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1.
Suepaul SM Carrington CV Campbell M Borde G Adesiyun AA 《Tropical animal health and production》2011,43(2):367-375
A study was conducted to determine the seroprevalence of leptospirosis and infecting serovars across livestock (cattle, sheep,
goats, and pigs) in Trinidad using the microscopic agglutination test with an international panel of 23 serovars. Of a total
of 590 cattle tested, 21.5% were seropositive with agglutinations to 13 of the 23 antigens used in the panel. Icterohaemorrhagiae
(9.3%), Sejroe (4.1%), Ballum (4.1%), and Autumnalis (1.9%) were the predominant serogroups detected in the cattle sampled
(n = 590). Of 222 sheep tested, 5.0% were seropositive with agglutinations to five serovars belonging to two serogroups. These
serogroups were Autumnalis at 2.7%, and Icterohaemorrhagiae at 2.3% of all sheep tested (n = 222). Of a total of 180 goats tested, 3.3% were seropositive, all agglutinating to the Icterohaemorrhagiae serogroup, 1.7%
to serovar Copenhageni, 1.1% to serovar Mankarso, and 0.6% to serovar Icterohaemorrhagiae. Among pigs (n = 200), 5.0% were seropositive for five serovars belonging to three serogroups. These serogroups were Icterohaemorrhagiae
at 2.5%, Australis at 2%, and Ballum at 0.5%. Overall, age and sex of animals were not significantly associated with leptospirosis
with the exception of cattle where age was a significant factor for seropositivity. It was concluded that for livestock, leptospirosis
may be an important zoonotic and economic disease, particularly in the case of cattle. It is imperative that the impact of
leptospirosis on abortion, stillbirths, and decreased milk production in livestock in the country be assessed. 相似文献
2.
A study was carried out to establish the prevalence of extra-intestinal porcine helminth infections and to assess the pig
slaughter slab sanitary conditions in Dar es Salaam city, Tanzania. A total of 24 privately owned pig slaughter slabs were
assessed. All slaughter slabs were sub-standard; wrongly located, poorly designed and constructed and lacked most basic requirements
for a slaughter house. Because of inadequate slaughtering, disposal and cleaning facilities, the slaughter slabs were under
unhygienic condition with questionable safety, soundness and wholesomeness of the pork produced. Routine meat inspection procedures
were used to detect extra-intestinal porcine helminth infections. Of the 731 examined pigs; 8.1%, 5.9% and 0.4% were infected
with ascariosis, porcine cysticercosis and hydatidosis, respectively. It was noted that almost all slaughter pigs in Dar es
Salaam originated from different regions. Based on the region of origin, the status of porcine cysticercosis was 8.2% for
Dodoma (n = 98), 8.2% for Manyara (n = 260) and 6.9% for Mbeya (n = 116). This study disclosed the unhygienic sanitary condition prevailing in Dar es Salaam pig slaughter slabs and recommends
that strategies should be devised to improve the situation. Porcine ascariosis and cysticercosis were widely prevalent and
caused economic losses due to condemnations. Because of their zoonotic nature, the observed extra-intestinal porcine helminth
infections in pig pose a public health risk among consumers. Thus, there is a need to introduce appropriate control measures
of parasitic infections in pigs. 相似文献
3.
