The effect of strategic nitrogen application and defoliation systems on the productivity of a perennial ryegrass/white clover sward

The productivity of a mixed sward comprising perennial ryegrass cv. Fantoom and white clover cv. Aran was measured under eight defoliation management systems and two fertilizer N rates (0 and 75 kg ha^-1) applied in spring. The defoliations involved a basic six-harvest simulated grazing system together with the interposition of silage cuts once or twice at varying times during the growing season; evaluation was made over three harvest years, 1983-85. Mean annual production of total herbage DM over the three years was 8.351 tha^-1 without N and 9.49 tha^-1 with 75 kg N ha^-1, a mean response of 15.2 kg DM per kg applied N. The responses for individual treatments occurred mainly at the first cuts, whether for simulated grazing (a mean of 12 9 kg DM) or for silage (a mean of 259 kg DM); however, this influence of spring N was not sustained at other cuts over the season. Mean annual white clover DM production was 4.19 t ha^-1 with no N and 3.32 t ha^-1 with 75 kg ha^-1 N, but the reduction due to N was not significant in any year. The mean amount of clover stolon DM present post harvest over all management systems was 1.33 t ha^-1 with no N and 1.03 t ha^-1 with 75 kg ha^-1 N. Mean annual DM production of total herbage from the six-harvest system was 8.11 t ha^-1 Compared with 8 88 t ha^-1 (a 9% increase) from the systems with one silage cut and 9.241 ha^-1 (a 14% increase) from the systems with two silage cuts. Corresponding white clover DM production was 4.02, 3 87 and 3 53 t ha^-1, respectively, and mean stolon DM amounts post harvest, 1 12,1.15 and 1-23 t ha^-1, respectively. It is concluded that grass/white clover swards are suitable for management systems which involve cutting for conservation. Spring N application did not greatly reduce white clover production in this experiment where white clover was at higher levels than are likely in farming practice and the swards were not grazed. More knowledge of spring N rates, and indeed of N application rates generally, would be advantageous in future assessment of silage cutting systems.     

The effect of strategic use of fertilizer nitrogen in spring and/or autumn on the productivity of a perennial ryegrass/white clover sward

The productivity of a mixed sward, comprising perennial ryegrass cvs Barlano and Bastion and white clover cvs Donna and Aran, was measured under sixteen fertilizer N treatments. These involved 0.25, 50 and 75 kg N ha^-1 in spring only, in autumn only and in all combinations of spring N and autumn N. A simulated grazing regime of six cuts annually at 3- to 6-week intervals was imposed. Increasing rates of total N application increased total herbage DM regardless of application pattern. Yield response was greater with N applied in the spring, and total herbage DM was higher with high spring N-low autumn N than the reverse. Mean yield responses at the first harvest to 25, 50 and 75 kg ha^-1 N in spring were 13.6, 10.8 and 11.6 kg DM per kg N. Corresponding responses at the final harvest to N rates in the autumn were 7.2, 5.8 and 6.8 kg DM per kg N. Responses were similar at these times for treatments receiving combined spring and autumn N. Over all treatments, mean annual production of total herbage was between 7.08 t ha^-1 DM with no N and 8.19 t ha^-1 with 75 kg ha^-1 N in both spring and autumn. Owing to drought, mean production in year 2 fell by 32% compared with year 1. White clover production fell progressively with increasing N application. Treatments with spring-applied N gave the most marked decrease. White clover was more markedly depressed than the associated grass by the drought in the second year. The mean reductions in white clover content were 0.17, 0.07 and 0.12 percentage units per kg applied N for spring N, autumn N and combinations. Autumn N use depressed white clover less than spring N but the yield response of grass was less. It is concluded that any applied N adversely affects white clover performance to some degree. Where management factors are unfavourable to white clover even strategic N use may not be wise. Instead, it is suggested that a ‘dual-sward’ approach be adopted in practice, namely, grass/white clover swards with no N. and complementary grass swards receiving optimum applied N to give better production at times when grass/white clover swards are relatively less productive.     

