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1.
甜叶菊茎尖组培苗生根及移栽的研究   总被引:1,自引:0,他引:1  
采用甜叶菊的幼嫩茎尖为外植体,MS为基本培养基。在添加6-BA1.0mg/L+蔗糖30g/L+琼脂6g/L的诱导培养基上.进行丛生芽的诱导培养。如果不感染病菌,诱导出芽率可达100%。继代增殖培养基采用MS+6-BA0.5mg/L+NAA0.05mg/L+蔗糖30g/L+琼脂6g/L,继代培养30d后,丛生芽数可增殖14--15倍。在1/4MS+IBA0.1mg/L+NAA0.1mg/L+蔗糖30g/L+琼脂6g/L+性炭1g/L的生根培养基上诱导生根,效果最好,生根率达100%.组培苗出瓶前先敞口炼苗2d.生根苗移栽后成活率可达95%以上。  相似文献   

2.
安祖花的离体培养及快速繁殖   总被引:6,自引:0,他引:6  
以安祖花(Anthurium andraeanum Lind)无菌苗叶,叶柄及茎段为外植体,在附加1mg/L的6-BA的改良MS培养基上经经光培养可诱导愈伤组织形成;在1/2MS+NAA0.1mg/L 6-BA0.5mg/L的培养基上增殖其愈伤组织及诱导丛生芽;在1/2MS NAA0.05mg/L 6-BA0.5mg/L+水解乳蛋白400mg/L上壮苗;在1/2MS+NAA0.05mg/L 400mg/L活怀炭0.4%上生根,小苗移栽成活率高且生长良。  相似文献   

3.
以绿巨人带顶芽或腋芽进行组培繁殖,研究表明:适合诱导培养基为1/2MS+6-BA3.Omg/L,诱导率为90%;适合不定芽增殖培养基为Ms+6-BA4mg/L+NAA0.5mg/L;适合分化培养基为Ms+6-BA0.5mg/L+IBA0.1mg/L;适合生根诱导培养基为1/2MS+NAA1.0mg/L或1/2MS+IBAl.0mg/L,生根率可达90%以上;继代过程中次数多了会出现玻璃化现象,而6-BA浓度4或0.5mg/L与NAA0.5mg/L组合的交替使用可有效降低玻璃化的发生,也有利于芽苗的增殖;  相似文献   

4.
白掌的离体快速繁殖初探   总被引:1,自引:0,他引:1  
以白掌带顶芽或腋芽茎段,进行组培试验,研究表明:适合诱导培养基为1/2MS+6-BA3.0mg/L,诱导率为100%;适合不定芽增殖培养基为Ms+6-BA4mg/L+NAA0.5mg/L,适合生根诱导培养基为1/2MS+NAA1.0mg/L或1/2MS+IBA1.0mg/L,生根率可达90%以上;继代过程中次数多了会出现玻璃化现象,而6-BA浓度4或0.5mg/L的交替使用有效减低玻璃化的发生,也有利于芽苗的增殖。  相似文献   

5.
象脚丝兰的组织培养及快速繁殖试验研究简报   总被引:2,自引:0,他引:2  
采用象脚丝兰的顶芽或侧芽在MS 6-BA4mg/L NAA0.1mg/L培养基上培养15d后芽开始生长,芽长2-4cm时对半纵切转入同一培养基继代增殖培养20-25d,繁殖系数3.07,待芽长3-4cm时分割成单株于1/2MS NAA1mg/L 活性炭0.3%的培养基上进行生根培养25d,生根率100%,移植成活率85%。  相似文献   

6.
银后粗肋草的离体培养和快速繁殖   总被引:1,自引:0,他引:1  
以银后粗肋草的幼嫩叶片为材料,研究不同植物生长调节剂组合对愈伤组织诱导和分化的影响;以带侧芽的茎段为材料,研究不同接种方式、不同6-BA和NAA组合对丛生芽诱导的影响;以愈伤组织诱导的不定芽和茎段诱导的丛生芽为材料,研究不同植物生长调节剂组合对丛生芽的增殖和生根的影响;最后研究不同移栽基质组合对试管苗移栽成活的影响。结果表明:(1)叶片接种在MS+6-BA 2.5 mg/L+NAA 0.1 mg/L培养基培养中60 d后,愈伤组织诱导率为63.41%,不定芽再生率为26.88%,平均生成芽数为3.80个。(2)带侧芽的茎段水平放置接种在MS+6-BA 2.0 mg/L+NAA 0.1 mg/L培养基上,丛生芽诱导率为91.91%,平均生成芽数为5.29个。(3)将经叶片诱导愈伤组织分化所得的不定芽和经茎段诱导所得的丛生芽切成单株,接种到MS+6-BA 3.0 mg/L+NAA 0.1 mg/L或MS+KT 3.0~4.0 mg/L+NAA 0.1 mg/L增殖培养基中,不定芽和丛生芽增殖和生长良好。(4)将在增殖培养基中长至4 cm以上的小植株转入到1/2 MS+NAA 0.2 mg/L的生根培养基中培养20 d后,生根率达100%,平均根长4.49 cm,每株平均根数6.80条。(5)生根苗移栽到1/2木屑+1/4珍珠岩+1/4菜园土混合基质中,成活率达95.40%。  相似文献   

