土霉素在黑鲷体内的药物代谢动力学研究

首次报道了黑鲷口服土霉素的药物代谢动力学特征，用高效液相色谱法测定组织中的药物含量，药物在肌肉，血液，肝脏中的平均回归率分别为８５.61%,85.38%,82.005,该方法的检测限可达0.01μg/g，黑鲷１次口服剂量为７５mg/kg的土壤素后，其血液药物浓度－时间数据符合一室开放动力学模型，吸收速率常数（ka)为０.296/h，达峰时间（Ｔamx)为１０.635h,峰浓度（Ｃmax)为１.398μg/ml, 分布半衰期（Ｔ１／２a）为２.339h ,消除半衰期(T1/20β）为46.663h，药时曲线下面积（ＡＵＣ）为１１0.25mg/L.h，黑绸口服药物０.5h后在血液，肥肉，肝脏，肾脏４种组织中就可以检测到药物的存在，药物在１６h的采样点浓度达最高，分别为1.68μg/ml,1.68,2.52,6.77μg/g。     

两种给药途径土霉素残留在鲫鱼体内的消除规律

探讨了在肌肉注射和口灌给药方式下土霉素残留在鲫鱼组织内的消除情况。结果表明: 鲫鱼肌肉注射和口灌给药后土霉素在体内的最大浓度依次是: 肌肉 4 636mg/kg和 2 869mg/kg, 血液 5 467mg/kg和 3 112mg/kg, 肝脏 7 165mg/kg和 5 086mg/kg, 肾脏 8 308mg/kg和 5 017mg/kg; 肌肉注射第 288h时除肾脏中的土霉素浓度仍然高达 0 375mg/kg, 其他组织中已经检测不到土霉素的存在, 而口灌给药第 288h时肌肉、血液、肝脏、肾脏中土霉素的浓度依次为: 0 133mg/kg, 0 237mg/kg, 0 272mg/kg, 0 272mg/kg。因此, 在 (10±1)℃水温条件下, 以剂量 50mg/kg鱼体重单次给药, 肌肉注射各组织内的药物浓度比口灌给药高, 口灌给药消除速度比肌肉注射慢。本次实验结果建议在 (10±1)℃以上水温条件下, 无论肌肉注射还是口灌给药, 建议第 12天以后捕鱼食用是安全的。     

土霉素在鱼体内的动力学研究概况

综述土霉素在鱼体内的动力学研究概况。分析动力学中各个相关过程(包括吸收、分布、代谢、排泄)的特点，以及影响土霉素在鱼体内过程的各种因素。     

盐酸土霉素在奥尼罗非鱼体内的药代动力学及残留研究

在水温(25±2)℃条件下,以15 mg/kg鱼体重的剂量给奥尼罗非鱼单次口灌盐酸土霉素,采用高效液相色谱法测定血浆和肌肉组织中的药物浓度,研究盐酸土霉素在奥尼罗非鱼体内的代谢及消除规律。结果显示:血药时间数据符合一级吸收二室开放模型,半衰期(T1/2Ka、T1/2α、T1/2β)分别为4.79、4.10、45.20 h,最大血药浓度为1.50μg/m L,达峰时间为7.30 h,药时曲线下面积(AUC)为42.35μg·h/m L。肌肉作为可食性组织,选取肌肉组织作为残留检测的靶组织,以0.1 mg/kg为最高残留限量,在本试验条件下,建议休药期不低于10 d。     

恩诺沙星及其代谢产物环丙沙星在拟穴青蟹体内的药代动力学

采用高效液相色谱法,研究盐度33条件下恩诺沙星口灌和肌肉注射给药(剂量10 mg/kg)后,恩诺沙星及其代谢物环丙沙星在拟穴青蟹体内的药代动力学和组织分布。血淋巴和组织中药代动力学参数采用基于统计矩原理的非房室模型进行计算。恩诺沙星口灌和肌肉注射拟穴青蟹给药后,血药达峰快,分别为0.5 h和1 min,达峰浓度分别为12.90和31.86 μg/mL,曲线下面积(AUC)分别为216.1和816.8 μg/(mL·h)。恩诺沙星在拟穴青蟹组织中分布较广,口灌给药下肌肉和肝胰腺AUC分别为445.9和817.6 μg/(g·h),肌肉注射给药下的AUC分别为554.7和2 573.7 μg/(g·h)。与其它水产动物相比,恩诺沙星在拟穴青蟹体内消除速度为中等水平,口灌和肌肉注射恩诺沙星后血药消除半衰期(t_1/2z)分别为26.45和57.02 h,总体清除率(CL_z)分别为0.054和0.012 L/(h·kg)。恩诺沙星在拟穴青蟹体内代谢生成环丙沙星的量较少,口灌给药下血淋巴、肌肉和肝胰腺的AUC_CIP/AUC_ENR分别为6.66%、3.66%和4.78%,肌肉注射给药下,其相应值分别为4.16%、7.24%和1.48%,在拟穴青蟹体内起药效作用仍是以恩诺沙星为主。以C_max/MIC、AUC_0-24/MIC评价恩诺沙星在青蟹体内的药效作用,建议给拟穴青蟹以10 mg/kg剂量每隔24小时投喂一次恩诺沙星,对弧菌引起的细菌性疾病具有较好的防治效果。     

