长期施肥对高原农业生态系统土壤有机碳和氮的影响

The effects of fertilization on the distributions of organic carbon (OC) and nitrogen (N) in soil aggregates and whether these effects vary with cropping system have not been well addressed.Such information is important for understanding the sequestration of OC and N in agricultural soils.In this study,the distributions of OC and N associated with soil aggregates were analyzed in different fertilization treatments in a continuous winter wheat cropping system and a legume-grain rotation system in a 27-year field experiment,to understand the effects of long-term fertilization on the distributions of OC and N in aggregates and to examine the recovery of soil OC and N in a highland agroecosystem.Manure fertilizer significantly decreased soil bulk density but increased the amount of coarse fractions and their associated OC and N stocks in the soils of both systems.Fertilizers N + phosphorus (P) and manure had similar effects on total soil OC and N stocks in both systems,but had larger effects on the OC and N stocks in ＞ 2 mm aggregates in the legume-grain rotation system than in the continuous winter wheat system.The application of P increased the OC and N stocks in ＞ 2 mm aggregates and decreased the loss of N from chemical fertilizers in the legume-grain rotation system.The results from this study suggested that P fertilizer should be applied for legume-included cropping systems and that manure with or without chemical fertilizers should be applied for semiarid cropping systems in order to enhance OC and N accumulation in soils.     

长期施用化肥和有机肥对太湖地区水稻土有机碳特征的影响

A long-term experiment set up in 1980 compared the effects of applying manures and chemical fertilizers on a paddy soil in the Taihu Lake region,China.Of the fourteen randomly distributed treatments consisting of different combinations of organic manure,inorganic nitrogen (N),phosphorus (P),and potassium (K),and rice straw,eight were selected for the present study in 2007.Application of organic manure plus straw significantly increased soil organic carbon (SOC) content of the topsoil (0-10 cm) compared to that of chemical fertilizers alone.The content of SOC was relatively stable in the 10-30 cm layer in the chemical fertilizer treatments and in the 20-40 cm layer in the manure treatments.The stable carbon isotope ratio (δ 13 C) ranged from 24‰ to 28‰ and increased gradually with depth.The content of SOC was significantly (P < 0.05) negatively correlated with δ 13 C.In the 0-20 cm layer,the δ 13 C value significantly decreased in the treatments of manure alone (M),manure and chemical N and P fertilizers (MNP),manure and chemical N,P,and K fertilizers (MNPK),manure,rice straw,and chemical N fertilizer (MRN),and chemical N fertilizer and rice straw (CNR),as compared with the no-fertilizer control.In the 30-50 cm layer,however,the ratio significantly increased in all the treatments except Treatment CNR.Mineralization of organic C peaked in the first 2-4 d of incubation and gradually leveled off thereafter over the first 3 weeks,being faster in the manure treatments than the chemical fertilizer treatments.The average rate of mineralization varied from 55.36 to 75.46 mL CO 2 kg-1 d-1 and that of stable mineralization from 10 to 20 mL CO 2 kg-1 d-1.In eight weeks of incubation,cumulative mineralization was always higher in the manure treatments than the chemical fertilizer treatments,being the highest in Treatment MRN.Combined humus in the soil was mainly (over 50%) composed of tightly combined fraction.The loosely combined humus and its ratio of humic acid (HA) to fulvic acid (FA) significantly increased with long-term application of organic manure and chemical fertilizers.It could be concluded that the cycle of organic C in the paddy soil ecosystem studied was stable over the long-term application of fertilizers and continued cultivation.     

长期施肥对红壤性水稻土有机碳动态变化的影响

在23年的田间定位试验区,研究了长期施肥对红壤水稻土不同发生层有机C含量及其储量动态变化的影响.结果表明,在不施肥(CK)、无机肥(NPK)、有机肥(猪粪 紫云英绿肥,OM)和无机肥与有机肥配施(NPKM)处理中,土壤有机C的积累过程还处于稳定增长的阶段,特别是施有机肥处理(OM和NPKM)其增加趋势与利用年限呈显著的直线相关.各施肥处理土壤剖面不同层次有机C含量都呈如下的顺序:A＞P＞W1、W2,表现出随着剖面的加深有机C含量逐渐降低的趋势,长期施肥对土壤有机C含量的影响主要表现在表层.随着年限的增加,土壤有机C储量都有增加的趋势.不同施肥处理土壤剖面4层(A、P,W1和W2)土壤有机C储量都表现出NPKM＞OM＞NPK＞CK的趋势,施入有机肥促进了土壤有机C储量的增加.     

