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QTL mapping for seven quality traits was conducted by using 254 recombinant inbred lines (RIL) derived from a japonica-japonica rice cross of Xiushui 79/C Bao. The seven traits investigated were grain length (GL), grain length to width ratio (LWR), chalk grain rate (CGR), chalkiness degree (CD), gelatinization temperature (GT), amylose content (AC) and gel consistency (GC) of head rice. Three mapping methods employed were composite interval mapping in QTLMapper 2.0 software based on mixed linear model (MCIM), inclusive composite interval mapping in QTL IciMapping 3.0 software based on stepwise regression linear model (ICIM) and multiple interval mapping with regression forward selection in Windows QTL Cartographer 2.5 based on multiple regression analysis (MIMR). Results showed that five QTLs with additive effect (A-QTLs) were detected by all the three methods simultaneously, two by two methods simultaneously, and 23 by only one method. Five A-QTLs were detected by MCIM, nine by ICIM and 28 by MIMR. The contribution rates of single A-QTL ranged from 0.89% to 38.07%. All the QTLs with epistatic effect (E-QTLs) detected by MIMR were not detected by the other two methods. Fourteen pairs of E-QTLs were detected by both MCIM and ICIM, and 142 pairs of E-QTLs were detected by only one method. Twenty-five pairs of E-QTLs were detected by MCIM, 141 pairs by ICIM and four pairs by MIMR. The contribution rates of single pair of E-QTL were from 2.60% to 23.78%. In the Xiu-Bao RIL population, epistatic effect played a major role in the variation of GL and CD, and additive effect was the dominant in the variation of LWR, while both epistatic effect and additive effect had equal importance in the variation of CGR, AC, GT and GC. QTLs detected by two or more methods simultaneously were highly reliable, and could be applied to improve the quality traits in japonica hybrid rice. 相似文献
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以粳粳交组合秀水79/C堡衍生的254个重组自交系为材料,利用基于混合线性模型的QTLMapper 2.0软件的复合区间作图法(MCIM)、基于逐步回归线性模型的QTL IciMapping 3.0软件的完备复合区间作图法(ICIM)和基于多元回归分析的Windows QTL Cartographer 2.5软件的多区间作图回归前进选择法(MIMR)等3种定位方法,对整精米的粒长、长宽比、垩白粒率、垩白度、直链淀粉含量、糊化温度和胶稠度等7个米质性状进行了QTL分析。结果表明,3种方法同时检测到的具有加性效应的QTL (A QTL)有5个,2种方法同时检测到的A QTL有2个,仅能在1种方法中检测到的A QTL有23个。MCIM、ICIM和MIMR检测到的A QTL个数分别为5、9和28,单个A QTL贡献率为0.89%~38.07%。MIMR检测到的具有上位性效应的QTL (E QTL)在另2种方法中都未被检测到。MCIM 和ICIM同时检测到的E QTL有14对,仅能在1种方法中检测到的E QTL有142对。MCIM、ICIM和MIMR检测到的E QTL对数分别为25、141和4,单对E QTL贡献率为2.60%~23.78%。在秀堡RIL群体中,粒长和垩白度的变异以上位性效应为主,长宽比则以加性效应为主,而垩白粒率、直链淀粉含量、糊化温度和胶稠度为加性效应和上位性效应同等重要。两种及以上方法同时检测到的QTL可靠性高,可用于改良杂交粳稻米质。 相似文献
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利用重组自交系群体定位玉米生育期相关性状QTL 总被引:1,自引:0,他引:1