The effect of fertilizer nitrogen rate, white clover variety and closeness of cutting on herbage productivity from perennial ryegrass/white clover swards

Four varieties of white clover (small-leaved cv. Aberystwyth S184. medium-leaved cv. Grasslands Huia and large-leaved cvs Linda and Olwen) were sown at 3 kg ha^-1 together with 10 kg ha^-1 perennial ryegrass cv. Talbot. Herbage productivity was measured for three harvest years, 1979-81, over four annual rates of fertilizer N (0,120,240 and 360 kg ha^-1) and two closeness of cutting treatments (80 and 40 mm from ground level). A simulated grazing regime of six cuts per year at 3- to 6-week intervals was used.
Production of total herbage DM was increased by increasing N rate; mean annual DM production ranged from 783 1 ha^-1 with no N to 11701 ha^-1 at 360 kg ha^-1 N. Mean herbage response to N (kg DM per kg N applied) was 73,90 and 108 for the three successive N increments relative to no N. Mean white clover DM production was reduced from 4 14t ha^-1 with no N to 051 t ha^-1 at 360 kg ha^-1 N.
The large-leaved clover varieties were more productive than the small- or medium-leaved varieties at all N rates. Close cutting increased total herbage and white clover by a mean annual 16% and 31%. respectively. White clover varieties did not interact with either N rate or closeness of cutting.
It is concluded that repetitive N application over the growing season is incompatible with white clover persistence and production, even with large-leaved clover varieties or with close cutting, two factors which improved clover performance in the experimental swards.     

Effect of cultivar and seed rate of perennial ryegrass and strategic fertilizer nitrogen on the productivity of grass/white clover swards

Four cultivars of perennial ryegrass (intermediate diploid cv. Talbot and tetraploid cv. Barlatra, and late diploid cv. Parcour and tetraploid cv. Petra) were each sown at 10,20 and 30 kg ha^-1, all with 3 kg ha^-1 of white clover cv. Donna. Herbage productivity was measured over 3 harvest years, 1982–84. under two annual rates of fertilizer N (0 and 150 kg ha^-1); the 150 kg ha^-1 rate was split equally between March and August applications. Fertilizer N increased total herbage DM production; the 3-year means for the 0 and 150 kg ha^-1 N rates were 8·04 and 8·91 t ha^-1, respectively. In successive years, total herbage responses to N (kg DM (kg N applied)^-1) were 6·6, 35 and 72 (overall mean, 58). Mean white clover DM production over the 3 years was reduced from 4·48 t ha^-1 at nil N to 2·82 t ha^-1 at the 150 kg ha^-1 rate, a fall of 37%. Grass seed rate did not influence total herbage production or white clover performance. The two intermediate perennial ryegrass cultivars had a marginal advantage in total herbage production over the two late cultivars, but white clover content and production were higher with tetraploids than diploids. It is concluded that the value of increased herbage production from strategic use of fertilizer N has to be weighed against its depressive effect on white clover performance; application of 75 kg ha ha^-1 N in both spring and autumn was excessively high if maintenance of a good white clover content in the sward is an objective. There is considerable flexibility in the grass: clover seed ratio in seeds mixtures. Modern highly-productive perennial ryegrass varieties do not differ substantially in compatibility with white clover but tetraploids permit better clover performance than diploids.     

Effects of spring fertilizer nitrogen and sward height on production from perennial ryegrass/white clover swards grazed by beef cattle

An experiment was carried out to compare the effects of two compressed sward height treatments, each at two fertilizer nitrogen (N) treatments (0 and 50 kg ha⁻¹ in spring), on the date of turnout and liveweight gain of steers grazing a perennial ryegrass/white clover ( Lolium perenne/Trifolium repens ) sward sited on clay loam in south-west England. The sward height treatments were 6 cm all season, and 4 cm in the spring rising to 6 cm in June; these were maintained using continuously variable stocking with Hereford × Friesian steers. Cattle were turned out on average 11·5 days earlier on the 4-cm sward height compared with the 6-cm sward height treatment. Liveweight gain early in the season was lower on the 4-cm swards than on the 6-cm swards. Liveweight gain ha⁻¹ over the whole season was similar for the two sward height treatments. Fertilizer N did not affect turnout date or liveweight gain.     

