Effects of soil fumigation with Telone C on soil and plant nitrogen,verticillium wilt and yield of Norland and Norgold potatoes

Two fields in Weld County, Colorado were fumigated in the fall or spring with Telone C in an attempt to restore their productivity. The soils were Julesburg sandy loams and had been cropped to either corn or potatoes for many years. Observations were made over three years on the following: soil ammonium and nitrate nitrogen carry over, seasonal changes in these two forms of nitrogen, petiole nitrate concentration,Verticillium population, verticillium wilt expression,Verticillium stem infection and potato yields of two varieties. No significant differences occurred between control and fumigated blocks in the carry over of soil ammonium or nitrate nitrogen. During the growing season of the first year, no significant differences in soil nitrate concentrations occurred, while during the second and third year at certain times, all fumigated blocks contained significantly less nitrate nitrogen than the control block. Significant seasonal differences measured between blocks in soil ammonium concentration were not related to fumigation. Petiole nitrate concentrations while similar during the first year, were significantly higher in most fumigated treatments the second year and generally lower than the controls in the third year. Verticillium populations were not consistently affected by fumigation. Fluctuations not associated with fumigation prevented drawing definite conclusions. Both spring and fall fumigation reduced wilt severity in the two varieties. Effects of spring fumigation carried through two seasons while fall fumigation did not. Plant infection, determined by culturing stem sections, corresponded with the wilt severity ratings. Fall fumigation increased market yields of Norland potatoes but the increase was not statistically significant. Usually, both fall and spring fumigation significantly increased market yield of Norgold potatoes and over three years, spring fumigation was significantly better. Yield increases resulting from fumigation were considered greater than could be explained from alteration in the soil ammonium to nitrate ratios and/or verticillium wilt control as measured in this study.     

Controlling parasitic nematodes and soil-borne diseases of potatoes with soil fumigation

The fumigation of soil infested with a moderate population of parasitic nematodes and fungi very effectively improved the growth, quality and yield of potatoes. The beneficial effects of fumigation with DD and Vorlex were mainly attributed to control of nematodes, particularly the meadow nematode. However, the fumigants did effectively reduce the population ofRhizoctonia solani on potato roots and “scurf” on tubers caused byR. solani. Also less vascular browning of tubers developed in fumigated soil. Thus the effects of soil fumigation were complex, affecting many microorganisms and consequently producing numerous manifestations in the growth of potatoes.     

Pathogenicity of Washington and Oregon isolates of tobacco rattle virus on potato

Soil samples from corky ringspot (CRS) problem fields of potato in the states of Washington and Oregon were collected and planted withNicotiana tabacum ‘Samsun NN’ tobacco to bait tobacco rattle virus (TRV) and to increaseParatrichodorus allius populations, the vector of TRV. Pathogenicity of three isolates of TRV was assessed on Russet Burbank and Russet Norkotah plants usingP. allius as the vector. The most severe CRS tuber symptoms were with TRV from Pasco, WA, followed by Umatilla, OR, and Mattawa, WA, indicating a distinct variation in virulence among virus isolates. The lowest number ofP. allius that transmitted TRV resulting in CRS symptoms on Russet Norkotah was three nematodes per 250 cm³ soil. When potato plants were exposed toP. allius at different times in their growth, tubers on older plants were more resistant than younger tubers to CRS. Severity of CRS tuber symptoms was correlated with age of potato plants in pot culture. Reproduction ofP. allius on potato roots did not influence the incidence and severity of tubers symptoms. Under similar conditions, Bintje, a variety known to be resistant to CRS, served as a host forP. allius, yet remained asymptomatic. The protocol employed in these studies is appropriate for testing potato germplasm for resistance to CRS.     

