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建立了测定头孢噻呋钠含量的高效液相色谱法。采用C18色谱柱(4.6mm×250mm,5μm),以含0.8%四正丁基溴化铵的乙腈溶液-0.1mol/L柠檬酸钠溶液(用20%柠檬酸调节pH至5.0)-磷酸盐溶液(0.1mol/L磷酸二氢钾,0.02mol/L磷酸氢二钾,用10mol/L氢氧化钾调节pH至7.0)-水(400:4:48:520)为流动相,流速1.0mL/min,检测波长254nm,进样量20μL,柱温30℃。头孢噻呋钠浓度在0.0496~0.1984mg/mL时,其峰面积与浓度的线性关系良好(r=1.000);平均回收率为100.0%,平均相对标准偏差RSD为0.17%;检测限为0.0025μg/mL。本方法操作简便,分析快速,结果准确,适用于头孢噻呋钠原料药的含量测定。 相似文献
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高效液相色谱法测定盐酸林可霉素注射液的含量 总被引:1,自引:0,他引:1
建立盐酸林可霉素注射液含量测定的高效液相色谱法。色谱柱:C_(18)色谱柱(4.6 mm×250 mm,5μm),流动相:0.05 mol/L硼砂溶液(用85%磷酸溶液调节pH值至5.0)∶甲醇∶乙腈=67∶33∶2(V/V),流速:1 mL/min,检测波长:214 nm,进样量:10μL,柱温:30℃。盐酸林可霉素浓度在0.1-2 mg/m L范围内峰面积与浓度的线性关系良好(R~2=0.999 9)。此法简便、快捷、灵敏度高、重现性好。 相似文献
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HPLC法测定盐酸小檗碱预混剂的含量 总被引:3,自引:0,他引:3
采用HPLC法测定盐酸小檗碱预混剂中盐酸小檗碱含量。色谱柱为Agilent ZORBAXSB-C18柱(3.9mm×150mm,4μm),流动相为0.02mol/L磷酸二氢钾溶液(用磷酸调pH为2.5)-乙腈(70∶30,V/V),检测波长346nm,流速1.0mL/min,柱温为30℃,保留时间约6.2min。盐酸小檗碱浓度在5~80μg/mL范围内,峰面积与浓度呈良好的线性关系(r2=0.9999)。平均回收率99.5%,RSD为0.4%。该方法适用于检测盐酸小檗碱预混剂。 相似文献
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目的:建立酒石酸艾芬地尔注射液中的酒石酸艾芬地尔的含量测定方法.方法:采用C18色谱柱(4.6 mmX200 mm,5 μm),流动相为0.02mol/L磷酸二氢钾的0.1%三乙胺溶液(用磷酸调节pH至4.0)-甲醇(45:55),流速:1.0m1·min-t检测波长:275nm.结果酒石酸艾芬地尔的线性方程为A=133093+1714033 r=0.9999;酒石酸艾芬地尔的回收率为99.86%,RSD为0.61%.结论HPLC法准确、可靠、灵敏度高、重复性好,可用于酒石酸艾芬地尔注射液的质量控制. 相似文献
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建立了测定醋酸氯己定含量的高效液相色谱法。以C18(4.6 mm×150 mm,5μm)为色谱柱,0.02 mol/L磷酸二氢钾溶液(pH2.6)-乙腈(70∶30)为流动相,流速1 mL/min,检测波长254 nm。结果显示,醋酸氯己定在0.5~198μg/mL浓度范围内,浓度对峰面积的线性关系良好(r=0.999 98),平均回收率为96.40%(n=9)。实验表明,该方法灵敏、准确,可以较好地控制醋酸氯己定子宫灌注剂的质量。 相似文献
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建立并验证了喹乙醇预混剂中喹乙醇含量的高效液相色谱测定法。试验采用十八烷基硅烷键合硅胶色谱柱(250 mm×4.6 mm,5μm),以0.02 mol/L磷酸二氢钾溶液(用三乙胺调节pH值为6.0)-乙腈(92∶8,V∶V)为流动相,流速为1.0 mL/min,柱温为30℃,检测波长为262 nm。试验结果表明喹乙醇在5~100μg/mL范围内线性良好,R~2=1.0000,平均回收率100.2%(n=6),RSD为0.2%。建立的方法简便、准确、可靠,可更好地控制喹乙醇预混剂的质量。 相似文献
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魏静 《四川畜牧兽医学院学报》2009,(4):28-32
在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。 相似文献
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以国际标准强毒R株人工感染非免疫产蛋鸡,定时扑杀,分别从鼻窦、眶下孔、气管、肺、气囊、卵巢和输卵管分离MG,并收集感染鸡所产蛋分离MG。结果表明,人工感染48小时后上、下呼吸道及肺已被全面感染,96小时气囊已被感染,120小时输卵管已能分离到MG,卵巢始终分离不到MG。人工感染鸡自144小时便能在其所产蛋中分离出MG。药物治疗能在72小时内消除感染,油乳剂苗则需24天后逐渐降低蛋内MG分离率,药物卵内注射、种蛋药浴、高温处理均能杀死卵内MG,但以研制的种蛋浸泡剂药浴效果为最好。 相似文献
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本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。 相似文献
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REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air. 相似文献
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