Mid-piece length of spermatozoa in different cattle breeds and its relationship to fertility

Recently positive correlation has been found between oxygen consumption (ZO2) in bull spermatozoa and non-return rates and concluded that an increase in ZO2, characteristic of the freeze/thaw process, was possibly associated with mitochondrial membrane damage during this procedure: alternatively, sperm may be hyperactivated through the capacitation-like effects of freezing/thawing. We speculated that the morphology of spermatozoa may be associated with their rate of ZO2 and fertility: for example, sperm mid-piece length where mitochondria are located. Such a relationship has not been investigated before, particularly in context of commercial cattle breeding programmes and bull fertility characteristics. Sperm biometry was performed on ejaculates obtained from 34 bulls representing six breeds: Holstein (yearlings and mature), Friesian, Belgian Blue, Aberdeen Angus, Charolais and Limousin. Five ejaculates were collected from every bull and from each sample a semen smear was fixed and stained with eosin/nigrosin: the mid-piece length of 40 sperm with normal morphology was measured in every sample. Data were analysed by breed, age and within each bull. Significant differences (p<0.01) between ejaculates in 9/34 bulls was found, as well as differences (p<0.001) between individual bulls within the same breed. The average mid-piece length for Aberdeen Angus was 13.35 microm, for Belgian Blues and Limousin around 13.8 microm and for Charolais 13.68 microm: for dairy breeds such as Holstein and Friesian it was about 13.4 microm. The mean value of mid-piece length for breed was compared with their 49 day non-return rate; a negative correlation (r = -0.53) was found in black and white dairy breeds.     

Comparison of vitamin D deficiency and secondary hyperparathyroidism in obese and non-obese children and adolescents

Obesity subjects individuals into metabolic and endocrine disorders. Thus obesity may increase the risk of vitamin D deficiency. This text aims at studying the prevalence of vitamin D deficiency and secondary hyperparathyroidism in obese children. In a non-randomized case control study on 52 obese children (body mass index (BMI) >95th percentile) aged 4 to 16 years undertaken at the outpatient endocrine clinic of the Children Hospital at Tabriz University between 2009-2011. This study was conducted to compare the prevalence of vitamin D deficiency and secondary hyperparathyroidism in obese children compared with 57 non obese (BMI < 85th percentile). 109 children including 52 (50.5%) boys and 57 (49.5%) girls were studied. Most of case (76.9%) and control (42.1%) groups suffered from degrees of vitamin D deficiency. There was meaningful statistical difference between two groups considering to vitamin D deficiency and parathyroid hormone (p = 0.001). A negative relations was found between iPTH and vit D level (p < 0.001, r = -0.2), BMI and 25-OH vit D (p < 0.001, r = -0.2). A positive relation was observed between parathyroid hormone and BMI (p = 0.009, r = 0.1). Obese children are at high risk at vitamin D deficiency and secondary hyperparathyroidism. BMI appears to be an important risk factor for vitamin D deficiency.     

Herbage production and quality of a range of secondary grass species at five rates of fertilizer nitrogen application