不同施肥处理对红壤性水稻土团聚体的分布及有机碳、氮含量的影响

土壤采自江西省红壤研究所历经22年的肥料定位试验地,各施肥处理为:不施肥(CK)、施N肥(N)、施P肥(P)、施K肥(K)、施N、P肥(NP)、施N、K肥(NK)、施N、P、K肥(NPK)、施双倍N、P、K肥(2NPK)、施N、P、K肥和有机肥(NPK OM).研究了不同施肥处理对水稻土团聚体分布的影响以及土壤有机C、N在各级团聚体上赋存的情况.研究结果表明:有机C、N主要分配在2～0.25 mm的大团聚体上,C/N比随着团聚体粒级的减少而降低;水稻土团聚体的形成与有机C(SOC)密切相关,表现出与“层次性“机制相符的现象;施P、K、NK肥和NPK OM显著提高大团聚体的含量和团聚体的稳定性;NPK OM也显著提高土壤有机C、N和各级团聚体上的有机C、N的含量.     

不同施肥处理对红壤水稻土团聚体有机碳分布的影响

通过23年的长期田间定位试验观测,研究了施肥对红壤水稻土不同发生层团聚体组成、有机碳含量及有机碳库分布的影响.试验设不施肥（CK）、无机肥（NPK）、有机肥（猪粪＋紫云英）（OM）和无机肥与有机肥配施（NPKM）等4个处理.结果表明,＞3 mm团聚体含量随土层深度呈增加趋势,而其他粒径的团聚体含量则呈下降趋势.施肥处理有利于1～3 mm和0.25～1 mm团聚体含量增加.各处理中,各层同粒径团聚体有机碳含量从高到低的顺序为：A＞P＞W1、W2.不同粒径团聚体中有机碳含量有明显差异,除＜0.05 mm团聚体外,粒径愈细,有机碳含量愈高.1～3 mm和0.25～1 mm团聚体含量与全土有机碳含量呈显著正相关.不同施肥处理有机碳储量表现出NPKM＞OM＞NPK＞CK的趋势.同施肥处理同发生层不同粒径团聚体有机碳储量从高到低的顺序为：＞3 mm、1～3 mm、0.25～1 mm、0.05～0.25 mm和＜0.05 mm,且差异显著.施肥处理增加的新碳主要向1～3 mm、0.25～1 mm团聚体富集.     

长期施肥对红壤性水稻土团聚体活性有机碳的影响

在23年的长期田间定位试验区,研究了长期施肥对红壤性水稻土团聚体活性有机碳含量的影响。结果表明,在不施肥(CK)、无机肥(NPK)、有机肥(猪粪+紫云英绿肥)(OM)和无机肥与有机肥配施(NPKM)处理中,土壤团聚体活性有机碳含量均随深度的增加而降低。长期施用肥料,特别是有机肥与无机肥配施会提高土壤团聚体活性有机碳含量,从而保持和提高土壤有机碳库质量。不同粒级土壤团聚体中活性有机碳含量和团聚体活性有机碳占团聚体有机碳比率有差异,潜在可矿化碳含量和潜在可矿化碳占团聚体有机碳比率从高到低的顺序为:0.25~1mm、1~3mm、>3mm、0.05~0.25mm和<0.05mm;而可溶性有机碳含量和可溶性有机碳占团聚体有机碳比率从高到低的顺序为:0.05~0.25mm、0.25~1mm、1~3mm、>3mm和<0.05mm。不同施肥处理A层土壤团聚体潜在可矿化碳、可溶性有机碳含量都与土壤团聚体有机碳含量都呈极显著相关;P层除1~3mm团聚体外都呈显著相关。土壤微团聚体(<0.25mm)中有机碳的稳定性高于大团聚体(>0.25mm)。     

