牛、羊布鲁氏菌病试管凝集试验与微量凝集试验检测方法的比较

血清学检测方法因其快速、安全、高效等特点被广泛应用于布鲁氏菌病的检测和诊断。本文通过对保存的226份牛、羊血清样品同时使用试管凝集法与微量凝集试验进行检测并比较分析其在诊断中的意义。结果显示,微量法与试管法两者的符合率为97.79%,两种检测方法无统计学差异。但微量法的阳性检出率要高于试管法,同时由于微量法具有操作简便、快捷及结果判定更为直观、简单等优点,因此,微量凝集试验更适宜在我国基层布鲁氏菌病大量样本监测、诊断中推广应用。     

The vaginal mucus agglutination test in the diagnosis of bovine brucellosis

Of 1140 vaginal mucus agglutination tests (VMAT) on specimens obtained in 1971-72 from 663 dairy cows in seven herds infected with brucellosis, 97 were positive. When the VMAT was positive one or more serological tests were also positive. Of the 97 corresponding serum agglutination tests 80 sera had titres of more than 533 international units. Only 69.8 per cent of VMAT from serologically positive cows were positive. No evidence was found of non-specific agglutinins in vaginal mucus and positive VMAT reactions appeared to be specific for field infection. Three cows showed evidence of local agglutinins in the vagina. Hence herd testing by VMAT has no advantage over tests of blood serum but the test could be an aid in establishing whether individual cattle are infected.     

应用斑点酶联免疫试验快速检测绵羊布鲁氏菌病

Dot- ELISA首先为 Hankes和 Harbrink等建立。Pappas等正式称其为 Dot- ELISA。鉴于本方法无需复杂设备 ,目前已作为一种免疫酶技术在医学领域广泛应用。将本技术应用于绵羊布鲁氏菌( Brucella ovis)病的诊断 ,目前尚未见资料报道。本文旨在建立本学科的试验方法 ,并证明其方法的可靠性和可行性。1　材料与方法1 .1　载体　混合纤维素酶微孔滤膜 (上海产 ,批号870 82 9)。1 .2　试剂　 Br.ovis菌体抗原、热酚抗原、超声提取抗原、碱提取抗原均为新疆流研所刘志文制备。酶标记兔抗羊 Ig G由上海生物制品研究所提供。1 .3　制膜　取前…     

Evaluation of a microplate agglutination test (MAT) for serological diagnosis of canine brucellosis

A microplate agglutination test (MAT) was compared with the tube agglutinin test (TAT), a standard test for the diagnosis of Brucella canis, in terms of the sensitivity and specificity. The results showed that MAT was more sensitive, simpler to perform and easier to read the results than TAT. On top of that the MAT allows us to handle a larger number of samples at once. Using this method we conducted sero-surveillance of the prevalence of B. canis in dogs kept in an Animal Shelter located in Kanagawa Prefecture. Twelve of 485 (2.5%) showed seropositive against B. canis. These results indicate that B. canis infection in dogs is still occurring in Japan.     

外购动物布鲁氏菌病流行情况

布鲁氏菌病 (以下简称布病 )是由布鲁氏菌引起的人、畜共患的慢性传染病。一旦发生 ,将给畜牧业和人民身体健康带来严重危害。从 1 986年开始 ,伊通县一直坚持“检疫、免疫、淘汰病畜”等综合性防制措施 ,基本达到了稳定控制区标准。但是 ,近几年来 ,发现畜间布病有所抬头。从     

A comparison of counter-immunoelectrophoresis with the rose bengal and the serum tube agglutination tests in the diagnosis of brucellosis in sheep

The counter-immunoelectrophoresis test (CIEPT) was compared with the rose bengal plate test (RBPT) and the serum agglutination test (SAT) in the diagnosis of brucellosis in 241 sheep. The total number of animals positive by one or more of the tests used was 106. Sixty animals were positive by all tests, while 87, 79 and 80 were positive by CIEPT, RBPT and SAT respectively. Based on the assumption that animals with an SAT titre of 40 I.U./ml or above were true positives, the CIEPT had a sensitivity of 82.5% and a specificity of 78.3% compared to 96.5% and 87% respectively, demonstrated by the RBPT. CIEPT might be used in flocks without clinical evidence of ram epididymitis if a test to supplement RBPT were required; but it would only partially replace SAT. Sera positive in RBPT but negative in CIEPT would have to be tested by SAT. With this combination, only about 22% of sera positive in RBPT would need to be further tested by SAT.     

Comparison of agar gel immunodiffusion test, rapid slide agglutination test, microbiological culture and PCR for the diagnosis of canine brucellosis

The performance of the rapid slide agglutination test, with and without 2-mercaptoethanol (RSAT and 2ME-RSAT) and agar gel immunodiffusion test (AGID) was evaluated for the diagnosis of brucellosis in naturally infected dogs. The microbiological culture, PCR and clinical parameters were used as reference. A total of 167 dogs were clinically examined and tested by blood culture, culture of semen/vaginal swab and PCR in blood and semen/vaginal swab. According to the results observed the 167 dogs were divided into three groups: Brucella canis infected dogs (Group 1), B. canis non-infected dogs (Group 2) and dogs with suspected brucellosis (Group 3). The dogs were then tested by RSAT, 2ME-RSAT and AGID. Groups 1 and 2 were used to calculate the diagnostic sensitivity and specificity of the serological tests and the results observed in Group 3 were also discussed. The diagnostic sensitivity of RSAT, 2ME-RSAT and AGID was respectively 70.58%, 31.76%, and 52.94%. The diagnostic specificity of RSAT, 2ME-RSAT and AGID was respectively 83.34%, 100%, and 100%. In dogs with suspected brucellosis 15% were RSAT positive, none was 2ME-RSAT positive and 5% were AGID positive. Although the serological tests are the most commonly used methods for brucellosis diagnosis, a significant proportion of false-negative results were observed highlighting the importance of the direct methods of diagnosis, like blood culture and PCR to improve the diagnosis of canine brucellosis.