共查询到20条相似文献,搜索用时 78 毫秒
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新城疫油佐剂灭能苗免疫调节剂对鸡免疫效果的影响曾明华沈咏舟魏建忠谢三星(安徽农业大学畜牧水产学院,合肥230036)为了提高机体免疫力,克服雏鸡母源抗体在新城疫(ND)免疫中产生的不良反应[1],寻找有效的免疫调节剂不失为一种重要途径。据报道[2]N... 相似文献
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接种不同剂量的新城疫疫苗对鸡免疫应答的影响 总被引:5,自引:1,他引:4
目前在我国使用的新城疫疫苗主要有Ⅰ系、Ⅱ系、F系、LaSota系(Ⅳ系)弱毒疫苗和灭活疫苗。其中Ⅳ系苗因其使用方便,免疫效果较好,引起的反应小,所以在临床上应用较多,并常以饮水免疫,但使用剂量较大。机体的免疫应答能力受抗原用量的影响,在一定的范围内随抗原用量的加大免疫效果也相应增强,但并不是抗原用量越多越好,过高的免疫剂量不但不能提高机体的免疫效果,反而会产生免疫抑制,同时也增加了经济投入。为了给临床应用剂量提供了一个参考标准,我们设计了本试验,现报告如下。 相似文献
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分子筛凝胶浓缩新城疫病毒疫苗的研究 总被引:1,自引:0,他引:1
本文采用新型分了筛凝胶作为浓缩剂对新城疫毒感染鸡胚的尿囊进行了浓缩实验,结果表明,该浓缩胶可使新城疫病毒浓缩96.24倍,病毒的损失量为8.65%,用浓缩胶浓缩新城疫毒后制成灭活苗,浓缩苗进行鸡的免疫实验,以研究浓缩疫苗的免疫效果,浓缩新城疫抗原后灭活苗免疫后8天抗体水平迅速上升,HI为5.6,并持续上升,均高于对照组,说明浓缩疫苗可以使鸡产生较高的抗体水平。 相似文献
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鸡新城疫(ND)是当前养鸡业中危害最大的一种病毒性传染病.lop年底笔者在金竹山乡资江村、中连乡民主村各调查了农户100户,因鸡疫病流行,死亡率分别为82.l%和刀.3%,其中大部分死于ND.而ND免疫接种密度分别为78.l%和79.0%.为什么农村多数鸡群在雏鸡时滴过11系苗或haa苗,有的在中雏还注射过1系苗仍会发生ND呢?本文就影响ND免疫效果的因素予以浅述.l母源抗体的干扰产蛋母鸡若接种过ND疫苗,体内就会产生不同程度的抗ND抗体,这些抗体可以通过蛋的孵化由蛋黄传递给雏鸡,雏鸡就会有抵抗该病毒侵袭的保护力,即具有一定… 相似文献
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Enhancement of mucosal immune responses by intranasal co-delivery of Newcastle disease vaccine plus CpG oligonucleotide in SPF chickens in vivo 总被引:2,自引:0,他引:2
Immunostimulatory CpG oligodeoxynucleotides (ODN) have been tested as immunoadjuvants for various vaccines in mice and human. Findings from previous reports suggest that CpG ODN can be used to enhance magnitude and balance of an immune response while reducing undesirable side effects of commercial vaccine, when delivered by parenteral route. Recently, it has been showed that CpG ODN is a promising mucosal adjuvant in mice, but data on mucosal immune responses induced by CpG ODN in other animals, especially in chickens, are scarce. Herein, we evaluated intranasal (IN) delivery of CpG ODN with newcastle disease (ND) vaccine (NDV) to determine its potential as a mucosal adjuvant to a commercial vaccine. CpG ODN augmented systemic (IgG in serum, T cell proliferation) and mucosal (IgA in intestinal washings and feces) immune responses against antigen. CpG ODN stimulated effectively both systemic and mucosal immune responses when delivered intranasally. Results from this study indicate that stimulatory CpG ODN is a potential effective mucosal adjuvant for the NDV in SPF chickens and may be applicable to husbandry animals. 相似文献
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将40只10日龄海兰褐蛋雏鸡,随机分成试验组和对照组,每组20只,分别皮下注射ND-La Sota弱毒疫苗0.2ml/只。同时给试验组雏鸡投服缓释复方免疫增强剂1粒/只,对照组不投服。在免疫前、免疫后10、20、30和40d,每组随机取10只鸡采血,测定ND-HI抗体效价、T淋巴细胞ERFC形成率和ANAE阳性率。结果免疫后10、20、30、40d,试验组与对照组比较,鸡ND-HI抗体效价、T淋巴细胞ERFC形成率和ANAE阳性率差异极显著(P〈0.01)或显著(P〈0.05),三项指标具有相同的消涨趋势,表明缓释复方免疫增强剂可以提高鸡的体液免疫功能和细胞免疫功能。 相似文献
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用鸡新城疫-传染性法氏囊病二联灭活疫苗(La Sota+HQ株)分别免疫1、7、14日龄SPF雏鸡,1日龄免疫组于25日龄采血,法氏囊病中和抗体几何平均值为15.5log2,攻毒保护10/10;7日龄免疫组于30日龄采血,法氏囊病中和抗体几何平均值为14.8log2,攻毒保护10/10;14日龄免疫组于35日龄采血,法氏囊病中和抗体几何平均值为15.4log2,攻毒保护10/10。3组在50日龄时法氏囊病中和抗体几何平均值依次为16log2、16.1log2、15.9log2,攻毒保护均为10/10。用鸡新城疫-传染性法氏囊病二联灭活疫苗(La Sota+HQ株)免疫带有母源抗体的1、7、14日龄商品肉鸡,免后25 d血清法氏囊病中和抗体几何平均值分别为12.4log2、15log2、14.2log2,攻毒保护分别为8/10、10/10、9/10;3组在50日龄时法氏囊病中和抗体几何平均值依次为16.2log2、15.7log2、15.5log2,攻毒保护均为10/10。 相似文献
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新城疫(ND)—减蛋综合征(EDS76)蜂胶二联灭活疫苗的研制Ⅰ.制苗工艺、疫苗安全性和免疫原性试验 总被引:6,自引:0,他引:6
