Expression of Endothelin‐1 and Endothelin Receptor A in Canine Ovarian Tumours

Ovarian tumours have a low incidence in bitch. Endothelin (ET‐1) and endothelin A receptor (ET‐A) are overexpressed in human ovarian cancer. Twenty canine ovarian tumours and five normal samples were first evaluated by western blotting and then immunohistochemically for ET‐1 and ET‐A expression. Seventeen out of twenty tumours were ET‐1 positive. Eight out of twenty tumours were ET‐A immunohistochemically positive. At molecular level both proteins were proven to be expressed in normal as well as in tumour samples. Our results show that ET‐1 and ET‐A are overexpressed in canine ovarian tumours, suggesting a potential role of these two molecules in canine ovarian carcinogenesis.     

犬乳腺肿瘤的病理组织学观察及p63蛋白的表达

通过对35例犬乳腺肿瘤的临床病史调查、光学显微镜观察和免疫组化染色,探讨了犬乳腺肿瘤的临床病理形态学特点、诊断和鉴别诊断.结果显示34例发生在5岁以上,6岁～10岁为发病高峰期;51.4％的病例为恶性肿瘤,其中44.4％的乳腺癌发生转移.免疫组化染色结果显示,在正常乳腺和良性肿瘤组织中,p63蛋白100％表达,并且仅仅...     

Conditions of the Canine Oesophagus*—III Anaesthesia for Oesophageal Surgery

Abstract— Anaesthesia for oesophageal surgery presents very few peculiar features, and any sound anaesthetic technique will provide satisfactory results. It is considered that relaxant drugs have a definite place in anaesthesia for this type of surgery.
Résumé— L'anesthésie pour la chirurgie oesophagiennc présente peu de particularity spéciales et une technique anesthésique parfaitedonn erait des réultats satisfaisants. II est généralement remarqué que les drogues relaxantes ont une place marquée dans l'anesthéie pour ce genre de chirurgie.
Zusammenfassung— Anästhesie bei Speiseröhrenoperationen ist von sehr wenigen besonderen Umständen begleitet und jede zuverlässige anästhetische Methode fiihrt zu zufriedenstellenden Resultatcn. Eine definitive Stellung wird den Entspannungsdrogen für die Anästhesie bei Operationen dieser Art eingeräumt.     

Symposium on Conditions of the Canine Oesophagus*—I Foreign Bodies in the Oesophagus

Abstract— A report is presented of observations on a series of seventy-one cases of canine oesophageal obstruction. Predisposing factors, symptomatology, diagnosis, treatment and complications are discussed.
Résumé— Un compte-rendu est présenté sur les observations d'une série de 71 cas d'obstruction oesophagienne canine. Les facteurs prédisposants, la symptomatologie, la diagnose, le traîtement et les complications sont discutés.
Zusammenfassung— Berichterstattung über Beobachtungen einer Serie von 71 Fällen von Speiseröhrenobstruktion bei Hunden. Prädispositionsfaktoren, Symptomatologie, Diagnosen, Behandlung und Komplikationen werden besprochen.     

Computerized Cytomorphometric Analysis of Nuclear Area,Nuclear Perimeter and Mean Nuclear Diameter in Spontaneous Canine Mammary Gland Tumours

Fifty-two spontaneous canine mammary gland tumours (fibroadenomas (n = 8), tubulopapillary carcinomas (n = 9), solid carcinomas (n = 6), anaplastic carcinomas (n = 7), fibrosarcomas (n = 9), liposarcomas (n = 9) and osteosarcomas (n = 4) were analysed by computer-assisted nuclear morphometry in Hemacolor-stained cytological specimens. Computerized cytomorphometry was performed and the nuclear area, nuclear perimeter and mean nuclear diameter of investigated tumours were assessed. A minimum of 100 nuclei per lesion were examined. The statistical analysis revealed statistically significant differences between benign and malignant neoplasms. The results indicated that computer-assisted nuclear morphometry could be used as an additional method for differentiation of benign from malignant canine mammary gland tumours in cytological specimens.     

