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对EDTA和KMnO4滴定法测定饲料中钙进行了比较研究。结果表明,两种方法的准确度和精密度都非常好。变异系数均低于4.0%,RSD也在规定的范围以内,EDTA法回收率为98.53%~100%。高锰酸钾法回收率为97.33%~99.53%。因此,EDTA法可用于教学试验中钙的测定和生产品控过程中饲料钙的测定。 相似文献
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微分脉冲催化极谱法测定乳牛全血硒 总被引:1,自引:0,他引:1
采用美国273A型电化学分析系统,根据SeSO32--KIO3氧化还原反应原理,用微分脉冲催化极谱法测定了奶牛全血痕量硒。此法最低检出极限0.1μg/L,线性范围0.5~5.0μg/L,回收率92.60%~103.00%。硒标准溶液和饲料重复测定值的变异系数分别为1.5%和2.2%。奶牛全血硒的测定值符合饲料梯度添加试验,其精密度和准确度均高。 相似文献
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黄酮类成分因其具有多样生物活性成为目前天然产物研究的热点。本实验探讨了聚酰胺-UV法作为蜂花粉总黄酮测定方法的可行性。结果表明以芦丁为标准品,以360nm为检测波长,用聚酰胺-UV法检测蜂花粉总黄酮在0.005-0.025mg/mL范围内,浓度与吸光度呈良好的线性关系,相关系数R2为0.9998,回收率、稳定性、精密度和重现性均理想,其RSD分别为2.48%(n=3),2,12%(n=3),0.49%(n=3)、0.88%(n=5)。该法检测油菜蜂花粉中总黄酮含量为1.54%。 相似文献
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采用两种不同的色谱条件测定吡喹酮注射剂中吡喹酮的含量,色谱柱为迪马DiamonsilC18(4.6mm×250mm,5μm)柱,流动相为甲醇-水及乙腈-水,检测波长为263nm及210nm,流速1mL/min。结果显示,在两个不同的检测范围内,线性关系均良好(分别为r=0.9998和r=0.9996),吡喹酮的平均回收率为100.38%和99.90%,RSD=1.13%和1.1%(n=9)。两种方法简便易行,精密度、回收率、重现性均符合要求。结果表明,两种方法均可用于吡喹酮注射剂的含量测定。 相似文献
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本文建立了生鲜牛乳中三聚氰胺的高效液相色谱测定方法。该法采用ODS—C8分离柱,紫外检测器进行样品检测(波长240nm)。该方法简单、快速、重现性好,平均回收率大于85%,精密度(RSD)小于3.0%,线性范围为1-100mg/L。 相似文献
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动物组织中磺胺二甲嘧啶残留检测ELISA试剂盒的研制 总被引:25,自引:4,他引:21
在建立竞争ELISA方法的基础上,首次研制出检测磺胺二甲嘧啶的单克隆抗体快速检测试剂盒,并对其检测限、精密度、检测范围以及鸡肌肉组织中的添加回收实验做了详细研究。本试剂盒的检测限为1.0ng/m1,检测范围为1.0-81.0ng/m1,批内变异系数<8.9%,批间变异系数<9.5%,在10、60和200ng/m1水平鸡肌肉组织中添加,回收率为64.5%-85.5%,变异系数为6.0%-18.6%。与同类相关德国产试剂盒相比较,阳性符合率为100%。 相似文献
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用MycoSepTM净化柱和高效液相色谱法对谷物中黄曲霉毒素B1、G1、B2、G2的测定 总被引:4,自引:0,他引:4
用一种快速方便的MycoSep^TM净化柱和高效液相色谱法对谷物中黄曲霉毒素B1、G1、B2、G2进行测定。样品经乙腈-水(体积比84:16)提取,提取液通过MycoSep^TM净化柱净化、浓缩,三氟乙酸(TFA)柱前衍生,C18色谱柱分离,荧光检测器检测,外标法定量。对添加两个浓度黄曲霉毒素的玉米样品进行加标回收实验,4种毒素的回收率均在90.44%-101.23%之间,B1、G1、B2、G2的最低检测限分别为2.0、1.0、0.5、0.5ng/kg。实验结果表明,此法不仅定量准确,精密度高而且操作极为方便。 相似文献
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Meschan EM Peham C Schobesberger H Licka TF 《Veterinary journal (London, England : 1997)》2007,173(3):578-584
As there is no statistical evidence that saddle fit influences the load exerted on a horse's back, this study was performed to assess the hypothesis that the width of the tree significantly alters the pressure distribution on the back beneath the saddle. Nineteen sound horses were ridden at walk and trot on a treadmill with three saddles differing only in tree width. Kinetic data were recorded by a sensor mat. A minimum of 14 motion cycles were used in each trial. The saddles were classified into four groups depending on fit. For each horse, the saddle with the lowest overall force (LOF) was determined. Saddles were classified as "too-narrow" if they were one size (2 cm) narrower than the LOF saddle, and "too-wide" if they were one size (2 cm) wider than the LOF saddle. Saddles two sizes wider than LOF saddles were classified as "very-wide". In the group of narrow saddles, the pressure in the caudal third (walk 0.63 N/cm(2)+/-0.10; trot 1.08 N/cm(2)+/-0.26) was significantly higher compared to the LOF saddles (walk 0.50 N/cm(2)+/-0.09; trot 0.86 N/cm(2)+/-0.28). In the middle transversal third, the pressure of the wide saddles (walk 0.73 N/cm(2)+/-0.06; trot 1.52 N/cm(2)+/-0.19) and very-wide saddles (walk 0.77 N/cm(2)+/-0.06; trot 1.57 N/cm(2)+/-0.19) was significantly higher compared to LOF saddles (walk 0.65 N/cm(2)+/-0.10/ 0.63 N/cm(2)+/-0.11; trot 1.33 N/cm(2)+/-0.22/1.27 N/cm(2)+/-0.20). This study demonstrates that the load under poorly fitting saddles is distributed over a smaller area than under properly fitting saddles, leading to potentially harmful pressures peaks. 相似文献
