Diet overlap in a piscivore community

Abstract— We examined prey selection of largemouth bass ( Micropterus salmoides ), smallmouth bass ( M. dolomieu ), and yellow perch ( Percaftavescens ) by comparing diet overlap in a small, unexploited lake in Michigan, USA from 1988 to 1990. Niche hypervolume principles were applied to diet data as a means of assessing diet space for each species and the community as a whole. Largemouth bass occupied the largest proportion of community diet space (70.2%), followed by smallmouth bass (44.2%), and yellow perch (21.7%). The majority of community diet space (58.8%) was occupied by a single species, and 41.2% was shared by ≥ 2 species. Diet overlap was assessed by measuring the amount of diet space of one species occupied by the other species. Our analyses demonstrated that diet partitioning in a three species piscivore community is reflected in different use of the prey resource by co-occurring species. Niche overlap of largemouth bass with smallmouth bass and yellow perch is strongly asymmetric, largely due to the ability of largemouth bass to effectively consume prey of terrestrial origin.     

Diet of non‐native northern snakehead (Channa argus) compared to three co‐occurring predators in the lower Potomac River,USA

Abstract – Introductions of large, non‐native, carnivorous fishes continue to occur worldwide and represent a substantial management concern to global biodiversity. One of the most recent non‐native fishes to successfully establish in North America is the northern snakehead (Channa argus), found in the lower Potomac River catchment. Dispersal of the northern snakehead throughout this system has been well documented since its original discovery in May 2004; however, little is known about the foraging habits of this species and its interactions with co‐occurring predators. Here, we quantify northern snakehead diet in comparison with the diets of naturalised largemouth bass (Micropterus salmoides), and native American eel (Anguilla rostrata) and yellow perch (Perca flavescens) collected from tidal freshwaters bordering Virginia and Maryland near Fort Belvoir, Virginia. Over 97% of northern snakehead gut contents were fishes, with fundulid and centrarchid species consumed most frequently. Dietary overlap was biologically significant only between northern snakehead and largemouth bass. Aquatic invertebrates were >10 times more common in native predator diets, reducing dietary overlap with northern snakehead. Ontogenic shifts in adult northern snakehead diet were also detected, which may be explained by optimal foraging rather than true prey specificity. Northern snakehead may be occupying a novel niche based on a piscivorous diet, therefore limiting competition with resident predators in the lower Potomac River. Further research into interactions between largemouth bass and northern snakehead is needed to inform management decisions and understand the ecological impacts of this non‐native species.     

Diet of introduced largemouth bass in Korean rivers and potential interactions with native fishes

Abstract –  A dietary analysis of largemouth bass ( Micropterus salmoides ), an exotic, piscivorous species, was conducted in large South Korean river systems (>third order streams, 31 sites). Micropterus salmoides larger than 100 mm exhibited intense piscivory on native Korean juvenile fishes, with levels of piscivory amongst the highest recorded globally, for native and introduced populations. Largemouth bass exhibited an ontogenetic shift in diet, showing a progressive increase in piscivory with size, typical of this species within its native range but unlike several studies on introduced largemouth bass in Europe and Africa. Sampling of fish communities at the same sites used for diet studies showed that native piscivores and the main food fish species of largemouth bass were significantly less abundant ( P  = 0.049 and 0.045, respectively) at sites where bass were present than at sites where no bass were recorded. Largemouth bass may pose a threat to the structure of fish assemblages in Korean river systems and further study of their population interactions and how to minimise their spread is needed.     

Persistence of Florida largemouth bass alleles in a northern Arkansas population of largemouth bass, Micropterus salmoides Lacèpéde

Abstract– Lake Ashbaugh, located in northeast Arkansas, was constructed in 1981, and initially stocked with Florida largemouth bass followed by supplemental stockings of northern largemouth bass. Allele frequencies of three discriminant allozyme loci (sAAT-B, sIDH-B, sMDH-B) between Florida and northern largemouth bass were determined for 414 largemouth bass collected between 1994 and 1996. F_x bass dominated our sample, with 62.3% possessing Florida largemouth bass alleles. A high incidence of Hardy-Weinberg disequilibrium was observed, indicative of genetic change within the population. No significant differences were identified for frequency of age classes, relative weight, and length at age between the northern, F₁ and F_x phenotypes. Despite being located north of what is generally considered suitable for stocking Florida largemouth bass, it was demonstrated that temperature is not selective at present against bass possessing Florida largemouth bass alleles. However, caution should prevail when introducing non-native stock into native gene complexes, as introduced genes persist through many generations.     

