The effect of fertilizer nitrogen rate, white clover variety and closeness of cutting on herbage productivity from perennial ryegrass/white clover swards

Four varieties of white clover (small-leaved cv. Aberystwyth S184. medium-leaved cv. Grasslands Huia and large-leaved cvs Linda and Olwen) were sown at 3 kg ha^-1 together with 10 kg ha^-1 perennial ryegrass cv. Talbot. Herbage productivity was measured for three harvest years, 1979-81, over four annual rates of fertilizer N (0,120,240 and 360 kg ha^-1) and two closeness of cutting treatments (80 and 40 mm from ground level). A simulated grazing regime of six cuts per year at 3- to 6-week intervals was used.
Production of total herbage DM was increased by increasing N rate; mean annual DM production ranged from 783 1 ha^-1 with no N to 11701 ha^-1 at 360 kg ha^-1 N. Mean herbage response to N (kg DM per kg N applied) was 73,90 and 108 for the three successive N increments relative to no N. Mean white clover DM production was reduced from 4 14t ha^-1 with no N to 051 t ha^-1 at 360 kg ha^-1 N.
The large-leaved clover varieties were more productive than the small- or medium-leaved varieties at all N rates. Close cutting increased total herbage and white clover by a mean annual 16% and 31%. respectively. White clover varieties did not interact with either N rate or closeness of cutting.
It is concluded that repetitive N application over the growing season is incompatible with white clover persistence and production, even with large-leaved clover varieties or with close cutting, two factors which improved clover performance in the experimental swards.     

The productivity of four forage legumes sown alone and with each of five companion grasses

Four legumes—white clover cv. Blanca, red clover cvs Violetta (diploid) and Hungaropoly (tetraploid) and lucerne cv. Europe—were established as pure-sown swards and with each of five companion grasses: timothy cv. Timo, meadow fescue cv. Bundy, sweet brome cv. Deborah and perennial ryegrass cvs Talbot (diploid) and Barlatra (tetraploid), both ryegrasses being of 'intermediate' heading date. Two 'silage' crops and an 'aftermath grazing' crop were harvested in each of three successive years.
In the first harvest year, total herbage DM production of red clover ranged from 15·03 to 17·01 t ha^-1. White clover and lucerne swards produced considerably less at 7·12 to 11·01 t ha^-1. In the second harvest year, lucerne swards were the highest producing at 15·54 to 17·14 t ha^-1, while DM production from red clover and white clover swards ranged from 6·75 to 11·87 t ha^-1. Lucerne swards maintained their production superiority in the third year at 16·48 to 17·87 t ha^-1, while production from white clover swards ranged from 6·41 to 10·23 t ha^-1. However, red clover swards declined to 3·30 to 5·81 t ha ^-1; this above-average decline was mainly caused by the onset of red clover necrotic mosaic virus which affected all red clover plots uniformly in the second harvest year, and by winter conditions before the third harvest year. Total herbage DOM and CP yields of the swards were influenced in a similar manner to DM production.     

Cattle slurry as a source of nutrients for red clover: herbage production and clover contribution

An established sward of red clover cv. Hungaropoly sown pure received approx. 30 kg P ha^-1 and 200 kg K ha^-1 each year for 3 successive years. The P and K were applied either as cattle slurry, inorganic fertilizer or combinations of these. Treatments were applied either in spring or after the first harvest. There were a total of six treatments and these were harvested three times each year. The average yields of total herbage DM over all the treatments in the first, second and third years were 15·2, 14·2 and 14·2 t ha^-1 respectively and the various treatments had no significant effect on the overall yields.
Treatments had a significnt effect on red clover DM yields and percentage red clover in one harvest in each of the first 2 years and all three in the third year. Yields of red clover were lower and grass higher in treatments receiving cattle slurry only. On this treatment there was a total yield of 23·2 t ha^-1 red clover DM in the 3 years compared with 30·2 t ha^-1 on the inorganic fertilizer treatments. However, by applying P and K fertilizer in the spring, followed by cattle slurry after the first harvest, it was possible to maintain a high proportion of red clover in the sward and to produce yields of red clover DM similar to those on the inorganic fertilizer treatments.     

