Dietary mitigation of enteric methane emissions from ruminants: A review of plant tannin mitigation options

Methane gas from livestock production activities is a significant source of greenhouse gas (GHG) emissions which have been shown to influence climate change. New technologies offer a potential to manipulate the rumen biome through genetic selection reducing CH₄ production. Methane production may also be mitigated to varying degrees by various dietary intervention strategies. Strategies to reduce GHG emissions need to be developed which increase ruminant production efficiency whereas reducing production of CH₄ from cattle, sheep, and goats. Methane emissions may be efficiently mitigated by manipulation of natural ruminal microbiota with various dietary interventions and animal production efficiency improved. Although some CH₄ abatement strategies have shown efficacy in vivo, more research is required to make any of these approaches pertinent to modern animal production systems. The objective of this review is to explain how anti-methanogenic compounds (e.g., plant tannins) affect ruminal microbiota, reduce CH₄ emission, and the effects on host responses. Thus, this review provides information relevant to understanding the impact of tannins on methanogenesis, which may provide a cost-effective means to reduce enteric CH₄ production and the influence of ruminant animals on global GHG emissions.     

The role of seaweed as a potential dietary supplementation for enteric methane mitigation in ruminants: Challenges and opportunities

Seaweeds are macroalgae, which can be of many different morphologies, sizes, colors, and chemical profiles. They include brown, red, and green seaweeds. Brown seaweeds have been more investigated and exploited in comparison to other seaweed types for their use in animal feeding studies due to their large sizes and ease of harvesting. Recent in vitro and in vivo studies suggest that plant secondary compound-containing seaweeds (e.g., halogenated compounds, phlorotannins, etc.) have the potential to mitigate enteric methane (CH₄) emissions from ruminants when added to the diets of beef and dairy cattle. Red seaweeds including Asparagopsis spp. are rich in crude protein and halogenated compounds compared to brown and green seaweeds. When halogenated-containing red seaweeds are used as the active ingredient in ruminant diets, bromoform concentration can be used as an indicator of anti-methanogenic properties. Phlorotannin-containing brown seaweed has also the potential to decrease CH₄ production. However, numerous studies examined the possible anti-methanogenic effects of marine seaweeds with inconsistent results. This work reviews existing data associated with seaweeds and in vitro and in vivo rumen fermentation, animal performance, and enteric CH₄ emissions in ruminants. Increased understanding of the seaweed supplementation related to rumen fermentation and its effect on animal performance and CH₄ emissions in ruminants may lead to novel strategies aimed at reducing greenhouse gas emissions while improving animal productivity.     

Factors affecting methane production and mitigation in ruminants

Methane (CH₄) is the second most important greenhouse gas (GHG) and that emitted from enteric fermentation in livestock is the single largest source of emissions in Japan. Many factors influence ruminant CH₄ production, including level of intake, type and quality of feeds and environmental temperature. The objectives of this review are to identify the factors affecting CH₄ production in ruminants, to examine technologies for the mitigation of CH₄ emissions from ruminants, and to identify areas requiring further research. The following equation for CH₄ prediction was formulated using only dry matter intake (DMI) and has been adopted in Japan to estimate emissions from ruminant livestock for the National GHG Inventory Report: Y = −17.766 + 42.793X − 0.849X², where Y is CH₄ production (L/day) and X is DMI (kg/day). Technologies for the mitigation of CH₄ emissions from ruminants include increasing productivity by improving nutritional management, the manipulation of ruminal fermentation by changing feed composition, the addition of CH₄ inhibitors, and defaunation. Considering the importance of ruminant livestock, it is essential to establish economically feasible ways of reducing ruminant CH₄ production while improving productivity; it is therefore critical to conduct a full system analysis to select the best combination of approaches or new technologies to be applied under long-term field conditions.     