Trophic ecology of speckled peacock bass Cichla temensis Humboldt 1821 in the middle Negro River,Amazon, Brazil

The aim of this study was to analyse the trophic ecology of speckled peacock bass Cichla temensis inhabiting two tributaries of the middle Negro River, the Aracá River and the Demeni River. Using an analysis of stomach contents and stable isotope composition (δ¹⁵N, δ¹³C) of scales, we describe the diet and evaluate the trophic position of subadult and adult individuals. We then test whether diet shifts and trophic positions occurred among successive size classes and among sample locations. The stomach content analysis confirmed the piscivorous feeding habit of the species and showed that the speckled peacock bass preyed on a variety species belonging to different trophic guilds. The length of the ingested prey increased with the size of the speckled peacock bass. Diet composition and trophic position were not different among size classes. δ¹³C values yielded significant shifts among the size classes: larger individuals displayed higher δ¹³C values than smaller individuals. Trophic position varied between locations, with lowest values observed in fish from the Aracá River. This study demonstrated that diet of C. temensis may vary according to the size of the fish, even at the subadult or adult stages, and according to the river/locality, even within a same basin. We then suggest that further studies take into account local availability of food resources to better explore C. temensis diet and tropic ecology.     

An evaluation of total body electrical conductivity to estimate body composition of largemouth bass,Micropterus salmoides

Measurement of total body electrical conductivity (TOBEC) recently has been used to estimate the body composition of several fish species in a noninvasive manner. The present study was conducted to evaluate the use of TOBEC in estimating body composition of largemouth bass (Micropterus salmoides). A total of 85 largemouth bass weighing 154 to 3 245 g were measured for electrical conductivity after which their proximate composition was determined by chemical means. Significant linear relationships existed between the natural logarithm of whole-body ash, lean body mass, lipid, protein, and water content and the natural logarithm of length and/or weight with r² values ranging from 0.860 to 0.999. Inclusion of the TOBEC value did not significantly improve the prediction accuracy of these models. Equations were developed to allow the prediction of body composition of largemouth bass based on length and weight measurements. Prediction models including only length and weight as variables provided estimates of body components of an independent set of fish that were not significantly different from chemically derived measurements of these components. These models should allow the rapid, nondestructive estimation of body composition of largemouth bass varying in size and condition without the added cost and processing time associated with measurement of TOBEC, although large prediction errors might prevent the detection of ecologically significant differences in body composition. However, with additional data involving narrower fish-size ranges and constant temperatures for the development of prediction equations, TOBEC may improve the prediction accuracy of body composition estimates for largemouth bass.     

Response of Largemouth Bass Micropterus salmoides to Dietary Supplementation of Lysine, Methionine, and Highly Unsaturated Fatty Acids

Abstract.— A 12-wk feeding trial was conducted in aquaria with juvenile (36.0 ± 1.2 g) largemouth bass Microptents salmoides to examine the effects of dietary supplementation of methionine, lysine, and long-chain polyunsaturated fatty acids (PUFA) on growth, feed conversion and body composition. Diets were formulated to increase dietary concentrations of methionine. lysine, and PUFAs to match levels found in whole body samples of largemouth bass. The control diet was formulated similar to diets previously tested for largemouth bass. Diets 2 and 3 were similar to the control diet hut were supplemented with 2% lysine and 1% methionine, respectively. Diet 4 was formulated to increase PUFAs, especially docosahexaenoic acid (DHA) (22:6n-3), by replacing menhaden fish oil with squid oil. Fish were fed all they would consume in 10 min, twice daily. At harvest, there were no statistically significant differences ( P > 0.05) in average individual weight or specific growth rate (SGR) among fish fed the four diets. The feed conversion ratio (FCR) of largemouth bass fed the diet supplemented with methionine (1.7) was significantly lower ( P ≤ 0.05) than fish fed the control diet (2.5). Fish fed the diet high in PUFA had significantly lower ( P ≤ 0.05) whole body lipid levels and significantly higher ( P ≤ 0.05) protein levels than fish fed the other three diets. These data indicate that the control diet in this study likely contained sufficient lysine, methionine and PUFA to meet the requirements of largemouth bass; however, additional methionine may improve feed conversion efficiency, and increased levels of PUFAs or other factors in squid oil may have a significant impact on body composition.     