The effect of strategic nitrogen application and defoliation systems on the productivity of a perennial ryegrass/white clover sward

The productivity of a mixed sward comprising perennial ryegrass cv. Fantoom and white clover cv. Aran was measured under eight defoliation management systems and two fertilizer N rates (0 and 75 kg ha^-1) applied in spring. The defoliations involved a basic six-harvest simulated grazing system together with the interposition of silage cuts once or twice at varying times during the growing season; evaluation was made over three harvest years, 1983-85. Mean annual production of total herbage DM over the three years was 8.351 tha^-1 without N and 9.49 tha^-1 with 75 kg N ha^-1, a mean response of 15.2 kg DM per kg applied N. The responses for individual treatments occurred mainly at the first cuts, whether for simulated grazing (a mean of 12 9 kg DM) or for silage (a mean of 259 kg DM); however, this influence of spring N was not sustained at other cuts over the season. Mean annual white clover DM production was 4.19 t ha^-1 with no N and 3.32 t ha^-1 with 75 kg ha^-1 N, but the reduction due to N was not significant in any year. The mean amount of clover stolon DM present post harvest over all management systems was 1.33 t ha^-1 with no N and 1.03 t ha^-1 with 75 kg ha^-1 N. Mean annual DM production of total herbage from the six-harvest system was 8.11 t ha^-1 Compared with 8 88 t ha^-1 (a 9% increase) from the systems with one silage cut and 9.241 ha^-1 (a 14% increase) from the systems with two silage cuts. Corresponding white clover DM production was 4.02, 3 87 and 3 53 t ha^-1, respectively, and mean stolon DM amounts post harvest, 1 12,1.15 and 1-23 t ha^-1, respectively. It is concluded that grass/white clover swards are suitable for management systems which involve cutting for conservation. Spring N application did not greatly reduce white clover production in this experiment where white clover was at higher levels than are likely in farming practice and the swards were not grazed. More knowledge of spring N rates, and indeed of N application rates generally, would be advantageous in future assessment of silage cutting systems.     

Regulating the content of white clover in mixed swards using grass-suppressing herbicides

A preliminary investigation evaluated six grass-suppressing herbicides applied on two occasions in late winter to a predominantly ryegrass ley containing only 15% ground cover of white clover. Substantial increases in clover growth, estimated visually, and flower head numbers per unit area were recorded in the first summer after treatment with 2·8 kg ha^-1 carbetamide, 0·8 kg ha^-1 propyzamide and 0·6 kg ha^-1 paraquat. To achieve these increases, visual estimates suggested that spring growth of grass was reduced by 40–80%. However, grass growth recovered fully by mid-summer on the majority of the treatments.
The following year five of the herbicides were compared in a field experiment. Dry matter (DM) and nitrogen (N) assessments of the grass and legume components were made at three harvests in the first growing season and a single harvest in the second year. Carbetamide, paraquat and, especially, propyzamide increased the proportion of clover in the DM (to 89% in the case of 1·2 kg ha^-1 propyzamide); in general, using herbicides to raise clover contents above 20% lead to reductions in spring grass growth of about 70%. However, such reduction was offset by subsequent increased growth so that total annual yields were largely unaffected. The increased legume content resulted in an increased N concentration in both grass and legume components, measured in the second summer. At this time, the greatest increase in total N yield (up to 35%) was recorded from 0·6 kg ha^-1 propyzamide. Potential uses to achieve legume dominance by grass-suppression are suggested and the needs for further research are outlined.     