The influence of fertilizer nitrogen, white clover content and environmental factors on the nitrate content of perennial ryegrass and ryegrass/white clover swards

In a field experiment carried out over 3 years, the nitrate content of herbage from perennial ryegrass (Lolium perenne) swards increased exponentially with nitrogen application rate, but herbage nitrate content appeared to reach potentially dangerous concentrations only when nitrogen application rates were greater than those needed to stimulate dry-matter production. Thus, on average over all the harvests, maximum yield could be obtained with annual application rates of 400 kg N ha^–1 (six applications of 67 kg N ha^–1) for perennial ryegrass and 300 kg N ha^–1 (six applications of 50 kg N ha^–1) for perennial ryegrass/white clover (Trifolium repens) swards, whereas the mean nitrate concentrations were 3340 and 2929 mg NO₃ kg^–1 dry matter (DM) respectively. Nitrate content, however, varied considerably from harvest to harvest, reaching maxima of 9345 mg NO₃ kg^–1 DM at 400 kg N ha^–1 for perennial ryegrass and 6255 mg NO₃ kg^–1 DM at 300 kg N ha^–1 for perennial ryegrass/white clover. The nitrate content of herbage from perennial ryegrass/white clover swards was always greater than that of perennial ryegrass swards receiving the same rate of nitrogen application, even though in the herbage from the mixed sward the nitrate content of white clover was usually less than half that of the perennial ryegrass component. The physical environment did not have a clearly interpretable effect on nitrate content, although herbage harvested in May had a much lower nitrate content than that harvested at any other time of the season. It was not possible to find a single multiple regression equation relating herbage nitrate content to nitrogen application and to other environmental variables that explained more than 60% of the variance in herbage nitrate, but it is suggested that, by reducing the later-season nitrogen applications from 67 to 50 and finally to 33 kg N ha^–1 for perennial ryegrass and from 50 to 33 kg N ha^–1 for perennial ryegrass/white clover, it would be possible to achieve over 90% of the maximum yield while reducing average nitrate content to <40% of that at maximum yield, with no samples containing more than 2300 mg NO₃ kg ^–1 DM.     

The effects of sward height and nitrogen fertilizer application on changes in sward composition, white clover growth and the stock carrying capacity of an upland perennial ryegrass/white clover sward grazed by sheep for four years

Perennial ryegrass/white clover pastures grazed by sheep and receiving either no fertilizer N (No) or 120 kg N ha^?1 year^?1 (N₀) were maintained with surface heights of 2·5, 3·5 and 5·0 cm for over four years. The treatments were replicated. The white clover (WC) population was greatest in the N₀treatment, and declined during the study. Between-year variation in WC was negatively related to rainfall and positively related to temperature, WC as a proportion of the total plant population decreased during the summer in the Nl treatment. The perennial ryegrass (PRG) population was greater in the Nl treatment, declined during the study and both within and between years was positively related to temperature. The population density of the unsown grasses was highest in the N-fertilized treatment and in the swards maintained at the lowest heights (these treatments also had the highest stocking rate); it increased during the study, within-years being positively related to temperature and between-years being positively related to rainfall. The WC stolon extension rate was largely unaffected by N fertilizer application and was greatest in the taller swards. Leaf appearance rate was unaffected by N fertilizer application and sward height; it was positively related to temperature and negatively related to rainfall. Branching rate was greater in the N₀ treatment with significant sward height effects confined to a negative relationship with local sward height within treatment plots on one occasion; it was negatively related to rainfall. The ground level red:far red light ratio was negatively related to local sward height. The total live weight of sheep carried in the No treatments was 0·7 of that in the N₁ treatments. Expected photomorphogenic responses by we were confined to stolon extension. It was concluded that on the poorly drained clay-loam soil used in this study the effects of sheep, in interaction with climatic factors, had an overriding effect on clover branching rate and the ultimate species composition.     