The productivity of ten grasses was measured under six cuts per annum for 3 years and three cuts for a fourth harvest year in comparison with a perennial ryegrass (Lolium perenne) control. The grasses were Yorkshire fog (Holcus lanatus), two red fescues (Festuca rubra), creeping bent (Agrostis stolonifera), bent (Agrostis castellana), bent (Agrostis capillaris), smooth meadow-grass (Poa pratensis), crested dogstail (Cynosurus cristatus), sweet vernal (Anthoxanthum odoratum) and timothy (Phleum bertolonii). Another grass sown, rough meadow-grass (Poa trivialis), did not persist after the first harvest year. Annual N rates were 0, 120, 240, 360 and 480 kg ha^?1. Classified by DM production, the grasses grouped into: high (perennial ryegrass, Yorkshire fog and red fescue), intermediate (creeping bent, bent (A. castellana), crested dogstail and smooth meadow-grass) and low (bent (A. capillaris), sweet vernal and timothy. With frequent cutting, production ranking interacted with N rate: Yorkshire fog. red fescue and creeping bent were more productive than ryegrass at the 0 N and 120 N rates but not at higher N rates; crested dogstail and sweet vernal outperformed ryegrass at 0 N; ryegrass and smooth meadow-grass performed relatively better at moderate to high N. DM production of ryegrass was matched or exceeded by some of the other grasses during different parts of the season particularly at low N rates. Notable features from the 3-cut regime were the outstanding DM production of Yorkshire fog at all N rates and the poor production of bent (A. capillaris) and crested dogstail. High DM response to applied N in both cutting regimes was given by perennial ryegrass. smooth meadow-grass, red fescue cv. Boreal, timothy, crested dogstail and Yorkshire fog. N concentrations were highest in less productive grasses such as the bents and lowest in highly productive ryegrass and Yorkshire fog. Ryegrass was superior in digestibility to the other grasses, but especially to the red fescues, bents and smooth meadow-grass. The other grasses, especially Yorkshire fog and bent (A. capillaris) were superior to ryegrass in P, K and Mg concentrations, but not Ca. It is concluded that the annual and/or seasonal production of some of the secondary grasses at low to moderate N rates, e.g. the performance of Yorkshire fog and red fescue, warrant a reconsideration of their poor reputation, particularly within the context of increasing grassland extensification. Breeding to improve selected characteristics, such as digestibility in red fescue, seems justified. Assessment of the grazing and ensiling qualities of secondary grasses require further study.     

Morphometrical study of polysialylated neural cell adhesion molecule positive cells in rat pups hippocampus following induction of seizure during pregnancy

Background:The polysialylated neural cell adhesion molecule (PSA-NCAM) is expressed in developing brain. Fetal brain damage is caused by different conditions such as seizure and hypoxia. The present study was designed to investigate the effect of maternal seizures on the number of PSA-NCAM positive cells in pup''s hippocampus. Methods: Female Wistar rats were divided into four groups: (a) kindled rats which received PTZ (40 mg/kg, i.p.) during pregnancy from embryonic day 14-19 (E14-E19) every 48 h, (b) kindled rats which did not receive PTZ during pregnancy, (c) non-kindle, pregnant rats which received PTZ injection (40 mg/kg, i.p.) during pregnancy from E14 to E19 every 48 h, and (d) non-kindle, pregnant rats which received injection with an equal volume of normal saline as sham controls. At postnatal day 14 (PD₁₄), rat pups were perfused, and their brain were fixed, embedded and coronal sections stained by immunohistochemistry method. The number of PSA-NCAM positive cells per unit area in the pup''s hippocampus was counted. Results: The number of PSA-NCAM positive cells in the CA1, CA3, and DG fields of pup''s hippocampus, which was obtained from mothers who experienced PTZ injection during pregnancy, was decreased approximately 2.6 (P = 0.001), 2 (P = 0.001), and 2.1 (P = 0.001) times compared with non-PTZ treated maternal groups, respectively. Conclusions: Our study showed that maternal seizures reduced the number of neurons and also PSA-NCAM positive cells per unit area in the offspring hippocampus that it may cause impairment in hippocampal functions.Key Words: Epilepsy, Polysialylated neural cell adhesion molecule (PSA-NCAM), Hippocampus, Seizures     