The relationship betweenVerticillium albo-atrum inoculum density and potato yield

Population studies withVerticillium albo-atrum (microsclerotial form) have shown that a significant negative correlation exists between inoculum density in field soils determined before planting and yield of potatoes in some areas of Colorado but not in others. In Southern Weld and Morgan Counties where potatoes are planted early in sandy soils, reduced yield in Norgold Russet and Norchip cultivars occurred as inoculum densities increased. In Northern Weld County where potatoes are planted later in heavier soils, no such relationship was found. The significant relationship between inoculum density and reduced yield was associated with growing areas where conditions, particularly air temperature, appear to be favorable for verticillium wilt development when plants begin to mature. A minimum number ofVerticillium propagules was required to cause significant yield reductions in total, marketable and U.S. No. 1 grade categories. Below this minimum number, no significant yield reductions occurred. In Southern Weld and Morgan Counties, this minimum number was found to lie between 17.5 and 23 propagules per gram of soil. Controlled greenhouse studies showed that as air temperature decreased from 29.4 C (85 F) to 23.9 C (75 F) more inoculum was required to cause significant disease expression and to shorten plant life. This study has shown that in some areas soil inoculum assays prior to planting may provide a basis for selecting fields which are safe for planting potatoes to avoid severe losses from verticillium wilt. Furthermore, they may help in identifying fields where preventive control measures may be required for satisfactory potato production.     

Elimination of fusarial contamination on seed potatoes

Pieces cut from seed potatoes naturally contaminated with fusarial propagules may produce poor stands if they are planted untreated in cold soils that retard sprouting. In 1972 and 1973, contaminated tubers were given various physical and fungicidal treatments in Maine to eliminate infectious propagules, and planted in North Carolina and Maine. Freshly cut and precut pieces from each of the treatments were evaluated for control of seedpiece decay. Washing tubers improved stands slightly and tended to reduce the number of small plants but did not increase yields. Precut pieces yielded significantly less than fresh cut in Maine and significantly more in North Carolina. All fungicides increased yields at both locations. Thiabendazole and benomyl practically eliminated the inoculum. In a pilot test, pieces cut from tubers sprayed with thiabendazole and benomyl in Maine produced plants and yields in North Carolina that confirmed experimental results and showed that fungicides reduce fusarial inoculum on seed potatoes.     

Chloropicrin Soil Fumigation Reduces <Emphasis Type="Italic">Spongospora subterranea</Emphasis> Soil Inoculum Levels but Does Not Control Powdery Scab Disease on Roots and Tubers of Potato

The effect of chloropicrin fumigation on the soil populations of Spongospora subterranea and the development of powdery scab, formation of root galls and tuber yield was investigated in seven field trials conducted in Minnesota and North Dakota. Sixteen potato cultivars, with different levels of susceptibility to disease on roots and tubers, were planted in plots treated with chloropicrin at rates ranging from zero to 201.8 kg a.i. ha^?1. The amount of S. subterranea DNA in soil was determined using qPCR. Bioassays were conducted to further assess the effect of chloropicrin fumigation on root colonization by S. subterranea in two potato cultivars with contrasting disease susceptibility. In the field, chloropicrin applied at rates between 70.1 to 201.8 kg a.i. ha^?1 significantly decreased S. subterranea initial inoculum in soil but increased the amount of disease observed on roots and tubers of susceptible cultivars. The effect of increasing disease was confirmed in controlled conditions experiments. Although the amount of S. subterranea DNA in roots of bioassay plants increased with increasing chloropicrin rates, it remained similar among potato cultivars. Chloropicrin fumigation significantly increased tuber yield which in cultivars such as Shepody and Umatilla Russet were associated with the amount root galls (r = 0.30; P < 0.03). Results of these studies contradict earlier reports on the use of chloropicrin fumigation for the control of powdery scab. Factors other than inoculum level, such as environmental conditions that affect inoculum efficiency and host susceptibility, may be significant contributors to the development of powdery scab and root gall formation.     

