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【研究目的】建立可在临床上检测鸡传染性支气管炎抗体的检测IBV抗体的间接ELISA方法;【方法】以表达的重组IBV NP 蛋白为包被抗原,将之包被于聚苯乙烯酶标板上,以封闭液进行封闭,然后加待检血清在37℃作用1h,洗涤后加酶标兔抗鸡IgG抗体,在37℃作用1h,加底物显色,终止反应后测定OD值;【结果】抗原包被浓度为1.45mg/ml,待检血清最佳稀释度为1:40,酶标二抗最佳工作浓度为1:1000,确定阳性判定标准为OD450≥0.314。NP-ELISA与AIV H9、H5、H7、IBD、ND、EDS76 的标准阳性血清不发生交叉反应,具有良好的特异性;【结论】NP-ELISA具有特异性强、灵敏度高、重复性好、方便快速的特点,可用于临床IB抗体的检测。 相似文献
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黄曲霉毒素B1检测ELISA条件的优化 总被引:1,自引:0,他引:1
采用黄曲霉毒素B1与牛血清白蛋白偶联物(AFB1—BSA)抗原免疫Balb/C小鼠获得特异性抗体。以AFB1—STI为检测抗原,利用此抗体,进行间接竞争ELISA法试验。通过正交实验确定了最佳抗原包被反应质量浓度为1.0μg/mL;最佳抗原包被条件为:4℃过夜,一抗和酶标二抗IgG—HRP;最佳稀释度为1∶200000和1∶5000。在此优化条件下制备的抗体检测黄曲霉毒素B1具有很高的特异性和灵敏性,具有实际应用价值。 相似文献
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为了检测猪血清中猪流行性腹泻病毒抗体水平,用纯化的猪流行性腹泻病毒N基因重组表达蛋白作为包被抗原,建立了检测猪流行性腹泻病毒抗体的间接ELISA方法。ELISA的最佳工作条件是:抗原包被浓度为0.5μg/m L,包被时间4℃过夜,5%脱脂乳的PBS封闭37℃2 h及4℃过夜。血清稀释度为1∶100;37℃作用45 min,辣根过氧化物酶标记兔抗猪Ig G稀释度为1∶10 000,37℃温育60 min,底物显色37℃15 min。抗体临界值为OD450nm≥0.30判为阳性,OD450nm≤0.27判为阴性,介于二者之间判为可疑。经重复性试验和交叉试验,结果表明,该方法特异性强、灵敏度高、重复性好。用已建立的ELISA方法检测临床血清样本184份,总阳性率为68.5%。 相似文献
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河南省兔球虫感染情况及种类调查研究 总被引:3,自引:3,他引:0
为了掌握河南省兔球虫感染情况,用麦克马斯特虫卵计数法和饱和氯化钠溶液漂浮法对来自河南省不同地区的11个兔场共465份新鲜粪便进行兔球虫感染情况调查和球虫种类形态鉴定。结果发现:兔球虫平均感染率为74.6%,多数为混合感染,感染球虫种类多在4~6种,以1—3月龄幼兔感染率最高,达83.0%,并且感染强度OPG值最高,平均为6.8×104。本次共检出11种艾美耳球虫,其中大型艾美耳球虫、无残艾美耳球虫、穿孔艾美耳球虫、肠艾美耳球虫为优势种。本实验结果为进一步有效控制兔球虫病和筛选兔球虫疫苗提供重要的参考依据。 相似文献
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无角多赛特绵羊球虫感染情况和种类调查初报 总被引:1,自引:0,他引:1
应用饱和盐水漂浮法和麦克马斯特法对河南某种羊场新引进的82只1岁左右无角多赛特绵羊球虫感染情况和种类进行了调查,结果表明:10圈绵羊均感染球虫,感染率28.57% ̄100%,平均为84.2%(69/82);多为2 ̄5种球虫混和感染;平均OPG值为839.03(200 ̄4200)。共检出11种艾美耳球虫,分别是:韦不理吉艾美耳球虫(Eimeria weybridgensis)、巴库艾美耳球虫(E.bakuensis)、小型艾美耳球虫(E.parva)、类绵羊艾美耳球虫(E.ovinodalis)、颗粒艾美耳球虫(E.granulosa)、槌形艾美耳球虫(E.crandllis)、阿撒他艾美耳球虫(E.ahsata)、浮氏艾美耳球虫(E.faurei)、贡氏艾美耳球虫(E.gon-zalezi)、爱缪拉艾美耳球虫(E.aemula)、苍白艾美耳球虫(E.pallida),其中前4种为优势虫种。用Miaspro图象分析处理系统软件对各种卵囊进行了测量,并进行了显微照相和形态描述。 相似文献
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为了猪圆环病毒2型(PCV2)抗体,本研究建立了快速、敏感的ELISA诊断方法。本研究应用原核表达的PCV2-Cap蛋白作为包被抗原,通过抗原最适包被浓度和兔抗猪IgG最佳稀释浓度的确定,待检血清稀释度的选择,阴阳性临界值的确定,然后进行特异性测定、重复性测定和符合率比较等,建立检测PCV2抗体的间接ELISA方法。结果显示,建立的间接ELISA方法只能检测出PCV2阳性血清;3次重复性试验的差异不显著;与韩国JBT公司的PCV2试剂盒的符合率达到了92.5%;对采集福建省内多个猪场204份血清样品进行检测,其阳性率达到了76.5%。实验表明,建立的检测PCV2抗体的间接ELISA方法具有特异性强、重复性和稳定性好的特点,可用于PCV2抗体的血清学诊断和流行病学调查。 相似文献
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复方中药制剂“球宁”对感染柔嫩艾美耳球虫雏鸡红细胞免疫功能的影响 总被引:1,自引:0,他引:1