Multivariate Survival Analysis of Histological Parameters and Clinical Presentation in Canine Cutaneous Mast Cell Tumours

The Patnaik histological grade is a good method for the prediction of long-term mast cell tumour behaviour but it is influenced by subjective inter-observer variations and intratumoral heterogeneity. The present study evaluated each of the histopathological parameters used to formulate Patnaik’s grade in terms of prognosis and tested whether they have a different prognostic sensitivity, thereby disclosing which could be considered more useful in the prediction of tumour recurrence and patient survival. Clinical presentation (single or multiple tumours) was also considered as possible prognostic factor. The results demonstrated that individual histological criteria together with multiple presentation may be of value in predicting the outcome of mast cell tumours. Among these, invasiveness (β1.85; standard error 1.15) and the number of mitotic figures (β3.01; standard error 1.18) showed high prognostic significance (Cox proportional hazard regression for censored data ; chi-squared = 15.52, degree of freedom = 6, p = 0.016) and could serve as reliable prognostic indicators avoiding more subjective parameters such as cellular differentiation, nuclear morphology and tumoural pattern. R. Preziosi Deceased     

Serum and Tissue Steroid Hormone Levels in Canine Mammary Tumours: Clinical and Prognostic Implications

Hormonal dependency of canine mammary tumours (CMT) has been studied over the last few decades. However, studies assessing the prognostic and predictive potential of serum and/or tissue steroid hormone levels are still scarce in CMT. To the best of our knowledge, this is the first report relating serum and tissue levels of steroid hormones and prognosis in dogs. Serum and tumour tissue from 45 female dogs with spontaneous CMT were included in the study. Moreover, serum and normal mammary tissue from 13 healthy female dogs were also included as controls. Steroid hormones were determined by competitive enzyme immunoassay. Overall, levels of steroid hormones in serum and tissue homogenates were significantly different between malignant and benign mammary tumours (p < 0.01), except for progesterone (P4) serum levels that revealed no statistical differences between groups. In malignant tumours, oestrone sulphate (SO4E1), dehydroepiandrosterone (DHEA), androstenedione (A4), testosterone (T) and P4 elevated tissue concentrations were significantly associated with tumour relapse and/or distant metastasis during follow‐up. A significant association was found between elevated tissue SO4E1 (p = 0.003), 17β‐oestradiol (E2) (p = 0.036), DHEA (p = 0.022), A4 (p = 0.001) and P4 (p = 0.013) concentrations and shorter disease‐free survival and overall survival in female dogs with malignant mammary tumours. The high levels of tissue steroids found in cases of poor prognosis open the possibility of additional new therapeutic approaches. Future clinical trials will be needed to clarify the usefulness of targeting steroid hormones in the treatment of this neoplastic disease.     

Pathology of Bovine Laminitis Especially As Regards Vascular Lesions

Macro- and microscopical changes in the hooves of 14 cattle having a clinically established diagnosis of laminitis are described, Deviation of the pedal bone was found in 10 of the animals. In acute laminitis, the microscopical changes in the corium were hyperaemia, oedema, thrombosis and haemorrhages. Degenerative changes were found in the epidermis especially in the vicinity of thromboses of vessels in the corium. In chronic laminitis, arteriolosclerosis and arteriosclerosis were constant findings in the corium. There was also a high frequency of chronic thrombi and chronic granulation tissue. Prominent features in the lamellae epidermales were hyper- and parakeratosis. As judged from changes in arterioles and arteries, hypertension is suggested to be a pathogenetic factor in bovine laminitis.cattle; laminitis; histology; arteriolosclerosis; arteriosclerosis.     

Use of a Vascular Skeletal Muscle Graft for Canine Esophageal Reconstruction

A technique for reconstruction of the thoracic esophagus was evaluated in 13 dogs. A 9 x 10 cm section of transversus abdominis muscle was harvested, fashioned into a tubular graft, and vascularized via the greater omentum. The vascularized tube was advanced into the caudal thorax through the diaphragm at a second surgery 20 to 44 days after harvesting the section of the transversus abdominis muscle. A 5 cm section of caudal thoracic esophagus was replaced with the vascular tubular graft. One dog died because of incarceration of the intestine within the tube and two dogs were euthanized because of infected tubes. One dog died due to an anesthetic complication, one dog had a diaphragmatic hernia and died, and one dog died from pyothorax. Leakage of luminal contents at the anastomoses did not occur in any dog. Seven dogs survived both surgeries but showed deglutitory difficulty 6 days postoperatively due to the grafted esophageal segments becoming severely strictured in each case. Angiography demonstrated vascularization of the full thickness of the graft in each of the seven dogs. An unacceptably high incidence of infection and stricture formation made this technique unsuitable for clinical use.