Long term effects of preplant fumigation of potato fields

The application of nematicides resulted in increased potato yields, and populations ofPratylenchus penetrans were lower for 3 years after application; but longevity of benefits of fumigating potato fields varied with the kind and amount of nematicide, application methods and with growing conditions after fumigation. Fumigation of two fields in the fall of 1965 with Vorlex, Telone, D-D, and mixtures of chloropicrin with Telone or D-D resulted in 70% to 90% control of the meadow nematode,P. penetrans, after two crops in one field, and 50% to 70% control after three crops in another. Yield increases of Kennebec potatoes averaged 42% and 16% in successive crops in the first field and yield increases of Katahdin potatoes averaged 13%, 22% and 16% in three successive crops in the other field. Fumigation resulted in 30 to 70% less vascular browning in tubers in the first two crops but there was no effect in the third crop. There was no effect on black scurf in any crop. Root injury varied proportionally with populations ofP. penetrans in roots in 1967. Populations ofP. penetrans regained injurious levels in three other fields after one crop when abundant root growth in moist 1967 followed spring fumigation with Telone and D-D. Under poor conditions for sealing of the soil following fumigation in the spring of 1968, counts ofP. penetrans collected at different soil depths after fumigation showed excellent kill below 3 inches and poorer kill in the upper 2 inches of soil.     

Potato production and incidence ofVerticillium dahliae following rotation to nonhost crops and soil fumigation in the state of Washington

For three years, potato yields, tuber quality, and incidence ofVerticillium dahliae were measured in field plots previously cropped for one year to the following nonhosts: sudan grass, green peas followed by sudan grass (same year), spring wheat, spring wheat followed by sudan grass (same year), sweet corn and field corn. One year rotation to nonhosts did not reduce the population ofV. dahliae propagules in the soil and in only one of the three years wereV. dahliae propagule numbers in potato stems significantly reduced in plots following nonhost crops, compared with plots following potatoes. Verticillium wilt symptoms in potatoes were not reduced by one year rotation to any of these crops and only in one year in three was yield significantly increased. In two of three years, percent U.S. No. 1 tubers was increased following one-year rotation with green peas plus sudan grass, and in one of the two years, specific gravity was increased by rotation. Rotational cropping to nonhosts for two years between potatoes significantly reduced preplant soil populations ofV. dahliae propagules in the soil and in potato stems the following fall. However, soil populations in these two year rotational plots the spring following potato were not reduced compared to plots previously cropped to potatoes two consecutive years. Cropping to nonhosts for two years had no consistent effect on incidence of Verticillium wilt in subsequent potato crops. Two years’ rotation to nonhosts increased plant height and yield compared to continuous cropping to potato but not percent U.S. No. 1 tubers. The various nonhost crops all had about the same non-significant effect on yield. In two out of three years’ trials, fumigation significantly reduced both the incidence of Verticillium wilt and number ofV. dahliae propagules in stems in plots compared to plots non-fumigated. In only one trial, fumigation significantly increased tuber yields and percent U.S. No. 1 tubers.     

Potato germplasm resistant to corky ringspot disease

Potato germplasm was assessed for resistance to corky ringspot disease (CRS) incited by transmission of tobacco rattle virus (TRV). TRV is transmitted by the stubby root nematode,Paratrichodorus allius, in the Pacific Northwest, and characterized by necrotic areas in the tuber. Four tests were conducted at two different problem fields in Umatilla, OR and Pasco, WA. The fields differed in the virulence of the virus isolates. Some clones and named varieties showed resistance only in the field with the relatively mild Umatilla isolate, while others remained symptomless at both sites. The host suitability of test potatoes to threeP. allius populations from the region was determined in greenhouse pot tests, and expressed as reproductive values [R_f = (final population of nematode at 55 days) / (initial population)]. TheR _f values for the nematodes were not correlated with resistance ratings based on visual scoring of CRS symptoms in field grown potatoes. Thus, it appears that CRS resistance is based on reaction of potato genotypes to virus and not the vector. The availability of resistant clones inin vitro form is indicated.     