The effect of pesticide application on the establishment of white clover in a newly-sown ryegrass/white clover sward

In plots of perennial ryegrass and white clover, regular treatment with chlorpyrifos and methiocarb more than trebled the clover content of the sward, compared with untreated plots, in just over 2 years from sowing. Chlorpyrifos alone accounted for most of the effect, which was attributed mainly to the control of Sitona lepidus. Combined treatment increased total herbage dry matter yield by 4·24 t ha⁻¹ during this period but had little effect on grass-only plots. Methiocarb appeared to have contributed by assisting earlier establishment of clover, but otherwise had little effect until slugs, which were initially uncommon, became more abundant in the later stages of the experiment. The effect of slug control was most noticeable in a cultivar of low cyanogenic potential. This factor did not influence the effect of chlorpyrifos.     

The effect of sward height on responses of mini-swards of perennial ryegrass/white clover to slurry application

The influence of sward height at the time of slurry application on sward responses to slurry was investigated using perennial ryegrass ( Lolium perenne )/white clover ( Trifolium repens ) mini-swards under greenhouse conditions. Pig slurry, cattle slurry and a fertilizer control were applied either to: swards cut 1 d before slurry application to heights of 2, 4 or 8 cm (CH2, CH4 or CH8); or to swards cut to 4 cm with regrowth intervals of 1, 4 or 8 d before slurry application (RI1, RI4 and RI8). Scorch, smother and growth of marked clover stolons and grass tillers were monitored after slurry application. Dry-matter yields of both species were recorded over two harvests. Electrolyte leakage from leaves was used to assess leaf damage. Both slurries increased leakage, which was greatest from clover leaves, with biggest increases caused by pig slurry. Yield responses to slurry application varied with plant species and sward height. Reduced grass growth after slurry application to long swards (CH8, RI4, RI8) was probably caused by greater smother. In short swards (CH2), grass leaf growth was not depressed following slurry application but grass yields were reduced. Ammonia volatilization losses may have been greater in both long and short swards, contributing to yield reductions. Yield responses to slurry were greatest in swards cut to 4 cm, 1 d before application.     

The effects of stocking rate and nitrogen fertilizer on a perennial ryegrass-white clover sward

The effects of stocking rate and N fertilizer on a mixed sward of perennial ryegrass ( Lolium perenne) and white clover ( Trifolium repens ) set-stocked with sheep were examined. Sward production and composition, and sheep production were studied.
Increasing the stocking rate over the range 25–55 yearling sheep ha⁻¹ reduced herbage accumulation by about 40%, whether or not N fertilizer was applied. Increasing the stocking rate increased the density of ryegrass tillers, but reduced the density of clover stolons and the clover content of the swards. Applications of N fertilizer (200 kg N ha⁻¹ a⁻¹) increased herbage accumulation by about 20% but substantially reduced the clover content.
Liveweight gain per animal and per unit area were greater at the lower stocking rates where the clover content and nutritive value of the diet were greatest. Wool growth per unit area was greater at the higher stocking rates. Applications of 1M fertilizer increased liveweight gain at stocking rates above 25 sheep ha⁻¹, but had no effect on wool production at any stocking rate.
The results demonstrate that a stable and productive grass-clover association was maintained under conditions of set-stocking at around 23 yearling sheep ha⁻¹and that at this stocking rate, which appears to be about the biological optimum, there was no advantage in using N fertilizer.     