7.
参考幼叶、子叶为外植体诱导丛生芽的方法,利用花生种子胚生长旺盛的特性,以花生种子胚中段为外植体材料建立一个新植株再生体系。结果表明,在含3.0mg/L的6-BA的MS培养基上,培养30d可以诱导出丛生芽,诱导率达到92.5%;丛生芽转至1/2MS+0.2mg/LIBA+0.1mg/LNAA的培养基中培养2~3周,生根形成完整植株。  相似文献   

8.
粉蕉组织培养与快速繁殖技术研究   总被引:1,自引:0,他引:1  
以粉蕉的全顶芽块为外植体,进行多方法无菌外植体建立试验,利用正交试验设计方法[L9(3^4)]研究了增殖培养基中6-BA、KT、NAA和KH2PO4等四种因素对芽的总增殖率和大中芽率的影响。试验结果表明:无菌外植体建立时,升汞消毒时间在20-30min,且进行分次消毒外建成功率较高;KH2PO4是影响芽增殖结果的主要因素,MS(内KH2PO4 150%) 6-BA4mg/L NAA0.1mg/L或MS(内KH2PO4 200%) 6-BA3mg/L NAA0.05 mg/L两种培养基是可适用的芽增殖培养基.而在MS KT0.2mg/L NAA1mg/L IBA2mg/L AC3g/L培养基中能诱导丛生芽生根形成小植株。  相似文献   

9.
以黑绒观音莲小球茎顶端切段为外植体,进行组织培养与快繁试验。结果显示,在MS培养基中加入较高浓度的6-BA对顶芽和丛生芽的诱导及继代增殖有利:生根培养基可用1/2MS+6-BA0.3-0.5mg/L NAA0.8-1.0mg/L,生根率达100%。生根苗移栽到混合30%-40%珍珠岩的椰糠中,成活率达95%以上。采用自然变化温光条件下培养有利于瓶苗的生根和生长,提高瓶苗素质及移栽成活率。  相似文献   

10.
H.11648麻珠芽组织培养技术研究   总被引:3,自引:0,他引:3  
用H.11648麻珠芽为材料进行组织培养,结果表明:在MS+6-BA5mg/L+NAA0.3mg/L+白糖3%+琼脂0.65%培养基中,诱导产生不定芽,其诱导率为72%,不定芽在MS+6-BA3mg/L+NAA0.1mg/L+白糖5%+琼脂0.8%培养基中,诱导产生丛芽,其诱导倍数是1.57,对丛芽进行继代培养、实现丛芽平均每代的增殖率228%。用MS+NAA1.0mg/L+IBA0.3mg/L+活性炭0.2%+白糖3%培养基诱导生根,其生根率为82%;将生根苗移至菇渣5:塘泥3:表土1:河沙1配方基质的苗床假植,移栽平均成活率为84%。该项技术的研究成功,为今后剑麻种苗的工厂化生产提供了技术支撑。也为开展剑麻的生物工程育种提供了技术基础。  相似文献   

11.
In the western Sahel, indigenous plants become important staples when cereal harvests are inadequate to support populations inhabiting that region of Africa. The purpose of this study was to assess the nutrient content of several of these edible wild plants. The leaves of the following seven plant foods were analyzed: Ziziphus mauritiana, Cerathotheca sesamoides, Moringa oleifera, Leptadenia hastata, Hibiscus sabdarifa, Amaranthus viridi, and Adansonia digitata. The fatty acid, vitamin E, carotenoid, selected mineral and amino acid contents of these plant foods were determined. These same analyses were performed on the fruit of the Adansonia digitata. In quantitative and qualitative terms, Amaranthus viridis was found to be an excellent source of protein. Its amino acid composition compared favorably to that of a World Health Organization (WHO) protein standard. It also contained considerable amounts of the two fatty acids that are essential in humans (linoleic and -linolenic) and a number of minerals including iron, magnesium, calcium and zinc. The leaves of Hibiscus sabdarifa contained an appreciable quantity of protein the composition of which was comparable to the WHO standard. The mineral content of the leaves of this plant was also exceptionally high; noteworthy was its high zinc content. H. sabdarifa also contained significant quantities of the two essential fatty acids. Ziziphus mauritiana was an excellent source of the essential fatty acid linoleic acid and several of the metals including iron, calcium, magnesium and zinc. Its content of other essential nutrients, however, was rather low. In general, Adansonia digitata leaves were nutritionally superior to the fruit of the tree; however, the fruit did contain useful quantities of potassium, phosphorus, zinc and -linolenic acid. The Leptadenia hastata leaves were an especially good source of lutein and -carotene. These data should be useful to the people who inhabit the western Sahel in helping them devise healthy diets during times when cereal staples are in short supply.  相似文献   