施肥对红壤性水稻土有机碳活性和难降解性组分的影响

以稻田22 a施肥定位实验为样地,分别采用硫酸和盐酸加热水解两种方法,研究施肥对水稻土有机碳活性和难降解性组分的影响。施肥处理包括不施肥对照(CK)、单施氮肥(N)、单施磷肥(P)、单施钾肥(K)、氮磷肥混施(NP)、氮钾肥混施(NK)、氮磷钾肥混施(NPK)、双倍氮磷钾肥混施(2NPK)、氮磷钾和有机肥配施(NPK+OM)。结果表明,两种酸解法获得的有机碳组分结果趋势一致,所得结果高度相关(p<0.05)。与对照相比,施肥总体上有助于土壤总有机碳的积累;除了单施钾肥处理外,其他施肥处理均提高了土壤活性有机碳的含量,但单施氮及NPK+OM处理显著提高了土壤有机碳活性指数(LIC),并以NPK+OM的促进作用最显著(p<0.05)。与对照相比,除了单施氮处理外,其他施肥处理下土壤难降解有机碳(酸解残余碳)含量均呈增加趋势,也以NPK+OM的作用最明显(p<0.05);单施钾肥显著提高有机碳的难降解性(p<0.05),但单施氮和NPK+OM处理显著降低有机碳的难降解性指数(R IC,p<0.05)。施氮肥和有机肥对土壤总有机碳含量的促进作用主要体现在对活性有机碳的贡献上。因此,可以通过优化施肥措施(如选择合适有机肥种类、减施氮肥和增施钾肥等)来调控水稻土有机碳的含量及稳定性。     

长期施肥对红壤不同形态碳的影响

长期定位施肥对红壤不同形态有效碳的影响试验研究结果表明,与对照相比,长期施肥后红壤不同形态有效碳均有不同程度提高,且全C、矿化碳的变化为厩肥>绿肥>稻草秸秆肥>本田还田>对照,易氧化碳、C库管理指数(CPMI)、C素有效率的变化为绿肥>稻草秸秆肥>厩肥>本田还田>对照,微生物生物量C变化为绿肥>厩肥>稻草秸秆肥>本田还田>对照,绿肥和稻草秸秆肥可有效改善土壤肥力及理化性状。C库管理指数与土壤养分因子量相关或极相关关系,反映了农业生产措施对土壤C库的影响,可运用其评估土壤C库的变化。     

后续施肥措施改变对红壤性水稻土团聚体有机碳组分的影响

利用一个长达30年且已进行适当变更的长期定位施肥试验,采用物理方法对团聚体有机碳进行分组,并运用δ13 C自然丰度方法,研究长期施用高量有机肥、常量有机肥、化肥及当其施肥措施改变(化改常、常改高、高改化、常改化)3年后红壤性水稻土团聚体有机碳组分含量及其分布比例的变化规律,以期为调控稻田土壤肥力及红壤性水稻土有机碳库的管理提供理论依据。结果表明:与施用化肥30年相比,长期施用有机肥显著提高了红壤性水稻土团聚体总有机碳、粗游离态颗粒有机碳(cfPOC)、细游离态颗粒有机碳(ffPOC)、闭蓄态颗粒有机碳(oPOC)及矿物结合态有机碳(MmMOC)的含量,其中以游离态颗粒有机碳变幅最大,达67.5%~150.0%,对施肥最敏感,能较好地反映长期施肥下土壤有机碳库的变化。在后续施肥过程中,增加有机肥施入量(化改常、常改高)团聚体总有机碳、粗游离态颗粒有机碳(cfPOC)、细游离态颗粒有机碳(ffPOC)、闭蓄态颗粒有机碳(oPOC)及矿物结合态有机碳(MmMOC)含量将分别显著提高5.2%~15.5%,2.8%~40.2%,18.9%~43.9%,2.8%~17.6%,5.1%~8.2%;而减少有机肥施入量(高改化、常改化)则与之相反,分别降低15.8%~20.9%,12.6%~26.9%,24.6%~48.4%,19.9%~23.9%,4.9%~21.9%。在所有施肥处理条件下闭蓄态颗粒有机碳(oPOC)分布比例最低,为11.3%~13.4%;矿物结合态有机碳(MmMOC)分布比例最高,为50.4%~59.0%,是红壤性水稻土固存有机碳的主要形式。外源新碳施入量越多,大团聚体及其各有机碳组分的新碳含量越高,且45.6%~50.1%进入矿物结合态有机碳组分,34.1%~42.3%进入游离态颗粒有机碳组分,11.8%~18.0%进入闭蓄态颗粒有机碳组分。因此,在我国南方红壤性稻作区的农业生产过程中应继续或加大施用有机肥,从而进一步维持或提升土壤不同组分有机碳库。     