Lime and major nutrient fertilizers required to establish a perennial ryegrass/white clover pasture on a non-calcareous gley in the Scottish uplands

A field experiment with mixed swards of perennial ryegrass and while clover carded out in 1982–83 using small cut plots is described. With perennial ryegrass, lime slightly decreased annual dry matter (DM) production in 1982 (the sowing year) but increased it in 1983 (the first harvest year) by about 1 t ha^-1. Applications of N and P produced small increases in DM in 1982 and greater increases in 1983. In the latter season annual DM production varied from an average of 3·5 to about 10 t ha^-1 with 0 or 480 kg N ha^-1 applied in three equal-sized dressings throughout the growing season. Application of 40 kg P ha^-1 in 1982 increased DM production by about 2·5 t ha^-1 in 1983 but higher rates had little effect. Fifteen mg extractable P kg^-1 soil seemed sufficient to support levels of production normally expected from ryegrass pastures in upland Scotland, Applications of K did not affect DM production. N increased tiller weight and sward height of ryegrass; lime and P tended to increase tiller weight but this effect was not statistically significant. Leaf appearance and tiller number were not affected by treatments.
The white clover content of the pasture was decreased 10-fold by application of N and increased by lime and P (1·45 and 1·46-fold. respectively). The DM response to P was most apparent in limed soil and was also affected by the siting of the plots in the experimental area. Effects of lime and P on growth of white clover were to increase the number of stolon growing points and root nodule numbers per unit area.
The results emphasize the importance of lime and P fertilizer for establishment and growth of pasture in this soil and the differences between white clover and ryegrass in their responses to these.     

Effect of fertilizer nitrogen on six-year-old red clover/perennial grass swards

Three diploid red clover cultivars—Sabtoron, Violetta and Essex—and three tetraploid, Hungaropoly, Teroba and Red Head, were sown separately in pure culture and with each of three companion grasses: timothy (Aberystwyth S48), tall fescue (Aberystwyth S170) and perennial ryegrass (Aberystwyth S24).
The effects of fertilizer N on yield and on clover/grass ratio over a 2-year period (seventh and eighth harvest years) subsequent to 6 harvest years during which no N fertilizer was applied were investigated. The data for productivity and persistence have already been published (McBratney, 1981; 1984).
Application of fertilizer N increased DM yields in the eighth year. In this year, the highest yield, 11·9t ha^-1, averaged over the six clover cultivars, was given in association with tall fescue. Tall fescue contributed 90% of this yield. Clover content continued to decrease in all swards but the decrease was greatest in the swards receiving fertilizer N. The yield of clover DM averaged over the six cultivars under N treatment declined from 5·6t ha^-1 in the seventh year to only 0·4t ha^-1 in the eighth year.
The results from this trial demonstrate the potential of red clover sown either pure or in mixture with a suitable perennial grass, to maintain high output of quality herbage over a 6-year period without the aid of fertilizer N. They further demonstrate that following decline in red clover content, both herbage yield and quality may be restored by the application of N fertilizer, particularly where the clover was seeded with a highly productive companion grass.     

Assessment of contrasting perennial ryegrasses, with and without white clover, under continuous sheep stocking in the uplands. 5. Herbage production, quality and intake in years 4–6

Herbage characteristics were studied over years 4–6 (1988–90) in three perennial ryegrass ( Lolium perenne L.) varieties as grass-only (200 kg N ha^-1) and grass/clover ( Trifolium repens L.) swards which received 75kg N ha^-1 in 1988 and 0kg N ha^-1 in 1989 and 1990 when continuously stocked with sheep. Mean total annual herbage production of Aurora, a very early flowering variety, was 11% more than that of late-flowering Aberystwyth S23 due to 21% higher growth as grass/clover pasture. The grass/clover sward of Meltra, a tetraploid late-flowering variety, out-yielded S23/clover by 17%. Herbage production of grass/clover was 86% of that of grass only in 1988 but only 54% of the grass-only swards averaged for 1989 and 1990.
In vitro organic matter digestibility (OMD) of Meltra was 38g kg^-1 OM and 27g kg^-1 OM higher than that of S23 and Aurora respectively. OMD of grass/clover was 15g kg^-1 OM higher than that of grass only during the post-weaning period. Herbage intake was positively correlated with OMD of herbage.
The herbage attributes were related to lamb performance reported previously. Lamb output was positively correlated with intake of digestible organic matter.
Differences between the three varieties in herbage characteristics were greater as grass/clover than as grass-only swards, reflecting their compatibility with white clover. In this respect Meltra was the best and S23 the poorest variety.     