Fumigation and Fertilizer Nitrogen Source Effects on Potato Yield,Quality, and Early Dying

Research has shown that while fumigation and use of ammonium N can both reduce the severity of verticillium wilt (Verticillium dahliae Kleb.) of potatoes (Solanum tuberosum L.), the use of the two practices together raises concerns over feeding the crop only ammonium N under reduced nitrification conditions. To assess the validity of this concern, we conducted two 3-year field split-plot experiments with both using metam sodium fumigant (none, fall or spring applied) as the main plot. For the first experiment, N source (134 kg N ha^?1 as ammonium sulfate, urea, or ammonium nitrate) was the split, whereas for the second trial in-season N rate (0, 67, 134, or 202 kg N ha^?1 all as ammonium sulfate) was the split. For both trials, in 2 of the 3 years, fumigation significantly increased tuber yield by an average of 9.9 Mg ha^?1 and decreased late-season verticillium severity ratings from 77 to 45%. In some years, fumigation also increased the proportion of U.S. No. 1 tubers and tubers >170 g. No differences in crop yield or quality were observed between the various N sources applied. This was true even on spring-fumigated areas with the highest rate of ammonium N applied. These experiments confirm that the choice between in-season potato N fertilizer should be based on factors such as potential for benefits or N losses, cost, and convenience of use rather than concern over an interaction between fumigation and ammonical N. While both fumigation and N rate reduced verticillium severity ratings in some years, the lack of interaction suggests these factors are functioning independently.     

Evidence for the influence of weeds on corky ringspot persistence in alfalfa and Scotch spearmint rotations

Corky ringspot disease (CRS) of potato is caused by tobacco rattle virus (TRV). The virus is transmitted by the stubby root nematode (Paratrichodorus allius) in the Pacific Northwest potato-producing regions. Alfalfa (Medicago sativa L.) and Scotch spearmint (Mentha cardiaca Baker) rarely serve as hosts for TRV. Therefore,P. allius reared on these plants for 1 to 3 months are cleansed of TRV in greenhouse trials. However, weeds in alfalfa and Scotch spearmint rotation crops may serve as hosts for the virus. In greenhouse trials, hairy nightshade (Solanum sarrachoides), prickly lettuce (Lactuca serriola), henbit (Lamium amplexicaule) and, green foxtail (Setaria viridis) grown alone were found to be suitable hosts ofP. allius, whereas Powell amaranth (Amaranthus powellii) was not. ViruliferousP. allius added to hairy nightshade, prickly lettuce, henbit, green foxtail, or Powell amaranth in mixtures with alfalfa and/or Scotch spearmint occasionally remained viruliferous over a 3-to 4-month period, whereasP. allius maintained on weed-free alfalfa or Scotch spearmint became virus-free after 1 to 2 months. Potato grown in soil containingP. allius that had been maintained on weed-alfalfa or weed-Scotch spearmint mixtures for 3 to 4 months exhibited slight to severe CRS symptoms on new tubers, whereas potato following weed-free Scotch spearmint or alfalfa were free from CRS symptoms. Severe CRS symptoms on potato tubers were only observed when potatoes were grown in soil containingP. allius that were maintained on hairy nightshade or hairy nightshade mixtures with alfalfa or Scotch spearmint. These preliminary data suggest that the presence of weeds that serve as hosts of both TRV and P.allius may nullify the positive effects of growing alfalfa or Scotch spearmint for CRS control. Targeted control efforts of known weed hosts may be required to successfully eliminate CRS from fields using alfalfa and Scotch spearmint rotational crops.     

Efficacy of soil fumigants for control of Verticillium wilt of potatoes

Soil fumigation with materials containing methyl isothiocyanate, 1, 3-dichloropropene, or trichloronitromethane, alone or in combination, delayed “early maturity disease” symptoms caused byVerticillium dahliae Kleb, in potatoes and increased yields. Soil fumigation increased the crop value as much as $818 per acre, depending on the material and rate, used when potatoes were grown for two consecutive years following fumigation. With the exception of trichloronitromethane, fall fumigation was as effective or more effective than spring fumigation.     

