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采用山羊瘤胃液的体外批次培养研究了酵母培养物对混合瘤胃微生物体外发酵的影响。发酵底物精粗比为7:3,酵母培养物添加量为0、0.75 g/L1、.5 g/L2、.25 g/L。结果表明,发酵各时间点,各处理组的pH值较对照组均显著降低(P<0.05)。与对照组相比,发酵24 h、36 h4、8 h时,各处理组氨氮浓度显著降低(P<0.05),2.25 g/L处理组的TVFA浓度显著升高(P<0.05)。各时间点,三个处理组的乙酸浓度与对照组相比差异均不显著,而发酵24 h4、8 h时,三个处理组的丙酸浓度均高于对照组(P<0.05)。发酵24 h、48 h时,1.50 g/L处理组的乙丙比较对照组显著降低(P<0.05)。在整个发酵期内,1.50 g/L、2.25 g/L处理组的累积产气量均显著高于对照组(P<0.05),各组干物质消失率无显著差异(P>0.05)。结果显示,酵母培养物能够显著提高瘤胃VFA浓度,降低乙酸/丙酸比例,改变瘤胃发酵类型。 相似文献
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采用山羊瘤胃液的体外批次培养研究了酵母培养物对混合瘤胃微生物体外发酵的影响。发酵底物精粗比为7:3,酵母培养物添加量为0、0.75g/L、1.5g/L、2.25g/L。结果表明,发酵各时间点,各处理组的pH值较对照组均显著降低(P〈0.05)。与对照组相比,发酵24h、36h、48h时,各处理组氨氮浓度显著降低(P〈0.05),2.25g/L处理组的TVFA浓度显著升高(P〈0.05)。各时间点,三个处理组的乙酸浓度与对照组相比差异均不显著,而发酵24h、48h时,三个处理组的丙酸浓度均高于对照组(P〈0.05)。发酵24h、48h时,1.50g/L处理组的乙丙比较对照组显著降低(P〈0.05)。在整个发酵期内,1.50g/L、2.25g/L处理组的累积产气量均显著高于对照组(P〈0.05),各组干物质消失率无显著差异(P〉0.05)。结果显示,酵母培养物能够显著提高瘤胃VFA浓度,降低乙酸/丙酸比例,改变瘤胃发酵类型。 相似文献
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用体内,体外法研究纤维酶,酵母培养物对瘤胃发酵的影响 总被引:5,自引:0,他引:5
用2个试验研究添加酵母培养物(YC)商品用纤维素酶(CE)对稻草型日粮在瘤胃内发酵的影响,试验1采用人工瘤胃模拟装置,基础日粮相同,试验组分别添加YC1g/dCE1.4g/d,YC,CE能增加发酵程度,使pH值有所下降(P〉0.05),YC使发酵体系中NH3-N的平均含量提高54.31%(P〈0.01),使总N平均含量提高23.70%(P〈0.05);YC,CE对发酵系统中微生物蛋白(MCP)日粮 相似文献
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选择3只安装有永久性瘤胃瘘管的内蒙古白绒山羊羯羊作为瘤胃液供体,通过体外批次培养技术进行酵母培养物添加量的筛选试验。分别以0、0.5%、1.0%、1.5%、2.0%、2.5%和3.0%水平在以玉米-豆粕-青干草型日粮为底物的培养液中添加酵母培养物,在6、9、12、15、18、21h和24h7个培养时间点测定培养液产气量、pH值、NH3-N浓度、挥发性脂肪酸和菌体蛋白浓度,并应用多项指标综合指数(MFAEI)进行评定。结果表明:在日粮精粗比为2∶8的底物中添加2.0%的酵母培养物比较适宜。 相似文献
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高精料日粮条件下酵母培养物对瘤胃细菌体外发酵的影响 总被引:1,自引:0,他引:1
本试验研究添加不同活性和不同浓度的酵母培养物在高精料日粮的发酵体系中对混合瘤胃细菌发酵的影响.采用二因子设计,进行体外厌氧发酵,发酵结束采集样品,测定乳酸、挥发性脂肪酸及酶活浓度.结果表明,发酵24 h后各处理pH均随着酵母培养物添加量的增加而升高.活性组各处理乳酸浓度均显著低于灭活组(P<0.05),灭活组与活性组中各处理之间淀粉酶酶活均差异显著(P<0.05),且各处理均随着酵母培养物添加量的增加而逐渐降低.活性组中各处理乙丙比均显著低于灭活组,而TVFA浓度均显著高于灭活组(P<0.05),发酵系统中NH3-N浓度均随着酵母培养物添加量的增加而逐渐降低.结果提示,在高精料日粮条件下添加酵母培养物可改善瘤胃环境,改变瘤胃发酵类型. 相似文献
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瘤胃臌气是反刍类家畜临床上常见的一种病,它是牛(羊)吃了大量容易发酵的饲草料,在瘤胃内发酵,迅速产生并积聚大量气体,致使瘤胃内容积急剧增大,胃壁发生急性扩张,病畜呈现反刍和暖气困难的表现。 相似文献
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Effect of supplementation of rice bran and fumarate alone or in combination on in vitro rumen fermentation,methanogenesis and methanogens 下载免费PDF全文
Arfan Abrar Makoto Kondo Tasuku Kitamura Tomomi Ban‐Tokuda Hiroki Matsui 《Animal Science Journal》2016,87(3):398-404
This study investigated the effect of fumarate (FUM) and rice bran (RB), alone and together, on in vitro rumen fermentation, methanogenesis and methanogens. In vitro incubation was performed with six media that were either unsupplemented (control) or supplemented with 10% RB, 5 mmol/L FUM, 10% RB + 5 mmol/L FUM, 10 mmol/L FUM, or 10% RB + 10 mmol/L FUM. Methane (CH4) production, dry matter digestibility, CH4 per digested dry matter, total short‐chain fatty acid (SCFA) production, proportion of SCFA, acetate : proprionate ratio, production of NH3‐N, and population density of rumen microbes were determined. Supplementation with 10% RB + 10 mmol/L FUM yielded a 36% decrease in CH4 production compared to the control. Supplementation of FUM, in the presence or absence of RB, provided increases in total SCFA production and propionate proportion up to 61% and 31%, respectively. Total bacteria, methanogens and protozoa populations were significantly (P < 0.05) decreased with the 10% RB + 10 mmol/L FUM supplementation. The effect of anti‐methanogenesis of FUM was enhanced by the addition of RB. Notably, the CH4 production attenuation was achieved by 10% RB + 10 mmol/L FUM without reduction of digestibility or of ruminal fermentation. 相似文献