Meta-analysis quantifying the potential of dietary additives and rumen modifiers for methane mitigation in ruminant production systems

Increasingly countries are seeking to reduce emission of greenhouse gases from the agricultural industries, and livestock production in particular, as part of their climate change management. While many reviews update progress in mitigation research, a quantitative assessment of the efficacy and performance-consequences of nutritional strategies to mitigate enteric methane (CH₄) emissions from ruminants has been lacking. A meta-analysis was conducted based on 108 refereed papers from recent animal studies (2000–2020) to report effects on CH₄ production, CH₄ yield and CH₄ emission intensity from 8 dietary interventions. The interventions (oils, microalgae, nitrate, ionophores, protozoal control, phytochemicals, essential oils and 3-nitrooxypropanol). Of these, macroalgae and 3-nitrooxypropanol showed greatest efficacy in reducing CH₄ yield (g CH₄/kg of dry matter intake) at the doses trialled. The confidence intervals derived for the mitigation efficacies could be applied to estimate the potential to reduce national livestock emissions through the implementation of these dietary interventions.     

Identification of rumen microbial biomarkers linked to methane emission in Holstein dairy cows

Mitigation of greenhouse gas emissions is relevant for reducing the environmental impact of ruminant production. In this study, the rumen microbiome from Holstein cows was characterized through a combination of 16S rRNA gene and shotgun metagenomic sequencing. Methane production (CH₄) and dry matter intake (DMI) were individually measured over 4–6 weeks to calculate the CH₄ yield (CH₄y = CH₄/DMI) per cow. We implemented a combination of clustering, multivariate and mixed model analyses to identify a set of operational taxonomic unit (OTU) jointly associated with CH₄y and the structure of ruminal microbial communities. Three ruminotype clusters (R1, R2 and R3) were identified, and R2 was associated with higher CH₄y. The taxonomic composition on R2 had lower abundance of Succinivibrionaceae and Methanosphaera, and higher abundance of Ruminococcaceae, Christensenellaceae and Lachnospiraceae. Metagenomic data confirmed the lower abundance of Succinivibrionaceae and Methanosphaera in R2 and identified genera (Fibrobacter and unclassified Bacteroidales) not highlighted by metataxonomic analysis. In addition, the functional metagenomic analysis revealed that samples classified in cluster R2 were overrepresented by genes coding for KEGG modules associated with methanogenesis, including a significant relative abundance of the methyl-coenzyme M reductase enzyme. Based on the cluster assignment, we applied a sparse partial least-squares discriminant analysis at the taxonomic and functional levels. In addition, we implemented a sPLS regression model using the phenotypic variation of CH₄y. By combining these two approaches, we identified 86 discriminant bacterial OTUs, notably including families linked to CH₄ emission such as Succinivibrionaceae, Ruminococcaceae, Christensenellaceae, Lachnospiraceae and Rikenellaceae. These selected OTUs explained 24% of the CH₄y phenotypic variance, whereas the host genome contribution was ~14%. In summary, we identified rumen microbial biomarkers associated with the methane production of dairy cows; these biomarkers could be used for targeted methane-reduction selection programmes in the dairy cattle industry provided they are heritable.     

In vitro evaluation,in vivo quantification,and microbial diversity studies of nutritional strategies for reducing enteric methane production

The main objective of the present work was to study nutritive strategies for lessening the CH₄ formation associated to ruminant tropical diets. In vitro gas production technique was used for evaluating the effect of tannin-rich plants, essential oils, and biodiesel co-products on CH₄ formation in three individual studies and a small chamber system to measure CH₄ released by sheep for in vivo studies was developed. Microbial rumen population diversity from in vitro assays was studied using qPCR. In vitro studies with tanniniferous plants, herbal plant essential oils derived from thyme, fennel, ginger, black seed, and Eucalyptus oil (EuO) added to the basal diet and cakes of oleaginous plants (cotton, palm, castor plant, turnip, and lupine), which were included in the basal diet to replace soybean meal, presented significant differences regarding fermentation gas production and CH₄ formation. In vivo assays were performed according to the results of the in vitro assays. Mimosa caesalpineaefolia, when supplemented to a basal diet (Tifton-85 hay Cynodon sp, corn grain, soybean meal, cotton seed meal, and mineral mixture) fed to adult Santa Ines sheep reduced enteric CH₄ emission but the supplementation of the basal diet with EuO did not affect (P > 0.05) methane released. Regarding the microbial studies of rumen population diversity using qPCR with DNA samples collected from the in vitro trials, the results showed shifts in microbial communities of the tannin-rich plants in relation to control plant. This research demonstrated that tannin-rich M. caesepineapholia, essential oil from eucalyptus, and biodiesel co-products either in vitro or in vivo assays showed potential to mitigate CH₄ emission in ruminants. The microbial community study suggested that the reduction in CH₄ production may be attributed to a decrease in fermentable substrate rather than to a direct effect on methanogenesis.     

