Double-Cropping Malaysian Prawns,Macrobrachium rosenbergii,and Red Swamp Crawfish,Procambarus clarkii

Prawns, "Macrobrachium rosenbergii," and crawfish, "Procambarus clarkii," were alternatively grown in ponds to determine if they were compatible and if total production could be increased. Brood crawfish were stocked into replicated ponds at rates of 0,60, 120 or 180 kg/ha on 18 April. Water was removed to encourage burrowing. Following this, rice was planted as forage. Post-larval prawns (0.02 g) were stocked 3 July in all ponds at 17,500/ha. Prawns in half the ponds were fed and those in the other ponds were not. Ponds were drained from 7 to 11 October. Prawn production ranged from 157 to 248 kg/ha; survival ranged from 69% to 88%, and average size ranged from 11 to 7 g. There was no significant difference (P > 0.05) between fed and non-fed treatments. The ponds were reflooded and crawfish were harvested by trapping from 15 January to 15 May. The average yield of crawfish ranged from 746 to 1,266 kg/ha. Stocking rate had no effect of crawfish yields (P > 0.25). Total yield, with prawns and crawfish combined, ranged from 1,037 to 1,237 kg/ha. Overall, prawns and crawfish were compatible in rotation. Prawns were a good size for soft shell production during months when crawfish are not available for soft shell production.     

Catch efficiency and retentive ability of commercial crawfish traps

The catch efficiency and retentive ability of 10 commercial crawfish traps were evaluated in commercial ponds. Trap designs with three entrance funnels caught significantly more crawfish than did trap designs with one or two funnels. Conversely, the retentive ability of the trap designs was inversely related to the number of entrance funnels.Traps constructed from PVC plastic-coated wire caught significantly more crawfish than identical trap designs made from galvanized wire; however, retentive ability of traps was not related to construction material. The posture (horizontal versus vertical) of identically designed traps when set in the pond did not affect the catch efficiency nor retentive ability of the traps. Traps with exposed bait caught 40–47% more crawfish than did traps with protected bait. An average of 84, 80 and 64% of marked crawfish were retained by traps after 12, 24 and 48 h, respectively.     

Evaluation of rice (Oryza sativa), volunteer vegetation,and alligatorweed (Alternanthera phyloxeroides) in various combinations as crawfish (Procambarus clarkii) forages

Four treatments of naturally occurring plants and planted rice were evaluated as forages in experimental crawfish ponds. Their relationships to biomass production, dawn dissolved oxygen, unstable water levels, and crawfish production were determined.Total standing biomass of forage at fall flooding was significantly lower (P < 0.01) for alligatorweed plus volunteer vegetation ponds compared with all other forage regimes. Average dawn dissolved oxygen during the first 5 weeks after flooding varied between 1.7 mg/l and 4.7 mg/l in ponds containing alligatorweed, compared with 1.0 mg/l and 1.5 mg/l in ponds containing other vegetation. Crawfish yields in kg/ha for ponds with stable water levels were: rice plus alligatorweed, 2852; rice only, 2652; rice plus volunteer vegetation, 2117. These forage treatments appeared to sustain the crawfish population, and stunting of crawfish at unmarketable sizes was not observed.     

Effects of Supplemental Feeding on Harvest of Red Swamp Crawfish,Procambarus clarkii,in Forage‐based Ponds

Procambarid crawfish aquaculture in the USA relies on an established forage‐based system for providing sustenance for growing crawfish. These systems may become inadequate at times for providing sufficient nourishment to support maximum growth of the population. Supplemental feeding is not routinely used because management recommendations for the cost‐effective use of supplemental feeds in ponds are not available. Because crawfish under confinement readily consume and grow well when fed single feedstuffs, such as rough rice seed and whole, raw soybeans, this study was initiated to investigate the use of these readily available low‐cost feedstuffs as supplemental feeds in aquaculture ponds. Earthen ponds (0.2 ha), constructed and managed to simulate commercial crawfish ponds, were used over three consecutive production seasons to evaluate the effects of supplemental feeding on yields and size of crawfish. Treatments for Seasons 1 and 2 consisted of (a) hull‐on rough rice seed, (b) whole raw soybean, (c) formulated 25% crude protein pellet, and (d) no supplementation and were replicated in four and three ponds for Seasons 1 and 2, respectively. Treatments for Season 3 consisted of (a) whole raw soybean and (b) no feed, with 10 replicated ponds each. Feeding frequency was established each year, and feeding rates were adjusted according to predetermined guidelines and monitoring of feeding trays. Crawfish harvesting was conducted by baited wire‐mesh traps and all harvested crawfish were subjected to a mechanical grader and sorted into three size classes. Results show that supplemental feeding, while trap‐harvesting operations are underway, can reduce yields and generate a negative economic benefit, perhaps as a result of residual feed within the pond rendering the baited trap less effective.     