甲砜霉素在鲫体内的药物代谢动力学研究

采用液相色谱串联质谱法,研究通过单剂量口灌给药,对甲砜霉素在鲫(Carassius auratus)体内的药代动力学进行研究,为甲砜霉素在鲫疾病预防和治疗方面提供理论基础。给药剂量为30 mg/kg体重,实验水温(20±0.2)℃,鲫平均体重(40.70±7.87)g。取给药后0.25、0.5、1、1.5、2、4、6、8、12、24、36、48、72 h鲫的肌肉、血浆、肝脏、肾脏,测定各组织中甲砜霉素的浓度,用药动学3p97软件进行数据的处理和分析。结果表明:甲砜霉素在鲫体内吸收分布迅速,符合一级吸收二室开放模型,但消除缓慢。甲砜霉素在鲫血浆、肝脏、肾脏和肌肉中的主要药代动力学参数如下:分布半衰期(T1/2α)分别为1.446 h、1.958 h、7.410 h和1.376 h;消除半衰期(T1/2β)分别为16.712 h、21.267 h、79.970 h和25.600 h;药时曲线下总面积(AUC)分别为669.073μg/(mL.h)、271.260μg/(g.h)、3616.060μg/(g.h)和158.634μg/(g.h)。甲砜霉素在水产动物体内吸收快,肌肉和肾脏消除半衰期长,消除缓慢,因此,在该类药物使用时,应相对延长给药间隔时间,避免耐药性的产生。     

伊维菌素在鲫体内的药代动力学

以0.4 mg/kg的给药剂量进行口灌和肌肉注射给药,研究伊维菌素(IVM)在鲫体内的药代动力学。两种给药方式下,鲫组织中的IVM药—时曲线大都呈现多峰现象。肌肉注射给药后,药动学统计矩参数为Cmax=0.445 mg/L、Tmax=48 h、t1/2z=524.2 h、MRT(0-∞)=788 h、AUC(0-∞)=289.2(mg/L).h;口灌给药后,药动学统计矩参数为Cmax=0.264 mg/L、Tmax=8 h、t1/2z=153.9h、MRT(0-∞)=269.78 h、AUC(0-∞)=83.77(mg/L).h。两种给药方式相比,口灌组鲫对药物的吸收和清除均较快,而肌肉注射组鲫各组织中的药物浓度高,AUC值也较大。两种给药方式下,IVM在鲫各组织中AUC(0-600)值呈现相同的排列顺序,由大到小分别为性腺、血液、肾脏、肝胰脏、肌肉。IVM在鲫性腺和肾脏中均具有一定的蓄积作用,其主要表现为药物浓度高,MRT值大,且清除率低于血药的清除率,其中卵巢的积蓄作用最为明显。25℃的水温条件下,肌肉注射给药后,鲫休药期应不低于25 d;口灌给药后,鲫的休药期应不低于15 d。休药期与水温条件和给药剂量有关,因此在养殖生产过程中的休药期要根据实际情况适当调整。     