12.
通过电解质外渗法和匍匐茎恢复试验对‘阳江’狗牙根及其12个通过形态鉴定选出的坪用价值高且花序密度低的诱变后代进行抗寒性鉴定。电解质外渗法结果表明:诱变后代间的抗寒性具有较大差异,其叶片半致死温度(LT50)的变异范围为-7.6~-0.2℃(最低值与最大值相差7.4℃);参试材料抗寒性由强到弱依次为M18>M4>M26>M28> M22>阳江>M29>M31>M10>M37>M16>M1>M25,其中,有5个诱变后代抗寒性优于亲本,分别是M18、M4、M26、M28、M22。匍匐茎恢复实验结果表明:诱变后代M1、M22、M26、M31、M25在0℃和-5℃低温胁迫后的恢复生长率都高于亲本,恢复能力均优于亲本;M10、M37、M28在-5℃低温胁迫下,恢复生长能力低于亲本,抗寒性相对较弱,M16和M4在0℃和-5℃低温胁迫下,恢复生长率都低于亲本,抗寒性明显弱于亲本。综合2种方法鉴定结果显示:诱变后代M1、M25的恢复能力较强;M4、M28的叶片抗寒性较好,青绿期较长;M22、M18、M26的叶片抗寒性和匍匐茎恢复能力均较强;M29,M31的叶片抗寒性和匍匐茎恢复能力与亲本相似;M10、M16、M37的叶片抗寒性和匍匐茎恢复能力均较弱,整体抗寒性较弱。  相似文献   

13.
对造成福建省漳州市东山县的芦笋枯萎病病原菌进行分子鉴定,以期确定病原菌的属、种名,为防治该病害寻求理论依据。试验对分离纯化的病原菌核糖体DNA的ITS区进行测序,在Genbank中搜索其同源性并构建它们的系统发育树,结果表明:引起芦笋枯萎病的病原菌是镰刀属中的尖孢镰刀菌(Fusarium oxysporum),且病原菌之间的同源性高达89%,遗传差异不显著。  相似文献   

14.
One hundred and fifty-six pre-roost gatherings of starlings were observed at 28 sites around a roost in west Norfolk during winter. Pre-roost gatherings occurred more frequently at individual farmyards compared with individual fields, but 82% of the sites where gatherings occurred were on fields. The size of gatherings was greater, the closer to the roost. Birds in pre-roost gatherings on fields of autumn-sown winter wheat spent most of the time feeding and had a diet composed almost exclusively of wheat seeds. Birds in gatherings on grass fields also fed intensively whereas only 31% of birds at farmyards were feeding. The implications that these results have with regard to potential damage to fields of winter wheat and around farmyards are discussed.  相似文献   

15.
橡胶树丛枝病病原的抗血清制备与应用   总被引:5,自引:1,他引:4  
以感染橡胶树丛枝病病原的长春花为材料,制备得到橡胶丛枝病病原菌抽提液,以抽提液为抗原免疫家兔,制备抗血清,经微量沉淀测定,抗血清效价为1:2048。应用橡胶丛枝病抗血清检测橡胶褐皮病,无症苗木检出率达30%-37%,可疑的褐皮病树检出率达85.7%。  相似文献   

16.
为了寻找一种高效的荔枝果实瞬时基因表达方法,本研究以荔枝品种‘新球蜜荔’(Litchi chinensis Sonn. var. ‘Xinqiumili’)为试材,利用农杆菌注射法对荔枝果实组织进行转化,研究了果实发育时期、菌株种类、注射部位、取样时间、菌液浓度等对转化效率的影响。结果表明:选择果肉已完全包裹种子的Ⅱb期果实进行连体注射,在果柄、果皮、种子、果肉分别注入OD600值为2.4的农杆菌菌株GV3101,4 d后取样进行检测,4个组织的GUS染色率较高。本研究成功建立了适用于荔枝果实的基因瞬时表达系统,为今后快速鉴定荔枝果实相关基因功能提供了基础。  相似文献   