The effect of height and frequency of mowing on the yield and composition of perennial ryegrass—white clover swards in the autumn to spring period

Ninety-six plots (3 × 2 m) of well-established perennial rye grass/white clover pasture were mown to heights of 2·7 (Low) or 3·96 (High) cm (rising plate meter) at 14-, 28-, 84- or 112-d intervals in autumn-winter. A 7-, 14- and 28-d mowing interval was superimposed in spring on each autumn–winter mowing interval treatment with the low and high mowing heights altered to 2·92 and 4·80 cm, respectively.
With the low cutting height, accumulated herbage DM was more than doubled (1806 ± 79 kg DM ha^-1) compared to a 'high' (754 ± 49 kg DM ha^-1) cutting height in autumn–winter and this was due to increased harvesting efficiency rather than growth as estimated by leaf extension. Although defoliation interval had no effect on DM yield, the grass component increased and clover decreased. The composition effect carried over into spring. On average, 3·5 tillers were produced over winter for each ryegrass tiller present in autumn and tiller densities were higher in spring. Tillers produced over autumn–winter contributed more than 60% of ryegrass growth by early spring.
In early spring (16–30 September), the low cutting height increased herbage DM yield, in mid-spring (1–14 October) it reduced DM yields particularly in combination with short defoliation intervals, while in late spring (14 October to 11 November) cutting height had no effect on DM yields.
Over the entire spring period there was a very marked effect of defoliation interval on DM yields.     

Silage and milk production: studies with diets containing white clover silage

In three separate feeding experiments using a total of twenty-six individually-housed Ayrshire cows, three wilted silages made from Blanca white clover were offered ad libitum with either different supplements or different proportions of grass silage. The clover silages contained 680 g white clover kg⁻¹ on a DM basis, and had a mean DM concentration of 263 g kg⁻¹ with 231 g CP kg⁻¹ DM and 91 g ammonia-N kg⁻¹ N. The pH values averaged 4·16 and the DOMD concentrations 611 g kg⁻¹. In experiment 1 the daily intake of clover silage given alone was 15.2 kg DM per cow, i.e. 30·1 g kg⁻¹ live weight, and decreased by 0·76 kg DM kg⁻¹ barley DM and by 0·66 kg DM kg⁻¹barley plus soybean meal DM when these feeds were offered as supplements. Milk yield and fat concentration were higher on the supplement treatments than on the clover silage-only treatment. In experiments 2 and 3 the intakes of silage and total DM increased as the weight of clover in the diet increased from 0 to 700 g kg⁻¹ with parallel increases in milk yield. The effects on milk composition were small and generally non-significant. Although white clover silages with excellent fermentations were made, it is concluded that the main role of white clover in a silage system will be in mixed swards with grass to reduce the input of fertilizer N and to increase the voluntary intake of silage.     

The effects of nitrogen fertilizer applied in spring on swards of ryegrass sown with four cultivars of white clover

Nitrogen fertilizer was applied at 0, 30,60 and 90 kg N ha^-1 in March 1976, 1977 and 1978 to plots containing perennial ryegrass cv. Barlenna sown either alone or with white clover cv. Blanca, Sabeda, Olwen and S100 in four replicated blocks.
Dry matter (DM) yield of all swards responded positively to N with the response being highest in the no-clover swards in two of the three years. Annual DM yields were lower in the no-clover than in the mixed swards at any given N level. The higher levels of N reduced DM yield in mixed swards at some harvests in midsummer.
Swards of S100 were consistently among the lower yielding mixed swards, whereas the other three clover cultivars varied in their relative yields. There were no N x cultivar interactions for white clover yields; irrespective of cultivar, N application reduced annual white clover yield by similar amounts.
It is concluded that it is beneficial to apply moderate amounts of N fertilizer to mixed swards in spring and that under these conditions the yield differences between swards including medium-large and smaller leaved white clover cultivars are similar to differences between the inherent yield potential of these cultivars in mixed swards receiving no N.     