Comparison of the amino acid contents of roe, whole body and muscle tissue and their A/E ratios for largemouth bass Micropterus salmoides (Lacepéde, 1802)

Nutritional amino acid requirements of varying size classes of largemouth bass were estimated using A/E ratios. Nutrient and amino acid contents of roe and carcass of the different size classes were determined and compared to results of selected, classic works related to the concept of ideal protein and relationship between the contents of individual, essential amino acids and the total contents of essential amino acids – A/E ratios. Protein content in the roes of the largemouth bass were higher in comparison to the carcass, but the content of lipids of the roes and of the carcass didn’t present significant difference (P<0.05). Largemouth bass showed higher muscle protein content in comparison to other species. Although some authors report variation in the contents of some amino acids in the carcass of selected species, differences observed in this study regarding carcass amino acid contents of tilapia, speckled catfish and largemouth bass were not significant (P<0.05), values of the ratio A/E followed the same trend. Results, herein presented, indicate that the amino acid profile of largemouth bass could be used as complementary tool for balancing amino acids in formulated feed for the species, and in the validation of amino acid requirements determined in performance studies.     

Patterns of movement of centrarchids in two warmwater streams in eastern Tennessee

We studied the movements of redbreast sunfish (Lepomis auritus), bluegill (Lepomis macrochirus), rock bass (Ambloplites rupestris), largemouth brass (Micropterus salmoides), and warmouth (Lepomis gulosus) by tagging fishes with individually numbered tags. We sampled quarterly over a 3-year period (1985–1988) at six 800- to 2300-m-long sites in 2 streams near Oak Ridge, Tennessee. Overall, distances between successive captures were < 100 m for approximately two-thirds of all fishes. Only rarely did we document movements over long distances; distances of 17.0-17.6 km between successive captures were obtained for 5 fishes (1 redbreast sunfish, 1 largemouth bass and 3 bluegill), and only one other fish moved > 10 km. In general, all species were highly sedentary in both streams, with rock bass and redbreast sunfish being the most sedentary and bluegill and largemouth bass being the least. In most instances, redbreast sunfish showed the same distribution of distances between captures regardless of the interval between captures (1 week, 3 months, 6 months or more). This species also tended to be more mobile in the spring and less mobile in the winter, with large fish exibiting the greatest distances between captures. Some redbreast sunfish showed a very restricted home range, being captured 3 or more times over ≥ 6 months in the same 50-m section of stream. A few individuals moved 4-9 km only to return to their original location, but most fish exhibited behaviors intermediate between these extremes. For all species, upstream and downstream movements were equally common, although movements in one direction predominated at some sites for limited periods of time. Knowledge of movement patterns and differences in behavior among species is important not only in understanding the basic ecology of the species but also in designing, conducting and interpreting environmental monitoring studies.     

Leech (Myzobdella lugubris) infestations in largemouth bass (Micropterus salmoides) in Back Bay,Virginia, USA

Back Bay is an oligohaline, coastal bay in southeast Virginia, USA. Since 2004, leeches have been observed in the oral cavities of largemouth bass (Micropterus salmoides) in this body of water. Leeches (Myzobdella lugubris) have previously been documented in the oral cavities of largemouth bass in the Currituck Sound, which is confluent with Back Bay on its southern border. Supplemental stocking of largemouth bass in Back Bay since 2009 has resulted in an increasing population; however, concern exists that leech infestation may be negatively affecting health of larger fish, which are still less abundant than expected. Despite the wide distribution of this leech, there is little available literature regarding its health impacts on hosts. In this study, we examine potential impacts of oral leech infestations on stress markers and haematological parameters of largemouth bass in Back Bay. No significant changes in plasma glucose or cortisol were observed between leech‐infested and uninfested fish, and haematological parameters were not significantly different between the groups. Further, there was no evidence of systemic infections associated with leech infestation.     

Effects of Different Feed Habituation Methods on Survival and Feed Habituation Success of Largemouth Bass,Micropterus salmoides,in a Recirculating Aquaculture System

Demand for food size and trophy largemouth bass, Micropterus salmoides, is steadily increasing. However, largemouth bass, a carnivorous species, are more difficult to feed habituate than other commercially produced fish. Improving current feed habituation techniques to increase the percentage of fingerlings successfully feed habituated would increase profits, productivity and efficiency, and available product in the largemouth bass industry. The focus of this research was to evaluate different combinations of automatic feeders and supplemental hand feeding to determine the feed habituation success under commercial largemouth bass production conditions. Hand feeding, using only automatic feeders, or combinations of both, did not result in significant differences with regard to survival or feed habituation success. Initial fingerling size (total length, TL) was found to be the most important factor affecting survival and feed habituation success. Fish ranging from 40 to 50 mm (TL) survived and feed habituated at significantly higher rates than 30–40 and 50–60 mm (TL) fingerlings. Medium‐sized fish provide the best results in terms of feed habituation success and survival. The results suggest any combination of hand feeding and automatic feeders may be used to achieve feed habituating success in largemouth bass.     