17.
2020年10月17日,《中华人民共和国生物安全法》(以下简称《生物安全法》)通过全国人大常委会的审议,自2021年4月15日起施行.生物技术的研究与应用安全是《生物安全法》涉及的主要内容之一,基因编辑作为近几年生物技术领域的研究热点,其安全性评价和监管备受关注.本文概述了基因编辑技术的应用现状,比较了不同国家对基因编...  相似文献   

18.
为深入研究昆虫病原真菌蝉拟青霉疏水蛋白PChyd基因的功能,根据蝉拟青霉基因组信息克隆疏水蛋白PChyd基因,对该基因序列进行生物信息学分析,使用qRT-PCR技术对其在不同培养条件或阶段下的表达模式进行分析,并通过酶切酶连的方法构建了该基因的敲除载体。结果表明:PChyd基因的开放阅读框序列全长303 bp,编码100 aa,包含22 aa的信号肽序列和70 aa疏水蛋白功能区域。系统发育分析显示该基因与粗糙虫草菌亲缘关系最近。qRT-PCR结果显示PChyd基因在PDA培养的菌丝体、诱导的附着胞、诱导的芽生孢子中表达量显著高于另外2个样品,其中芽生孢子表达量最高,暗示该基因在蝉拟青霉侵染初期和在昆虫血腔中定殖阶段可能具有重要作用。凝胶电泳结果表明,成功构建了该基因的敲除载体,扩增出含有上臂、HPH、下臂的3356 bp左右的片段。本研究为进一步探究蝉拟青霉疏水蛋白PChyd基因的致病机理、生防工程菌的改造奠定了基础。  相似文献   

19.
波罗蜜叶片突变体叶绿素含量测定和超微结构观察   总被引:1,自引:0,他引:1  
波罗蜜(Artocarpus heterophyllus)是一种热带果树,至今对其突变体的研究少有报道。本研究以波罗蜜叶片为试验材料,探究其叶片出现白化和返绿现象的可能原因。(1)用分光光度计法、比色法测定波罗蜜叶片突变体和正常绿叶的叶绿素及叶绿素前体物质含量;(2)用叶绿素酶Elisa试剂盒测定叶绿素酶活性;(3)用透射电子显微镜对叶绿体的超微形态结构进行观察。研究结果表明:(1)不同叶色所产生的叶绿素a含量、叶绿素b含量、叶绿素总含量、类胡萝卜素含量均存在显著差异;(2)波罗蜜叶片突变体和正常绿叶的叶绿素合成前体物质含量之间并没有出现显著性差异;(3)对波罗蜜叶片的叶绿素酶活性进行测定时,发现其活性出现显著差异,但对其叶绿素含量的差异性并没有产生较大影响;(4)观察波罗蜜叶片内叶绿体超微形态结构时,发现正常绿叶的叶绿体形态完好且数量较多,白化叶和返绿叶的叶绿体内部结构存在缺陷,其原因是叶绿体基粒构建阶段受阻;基于测定波罗蜜叶片中的叶绿素、叶绿素前体物质含量和叶绿素酶活性,并观察波罗蜜叶片内叶绿体的超微形态结构,对得到的数据结果进行比较分析。本研究推测是在叶绿素合成阶段,原脱植基叶绿素合成叶绿素时受阻及叶绿体发不良导致波罗蜜出现白化和返绿现象,为今后进一步综合研究波罗蜜突变体提供理论依据。  相似文献   

20.
Mechanism of action of insecticidal secondary metabolites of plant origin   总被引:1,自引:0,他引:1  
Insect pest management is facing the economic and ecological challenge worldwide due to the human and environmental hazards caused by majority of the synthetic pesticide chemicals. Identification of novel effective insecticidal compounds is essential to combat increasing resistance rates. Botanicals containing active insecticidal phytochemicals appear to be promising to address some of these problems. Therefore, there is a continuous need to explore new active molecules with different mechanisms of action. Secondary metabolites present in plants apparently function as defense (toxic), which inhibits reproduction and other processes. The phytochemical biomolecules could be used for maximizing the effectiveness and specificity in future insecticide design with specific or multiple target sites, while ensuring the economic and ecological sustainability. In this article, the current state of knowledge on phytochemical sources and insecticidal activity, their mechanism of action in insects, resistance, and promising advances made in phytochemical research are reviewed.  相似文献   

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