Herd- and animal-level risk factors for bovine leptospirosis in Tanga region of Tanzania 总被引:1,自引:1,他引:0
Leptospirosis is the zoonosis of worldwide distribution and common cause of economic loss and ill health among animals and
human populations. A cross-sectional seroprevalence study, using a microscopic agglutination test (MAT) with a threshold titre
of ≥1:160, to elucidate disease magnitude, distribution and associated risk factors in cattle in Tanga, Tanzania was conducted
from May 2003 to January 2004. Serum (n = 655) samples collected from randomly selected herds (n = 130) were tested for antibodies against four different Leptospira interrogans serovars (Bataviae, Tarassovi, Hardjo and Pomona) used in the agglutination test. Positive titres were detected in 30.3%
[95% confidence intervals (CI) = 26.7–33.9] of cattle and 58.5% (95% CI = 49.5–67.1) of herds, respectively. Of the 198 MAT
positive serum samples, 98 (49.5%) were positive against serovar Hardjo, 80 (40.4%) were positive against serovar Tarassovi,
12 (6.1%) was positive against serovar Bataviae and eight (4%) were positive against serovar Pomona. Associations found to
be statistically significant in univariate analyses (at P < 0.1) were assessed by multivariable logistic regression to control for confounding factors. The results showed that risk
factors for cattle were pasture grazing [odd ratio (OR) = 2.83, 95% CI = 1.57–5.12, P = 0.001], presence of goats/sheep on the farm (OR = 1.73, 95% CI = 1.17–2.56, P = 0.001) and age of the animal (OR = 2.05, 95% CI = 1.42–2.96, P = 0.001), while concrete floor housing was protective (OR = 0.47, 95% CI = 0.30–0.74, P = 0.001). Herds managed under pasture grazing system were more likely to be sero-positive than those managed under zero grazed
practices (OR = 9.31; 95% CI = 3.67–23.64 for grazing herd). We concluded that bovine leptospirosis is an endemic and locally
widespread disease in Tanga and suggest that it may play a role in zoonotic transmission to humans. 相似文献
4.
Yuni Yupiana Peter R. Wilson Jenny F. Weston Emilie Valle Julie M. Collins‐Emerson Jackie Benschop Tim Scotland Cord Heuer 《Zoonoses and public health》2019,66(5):470-479
An epidemiological investigation was conducted in an unvaccinated dairy farming enterprise in which three workers on one of the milking herds (Herd 1) were diagnosed with leptospirosis due to serovars Hardjo (H) (n = 2) and Pomona (P) (n = 1) between January and March 2015. Blood and urine samples were collected from milking cows in Herd 1 (N = 230) and Herd 2 (N = 400), rising one‐ (R1, N = 125) and rising two‐year‐old (R2, N = 130) replacement heifers, and four pigs associated with Herd 1, in March 2015. Sera were tested using the MAT for serovars H, P, Copenhageni (C), Ballum (B) and Tarassovi (T), and urine samples were tested by qPCR. Seventy‐five per cent of 109 cows in Herd 1 and 36% of 121 in Herd 2 were seropositive (≥48), predominantly to H and P, and 23% of 74 cows in Herd 1 and 1% of 90 cows in Herd 2 were qPCR positive. Fifty‐five per cent of 42 R2 heifers were seropositive to T. No R1 and 17% of 42 R2 heifers were qPCR positive. Subsequently, all cattle were vaccinated for H and P, and Herds 1 and 2 were given amoxicillin. After the booster vaccination, 7% of 91 in Herd 1, 2% of 82 in Herd 2 and 11% of 38 R1 heifers (sampled as R2) were PCR positive. After the amoxicillin treatment, no cows in Herd 1 and 5% of 62 cows in Herd 2 were urine PCR positive. Calves and pigs were seropositive to H, P, C and B. Vaccination and antibiotic treatment appeared effective in reducing the risk of exposure of workers to vaccine serovars. However, evidence of non‐vaccine serovars indicated that workers likely remain at risk of exposure to Leptospira. 相似文献
5.
Sabri A. Rahman Kuan H. Khor Siti Khairani-Bejo Seng F. Lau Mazlina Mazlan Azri Roslan Soon H. Goh 《Journal of veterinary diagnostic investigation》2021,33(5):834
Leptospirosis is a serious bacterial disease that affects both humans and animals. A wide range of symptoms have been described in humans; the disease in dogs is commonly associated with kidney and/or liver disease. In Malaysia, information about the common serovars infecting dogs is limited. Therefore, we investigated the occurrences of leptospirosis in 124 pet dogs diagnosed with kidney and/or liver disease. Blood, urine, abdominal effusion, and/or kidney and liver were collected from the dogs. Based on microscopic agglutination testing, 53 of 124 (42.7%) dogs were seropositive for leptospiral exposure. Sera were frequently positive to serovars Bataviae (n = 12), Javanica (n = 10), and Icterohaemorrhagiae (n = 10). Direct detection using PCR showed that 42 of 124 (33.9%) of the whole blood and 36 of 113 (31.9%) urine samples were positive for pathogenic Leptospira spp. By PCR, 2 of 23 (9.1%) kidney and 2 of 23 (9.1%) liver were positive for pathogenic Leptospira spp. Abdominal effusion from 4 dogs were PCR-positive for pathogenic Leptospira spp. The species detected were L. interrogans, L. borgpetersenii, L. kirschneri, and L. kmetyi by partial 16S rRNA sequencing. We further identified and characterized 11 Leptospira spp. isolates from 8 dogs as serovars Bataviae, Javanica, and Australis. The mortality rate of the Leptospira-infected dogs was high (18 of 53; 34%). 相似文献
6.