Effects of sward type and rest periods from sheep grazing on white clover presence in perennial ryegrass/white clover associations

The effects of the imposition and timing of a rest period from continuous sheep stocking, for a conservation cut, on white clover presence in forty perennial ryegrass/white clover associations were studied over two full grazing seasons. Each association consisted of one grass variety along with one white clover variety, the grasses being diploid and tetraploid ryegrasses from each of five maturity groups and the white clovers from each of four leaf size categories. The presence of white clover within each association was assessed at the beginning and end of both seasons by means of a 0–64-m² quadrat subdivided into 100 squares, each 80 mm × 80 mm, the number of squares in which any part of a white clover plant was visible being recorded. Complementary point quadrat data were also collected. Although continuous sheep stocking did not necessarily have an adverse effect on white clover presence, a July to mid-August rest period increased white clover proportions in the swards (means: unrested, 48–1; April to late May rest, 32.7; July to mid-August rest, 67.3 - s.e.d. 7.59; P < 0.05) the benefit increasing with increasing white clover leaf size. The early rest period (April to late May) reduced white clover presence and the late rest period (July to mid-August) increased white clover presence, these effects being intensified with increasing white clover leaf size (very large-leaved clover: unrested, 20.6; April to late May rest, 8.3; July to mid-August rest, 41.1 and small-leaved clover: unrested, 96.3; April to late May rest, 84.8; July to mid-August rest, 97 - s.e.d. 9.2; P <0001). Tetraploid ryegrass/white clover associations had consistently and significantly more white clover than diploid ryegrass/white clover associations of similar ryegrass maturity group (tetraploid, 53.4; diploid 44.8 - s.e.d. 2.12; P <0.001) and associations with early maturing ryegrass contained more white clover than those with late maturing ryegrasses, the effect of maturity group being greater than that of ploidy. Overall, white clover presence increased with increasing openness of grass growth habit.     

Effects of interval between harvests and application of fertilizer N in spring on the growth of perennial ryegrass in a grass/white clover sward

A perennial ryegrass/white clover sward, which had been grazed for over 2 years, was cut at 1-, 2-, 3- or 6-week intervals from 18 April to 28 November 1986. Two rates of fertilizer N application in spring, 0 and 66 kg N ha⁻¹, were compared. Perennial ryegrass growth was studied in three 6-week periods, beginning on 18 April, 18 July and 17 October. Clover growth was studied in the same three periods and described by Fisher and Wilman (1995) Grass and Forage Science , 50 , 162–171.
Applied N increased the number of ryegrass tillers m⁻², the rate of leaf extension and the weight of new leaf produced tiller⁻¹ and m⁻²per week. Increasing the interval between cuts reduced the number of ryegrass tillers m⁻² and increased the rate of leaf extension, weight tiller⁻¹and the weight of new leaf produced tiller⁻¹week⁻¹. Increasing the interval between cuts increased the weight of new ryegrass leaf produced m⁻² where N had recently been applied, but otherwise had little effect on the weight of new leaf produced m⁻². Applying N favoured the grass in competition with the clover in every respect, whereas increasing the interval between cuts only favoured the grass, compared with clover, where N had recently been applied; where N had not been applied, the ratio of ryegrass tillers to clover growing points in the sward was very little affected by the interval between cuts.     

The effect of grazing interval on the composition and productivity of a perennial ryegrass-white clover sward

A ryegrass-white clover sward was grazed by Welsh Mountain ewes with single lambs at a stocking rate of nineteen ewes per ha from April until October. Rotational grazing was practised and the treatments compared were rest periods of 3, 4 or 5 weeks. The percentage of ground covered by clover was estimated visually and quadrats were also cut to ground level to estimate the amount of clover dry matter present. A good correlation was obtained between these two estimates. Although the sward contained about 20% clover on visual assessment, the dry matter contribution was small. The amount of clover increased slightly over the season on all treatments, but the different rest periods of 21, 28 and 35 d made no significant difference to the amount of clover present. The growth rate of the lambs was also not significantly different between treatments.     