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To investigate the rumen bacterial interaction between cellulolytic Ruminococcus flavefaciens and non‐cellulolytic Selenomonas ruminantium, fiber digestibility and fermentation products were determined in defined cultures consisting of these two species. Avicel, orchardgrass hay, rice straw and alfalfa hay were used as substrates for 72 h incubation to monitor digestibility, volatile fatty acids, succinate, lactate and bacterial number. In monoculture, R. flavefaciens digested the fiber sources at 21–32%, while S. ruminantium strains did not. When R. flavefaciens was cocultured with one of three different strains (GA192, S137 and S150) of S. ruminantium, fiber digestion exceeded the value recorded by R. flavefaciens alone. In particular, cocultures with S. ruminantium S137 showed significantly higher digestibility for all the fiber sources than R. flavefaciens alone (P < 0.05). Propionate production and growth of S. ruminantium was notable in all cocultures but not in monocultures. Succinate was accumulated in monoculture of R. flavefaciens, while the accumulation was not observed in cocultures. These results indicate that R. flavefaciens provides fiber hydrolysis products to S. ruminantium as growth substrates. In addition, S. ruminantium could activate R. flavefaciens by rapidly consuming the products. Such cross‐feeding between cellulolytic and non‐cellulolytic bacteria could enhance fiber digestion, although the extent of the enhancement may depend on strain combinations. 相似文献
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The cell number of Selenomonas ruminantium (S. ruminantium) that reduces nitrate and nitrite in the rumen was usually 8–10% of the total number of S. ruminantium (an order of 106/mL). The percentage was not affected by the roughage/concentrate ratio or nitrate content of the diet in 2 weeks. However, feeding a high‐nitrate diet for 12 weeks increased the percentage. The percentage of lactate‐using S. ruminantium, such as the ssp. lactilytica, was less than 1% of the total number of S. ruminantium. No S. ruminantium was found that used formate as an electron donor for nitrate and nitrite reduction. Lactate and H2 appeared to be important for nitrate and nitrite reduction by S. ruminantium. Nitrate reduction by S. ruminantium was enhanced by the coexistence of amylolytic bacteria in a medium containing starch, and as a result, nitrite accumulation increased. Coexistence of cellulolytic bacteria facilitated the growth of S. ruminantium in a medium containing cellulose, and consequently increased nitrite reduction. In order to suppress nitrite accumulation in the rumen, it may be important to enhance fiber digestion. 相似文献
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S Sato K Ogimoto Y Nakai 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1990,52(4):711-717
Localization of bacteria and bacterial materials was investigated in calves inoculated orally with live organisms of both Bacteroides succinogenes and Selenomonas ruminantium by a immunohistological method using rabbit antiserum against the outer membrane of those organisms and by a scanning electron microscope. The intact organisms of both inoculated bacterial species were detected on the rumen wall and in the lamina propria of the forestomach, and S. ruminantium also in the lymph nodes associated with the rumen. The bacterial materials were observed inside of macrophage-like cells in the lamina propria of the forestomach and of lymphoid cells in the lymph nodes. Number of the cells in the mesenteric lymph nodes was smaller than that of the forestomach associated lymph nodes. The results suggest that the orally inoculated bacteria may act as antigenical stimulant in the mucosa of the forestomach and associated lymph nodes of calves. 相似文献
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The objective of the study was to evaluate effects of Quillaja saponaria (QS) with or without Yucca schidigera extract (YS) on in vitro ruminal fermentation and methanogenesis. The culture media consisted of 400 mL of strained rumen fluid collected from two non‐lactating Holstein cows and 400 mL of artificial saliva. The culture media was anaerobically incubated with 10 g of a mixture of concentrate and oat hay (1:1, w/w) at 39°C for 24 h. The treatments were arranged as 2 × 3 factorial design experiment consisting of three levels of QS (0, 2 or 4 mL/L) and two levels of YS (0 or 2 mL/L). Treatment interactions between QS and YS were observed for pH and ammonia N concentration. CH4 production was not modified by addition of QS but decreased (P < 0.05) when added with YS. Addition of QS with YS increased (P < 0.05) propionate concentrations. Protozoa numbers were decreased (P < 0.05) by QS addition alone or with YS. Results show that QS and YS exhibited strong antiprotozoal effects and combinations of both plant extracts may have potential as safe manipulators of ruminal fermentation. 相似文献
