Yield Stability of Winter Oil-Seed Rape (Brassica napus L.) as Affected by Stand Establishment and Nitrogen Fertilization

The effects of stand establishment and nitrogen fertilizer on yield and yield stability of winter oil-seed rape ( Brassica napus L.) were investigated in two field experiments in north eastern Germany (Mecklenburg-Vorpommern). During six years of testing from 1984/85 to 1989/90 three sowing dates (10 Aug, 20 Aug, 5 Sept) as combined with three plant densities (60, 100, 140 plants/m² in autumn) were compared. Although seed yield did not respond to the main effects of these treatments, the two-way classification resulted in significant differences from the average yield. August sowing combined with lower plant densities caused comparably high yields, while delayed sowing was somewhat effective only with higher plant density. Highest yield stability was achieved at early sowing when combined with lower plant densities. From 1986/87 to 1988/89 nitrogen top-dressings were applied during early March, at start of stem extension and shortly before start of flowering at five locations. 200 kg N/ha in one dose resulted in lowest yield performance and enhanced instability. Contrastingly, the 240 kg N/ha treatment advanced yield stability regardless of split regimes. Nevertheless, split application to 100 kg N/ha + 50 kg N/ha + 50 kg N/ha also guaranteed that high yield performance and optimum yield stability were reached simultaneously and, that the total nitrogen input could be limited to 200 kg N/ha.     

The Effect of Different Preceding Crops on the Development, Growth and Yield of Winter Barley

Reliable estimations of the yield response of winter barley to different preceding crops are necessary for the design of crop rotations.
The grain yield and yield components of winter barley (cv. Tapir ) following either rapeseed, oats, wheat or barley were determined in five years of field experiments on a sandy loam (Luvisol) at the Hohenschulen experimental station near Kiel, Germany, F.R. The growth, development and incidence of take-all ( Gaeumannomyces graminis var. tritici ) was measured in a total of three years. On average over the five years barley grown after oats yielded 0.8 t per ha (11 %) more than barley following wheat which was mainly due to a higher number of ears per m². Barley following either oats or rapeseed produced a higher dry weight and a larger number of tillers per m² compared with barley grown after wheat or barley. This effect was already-present at the sampling date before winter. Take-all ratings were constantly higher in barley following a susceptible crop, but only reached a severe level late in the season and therefore could not explain the observed differences in growth, development and subsequently grain yield. Since no other pathogens affected the development other non-pathogenic causes must be considered as main causes for the described observations and yield differences.     

前作、土壤有效氮及烤烟施氮对烟叶产量和产值的影响

针对玉溪田烟前作蔬菜面积增加及其肥料用量较大,对后作烤烟的施肥及烟叶产质量产生明显影响的实际问题,于2004-2006年采用大区试验方法开展相关试验研究。结果表明：在同一土壤有效N水平内及施N量基本相近的条件下,小麦及油菜、蚕豆、大蒜及叶菜类、菜豌豆等不同前作的烤烟产量、产值及中上等烟比例有明显差异。烤烟施N量,除考虑土壤有效N含量外,还要考虑前作;烤烟前作为大蒜、叶菜类、菜豌豆等施氮量较大的田块,要严格控制烤烟的施氮量;提出了土壤有效N及不同前作与烤烟施N量的关系表,丰富和完善了烤烟科学施肥的内涵。     

Effect of Timing and Nitrogen Fertilizer Application on Winter Oilseed Rape (Brassica napus L.). I. Growth Dynamics and Seed Yield