小麦品种面粉白度的变异及其影响因素分析

为提高小麦品种的面粉白度,以来自不同种植区的216个小麦品种(系)为材料,研究了小麦面粉白度的分布规律及与其他品质指标的相关性,并初步探讨了储藏时间对面粉白度的影响。结果表明,面粉白度在小麦品种(系)间存在极显著差异(P0.01),年份间极显著正相关;参试材料的面粉白度平均为72.9,变幅为54.1~80.1,高于80的品种较少,大多数品种(系)的白度值在70~75之间。来源于西南冬麦区的小麦品种(系)的面粉白度值最高,显著高于黄淮冬麦区和北方冬麦区(P0.05),长江中下游冬麦区的白度值也较高;国外引进品种的白度值普遍较低,平均值为69.53;软麦的白度值显著高于硬麦(P0.05)。面粉白度与出粉率、籽粒硬度、蛋白含量和粉质参数等指标均呈负相关,尤其与硬度的负相关性最强(r=-0.798)。储藏一段时间可略微增加面粉白度,但增幅不明显。同时,筛选出一批优质高白度材料,可作为培育强筋高白度小麦品种的优良亲本。     

基于转录组的荔枝ARF基因家族的鉴定及表达分析

生长素反应因子(Auxin Response Factors,ARFs)是调节生长素表达的转录因子响应基因,ARF基因在植物中大多由多基因家族组成。基于转录组数据,通过生物信息学方法对荔枝ARF基因进行鉴定,并分析其理化性质、亚细胞定位、保守基序、系统进化以及基因的表达模式。在荔枝中鉴定出21个ARF基因,其编码的蛋白质含有53~1 117个氨基酸,分子量约为6.112~123.872 ku,等电点为4.21~9.45。亚细胞定位预测结果显示21个ARF均定位于细胞核。Lc ARFs基因家族具有相对保守的结构,即包含1个保守的B3 DNA结构域、ARF结构域和Aux/IAA结构域。进化树分析表明荔枝ARF蛋白分为5个亚家族。21个荔枝ARF基因在花穗发育过程中有明显不同的表达规律。该结果为进一步深入研究荔枝ARF基因家族的功能奠定了基础。     

The apparent digestibility of energy,nitrogen and fibre and the biological value of protein in low- and high-fibre wheat breads

Sixteen 15 kg liveweight entire-male pigs were given either a low-fibre (1.21 g/100 g Neutral detergent fibre, NDF) or a high-fibre (6.38 g 100 g, NDF) wheat bread as their sole source of dietary protein, in a conventional 21-day metabolism study. A glucose/oil supplement which was assumed to be completely absorbed was given with the bread to ensure a high ratio of dietary energy to protein, to allow measurement of biological value (BV). The apparent faecal digestibility of gross energy was significantly (p<0.001) lower (7.4% units) for pigs given the high-fibre bread as was the apparent digestibility of NDF (24% units lower). The apparent faecal digestibility of total nitrogen was also significantly (p<0.001) lower for the animals fed the high-fibre bread, but there were no significant differences between the breads for the BV of their protein. The overall mean BV for the breads was 46%. The results indicate a significantly lower digestibility of nutrients and gross energy in breads containing appreciable quantities of wheat bran fibre.     

Effect of breed of cattle and season on diet selection and defoliation of competitive plant species in semi-natural grasslands

To maintain biodiversity in semi‐natural grasslands, the choice of breed of livestock and season of grazing can be important. The objective was to determine the effects of breed and season on the composition of plant fragments in the faeces of cattle grazing a heterogeneous semi‐natural grassland. Twelve heifers of the traditional Swedish Väneko breed (live weight 309 kg) and twelve heifers of the Charolais breed (live weight 431 kg) were allocated to three pastures per breed. Faeces samples were collected in spring, summer and autumn for micro‐histological examination of plant fragments. On the same occasion, defoliation of four competitive plant species was recorded. Faecal plant fragments mainly consisted of grasses but also herbs, whereas Cyperaceae, despite their abundance on the pastures, only composed a small proportion of fragments. Season affected the composition of plant fragments in the faeces, with a higher proportion of Cyperaceae and herbs in spring than later in the grazing period (P < 0·001), and the heifers ingesting more grasses and woody plants in autumn. In spite of a clear difference in live weight between the two breeds, no effects of breed on the composition of plant fragments in faeces were observed, although Väneko heifers defoliated more Filipendula ulmaria in spring than the Charolais heifers (P < 0·01). In addition, defoliation of Alnus glutinosa increased later in the grazing period (P < 0·05), especially for the Väneko heifers, indicating control of pernicious brushwood by grazing may be more effective in autumn.     