Simeon I. B. Cadmus Mohammed K. Yakubu Abdullahi A. Magaji Akinbowale O. Jenkins Dick van Soolingen 《Tropical animal health and production》2010,42(6):1047-1048
Using deletion typing technique, five mycobacteria isolated from unpasteurised milk samples from cows in north-central Nigeria
were characterized as Mycobacterium bovis (n = 4) and M. africanum (n = 1). This report emphasizes that transmission between the animal and human reservoir is a serious threat in Nigeria. 相似文献
7.
Fernández-Silva JA Abdulmawjood A Akineden O Bülte M 《Tropical animal health and production》2011,43(8):1501-1507
The objective of this study is the detection of Mycobacterium avium subsp. paratuberculosis (MAP) by serum enzyme-linked immunosorbent assay (ELISA), fecal polymerase chain reaction (PCR), and fecal culture in Colombian
dairy herds. Serum and fecal samples from asymptomatic cows (n = 307) of 14 dairy herds were tested for MAP by an unabsorbed ELISA test (ELISA-A). Serum and fecal samples from positive
ELISA-A animals (n = 31) were further tested by an absorbed ELISA test (ELISA-B) and PCR. Fecal samples from animals of herds positive by ELISA-A
and PCR (n = 105) were inoculated onto three different culture media. ELISA-A produced positive results in 10% of the serum samples
and 71% of the herds. ELISA-B and PCR results were positive in two and six serum and fecal samples from positive ELISA-A animals,
respectively. Fecal samples were negative for MAP on all culture media. The results of this study confirmed the presence of
MAP in local dairy herds and the difficulties of MAP detection in asymptomatic animals by ELISA, PCR, and fecal culture. 相似文献
8.
el-Ashker MR 《Veterinary research communications》2011,35(5):311-320
The present study was carried out to evaluate the role of oxidative stress in the pathophysiologic process of acute renal
failure associated with exertional rhabdomyolysis (ER) in Egyptian horses. ER was tentatively diagnosed in 31 Baladi horses
based on case history, physical examination findings and confirmed by elevation of plasma creatine kinase (CK) and urine myoglobin
concentrations. According to severity of the condition, the diseased horses were categorized into two main groups; the first
group included 18 horses with minimal clinical signs and plasma CK <60 000 IU/L; whereas, the second group included 13 horses
with overt clinical signs and plasma CK >100 000 IU/L). It was found that plasma creatol (CTL) was positively correlated (p < 0.01) with plasma malondialdehyde (MDA) (r = 0.775), nitric oxide (NO) (r = 0.768), methyguanididne (MG) (r = 0.995), CK (r = 0.768), urine glucose (r = 0.778), urine protein (r = 0.767), renal failure index (RFI) (r = 0.814) and urine sodium (r = 0.799) and negatively correlated (p < 0.01) with total antioxidant capacity (TAC) (r = −0.795), superoxide dismutase (SOD) (r = −0.815), glutathione peroxidase (GSH-Px) (r = −0.675), Vitamin C (r = −0.830), urine creatinine (r = −0.800), urine/plasma creatinine ratio (r = −0.827) and urine/plasma urea ratio (r = −0.807). The correlation between these biochemical variables might suggest a possible role of oxidative stress in renal
injury associated with severe rhabdomyolysis in horses. It is suggested that exaggeration of oxidative stress associated with
increased muscle membrane leakage plays a key role in acute kidney injury in Baladi horses with severe rhabdomyolysis. 相似文献
9.