Structural equation models to disentangle the biological relationship between microbiota and complex traits: Methane production in dairy cattle as a case of study

The advent of metagenomics in animal breeding poses the challenge of statistically modelling the relationship between the microbiome, the host genetics and relevant complex traits. A set of structural equation models (SEMs) of a recursive type within a Markov chain Monte Carlo (MCMC) framework was proposed here to jointly analyse the host–metagenome–phenotype relationship. A non-recursive bivariate model was set as benchmark to compare the recursive model. The relative abundance of rumen microbes (RA), methane concentration (CH₄) and the host genetics was used as a case of study. Data were from 337 Holstein cows from 12 herds in the north and north-west of Spain. Microbial composition from each cow was obtained from whole metagenome sequencing of ruminal content samples using a MinION device from Oxford Nanopore Technologies. Methane concentration was measured with Guardian^® NG infrared gas monitor from Edinburgh Sensors during cow's visits to the milking automated system. A quarterly average from the methane eructation peaks for each cow was computed and used as phenotype for CH₄. Heritability of CH₄ was estimated at 0.12 ± 0.01 in both the recursive and bivariate models. Likewise, heritability estimates for the relative abundance of the taxa overlapped between models and ranged between 0.08 and 0.48. Genetic correlations between the microbial composition and CH₄ ranged from −0.76 to 0.65 in the non-recursive bivariate model and from −0.68 to 0.69 in the recursive model. Regardless of the statistical model used, positive genetic correlations with methane were estimated consistently for the seven genera pertaining to the Ciliophora phylum, as well as for those genera belonging to the Euryarchaeota (Methanobrevibacter sp.), Chytridiomycota (Neocallimastix sp.) and Fibrobacteres (Fibrobacter sp.) phyla. These results suggest that rumen's whole metagenome recursively regulates methane emissions in dairy cows and that both CH₄ and the microbiota compositions are partially controlled by the host genotype.     

Investigation of intra-day variability of gaseous measurements in sheep using portable accumulation chambers

Portable accumulation chambers (PAC) enable short-term spot measurements of gaseous emissions including methane (CH₄), carbon dioxide (CO₂), and oxygen (O₂) consumption from small ruminants. To date the differences in morning and evening gaseous measurements in the PAC have not been investigated. The objectives of this study were to investigate: 1) the optimal measurement time in the PAC, 2) the appropriate method of accounting for the animal’s size when calculating the animal’s gaseous output, and 3) the intra-day variability of gaseous measurements. A total of 12 ewe lambs (c. 10 to 11 months of age) were randomly selected each day from a cohort of 48 animals over nine consecutive days. Methane emissions from the 12 lambs were measured in 12 PAC during two measurement runs daily, AM (8 to 10 h) and PM (14 to 16 h). Animals were removed from Perennial ryegrass silage for at least 1 h prior to measurements in the PAC and animals were assigned randomly to each of the 12 chambers. Methane (ppm) concentration, O₂ and CO₂ percentage were measured at 5 time points (T1 = 0.0 min, T2 = 12.5 min, T3 = 25.0 min, T4 = 37.5 min, and T5 = 50.0 min from entry of the first animal into the first chamber) using an Eagle 2 monitor. The correlation between time points T5-T1 (i.e., 50 min minus 0 min after entry of the animal to the chamber) and T4-T1 was 0.95, 0.92, and 0.77 for CH_4, O₂, and CO_2, respectively (P < 0.01). The correlation between CH₄ and CO₂ output and O₂ consumption, calculated with live-weight and with body volume was 0.99 (P < 0.001). The correlation between the PAC measurement recorded on the same animal in the AM and PM measurement runs was 0.73. Factors associated with CH₄ production included: day and time of measurement, the live-weight of the animal and the hourly relative humidity. Results from this study suggest that the optimal time for measuring an animal’s gaseous output in the PAC is 50 min, that live-weight should be used in the calculation of gaseous output from an animal and that the measurement of an animal’s gaseous emissions in either the AM or PM does not impact on the ranking of animals when gaseous emissions are measured using the feeding and measurement protocol outlined in the present study.     

