Photosynthesis of a scots pine shoot: simulation of the irradiance distribution and photosynthesis of a shoot in different radiation fields

Using a model of shoot geometry the distribution of irradiance at the needle surface area of a shoot in different radiation conditions is simulated and shoot photosynthesis calculated. It is shown that the distribution of irradiance is very sensitive to radiation field geometry, affecting the efficiency of the utilization of radiant energy in photosynthesis. As a result, the photosynthetic response of a shoot in natural conditions is, for instance, highly dependent on the proportions of direct and diffuse solar radiation, being higher the greater is the proportion of diffuse radiation.     

The influence of the physical attributes of a spruce shoot on momentum transfer

The drag force on fourteen three-prong spruce shoots (Picea rubens) was measured over a range of wind tunnel velocities. The boundary layer resistance to momentum transfer was found to be influenced by the shoot orientation, inclination, and flexibility. Momentum transfer was also influenced by shoot needle density; however, this influence was a function of the shoot orientation. For wind velocities below about 2.0 m s^?1, these factors did not greatly influence the momentum transfer to the shoots. For wind velocities above about 2.0 m s^?1 the momentum transfer decreased with; (1) decreasing inclination of the shoot to the airflow, (2) decreasing shoot flexibility, and (3) orientating the shoot so that the densely-needled side of the shoot faced the wind. A pair of empirical equations were derived relating momentum transfer to wind velocity.     

The distribution of leaf area,radiation, photosynthesis and transpiration in a Shamouti orange hedgerow orchard. Part II. Photosynthesis,transpiration, and the effect of row shape and direction

The influence of the distribution of radiation in an orange canopy on transpiration and photosynthesis was examined by developing a model of these processes.The leaf energy balance, microclimate relationships and climatic data are combined with radiation, leaf conductance, and leaf carbon uptake models to simulate orchard photosynthesis and transpiration over 2 days. Calculated hourly values of transpiration showed good agreement with measured values of sap flow in the orange orchard.Calculated carbon uptake during the six summer months was 22 kg CO₂ per tree; however, experimental estimates of annual dry matter production yield 55 kg CO₂ per tree. The calculated figure is therefore considerably in error and indicates that present information used in carbon balance modeling of Citrus is inadequate. Even so, it is shown that radiation levels deep in the canopy, where a significant amount of leaf area and transpiration is located, are too low for significant carbon uptake to occur.As an example of the usefulness of the model, the distributions of photosynthesis, transpiration and photosynthetic radiation were simulated in hedgerow canopies of three different shapes following current pruning practices in Israel. The distribution of foliage inside the given hedgerow cross-section was calculated based on the relationship of average measured foliage density to calculated diffuse photosynthetic irradiance in the canopy. The simulation was run for rows oriented north-south and east-west and for climatic conditions of midsummer. The results of the simulation indicated that: (a) The highest photosynthesis in citrus orchards is obtained by covering the largest ground areas possible with a thick canopy, i.e., maximum leaf area index (LAI). Under such conditions most photosynthesis occurs in the upper 1 m of the canopy. (b) Although rows with slanted walls do not have the highest photosynthesis, they allow more light penetration into the canopy and have productive regions on the periphery of the canopy at all heights within the orchard. (c) Whereas row orientation has little influence on total photosynthesis of the orchard, a N-S orientation allows more light penetration into rows with slanted walls and/or wide inter-row alleys, thus reducing spatial variation in the computed photosynthesis. (d) Water use of vertically pruned citrus orchards can be decreased significantly without seriously affecting photosynthesis by reducing canopy height to as low as 3 m.     

油菜光合生产模拟模型

Photosynthetic production is a major determinant of final yield in crop plants. A simulation model was developed for canopy photosynthesis and dry matter accumulation in oilseed rape (Brassica napus L.) based on the ecophysiological processes and using a three-layer radiation balance scheme for calculating the radiation interception and absorption by the layers of flowers, pods, and leaves within the canopy. Gaussian integration method was used to calculate photosynthesis of the pod and leaf layers, and the daily total canopy photosynthesis was determined by the sum of photosynthesis from the two layers of green organs. The effects of physiological age, temperature, nitrogen, and water deficit on maximum photosynthetic rate were quantified. Maintenance and growth respiration were estimated to determine net photosynthetic production. Partition index of the shoot in relation to physiological development time was used to calculate shoot dry matter from plant biomass and shoot biomass loss because of freezing was quantified by temperature effectiveness. Testing of the model for dynamic dry matter accumulation through field experiments of different genotypes, sowing dates, and nitrogen levels showed good fit between the observed and simulated data, with an average root mean square error of 10.9% for shoot dry matter. Thus, the present model appears to be reliable for the prediction of photosynthetic production in oilseed rape.     