The field experiments conducted on the grey‐brown podzolic soil in the four growing seasons (1998–2001) at Krzeslice Farm, central‐western Poland comprised seven fertilization variants: 80_NF + 80_CAN; 80_CAN + 80_CAN; 80_AN + 80_AN; 80_NF + 50_CAN + 30_CN; 80_CAN + 50_CAN +30_CN; 80_AN + 50_AN + 30_CN (where NF – nitrofos NPK; CAN – calcium‐ammonium nitrate; AN – ammonium nitrate; CN – calcium nitrate) and control (without N) applied in split rates at the beginning of spring regrowth (80 kg N ha^?1), stem elongation (80 or 50) and flower buds visible stages (30). The yielding effect of tested fertilization variants was significant in comparison with the control (2.24 t ha^?1). The highest mean seed yield (3.64 t ha^?1) was collected from 80_AN + 80_AN and 80_CAN + 80_CAN variants. Mean values of 4 years indicate that the second N rate division (80 + 50 + 30) decreased yield, although not significantly in comparison with these two N treatments. Plants grown on these treatments have developed different patterns of growth to yield the seeds. These patterns were characterized by very high crop growth rate during flowering (above 21 g m^?2 day^?1) and negative at maturation (down to ?2.5 g m^?2 day^?1). Plants fertilized with ammonium nitrate (80_AN + 80_AN) reached maximum growth rate earlier (65 days), which lasted longer (20 days) than plants fertilized with calcium‐ammonium nitrate (71 days lasting 17.5 days). Plants grown on the control treatment reached the highest crop growth rate within 79 days (14.8 g m^?2 day^?1), which lasted 15 days.     

油菜新品种中双11号高产栽培优化技术研究

为研究种植密度、施氮量对油菜新品种中双11号产量的影响,采用二次饱和D-最优设计方案,建立各因素与中双11号产量及产量各构成因素间的数学模型。结果表明,各因素对产量的影响大小是施氮量>种植密度;在直播种植模式下,中双11号油菜品种最高产量可达到2 889.76kg/hm2的栽培模式为纯氮施用量为209.25kg/hm2、密度为52.5万株/hm2。同时根据试验结果对产量高于2 000kg/hm2目标产量的栽培措施进行了优化。     

不同冬季覆盖作物轮作对农田土壤碳氮影响

风蚀是干旱区农田生态系统中土壤质量降低的关键因素,冬季作物覆盖可有效减少农田的土壤风蚀。通过探究河西灌区不同冬季覆盖作物轮作复种绿肥对农田土壤碳氮影响,以期为构建合理的周年覆盖轮作模式提供理论依据。本研究在热量一熟有余两熟不足的河西灌区春小麦种植区把冬小麦、冬油菜两种冬季覆盖作物和绿肥还田处理嵌套种植形成：（1）春小麦—冬油菜—箭筈豌豆(WCP)、（2）春小麦—冬小麦—箭筈豌豆(WWP)、（3）春小麦—箭筈豌豆(WP)、(4)春小麦—春小麦(W,CK)不同种植模式,在360 kg/hm² (N2)、270 kg/hm² (N1)、0 kg/hm² (N0) 3个施氮水平下,研究不同轮作模式对农田土壤碳、氮含量的提升效应。结果表明：在同一种植模式下土壤有机碳、土壤可溶性有机碳、热提取态有机碳、硝态氮、氨态氮、微生物量碳氮含量随施氮量的增加而增加,但在氮肥减量(N1)的条件下,与常规施氮(N2)相比较WCP轮作模式土壤有机碳、土壤可溶性有机碳、热提取态有机碳含量及微生物量氮无显著降低。相同施氮条件下,轮作模式间差异不显著,但与CK间差异显著;其中,0~10 cm土层,WCP轮作模式土壤有机碳、土壤可溶性有机碳、土壤热提取态有机碳、硝态氮、氨态氮、微生物量碳氮含量平均较CK提高5.42%、9.78%、10.96%、20.51%、15.76%、18.94%;10~30 cm土层,提高9.54%、7.06%、12.99%、20.12%、16.51%、18.16%。因此,春小麦轮作冬油菜复种绿肥模式在氮肥减量条件下仍对农田土壤碳氮有明显的提升效应,为河西灌区良好的周年覆盖作物轮作模式。     