The influence of extended supplementation of quebracho extract to beef steers consuming a hay diet on digestion,ruminal, and blood parameters

The addition of natural plant secondary compounds to ruminant feed has been extensively studied because of their ability to modify digestive and metabolic functions, resulting in a potential reduction in greenhouse gas emissions, among other benefits. Condensed tannin (CT) supplementation may alter ruminal fermentation and mitigate methane (CH₄) emissions. This study’s objective was to determine the effect of quebracho CT extract [QT; Schinopsis quebracho-colorado (Schltdl.) F.A. Barkley & T. Meyer] within a roughage-based diet on ruminal digestibility and kinetic parameters by using the in situ and in vitro gas production techniques, in addition to blood urea nitrogen (BUN) and ruminal (volatile fatty acid [VFA], NH₃-N, and protozoa count) parameters. Twenty rumen-cannulated steers were randomly assigned to four dietary treatments: QT at 0%, 1%, 2%, and 3% of dry matter (DM; QT0: 0% CT, QT1: 0.70% CT, QT2: 1.41% CT, and QT3: 2.13% CT). The in situ DM digestibility increased linearly (P = 0.048) as QT inclusion increased, whereas in situ neutral detergent fiber digestibility (NDFD) was not altered among treatments (P = 0.980). Neither total VFA concentration nor acetate-to-propionate ratio differed among dietary treatments (P = 0.470 and P = 0.873, respectively). However, QT3 had lower isovalerate and isobutyrate concentrations compared with QT0 (P ≤ 0.025). Ruminal NH₃ and BUN tended to decline (P ≤ 0.075) in a linear fashion as QT inclusion increased, suggesting decreased deamination of feed protein. Ruminal protozoa count was reduced in quadratic fashion (P = 0.005) as QT inclusion increased, where QT1 and QT2 were lower compared with QT0 and QT3. Urinary N excretion tended to reduce in a linear fashion (P = 0.080) as QT increased. There was a treatment (TRT) × Day interaction for in vitro total gas production and fractional rate of gas production (P = 0.013 and P = 0.007, respectively), and in vitro NDFD tended to be greater for QT treatments compared with no QT inclusion (P = 0.077). There was a TRT × Day interaction (P = 0.001) on CH₄ production, with QT3 having less CH₄ production relative to QT0 on day 0 and QT2 on days 7 and 28. Feeding QT up to 3% of the dietary DM in a roughage-based diet did not sacrifice the overall DM digestibility and ruminal parameters over time. Still, it is unclear why QT2 did not follow the same pattern as in vitro gas parameters. Detailed evaluations of amino acid degradation might be required to fully define CT influences on ruminal fermentation parameters and CH₄ production.     

Effects of protein supplementation to steers consuming low-quality forages on greenhouse gas emissions

Providing supplements that enhance the efficiency of feed utilization can reduce methane (CH₄) emissions from ruminants. Protein supplementation is widely used to increase intake and digestion of low-quality forages, yet little is known about its impact on CH₄ emissions. British-cross steers (n = 23; initial body weight [BW] = 344 ± 33.9 kg) were used in a three-period crossover design to evaluate the effect of protein supplementation to beef cattle consuming low-quality forage on ruminal CH₄, metabolic carbon dioxide (CO₂) emissions, forage intake, and ruminal fermentation. Steers individually had ad libitum access to low-quality bluestem hay (4.6% crude protein [CP]) and were provided supplemental protein based on (dry matter basis): cottonseed meal (CSM; 0.29% of BW daily; 391 g/d CP), dried distillers grains with solubles (DDGS; 0.41% of BW daily 563 g/d CP), or none (CON). Urea was added to DDGS to match rumen degradable protein provided by CSM. Ruminal CH₄ and metabolic CO₂ fluxes were obtained 2.4 ± 0.4 times per steer daily using an automated open-circuit gas quantification system (GreenFeed emission monitoring system; C-Lock Inc., Rapid City, SD). Forage intake increased (P < 0.01) with protein supplementation; however, no difference in forage intake (P = 0.14) was observed between CSM and DDGS treatments. Flux of CO₂ (g/d) was greater (P < 0.01) for steers fed CSM and DDGS than for steers fed CON. Steers supplemented with CSM had greater (P < 0.01) CH₄ emissions (211 g/d) than DDGS (197 g/d) both of which were greater (P < 0.01) than CON (175 g/d). Methane emissions as a proportion of gross energy intake (GEI) were lowest (P < 0.01) for DDGS (7.66%), intermediate for CSM (8.46%) steers, and greatest for CON (10.53%). Steers fed DDGS also had the lowest (P < 0.01) ruminal acetate:propionate ratio (3.60), whereas CSM (4.89) was intermediate, and CON (5.64) steers were greatest. This study suggests that the common practice of supplementing protein to cattle consuming low-quality forage decreases greenhouse gas emissions per unit of GEI.     