Daily photosynthesis totals calculated from solar radiation histograms

Histograms of the number of hours in the day in which the photosynthetic photon flux density (PPFD) of solar radiation was within 5 preset bands, were constructed from readings made with a miniature battery-operated millivolt histogram recorder, connected to a photon sensor. The number of histograms showing a bimodal distribution was much fewer than was expected from a review of the literature, even in a site shaded by trees. This was attributed to smoothing of the data over a whole day, compared with published data which were mostly taken near noon. Bimodal distributions were found in a greenhouse under clear or partly cloudy conditions.The histograms were combined with published data for the instantaneous gross photosynthetic responses to PPFD of two bermudagrass swards, one of low leaf area index (LAI) and highly non-linear response, and the other of high LAI and more linear response. The daily photosynthesis totals of the swards were calculated by summing the products of the number of hours accumulated in each band by the photosynthetic rate in the center of the band.Because of the non-linearity of response, the photosynthesis total in the greenhouse was 75–82% of that calculated for an open site on a clear day, while the daily photon total was only 70% of the open site value. Also, for the same daily photon total, the daily photosynthesis total of each sward on the open site was greater on a day of light clouds (moderate PPFD) than on a day of sunshine alternating with heavy clouds (bimodal histogram of PPFDs). The difference between the two days was greater for the sward with the more non-linear response. These interactions between non-linearity of photosynthetic response and type of radiation histogram were in the expected direction, however, the differences were not very large (+ 10% for the sites and responses studied).The histogram recorder provides an inexpensive and convenient means of obtaining solar radiation data that can be used to calculate daily photosynthesis totals, either from experimental photosynthesis data or from canopy photosynthesis models. Because the response of photosynthesis is non-linear, linear integrals of solar irradiance do not provide sufficient information for such photosynthesis studies.     

The mutual interference of spruce canopy structural elements

The drag force on fourteen three-prong spruce shoots (Picea rubens) has been measured in a wind tunnel in velocities ranging from 0.4 to 4.5 m s^?1. The influence of wind velocity and shoot needle density on momentum transfer and the mutual interference to momentum transfer are evaluated.The influence of the shoot needle density (ranging from 0.6 to 1.7) on momentum transfer is velocity dependent. The shoot needle density does not influence the momentum transfer to the shoot when the wind velocity is less than about 1.5 m s^?1. However, a slight trend of increased momentum transfer to the shoot with decreased shoot needle density was found for wind velocities between 1.5 and 4.5 m s^?1. This trend is not statistically significant.The shelter factor, a measure of the mutual interference to momentum transfer to the shoot structure, is shown to be dependent on the model used to describe the flow around and through the shoot. This suggests that any apparent velocity dependence of the shelter factor may actually be due to a change in flow conditions. It is concluded that: (1) the needles are the vegetative component involved in mutual interference within the shoot for wind speeds between 0.4 and 1.5 ms^?1, and (2) the shoots themselves are the vegetative component involved in mutual interference within a shoot array for wind speeds between 1.5 and 4.5 ms^?1. The shelter factor at the low wind speeds is estimated to be 2.1 The shelter factor at the higher wind speeds should be based on interference between shoots and not between needles within a single shoot.     

Integration of a nonlinear function in a changing environment: Estimating photosynthesis using mean and variance of radiation

If the mean (integral) of a convex or concave function is estimated by evaluating the response function at the mean of the environmental variable, the estimate is necessarily biased. This study computes from empirical radiation data the errors obtained when mean photosynthesis is estimated using mean radiation. Furthermore, three different methods of estimating mean photosynthesis are compared in a case where the second power of the irradiance is also integrated and the variance is thus known. The smallest errors are obtained when the irradiance distribution is approximated by a two-point distribution: the bias is reduced to one-tenth and the root mean square error to one-third compared to the situation when only mean radiation is used. The results indicate that, if accurate estimates are needed for integrals of nonlinear responses, the second power of radiation or any other fluctuating environmental variable should also be measured.     