Dietary Onion Intake as Part of a Typical High Fat Diet Improves Indices of Cardiovascular Health Using The Mixed Sex Pig Model

The aim of this study was to evaluate the potential health benefits of onions consumed at two levels of intake, using the pig model. The dietary fat content was set at a level typical of a “western” diet (25% w/w). Fifteen female and fifteen male pigs (Large White × Landrace) were allocated to one of three dietary treatments in a randomised block design. Treatments consisted of control diet (no onion) and onion supplementation at either 8.6 or 21.4 g of onion/MJ DE fed for six weeks. Onion consumption reduced plasma triglyceride levels by 15% (P=0.030) regardless of sex and onion dose. Total plasma cholesterol and cholesterol fractions were unaffected by onion supplementation (P > 0.050). The bioactivity of onion was evident in haematocrit measures, where red blood cell and haemoglobin were significantly reduced in a dose dependant manner (P < 0.001 and P=0.011, respectively), while other cell counts, with exception of segmented neutrophils (−18%, P=0.012), were largely unaffected. Serum oxidative status was improved (P=0.007) in pigs consuming onions. These data demonstrate that consumption of onions can have positive health effects in both male and female pigs consuming a high fat diet.     

The implications of controlling grazed sward height for the operation and productivity of upland sheep systems in the UK. 3. The effect of choice of genotype, level of nitrogen fertilizer and lamb:ewe ratio

Grazed sward surface height was controlled within the range 3·25–4·75 cm during spring and summer in two experiments. In Experiment 1, the effects of stocking two breeds of ewe of similar size but different potential levels of reproductive performance [Brecknock Cheviot (C) and Beulah Speckled Face (B)] at different annual stocking rates of twelve (SR₁₂) and twenty (SR₂₀) per hectare, rates of nitrogen fertilizer of 100 (N₁₀₀) and 200 (N₂₀₀) kg N ha^?1 annum^?1 and different lamb:ewe ratios (C_1·2, B_1·2 and B_1·5) were measured in four treatments (SR₂₀N₂₀₀C_1·2; SR₂₀N₂₀₀B_1·2; SR₂₀N₂₀₀B_1·5; SR₁₂N₁₀₀C_1·2) replicated three times. In each of three years animal performance and yield of silage from areas of pasture surplus to grazing requirements were measured. In Experiment 2, breed B was compared with the Welsh Mule (W) breed, a larger with a higher potential reproductive performance, at two stocking rates, two rates of nitrogen fertilizer and two lamb:ewe ratios set on the basis of results from Experiment 1 (SR₁₈N₂₀₀B_1·5; SR₁₂N₁₀₀B_1·5; SR₁₈N₂₀₀W_1·5; SR₁₈N₂₀₀W_1·7). The treatments were replicated three times. The same terminal sire (Suffolk) was used in both experiments. A primary aim of the experiments was to test the validity of the experimental procedures used for comparing breeds of sheep where nutrition is provided predominately from grazed pastures. In Experiment 1, there was no difference between breeds C and B in the live weights of individual lambs at weaning at the same SR (20), N rate (200) and lamb:ewe ratio (1·2). Breeds C and B produced similar total yields of lamb (633 kg lamb ha^?1± 10·5) and silage (193 kg DM ewe^?1± 37·7), but breed B had a higher level of potential reproductive performance (1·59 vs. 1·37 lambs ewe^?1: P < 0·001). The treatments SR₂₀N₂₀₀B_1·5 and SR₁₂N₁₀₀C_1·2 produced, respectively, greater and lesser yields of lamb (725 vs. 384 kg lamb ha^?1, P < 0·001) and lesser and greater yields of silage (123 vs. 327 kg DM ewe^?1, P < 0·001). In Experiment 2, the live weight of lambs at weaning from breed W were heavier than from breed B (29·1 vs. 26·2 kg lamb^?1, P < 0·01) but there was no significant difference in total yield of lamb weaned between breeds W and B at the same SR (18), N rate (200) and lamb:ewe ratio (1·5) (747 kg lamb ha^?1± 19·2), or in the yield of silage (66 kg DM ewe^?1± 16·4), but breed W had a higher potential reproductive performance (1·85 vs. 1·58 lambs ewe^?1, P < 0·05). The treatments SR₁₈N₂₀₀W_1·7 and SR₁₂N₁₀₀B_1·5 produced, respectively, greater and lesser yields of lamb (840 vs. 473 kg lamb ha^?1, P < 0·001) and similar and greater yields of silage (60 vs. 141 kg DM ewe^?1, P < 0·05). The experimental approach adopted and the management protocols used provided a basis for ranking the performance of the breeds of ewes examined at appropriate levels of annual stocking rate, N-fertilizer input and lamb:ewe ratio.     