Sioux: A scab resistant red potato variety

Sioux is a uniform round, red, slightly scaly russet variety of medium maturity. The variety is resistant to scab (Streptomyces scabies) and susceptible to Fusarium, Verticillium and late blight. In Nebraska, Sioux produces moderate to high yields, but higher yields of U.S. #1 potatoes when compared with the standard red table varieties Norland and Bounty. The specific gravity of Sioux is higher than that of the standard red table and white chipping varieties Haig, Platte and Kennebec. The variety is suitable for chipping and the production of frozen products. When grown as a late summer crop, Sioux is of good color and carries to market in better condition than the standard red varieties. In the 12 States in the North Central Regional Trials, Sioux was equal to the standard variety Red Pontiac in total yield and superior in yield of U.S. #1 potatoes. The specific gravity of Sioux was higher than that of Irish Cobbler or any of the other standard varieties. The yield of Sioux was as high and the specific gravity was higher than any of the standard varieties in Texas and Alabama trials.     

Utilization of potatoes for life support systems in space. I. Cultivar-photoperiod interactions

The productive potential of potatoes (Solanum tuberosum L. cvs. Norland, Superior, Norchip, and Kennebec) was assessed for life support systems being proposed for space stations and/or lunar colonies. Plants were grown in walk-in growth rooms for 15 weeks at 20 C under 12-, 16- and 20-h photoperiods of 400 μmol m^t-2s^t-1 photosynthetic photon flux (PPF). Norland yielded the greatest tuber fresh weight, producing 2.3, 2.4, and 2.9 kg/plant under 12-, 16-, and 20-h photoperiods, respectively. The respective yields for the other cultivars under 12-, 16-, and 20-h were: Superior, 1.9, 1.5, and 1.8 kg/plant; Norchip, 1.8, 1.4, and 2.0 kg/plant; and Kennebec, 2.3, 0.2, and 0.8 kg/plant. Shoot and total plant biomass increased with lengthening photoperiods except for Kennebec, which showed increased shoot growth but no change in total growth with the longer photoperiods. Kennebec shoot growth under the 20-h photoperiod, and to some extent under 16-h, was noticeably stunted with shortened internodes. In addition, leaves of these plants showed mild chlorosis with rusty “flecking” of the surfaces. The harvest index (ratio of tuber yield/total biomass) was highest for all cultivars under the 12-h photoperiod, with a maximum of 0.69 for Norland. Similarly, the tuber yield per input of irradiant energy also was highest under 12-h for all cultivars. The tuber yield expressed on an area basis for the highest yielding treatment (Norland under 20-h) equaled 2.2 kg dry matter m^t-2. Over 15 weeks this equates to a productivity of 20.7 g tuber dry matter m^t-2 day^t-1. Assuming 3.73 kcal per g tuber dry matter and a daily human dietary requirement of 2800 kcal, then 36 m² of potatoes could supply the daily energy requirement for one human. Potential for increasing productivity is discussed.     

Influence of temperature and length of storage on resistance of potato to tuber rot induced byErwinia chrysanthemi

