共查询到19条相似文献,搜索用时 468 毫秒
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茄子青枯病抗性基因的遗传分析及其AFLP标记 总被引:8,自引:0,他引:8
以茄子高感青枯病品种‘三月茄’与高抗品种‘S69’的F1、F2为材料, 对青枯病抗性遗传进行了分析, 同时开展了与抗性基因连锁的AFLP标记鉴定。结果表明: ‘S69’对青枯病的抗病性属于不完全隐性遗传, 感病性属于不完全显性; 抗性由1~2对基因控制。对86个F2单株AFLP分析, 鉴定出1个与‘S69’抗性基因连锁的AFLP标记39A980 (该引物组合为E-ACC /M-CTG) , 该标记与抗性基因间的交换值为4.65% , 遗传距离为4.9 cM。 相似文献
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SSR标记技术在甜瓜杂交种纯度检验中的应用 总被引:11,自引:0,他引:11
以两个甜瓜杂交品种(系) ‘东方蜜1号’和‘01231’及其亲本为材料, 用SSR分子标记技术研究杂种与其双亲之间扩增谱带的多态性, 以甄别真假杂种。所试验的23对SSR引物中有8对引物分别在两个甜瓜杂交种和其双亲之间存在扩增多态性, 表现为: 多数SSR引物对自交系的扩增只出现1条带,但部分引物在某些自交系中扩增出2条带, 杂交种条带均为父母本的互补型, 很适合做杂交种纯度鉴定。用引物M6 对‘东方蜜1号’和引物M17对‘01231’各100粒单种子进行SSR鉴定, 所测纯度分别为100%和96% , 与田间纯度99.6%和96.2%非常接近, 显示出SSR标记技术在甜瓜杂交种子纯度的室内快速检测中有广泛应用前景。 相似文献
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Veerendra K. Verma T.K. Behera A.D. Munshi Swarup K. Parida T. Mohapatra 《Scientia Horticulturae》2007
Ten inbred lines of ash gourd [Benincasa hispida (Thunb.) Cogn.] were crossed to produce 45 F1 hybrids (without reciprocal) which were evaluated along with the parents for 20 growth- and yield-related traits, in a replicated field trial. High level of heterosis was observed among the hybrids for most of the traits examined, including yield. These inbred lines were analysed by using 42 RAPD primers those produced 282 DNA marker bands. A total of 130 RAPD markers were obtained with a mean of 3.1 per primer, which in combination discriminated all the inbreds from each other. Pair-wise genetic distance measurements ranged from 0.07 to 0.31, suggesting a wide genetic diversity for these inbreds. These inberds were also analysed with five ISSR primers of which four were informative. Twenty-six ISSR marker bands were generated of which 11 were polymorphic with an average of 2.80 per primers. The percentage of polymorphic bands produced were higher in ISSR markers (>80%) than generated through RAPD markers (46%). Although the results indicated significant positive correlations of genetic distance with hybrid performance and heterosis, the RAPD based genetic distance measures and use of limited ISSR markers in this present study could not effectively predict hybrid performance in this crop. The genetic variation among ash gourd inbred lines examined, herein, defined a marker array (combined ISSR and RAPD) for the development of a standard reference for further genetic analyses, and the selection of potential parents for predicting hybrid performance and heterosis. 相似文献
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为探索基于SNP标记的分子遗传距离预测辣椒产量杂种优势的可能,实现辣椒杂种优势早期预测并筛选强优势组合,以12份辣椒自交系为亲本,采用个别分析和分组分析两种方法,开展了全基因组SNP标记为基础的分子遗传距离与产量杂种优势关系的比较。在个别分析中,从12份自交系中随机选取8份自交系,发现遗传距离与产量杂种优势之间存在不规则变化,有62.5%符合遗传距离越远杂种优势越强的规律;在分组分析中,发现随着遗传距离增大,杂种优势值总体呈现上升趋势。说明辣椒亲本间遗传距离越大,获得高产强优势组合的可能性越大,基于SNP标记的遗传距离可用来预测其后代杂种优势。 相似文献
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辣椒抗根结线虫基因Me3的精细定位 总被引:1,自引:0,他引:1
以含Me3基因的抗根结线虫辣椒自交系‘HDA149’为父本,感根结线虫辣椒自交系‘8214’为母本,构建了一个2 133株的F2作图群体。采用群体分离分析法(Bulked Segregant Analysis,BSA),构建抗、感病两个DNA池,对由两亲本筛选出来的285对引物进行扩增分析,发现引物SSCP_B322和EPMS658在抗、感池间具有稳定的多态性。继续用这两对引物对F2单株进行扩增,以JoinMap 3.0软件分析发现SSCP_B322和EPMS658标记位于Me3基因两侧,遗传图距分别为0.56 cM和1.33 cM。运用回交群体BC1和F3群体验证实了这两个标记,可有效用于辣椒抗线虫分子标记辅助育种,也为图位克隆Me3基因奠定了基础。 相似文献
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利用重组自交系群体构建番茄AFLP 遗传连锁图谱 总被引:1,自引:0,他引:1