The effect of harvesting system on nutrient losses during silage making. 1. Field losses

An experiment was carried out during 1982 in which the effects of three differing harvesting systems on the field losses occurring during the conservation of grass as silage were examined. The treatments were either (a) harvesting herbage directly by means of a flail harvester (unwilted flail, UF), or (b) pre-cu ing of herbage with a rotary drum mower and lifting using a precision-chop forage harvester with wilting (WP) and without wilting (UP). Herbage was harvested on 26 May, 21 July and 7 September 1982. The mean yields of herbage produced from plots harvested by the three systems were not significantly different, with dry ma er (DM) yields of 12470, 12300 and 12230 kg ha^-1 for the UF, UP and WP treatments respectively. However, field losses with the UP and WP treatments were greater than with the UF treatment (P<0·01). As a result the yields of herbage ensiled with the UP and WP treatments, 11690 and 11320 kg DM ha^-1 respectively, were significantly lower than with the UF treatment, 12710 kg DM ha^-1 (P <0·05). Wilting of first cut herbage for a period of 72 h resulted in a significant reduction (P<0·05) in digestible organic ma er (DOM) concentration (determined in vitro), whereas small increases in DOM concentration were observed following wilting of second and third harvest material.
It is concluded that, even under favourable weather conditions, both pre-cutting and wilting of herbage prior to harvesting can result in considerable losses of nutrients in the field.     

The influence of surface and sub-surface application methods for pig slurry on herbage yields and nitrogen recovery

Experiments were conducted on a grassland site at Wrest Park, Silsoe, Bedfordshire between 1987 and 1989, to compare herbage yields from slurry applied by deep and shallow injection, low trajectory and conventional vacuum tanker methods. Slurry application rates for all spreaders were calibrated at 86 ± 5 t ha^-1, an equivalent of c. 200 kg NH₄⁺-N ha^-1 applied in autumn or in spring.
As expected, herbage yields following spring applications were higher than from autumn applications, with average mineral fertilizer equivalents of 122 and 89 kg N ha^-1 respectively. Yields from the conventional and low trajectory spreaders showed no consistent differences. However, in both years, first cut yields from plots were significantly lower ( P < 0·05) where slurry had been injected than where surface applications had been used by an average of 0·7 t DM ha^-1. Subsequent cuts in 1988 demonstrated higher residual effects from injection so that annual total yields were similar from all slurry applications irrespective of spreader type.
Analysis of N content revealed high N levels in herbage from deep injection plots. Mean concentrations of N in the herbage, expressed as a percentage of the dry matter, were 1·43 for surface treatments and 1·79 for deep injection in 1988, and 1·84 for surface treatments, 2·13 for shallow injection and 2·68 for deep injection in 1989.     

Effect of regrowth interval on the productivity of swards defoliated by cutting and grazing

Over three grazing seasons (1984-1986) a sward of perennial ryegrass, cv. Talbot, which received a total of 336 kg N ha^-1 each season, was cut or grazed with ewes at 3- or 4-week intervals on a rotational basis.
Sward productivity was higher under cutting than under grazing irrespective of the interval between defoliations. Under cutting, mean herbage organic matter (OM) yields over both intervals were 8·66, 9·62 and 8·17 t ha-¹ in 1984, 1985 and 1986 respectively while under grazing the corresponding yields were 7·65, 8·63 and 7·50 t ha^-1. The mean annual yield of herbage defoliated at 3-week intervals was 7·50, 8·64 and 7 ·20 t OM ha^-1 compared with 8·80, 9·60 and 8·46 t OM ha^-1 for swards defoliated at 4-week intervals in the three years respectively.
The nitrogen (N) content of both the available and the residual herbage was consistently higher under grazing than under cutting. Available herbage contained 31·3 and 27·7 g N kg OM^-1 and residual herbage 26·1 and 22·7 g N kg OM^-1 under grazing and cutting respectively.
The mean yield of N under cutting was 284 kg ha^-1 compared with 304 kg ha^-1 under grazing. Defoliation interval had no effect on N yield, the overall mean yield being 294 kg ha^-1 under both 3- and 4-week defoliation intervals. The effect of the treatments on tiller population was slight and inconclusive.
The process of grazing reduced yield probably as a result of damage to the sward through trampling; the positive effect of excretal N on yield was minimal on account of the short grazing periods.     