宏基因组学揭示瘤胃微生物多样性及功能

反刍动物瘤胃内栖息着庞大和复杂的微生物群体,这些微生物与宿主的消化吸收、营养代谢和免疫功能息息相关,宿主及其微生物共同组成了一个"超级生物体"。由于绝大部分瘤胃微生物不可培养,因此以厌氧培养为基础的传统研究方法存在明显的弊端。宏基因组学通过高通量的测序方法,能够全面展示微生物多样性,准确发现新的功能基因。此外,宏基因组学揭示了宿主基因和微生物组之间的互作关系。随着组学技术的不断发展,宏基因组学在瘤胃微生物组研究方面具有广阔的应用前景。     

Ranking cows’ methane emissions under commercial conditions with sniffers versus respiration chambers

This study assessed the ranking of dairy cows using individual-level correlations for methane (CH₄) emission on-farm using sniffers and in respiration chambers. In total 20 lactating dairy cows, ten Holstein and ten Jerseys were recorded using sniffers installed in milking robots for three weeks of lactation and subsequently in respiration chambers (RC) where they were each recorded on three occasions within the RC. Bivariate linear mixed models were used to determine the individual-level correlations (r_I) between sniffer and RC phenotypes as proxies for genetic correlations. Despite differences in feeding and management, the predicted CH₄ production from sniffers correlated highly with RC CH₄ production r_I?=?0.77?±?0.18 and CH₄ breath concentration correlated nearly as well with RC CH₄ production r_I?=?0.75?±?0.20. These correlations between sniffers on-farm and RC demonstrate the potential of sniffers measurements as large-scale indicator traits for CH₄ emissions in dairy cattle.     

Expected consequences of including methane footprint into the breeding goals in beef cattle. A Spanish Blonde d'Aquitaine population as a case of study

This study evaluates two potential scenarios for including methane (CH₄) emissions in the breeding objectives of beef cattle, using the Spanish population of Blonde d′Aquitaine as a case of study. First, CH₄ emissions were included as a cost using a shadow carbon price of 1.22€/CH₄ kg (0.044€/CO₂ kg) (carbon tax scenario). In the other scenario, a CH₄ quota was applied, optimizing emissions per unit of product. The current production system was used as benchmark scenario (Scenario 1). The economic value of CH₄ was calculated under all scenarios using a bioeconomic model that translated the production system into a mathematical function. Then, CH₄ emissions were included with proper relative weight in the selection index under each scenario. The economic value of CH₄ production from cows was ?0.54€/year and ?0.16€/year in a carbon tax and in a CH₄ quota scenario, respectively. Economic values for CH₄ production from fattening calves were ?1.22€/year and ?0.34€/year in a carbon tax and a quota scenario, respectively. The relative weights of total CH₄ traits in the indices were 4.9% and 1.8% in a carbon tax and quota scenario. The carbon tax scenario led to smaller cows (?7.59 kg of mature weight) and a decrease in carcass weight gain of calves (?4.78 g/day) involving a reduction in emissions in comparison with Scenario 1 (?0.76 CH₄ kg/slaughtered calf/year). However, it also led to a lower expected gain in profit per unit of product (?7.86 €/slaughtered calf/year). A carbon quota scenario would select slightly smaller cows (?0.48 kg) with similar responses in maternal abilities (age at first calving, calving interval, maternal weaning weight, and calving ease) and growth, and lower emissions (?0.22 CH₄ kg/slaughtered calf/year) regarding the benchmark scenario. Profit per cow would increase by +1.52€/slaughtered calf/year although this scenario implies a reduction in the number of cows per herd. In a carbon tax scenario, higher reduction in emissions implied a reduction of profitability per animal.     