Activity patterns of largemouth bass in a subtropical US reservoir

Abstract  Nine largemouth bass, Micropterus salmoides Lacepède, >1.5 kg were implanted with radio tags and tracked for 1 year in an embayment of Lake Seminole, Georgia, USA. Most largemouth bass movement was <50 m h⁻¹ in all seasons and at all times of day. During the day largemouth bass were offshore in deeper water near large woody structures and moved little. Movement was lower during dusk and night periods, and a general movement towards shoreline areas was evident. Water depths where largemouth bass were located had bimodal distributions in all seasons and diel periods, with one mode typically at depths <1 m and another around 3 m. Home ranges were typically largest in the summer and smallest in the winter. Largemouth bass appeared to divide their time between an offshore resting area, primarily occupied during the day, and a near-shore area, where foraging presumably occurred, primarily used during low-light periods. The largemouth bass population in Lake Seminole consists of multiple overlapping subpopulations, offering the opportunity for embayment-specific management.     

Trophic plasticity of invasive juvenile largemouth bass Micropterus salmoides in Iberian streams

Biological invasions are a major factor for biodiversity loss, particularly in freshwater environments. Largemouth bass Micropterus salmoides is native to North America and is invasive on the Iberian Peninsula, primarily to provide angling opportunities in reservoirs. However, this species is a threat to the endemic Iberian fauna via predation and competition. Currently, there is little information on largemouth bass in European streams. Thus, we assessed the trophic plasticity and body condition of young largemouth bass in both invasive (the regulated Bullaque River) and native (Murray Creek) streams. Abundance of juvenile largemouth bass, percentage of full stomachs and body condition were higher in Bullaque River. Largemouth bass preyed on benthic invertebrates much more heavily in the Bullaque River, whereas fishes were the most important prey in Murray Creek. Prey richness, diet diversity and trophic niche breadth were higher in the Bullaque River population. Largemouth bass preferred water-column fishes as prey and avoided consuming benthic fishes in Murray Creek, whereas water-column fishes were avoided in Bullaque River. These results demonstrate that largemouth bass display substantial trophic plasticity which possibly facilitates its success as invasive species. Regulated Iberian streams may provide both suitable food and habitat resources with minimal predation pressure, and hence may serve as recruitment sources for this invasive fish.     

摄食不同饵料的大口黑鲈肠道菌群分析

为了探究投喂冰鲜杂鱼和人工配合饲料对大口黑鲈肠道菌群的影响,本研究采用变性梯度凝胶电泳技术(PCR-DGGE)对摄食不同饵料的2组鲈鱼肠道内容物的细菌群落结构进行了比较分析.结果显示,冰鲜组与饲料组的大口黑鲈肠道菌群组成相似性较低(相似性24％),且冰鲜组肠道菌群多样性高于饲料组.对2组大口黑鲈中共有条带和特异性条带进一步测序分析显示,2组大口黑鲈肠道共有菌有红螺菌、梭菌、保科爱德华菌、嗜麦芽窄食单胞菌和蓝细菌.冰鲜组大口黑鲈肠道中的特异性菌归属为拟杆菌、肉食杆菌、魏斯氏菌和邻单胞菌;饲料组大口黑鲈肠道中的特异性菌归属为黄杆菌、鲸杆菌、柠檬明串珠菌、缺陷短波单胞菌.研究表明,与投喂冰鲜杂鱼的大口黑鲈相比,人工配合饲料降低了大口黑鲈肠道菌群多样性,也抑制了拟杆菌等有益菌在大口黑鲈肠道内的分布.推测饲料组大口黑鲈生长较慢可能与其肠道菌群中拟杆菌与厚壁菌的比值下降有关.研究结果提示,在今后大口黑鲈养殖或者人工配合饲料研制过程中,添加拟杆菌等益生菌也许可促进大口黑鲈的生长和营养吸收.     