Further studies on the effect of phosphate fertilizer and propyzamide on the growth of white clover slot-seeded into permanent pasture

Assessments were made of the clover yield and clover content between early June and late July 1984 of white clover in a permanent pasture which had been slot-seeded in spring 1982.
Past reserves of phosphate or application of propyzamide alone only slightly increased clover dry matter yield but together they increased it from 13 to 785 kg dry matter ha^-1 and gave 31% clover in the total herbage yield. Applications of P₂O₅ during the experiment, alone or together with past reserves of phosphate, Iased clover yield from 13 to 242 kg dry matter ha^-1 and the proportion of clover from 1 to 10%. However, highest yields (1282 kg) and clover content (37%) were attained when P₂O₅ and propyzamide were applied to treatments with past reserves of phosphate.
These results confirmed the trends evident during the second year and also indicated a potential role for grass-suppressants in increasing management options during the early years after slot-seeding.     

Supplementary feeding of forage to grazing dairy cows, 3. The effect of three daytime stocking rates on the performance of cows offered grass silage overnight

Over a 24-week period, three groups of dairy cows were continuously stocked at 8, 10 or 12 cows ha^-1 between morning and afternoon milkings, and overnight were housed and offered grass silage ad libitum. Due to a prolonged drought, sward heights only averaged 4·1 cm.
The increase in daytime stocking rate led to a decline in herbage intake, and increases in silage intake. At the highest stocking rate (12 cows ha^-1), the silage intake failed to compensate for the reduced herbage intake. Consequently the total dry matter and estimated metabolizable energy intakes were lower than for the 8 and 10 cows ha^-1 treatments. Milk yields and milk composition were not significantly affected by treatment but the 12 cows ha^-1 stocking rate gave the lowest milk and milk solids yields.
The utilized metabolizable energy (UME) on the grazed swards was greatest for the 10 cows ha^-1 treatment. The sward cut to provide the silage had a UME level (GJ ha^-1) 32% greater on average than the grazed swards during the same growth period. The total areas utilized for grazing and silage production for 8, 10 and 12 cows ha^-1 were 0·240, 0·224 and 0·215 ha respectively. Fat and protein yields per unit area were greatest for the 10 cows ha^-1 group.     

Organic and inorganic fertilization compared in a natural Mediterranean grassland

The effect of goat slurry on species composition and herbage production as compared to inorganic fertilizer was studied in a natural grassland dominated by warm and cool season perennial grasses in Macedonia, Greece. Goat slurry was applied in early spring every 1 or 2 years at a rate of 40 t ha^-1 being equivalent to about 160 kg N ha^-1 and 100 kg, P₂O₅ ha^-1. Inorganic fertilizer was also broadcast in early spring at a rate of 80 kg N ha^-1 and 100 kg P₂O₅ ha^-1 every 2 years. Measurements of the basal cover of the dominant species or groups of species and herbage yields were taken for 6 years at the end of the growing period in June, while in the seventh year the experiment was grazed with goats. It was found that goat slurry improved species composition more than the inorganic fertilizer by depressing the less palatable warm season grasses and favouring the subdominant cool season grasses and legumes. Goat slurry significantly increased herbage yields in most of the years. It is suggested that the optimum rate of goat slurry is 40 t ha^-1 every 2 years as this encourages plants most preferred by goats.     