利用玉米自交系80007和80044为亲本,衍生包含355个家系的重组自交系(RIL)F9群体,采用SSR标记构建包括219个标记的连锁图谱,图谱总长度2 133.5 cM,标记间平均距离9.7 cM。采用完备区间作图法对控制玉米抽雄期、散粉期、吐丝期以及散粉至吐丝间隔期的4个生育期相关性状进行QTL分析。结果表明,共检测到60个QTL,其中抽雄期检测到20个QTL,吐丝期检测到15个QTL,散粉期检测到20个QTL,散粉至吐丝间隔期检测到5个QTL。共有7个QTL对表型变异的解释率超过了10%,表现为主效QTL效应,分布在第3、4和第9染色体。有11个QTL在不同环境中能重复检测出,是受环境影响较小、为较稳定的QTL。分析发现,生育期相关性状的QTL在染色体上有"成簇"分布的现象,并且贡献率较大的QTL控制着多个相关性状。结果表明,bin3.04-3.05、bin3.09、bin7.03和bin9.02-9.03是生育期相关性状QTL的密集区域,这些区域存在对生育期相关性状重要作用的位点。 相似文献
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玉米主要营养品质性状的QTL定位 总被引:1,自引:0,他引:1
以LX00-6×E28的278个F2:3家系为作图群体,通过SSR标记利用MAPMAKER/EXP3.0和Mapdraw 2.0构建遗传连锁图谱.该连锁图覆盖玉米基因组1 508.1 cM,包含124个标记,相邻两标记的平均距离为12.2 cM.利用QTLMaper2.5软件,结合主要品质性状的检测结果,运用复合区间作图法,以LOD=2.0对玉米主要品质性状进行全基因组QTLs扫描,检测到两个与淀粉含量相关的QTL位点,分别位于第1、8条染色体上,表现为部分显性效应和加性效应,并在第1条染色体上检测到1个与油分含量相关的位点,表现为加性效应. 相似文献
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水稻顶部三叶与穗重的关系及其QTL分析 总被引:17,自引:2,他引:17
摘要: 对水稻汕优63重组自交系群体顶部3张叶片的长、宽、重和单穗重等10个性状进行了相关分析和QTL定位。穗重与9个叶片性状存在极显著的正相关,其中与倒2叶重的相关系数最大,剑叶重次之。所有性状在重组自交系群体中均存在双向超亲分离,接近正态分布。共检测到44个主效QTL和43对双位点互作影响上述10个性状。主效QTL分布于水稻的除第8染色体外的其余11条染色体上,贡献率介于3.19%~26.23%;互作分布于水稻的12条染色体上,贡献率变幅为2.03%~8.93%。第2染色体的R2510-RM211标记区间同时检测到控制单穗重和倒2叶重的QTL,该QTL对超级稻株型育种具有应用价值。 相似文献
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《Field Crops Research》2006,96(1):106-112
Improvement of rice (Oryza sativa L.) plant type is a major breeding objective. This study aimed to precisely localize and characterize key genomic regions for plant type using near-isogenic individuals. Selfing of partially heterozygous F5 recombinant inbred (RI) individuals [parental varieties Milyang 23 (M23) and Akihikari (AK)] developed heterogeneous inbred families (HIFs) composed of 108 and 93 F7 progenies, which segregated at molecular marker loci on the long arms of chromosomes 4 and 6, respectively. The progeny lines (F8) were evaluated for traits composing plant type in Los Baños, Philippines, to locate quantitative trait loci (QTLs) using interval mapping and to evaluate the effects of the QTL region by phenotypic comparison between the genotypes. QTLs for the traits were detected in 17 cM across XNpb12 on chromosome 6. The M23 homozygote for the QTL region resulted in a >7% increase and decrease in plant length and tiller number at heading, respectively, relative to the AK homozygote. Consequently, culm length (CL) and traits determining flag-leaf and panicle sizes increased 5–56% by the M23 homozygote, together with a 15% decrease in panicle number. For a QTL region detected in 6 cM across XNpb235 on chromosome 4, the AK homozygote had similar effects on these traits, except CL. The directions and magnitudes of their effects agreed with those previously estimated in the RI line population, thus increasing confidence in primary QTL analyses for plant type. Analyzing the HIFs validated and characterized the two QTL regions greatly involved in determining varietal plant type from an early growth stage to maturity, providing information useful for empirical and marker-assisted breeding towards rice improvement. 相似文献