Predation by Dragonfly Naiads Anax junius on Young Crawfish Procambarus clarkii

Anax junius (Odonata: Aeschnidae) predation on young crawfish, Procambarus clarkii , was measured in the laboratory. Predation rates (0.066–1.16 crawfish/day) were estimated at 25, 15, and 5 C and for two sizes of crawfish (11–20 and 21–30 mm total length). Naiad predation increased with increasing temperature and decreasing crawfish size. At 5 C, naiads had no significant effect on survival of large crawfish but did significantly reduce survival of small crawfish. At 15 and 25 C, predation rates were significantly affected by temperature and prey size. Relative naiad abundance in a 0.8 ha crawfish pond was estimated from September through May. Larger naiads (head width, >6.5 mm; total length, >30 mm) capable of preying on young crawfish were not present until after most crawfish had reached invulnerable sizes. Large naiads, as a result, may not affect crawfish production under normal management conditions.     

Effects of Added Substrate on Production of Red Swamp Crawfish, Procambarus clarkii, in Earthen Ponds without Planted Forage

The effects of addition of substrate consisting of plastic mesh fencing to experimental earthen ponds were evaluated for the culture of the red swamp crawfish, Procambarus clarkii, without planted forage. Mean daily yield (kg/ha/d), number of crawfish harvested (number/ha/d), and mean individual harvest weight were compared for ponds with and without substrate during a 2‐yr period. The surface area of added substrate was equivalent to 50% of the bottom surface area of the pond. Mean daily yield in the ponds containing substrate, based on a comparison with that of the previous year, increased by 80.2% and was principally attributed to a 66.7% increase in the number of crawfish harvested. Comparative values for the ponds without substrate were ?13.1 and ?11.7%. For the second year, the treatment‐dependent response was consistent. Mean daily yield for the substrate ponds increased by 48.8% compared to 15.0% for the ponds lacking substrate. The primary benefit of the substrate appears to be provision of refuge and three‐dimensional distribution of crawfish in the pond, thereby reducing the incidence of cannibalism and increasing the total number of crawfish harvested per hectare per day. The benefit of the substrate in a commercial production setting must be weighed against its cost and longevity as well as the conditions of the particular management scenario into which it may be incorporated.     

Effects of Water Quality,Weather and Lunar Phase on Crawfish Catch1

The relationship between water quality, weather, lunar phase and daily changes in crawfish (Proearnbums clurkii) catch per unit effort (CPUE) was determined in five experimental crawfish ponds from March through May, 1984 and 1985. Air and water temperatures, solar radiation, dissolved oxygen, wind velocity, barometric pressure, precipitation and lunar phase were recorded daily during the harvesting phase of the study. Crawfish CPUE (g/trap/24 h) was significantly correlated with water temperature, dissolved oxygen, lunar phase, barometric pressure, wind velocity and the relative density of harvestable size crawfish. Most statistically significant environmental or climatological variables explained less than 20% total daily variation in crawfish CPUE. The nine environmental variables were reduced to four factors—temperature-harvestable crawfish, cold fronts, rain showers, and lunar phase—with principal-components analysis. Eighty-five percent of the variation in daily crawfish catch was attributed to water temperature and relative density of harvestable crawfish, and 7.1% was explained by lunar phase. Rain showers and cold fronts accounted for 4.8 and 2.5% of the daily variation in crawfish CPUE, respectively. Crawfish catch increased with an increase in water temperature and relative abundance of harvestable crawfish, and with short duration rain showers. Conversely, crawfish CPUE declined with the approach of full moon and with passage of cold fronts.     

Evaluation of Trap Density and Type for Harvesting Crawfish Procambarus spp. from Small Ponds

Harvested crawfish were stocked into 0.15-ha earthen ponds in March and May at rates of 114, 227, and 455 kg/ha to evaluate the effects of trap density, 27 and 54 traps/ha, on their recapture over 14 d following 3 d of acclimation. Two-funnel, stand-up pillow traps and three-funnel pyramid traps were compared. About 39% and 55% of stocked crawfish were recovered in March and May, respectively. There was considerable growth of those crawfish. Overall catch with both trap densities was equivalent in March but the higher trap density caught 1.3 times more crawfish in May. Pyramid traps caught 1.5–1.9 times more crawfish than pillow traps.     