The comparative performance of Holcus lanatus and Lolium perenne under sheep grazing in the Scottish uplands

An experiment was conducted over 3 years (1983-85) to assess the performance of Holcus lanatus German Commercial and cv. Massey Basyn compared with Lolium perenne cv. Perma on a gley soil under sheep grazing in the Scottish uplands. All grasses were sown together with Trifolium repens cv. Grasslands Huia. The swards were rotationally grazed at similar herbage allowances. During the first harvest year, the swards were grazed hard to a low mass (500 kg DM ha⁻¹). In the second harvest year, post-grazing herbage masses of 500 and 1000 kg DM ha⁻¹ were compared.
Perma ryegrass had a higher level of herbage production than both H. lanatus cultivars in the first and second harvest years after sowing and hence had a greater number of sheep grazing days. The ryegrass sward consisted of 25% more green sown grass (85 cf. 60%) but 12% less white clover (4 cf. 16%) compared with both H. lanatus cultivars in the establishment and first harvest years. The persistence of all three grasses was poor although ryegrass had a higher presence (36%) than either Massey Basyn (22%) or German Commercial (13%) at the end of the. second harvest year.
At a similar herbage allowance, there were no significant differences in the herbage intake and liveweight gain of sheep. Ryegrass had a higher organic matter digestibility and lower neutral and acid detergent fibre and lignin contents than either of the H. lanatus cultivars.
In the second harvest year, although herbage production was greater at the higher herbage mass, there was no difference in the proportion of sown grass.
It was concluded that ryegrass is a superior grass to H. lanatus on upland soils with high N status, moderate P status and a high pH.     

The effect of companion perennial ryegrass cultivars on red clover productivity when timing of the first cut is varied

Replicated plots of Hungaropoly red clover were sown on a sterilized area in May 1975 alone (seed rate 11 kg ha^-1) or with one of six cultivars of perennial ryegrass (seed rate 3·5 kg ha^-1) viz. Cropper and S24 (early heading), Barlenna and Hora (medium heading) and Melle and Perma (late heading). In 1976 and 1977 primary growth was cut at one of four dates ranging from mid-May to mid-June and thereafter plots were harvested twice each year.
Varying the time of first cut did not have a significant effect on total dry matter (DM) yield in either year despite differences in means of cutting treatments on annual red clover yields of the order of 6–9%.
In some companion grass treatments total DM yield in 1976 was increased and total red clover yield and percentage red clover contribution were reduced relative to swards sown only with red clover. In 1977 a similar but non-significant trend was found. Swards containing early ryegrasses had higher total herbage DM yields but lower red clover yields and contents than all other swards at the first harvest in both years.
Delay in date of taking the first harvest in 1976 reduced DM digestibility in the first cut and increased it in the second in both years.
It is suggested that by cutting early and increasing the number of harvests from three to four per year, differences in the content of red clover between the first and second cut might be reduced, and it is concluded that more benefit is derived from red clover when medium or late heading ryegrasses are used as companion grasses.     

Seasonal changes in the crude protein concentration of mixed swards of white clover/perennial ryegrass grown without fertilizer N in an organic farming system in the United Kingdom

Changes in the crude protein (CP) concentration of white clover and perennial ryegrass herbage from a mixed sward were determined on six sampling dates from May to October in each of 2 years. The swards were grown without fertilizer N in an organic farming system and continuously grazed by dairy cows during the grazing season. The annual mean contents of white clover in the dry matter (DM) of the sward were 272·3 and 307·0 g kg⁻¹ in Years 1 and 2. The mean CP concentrations of the white clover and perennial ryegrass herbage were 251·6 and 151·9 g kg⁻¹ DM in Year 1 and 271·9 and 174·0 g kg⁻¹ DM in Year 2 respectively. The CP concentration of the white clover increased significantly during the grazing season from 220·0 to 284·1 g kg⁻¹ DM in Year 1 and from 269·0 to 315·5 g kg⁻¹ DM in Year 2. In the perennial ryegrass herbage the CP concentration increased from 112·2 to 172·6 g kg⁻¹ DM in Year 1 and from 142·7 to 239·5 g kg⁻¹ DM in Year 2. The rate of increase during the season in the CP concentration of the perennial ryegrass herbage was similar to the rate of increase recorded in the white clover herbage.