用青蒿、常山等组成复方中药制剂“球宁”,并对其进行超微粉碎加工成微粉制剂。按不同比例添加与饲料中,饲喂人工感染柔嫩艾美耳球虫的雏鸡,以磺胺喹啉钠为对照,在感染后8d剖杀,检测血浆中红细胞C3b受体(RBC-CR1)花环率和免疫复合物(RBC-IC)花环率,以观察“球宁”及其微粉制剂对人工感染柔嫩艾美耳球虫的雏鸡的红细胞免疫功能的影响。结果,粗粉组(2%)、微粉组(2%)、微粉组(1%)三组的红细胞C3b受体花环形成率比阳性对照组有明显提高(p<0.05);红细胞免疫复合物(RBC-IC)花环形成率比阳性对照组有所降低(p<0.01)。说明所试药物能改善红细胞的免疫粘附功能,显著降低血浆中免疫复合物的含量,降低球虫病对机体的损伤。 相似文献
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介绍了运用冷冻干燥技术进行速溶蛋花汤的加工,研究了生产工艺、特点以及影响产品质量的主要因素。试验表明,采用冷冻干燥技术加工的速溶蛋花汤,具有营养丰富、风味独特、方便快捷等特点。 相似文献
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Efficiency of water use of early plantings of sunflower 总被引:4,自引:0,他引:4
M. Auxiliadora Soriano Francisco Orgaz Francisco J. Villalobos Elías Fereres 《European Journal of Agronomy》2004,21(4):465-476
Rain fed crop production in Mediterranean environments depends to a large extent on strategies that avoid the intense summer drought. Early plantings of sunflower have given consistently higher yields in such environments, but the basis for such yield increases has not been explored. We conducted two field experiments at Cordoba (Spain) to investigate the effects of an early and a late planting date on the components of water-limited crop productivity; namely, water use (T), water use efficiency (TE) and harvest index (HI) of sunflower. The results were generalized by simulating rain fed sunflower yields, under early (1 January) and late (15 March) plantings, for a 25-year period with the aid of a simulation model of the Ceres type (OILCROP-SUN) which has been validated in Cordoba. Experimental seed yields of early plantings in 1989 and 1996 were 2.0 and 3.0 t ha−1, while late plantings yields were 1.3 and 2.4 t ha−1, for the 2 years. Average simulated yields were 2.7 ± 1.1 and 1.9 ± 0.7 t ha−1 for early and late plantings, respectively. For the 2 years, T of early plantings was higher than that of late plantings, but the response of TE and HI to planting date was not the same in the two experiments. In the simulation exercise, T and TE of early plantings were consistently higher than those of late plantings, while there were no differences in the HI for the two planting dates. We conclude that early plantings of sunflower increase rain fed yields by increasing both T and TE, while the impact of planting date on HI very much depends on the crop water stress pattern, which is quite variable from year to year even in the predictable Mediterranean environment. 相似文献