Dilmaghani M Ahmadi M Zahraei Salehi T Talebi A 《Veterinary research communications》2011,35(3):133-143
Salmonella enterica subspecies enterica serovar Typhimurium causes food-borne outbreaks and systemic diseases in humans and animals. groEL gene (also known as mopA gene in S. Typhimurium), possessing conserved sequence, plays an important role in invasion of bacteria. The purpose of present study
was to identify the polymorphism of groEL gene among different avians in different regions by PCR-RFLP method. Fifty two S. Typhimurium isolates (Broiler (n = 13), Layer (n = 12), Duck (n = 5), Goose (n = 5), Sparrow (n = 8), Canary (n = 3), Pigeon (n = 5) and Casco parrot (n = 1). were identified using serotyping as well as multiplex-PCR. Then, amplification of groEL gene performed and amplified products subjected to restriction digestion with BsuRI enzyme. Three RFLP profiles, A, B and
C, generated DNA fragments between approximately 100–1,000 bp in size, were observed. The RFLP profile A was observed in 35
(67.3%), profile B in 14 (26.9%) and profile C in 3 (5.77%) of isolates. S. Typhimurium isolates recovered from 13 broilers (two of which profile A, 9 profile B and 2 profile C) and from 8 sparrows
(two of which profile A, 5 profile B and 1 profile C) showed all three profiles, but 12 layers and other avians (including
Canary (n = 3), Goose (n = 5), Duck (n = 5), Pigeon (n = 5) and Casco parrot (n = 1)) showed profile A. None of these profiles was allotted for a special region. The result of present study showed that
S. Typhimurium undergoes genetic mutations in groEL gene under unpleasant milieu in different regions and in different avians. Thus, genetic diversity, despite conserved nature
of groEL gene in S. Typhimurium, may exist but it depends on the condition where bacteria have settled. To our knowledge, three RFLP profiles
of groEL gene generated by BsuRI restriction enzyme were not reported previously. 相似文献
10.
Nissen S Poulsen IH Nejsum P Olsen A Roepstorff A Rubaire-Akiiki C Thamsborg SM 《Tropical animal health and production》2011,43(3):567-572
During the last 30 years, pig production in Uganda and neighbouring counties has increased markedly. Pigs are mainly kept
as a source of income for small-scale farmers; however, the pig production is subject to several constraints, one of them
being worm infections. A study was carried out in rural communities in Kabale District in the South Western part of Uganda
in September and October 2007 in order to estimate the prevalence of gastrointestinal nematode parasites in pigs based on
coprological examination. Fifty-six households were randomly selected and visited. Housing system and deworming history were
recorded. Faeces was sampled from rectum of one to five pigs (age, 3–12 months) per household. A total of 106 pigs were examined
coprologically of which 91% excreted nematode eggs. The following prevalences of nematode eggs were recorded: strongyles (89%),
Ascaris suum (40%), Trichuris suis (17%) and spiruroid eggs (48%). On household level, rearing pigs on slatted floors in pens significantly reduced the faecal
egg excretion of strongyle eggs with almost 80% (p = 0.010) and a significant interaction between floor type and anthelmintic treatment was found for spiruroids (p = 0.037). Fifteen T. suis egg positive pigs were selected for post-mortem examination of the gastrointestinal tract. The post-mortem examinations revealed
that 93% pigs were infected with Oesophagostomum spp. (worm burden, min–max 10–2,180), 73% with A. suum (1–36), 67% with T. suis (6–58), and 20% with Hyostrongylus rubidus (worms not quantified). In general, nematode infections were widespread and polyparasitism common in pigs in Uganda. However,
worm burdens were moderate which may be related to recent deworming or to the practice of rearing pigs on slatted floors in
wooden elevated pens. 相似文献
11.