Pre- and post-McDonald cerclage cervical length, width and funneling rate and their association with duration of pregnancy

Changes of cervical ultrasonographic physical parameters and their association with outcome of cerclage have not been fully elucidated. This study aimed at evaluating association between ultrasonographic physical cervical shapes before and after McDonald cerclage with the length of pregnancy. Cervical length, width and funneling rate were assessed by transvaginal ultrasonography before and after the procedure. Mean pre-cerclage cervical length, width and funneling rate were 29.28 +/- 5.54, 19.92 +/- 10.30 mm and 12.90 +/- 18.87%, respectively. The mean post-cerclage cervical length, diameter and funneling rate were 31.68 +/- 4.21, 9.78 +/- 13.86 and 4.32 +/- 10.89%, respectively (p < 0.001 for all pre- and post-cerclage changes). The mean post-cerclage duration of pregnancy was 19.78 +/- 5.94 weeks. There were significant direct correlations between the pre-and post-cerclage cervical lengths with pregnancy duration (r = 0.24, p = 0.04 and r = 0.44, p < 0.001, respectively). There were significant indirect correlations between the pre- and post-cerclage funneling rate with pregnancy duration (r = -0.30, p = 0.01 and r = -0.20, p = 0.01, respectively). Similar correlations were not significant for the cervical widths. This study showed that the pre- and post-cerclage ultrasonographically determined cervical length and funneling are good predictors of pregnancy duration.     

Antioxidant status of bilirubin and uric acid in patients diagnosed with Plasmodium falciparum malaria in Douala

Oxidative stress and changes in antioxidant status have been implicated in the pathogenesis of malaria. To assess the antioxidant level ofbilirubin and uric acid associated with falciparum malaria infection, 60 untreated patients (30 men and 30 women) in Douala, Cameroon were screened for the study. Sixty five healthy individuals (29 men and 36 women) were used as controls. Total and conjugated bilirubin were calculated using Jendrassik-Grof method while uric acid was determined using Barham-Trinder method. It was observed that total and conjugated bilirubins were significantly (p < 0.001) higher in malaria patients (10.722 +/- 4.043 and 3.627 +/- 1.571 mg L(-1), respectively) when compared to control (6.830 +/- 2.436 and 1.777 +/- 0.729 mg L(-1)) and these bilirubin levels increased significantly with parasite count (p < 0.050). There was also significant increased (p = 0.021) of uric acid in malaria patients (56.262 +/- 13.963 mg L(-1)) compared to controls (49.838 +/- 15.419 mg L(-1)). No significant differences based on sex were observed on uric acid, parasite count, total and conjugated bilirubins in malaria patients. Positive correlations were obtained between parasite count and total bilirubin (r = 0.320, p < 0.050), conjugated bilirubin (r = 0.477, p < 0.001), uric acid (r = 0.060, p > 0.050) and between total and conjugated bilirubin (r = 0.729, p < 0.001). From this study, it has been hypothesized that the augmentation of plasma level ofbilirubin and uric acid could provide more protection against oxidative stress induced by malaria.     