Growth of Crawfish Procambarus clarkii as a Function of Density and Food Resources

This study utilized enclosures (cylindrical, 5-mm wire mesh, O.5 m² bottom surface area) placed over rice-forage substrates in experimental crawfish ponds to contain crawfish under typical pond culture conditions. Juvenile Procambarus clarkii were stocked at six densities (2, 4, 6, 10, 14, and 18 crawfish/m²) for 12-wk growth trials in October and again in February. Crawfish relied solely on the detrital food system for their nutritional needs. Supplemental feed was supplied to crawfish in additional enclosures at two of the test densities (2 and 10 crawfish/m²). The commercially formulated feed (25% crude protein) was fed (2.02 g dry feed/m²) 3 d/wk (Monday, Wednesday, Friday). All treatments were replicated with six enclosures. Crawfish growth was inversely correlated to culture density. Mean final weights for crawfish feeding from the detrital-system only were 15.3, 13.8, 11.2, 7.9, 7.2, and 5.8 g for crawfish densities of 2, 4, 6, 8, 10, 14, and 18/m², respectively. Mean final weights for crawfish receiving supplemental feed were 20.7 and 12.4 g for densities of 2 and 10 crawfish/m². When compared with density as a factor influencing growth, feed influenced growth less than density abatement. Supplemental feeding improved crawfish growth in detrital systems an average of 46%, while decreasing initial density improved growth an average of 80.5%.     

Diversification of Crawfish Management Schedule1

Six 0.02 ha ponds were used to evaluate crawfish production and standing crop biomass of rice forage in ponds managed on a standard (control) and delayed (experimental) schedule over a two year production cycle. Delayed schedule followed the same sequence of events except the management activities were delayed approximately one month. Control and experimental ponds had statistically similar amounts of forage. No statistical difference was detected in crawfish production between the management schedules or between production years; however, analysis revealed a significant treatment × year interaction. Overall average crawfish production was 986 kg/ha. Variation in crawfish production is explained within the context of annual temperature variations and differences in population density.     

The Effect of Five Stocking Densities on Growth and Yield of Red Swamp Crawfish Procambarus clarkii

A 34-day study was conducted to estimate the effect of stocking density on growth, biomass, and yield of harvestable animals in red swamp crawfish, Procambarus clarkii (Girard). Juvenile crawfish were stocked at l, 2, 4, 8, and 16 crawfish per m² into plastic-lined metal pools planted with rice ( Oryza sativa ) with three replicate pools at each density. Crawfish averaged 35 ± 1.5 mm (SE) total length at the time of stocking.
Growth in total length and weight was significantly affected by density ( P < 0.01), ranging from 91.5 mm and 20.7 g for crawfish stocked at 1 per m² to 62.5 mm and 6.3 g for crawfish stocked at 16 per m². The total crawfish biomass ranged from 20.7 g per m² for crawfish stocked at 1 per m² to 88.7 g per m² at 16 per m². The portion of that biomass made up of harvestable sized animals (>75 mm total length) ranged from 100% at 1 per m² to 0.7% at 16 per m². Gross revenues per ha were projected for crawfish yields at each stocking density with and without a graded product.     

Effects of Population Density and Feeding Rate on Growth and Feed Consumption of Red Swamp Crawfish Procambarns clarkii

Wire-mesh enclosures were used in production ponds to conduct growth trials in which population density and feeding rate were evaluated in a 2 × 2 factorial arrangement for their effects on crawfish growth. Juvenile Procambarus clarkii of uniform size (0.5 g) were stocked at 2 or 20 animals/m² in experiment 1 and weighed biweekly for 12 wk. Rice forage served as the detrital base, and supplemental feed (25% crude protein) was provided at either a low (26–52 kg/ha per week) or high (104–418 kg/ha per week) rate. In experiment 2, mixed sizes of crawfish were stocked at 10/m² or 20/m² and fed the formulated feed at either 52 or 312 kg/ha per week. Feed consumption was estimated for each treatment combination. Only population density significantly affected crawfish growth. Mean weight gain and final weights were inversely proportional to density but only when total crawfish biomass exceeded 1,235 kg/ha. Growth was not significantly influenced by feeding rate, but the high feeding rate resulted in a significantly greater ratio of hepatopancreas weight to body weight and tended to decrease hepatopancreas moisture levels, indicating improved condition. Population density, feeding rate, and their interaction had significant effects on estimated feed consumption. From the simple linear regression of mean feed consumption on crawfish standing crop, it was estimated that crawfish consume about 0.1 kg of dry feed per ha per week per kg of crawfish biomass on a 5 d per week feeding schedule at mean water temperatures, ranging from 14 to 30 C.     