The combined use of fertilizer nitrogen and white clover on grazed ryegrass swards

A 1-year grazing experiment with dairy cows is described in which the milk yield and herbage intake from a sward of S23 perennial ryegrass alone receiving fertilizer N at an annual rate of 360 kg ha⁻¹ were compared with those from a ryegrass-white clover var. Blanca sward given a total N application of 180 kg ha⁻¹. Both treatments gave virtually the same total milk yield of about 12500 kg ha⁻¹ over a 20-week grazing season.     

Dry-matter yield and herbage quality of a perennial ryegrass/white clover sward in a rotational grazing and cutting system

The expected reduction in the use of fertilizer nitrogen (N) on grassland in the Netherlands has led to renewed interest in white clover. Therefore, the performance of a newly sown perennial ryegrass/white clover sward on clay soil was assessed during 4 consecutive years. The experiment consisted of all combinations of two defoliation systems, i.e. one or two silage cuts per year (S1, S2), spring N application rate, i.e. 0 or 50 kg ha⁻¹ year⁻¹ (N₀, N₅₀), and the management system, i.e. rotational grazing and cutting, or cutting only (RGC, CO). The overall mean white clover cover was 30%. All treatments affected white clover cover, which was 8% higher with S2 than with S1, 6% higher with N₀ than with N₅₀ and 12% higher with CO than with RGC. The overall mean annual dry-matter (DM) yield (13·1 t ha⁻¹ year⁻¹) was significantly affected only by the management system: in two relatively wetter years, the annual DM yield was 1·19 t ha⁻¹ higher with RGC than with CO, whereas there was no difference in two relatively drier years. Nitrogen application increased the DM yield in the first cut by 7·0 kg kg⁻¹ N applied, but had no significant effect on the annual DM yield. Herbage quality was not affected by the experimental treatments. The average in vitro organic matter digestibility was 0.801, and the average crude protein content was 193 g kg⁻¹ DM. With the expected reduction in the use of fertilizer N, perennial ryegrass/white clover swards should be seriously considered as an alternative option to perennial ryegrass swards on these clay soils.     

Effects of spring defoliation and fertilizer nitrogen on the growth of white clover in ryegrass/clover swards

An examination was made of the effects of different spring treatments on the growth of white clover in a ryegrass/white clover sward. Plots were either cut once (in February, March or April) or twice (in February and April) or left uncut. Nitrogen was applied to half of the plots in each instance. The clover was sampled at intervals of approximately 3 weeks from February to June to determine numbers of leaves and growing points and weights of plant parts. Rates of leaf appearance were also observed and estimates were made of total herbage mass from ground-level cuts.
Percentages of white clover in the herbage were higher in unfertilized than in fertilized plots and in defoliated than in undefoliated plots. The percentage increases that followed defoliation were usually maintained into later regrowth, showing that clover content was not automatically reduced as herbage mass increased. Increases in growing points were recorded after the beginning of April in defoliated unfertilized plots but not in undefoliated fertilized plots or in plots fertilized and defoliated twice during the spring period, in which numbers fell substantially.
Inverse relationships were found between rates of leaf appearance, or the number of green leaves retained per stolon, and herbage mass, whereas heights of clover and grass leaves and the percentage of dry matter allocated to petiole rather than leaf in the clover increased with increasing herbage mass.
We suggest that the observed differences between spring treatments in clover percentage result primarily from their differential effects on the formation and death of tillers and growing points in the early stages of regrowth.     

Dietary preference of sheep for perennial ryegrass and white clover at contrasting sward surface heights