Robinson H. Mdegela Kibona Laurence Petro Jacob Hezron Emmanuel Nonga 《Tropical animal health and production》2011,43(1):83-87
Occurrences of thermophlic Campylobacter in pigs and pig carcasses was investigated in a cross-sectional study that was carried out in three selected slaughter slabs
in Morogoro municipality, Tanzania. Before sampling, the slab hygiene, slaughter, carcass dressing, and meat handling was
assessed. Fecal samples were collected from 66 slaughter pigs at the kill floor. After slaughter, a 100-cm2 area on medial surface of the thigh muscles of dressed carcasses was sampled using sterile cotton swabs. Thereafter, the
jejunal, cecal, and colon contents were also sampled. The samples were subjected to standard bacteriological examination using
Skirrows protocol. In all slaughter slabs visited, it was found that pig slaughter, dressing, and meat handling was done on
the ground under unhygienic condition. All the slaughter slab environment were dirty and had neither tap water or drainage
systems. Thermophilic Campylobacter prevalence in slaughtered pig was 66.7% while contamination rate of dressed carcasses was 10.6%. Of the Campylobacter-positive carcasses, five (12.2%) were from the animals which were also positive to Campylobacter. The isolation rate of Campylobacter in the cecum was higher (34.8%) compared to the small intestines (28.8%) and colon (16.7%) although the difference was not
statistically significant (P > 0.05). Campylobacter jejuni was the most prevalent species as it constituted 74% of all isolates, while Campylobacter coli was isolated at 26%. This suggests possible risks of infection to people through consumption of contaminated pork or through
contact with infected pigs. Cecum was found to be the major part of intestine highly colonized by Campylobacter. 相似文献
12.
A serological survey was conducted among sows in the Mekong delta in southern Vietnam in 1999 to investigate variations in
leptospiral Seroprevalence over a one-year period. In this region, leptospirosis is endemic and a high leptospiral Seroprevalence
has been shown in the pig population. In this study, the serology of six Leptospira serovars was analysed by the microscopic agglutination test for 429 sows at five large-scale state farms sampled during the
dry period, the rainy period and the early dry period. The serovars included were L. interrogans serovar (sv) autumnalis strain Akiyama A, L. interrogans sv bratislava strain Jez, L. interrogans sv icterohaemorrhagiae strain Kantorowicz, L. interrogans sv pomona strain Pomona, L. borgpetersenii sv tarassovi strain Perepelitsin, and L. kirschneri sv grippotyphosa strain Duyster. Variations in Seroprevalence over the year were found for sv bratislava and sv icterohaemorrhagiae:
the Seroprevalence was higher during the dry period compared with the rainy period (p = 0.07 and p = 0.005, respectively) and the early dry period (p = 0.00006 and p = 0.0006, respectively). It is concluded that in regions where water is constantly abundant and where animals are exposed
to the outdoor environment all year round there are highly significant variations in leptospiral Seroprevalence over the year. 相似文献
13.
Ferran Jori Hugo Galvez Patricia Mendoza Manuel Cespedes Pedro Mayor 《Research in veterinary science》2009,86(3):383-387
Leptospirosis, an endemic zoonoses, is maintained in the environment by several wildlife species in the Peruvian Amazon. In order to evaluate the possible role of collared peccaries (CP) in the maintenance this disease, two serological surveys of leptospirosis were performed and zootechnical parameters were monitored in a captive CP colony in an interval of 27 months. Total seroprevalence changed from 100% (n = 27) to 86.4% (n = 22), with reactions to a diversity of serogroups of zoonotic importance. Serological reactions to Leptospira licerasiae serogroup Iquitos, a new species recently identified locally and Leptospira interrogans serogroup Icterohaemorrhagiae were highly prevalent. The observation of leptospiral antibodies in both surveys, changes on serological reactions to different serogroups in large part of the herd and poor reproductive performances, provided an indication of the role of CP farms as a favourable environment for maintaining leptospirosis. Further research regarding the role of CP in the epidemiology of leptospirosis in the Peruvian Amazon is encouraged. 相似文献
14.