Pond culturing of crawfish in the Southern United States

Due to a significant increase in the farming of crawfish by pond culture methods in the southern United States this paper briefly summarises the current practice being developed there.

Crawfish are cultured in shallow, open ponds surrounded on all sides by levees. The water depth seldom exceeds 45–61 cm. Area may vary, but the most popular sizes range from 2 to 16 ha. A rotation scheme involving rice and crawfish is generally practised, with rice serving as food for the crawfish.

Good management techniques are required for maximum crawfish production. One essential element is circulation of water through the ponds to keep dissolved oxygen concentrations above 5 mg litre⁻¹. With good quality water and proper management a harvest of up to 2200 kg ha⁻¹ yr⁻¹ may be realised.     

Substitution of Soybean Protein for Fish Protein in Formulated Diets for Red Swamp Crawfish Procambarus clarkii1

A 10 week laboratory growth trial was conducted with red swamp crawfish Procambarus clarkii to determine the amount of soybean protein that could be substituted for fish protein in formulated crawfish diets without reducing growth. Crawfish received 32% crude protein, isocaloric diets in which protein was supplied by soybean (soy) meal, menhaden fish meal or an isonitrogenous mixture of soybean and fish meal calculated to provide graded levels of each protein source. Dietary protein was provided as: 1) 100% soy protein; 2) 75% soy protein: 25% fish protein; 3) 50% soy protein: 50% fish protein; 4) 25% soy protein: 75% fish protein; and 5) 100% fish protein. Crawfish fed soy protein: fish protein (SP:FP) mixtures or fish protein alone exhibited better (P < 0.05) weight gain than crawfish fed a diet containing soy protein as the only protein source. Diets containing 25% soy protein: 75% fish protein (1:3 ratio) and 50% soy protein: 50% fish protein (1:1 ratio) produced greater (P < 0.05) weight gains than a diet in which fish protein was the sole protein source. Survival, feed efficiency ratio, maturation rate, net protein and energy retention, and body composition of crawfish did not differ (P > 0.05) among diets with SP:FP ratios of 1:3, 1:1 and 3:1. However, maximum (P < 0.05) weight gain occurred in crawfish fed the 1:3 SP:FP ratio. Although crawfish fed SP:FP ratios of 1:1 and 3:1 had lower weight gains in the laboratory than crawfish fed a 1:3 SP:FP ratio, SP:FP ratios of 1:1 and 3:1 might be adequate for supplemental diets fed to pond-reared crawfish that have access to natural sources of food.     

The Economics Associated with Crawfish Production from Louisiana's Atchafalaya Basin 1

Unpredictable water levels for the Atchafalaya River resulting from man's efforts to prevent flooding in South Louisiana lead to an unstable market for Louisiana's annual crawfish production. Empirical models presented show that yields from the basin can be estimated on a monthly basis using bimonthly changes in water level, unemployment rates, and crawfish harvests from ponds. The monthly demand model for basin crawfish yielded a price flexibility of –0.059 and a corresponding price elasticity of –16.94. These empirical results support hypotheses that substitutes for crawfish are available to consumers. Uncertain yields from the basin due to the sort of water level fluctuations documented in this study suggest that investment in this area of the Louisiana crawfish industry will continue to be hampered.     

Detailed monitoring of white spot syndrome virus (WSSV) in shrimp commercial ponds in Sinaloa, Mexico by nested PCR

WSSV has caused great losses to the global shrimp industry in recent years. This virus can infect shrimps asymptomatically. However, once the clinical signs are developed, mortalities can reach 100% in 3-10 days. PCR has been extensively used to detect WSSV in a specific and sensitive manner. Nested PCR is even more sensitive than single-step PCR and had been used for the detection of WSSV in asymptomatic populations. In this work, a detailed monitoring of WSSV by nested PCR in shrimp commercial ponds in Guasave County, State of Sinaloa, Mexico, is presented. Five ponds from two different farms were monitored for growth and presence of WSSV. At the beginning of the culture, ponds from both farms showed no or very slight WSSV presence. A 3-day period of rain occurred at both farms 10 and 14 weeks of culture for farms 1 and 2, respectively. At this time, WSSV was widely distributed in the shrimp populations of farm 1 according to nested-PCR data, although no visual symptoms were observed. In ponds of farm 2, WSSV was present at low level. However, the number of PCR-positive groups was drastically increased in both farms by nested and single-step PCR. Abrupt fluctuations in temperature and salinity were documented in farm 2 after the rain, which may have contributed to the increasing of viral load in the pond's shrimp populations. Twelve days after the rain period, estimated mortalities of 80% occurred in farm 1. Nevertheless, the study ponds at farm 2 culture continued normally for three more weeks and were harvested successfully (52% and 67% of survival for ponds 1 and 2, respectively). The removal of 40% and 50% of shrimp population 2-4 days after the raining period may have contributed to the thriving of the cultures. Analyses of the presence of WSSV in individuals of both sexes indicated that there is no preference for this virus to infect male or female shrimp. Also, no differences in weight were found between WSSV infected and non-infected individual shrimps, as well as nested-PCR positive against single-step PCR positive organisms. Nested PCR is more useful to monitor shrimp cultures than single-step PCR since it allows knowing how widely distributed the virus is in asymptomatically populations.     