增强紫外线-B辐射冬小麦产量和光合特性的影响

为了研究紫外线-B（ultraviolet-B UV-B, 280-320nm）辐射增强20%对保护性耕作冬小麦产量及光合特性的影响,在中国南京开展了2 a的田间试验研究。试验采用常规耕作（耕深25 cm）、少耕（耕深10 cm）和免耕（土壤不耕作）3种耕作处理种植冬小麦,采用人工增加紫外辐射的方法模拟UV-B辐射增强。试验测定了冬小麦旗叶的光合速率、光合-光响应曲线、叶绿素质量分数、超氧化物歧化酶（superoxide dismutase, SOD）活性、丙二醛（malonaldehyde, MDA）质量分数和可溶性蛋白质量分数等指标。结果表明：在UV-B辐射增强20%条件下,与常规耕作相比,少耕和免耕2种保护性耕作处理可显著提高冬小麦的产量,分别比常规耕处理作高1632.12和952.15 kg/hm2。从叶片光合生理特性来看,在UV-B辐射增强20%条件下,从冬小麦籽粒灌浆中期开始,少耕和免耕处理旗叶的光合速率、表观量子效率（apparent quantum yield, AQY）、最大净光合速率（Pmax）、叶绿素质量分数、SOD活性、可溶性蛋白质的质量分数显著高于常规耕作处理,MDA质量分数显著低于常规耕作处理,2种处理冬小麦的叶片衰老显著低于常规耕作处理。在UV-B辐射增强20%条件下,少耕和免耕2种保护性耕作处理冬小麦旗叶在籽粒灌浆中期及以后保持高的光合能力和低的衰老程度,是其产量高于常规耕作处理的原因。该文可为制定UV-B辐射增强条件下的冬小麦栽培措施提供参考。     

Modelling Sonchus arvensis root biomass allocation to below-ground shoot and fine root growth

Below-ground (bg) shoot emergence rates of Sonchus arvensis are dependent on temperature and root weight. However, it is unknown to what extent this is due to a root depletion rate that depends on initial root weight, or due to differences in resource allocation to fine root and bg shoot growth. To resolve this, we retrieved data from an experiment in which plants were grown in the dark at constant temperature (4°C, 8°C, and 18°C) and harvested prior to or at shoot emergence. A dynamic mass-balance model, in which biomass of the initial root was allocated to bg shoot and fine root daily growth, and where respiration took place from all tissues, was used. The relative depletion rate of root biomass (RDR; d^?1) and fraction of the depleted biomass allocated to bg shoots (SFRR) were estimated and calibrated to observed biomass. The RDR increased with initial root weight and temperature and SFFR was highest for light roots and lowest for heaviest roots, whereas the rest was allocated to fine root biomass. The length-to-biomass ratio of bg shoots decreased with initial root weight. Under between-year weather variations (2004–2010), the reduction in root biomass during the coldest April–May was simulated to be over 12 days delayed compared with the warmest spring. The influence of biomass allocation on bg shoot elongation of heavier roots was thus stimulated by a larger fraction of root biomass being depleted, but counteracted by a smaller fraction of it allocated into bg shoot elongation, compared with lighter roots. The complexity of shoot emergence based on root depletion estimates may be a reason why predictions based on only an accumulated root weight-specific temperature sum, as proposed by a previous study, are expected to be less uncertain than those based on root depletion estimates.     

光合有效辐射及其传感器研究进展

植物利用约400～700 nm波段的光驱动光合作用,但不同波长的光驱动效率不相同,而且随着植物类型及生长阶段的不同而变化。因此,准确获取被植物捕获并用于驱动光合作用的光辐射成为困扰科学家的难题。当前,光量子传感器被普遍接受并用于评价光合作用潜力,可测量400～700 nm波段的光量子通量密度或光量子通量,其光谱响应函数为直线。该文回顾了经典光合有效辐射（photosynthetically active radiation,PAR）定义的形成过程,介绍了PAR传感器的演化路径,讨论了PAR及其传感器的应用现状。由于测量对象及应用环境的多样化,PAR的定义仍然没有完全统一,且早期研究对光谱响应函数的度量不充分。随着当前人工光照明与植物生长发育相关研究的深入,发现植物光合作用吸收的光波长范围比400～700 nm要宽,不同的光谱能量分布（波长配比,能量配比）、光周期等对光合作用影响显著,并且很难将光辐射对光合作用的影响和光形态效应区分开,因此PAR的定义及其传感器的研发仍处于不断发展中。理想的PAR应该从植物光合作用的角度来定义,未来PAR传感器的光谱响应函数应与植物光合作用的能力曲线相一致,并能依据测量对象及应用需求而调整。与此相适应,未来PAR传感器应向用户可对光谱响应函数编程的方向发展。     