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M. Masko M. Domino K. Skierbiszewska Ł. Zdrojkowski T. Jasinski Z. Gajewski 《Equine Veterinary Education》2020,32(12):654-663
Complications in late gestation are challenging problems for the equine clinician and are causes of considerable distress to owners. Health problems in a pregnant mare in each stage of gestation can adversely affect the pregnancy. It is important to obtain a thorough history and perform a detailed and systematic examination of the pregnant mare so that the mare may be classified as normal or high-risk. However, even after a thorough examination, practitioners may face problems with the interpretation and assessment of risk. Normal mares should foal in the stable or pasture. Mares classified as having a high-risk pregnancy need to be monitored precisely for signs of oncoming foaling with attendance of trained personnel when labour begins. A valuable method for monitoring of the high-risk mare is assessing fetoplacental well-being. Fetoplacental well-being is most accurately assessed with ultrasonography as well as several available hormone profiles. We are proposing our scoring system of the mare's health, which may be useful in the assessment of risk during pregnancy and help clinicians to decide whether sending the mare to the clinic is reasonable or unnecessary. 相似文献
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Webbon P 《Equine veterinary journal》2012,44(1):8-12
The understanding and application of genetics have grown extremely quickly since it has become possible to sequence the whole genome of an organism. The human genome sequence was completed in 2001 and that of the horse in 2007. The significance of this is that it makes it more feasible to explain how both genetically simple and complex traits are transmitted from one generation to the next and, therefore, to make informed breeding decisions, modify how horses are managed and trained to minimise the risk of disease and injury, and improve methods of prevention, diagnosis and treatment of many conditions. The science of genetics/genomics will continue to grow internationally, limited only by the funds available. The application of the science to man, horses and other species raises very complex moral and commercial issues. Thoroughbred breeders are perceived by some as resistant to change, but their apparent intransigence is often based on a genuine concern for the integrity of the breed. By taking control of the application of the advances in genetics, the Thoroughbred industry potentially has the opportunity to improve both the health and performance of Thoroughbreds. If, however, the science is applied in an uncoordinated manner, driven by commercial interests with no underlying concern for the horses themselves, there is a very real risk that breeders, the Thoroughbred breed and individual horses will all suffer as a consequence. 相似文献
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为了找出支配鸡胆囊交感节后神经元的分布规律,选用体重1.5 kg~2.5 kg的成年母鸡6只,将CT-HRP溶液注入胆囊壁,动物存活3 d~4 d后,经左心室灌流固定,取内脏神经节、肾上腺神经节以及双侧胸、腰和荐段交感干神经节,制成50 μm的连续冰冻切片,TMB法呈色反应,置明视野显微镜下观片统计.结果发现,支配鸡胆囊的交感传出神经元胞体位于内脏神经节(占41.1%)、肾上腺神经节(占40.5%)和T2~T7交感干神经节(占18.4%),在交感干神经节中标记细胞的峰值位于T5、T6交感干神经节.所有的标记细胞以位于右侧的占优势. 相似文献
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犊牛腹泻与肠道菌群的变化 总被引:1,自引:0,他引:1
本文检测腹泻犊牛的直肠内8种主要正常菌群的变化,并与健康犊牛进行对比。结果发现,犊牛腹泻时,大肠杆菌、双岐杆菌和乳杆菌变化较大,而其它菌群变化较小。腹泻或将腹泻时,大肠杆菌数增加,双岐杆菌和乳杆菌数下降。腹泻康复或即将康复时,大肠杆菌减少,双岐杆菌、乳杆菌数增加。 相似文献
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试验选择陕北白绒山羊繁育中心羊场的成年健康母羊(怀孕前期)16只,育成母羊13只,育成公羊13只,空腹称重。测定每只羊1d的采食量,计算其摄入的营养成分含量,并与原苏联绒用山羊饲养标准比较,进行饲养水平评价。结果显示,与饲养标准比较,陕北白绒山羊繁育中心羊场的育成母羊代谢能高2.68MJ,粗蛋白低59.29g,钙低3.00g,磷低1.52g;育成公羊代谢能高3.47MJ,粗蛋白低81.92g,钙低4.42g,磷低2.62g;成年母羊代谢能高2.51MJ,粗蛋白低91.33g,钙低3.09g,磷低1.93g。结合羊的体况综合评价饲养水平基本合理,但尚需要按饲养标准增加粗蛋白、钙、磷等营养物质的供给量。 相似文献