Tubers of 11 clones ofSolanum tuberosum subsp.andigena (andigena) and 12 cultivars ofS. tuberosum subsp.tuberosum (tuberosum) were inoculated with water suspensions (5.5 × 10⁴ 5.5 × 10⁵, 5.5 × 10⁶ CFU/ml) ofErwinia chrysanthemi. Tubers were inoculated immediately after harvest and after 6 and 16 wk of storage at 4 and 23°C. Tuber rot incidence in andigena and tuberosum increased as inoculum concentration increased. Based on tuber rot severity, clones of andigena were classified as resistant, intermediate, or susceptible and cultivars of tuberosum were classified as intermediate or susceptible. Rot severity increased in all tubers stored at 4°C and in tubers of tuberosum stored at 23°C; rot did not increase in tubers of andigena stored at 23°C. Electrolyte leakage (EL), total sugars (TS), reducing sugars (RS), non-reducing sugars (NRS) and dry matter (DM) were determined in non-inoculated tubers. There was a significant positive correlation between the rate of EL, concentration of RS and tuber rot in andigena and tuberosum. Tuber rot and DM were negatively correlated in tubers of andigena; but they were not correlated in tuberosum. Clones of andigena with low EL, TS, RS, and high DM were resistant to tuber rot, and the incidence of tuber rot in these clones was much less influenced by temperature and length of storage. The influence of temperature and length of storage on susceptibility toE. chrysanthemi may be explained by increased cell membrane permeability; increased leakage of accumulated sugars in potatoes stored at 4°C could favor bacterial proliferation resulting in more disease.     

Weed hosts ofParatrichodorus allius and tobacco rattle virus in the Pacific Northwest

The ability of several weed species to serve as hosts for tobacco rattle virus (TKV), the causal agent of corky ringspot disease of potato (CRS), and its nematode vector,Paratrichodorus allius, was investigated in greenhouse studies. ViruliferousP. allius multiplied on 24 out of 37 weed species tested, indicating they were suitable hosts of the vector. However, only 11 of these weeds were infected with TRV, as determined by ELISA. The nonhost status of a given weed species was not changed whether the viruliferous vector population originated from CRS problem fields in WA, OR, or ID. Several weeds served as hosts for the vector and virus including kochia, prickly lettuce, henbit, nightshade species (black, hairy, and cutleaf), common chickweed, and annual sowthistle. Virus-freeP. allius acquired TRV from the three nightshade species, volunteer potato grown from TRV-infected tubers, and prickly lettuce, and subsequently transmitted the virus to ‘Samsun NN’ tobacco indicator plants. Thus, some weeds may play a role in the epidemiology of CRS by perpetuating TRV and its vector in a problem field.     

Response of Russet Burbank potatoes to soil fumigation and nitrogen fertilizers

Two field studies were conducted to determine the effects of soil fumigation on the response of Russet Burbank potatoes (Solanum tuberosum L.) to NH₄-N or NH₄NO₃ fertilizers. In one study fumigation increased yields but N source had no effect. In the other study fumigation had no effect but NH₄-N provided higher yields than NH₄NO₃. Microbial studies indicated that although populations of nitrifying bacteria were initially altered by fumigation, nitrification rates were similar to those in non-fumigated soil. In a controlled environment study in which potatoes were grown in fumigated field soil, neither N source nor fumigation had any effect on growth or yield. These studies indicated that fumigation had no effect on the response of potatoes to NH₄-N or NH₄NO₃ fertilizers.     

Association of viral,bacterial and fungal pathogens with vascular discoloration of Russet Burbank potato tubers

Russet Burbank potatoes from the 1979 and 1980 crop years, collected from Chicago, IL repack warehouses and retail markets, were sampled for vascular discoloration. The amount of discoloration varied among sampling months and states of origin and decreased from 7.7% in 1979 to 1.6% in 1980. Highest levels of vascular discoloration were detected in December and January samples. Vascular discolored and non-discolored (control) tubers were assayed for the presence of potato leafroll, potato virus X, and beet western yellows viruses by the enzyme-linked immunosorbent assay (ELISA). Tubers were also assayed for 2 subspecies ofErwinia carotovora and forVerticillium albo-atrum andV. dahliae. Potato leafroll virus was detected in 31 of 831 vascular discolored tubers.V. albo-atrum was detected in 1 of 180 discolored tubers. Beet western yellows virus was not detected in discolored or non-discolored tubers. Two subspecies ofE. carotovora and potato virus X were equally common in discolored and non-discolored tubers.