Potato Shoot Tip Cryopreservation. A Review

Potato is one of the most important crops worldwide. Genetic resources of potato (Solanum tuberosum L. ssp. tuberosum) and related cultivated species are conserved through storage of tubers, in vitro plants and in cryopreservation. Cryopreservation, storage in or above liquid nitrogen, is the best option to maintain vegetatively propagated plants in the long term. The present review gives comprehensive information about various cryopreservation techniques for potato published from 1977 until the present. It discusses factors that affect the process and success of cryopreservation, such as donor culture conditions, preculture, cooling, warming and post-culture treatments. Studies are presented that analyse the histological and ultrastructural changes after different cryopreservation steps and the morphological pathways during regeneration of plants after rewarming. The maintenance of genetic stability in potato after cryopreservation has also been demonstrated by various phenotypic and molecular methods. The first thermal analyses on potato shoot tips are presented using differential scanning calorimetry to analyse the state of water during cooling and warming. Biochemical analyses of different compounds, such as soluble sugars and proteins, have been performed to understand and improve existing cryogenic methods. Potato is an example where successful virus elimination has been obtained via cryopreservation of shoot tips (cryotherapy). There are already cryopreserved collections of potato shoot tips in Germany, Peru, Czech Republic, South Korea and USA, but additional experiments on fundamental aspects of potato cryopreservation will help to improve understanding of the different cryopreservation methods, start new collections in other countries and also build up existing cryocollections of potato.     

Anthocyanins Delay Ageing-Related Degenerative Changes in the Liver

Liver ageing is a significant risk factor for chronic liver diseases. Anthocyanin is a food additive that has previously shown efficacy in increasing longevity. Here, we tested whether anthocyanins could protect young mice from accelerated ageing of the liver. Kunming mice were injected with D-galactose to accelerate ageing and were given 20 or 40 mg/kg anthocyanins as an intervention. After eight weeks, whole liver function and structure were evaluated, and the expression levels of genes involved in the DNA damage signalling pathway were assessed by Western blot analysis. Anthocyanins delayed the reduction of the liver index (p < 0.05), hepatic tissue injury and fibrosis. Anthocyanins also maintained the stability of the redox system (GSH-PX, T-SOD and MDA) in plasma and liver structures (p < 0.001) and reduced the levels of inflammatory factors (IL-1, IL-6 and TNF-α) in the liver (p < 0.05). Moreover, the expression levels of sensors (ATM and ATR), mediators (H2AX and γ-H2AX) and effectors (Chk1, Chk2, p53 and p-p53) in the DNA damage signalling pathway were all reduced. Anthocyanins could be widely used in the field of health products to slow ageing-related deterioration of liver function and structure by inhibiting DNA damage.     

Impact of ABCB1 Gene Polymorphisms and Smoking on the Susceptibility Risk of Chronic Myeloid Leukemia and Cytogenetic Response