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利用F2:3群体对玉米花期相关性状的QTL分析 总被引:2,自引:2,他引:0
以玉米自交系四287与四144为亲本的188个F2:3家系为材料, 考察玉米花期相关性状, 并利用该群体的分子遗传连锁图谱进行QTL分析。结果表明, 共检测到11个与玉米花期相关的QTL, 包括与抽雄期相关的2个QTL, 位于第3和第8染色体上, 贡献率分别为21.9%和13.6%;与散粉期相关的3个QTL, 位于第1、3和第8染色体上, 贡献率分别为14.8%、21.0%和18.5%;与吐丝期相关的2个QTL, 位于第3和第8染色体上, 贡献率分别为16.5%和20.1%;与散粉-吐丝间隔期相关的4个QTL, 位于第1、6、7和第8染色体上, 贡献率分别为23.5%、25.3%、28.9%和20.9%。这些QTL的基因效应以部分显性和显性为主。 相似文献
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Kernel size and shape in wheat are important because of their relationship with yield and milling quality. This paper reports QTL analyses of kernel morphology in two hexaploid wheat mapping populations, grown in NY and CA. Kernel morphology was evaluated through a new and improved method, combining measurements from two orthogonal pictures. Single marker regression showed that several genomic positions, scattered through the genome, were related to kernel size and shape, in both populations. The direction of allele effects was consistent between environments, although the LOD scores varied considerably. Composite interval mapping revealed QTLs on all seven homoeologous groups, considering both populations. For the QTLs detected through this method, the signal and magnitude of additive effects were similar between environments, indicating small QTL × environment interaction. In the population W7984 × Opata 85, the strongest signal was detected on the chromosome 5B, for kernel length. In the population AC Reed × Grandin, the most important QTLs were detected on chromosome 2D, affecting the lateral dimensions of the kernel. This study agreed with previous reports that the genetic control of kernel length and width are largely independent. Additionally, it was shown that QTLs detected on different mapping populations, with identical evaluation methods, can be very distinct. 相似文献
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对元江普通野生稻(Oryza rufipogon Griff.)(简称元江普野)荷花塘3号为供体、籼稻(O.sativassp.indica)恢复系蜀恢527为轮回亲本构建的种间近等基因系群体进行数量性状位点(QTL)分析,在近等基因导入系YJ10-03-01中鉴定了一个抽穗扬花期耐热QTL。利用均匀分布在水稻12条染色体上的360个SSR标记检测近等基因系YJ10-03-01和籼稻恢复系蜀恢527,共获得9个多态性标记。单标记分析表明第5染色体短臂上的多态性标记与抽穗扬花期耐热性极显著相关。进一步在人工气候室模拟高温条件下处理YJ10-03-01与蜀恢527杂交得到F2分离群体(1027个单株)并进行SSR标记分析,以水稻结实率为耐热指标,利用复合区间作图方法在第5染色体短臂上检测到一个抽穗扬花期耐热性QTL,暂命名为qHTH5。该QTL在F2及F3世代分别解释8.6%和19.4%的表型变异。在F3世代,继续利用目标区间标记RM7320和RM7444之间的SSR标记鉴定纯合重组体,利用置换作图法将QTL定位在约304.2kb之内(RM592-RM17921)。 相似文献