地下水位和长期施肥对红壤性水稻土有机碳矿化特征的影响

以1982年开始的长期定位试验红壤性水稻土为对象,采用室内模拟培养试验方法,对耕层土壤有机碳矿化特征进行分析,以探究两个地下水位(20 cm、80 cm)下,长期不同施肥处理(高量有机肥+化肥(2/3OM)、常量有机肥+化肥(1/3OM)、单施化肥(NPK))红壤性水稻土有机碳矿化特征和差异。结果表明,较高的地下水位促进土壤有机碳累积,提高了高量有机肥和常量有机肥处理的土壤有机碳矿化速率,且增加了其有机碳累积矿化量(C_t)及潜在可矿化有机碳量(C_0),但土壤有机碳累积矿化率在施用化肥的条件下表现为减少。长期不同施肥的红壤性水稻土土壤有机碳矿化特征在不同地下水位条件下变化明显,2个地下水位的土壤有机碳含量、土壤有机碳矿化速率及有机碳累积矿化量高低排序均表现为:2/3OM1/3OMNPK;20cm地下水位长期施用2/3OM和1/3OM土壤有机碳累积矿化率分别较NPK高出53.32%(P0.05)、15.44%,80 cm地下水位则分别低出5.56%、17.95%(P0.05);20 cm地下水位2/3OM和1/3OM处理的土壤潜在可矿化有机碳量(C_0)显著高于NPK处理,而80cm地下水位C_0表现为1/3OM处理显著低于NPK处理;地下水位对相同施肥处理土壤有机碳周转常数(k)影响不明显,而在同为80 cm的地下水位条件下,长期施用有机肥可显著提高k。因此,长期不同施肥对红壤性水稻土有机碳累积矿化率、潜在可矿化有机碳量及周转常数的影响在不同地下水位条件下发生变化。     

长期施肥对华北平原一种潮土有机氮组成的影响

In order to illustrate the change of nitrogen （N） supply capacity after long-term application of manure and chemical fertilizer, as well as to properly manage soil fertility through fertilizer application under the soil-climatic conditions of the North China Plain, organic N forms were quantified in the topsoil with different manure and chemical fertilizer treatments in a 15-year fertilizer experiment in a Chinese calcareous alluvial soil. Soil total N （TN） and various organic N forms were significantly influenced by long-term application of chemical fertilizer and manure. TN, total hydrolysable N, acid-lnsoluble N, amino acid N and ammonium N in the soil increased significantly （P 〈 0.05） with increasing manure and fertilizer N rates, but were not influenced by increasing P rates. Also, application of manure or N fertilizer or P fertilizer did not significantly influence either the quantity of amino sugar N or its proportion of TN. Application of manure significantly increased （P 〈 0.05） hydrolysable unknown N, but adding N or P did not. In addition, application of manure or N fertilizer or P fertilizer did not significantly influence the proportions of different soil organic N forms.     

添加牛粪对长期不同施肥潮土有机碳矿化的影响及激发效应

为了探讨长期不同施肥潮土有机碳矿化对添加牛粪的响应特征及添加牛粪对长期不同施肥潮土有机碳矿化的激发效应,以始建于1986年的长期定位试验为平台,通过室内恒温培养的方法研究添加等氮量牛粪后长期不同施肥(不施肥,CK;常量有机肥,SMA;常量化肥,SMF;常量有机无机配施,1/2(SMA+SMF))潮土有机碳矿化、土壤有机碳及活性碳库组分(微生物量碳、可溶性有机碳、颗粒有机碳和易氧化有机碳)含量的变化特征。结果表明:无论添加牛粪与否,长期不同施肥潮土有机碳矿化过程均符合一级动力学方程,而牛粪的添加显著增加了长期不施肥、长期单施化肥和长期有机无机配施土壤的有机碳矿化速率常数,增长幅度分别为21.74%、35.00%和45.00%;添加牛粪提高了长期不同施肥潮土有机碳、微生物量碳、颗粒有机碳和易氧化有机碳含量,却显著降低了可溶性有机碳含量;牛粪对长期不施肥、长期施用常量有机肥、常量化肥和常量有机无机配施潮土有机碳矿化的正激发效应分别达到了48.56%、3.60%、48.43%和3.92%,且对长期不施肥及长期施用常量化肥潮土的激发效应显著高于对长期施用常量有机肥及长期有机无机配施土壤;冗余分析显示添加牛粪对长期不同施肥土壤有机碳矿化的激发效应与土壤活性组分碳氮比呈正相关,与土壤养分含量呈负相关。该研究不仅为合理施用有机肥和实现农田生态系统的可持续发展提供理论依据,还有利于实现农业资源再利用及其效益最大化。     