2种水温条件下罗非鱼体内氟苯尼考的药物动力学比较

采用药饵给药,药物剂量为10mg·kg^-1,比较研究了22和28℃水温条件下奥尼罗非鱼（Oreochromis niloticus×O．aureus）体内氟苯尼考的药物动力学。结果得出,22℃组和28℃组罗非鱼血浆的峰药浓度（Cmax）分别为4．46和3．90μg·mL^-1,达峰时间（Tmax）均为12h,消除半衰期（T1/2β）分别为10．03和8．12h,药-时曲线下面积（AUC）分别为86．68和72．44h·μg·mL^-1。相应条件下的肌肉Cmax分别为6．88和4．59μg·g^-1,Tmax均为12h,T1／2β分别为10．97和8．03h,AUC分别为112．71和73．66h·μg·g^-1。低温组罗非鱼血浆和肌肉中药物的T1／2β均长于高温组,前者分别比后者长1．91和2．96h,表明低温组罗非鱼体内药物的消除速度慢于高温组。虽然2个水温组血浆和肌肉中药物的Tmax相同,但低温组血浆、肌肉的Cmax和AUC均明显高于高温组,表明低温组罗非鱼吸收利用药物程度高于高温组。     

氟苯尼考在鲫和草鱼体内的药代/药效动力学联合参数及其临床给药方案的研究

为研究氟苯尼考在鲫和草鱼体内的药代学、药效动力学联合参数,并制定氟苯尼考对鲫、草鱼的精确用药方案,本实验结合氟苯尼考对致病性嗜水气单胞菌CAAh01的体外药效学研究和口灌不同剂量的氟苯尼考在鲫、草鱼体内药代动力学研究,确定了氟苯尼考防治该致病菌引起的鲫和草鱼细菌性败血症的给药方案。研究结果显示,氟苯尼考对CAAh01菌株的最小抑菌浓度(MIC)为0.5μg/mL,最小杀菌浓度(MBC)为1.0μg/mL,防细菌耐药突变浓度(MPC)为6.0μg/mL,防耐药突变选择窗(MSW)为0.5~6.0μg/mL。按10、20、30 mg/kg体质量剂量对鲫、草鱼口灌氟苯尼考后,在鲫体内,血药浓度大于MPC的维持时间分别为5、8、24 h;AUC24/MIC分别为177.06、265.90、426.50;Cmax/MIC分别为15.59、21.32、31.24。在草鱼体内,血药浓度大于MPC的维持时间分别为0、0、3 h;AUC24/MIC分别为38.60、75.08、121.94;Cmax/MIC分别为4.75、10.08、19.99。研究表明,综合血药浓度维持MPC以上的时间、AUC24/MIC或Cmax/MIC指标,氟苯尼考适用于鲫细菌性疾病的防治,其防突变用药方案为剂量30 mg/kg,每日1次给药,休药期不低于20 d。对于草鱼细菌性疾病的防治,氟苯尼考不宜连续使用。     

土霉素在奥尼罗非鱼体内的药动学研究

在(21±1)℃的水温条件下,以50 mg/kg的单剂量,分别给奥尼罗非鱼(Oreochromis aureus×O.niloticus)水剂口灌和混饲口灌土霉素,用高效液相色谱法(HPLC)检测给药后各个时间点的血药浓度。结果显示:最低检测限为0.005μg/mL,线性范围为0.005~4μg/mL。水剂口灌组和混饲口灌组的药时数据均符合具时滞的二室开放动力学模型,水剂口灌组的动力学方程为:Ct=0.231e-0.028(t-0.010)+0.353e-0.011(t-0.010)-0.584e-0.468(t-0.010),混饲口灌组动力学方程:Ct=0.839e-0.057(t-0.459)+0.442e-0.013(t-0.459)-1.281e-0.282(t-0.459)。水剂口灌组及混饲口灌组主要药动学参数分别为:吸收半衰期(t1/2ka)为1.481 h,2.458 h;分布半衰期(t1/2α)为24.834 h,12.193 h;消除半衰期(t1/2β)为60.312 h,51.533 h;达峰时间(Tmax)为7.230 h,8.221 h;最大血药浓度(Cmax)为0.494μg/mL,0.796μg/mL;血药浓度-时间曲线下面积(AUC)=37.74μg.h/mL,43.075μg.h/mL。这些参数表明,水剂口灌比混饲口灌吸收快,分布和消除慢,在血液中达到峰浓度的时间更短,但峰浓度值比混饲口灌低。     

Pharmacokinetics and tissue distribution of oxytetracycline in carp, Cyprinus carpio L., following different routes of administration