亚磷酸盐作缓释磷肥对黄瓜体内养分吸收和光合特性的影响

本文以黄瓜为材料,采用营养液培养方法,研究了不同亚磷酸盐浓度对黄瓜植株各部位氮、 磷养分含量, 干重及根冠比, 植株氮、 磷总量以及叶片光合特性的影响。结果表明,黄瓜果实、 叶片和根部氮含量随亚磷酸盐浓度的增加而增加,茎部氮含量没有显著差异; 果实和茎部磷含量随亚磷酸盐浓度的增加呈增加趋势,根部磷含量呈降低趋势,叶片磷含量没有显著差异; 亚磷酸盐浓度增加到一定浓度时,各部位干重显著降低,根冠比没有显著差异; 植株氮、 磷总量随亚磷酸盐浓度的增加有下降的趋势; 黄瓜叶片净光合速率（Pn）随亚磷酸盐浓度的增加呈下降趋势。本研究结果显示,随着亚磷酸盐浓度的增加,植株表现出缺磷效应,对植株养分元素的吸收及光合作用产生了不利影响。该文讨论了亚磷酸盐作为缓释磷肥的可行性。     

Vertical variability of spectral ratios in a mature mixed forest stand

The quality and quantity of solar radiation are crucial for growth and competition within forest ecosystems. The spectral waveband between 400 and 700 nm is mainly responsible for photosynthesis and thus for plant growth. Spectral ratios such as red/far red (R/FR) and blue/red (B/R) give important information about the light quality within stands. Changes in R/FR and B/R trigger or inhibit effects such as seed germination, stem growth, dormancy, leaf expansion and flowering. Blue light strongly influences development and growth of plants e.g. an increase of B/R may lead to higher photosynthetic rates per unit leaf area. In addition to spectral properties, a higher fraction of diffuse radiation on overcast days can be used more effectively by plant organs. In this study, the spectral composition and variability of solar radiation were analyzed for different sky conditions and solar angles in six different vertical layers of a mixed European beech and Norway spruce stand in Southern Germany. The results showed lower R/FR ratios in beech than in spruce. Spruce showed markedly higher B/R values in comparison to beech under clear sky conditions. A steep increase of the B/R ratio at the height of the sun crown in both species was observed. Cloud cover had a major effect, elevating R/FR and reducing B/R values compared to clear sky days, due to a higher fraction of diffuse, unattenuated radiation within the canopy. The penetration of blue and red light into the canopy strongly depends on both the diffuse index and solar elevation angle.     

Effect of variations of PAR on CO2 exchange estimation for Scots pine

A set-up with 161 photosynthetically active radiation (PAR) sensors was used to investigate spatio-temporal variations of irradiance for five horizontal arrays within a Scots pine canopy. The measured PAR was converted to CO₂ exchange using of a shoot-scale photosynthetic response curve and the vertical distribution of the needle area. The net ecosystem exchange was simultaneously measured by the eddy covariance technique. The effect of spatial and temporal averaging of the PAR values and the number of sensors were analysed under different conditions as regards cloudiness and the shading by the foliage. In 1/2 h CO₂ exchange values for the entire canopy, a maximum overestimation of 30% resulted from a spatial averaging over horizontal arrays of 2–5 m and occurred under clear-sky conditions and significant foliage shading. Under partly cloudy conditions, the largest overestimation occurred for a case of little shading and the inaccuracy resulting from 1/2 h temporal averaging exceeded that of spatial averaging.     

Effects of lead (PaCl2) on photosynthesis and respiration of the bladder wrack,Fucus vesiculosus,in relation to different salinities

The photosynthetic and respiratoric capacity of Fucus vesiculosus grown at three different levels of salinity (4.5, 8 and 20‰) was studied in the presence of different concentrations of lead (5, 10, 15, 20, 25, and 30 mg PbCl₂ L^?1). The studies were performed by using a light-pipette, which has a precisely defined light source of photosynthetic active radiation (PAR, 400–700 nm). The photosynthetic and respiratoric capacity of the algae were measured when exposed to light pulses with increasing irradiance and after 24 hr in seawater with the addition of lead. The results indicate that the photosynthetic capacity of F. visiculosus from 20‰ is more sensitive to lead compared to those from 8 and 4.5‰. The photosynthetic capacity was reduced by 69% at 30 mg PbCl₂ L^?1 compared to the control alga. The photosynthetic capacity of F. vesiculosus from 8 and 4.5‰ was reduced by 38 and 15%. The respiratoric capacity of F. vesiculosus from 20‰ was reduced by 41% at 30 mg PbCl₂ L^?1. However, the respiratoric capacity of the algae from 4.5‰ increased by 55% at the same concentration of PbCl₂. The results indicate that lead showed inhibitory effects on photosynthesis but a stimulatory effect on respiration of F. versiculosus grown at low levels of salinity.