将新城疫病毒(NDV)Colon-30株和减蛋综合征病毒(EDS76AV)-127株分别接种于非免疫鸡胚和鸭胚,制备抗原液。抗原液经甲醛灭活后,与纯化的天然免疫增强剂蜂胶乳化,制成鸡新城疫(ND)-减蛋综合征(EDS76)蜂胶二联灭活疫苗,对疫苗的安全性和免疫原性进行了测定。对3-6周龄易感雏鸡以2倍使用剂量肌内注射,安全性良好;对1日龄雏鸡接种1个使用剂量进行急性毒性试验。15d生长曲线表明,对雏难 影响。对6周龄雏鸡注射1个使用剂量,每批疫苗免疫10只鸡,免疫后5-32d,鸡血清中EDS76血凝抑制(HI)抗体的几何平均滴度(GMT)为6.5-11.8log2,ND血凝抑制(HI)抗体的几何平均滴度(GMT)为4.8-9.8log2;对ND免疫攻毒试验表明,3批疫苗,每羽份含有的半数保护量(PD50)分别为≥127、≥131和≥127。 相似文献
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Background
As a member of the Paramyxoviridae group, Newcastle disease virus (NDV) is the key causative agent of Newcastle disease (ND) that attacks chickens, turkeys and other avian birds. Surviving birds showed lower feed utilization, growth performance or egg production, which results in severe economic losses. The purpose of this study was to determine the effect of different doses of NDV immunization on growth performance, plasma variables and immune response of broiler chickens.Methods
A total of 480 one-day-old Arbor Acres broilers were randomly administrated with 0, 4, 6 or 8 doses of NDV at 12 d and 28 d, respectively. Each group consisted of ten replicates with 12 birds each. Growth performance and organ weight were recorded. Plasma concentration of glucose, total protein, cholesterol, triglycerides and nonesterified fatty acid was determined using commercial kits. The concentration of plasma corticosterone and insulin was measured using commercially available radio immune assay kits. Serum antibody titer and peripheral blood lymphocyte proliferation were also recorded.Results
The results showed that NDV decreased body weight gain (BWG), and increased Feed:Gain ratio at 1–21 d at all doses (P < 0.05). Plasma insulin concentration was lower in all immunization groups after the first immunization at 12 d (P < 0.01). The rest of the plasma indexes were not affected by NDV immunization, including glucose, total protein, cholesterol, triglycerides, nonesterified fatty acid, heterophil/lymphocyte ratio, as well as the proliferation of peripheral blood lymphocyte (P > 0.05). Compared with the control group, NDV treatment elevated NDV antibody titer at 10 d after the first inoculation (P < 0.05), and at d 5, 9 and 13 after the second inoculation (P < 0.05). Repeated NDV inoculation had no deleterious impacts on body composition at 42 d, and nutrient accretion rates at 8–42 d (P > 0.05).Conclusions
In conclusion, NDV challenge decreased BWG and feed efficiency in earlier stage of growth. However, NDV treatment at 6 doses down-regulated the Feed:Gain ratio by 6.36 % throughout the whole growing period. These data suggest that appropriate lower doses of NDV inoculation increase feed efficiency of broiler chickens.Electronic supplementary material
The online version of this article (doi:10.1186/s40104-015-0019-y) contains supplementary material, which is available to authorized users. 相似文献19.
鸡新城疫(ND)是一种急性、高度接触性传染病,是危害我国养鸡业最严重的烈性传染病之一。用H120、C30疫苗、C30 油剂苗对广西大法鸡进行新城疫免疫,经过一定免疫期,采血,分离血清样品,用β-微量血凝抑制试验检测其抗体效价。其结果显示:用C30 油剂苗,在同一批次、同一段时间内新城疫抗体水平最高,其免疫效果最好。 相似文献
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将未浓缩的新城疫抗原分别与未浓缩的、浓缩3倍、浓缩6倍的禽流感抗原混合,并制备成三组鸡新城疫、禽流感(H9N2 HP株)二联灭活疫苗(简称新-流二联灭活疫苗),分别免疫21日龄SPF鸡,每羽0.3 mL,同时设置未免疫的空白对照组,免疫组与对照组均在免疫前及免疫后7、14、21、28、35 d进行采血,检测新城疫和禽流感抗体。结果发现,各免疫组在免后不同日龄的新城疫抗体基本一致,禽流感病毒抗原浓缩倍数越高(即禽流感病毒含量越高)的新-流二联灭活疫苗,免后14、21 d的抗体也越高;从免后21 d开始,各免疫组的禽流感抗体水平差异逐渐减小,免疫后禽流感抗体水平的高低可以反映该疫苗的免疫效果。试验结果表明,该疫苗可以通过浓缩提高抗原病毒含量的方法来提高免后早期抗体水平,取得良好的早期免疫效果。 相似文献