长江流域直播冬油菜氮磷钾硼肥施用效果

冬油菜直播种植的推广应用对提高我国油菜籽总产量和保障食用油安全有重要意义。2009-2010年度在长江流域6个冬油菜主产省布置36个大田试验,研究当前生产条件下我国直播冬油菜的氮磷钾硼肥施用效果和肥料利用率,比较农民习惯施肥与推荐施肥的差异,并与移栽油菜施肥效果进行对比,以探讨直播冬油菜的施肥管理措施。结果显示,在推荐施肥量和施肥方法条件下,氮磷钾硼肥配合施用(NPKB)处理直播冬油菜的油菜籽产量和产值平均分别为2001 kghm^–2和8205元hm^–2,显著高于农民习惯施肥(FFP)处理,施用效果为N > P > B > K。NPKB配合施用显著促进直播冬油菜的养分吸收,N、P、K累积量分别为104.2、20.4和160.2 kg hm^–2。NPKB配施条件下,直播冬油菜的氮、磷、钾肥表观利用率平均分别为35.8%、22.3%和45.9%,明显高于农民习惯施肥的20.8%、7.2%和28.0%。说明合理施肥对直播冬油菜生产有重要作用,现阶段应积极推行氮磷钾硼肥配施技术,并根据生长发育特点和养分吸收规律调整肥料的施用时期和比例。     

硼对甘蓝型双低春油菜产量和品质的影响

旨在阐明硼对甘蓝型双低春油菜产量和品质形成的影响机理,明确甘蓝型双低春油菜产量和品质形成与施硼量的关系。试验以甘蓝型双低春油菜"青油14"为供试材料,采用单因素五水平随机区组设计,研究了不同施硼量对甘蓝型双低春油菜产量和品质形成的影响。结果表明:甘蓝型双低春油菜单株角果数与施硼量之间呈显著线性关系,而每角果粒数则与施硼量呈二次曲线关系,适量施硼可以协调每株角果数与每角果粒数的关系,有利于提高产量。籽粒产量与每角果粒数呈极显著正相关性(r=0.982 8**),与单株一次有效分枝数和千粒重也呈一定的正相关性,而与单株角果数则呈一定的负相关性。适量施用硼肥,可明显提高甘蓝型双低春油菜的籽粒产量和产油量,增加籽粒粗脂肪和粗蛋白质含量,而降低硫苷含量,进而改善品质。籽粒产量、产油量与施硼量之间均呈二次曲线关系,施硼量分别为17.84 kg/hm2和19.42 kg/hm2时,籽粒产量和产油量分别达到理论最大值3 154.18 kg/hm2和1 344.61kg/hm2,施硼过量,籽粒产量、产油量均下降。粗脂肪含量与施硼量之间呈单峰曲线关系,粗脂肪含量达到最大值(45.3%)时的施硼量为24.62 kg/hm2。硫苷含量与施硼量则呈开口向上的二次曲线关系,施硼量为19.42 kg/hm2时,硫苷含量为10.29 mg/g(≤30 mg/g)。试验综合考虑甘蓝型双低春油菜的籽粒产量、产油量及籽粒综合品质,提出适宜的施硼量(含硼11%)以19.42 kg/hm2为宜。     

Effect of Timing and Nitrogen Fertilizer Application on Winter Oilseed Rape (Brassica napus L.). II. Nitrogen Uptake Dynamics and Fertilizer Efficiency

Field experiments were carried out on grey‐brown podzolic soil in the four consecutive growing seasons (1998–2001) at Krzeslice Farm, central‐western Poland. The effect of seven N fertilization treatments (in kg N ha^?1): 80_NF + 80_CAN; 80_NF + 50_CAN + 30_CN; 80_CAN + 80_CAN; 80_CAN + 80_CAN + 30_CN; 80_AN + 80_AN; 80_AN + 50_AN + 30_CN, where, NF – nitrofos NPK, CAN – calcium‐ammonium nitrate, AN – ammonium nitrate, CN – calcium nitrate and control (without N) on N uptake dynamics and N efficiency was studied. Mineral fertilizers were applied at the start of spring regrowth, beginning of stem elongation and at the flower‐bud‐visibility stage. The study revealed two distinct strategies of oilseed rape plants’ adaptation to timing and N fertilizer application sequences. Both strategies based on nitrogen uptake rate (NUR), were analysed at different plant growth stages. Ammonium nitrate (AN) applied in the two‐split system gave the highest NUR (387 mg m^?2 day^?1) during stem elongation (for comparison, a value of 166 mg m^?2 day^?1 was obtained in the control). In the case of calcium‐ammonium nitrate (CAN), a moderate level of NUR was obtained (304 mg m^?2 day^?1) but N uptake lasted 12 days longer compared with the AN treatment. Hence, N accumulation in leaves at the end of flowering explained about 81 % of yield variability. The second adaptation strategy was attributed to the three‐split N treatment. Plants fertilized with AN and CAN fertilizers showed an inconsistent pattern of NUR with time. Nitrogen accumulation in stems at the beginning of maturity, explained 69 % of yield variability. Nitrogen‐use efficiency did not show any response to N treatments.     