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Effect of ginkgo extract supplementation on in vitro rumen fermentation and bacterial profiles under different dietary conditions 下载免费PDF全文
Ginkgo extract was applied to a batch culture study and evaluated for its potential as a feed additive for ruminant animals under different forage‐to‐concentrate (F:C) ratios (1:9, 3:7, 5:5, 7:3 and 9:1). Rumen fluid was mixed with respective diet and incubated at 39°C for 24 h with and without ginkgo extract (1.6% fruit equivalent in culture). Methane production was significantly decreased by ginkgo extract, with the greatest reductions found in the 5:5 (41.9%) followed by the 7:3 ratios (36.7%). Total short chain fatty acid and ammonia levels were not affected by ginkgo extract supplementation in any of the five different diets. However, ginkgo extract increased propionate proportion and decreased acetate proportion in all dietary conditions tested. The levels of total bacteria, Ruminococcus flavefaciens, Ruminococcus albus and Fibrobacter succinogenes were decreased by ginkgo extract. The levels of Selenomonas ruminantium, Anaerovibrio lipolytica, Ruminobacter amylophilus, Succinivibrio dextrinosolvens and Megasphaera elsdenii were increased by ginkgo extract supplementation, possibly contributing to the higher propionate production. These results suggest that rumen modulation by ginkgo extract can be achieved at a wide range of F:C ratios with no adverse impact on feed digestion. Moreover, F:C ratios of 5:5 and 7:3 may be optimal when methane mitigation is expected. 相似文献
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The effect of plant oils and aspartate (ASP) on rumen fermentation in vitro. The objective of this study was to determine the effect of plant oils (rapeseed - RO, sunflower - SO; linseed - LO; 10% wt/wt) and 8 mmol sodium aspartate on rumen fermentation of a diet (250 mg) consisting of hay, barley and sugar beet molasses (60 : 30 : 10). Rumen fluid was collected from two Slovak Merino sheep fed the same diet twice daily. Mixed ruminal micro-organisms were incubated in fermentation fluid (40 ml) containing rumen fluid and McDougall's buffer (1 : 4). Incubations were carried out in batch cultures for 72 h at 39 degrees C two times in a 3-week intervals. When compared to the control, all supplemented diets (RO, SO, LO, ASP) significantly increased the pH, the mol% of propionate (LO + 8.7%; SO + 10.12%; RO + 8.65%; ASP + 5.86%) and the acetate : propionate ratio and numerically decreased methane production (SO -32.8%; LO, RO -30.08%; ASP -21.56%). Lactate production was also significantly decreased. Addition of plant oils to aspartate-treated incubations partly inhibited the decrease of n-butyrate, lactate and the increase of pH and in vitro dry matter digestibility (IVDMD) caused by ASP treatment. The effect of combined additives (RO + ASP, SO + ASP, LO + ASP) on methane production SO + ASP (-19.23%) and mol% propionate SO + ASP (+2.66%), LO + ASP (+4.28%) was less effective. All combined additives caused a significant decrease in digestibility of the given feeds. No effect of plant oils and ASP could be observed on the parameters of rumen fermentation (mainly methane and propionate). 相似文献
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《中国兽医学报》2016,(8)
选用9只装有永久性瘤胃瘘管的1周岁小尾寒羊半同胞公羊,按试验要求随机分为3组,每组3个重复,Ⅰ组为对照组,Ⅱ组投喂1.5%酵母培养物(YC1),Ⅲ组投喂2%益康XP。结果表明:Ⅱ、Ⅲ组与Ⅰ组比较均能显著提高pH值(P0.05),而Ⅱ组与Ⅲ组pH值无显著性差异;Ⅱ组NH_3-N质量浓度显著低与Ⅰ组(P0.05),Ⅲ组NH_3-N质量浓度极显著低于Ⅰ组(P0.01),显著低于Ⅱ组(P0.05);Ⅱ、Ⅲ组均有提高菌体蛋白(BCP)质量浓度的趋势(P0.05);Ⅱ、Ⅲ组均能极显著提高总酸、乙酸、丙酸浓度(P0.01)。结果提示:酵母培养物能够稳定采食后瘤胃pH值,降低瘤胃NH_3-N质量浓度,提高瘤胃TVFA浓度,有提高瘤胃BCP质量浓度的趋势。 相似文献