Comparison of the pathogenicity of Francisella orientalis in Nile tilapia (Oreochromis niloticus), Asian seabass (Lates calcarifer) and largemouth bass (Micropterus salmoides) through experimental intraperitoneal infection

Francisella orientalis is a highly virulent, emerging bacterium that causes mass mortalities in tilapia. This pathogen also affects numerous other warm-water fish species, including three-line grunt, hybrid striped bass and various ornamental fish. This study sheds light on two new species of fish that are susceptible to F. orientalis. Asian seabass and largemouth bass showed variable levels of susceptibility in a bacterial challenge experiment. After intraperitoneally injected with a dose of 10⁶ CFU/fish, a total of 64.28% and 21.42% mortalities were obtained in Asian seabass and largemouth bass, respectively. Meanwhile, Nile tilapia showed acute mortality of 100%. All fish showed typical lesions of francisellosis, including multifocal granulomas in the spleen and head kidney. Immunohistochemical analysis revealed strong positive signals inside the granulomas of all fish. The bacterial recovery in solid media from infected fish was highest in Nile tilapia (85.71%), followed by Asian seabass (35.71%) and largemouth bass (21.42%). PCR results tested 100% positive for Nile tilapia, and 78.57% and 21.42% for Asian seabass and largemouth bass, respectively. In conclusion, Asian seabass and largemouth bass are susceptible to this pathogen, which warrants new management strategies when employing predation polyculture systems of these species with tilapia.     

Diet dynamics of the adult piscivorous fish community in Spirit Lake, Iowa, USA 1995–1997

Abstract – Diets of adults of six important piscivorous fish species, black crappie Pomoxis nigromaculatus , largemouth bass Micropterus salmoides , northern pike Esox lucius , smallmouth bass Micropterus dolomieui , walleye Stizostedion vitreum , and yellow perch Perca flavescens were quantified in Spirit Lake, Iowa, USA from May to October in 1995–1997. Forty-one prey taxa were found in the diets of these species, including 19 species of fish. The most important prey taxa overall were yellow perch, amphipods and dipterans. Diets of northern pike and walleye were dominated by yellow perch. Largemouth bass diets included large percentages of both yellow perch and black bullhead Ameiurus melas . Smallmouth bass diets included large percentages of both yellow perch and crayfish. Black crappie and yellow perch diets were dominated by invertebrates, primarily amphipods and dipterans. There were pronounced differences in diets among species, among size classes within species and over time. Most of the dominant prey taxa we documented in the diets of piscivorous species were in accordance with previous studies, but a few deviated significantly from expectations. Many of the temporal diet changes were asynchronous among piscivorous species and size classes, suggesting different responses to common prey resources over time.     

Effect of body colour of Oreochromis mossambicus (Peters) on predation by largemouth bass, Micropterus salmoides (Lacepède)

Abstract. Fifty each of F₂-generation gold ( gg ), bronze ( Gg ), and black ( GG ) Oreochromis mossambicus (Peters) and either zero, four or eight largemouth bass, Micropterus salmoides , (Lacepède) were stocked in 20-m² concrete tanks. After 7 days, water in all tanks was drained, and fish in each tank were censused. Largemouth bass ate 18% of the tilapia in the four-bass treatment (1 tilapia/bass/day) and 60% of the tilapia in the eight-bass treatment (1·6 tilapia/bass/day); the difference was significant (P=0–05). In the four-bass treatment there was a greater observed mean predation rate on gold than that on black or on bronze tilapia, but the difference was not significant: largemouth bass ate 25% of the gold tilapia, 16% of the bronze tilapia, and 13% of the black tilapia. In the eight-bass treatment, predation on the gold tilapia was significantly greater than that on both bronze (P=0·05) and on black (P=0·06) tilapia; predation on bronze and black tilapia was similar: largemouth bass ate 80% of the gold tilapia, 48% of the bronze tilapia, and 51% of the black tilapia. Overall average total predation (both treatments combined) on gold tilapia was significantly (P=0·06) greater than that on both bronze and on black tilapia, which did not differ: largemouth bass ate 52% of the gold tilapia, 32% of the bronze tilapia, and 32% of the black tilapia. The increased vulnerability of gold tilapia to predation was a negative pleiotropic effect of the gg genotype.     