Abstract. The objective of this pharmacokinetic study was to investigate absorption, distribution, elimination and bioavailability of oxytetracycline (OTC) in carp, Cyprinus carpio L ., after different routes of administration, OTC was administered intravenously (i.v.), intramuscularly (i.m.) and orally at 60 mg/kg body weight. OTC levels were determined in plasma and several tissues. Analysis of the plasma drug concentration-time curves following i.v. OTC injection revealed three distinct phases. A three-compartment open model was used to derive pharmacokinetic parameters. Compared to mammals, a very extended final elimination half-life was observed (139.8±38.1 h). Following i.m. OTC administration, C_max was 56.8±10.9μg OTC/ml at 14 h post-injection. The Vd area was 2.1 ± 0.66 1/kg. Extreme differences were observed with respect to bioavailability following i.m. and oral administration; approximately 80 and 0.6%, respectively. Following i.m. injection tissue OTC determinations revealed that the drug was accumulating in pronephros, bone tissue and scales. After 21 days the OTC concentrations were 2.9±0.8, 5.2±0.3 and 4.7±3.1 μg/ ml, respectively. In tissue samples from the dorsal region (muscle), including the injection site, OTC could not be demonstrated at that time. The pharmacokinetic data are discussed in relation to the susceptibility of the immune system of fish for modulation.     

Pharmacokinetics of minocycline in crucian carp (Carassius auratus) after intravenous and oral administration

The pharmacokinetic of minocycline was studied after a single intravenous as well as oral dose (5 mg/kg body weight) in crucian carp (Carassius auratus) reared in freshwater at 10°C. Plasma samples were randomly collected from six fish at each sampling time. Plasma concentrations were determined by high‐performance liquid chromatography and further subjected to noncompartmental analysis. Initial concentration of minocycline just after intravenous administration was calculated as 7.320 μg/ml, while the other parameters after intravenous injection were determined as flows: apparent elimination rate constant (λ_z) of 0.064 per hr, apparent elimination half‐life () of 10.82 hr, total body clearance (Cl) of 142.72 ml/hr/kg, volume of distribution (Vz) of 2,227.38 ml/kg and volume of distribution at steady‐state (Vss) of 1,937.08 ml/kg. While after oral administration, the λ_z, , mean absorption time (MAT), absorption half‐life (t_1/2ka) and bioavailability were determined as 0.059 per hr, 11.74, 5.55, 3.84 hr, and 81.98%, respectively, and the peak concentration was observed as 1.474 ± 0.362 μg/ml at 8 hr. It was shown that minocycline was slowly but relatively completely absorbed, extensively distributed, and slowly eliminated in crucian carp. Based on the ratios of AUC_0–24 hr/MIC90, a minocycline dosage of 5 mg/kg body weight administered intravenously or orally would be only effective to successfully treat crucian carp infected by bacterium with MIC values ≤0.25 μg/ml.     

Pharmacokinetics and tissue residues of marbofloxacin in crucian carp (Carassius auratus) after oral administration

Pharmacokinetics and residue elimination of marbofloxacin (MBF) were studied in crucian carp (Carassius auratus, 250±30 g) kept at two water temperatures of 15 and 25 °C. Marbofloxacin concentrations in plasma and tissues were analysed by means of high‐performance liquid chromatography using an ultraviolet detector. The limits of detection were 0.02 μg mL^?1, 0.02 μg g^?1, 0.025 μg g^?1, 0.02 μg g^?1 and 0.025 μg g^?1 in plasma and muscle, skin, liver and kidney respectively. Fish were administered orally at a single dosage of 10 mg kg^?1 body weight in the PK group. The data were fitted to two‐compartment open models at both temperatures. At 15 °C, the absorption half‐life () and distribution half‐life (t_1/2α) of the drug were 0.36 and 4.48 h respectively. The corresponding values at 25 °C were 0.23 and 0.87 h respectively. The elimination half‐life (t_1/2β) was 50.75 h at 15 °C and 25.05 h at 25 °C. The maximum MBF concentration (C_max) differed little between 15 (6.43 μg mL^?1) and 25 °C (8.36 μg mL^?1). The time to peak concentration was 1.74 h at 15 °C and 0.78 h at 25 °C. The apparent volume of distribution (V_d/F) of MBF was estimated to be 1.36 and 0.87 L kg^?1 at 15 and 25 °C respectively. The area under the concentration–time curve (AUC) was 301.80 μg mL^?1 h at 15 °C and 182.80 μg mL^?1 h at 25 °C. The total clearance of MBF was computed as 0.03 and 0.05 L h^?1 kg^?1 at 15 and 25 °C respectively. After repeated oral administration at a dosage of 10 mg kg^?1 body weight per day for 3 days, the results showed that the elimination half‐lives () of MBF from all tissues at 15 °C were longer than that at 25 °C. Therefore, water temperature is an important factor to be considered when deciding a reasonable withdrawal time.     

