Propagation of Porcine Cytomegalic Inclusion Disease Virus In Cell Cultures — Preliminary Report

The successful propagation of porcine cytomegalic inclusion disease virus (inclusion-body rhinitis virus) in primary pig lung cell cultures is reported. CID virus was carried through five passages in cell cultures with cytopathic affects appearing from 11 to 18 days post-inoculation. Four pigs inoculated with infected cell culture fluids from the second cell culture passage remained clinically normal. Two, however, had typical inclusion bodies in the glands of the nasal mucosa when examined three weeks post-inoculation. The progressive cytopathic effect produced and the inclusion bodies formed by this strain of porcine CIDV are described. These inclusion bodies appeared to be similar to those formed by cell culture-propagated cytomegaloviruses of man, mouse and guinea pig.     

Newcastle Disease Strain F. Virus — A Review

Strain F Newcastle disease virus is a virus of low virulence originally reported by Asplin (1952) in England. Since that date, the use of this virus as an immunizing agent in the form of a live vaccine, has been studied. As a result, Strain F Newcastle disease vaccine has been used in national and experimental control programs in several countries in Europe, Africa and Asia. The published literature is reviewed under the following headings: properties, viability, clinical effects of vaccination, duration of immunity and a simultaneous Newcastle disease fowl pox vaccination. This review includes 24 reports published outside North America.     

鸡传染性法氏囊病病毒适应于Vero细胞的初步研究

将国内６个省市（广东、巾东、河北、辽宁、、新疆和北京）的７株鸡传染性法氏囊病（ＩＢＤ）的法氏囊组织处理后，直接在Ｖｅｒｏ细胞上盲传３～４代，均能不同程度的产生特征性细胞病变效应（ＣＰＥ），并通过选用具有广谱性的抗法氏囊病病毒的单克隆抗体（ＩＢＩ）Ｖ－ＭｃＡｂ）采用间接免疫荧光（ＩＦＡ）和碱性磷酸酶—抗碱性磷酸酶桥联酶显色技术（ＡＰＡＡＰ）的方法对７株分离毒的Ｖｅｒｏ第８代细胞进行鉴定，又用逆转—聚合酶链式反应（ＲＴ－ＰＣＲ）对７株分离毒的第１４代Ｖｅｒｏ细胞毒进行进一步证实，结果表明这７株ＩＢＤ囊毒均适应于Ｖｅｒｏ细胞上，这是国内首次报道将组织毒直接适应于Ｖｅｒｏ传代细胞上。     

鹅的几种主要病毒性传染病流行特点及防制对策

本文就鹅的病毒性传染病——小鹅瘟、鹅副粘病毒病、鹅禽流感的症状、病变、诊断、防制加以介绍,以便控制这儿种病毒性传染病的流行和蔓延。