On Harvest Indices of Winter Oilseed Rape (Brassica napus L.)

Ten quite different European winter rapeseed cultivars were grown in a 3-year field trial at one location. The traits grain yield and biomass were measured based on single plant measurements (first year) or on plot basis (second and third year). Individual harvest indices were calculated.
Besides a general discussion of the experimental results for this collection of winter rapeseed cultivars the main aim of the present investigations was an examination whether these experimental results are in agreement with the theoretical conclusions on harvest indices which have been published in this journal in three preceding publications.
Hence the investigations of this paper are divided into the three chapters: 1) Calculation of mean harvest indices, 2) Variability of harvest indices and 3) Correlations among harvest index, grain yield and biomass. In all three chapters the agreement between the empirically obtained and the theoretically expected values is quite good.     

The Effect of Preceding Crop and Pre-Sprouting on Crop Growth, N Use and Tuber Yield of Maincrop Potatoes for Processing Under Conditions of N Stress

Factorial field trials were carried out on an experimental farm near Kassel, Germany, in two consecutive seasons (2003 and 2004) in order to examine the impact of leguminous and cereal preceding crops (i) on soil N availability under subsequent potatoes and (ii) the effect of preceding crop and pre‐sprouting of seed tubers on crop development, N uptake, N utilization efficiency and total and size‐graded tuber yields relevant for processing into either crisps or French fries. In addition, an approach to analyse complex field experiments using mixed models is discussed. Soil mineralized nitrate‐N at emergence of the potato crop was affected by the preceding crop and was highest when potatoes followed peas, while the short‐term alfalfa/grass/clover ley appeared too sensitive to environmental conditions in the preceding cropping season, and its efficiency in terms of N supply may be hard to predict. Pre‐sprouting advanced crop development and dry matter accumulation of the canopy, translocation of assimilates and N from canopy into tubers and allowed an increased N utilization efficiency. The positive effect of pre‐sprouting on total tuber yield was compensated up to the final harvest, but a higher percentage of marketable tuber yield for French fries (>50 mm) was found independently of the date of harvest. An increasing N supply (after peas and a following catch crop) may be efficient in terms of higher yields of the marketable size‐grades (40–65 mm) for crisps, but increase oversized tuber yields in seasons not affected by Phytophthora infestans. Average tuber fresh weight responded consistently and positively to seed‐tuber preparation (pre‐sprouting), cultivar (cv. Agria) and an increased N supply (after peas).     

不同前茬对烤烟根际土壤微生物的影响

以烤烟‘K326’为材料,在石林和寻甸2个不同气候生态烟区,通过田间试验研究1年2熟条件下油菜、小麦、大麦、绿肥和冬闲5种前茬对植烟土壤根际微生物量的影响。研究结果表明：在2个生态区不同前茬对烟株根际土壤微生物的影响各不相同;前茬绿肥、小麦能增加烤烟根际土壤中后期细菌、放线菌数量,降低根际真菌量;大麦、冬闲前茬到烟株打顶时,根际土壤真菌数量明显大于油菜、小麦及绿肥前茬;在2个生态气候区,前茬种植油菜对烟田根际土壤真菌群落影响不明显,大麦与绿肥前茬下植烟土壤根际微生物量明显大于其他几种前茬类型,其中又以绿肥好于大麦。     

Inheritance of Total and Individual Glucosinolate Contents in Seeds of Winter Oilseed Rape (Brassica napus L.)