Combined effects of 3-nitrooxypropanol and canola oil supplementation on methane emissions,rumen fermentation and biohydrogenation,and total tract digestibility in beef cattle

The individual and combined effects of 3-nitrooxypropanol (3-NOP) and canola oil (OIL) supplementation on enteric methane (CH₄) and hydrogen (H₂) emissions, rumen fermentation and biohydrogenation, and total tract nutrient digestibility were investigated in beef cattle. Eight beef heifers (mean body weight ± SD, 732 ± 43 kg) with ruminal fistulas were used in a replicated 4 × 4 Latin square with a 2 (with and without 3-NOP) × 2 (with and without OIL) arrangement of treatments and 28-d periods (13 d adaption and 15 d measurements). The four treatments were: control (no 3-NOP, no OIL), 3-NOP (200 mg/kg dry matter [DM]), OIL (50 g/kg DM), and 3-NOP (200 mg/kg DM) plus OIL (50 g/kg DM). Animals were fed restrictively (7.6 kg DM/d) a basal diet of 900 g/kg DM barley silage and 100 g/kg DM supplement. 3-NOP and OIL decreased (P < 0.01) CH₄ yield (g/kg DM intake) by 31.6% and 27.4%, respectively, with no 3-NOP × OIL interaction (P = 0.85). Feeding 3-NOP plus OIL decreased CH₄ yield by 51% compared with control. There was a 3-NOP × OIL interaction (P = 0.02) for H₂ yield (g/kg DM intake); the increase in H₂ yield (P < 0.01) due to 3-NOP was less when it was combined with OIL. There were 3-NOP × OIL interactions for molar percentages of acetate and propionate (P < 0.01); individually, 3-NOP and OIL decreased acetate and increased propionate percentages with no further effect when supplemented together. 3-NOP slightly increased crude protein (P = 0.02) and starch (P = 0.01) digestibilities, while OIL decreased the digestibilities of DM (P < 0.01) and neutral detergent fiber (P < 0.01) with no interactions (P = 0.15 and 0.10, respectively). 3-NOP and OIL increased (P = 0.04 and P < 0.01, respectively) saturated fatty acid concentration in rumen fluid, with no interaction effect. Interactions for ruminal trans-monounsaturated fatty acids (t-MUFA) concentration and percentage were observed (P = 0.02 and P < 0.01); 3-NOP had no effect on t-MUFA concentration and percentage, while OIL increased the concentration (P < 0.01) and percentage (P < 0.01) of t-MUFA but to a lesser extent when combined with 3-NOP. In conclusion, the CH₄-mitigating effects of 3-NOP and OIL were independent and incremental. Supplementing ruminant diets with a combination of 3-NOP and OIL may help mitigate CH₄ emissions, but the decrease in total tract digestibility due to OIL may decrease animal performance and needs further investigation.     

Effects of feeding lubabegron on gas emissions,growth performance,and carcass characteristics of beef cattle housed in small-pen environmentally monitored enclosures during the last 3 mo of the finishing period