Background:IM, a strong and selective TKI, has been approved as the front line of treatment in CML patients. In spite of satisfactory results of imatinib in the treatment of patients with CML, patients with treatment failure or suboptimal response developed resistance that might be because of pharmacogenetic variants. This study attempted to evaluate the influence of ABCB1 gene polymorphisms and smoking on CML risk and resistance to imatinib. Methods: ABCB1 (c.1236C>T, c.3435C>T) polymorphisms were genotyped in 98 CML patients and 100 sex- and age-matched healthy subjects by PCR-RFLP method, followed by sequencing. The patients were evaluated for cytogenetic response by the standard chromosome banding analysis in regular intervals. Results:Our results showed that c.1236CC genotype was significantly associated with imatinib resistance (OR = 3.94; p = 0.038). Analysis of the joint of SNP-smoking combination showed that smokers with c.1236TT/CT and c.1236CC genotypes had the increased risk of CML (OR = 6.04; p = 0.00 and OR = 4.95, p = 0.005) and treatment failure (OR = 5.36, p = 0.001 and OR = 15.7, p = 0.002), respectively. Smokers with c.3435TT/CT and c.3435CC genotypes also displayed the elevated risk of CML development (OR = 6.01, p = 0 and OR = 4.36, p = 0.011) and IM resistance (OR = 5.61, p = 0.001 and OR = 13.58, p = 0.002), respectively. Conclusion:Our findings suggest that c.1236CC genotype has clinical importance in the prediction of treatment outcome with IM, and smoking could have a synergistic role in CML risk and IM resistance. Key Words: ATP binding cassette transporter subfamily B, Imatinib mesylate, Smoking     

6种草本药用植物种子超低温保存技术研究

以草本药用植物杜若、过江藤、夏枯草、皱果苋、罗勒和山香的成熟种子为材料,探讨了含水量和冷冻方法对种子超低温保存的影响。结果表明,经液氮超低温冷冻后,6种草本药用植物种子发芽率较对照组均有显著差异(p0.05);适宜的含水量下,种子经过超低温冷冻后其发芽率甚至高于对照组。3种冷冻方法中,玻璃化冷冻法更适合过江藤和山香种子的超低温保存,缓慢冷冻法更适合皱果苋、杜若和夏枯草种子的超低温保存,直接冷冻法适合于杜若和罗勒种子的超低温保存。所以,液氮超低温冷冻法保存杜若等6种草本药用植物种子是可行的;含水量对超低温保存前后夏枯草、山香和罗勒种子的发芽率影响显著。     

Pregnancy rate following transfer of in vitro produced lamb derived embryos in two embryonic stages

Ovine embryos were produced by maturation, fertilization and in vitro culture (IVM/IVF/IVC) of oocytes collected from slaughtered prepubertal ewes. At 24 h post IVM, oocytes were fertilized with fresh semen collected from Lori-Bakhtiari breed at a concentration of 1.0 x l0(6) sperm mL(-1). The presumptive ova/embryos were transferred into the embryo culture medium at 22-24 h post IVF. Following 4 to 7 day in culture, embryos (at morula and blastocyst stage, respectively) were transferred surgically to the uterine horn of synchronized recipients. Pregnancy was diagnosed at day 30 by hormonal assay and at days 55 and 140 of gestation by ultrasonography and pregnancies were allowed to go to term. A total of nine ewes received 27 embryos (3 embryos/ewe). Five ewes received 15 embryos at morula stage and four ewes received 12 embryos at blastocyst stage. From those received morula stage embryos one was pregnant on day 30 (20%), though no pregnancy was diagnosed on each of days 55 and 140. While from those received blastocyst stage embryos, three ewes were pregnant on day 30 (75%) and two ewes (50%) remained pregnant on each of days 55 and 140. In conclusion, day 4 IVM-IVF morula stage embryos had a lower survival rate than did day 7 IVM-IVF blastocysts embryos, following transfer to the synchronized recipient ewes.     

Arthroscopically-assisted vs. open surgery in repairing anterior cruciate ligament avulsion