Genetic analyses were carried out with B. napus lines that differed in total content of individual glucosinolates (GSLs), as well as in their profile. Inheritance of total GSL content was studied with a complete F₁ diallel mating of eight doubled haploid (DH) lines (5–120/μmol GSL/g dry seed) as well as with the segregating populations of two crosses between DH lines. Most of the genetic variability was caused by gca effects; heterosis was not important; heritability of GSL content was high (h²_b, = 0.95, h²_n= 0.87). For GSL contents below 20/μmol/g seed, heritability values reached h²_b, = 0.69 and h²_n, = 0.66. Low total GSL content was found to be controlled by 4–5 recessive genes with additive gene action. Alkenyl GSL profiles studied in F₂ from resynthesized rapeseed lines were determined by four loci, two that are responsible for the elongation of the butenyl to pentenyl GSLs and two that are responsible for hydroxylation of alkenyl GSLs. Initial genetic studies of indolyl GSL contents from 0.1–4.5/μmol/g show that two or three genes may be involved. The frequency distribution of F₂ phenotypes was virtually continuous due to both segregational and environmental variation. Genotypes with low alkenyl and low indolyl GSL contents were selected after crossing parents with low alkenyl/high indolyl and high alkenyl/low indolyl GSL content. The alkenyl GSL profiles are discussed with regard to the parental diploid species B. oleracea and B. campestris.     

Effect of Phosphorus Deficiency on Reflectance and Chlorophyll Fluorescence of Cotyledons of Oilseed Rape (Brassica napus L.)

The spectroscopic changes in reflectance and fluorescence caused by phosphorus (P) starvation in Brassica napus L. young plants were evaluated. P deficiency produced an important decrease in reflectance values between 500 and 650 nm for both intact leaves and cotyledons. Furthermore, cotyledons under P deficiency showed a Chl-F ratio in the red/far-red region (F_red/F_far-red) lower than that of non-stressed plants (1.91 and 2.89 respectively). As minimal differences in F_red/F_far-red were detected in leaves, P deficiencies may be better perceived by measuring changes in Chl-F emission in cotyledons than in leaves. Stressed cotyledons also showed different emission spectra in the blue green (maxima at 470 and 560 nm) from those of non-stressed cotyledons. The results are explained in terms of higher anthocyanin and chlorophyll contents and of damage to photosystem II. We evaluate that measuring variations in fluorescence and reflectance data may be useful to detect early damages induced by P stress.     

Effects of Nonregular Spatial Distribution of Plants on Yield per Area: A Theoretical Approach with Applications to Winter Oilseed Rape (Brassica napus L.)

In this study a stochastic approach, based on several simplifying assumptions, is developed that allows an estimation of the effects of nonregular spatial patterns of the distribution of individual plants on yield per area. In this approach, two random variables were attached to each individual plant: single plant yield and individual space per plant. The latter can be estimated, for example, by the area of Thiessen polygons. Yield per area is calculated theoretically by the expectation (=mean) of the ratio between individual yield and area. Based on the logarithmic relationship between single plant yield and individual space per plant, yield per area can be broken down into three additive terms: the first term depends only on the mean of individual plant areas, while the second depends on their mean and variance simultaneously. This second term is the product of the variance and a factor which depends only on the mean. The third term is a function of the mean and of higher order (≥ 3) central moments of a fractional linear transformation of individual plant area. Finally, these theoretical concepts were applied to 17 experimental data sets of three cultivars of winter oilseed rape (Brassica napus L.) with measurements for single plant yields and individual areas for the Thiessen polygon tesselations. Since yield per area is theoretically defined by the expectation of the ratio between individual yield and area, it is estimated by the arithmetic mean of the individual yield/area‐ratios. The agreement between this estimate and the sum of the estimated terms from the aforementioned additive decomposition of yield per area is particularly good. For the 17 data sets, percentages for these additive terms of approximately 71.4 % up to 98.4 % (mean: 89.0 %) for the first term, 1.6 % up to 20.0 % (mean: 9.8 %) for the second term and 0 % up to 8.7 % (mean: 1.2 %) for the third term are obtained. As a consequence it may be concluded, that yield per area can be explained mainly by dependencies on the means of individual plant areas while the variance of individual plant spaces is of only minor importance. The effect of the third term is insignificant. These results clearly indicate an answer to the main issue raised in the paper, namely the importance of seeding density as opposed to seeding accuracy/uniformity: nonuniformity is of limited influence and seeding density is the main correlate of yield/area. Seed placement accuracy of seeding technology therefore plays an only minor role.     