Non‐pregnant, non‐lactating ewes grazed adjacent monocultures of white clover and perennial ryegrass with three sward surface height (SSH) combinations [6 cm white clover: 6 cm perennial ryegrass (c6g6), 3 cm white clover: 6 cm perennial ryegrass (c3g6), 3 cm white clover: 9 cm perennial ryegrass (c3g9)] at two stocking densities (21·3 or 29·8 ewes ha^–1). Immediately prior to the experiment, all ewes grazed a c6g6 sward. Grazing time on each plant species was recorded during daylight over two 48 h‐test periods. Subsequently, herbage intake rates for each species at each SSH were measured allowing intakes of each species to be calculated. For the first 24 h of both test periods (D1), ewes on treatment c3g6 spent less time grazing white clover than those on treatment c6g6 (228 vs. 362 min) and more time grazing perennial ryegrass (360 vs. 182 min). Total grazing time on treatment c3g6 was more than on treatment c6g6 (587 vs. 544 min) but the difference was not significant. Perennial ryegrass intake was higher (895 vs. 452 g), and white clover intake (814 vs. 1687 g), total intake (1719 vs. 2140 g) and proportion of white clover in the diet (0·460 vs. 0·794) were lower for treatment c3g6 than treatment c6g6. There were no significant differences in total grazing time, grazing time on either species, proportion of grazing time on white clover or proportion of white clover in the diet between treatment c3g6 and treatment c3g9. However, the higher intake rate of perennial ryegrass in treatment c3g9 led to higher perennial ryegrass and total intakes. For the second 24 h of both test periods (D2), ewes on treatment c3g6 again spent more time grazing perennial ryegrass than on treatment c6g6 (270 vs. 161 min) but time spent grazing white clover was similar (318 vs. 308 min). Total grazing time was significantly higher on treatment c3g6 than on treatment c6g6 (588 vs. 469 min) but proportion of grazing time on white clover was similar (0·554 vs. 0·668). Perennial ryegrass intake was significantly higher for treatment c3g6 than for treatment c6g6 (672 vs. 402 g) while white clover intake was significantly lower (1140 vs. 1435 g) but total intake was similar (1812 vs. 1836 g). The proportion of white clover in the diet was significantly lower for treatment c3g6 (0.628 vs. 0.785) than for treatment c6g6. The only significant differences between treatments c3g6 and c3g9 were in perennial ryegrass intake (672 vs. 906 g) and in total intake (1812 vs. 2287 g). Intake of perennial ryegrass on treatment c3g9 was also significantly greater than on treatment c6g6 (906 vs. 402 g) and total intake was higher (2287 vs. 1836 g). At the higher stocking density, time spent grazing perennial ryegrass and perennial ryegrass intake were significantly lower on D1 and D2 while total grazing time was also significantly lower and proportion of time grazing white clover and proportion of white clover in the diet were significantly higher at the higher stocking rate on D2. The results indicate that behaviour changed over the 48 h observation period for treatments c3g6 and c3g9 but behaviour remained relatively constant for animals on treatment c6g6. Ewes traded off dietary preference against total intake by altering grazing times on perennial ryegrass and white clover to achieve maximum net benefit.     

How does the vertical and horizontal structure of a perennial ryegrass and white clover sward influence grazing?

Mixtures of perennial ryegrass ( Lolium perenne L.) and white clover ( Trifolium repens L.) sown in alternate rows or in a thoroughly mixed matrix were grazed by sheep, either continuously or during short grazing tests, and were used to investigate the influence of the vertical and horizontal components of the sward structure on defoliation by sheep.
In an experiment under continuous grazing, the defoliation intensity was greater for white clover compared with perennial ryegrass leaves (0·80 and 0·58 respectively). In spring, perennial ryegrass leaves were more defoliated than white clover leaves, whereas the reverse was observed in summer. The ratio of the proportion of white clover to perennial ryegrass leaves grazed was negatively correlated with the difference between the surface height of the perennial ryegrass and white clover rows in spring. In both spring and summer, white clover leaves of the same extended leaf length had a higher proportion of them grazed than perennial ryegrass leaves.
In another experiment, during short grazing tests with perennial ryegrass–white clover swards that were grazed at the same sward surface height and at the same white clover content as in the previous experiment, there were no significant differences in the proportion of white clover and perennial ryegrass leaves grazed between strips of the two species and thoroughly mixed structures. The proportion of white clover leaves grazed was higher than that of perennial ryegrass leaves.
These results show that the differential defoliation by sheep of perennial ryegrass and white clover leaves varies according to their vertical distribution in the mixed canopy, but is little affected by their horizontal distribution. Even small differences in sward surface height between mixed perennial ryegrass and white clover can affect diet selection by sheep to a rather large extent.     