The development of technologies that promote environmental stewardship while maintaining or improving the efficiency of food animal production is essential to the sustainability of producing a food supply to meet the demands of a growing population. As such, Elanco (Greenfield, IN) pursued an environmental indication for a selective β-modulator (lubabegron; LUB). LUB was recently approved by the United States Food and Drug Administration (FDA) to be fed to feedlot cattle during the last 14 to 91 d of the feeding period for reductions in gas emissions/kg of unshrunk final BW and HCW. A 4 × 2 factorial arrangement of treatments was used with the factors of dose (0.0, 1.38, 5.5, or 22.0 mg·kg⁻¹ DM basis) and sex (steers or heifers). Three 91-d cycles were conducted (112 cattle/cycle) with each dose × sex combination being represented by a single cattle pen enclosure (CPE; 14 cattle/CPE) resulting in a total of 168 steers and 168 heifers (n = 6 replicates/dose). There were no interactions observed between dose and sex for any variable measured in the study (P ≥ 0.063). Five gases were evaluated for all pens based on CPE concentrations relative to ambient air: NH₃, CH₄, N₂O, H₂S, and CO₂. Cumulative NH₃ gas emissions were reduced by feeding cattle 5.5 and 22.0 mg·kg⁻¹ LUB (P ≤ 0.023) and tended (P = 0.076) to be lower for the cattle fed 1.38 mg·kg⁻¹ LUB compared with the negative controls (CON). The cumulative NH₃ gas emission reductions of 960 to 1032 g, coupled with HCW increases (P ≤ 0.019) of 15 to 16 kg for all LUB doses vs. CON, led to reductions in NH₃ gas emissions/kg HCW for all three LUB treatments (P ≤ 0.004). Similar to HCW, reductions in NH₃ gas emissions/kg of unshrunk final BW were observed for all LUB doses (P ≤ 0.009) and were attributable to both decreases in NH₃ gas emissions and numerical increases in BW. Dose had no effect on cumulative emissions or emissions standardized by BW or HCW for the other four gases (P ≥ 0.268). LUB is a novel tool to reduce emissions of NH₃ gas per kilogram of unshrunk live BW and hot carcass weight.     

Dose–response effect of a pine bark extract on in vitro ruminal ammonia and methane formation kinetics

ABSTRACT

This study assessed the potential of a pine bark extract (PBE) to decrease methane (CH₄) and ammonia nitrogen (NH₃-N) production in vitro. Dietary substrates, mixed hay, soybean meal and corn grain, were supplemented 0, 2, 4 and 6% of PBE and incubated in an in vitro batch culture for 24-h. Incubations were run three times. Total gas production (GP) was determined at 6, 12 and 24-h and gas samples were analysed for CH₄. Samples were collected for volatile fatty acids (VFA) and NH₃-N analysis. Treatments were compared by polynomial contrasts for PBE concentration. Increasing PBE caused linear decreases in NH₃-N, microbial biomass production and digestibility, whereas the degradation rate was quadratically reduced. Total VFA were decreased but total GP and CH₄ production and kinetics were unaffected. The inclusion of 2% PBE in ruminant feed has the capability to reduce NH₃-N concentration by 50%, without affecting diet digestibility or CH₄ production.     

Evaluation of a single‐flow continuous culture fermenter system for determination of ruminal fermentation and enteric methane production

A 4‐unit, single‐flow continuous culture fermenter system was developed to assess in vitro nutrient digestibility, volatile fatty acid (VFA) concentration and daily enteric methane (CH₄) production of ruminant diets. The objective was to develop a closed‐vessel system that maintained protozoal populations and provided accurate predictions of total CH₄ production. A diet of 50% orchardgrass (Dactylis glomerata L.) and 50% alfalfa (Medicago sativa L.) was fed during 4, 10‐day periods (7‐day adaptation and 3‐day collection). Fermenters were fed 82 g of dry matter (DM)/day in four equal feedings. pH and temperature were taken every 2 min, and CH₄ concentration was measured every 10 min. Samples for DM and protozoal counts were taken daily, and daily effluent samples were collected for determination of DM, VFA and NH₃‐N concentrations. There was no effect (p > 0.17) of adaptation versus collection days on vessel and effluent DM, temperature or pH. Initial protozoal counts decreased (p < 0.01), but recovered to initial counts by the collection period. Total VFA, acetate, propionate and isobutyrate concentrations did not differ (p ≥ 0.13) among periods or days of the collection period. There was no difference (p ≥ 0.37) among days or periods in total daily CH₄ production and CH₄ production per g of OM, NDF, digestible OM or digestible NDF fed. Data collected throughout 4 experimental periods demonstrated that the system was able to reach a steady state in fermentation well within the 7‐day adaptation period and even typically variable data (i.e., CH₄ production) were stable within and across periods. While further research is needed to determine the relationship between this system and in vivo data, this continuous culture fermenter system provides a valid comparison of in vitro ruminal fermentation and enteric CH₄ production of ruminant diets that can then be further validated with in vivo studies.     