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The cotyledon and hypocotyl of eight varieties of alfalfa( Medicago sativa L. )are used to study the effect of different cultivation procedures and genotypes on embryogenic callus and embryoid induction,plant development and transplant of alfalfa.The varieties of alfalfa with high embryoid induction rate and high differentiation rate are screened. A high frequency regeneration system for alfalfa tissue culture is established:which is benifical to the gene transformation of alfalfa. 相似文献
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Automobile comfort dynamic simulation is necessary when we predict and evaluate the comfort of a automobile or optimize performance of automobile. The nine DOF vibration model of automobile is established , which acts root mean square value of body in multiple work conditions as comfort evaluation indices. Based on the comfort simulation, the Hongyan 1160 is predicted in the way of comfort. The programmes can be used in many different automobiles . The computational result indicates that the model can simulate vibration of automobile truly. It is significant to research automobile comfort evaluation to optimize performance of automobile and comfort simulation .The computational program can be applied to optimize or simulate which has some reference value. 相似文献
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DENG Zhao-jing 《保鲜与加工》2005,(1):153-156
A new definition of inertia,i.e. the momentum is the measuring of inertia of matter, is proposed. The article through and etc., subjects, the correctness of the new notion of inertia:The monentum is the measuring of inertia has been demonstrated, and the unsolved contradictions of the traditional definition of inetia. And the mass is the measuring of inertia has been revealed. 相似文献
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母猪繁殖障碍成因分析 总被引:3,自引:0,他引:3
繁殖障碍是导致母猪淘汰的最主要因素,直接造成母猪非生产天数延长,繁殖猪群生产效率降低,严重影响猪场的经济效益。本文作者对引起母猪繁殖障碍的多方面因素,包括营养因素(如饲养水平、饲料品质、断奶日龄)、环境因素(如温度、湿度、通风、光照)、疫病因素、生产技术及管理因素进行了详细阐述。 相似文献
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紫玉米不同组配方式的花青素含量及产量比较 总被引:2,自引:0,他引:2
以18个玉米自交系组配成的16个紫玉米杂交组合为试验材料,研究不同组配方式配制的紫玉米籽粒、穗轴、苞叶、雄穗花青素含量及花青素产量。结果表明:(1)不同组配方式的紫玉米籽粒花青素含量、籽粒产量及籽粒花青素产量差异均较大。深紫玉米×深紫玉米,花青素含量最高;深紫非糯玉米×糯玉米,籽粒产量最高;深紫玉米×深紫玉米,籽粒花青素产量最高。(2)深紫玉米×深紫玉米,穗轴花青素含量、花青素产量最高;非紫苞叶×深紫苞叶,紫玉米苞叶花青素含量、花青素产量最高;非紫雄穗×深紫雄穗,紫玉米雄穗花青素含量、花青素产量最高。(3)穗轴花青素含量与籽粒花青素含量呈极显著正相关;雄穗花青素含量与苞叶花青素含量呈极显著正相关。(4)紫玉米花青素总产量(籽粒、穗轴、苞叶、雄穗)为:深紫×深紫>深紫×浅紫>非紫×深紫>浅紫×非紫;籽粒花青素产量对总花青素产量贡献最大。 相似文献