Chimana Henry M. Muma John Bwalya Samui Kenny L. Hangombe Benard M. Munyeme Musso Matope Gift Phiri Andrew M. Godfroid Jacques Skjerve Eystein Tryland Morten 《Tropical animal health and production》2010,42(7):1541-1545
A cross-sectional study was conducted between January 2007 and February 2008 to estimate seroprevalence of brucellosis and
identify risk factors associated with Brucella infections in commercial cattle in three districts of Lusaka province (Chongwe, Luangwa, and Kafue; n = 849) and in one rural district from the Central province (n = 48). A total of 897 serum samples were randomly collected from 55 farms along with animal-level data such as sex, age,
and parity. Sera were screened for presence of anti-Brucella antibodies using the Rose Bengal test, and positive samples were confirmed using competitive enzyme-linked immunosorbent
assay. At the animal level, seroprevalence was estimated at 7.9% (95% CI = 4.4–11.4%) in the Lusaka province and 18.7% (95%
CI = 7.5–29.9%) for Chibombo district. Brucellosis seroprevalence varied according to district, with Chongwe district recording
the highest compared to other districts. Seroprevalence also varied according to sex with bulls (n = 96) having higher seroprevalence (12.5%; 95% CI = 3.8–21.1%) compared to females (8.1%; 95% CI = 4.6–11.6). Similarly,
seroprevalence varied according to age groups, with the age category 1–4 years recording the highest (10.7%). The study recorded
relatively low Brucella seroprevalence in commercial farms in Lusaka, compared to the traditional small-scale farms. We suggest that testing and
stamping out of infected animals is likely to improve the situation and significantly reduce the public health risk associated
with Brucella infections in animals. 相似文献
15.
Growth performance and carcass characteristics of indigenous Mukota pigs of Zimbabwe 总被引:2,自引:2,他引:0
Chimonyo Michael Dzama Kennedy Mapiye Cletos 《Tropical animal health and production》2010,42(5):1001-1007
Carcass quality characteristics are essential in affecting grading and revenue that pig producers realize. No information
is available on the grading of carcasses in indigenous Mukota pigs of Zimbabwe. The objective of this study was to determine
the influence of genotype (Mukota and Large White × Mukota) and sex on post-weaning growth performance and carcass traits
of pigs fed maize cob-based diets. All pigs were weighed at 2-week intervals to estimate average daily gain, dressing percentage
(DP), and carcass lengths. Backfat thickness was also measured. Males had higher body weight gains than females (P < 0.05). Growth rates for Mukota (0.31 ± 0.010) and crossbred (0.41 ± 0.030) pigs were lower in the cool season than the
other seasons (P < 0.05). Body weights were consistently higher in the crossbred than in the Mukota pigs (P < 0.05). Mukota pigs showed a peak growth between 12 and 14 weeks post-weaning (P < 0.05). The DPs were 0.70 and 0.73 for the Mukota and crossbred pigs, respectively (P < 0.05). Crossbred pigs had longer (P < 0.05) carcasses than Mukota (655.5 ± 1.68 versus 507.2 ± 0.92 mm). Although there were no differences in the backfat thickness
between males and females of the crossbred pigs, Mukota males had thicker backfat than the females (P < 0.05). Crossbreeding is, therefore, significant in improving carcass grades of Mukota pigs. 相似文献
16.
Donald M. Myers Alfonzo Ruiz Lennox Applewhaite 《Tropical animal health and production》1985,17(4):239-243
Summary Cattle sera collected in the Republic of Guyana during 1981 to 1982 were examined for serologic evidence of leptospirosis.
Significant leptospiral agglutinins (1∶100 or higher) were disclosed in 49·1% of 2,935 apparently normal cattle from 734 farms
in the three major cattle-raising regions of the country. Seroreactions principally involvedhardjo andwolffi of the Sejroe serogroup,icterohaemorrhagiae, pomona, tarassovi, canicola andgrippotyphosa. A high proportion of reactions to one or more of different leptospiral serovars were observed in each of the three regions.
These variations were attributed to differences in environmental conditions and farming practices. The findings provided evidence
for the first time of the widespread occurrence of leptospirosis in cattle in Guyana caused by multiple leptospiral serovars.
Resumen Se examinaron sueros bovinos colectados durante 1981 y 1982, con el propósito de detectar aglutininas de leptospirosis. Mediante los análisis, se evidenció la enfermedad (titulos 1∶100 o superiores) en el 49,1% de 2.935 bovinos aparentemente sanos, en las tres principales regiones ganaderas del país. Las seroreacciones involucraron principalmente a lahardjo ywolffi del serogrupo Sejroe, lo mismo que a laicterohaemorrhagiae, pomona, tarassovi, canicola ygrippotyphosa. Se observó una alta proporción de reacciones a una o más serovars en cada una de las tres regiones estudiadas; estas se atribuyeron a prácticas de manejo diferentes, asi como a condiciones ambientales. El estudio, se concluye, proporcionó evidencia de la amplia distribición de la leptospirosis en Guyana.