Methane emissions and δ13C composition from beef steers consuming increasing proportions of sericea lespedeza hay on bermudagrass hay diets

An experiment was conducted to evaluate the effects of different proportions of ‘Au Grazer’ sericea lespedeza [SL, Lespedeza cuneata (Dum. Cours.) G. Don], a legume rich in condensed tannins (CT), on nutrient intake and digestibility, and to estimate methane (CH₄) emissions and ¹³C isotopic composition (δ¹³C_CH4) from beef steers consuming a forage-based diet. Twenty-five Angus-crossbred steers were distributed in a randomized complete block design (344 ± 48 kg initial BW), and randomly assigned to one of five treatments: 0SL, 25SL, 50SL, 75SL, and 100SL, diets containing 0%, 25%, 50%, 75%, and 100% of SL hay, respectively, mixed with ‘Tifton-85’ bermudagrass hay (Cynodon spp.). The study was carried out for two experimental periods of 21-d each. The statistical model included the fixed effect of treatment and random effects of block, experimental period, and their interaction. Apparent total tract digestibility of crude protein, neutral detergent fiber, and acid detergent fiber was linearly decreased (P < 0.001) by the inclusion of SL. No effects were observed for total CH₄ emissions per day, nor for CH₄ relative to organic matter intake or digestible organic matter with the inclusion of SL. However, emission of CH₄ in relation to intake of CT was affected by treatment (P < 0.001). A linear (P < 0.001) decrease and a quadratic effect (P < 0.001) were observed for δ¹³C of diets and gas, respectively, in which diets and enteric CH₄ with greater inclusion of SL were more depleted in ¹³C. Moreover, the difference in δ¹³C between diets and gas (Δδ¹³C) had a linear decrease (P = 0.001) with the inclusion of SL. The model developed to predict the C₃ proportions in the enteric CH₄ fitted to predicted values (P < 0.0001). Therefore, greater proportions of SL resulted in lesser CH₄ emission when CT intake was considered and the isotopic composition from enteric CH₄ was able to predict the contribution of SL in the emissions.     

Development of enteric methane emission factors for Holstein-Friesian and Norwegian first lactation cows

Abstract

The objectives were to accurately quantify enteric methane (CH₄) emissions for first lactation dairy cows and to use these data to develop CH₄ prediction equations. Calorimeter measurements and production data were used to calculate CH₄ emissions for Holstein-Friesian (HF, n?=?32) and Norwegian (n?=?32) first lactation cows during a 305-d lactation period. Methane outputs were similar between HF and Norwegian (123 vs. 126?kg/305 d) when offered high-concentrate diets, but HF produced more CH₄ (P?<?0.05) than Norwegian (105 vs. 98?kg/305 d) when given low-concentrate diets. The HF offered high-concentrate diets had a lower (P?<?.05) CH₄ emission per energy-corrected milk yield (16.3?g/kg) than the other three treatments (19.7–20.4?g/kg). These data were then used to develop CH₄ prediction equations, which provide an alternative approach to estimate enteric CH₄ emissions for HF and Norwegian first lactation dairy cows.     

Historic, pre-European settlement, and present-day contribution of wild ruminants to enteric methane emissions in the United States

The objectives of this analysis were to estimate historic (pre-European settlement) enteric CH(4) emissions from wild ruminants in the contiguous United States and compare these with present-day CH(4) emissions from farmed ruminants. The analysis included bison, elk (wapiti), and deer (white-tailed and mule). Wild ruminants such as moose, antelope (pronghorn), caribou, and mountain sheep and goat were not included in the analysis because their natural range is mostly outside the contiguous United States or because they have relatively small population sizes. Data for presettlement and present-day population sizes, animal BW, feed intake, and CH(4) emission factors were adopted from various sources. Present-day CH(4) emissions from livestock were from recent United States Environmental Protection Agency estimates. The most important factor determining CH(4) emissions from wild ruminants in the presettlement period was the size of the bison population. Overall, enteric CH(4) emissions from bison, elk, and deer in the presettlement period were about 86% (assuming bison population size of 50 million) of the current CH(4) emissions from farmed ruminants in the United States. Present-day CH(4) emissions from wild ruminants (bison, elk, and deer) were estimated at 0.28 Tg/yr, or 4.3% of the emissions from domestic ruminants. Due to its population size (estimated at 25 million), the white-tailed deer is the most significant present-day wild ruminant contributor to enteric CH(4) emissions in the contiguous United States.