The management of an Anterior Cruciate Ligament (ACL) avulsion is principally by surgery. There are two major techniques in this regard; fixation through open surgery (arthrotomy) or arthroscopically-assisted repair. These are not new in the literature; however, the debate regarding the better approach is still under debate. This study focused on comparing outcome and consequences of arthroscopically-assisted vs. open surgery in repairing ACL avulsion. In a randomized clinical trial, 44 patients with unilateral ACL avulsion were recruited in Tabriz Shohada teaching Centre during a 12 month period of time. These patients were randomized in two equal age and sex-matched groups underwent either arthroscopically-assisted or open ACL repair. The constructive technique was the same in both groups without using any graft. The minimum follow-up period was 6 months including 3 weeks, 3 months and 6 months postoperatively. Range of Motion (ROM), laxity, Anterior Drawer Test (ADT) result, duration of hospital stay, wound status, nonunion and return to previous work were compared between the two groups. Mean time of suture removal (p = 0.10), minimum and maximum ROM at months 3 (p = 0.43 and 0.22, respectively) and 6 (p = 0.73 and 0.77, respectively), ADT at months 3 and 6 (p = 0.16 for both), laxity at month 6 (p = 0.28) and wound status at week 3 (p = 0.35) were not significantly different between the two groups. There was no case of nonunion at month 6 in either group. The mean hospital stay was significantly shorter in the arthroscopy group (3.77 +/- 0.92 vs. 2.50 +/- 0.51 days; p < 0.001). The mean laxity score was significantly higher in the open surgery group at month 3 (4.82 +/- 0.59 vs. 4.45 +/- 0.60; p = 0.05). The rate of nonunion was significantly higher in the open surgery group at month 3 (40.9% vs. 9.1%; p = 0.02). Patients in the arthroscopically-assisted technique group returned sooner to previous work (17.41 +/- 1.53 vs. 14.82 +/- 0.96 weeks; p < 0.001). Based on our results, arthroscopically-assisted reconstruction of ACL avulsion is superior to open surgery.     

Effect of flax seeds and date palm leaves extracts on serum concentrations of glucose and lipids in alloxan diabetic rats

The aim of the present investigation was to examine the effect of Flax Seeds (FS) and Date Palm Leaves (DPL) extracts on the concentration of serum glucose and lipids in alloxan diabetic rats. Rats were divided into six groups, normal control rats (without treatment with either FS or DPL), normal control rats treated with either FS or DPL extract for four weeks, diabetic control rats and diabetic rats treated with either FS or DPL extract for four weeks. The concentration of glucose in diabetic rats treated with FS (D+FS) for four weeks was significantly decreased from 17.20 +/- 2.33 to 8.14 +/- 0.54 mmol L(-1) (p < 0.001). Serum total cholesterol concentration (TC) of diabetic rats treated with either FS or DPL extract showed significant decrease by 40 and 31% respectively in the fourth week. Also, serum low density lipoprotein cholesterol (LDL-C) concentration was significantly decreased after two weeks of treatment (p < 0.001 and p < 0.01, respectively). However, no significant changes were obtained in serum high density lipoprotein cholesterol (HDL-C) concentration. Results of the present study showed that FS extract has a hypoglycaemic effect against alloxan diabetic rats. Present findings also showed that treatment of alloxan diabetic rats with either FS or DPL extract significantly decreased serum concentration of TC and LDL-C. Present findings suggest that both of FS and DPL extracts could have a protective effect against diabetes complications as well as against hyperlipidemia through improvement of lipid profile.     

Effects of ovariectomy or orchidectomy and estradiol valerate or testosterone enanthate replacement on serum insulin in rats

Various clinical observations and experimental data from in vitro studies suggest that insulin and sex hormones interact. The main purpose of the present study was to examine the effects of testosterone and estradiol on serum insulin in rats. Seven week old male and female albino (Wistar) rats were used in our study. Testosterone enanthate (50 mg kg(-1) day(-1)) or estradiol valerate (200 microg kg(-1) day(-l)) were injected intraperiotenally or subcutaneously in orchidectomised or ovariectomised rats, respectively. In orchidectomised rats, serum insulin was decreased compared with control animals (p < 0.01), on the other hand, decreasing of serum insulin was prevented by testosterone replacement (p < 0.001). In ovariectomised rats, serum insulin was also decreased compared with control group (p < 0.01) and decreasing of serum insulin was prevented by estradiol replacement (p < 0.05). Conclusively, present findings indicated that testosterone or estradiol were serum insulin enhancer hormones in male or female rats, respectively.