     

Effect of crude oil on gas exchange functions of Juncus roemerianus and Spartina alterniflora

The effect of crude oil on gas exchange functions of Juncus roemerianus and Spartina alterniflora, two important U.S. Gulf Coast plant species, were examined. Plants were exposed to petroleum hydrocarbons (Mexican Sour type) mixed with water at 4.4 mL L^?1 (2 L m^?2). Coating entire leaf with oil resulted in cessation of photosynthetic activity. Partial leaf exposure to oil resulted in net photosynthesis decrease in both species shortly after exposure. Net photosynthesis remained within 71 and 94% of control plants in J. roemerianus during the first four weeks of the experiment. In S. alterniflora, photosynthetic rates averaged between 53 to 80% of control plants during the same period. Four weeks following the partial oiling, net photosynthesis began to improve in both species. Partial oil coverage of leaves of both species resulted in reduction in normal photosynthetic activity with no lethal effects. By the end of the laboratory study, there was no significant reduction in production of new shoots or the overall growth of either species.     

Photosynthetic physiology of eucalypts along a sub-continental rainfall gradient in northern Australia

Leaf-level photosynthetic parameters of species in the closely related genera Eucalyptus and Corymbia were assessed along a strong rainfall gradient in northern Australia. Both instantaneous gas exchange measurements and leaf carbon isotope discrimination indicated little variation in intercellular CO₂ concentrations during photosynthesis (c_i) in response to a decrease in mean annual precipitation from ∼1700 mm to ∼300 mm. Correlation between stomatal conductance and photosynthetic capacity contributed toward the maintenance of relatively constant c_i among the sampled leaves, when assessed at ambient CO₂ concentration and photon irradiance similar to full sunlight. Leaf mass per area was the most plastic leaf trait along the rainfall gradient, showing a linear increase in response to decreasing mean annual precipitation. The maximum Rubisco carboxylation velocity, V_cmax, expressed on a leaf-area basis, showed a modest increase in response to decreasing rainfall. This modest increase in V_cmax was associated with the strongly expressed increase in leaf mass per area. These results suggest that variation in ecosystem-level gas exchange during the dry season in north-Australian savannas will likely be dominated by changes in leaf area index in response to increasing aridity, rather than by changes in photosynthetic performance per unit leaf area.     

Total reflected solar radiation calculated from multi-band sensor data

Reflected solar radiation is a significant term in the net radiation equation and is the one most strongly affected by surface conditions. The evaluation of net radiation over a heterogeneous area requires a detailed knowledge of the areal distribution of the reflected solar radiation. Remote sensing offers a means to obtain this areal distribution, provided that the total reflected solar spectrum can be estimated from discrete band multispectral radiometric data. A radiative transfer model was used to calculate the irradiance at the earth's surface for a number of atmospheric scattering and adsorption conditions. Response functions of two multiband radiometers were used to obtain the partial spectrum/total spectrum (P/T) ratio for each radiometer at each atmospheric condition. It was found that the P/T ratio was essentially independent of atmospheric scattering and only mildly dependent on water vapor absorption. Reflectance spectral distributions for 14 different surface conditions (bare soil to full green canopy) were used along with the irradiance data to determine the P/T ratio for reflected solar radiation. Multispectral data, with the appropriate P/T ratio, were used to calculate the total incoming radiation and the total reflected radiation from a wheat canopy. The calculated data differed from wide band pyranometer data by about 5%. It was concluded that both total incoming and reflected solar radiation can be evaluated from multispectral radiometric data. This development is a step towards regional net radiation maps, and possibly regional evapotranspiration maps.     

新疆棉田小区域碳变化量研究

为研究膜下滴灌条件下棉田小区域碳变化量,在棉花生长期内采用Li-8100A土壤碳通量自动测定仪和LCpro+光合仪连续观测不同样地土壤呼吸速率和净光合速率。结果表明:不同样地的土壤呼吸速率日变化均表现为单峰型曲线,峰值出现在16:00;而棉田净光合速率日变化则表现为单双峰曲线交替变化规律。两者月变化均呈现先升高后降低的趋势,7月土壤呼吸速率和净光合速率达到最大值。不同样地间土壤呼吸速率和净光合速率存在差异,土壤呼吸速率表现为垄间>垄上>裸地,净光合速率表现为垄间>垄上。棉田小区域在棉花整个生育期内均表现为碳汇,6-9月棉田净固碳总量为2 203.7 C g/m^2,光合作用固碳量为土壤呼吸排碳量的2.67倍。