Molting,Mortality, and the Effects of Density in a Soft-Shell Crawfish Culture System 1

The molting and mortality patterns at a crawfish density recommended for commercial-scale soft-shell crawfish culture systems showed that, over a 116 day period, 74% of the crawfish molted, 22% died, and 4% remained in the system at the end of the study. Approximately three weeks of adaption to the system were required before molting rates of the crawfish began to increase. By the sixth week, the number of malted crawfish per day exceeded the overall mean and remained at this level until 10 days before moltable crawfish were depleted. In a second study, three density levels (3.7,4.9, and 6.1 kg crawfish/m²) were tested for 60 days. The lowest density produced significantly fewer (P < 0.05) molted crawfish. Mortality was significantly greater (P < 0.05) at the highest density compared with the lowest density. The highest density (6.1 kg crawfish/m²) showed a higher molting rate than the lower densities, but it was not significantly different (P < 0.05) from the molting rate of 4.9 kg/m².     

On the food of channel catfish stocked in farm ponds

Stomach contents of 382 channel catfish, Ictalurus punctatus (Rafinesque), ranging in length from 47 to 440 mm collected from farm ponds of Auburn University were examined. Fish in these ponds were fiven supplemental feed 6 days a week. Juvenile catfish below 100 mm fed more actively than other size groups. Channel catfish of all size groups were found to have eaten dipteran larvae and pupae more frequently than other natural food items. Dipterans contributed to a maximum of 47.7% and a minimum of 27.3% in the stomachs of 47–100 mm and 101–200 mm long fish, respectively. Supplemental feed was second in importance among other food items consumed by fish of all size groups. Other food items present in the stomachs in varying quantities were algae, molluscs, micro-crustaceans, coleopterans, trichopterans, fish remains and detritus, thus indicating the omnivorous feeding habit of channel catfish.     

Ectocommensal protozoan infestations of gills of red swamp crawfish,Procambarus clarkii (Girard), from commercial ponds

Crawfish, Procambarus clarkii (Girard), collected from commercial ponds were examined microscopically to determine the presence of ectocommensal protozoans on the gills. The relation between the incidence of gill ectocommensal protozoans and pond water quality was examined. Ninety-four percent of crawfish sampled were infested, with sixty-five percent of the infested crawfish carrying more than 100 ectocommensals per gill filament. Protozoans observed were of the genera Epistylis, Acineta, Lagenophrys, and Cothurnia, the latter being the most prevalent. Significant correlations existed between gill ectocommensal incidence and water quality variables indicative of primary productivity.     

Comparison of stocking time and density on Gulf Killifish growth in freshwater ponds

The Gulf Killifish (Fundulus grandis) is a popular baitfish and a candidate for commercial‐scale production. For inland production, ponds may need to be stocked at different times and densities to meet market demands. Therefore, in Experiment 1, juveniles were stocked into freshwater ponds at a density of 25,000 fish/ha, with two cohorts (mean total length (TL) = 3.92; 3.52 cm) stocked 1 month apart and evaluated for 12 weeks. Cohort 1 had a lower overall survival (40%) than cohort 2 (79%); with no difference between mean final TL (8.46; 8.00 cm); reaching a market size of 6 cm in 22 days and 32 days respectively. In Experiment 2, juveniles (mean TL = 3.16; 3.23 cm) were stocked into freshwater ponds at two densities (25,000 fish/ha and 50,000 fish/ha). Mean final TL was greater in the lower stocking density (8.68 cm) than the higher stocking density (8.20 cm); with no differences in survival between treatments (75; 72%) respectively. Lower stocking and higher stocking densities reached a market size of 6 cm in 29 and 31 days respectively. These results suggest that multiple cohorts can be stocked and grown to market size in one season at a stocking density of 50,000 fish/ha.