土霉素在锯缘青蟹体内的药物代谢和消除规律

采用高效液相色谱法检测土霉素,研究土霉素口灌给药途径下在锯缘青蟹体内的药代动力学。锯缘青蟹口灌给药土霉素50 mg/kg后,其血浆、肌肉和肝胰脏中的药峰浓度分别为16.78±1.98 mg/L、9.39±2.12μg/g和32.12±6.12μg/g,达峰时间分别为4 h、8 h和4 h。血浆中土霉素浓度-时间关系曲线符合一级吸收的二室开放动力学模型。土霉素在锯缘青蟹体内分布广泛,其表观分布容积(Vd)为2.129 L/kg;分布半衰期(t1/2α)和消除半衰期(t1/2β)分别为3.200 h和47.856 h,总体清除率(CLs)为0.063 mL/(kg.h)。肌肉和肝胰脏中土霉素浓度与时间关系的药动学参数采用统计矩原理分析,其消除半衰期(t1/2 z)分别为60.145 h和71.009 h,总体清除率(CLz)分别为0.054 g/(kg.h)和0.037 g/(kg.h)。土霉素在精巢和卵巢中达峰时间分别为8 h和12 h,峰浓度分别为9.83μg/g和10.26μg/g。给药后24 d时,血浆、肌肉、肝胰脏、精巢和卵巢中土霉素含量都已低于0.10μg/g。土霉素在锯缘青蟹体内消除比较缓慢。     

Transferability of oxytetracycline (OTC) from feed to carp muscle and evaluation of the antibiotic effects on antioxidant systems in liver and kidney

Oxytetracycline (OTC) is employed in fish farms to contest or prevent bacterial infections. We simulated an OTC treatment at therapeutic level (75 mg kg^?1) and at higher doses (150, 300 mg kg^?1) for 10 days. A withdrawal period of 10 days was considered for treated carp, carrying out the same chemical and biochemical analyses (total glutathione, superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, glutathione S-transferase and malondialdehyde). The aim was to obtain data related to the carryover in muscle and on variations in the antioxidant indicators in liver and kidney. The OTC residual levels in muscle showed a dose–response relationship. After 10 days of treatment at the recommended dose (75 mg kg^?1), the mean value in muscle was 295 μg kg^?1. After 10 withdrawal days, residues in all treated groups were not entirely eliminated by fish. Residues of recommended 75 mg kg^?1 OTC dose were lower than the maximum permitted by EEC regulation: 100 μg kg^?1. Disturbance in the antioxidant systems in liver and kidney was recorded in (150, 300 mg kg^?1) carp, as well as during the withdrawal period. A lowered superoxide dismutase activity and higher levels of catalase, glutathione peroxidase, glutathione reductase and glutathione were evaluated in liver, while in kidney only higher malondialdehyde and glutathione S-transferase concentrations were recorded for 300 mg kg^?1 dose. The therapeutic OTC dose exerted lower effects, and only in liver, enhancement of GPx and GR activities was recorded. After the withdrawal period, altered antioxidant responses in tissues were restored for all three OTC doses.     

Dietary ginger administration attenuates oxidative stress and immunosuppression caused by oxytetracycline in rainbow trout (Oncorhynchus mykiss)

In the present study, potential ameliorative effects of dietary ginger (GN) were investigated on antioxidant and immune responses of rainbow trout (Oncorhynchus mykiss) during oxytetracycline (OX) administration. As a 2 × 3 factorial design, the fish were orally treated with OX (a daily dose of 100 mg/kg) and GN (either 10 or 20 g/kg diet) for 10 days. Then, blood samples were taken from each treatment to monitor plasma lysozyme, complement (ACH50), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione‐S‐transferase (GST) activities, and reduced glutathione (GSH), malondialdehyde (MDA), total immunoglobulin (Ig) and globulin levels. OX treatment significantly decreased SOD (30%), GPx, (10%) and lysozyme (23%) activities, and GSH (19%) levels; however, it increased GST (16%) activity and MDA (28%) levels. Ten grams GN per kg levels significantly decreased SOD (35%), CAT (13%), GST (20%) and MDA (30%), but increased GSH (30%), lysozyme (48%) and globulin (16%). Twenty grams GN per kg diet significantly decreased SOD (26%) and MDA (17%), but increased lysozyme (31%) levels. Interaction effects of dietary GN and OX were observed on plasma MDA and GPx levels, as 10 g GN per kg diet prevented the OTC‐induced changes in these parameters. Moreover, 20 g GN per kg diet prevented the OX‐induced change in GPx activity and mitigated the MDA elevation by 20%. It is concluded that GN administration at 10 g/kg diet is beneficial in mitigating oxidative stress and immunosuppression of rainbow trout during OX administration.     

