四烯酸(arachidonic acid,ARA)浓度对日本沼虾肝胰腺细胞活力及脂质代谢相关基因表达的影响。分离日本沼虾肝胰腺细胞,使用M199完全培养液培养5 d后换成含ARA的培养液,ARA浓度分别为0(ARA1)、50(ARA2)、100(ARA3)、200(ARA4)和1 000μmol/L(ARA5),测定12和24 h时脂质代谢相关基因的表达水平,以及24h时细胞活力。结果表明:原代肝胰腺细胞使用完全培养液时,生长状况良好,能存活15 d左右;ARA5组24 h时细胞活力显著低于ARA1和ARA2组(P0.05);高浓度的ARA降低了12和24 h时Δ4脱饱和酶(Δ4 FAD)、Δ6脱饱和酶(Δ6 FAD)、碳链延长酶6(Elovl6)、B类Ⅰ型清道夫受体(SR-BⅠ)、脂肪酸结合蛋白10(FABP10)、乙酰辅酶A结合蛋白(ACBP)基因表达水平;ARA作用12 h时,ARA2组SR-BⅠ基因表达水平显著高于其余各组(P0.05),ARA2和ARA3组FABP10基因表达水平显著高于ARA1和ARA5组(P0.05),ARA3组ACBP基因表达水平显著高于其余各组(P0.05);ARA作用24 h时,ARA2组SR-BⅠ、FABP10和ACBP基因表达水平显著高于其余各组(P0.05)。由此可见,细胞培养液中ARA浓度会影响日本沼虾肝胰腺细胞活力及脂质代谢相关基因的表达,过高的ARA浓度(1 000μmol/L)会降低细胞的活力,适宜的ARA浓度(50~100μmol/L)可促进脂肪酸脱饱和酶、碳链延长酶及脂肪酸转运相关基因的表达。