以普通栽培番茄(Solanum lycopersicum)99165-30为母本,野生多毛番茄(Solanum habrochaites)LA1777为父本进行杂交,通过单粒传得到了含有80个F5︰6家系的重组自交系分离群体,利用荧光AFLP分子标记技术构建番茄分子遗传连锁图谱。AFLP标记采用MseⅠ和EcoRⅠ两种内切酶及荧光标记(IRD-700或IRD-800)的E + 3和非荧光标记的M + 3引物组合进行选择性扩增,扩增结果经95 ℃预变性后在6%变性聚丙烯酰胺凝胶上电泳2.5 h,运用LICOR 公司的NEN Global Edition IR2 DNA Analyzer(Model 5200 LI-COR Biosciences,Lincoln,NE)荧光扫描检测DNA多态性。对RILs群体中产生分离的274个AFLP标记运用JoinMap 3.0软件分析,得到一张番茄分子遗传连锁图谱,图谱总长度为662 cM,共包括18个主要连锁群,125个多态性分子标记。每条连锁群上的标记数在3 ~ 22个之间,连锁群的长度在14.0 ~ 58.0 cM的范围内,平均图距在 2.27 ~ 13.3 cM。总平均距离5.3 cM,本研究中构建的番茄永久遗传图谱,为番茄分子辅助育种及重要农艺性状的定位奠定了基础。 相似文献
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M. Sangeeta Kutty Lalitha Anand 《The Journal of Horticultural Science and Biotechnology》2013,88(4):774-778
SummaryRandomly amplified polymorphic DNA (RAPD) markers were used to estimate genetic diversity among 24 cultivars of short-day onions. Total genomic DNA was extracted and subjected to RAPD analysis using 90 arbitrary 10-mer primers. Of these, 15 primers were selected which yielded 137 bands, 91.24% of which were polymorphic. None of the primers produced a unique banding pattern for each cultivar. RAPD data were used to calculate a Squared-Euclidian Distance matrix which revealed a minimum genetic distance between cultivars ‘AFLR-722’ and ‘PBR-140’, and a maximum genetic distance between cultivars ‘PBR-139’ and ‘A.Kalyan’, and ‘MS-48’ and ‘A.Kalyan’. Based on the distance matrix, cluster analysis was done using a minimum variance algorithm.The dendrogram thus generated, based on Ward’s method, grouped the 24 onion cultivars into two major clusters. The first cluster consisted of cultivars from the northern region, and the second of cultivars from the southern region of India. The present study shows that there is high diversity among the onion cultivars selected and indicates the potential of RAPD markers for identification and maintenance of onion germplasm for crop improvement purposes. 相似文献
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为筛选出一套大白菜(Brassica rapa L. ssp. pekinensis)简单重复序列(Simple sequence repeat,SSR)的核心引物,以54份大白菜骨干自交系为材料,从已发表的2 129对白菜SSR引物中,初步筛选出分布于白菜整个基因组、具有唯一扩增位点、扩增稳定性及重复性好的多态性SSR引物51对。进一步根据引物的多态性信息量(PIC)、等位基因数量、位点的物理位置和主成分分析(PCA)等分析结果,确定了一套包含28对SSR引物的核心引物组合。比较核心引物组合、51对多态性SSR引物和123个标记(72个SNP标记与51对多态性SSR引物)对54份大白菜骨干自交系的聚类分析结果,三者具有较高的一致性;该套引物可将262份大白菜高代自交系区分开,具有较好的鉴别能力。利用这套核心引物构建了242份大白菜品种的指纹图谱。该研究获得的28对SSR引物可以用于大白菜的遗传多样性分析和品种核酸指纹库构建等研究,可为大白菜新品种的特异性、一致性、稳定性检测体系的建立和新品种保护提供技术支持。 相似文献
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SRAP分子标记预测萝卜遗传距离与杂种优势的相关性 总被引:1,自引:0,他引:1
探讨应用SRAP分子标记分析萝卜亲本遗传距离从而预测F1杂种优势的可行性,为萝卜杂交种亲本的性状改良及F1杂种优势选配提供参考。选择来源不同、农艺性状有差异的11个耐抽薹萝卜亲本材料,按不完全双列杂交法组配30个杂交组合,运用SRAP分子标记分析萝卜亲本遗传距离以及其和萝卜农艺性状杂种优势之间的关系。结果证明:通过运用327个多态性SRAP标记来计算11个亲本间的遗传距离,其范围在0.162~0.486,且平均为0.291。亲本间SRAP标记的遗传距离与F_1杂种表现既有相关显著,也有相关不显著;与各农艺性状杂种优势相关达到极显著、显著相关或不显著相关水平,表明SRAP分子标记遗传距离在一定程度上能反映出农艺性状杂种优势的强弱,但其相关程度还不能准确地预测杂种优势。 相似文献
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甜瓜抗蔓枯病基因Gsb-4 的分子标记 总被引:1,自引:0,他引:1
以甜瓜(Cucumis melo L.)抗蔓枯病自交系PI482398(含抗蔓枯病基因Gsb-4)和感病自交系‘白皮脆’以及它们的F1、BC1P1、BC1P2、F2 群体为材料,苗期进行蔓枯病菌(Didymella bryoniae)接种鉴定,结果表明甜瓜抗蔓枯病基因Gsb-4 为单显性遗传。利用集团分离分析法(bulked segregant analysis,BSA)对89 对SSR 引物进行筛选,引物CMTA170a 在抗性材料中可扩增出约为120 bp 的条带,并与抗性基因Gsb-4 表现出连锁关系。统计了CMTA170a 在118 个F2 单株上的多态性,并利用MAPMAKER/Exp version 3.0b 软件进行了计算,其与Gsb-4 的遗传连锁距离为5.14 cM。 相似文献