长期有机无机肥配施下红壤性稻田水稻产量及土壤有机碳变化特征

利用中国农业科学院红壤实验站红壤稻田长期定位试验,研究了长期有机无机肥配施下双季稻增产潜力和土壤有机碳变化特征。31年的试验结果表明,1）施肥能促进水稻早晚稻稻谷和地上部产量增加,其中,有机肥配施均衡的NPK处理促进作用最大,NPKM处理下稻谷年均产量比NPM、 NKM、 PKM、 M和NPK分别高 5.8%、 10.9%、 16.2%、 15.9%和20.4%。2）施肥能促进水稻土有机碳含量增加,其中,有机肥配施均衡的NPK处理提升效果最为明显, NPKM处理下所测年度土壤有机碳平均含量比NPM、 NKM、 PKM、 M和NPK分别高出2.5%、 3.5%、 2.0%、 0.6%和 32.8%。3）随着试验的进行,单施有机肥对早、 晚稻稻谷和地上部产量的促进效果逐步优于单施化肥氮、 磷、 钾处理（NPK）,对土壤有机碳的提升效果也明显优于单施化肥氮、 磷、 钾。红壤性稻田双季稻生产实践中,有机无机肥配施模式值得推荐,但需均衡配施化肥氮、 磷、 钾。     

长期施肥对棕壤有机碳组分的影响

对起始于1979年的棕壤长期肥料定位试验田2005年的耕层土壤不同有机碳组分进行了测定与分析,以探讨长期施肥影响土壤有机碳的过程及机理。结果显示:长期单施化肥降低了土壤的游离态颗粒有机碳（FPOM-C）含量,但进一步稳定了矿物结合态有机碳(MOM-C),最终提高了土壤总有机碳(TOC)含量;长期施用有机肥和有机肥配施化肥使土壤的FPOM-C、闭蓄态颗粒有机碳(OPOC)、MOM-C以及含量均显著提高,且增加效果好于单施化肥。从各组分有机碳所占比例或相对比值来看,长期施用有机肥和有机肥配施化肥提高了POM-C/TOC比例而降低了MOM-C/TOC比例,使FPOM-C/OPOM-C比值显著增大。表明土壤有机碳结构分组的应用有助于揭示长期施肥影响土壤有机碳的机理。     

Effects of long-term fertilization with different substitution ratios of organic fertilizer on paddy soil

In recent years, the abuse of chemical fertilizers has caused numerous environmental problems, such as soil acidification and compaction. Replacing chemical fertilizers with organic fertilizers can effectively alleviate these problems. However, the effects of alternative organic fertilizers remain unclear. To explore the effects of organic fertilizer substitution on rice yield and paddy soil physicochemical properties and bacterial community structure, we conducted a 5-year experiment using different proportions of organic fertilizer substitution in a double-cropping rice field in Jiangxi, China. Our results showed that replacing chemical fertilizers with organic fertilizers can reduce soil acidification, increase soil organic matter content, nutrient contents, and enzyme activities, improve soil physicochemical properties and microbial community, and enhance soil metabolism. Appropriate organic substitution also had positive effects on rice production. These findings enhance our understanding of the effects of different alternative organic fertilization methods and have important theoretical significance for the promotion of the use of organic fertilizers in the future.     

Effect of some cations,anions, and organic residues on potassium leaching and fractionation in calcareous sandy loam soil

Potassium (K) is one of the essential elements for plants. There has been enough research to determine pollution of nitrogen (N), phosphorus (P), and heavy metals in soil. However, by comparison research on the storage and transport of K has been neglected. Chemical fertilizer usage leads to serious environmental problems in Iran. Leaching of K can be affected by type of anions and cations present in the chemical fertilizers. Potassium leaching experiments were performed using 10 mM NH₄Cl, (NH₄)₂HPO₄, NH₄H₂PO₄, NH₄NO₃, NH₄OAC, CaCl₂, Ca(NO₃)₂, NaNO₃, and CO(NH₃)₂. The leaching experiment was lasted for 20 days (15 pore volumes). In addition, a set of experiments were conducted, where potato and wheat residues and poultry manure and sheep manure were added to soil at the rate of 5% and distilled water was used as the leaching solution to investigate impacts of organic residues on K leaching. In general, maximum K release was observed using NH₄Cl (566 kg ha^?1). Potato and wheat residues had maximum and minimum impacts on K leaching, respectively. Potassium fractionation was carried out after the end of the leaching experiment. The results indicated that leaching of soil in the presence of soluble salts and organic residues altered K distribution in different parts of soil.