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不同种植密度条件下玉米茎秆穿刺强度QTL分析 总被引:2,自引:1,他引:1
以四287与四144为亲本组配的188个F2:3家系为材料,分别于2013、2014年在2个种植密度下调查茎秆穿刺强度,采用复合区间作图法进行QTL分析,研究玉米茎秆穿刺强度在不同种植密度下的遗传机制。结果表明,在2个种植密度下,共检测到13个与茎秆穿刺强度相关的QTL,分布于玉米的第1、2、3、4、6、7、8、9染色体上,单个QTL可解释3.8%~11.8%的表型变异,位于第6染色体检测到的QTL在所有环境中均能稳定表达。 相似文献
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三个小麦赤霉病抗源的抗性QTL定位 总被引:8,自引:1,他引:7
为寻找小麦赤霉病抗性基因及可用于分子标记辅助育种的抗性连锁标记.对中国的三个小麦赤霉病抗源苏麦3号、望水白和宁894037进行了抗性QTL的定位研究。SSR、AFLP分析与QTL分析结果表明,尽管三个抗源的来源和遗传背景并不同,但均在3B染色体短臂上发现抗性主效QTL,不同遗传群体所获得的QTL位点所处的染色体区段略有差异,位于QTL两翼的SSR标记也有所不同。苏麦3号的赤霉病抗性主效QTL位于3B染色体上的标记区间Xgwm533~Xgwm493内;宁894037的抗性位点分布于3B和6B染色体上。分别定位于标记Xgwm493~Xbarcl33和Xgwm644-Xgwm518之间;望水白的抗性主效QTL也位于3B染色体上.定位于标记Xgwm493~Xbarc147之间。微效QTL由于遗传群体的不同,分别住于1B、3B和2A染色体上。研究还表明,寻找抗性QTL在3B染色体以外的新抗源十分必要。 相似文献
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不同遗传背景下水稻剑叶形态性状的QTL分析 总被引:7,自引:2,他引:7
以典型的籼粳交窄叶青8号/京系17的加倍单倍体(DH)群体和籼籼交珍汕97/明恢63的重组自交系(RIL)群体为材料,并应用这两种群体已构建的分子图谱,对剑叶形态性状中的叶面积、周长、叶长、叶宽、长宽比等进行了QTL分析。在这两个群体中,剑叶的这些形态性状均呈连续性分布,存在一定数量的超亲分离。在DH群体中, 4个剑叶性状共检测到8个QTL,分布在4条染色体上,其中第4、8染色体上各有1个QTL同时影响了叶长、 叶周长和面积。 在RIL群体中,5个剑叶性状共检测到16个QTL,分布在5条染色体上。其中第1染色体上有1个QTL同时影响叶长、叶周长、长宽比3个性状,第6染色体上有2个影响叶面积的QTL同时也影响叶宽,还有1个QTL同时影响叶长、叶周长2个性状,第11染色体上有1个QTL同时影响叶长、叶周长、叶面积3个性状。研究表明,不同群体的QTL初级定位结果存在较大差异,影响叶片相关性状的一些QTL位于同一染色体的相同或者相邻区域上。 相似文献
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【目的】发掘与产量相关的穗粒性状QTL对进一步克隆和利用高产基因具有重要意义。【方法】以超级粳稻龙稻5号和典型高产籼稻中优早8号杂交衍生的重组自交系群体为试材,在4种环境下对穗部性状进行比较和QTL分析。【结果】共检测到63个穗部性状QTL,分布于除第9染色体外的11条染色体上。在4个环境下分别检测到27、27、18和35个QTL。其中,16个QTL能在2个环境下被检测到,12个在3个以上环境下稳定表达,分别占QTL总数的25.40%和19.05%;第1、3、4和5染色体的多效QTL簇能在不同环境下稳定表达,对穗部性状具有明显的调控作用。【结论】第3染色体STS3.3-STS3.6区间的qSNP3、第4染色体RM5688-RM1359区间的qSNP4.1是2个新的稳定表达的多效性QTL簇。此外,上位性效应是调控穗部性状的重要组分。 相似文献
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粒形是影响小麦籽粒产量和品质的重要参数,是由多基因控制的复杂数量性状。为发掘控制小麦粒形相关的真实主效数量性状位点(quantitative trait loci, QTL),本研究利用BioMercator 4.2软件,以小麦高密度分子标记遗传图谱为参考图谱,对来自不同遗传作图群体的113个控制小麦粒长的QTL和86个控制粒宽的QTL进行图谱整合、映射以及QTL元分析。通过建立QTL一致性图谱,获得18个控制小麦粒长和8个控制粒宽的一致性QTL(meta quantitative trait loci, MQTL)位点,置信区间最小可达到0.57 cM,主要分布在2B、2D、3A、3B、4B、5A、5B和7D染色体上。在5A染色体Xgwm293~Xgwm304和Xgpw2120~Xgpw2273a标记区间内,预测到7个与小麦粒长和粒宽相关的候选基因。本研究为小麦粒形QTL精细定位以及分子标记辅助选择育种提供理论依据。 相似文献
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《Journal of Cereal Science》1999,29(2):153-160