Résumé On a recherché l'existence sérologique de la leptospirose dans des sérums de bovins de la République de Guyana récoltés en 1981 et 1982. Des agglutinines à un taux significatif (1∶100 et plus) ont été détectées chez 49,1% de 2935 animaux apparemment normaux répartis dans 734 fermes des trois grandes régions d'élevage du pays. Les réactions sérologiques touchent principalement les sérotypeshardio etwolfii du sérogroupe Sejroe,icterohaemorrhagiae pomona, tarassovi, canicola etgrippotyphosa. On note une importante proportion de réaction à un ou plusieurs des sérovars leptospiriens dans chacune des trois régions. Ces variations sont attribuées aux différences de conditions d'environnement et aux pratiques d'élevage. Pour la première fois, ces résultats apportent la preuve d'une répartition étendue de la leptospirose bovine en Guyana causée par plusieurs sérovars.相似文献
17.
The purpose of this study was to measure the dynamics of serum α1-acid glycoprotein (AGP) concentration in dogs with various
tumors, and to investigate the localization of AGP in some tissues using immunohistochemical staining. Sera were obtained
from 171 dogs bearing tumors of various types. Serum AGP concentration was measured by single radial immunodiffusion. Tumors
occurring in the liver and spleen were also investigated immunohistochemically using anti-canine AGP antibody. Mean serum
AGP levels were 749 ± 602 mg/L in dogs with carcinoma (n = 39), 1,014 ± 971 mg/L with sarcoma (n = 18), and 887 ± 935 mg/L with round cell tumors (n = 46), all significantly higher than serum AGP level in healthy dogs (n = 137, 364 ± 106 mg/L). Mean serum AGP levels were significantly higher than in healthy dogs in complex mammary gland carcinoma
(n = 5, 876 ± 721 mg/L), malignant melanoma (n = 7, 1,010 ± 821 mg/L), and hepatocellular carcinoma (n = 5, 936 ± 741 mg/L) among carcinomas, hemangiosarcoma (n = 5, 1,740 ± 1,323 mg/L) among sarcomas, and lymphoma (n = 19, 1,072 ± 965 mg/L) and histiocytic tumor (n = 6, 1,800 ± 1,387 mg/L) among round cell tumors. In an immunohistochemical investigation of AGP localization, both weak
and strong staining for anti-AGP antibody were seen in hepatic tissue in dogs with primary non-tumorous lesions originating
in the spleen (hematoma) and elevated serum AGP, but all tumor tissue in the spleen was negative. Among dogs with primary
tumor lesions of the spleen (hemangiosarcoma) and elevated serum AGP levels, both weak, moderate and strong staining for anti-AGP
antibody were seen in hepatic tissue, while strong positive staining was apparent in all tumorous tissue from the spleen.
In primary tumor lesions in the liver (hepatocellular carcinoma), both moderate and strong staining for anti-AGP antibody
were seen in normal hepatic tissue, and both weak, moderate and strong staining were seen in tumor tissues of the liver. AGP
levels thus appear to be elevated in dogs with carcinomas, sarcomas, and round cell tumors. With some of these malignant tumors,
localization of AGP in tumor tissue was seen. 相似文献
18.
Adrian Muwonge Clovice Kankya Jacques Godfroid Berit Djonne John Opuda-Asibo Demelash Biffa Takele Ayanaw Musso Munyeme Eystein Skjerve 《Tropical animal health and production》2010,42(5):905-913
To date, the public health relevance of mycobacterial infections in pigs is not well investigated despite high risk of infection.