Effect of Completion of Vernalization on Generative Development of Winter Rape (Brassica napus L. var. oleifera) Plants

Germinating seeds and young plants of winter rape var. Górczañski were vernalized for 56–63 days under conditions of 9-hour day, at the temperature 2 and 5 °C and in continuous darkness at the temperature 2 °C. After vernalization the plants grew under conditions enabling to complete vernalization: in a glass-house at the temperature day/night 15/10 °C and in semi natural conditions of open vegetation hall in the period from June till August. After sub-optimal vernalization further growth of the plants at lowered temperature increased its effectiveness (completion of vernalization). Depending on the degree of the vernalization of the plants the completion of their vernalization was both obligatory, i.e. conditioning the acquisition of the ability of generative development, and facultative i.e. accelerating this development. It has been demonstrated that the population of plants of the examined variety is strongly differentiated not only with respect of vernalization requirements in the particular plants, but also what regards the effectiveness of vernalization completion. New observations have been made indicating that the mechanisms controlling the successive phases of generative development, i.e. phase of forming flower buds and the flowering phase are not identical which may be interpreted as indicating that the "flowering factor" is polymorphous.     

缓释型配方肥在晚稻套播油菜上的施用效果研究

油菜套播是利用双季稻区冬闲田的有效手段,为探究缓释型配方肥（N-P₂O₅-K₂O：25-7-8,及5%的中微量元素）在晚稻套播油菜上的施用效果,设置一次性基施缓释型配方肥与分次施常规肥效果对比试验和配方肥施用量试验,调查油菜产量形成、肥料利用率及经济效益。结果表明,在减少肥料养分投入且一次性基施的情况下,缓释型配方肥仍能达到甚至优于常规肥分次施用的效果。菜籽产量与缓释型配方肥用量呈线性加平台关系,适宜施肥量可保证较大的收获密度并产生较多的每株角果数和每角粒数,同时维持较高的肥料利用率,从而提高籽粒产量和经济效益。根据本试验结果推算出晚稻套播油菜的目标产量为1 500~1 800kg/hm²时,缓释型配方肥的适宜用量为645~715kg/hm²。     

甘蓝型油菜抗倒伏性及农艺性状研究

以5个高产优质甘蓝型油菜品种为材料,分析了不同密度下根系及茎秆生物学特性、株型结构、农艺性状及这些性状与抗倒伏性的关系。结果表明,在不同密度下中双9号抗倒伏性强;密度与抗倒伏指数呈极显著负相关(r=-0.944),与倒伏系数呈极显著正相关(r=0.842)。茎秆拉力、根颈粗、根鲜(干)重、茎秆鲜(干)重、重心高度/株高、抗折力、茎壁厚、一次有效分枝数、单株角果数、每角粒数、千粒重、单株产量与抗倒伏指数呈极显著正相关,茎秆拉力、根茎粗、根鲜(干)重、茎秆鲜(干)重、抗折力、一次分枝长度、单株角果数、每角粒数、单株产量等与倒伏系数呈极显著负相关。应将根颈粗、茎秆鲜(干)重、抗折力、重心高度/株高、分枝部位、抗倒伏指数等作为评价油菜品种抗倒伏性的主要指标。     

Growth and Yield Responses to Plant Density and Stage of Transplanting in Winter Oilseed Rape (Brassica napus L.)

Growth and yield responses to plant density (6.75 × 10⁴, 9.75 × 10⁴ and 12.75 × 10⁴ plants ha^–1) and stage of transplanting (30, 35 and 40 days after sowing) of winter oilseed rape cultivar HO 605 were investigated in two field trials in the 1996/97 and 1997/98 growing seasons at Zhejiang University Farm, Huajiachi Campus, China. Results revealed a progressive decrease in leaf area per plant in response to increasing plant density and delayed transplanting, though leaf area m^–2 and leaf area index were higher in high-density plants. Number of effective branches and pod per branch decreased with increasing plant density and delayed transplanting. There were no significant differences in the mean seed weight among treatments. Although the average number of seeds per pod was significantly lower for high-density plants and delayed transplanting, the economically highest seed yields were realized in relatively high-density plants. Seed oil content was negatively affected by increasing plant density, but no significant differences were observed with delayed transplanting. The highest seed yields of 1730.7 and 1748.1 kg ha^–1 with no significant differences were observed for plant densities of 9.75 × 10⁴ and 12.75 × 10⁴ plants ha^–1, respectively, transplanted at 35 and 30 days after sowing.