Starch accumulates in barley (Hordeum vulgareL.) endosperm in large (type A) and small (type B) granules. The sizes, shapes and relative proportions of A and B granules may affect the quality of barley malt for brewing. The objective of this study was to use genetic markers to map quantitative trait loci (QTL) affecting starch granule traits in a cross between a malting barley cultivar, Morex, and a feed barley cultivar, Steptoe. Data on starch granule dimensions were obtained using digital image analysis. With simple interval mapping, a region of chromosome 2 (2H) was found to contain QTL affecting the overall mean granule volume, the proportion of A granules, the mean volume of A granules, the mean maximum diameter of A granules and the mean F-shape of B granules. This region also affected days to heading and plant height, but contained no QTL for grain or malt quality traits. With composite interval mapping, QTL affecting starch granule traits were detected in two additional regions, one on chromosome 4 (4H) affecting the mean F-shape of B granules and one on chromosome 7 (5H) affecting the mean maximum diameter of A granules. 相似文献
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水稻第6染色体短臂株高及产量性状QTL的分解 总被引:1,自引:1,他引:0
针对第6染色体短臂上一个对产量性状遗传具有重要作用的区间RM587-RM19715,从珍汕97B/密阳46重组自交系群体中筛选到1个剩余杂合体,自交衍生获得一个由195个个体组成的F2群体,检测控制株高和产量性状的QTL。经分析,在目标区间的上部和下部分别检测到1个QTL簇,分别对除单株穗数以外的产量性状因子具显著作用,单个QTL对群体性状表型变异的贡献率为5.0%~55.5%。将第6染色体上的产量性状QTL分解到更小的区间中,为产量性状QTL的精细定位和克隆打下了基础。 相似文献
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D. A. Bisognin N. C. Manrique-Carpintero D. S. Douches 《American Journal of Potato Research》2018,95(4):374-382
Tuber dormancy and sprouting are important traits in commercial potato varieties. Since wild Solanum species can contribute new alleles in breeding, we combined Solanum chacoense and S. berthaultii species in a mapping population to develop a linkage map with genome wide markers and identify quantitative trait loci (QTL) for tuber dormancy and sprouting. There was genetic variation for the evaluated traits and high correlation (0.80) between dormancy and apical dominance release. QTL for tuber dormancy and sprouting traits were mapped to the same or similar positions on chromosomes 2, 3, 5, and 7, explaining from 9.5% to 16.3% of the phenotypic variance. We identified candidate genes associated with all major phytohormone regulation in close linkage with the QTL. The QTL on chromosomes 2, 3 and 7 are good candidates for marker assisted breeding, since they were associated with dormancy and apical dominance release and should be useful to delay or accelerate tuber sprouting. 相似文献
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小麦籽粒形态及千粒重性状的QTL初步定位 总被引:2,自引:1,他引:1
为研究小麦籽粒形态及千粒重性状的QTL,以普通小麦6044和01-35为杂交组合构建的F8重组自交系(RIL)群体作为试验材料,在山东泰安(山东农业大学试验站)和莱阳(青岛农业大学试验站)两个环境下进行两年田间试验,利用Mapmaker/version 3.0和WinQTLCart软件通过复合区间作图法进行QTL初步定位,在两年两个环境下共检测到12个相关QTL位点,其中关于粒长的2个QTL分别位于2A和2B染色体上,可解释表型变异的25%和12%;4个粒厚QTL位于2A和6A染色体上,可解释表型变异的7%~10%;6个千粒重相关QTL位于染色体2A、4A和6A连锁群上,可解释表型变异的6%~25%;而粒宽QTL在两个地点上都没有检测到。其中相关性高的性状间有一些共同的QTL,表现出一因多效或紧密连锁效应。 相似文献