Growth and colonization of a range of white clover cultivars strip-seeded into an upland perennial ryegrass sward

Seven cultivars of white clover (Trifolium repens L.) (Kent, S184, Huia, Menna, Donna, Alice and Nesta) and a commercial mixture, ‘Ensign’, were strip-seeded into an upland perennial rye-grass (Lolium perenne L.) sward in late June 1986. Swards were first grazed by sheep, either on 5/6 August (early) or on 19/20 August (late) and then every 14–21 days (frequently) or 28–42 days (infrequently) during 1986, followed by a common grazing regime in 1987. During April to mid-June 1988 the swards received either a moderate amount of nitrogen or none and were cut frequently or once only in mid-June. Growth of individual seedlings was assessed before and after grazing during 1986 and stolon accumulation and distribution and sward colonization were assessed during 1987 and 1988. All cultivars emerged rapidly and satisfactorily and there were no consistent significant differences in the overall dry matter accumulation per seedling during establishment. During the first autumn the proportion of the aboveground biomass removed during grazing was smallest in Kent (c. 20%) and largest in Nesta (c. 40%). Kent and S184 produced most leaves and stolons and the greatest length of stolons per seedling and per individual stolon, and Nesta and Alice the fewest leaves and stolons and shortest stolons. Seedlings grazed early had heavier and longer stolons than those grazed late; those grazed frequently had more leaves, stolons and growing points than those grazed infrequently, especially following early grazing. During 1987 Kent and S184 had consistently the largest number of stolon growing points, and weight and length of stolons per unit area; these two cultivars and Nesta also colonized the sward more rapidly than the other cultivars. All cultivars contributed substantially and similarly to herbage production in late September. There were no residual effects of the 1986 treatments after the summer of 1987. During 1988 additions of nitrogen fertilizer at 100 kg N ha^-1 or allowing the herbage to remain undefoliated between mid-April and mid-June both independently halved the number of stolon growing points per unit area; together they reduced it by 80%. Nitrogen also, on average, halved stolon weights but less so in Nesta, Alice and Huia and more so in all other cultivars. Infrequent defoliation greatly decreased stolon weights in Kent and S184 but had no significant effects on the other cultivars. Sward colonization was almost complete by June and entirely so by October for all cultivars in all treatments. Implications of the results for the after-management of strip-seeded white clover are discussed.     

Quantifying the effect of nitrogen fertilizer applications in spring on white clover content in perennial ryegrass-white clover swards

Data are presented for the fourth (1979) and fifth (1980) harvest years of a trial in which four levels of N fertilizer (0, 30, 60 and 90 kg N ha⁻¹) were applied each spring to swards with grass (perennial ryegrass cv. Barlenna) and each one of four cultivars of while clover (Blanca, Sabeda, Olwen and S100) or grass alone. Results from the first three years have been published.
Dry matter (DM) harvested dropped from the fourth to fifth years over all swards by 1 −5 to 2 0 t ha⁻¹ but response to N was maintained (17.4 and 24.4 kg DM (kgN)^−l in response to 90 kg N ha⁻¹ in 1979 and 1980 respectively) despite reductions in summer yields relative to unfertilized swards. Blanca swards produced significantly less DM harvested than all other cultivars in 1979 and all cultivars in 1980 except for Olwen. Clover DM harvested continued to fall from 1977, the mean for the unfertilized treatment in 1980 being 55% of that in 1979. Up to 1980 Sabeda swards produced more clover DM harvested than Blanca swards. Linear regressions between annual clover content at zero N and at each spring N level for each sward type over 5 harvest years were very highly significant. It is concluded that all cultivars used responded similarly to spring N. Further work to develop a method for predicting the effect of spring N on clover content of given swards is required.