Recently, there has been a documented increase in opportunistic infections and risk of acquiring opportunistic mycobacterial
infections in HIV/AIDS patients in Mubende district; unfortunately, there has been no published information on the epidemiology
of mycobacterial infections in this area. This study was carried out between September 2008 and February 2009. Investigations
were done to assess the prevalence and associated risk factors of mycobacterial infections in slaughtered pigs in Mubende
district of Uganda. A total of 997 pigs (53.7% male and 46.3% female) from 31 different slaughterhouses were examined for
the presence of lesions compatible with TB and mycobacterial infections. Pathologic tissue specimens were collected for culturing
and isolation of mycobacteria. A cross-sectional technique was used based on convenient visits to slaughterhouses but random
selection of individual slaughtered pigs for a detailed post-mortem inspection on a daily basis. The results reflected a 9.3%
and 3.1% (95% CI) prevalence of Mycobacterium species based on necropsy examinations and culture isolation, respectively. The highest prevalence of mycobacterial infection
was recorded in Buwekula County (the mixed agro-zone) whilst the lowest was in Kassanda County (pastoral zone). A multivariable
logistical regression analysis identified age (P ≤ 0.001) and sex (P ≤ 0.05) as risk factors for mycobacterial infections in pigs. Post-estimation statistics of the regression model evaluation
and validation fit it well into the data (HL, χ
2 = 5.9; P = 0.69 for necropsy, HL χ
2 = 2.9; P = 0.94 for culturing). This study documented a high prevalence of mycobacterial infections in slaughter pigs in Mubende district.
The fact that pigs and human often share common housing and environment poses a high risk of zoonotic transmission. This then
warrants further molecular investigation to identify the specific Mycobacterium species and their public health importance in this area. 相似文献
19.
Nutthee Am-in Wichai Tantasuparuk Mongkol Techakumphu 《Tropical animal health and production》2010,42(5):921-924
Following the implementation of artificial insemination (AI) services for smallholder pig farms, we investigated the reproductive
performance after AI and its influencing factors. A small-scale boar station with an AI lab was established with two active
boars having good genetics and free from reproductive diseases. Individual sow cards were used for reproductive data recording.
A total of 171 sows on 92 farms situated within a radius of 50 km from the AI center were included in this study. Sows bred
by AI (n = 121) were inseminated twice per estrus by two trained inseminators. A further 50 sows were mated by natural services using
local rental boars. The impact of boar stimulation and distance from the AI center to the farm were also determined. Non-return
(P = 0.02) and farrowing rates (P = 0.03) were higher for AI than for naturally bred sows (84.0% and 76.0% vs. 74.0% and 70.0% for AI and naturally bred, respectively).
For sows bred by AI, boar stimulation increased non-return rate (84.1% vs. 70.0%; P = 0.09), farrowing rate (83.7% vs. 69.2%; P = 0.01) and litter size (11.2 ± 2.3 vs. 9.7 ± 1.7; P < 0.01). There was no effect on performance due to distance of semen transport. These results clearly indicate that sow performance
on smallholder farms will improve if AI is utilized and boar stimulation is employed. 相似文献
20.
Ghavi Hossein-Zadeh N 《Tropical animal health and production》2012,44(3):531-536
The objective of the present study was to estimate genetic changes of body weight at different ages in Moghani sheep. Traits
included were birth weight (BW, n = 4,208), 3-month weight (3MW, n = 4,175), 6-month weight (6MW, n = 3,138), 9-month weight (9MW, n = 2,244), and yearling weight (YW, n = 1,342). Data and pedigree information used in this study were collected at the Breeding Station of Moghani sheep during
1989–2005. The analysis was carried out for five traits, using the MTGSAM program. Breeding values of individual animals were
obtained from a multivariate animal model analysis and genetic trends were obtained by regressing the means of predicted breeding
values on year of birth for each trait. Direct genetic trends were positive and significant (P < 0.05) for BW, 3MW, 6MW, 9MW, and YW and were 1.63, 69.20, 79.38, 66.83, and 110.22 g/year, respectively. Also, maternal
genetic trends for BW, 3MW, 6MW, 9MW, and YW were positive and significant (P < 0.05) and were 2.36, 49.18, 37.33, 17.73, and 9.67 g/year, respectively. The results showed that improvement of body weights
of Moghani sheep seems feasible in selection programs. 相似文献