建鲤与异育银鲫生长、生化组成和饲料利用的比较

In order to investigate the difference in utilization of diets with different quality in Jian carp（Cyprinus carpio var. Jian）and allogynogenetic silver curcian carp(Carassius auratus gibelio), a 55 d growth trial was conducted and low quality diet (LQ-diet) and high quality diet (HQ diet) were tested. LQ-diet contained 33.91% dietary protein which is mainly from soybean meal while HQ-diet contained 45.59% dietary protein which is mainly from fish meal. The initial average body weights were from 5.58 g to 5.82 g for two fish strains. The trial was carried out in a system consisting of 12 self circulation 320 L tanks. During the experiment, the fish were fed to satiation twice a day (at 9: 00 and 15:00), and uneaten feed was collected 1 h after feeding and dried. Feces were collected twice a day (at 11:00 and 16:45) from the fecal traps and dried at 70 ℃. The results show that feed intake was higher in Jian carp than in allogynogenetic silver curcian carp when fed LQ diet, while there was no significant difference between weight gain, feed conversion efficiency, protein efficiency rate and apparent digestibility. When fed HQ diet, Jian carp showed a lower feed intake, but higher feed conversion efficiency and protein efficiency rate than allogynogenetic silver curcian carp while there was no significant difference in the weight gain and apparent digestibility of both species. For Jian carp, feed intake and protein efficiency rate for the fish fed HQ diet and LQ diet were not significantly different, while the fish fed HQ diet showed higher weight gain, feed conve rsion efficiency and apparent digestibility. For allogynogenetic silver curcian carp, the fish fed HQ diet showed significantly higher feed intake, apparent digestibility, weight gain, feed conversion efficiency and lower protein efficiency rate. For Jian carp, body contents of dry matter, protein, lipid and energy for the fish fed HQ diet and LQ diet were significantly higher. For allogynogenetic silver curcian carp, body contents of dry matter and protein was significantly higher, while body contents of lipid and energy were affected by diet qualities. Compared to Jian carp, allogynogenetic silver curcian carp showed better utilization when fed LQ diet while poorer utilization when fed HQ diet.     

亚甲基蓝在鲤体内消除规律研究

为探究常规用药条件下亚甲基蓝及其代谢物在水产动物体内的分布和消除规律,以鲤(Cyprinuscarpio)为研究对象,以质量浓度为15 mg/L的亚甲基蓝溶液浸泡试验鱼0.5 h后,将其饲养于水族箱中,采用高效液相色谱-质谱串联法检测亚甲基蓝及其代谢物在鲤各组织中的质量浓度,采用PK Solver药动学药效学数据处理软...     

Pharmacokinetics and tissue residues of vancomycin after oral administration in crucian carp

The pharmacokinetics and tissue residues of vancomycin in crucian carp (Carassius auratus) were studied after oral administration of a single dosage of 1 mg/kg body weight at water temperature of 18°C. The drug concentration‐time data were fitted using a two‐compartment open model with first‐order absorption. The vancomycin concentrations in plasma and tissues including muscle, liver and kidney were analyzed by means of liquid chromatography‐tandem mass spectrometry. As a result, the plasma absorption rate constant of vancomycin was 3.75/hr and the time point of maximum concentration in plasma was 3.0 hr respectively. The maximum concentration of vancomycin in plasma was 216.58 μg/L. The distribution half‐life and the elimination half‐life in plasma were 6.69 hr and 190 hr, as well as the absorption half‐life of plasma was 0.19/hr. The area under the plasma concentration‐time curve of plasma was 15.12 mg hr/L. The apparent volume of distribution of plasma was estimated to be 4.45 L/kg and the total body clearance was computed as 0.061 L hr^?1 kg^?1. In addition, the maximum tissue concentrations of vancomycin were in the order of liver > kidney > muscle. To guarantee the safety of tissue samples for consumption, it was suggested that the withdrawal time should not be less than 30 days at 18°C. All these results may provide guidance for future vancomycin treatment in aquaculture.