饲料中添加复合植物提取物对大口黑鲈生长性能、血清生化指标和肝脏、肠道健康的影响

为研究饲料中添加由桑叶黄酮、栗木单宁和桑叶多糖组成的复合植物提取物对大口黑鲈生长性能、血清生化指标和肝脏、肠道健康的影响,实验设计3个处理组,每组4个重复,分别投喂添加0、0.1%、0.2%复合植物提取物的等氮等脂饲料(J0、J1、J2),养殖周期为56 d。结果显示,与J0组相比,J2组大口黑鲈的末均重、增重率及特定生长率分别显著提高8.34%、11.24%和5.88%;3组大口黑鲈机体粗脂肪、水分、粗灰分、钙和总磷,肥满度、脏体比均无显著差异,粗蛋白含量随着复合植物提取物添加量的增加呈上升趋势,与J0组相比,J2组显著提高5.61%;J1和J2组肝体比显著降低9.10%～10.28%,血清白蛋白含量和白蛋白/球蛋白值显著提高8.28%～12.62%和13.56%～23.73%,J2组血清球蛋白含量显著降低9.47%,J1和J2组血清谷丙转氨酶、谷草转氨酶活性和血糖含量分别显著降低31.25%～46.88%、7.69%～12.54%和20.90%～22.40%;J1和J2组胆固醇和甘油三脂含量呈降低趋势,高密度脂蛋白胆固醇和低密度脂蛋白胆固醇呈升高趋势;J1和J2组肝脂空泡化程度显著...     

配合饲料和冰鲜鲢对大口黑鲈生长、血浆生化指标、抗氧化能力和组织学的影响

为探讨不同来源饲料对大口黑鲈生长、血浆生化指标、抗氧化能力以及肝脏和肠道组织学的影响,实验选取初始体质量为(12.45±0.07)g的大口黑鲈180尾,随机分成2个处理,每个处理3个重复,每个重复30尾,分别投喂配合饲料和冰鲜鲢,养殖84 d。结果显示,配合饲料组大口黑鲈的增重率(WGR)、特定生长率(SGR)和饲料系数(FCR)显著低于冰鲜鲢组,而蛋白质效率(PER)、肝体比(HSI)和脏体比(VSI)显著高于冰鲜鲢组。大口黑鲈摄食配合饲料后,肝糖原含量显著高于冰鲜鲢组,肝脏蛋白酶活性显著低于冰鲜鲢组,而肌糖原和肠淀粉酶活性无显著差异。饲喂配合饲料组大口黑鲈血浆谷草转氨酶(AST)活性、血糖和丙二醛(MDA)含量以及Ca/P比值显著高于冰鲜鲢组,而血浆碱性磷酸酶(ALP)、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性以及总蛋白(TP)、甘油三酯(TG)、尿素、钙和磷含量显著低于冰鲜鲢组。各实验组血浆谷丙转氨酶(ALT)活性以及胆固醇(TC)和胰岛素含量均无显著差异。配合饲料明显影响大口黑鲈肝脏和肠道的组织结构,饲喂配合饲料组实验鱼的肝脏空泡化现象严重,小肠绒毛受到严重机械性损伤。研究表明,在本实验条件下,冰鲜鲢更适合饲喂大口黑鲈,配合饲料不仅影响大口黑鲈的生长,而且损伤肝脏和肠道的健康。因此,可以借鉴冰鲜鲢的营养组成和大口黑鲈的代谢特性,深入研发大口黑鲈的饲料配制技术。     

驯食配合饲料的大口黑鲈3个选育世代的遗传多样性分析

为了检测驯食配合饲料的大口黑鲈(Micropterus salmoides)3个选育世代群体遗传多样性水平变化,利用微卫星标记技术对驯食配合饲料大口黑鲈选育基础群体(Sp0)和第二、三和四代选育群体(Sp2、Sp3和Sp4)共240尾样品进行检测。结果显示,18个微卫星位点共获得44个等位基因。Sp0、Sp2、Sp3和Sp4的平均观测杂合度(H_o)分别为0.4895、0.4802、0.4579和0.4206,平均期望杂合度(H_e)分别为0.4615、0.4454、0.4621和0.3916,平均多态信息含量(PIC)分别为0.3791、0.3659、0.3764和0.3257。4个群体间的配对比较群体间遗传分化指数(F_(st))值在0.01612～0.16162之间、遗传距离(D_a)在0.0249～0.1434之间。遗传变异来源(AMOVA)分析显示,只有8.38%的变异来自于群体间,其余遗传变异均来自于个体间。研究表明,经连续多代选育之后,易驯食配合饲料的快长大口黑鲈选育群体具有中度遗传多样性,具备选育潜力,可继续进行选育。