共查询到20条相似文献,搜索用时 15 毫秒
1.
M. Shamim Hasan ZAHID Sharda Prasad AWASTHI Atsushi HINENOYA Shinji YAMASAKI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(5):535-540
To search natural compounds having inhibitory effect on bacterial growth is important,
particularly in view of growing multidrug resistant (MDR) strains of bacterial pathogens.
Like other bacterial pathogens, MDR Vibrio cholerae, the causative agent
of diarrheal disease cholera, is becoming a great concern. As an approach of searching new
antimicrobial agents, here, we show that anethole, a well-studied natural component of
sweet fennel and star anise seeds, could potentially inhibit the growth of MDR O1 El Tor
biotype, the ongoing 7th cholera pandemic variant strains of toxigenic V.
cholerae. The minimum inhibitory concentration (MIC) of anethole against
diverse O1 El Tor biotype strains is evaluated as 200
µg/ml. Moreover, the effect of anethole is
bactericidal and exerts rapid-killing action on V.
cholerae cells. This study is the first report which demonstrates
that anethole, purified from natural compound, is a potent inhibitor of growth of
toxigenic V. cholerae. Our data suggest that anethole could be a
potential antimicrobial drug candidate, particularly against MDR V.
cholerae mediated infections. 相似文献
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Arunee THANASARASAKULPONG Pichayanut POOLPERM Pallop TANKAEW Takuo SAWADA Nattawooti STHITMATEE 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(3):321-326
Recombinant outer membrane protein H (rOmpH) is a potential fowl cholera vaccine
candidate. The present study was aimed at developing rOmpH formulations for intranasal
administration. The rOmpH was purified and formulated with either Escherichia
coli enterotoxin B (LTB) or CpG oligodeoxynucleotides (ODN) as an adjuvant.
Antibody responses in chickens intranasally immunized with rOmpH in combination with 2
different adjuvants were significantly increased (P<0.05) post
immunization. Chicken survival rates showed that rOmpH formulated with ODN and LTB
elicited 90% and 70% protection, respectively. Our findings indicated that rOmpH
formulated with ODN elicited protection better than that formulated with LTB. Therefore,
the vaccines formulations in the present study can be considered new intranasal vaccine
formulations for fowl cholera in chickens. 相似文献
4.
James A. Leigh Sharon A. Egan Philip N. Ward Terence R. Field Tracey J. Coffey 《Veterinary research》2010,41(5)
Streptococcus uberis, strain 0140J, contains a single copy sortase A (srtA), encoding a transamidase capable of covalently anchoring specific proteins to peptidoglycan. Unlike the wild-type, an isogenic mutant carrying an inactivating ISS1 insertion within srtA was only able to infect the bovine mammary gland in a transient fashion. For the first 24 h post challenge, the srtA mutant colonised at a similar rate and number to the wild type strain, but unlike the wild type did not subsequently colonise in higher numbers. Similar levels of host cell infiltration were detected in response to infection with both strains, but only in those mammary quarters infected with the wild type strain were clinical signs of disease evident. Mutants that failed to express individual sortase substrate proteins (sub0135, sub0145, sub0207, sub0241, sub0826, sub0888, sub1095, sub1154, sub1370, and sub1730) were isolated and their virulence determined in the same challenge model. This revealed that mutants lacking sub0145, sub1095 and sub1154 were attenuated in cattle. These data demonstrate that a number of sortase anchored proteins each play a distinct, non-redundant and important role in pathogenesis of S. uberis infection within the lactating bovine mammary gland. 相似文献
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Kiku Matsuda Atul A. Chaudhari Sam Woong Kim Kyeong Min Lee John Hwa Lee 《Veterinary research》2010,41(5)
To construct a novel live vaccine candidate for fowl typhoid (FT) caused by Salmonella Gallinarum (SG), the lon and cpxR genes that are related to host-pathogen interaction were deleted from a wild type SG using the allelic exchange method. The mutants were grown normally, as was the wild type. The biochemical properties of the mutants remained very similar to those of the wild-type, while JOL914 (Δlon) and JOL916 (ΔlonΔcpxR) were mucoid. Extracellular polysaccharide increased 30.6-, 1.3-, and 46.2-fold in JOL914, JOL915 (ΔcpxR), and JOL916, respectively. Dot-blot analysis demonstrated significant increases of FimA expression at 6.77-, 2.33-, and 3.90-fold for JOL914, JOL915, and JOL916, respectively. Internalizations of JOL914, JOL915, and JOL916, in chicken abdominal macrophages, were increased at 4.65-, 0.50-, and 2.72-fold, respectively. Virulences of JOL914, JOL915 and JOL916, analyzed by LD50 using 1-week-old chickens, were attenuated approximately at 101-, 101-, and > 103-fold, respectively. The oral inoculations of 2 × 107 cfu of the wild type, JOL914, JOL915 and JOL916 caused 55.6, 16.7, 22.2, and 0.0% mortality, respectively. Significantly moderate gross lesions of the liver and spleen were observed in the JOL916 group compared to the other groups. An induced immune response and significant peripheral mononuclear proliferation reaction were observed in the JOL916 group. At the protection against the wild type challenge, JOL916 offered 100% protection. Thus, the results of this study suggest that JOL916 among the mutants studied represented the safest and most effective live vaccine candidate against FT. 相似文献
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Toru TACHIBANA Ken Takeshi KUSAKABE Sayuri OSAKI Takeshi KURAISHI Shosaku HATTORI Midori YOSHIZAWA Chieko KAI Yasuo KISO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(1):161-165
The New World monkey Aotus spp. (night monkeys) are expected
for use of valuable experimental animal with the close species of Saimiri
spp. (squirrel monkeys). Saimiri is known to show spontaneous
hypercortisolemia, although few reports in Aotus. We compared basic
states of blood steroid hormones and histological structure of the adrenal glands in two
monkeys. Serum cortisol and ACTH levels were statistically lower in Aotus
than Saimiri. Conversely, Aotus adrenocortical area
showed significant enlargement, especially at the zona fasciculata. Electron microscopic
observation at Aotus fasciculata cells revealed notable accumulation of
large lipid droplets and irregular shapes of the mitochondrial cristae. These results
suggest potential differences in cellular activities for steroidogenesis between
Aotus and Saimiri and experimental usefulness in
adrenocortical physiology and pathological models. 相似文献
7.
Tomoaki Tochitani Izumi Matsumoto Kohei Hoshino Kaoru Toyosawa Mami Kouchi Takatoshi Koujitani Juki Kimura Hitoshi Funabashi 《Journal of toxicologic pathology》2013,26(2):187-191
The common marmoset (Callithrix
jacchus) is now widely used in various
research fields, including toxicology. However,
information about the background pathology of this
species is scarce. Here, we report a case of
rhabdomyosarcoma that spontaneously occurred in a
common marmoset. A 44-month-old male common
marmoset was euthanized due to bilateral hind limb
paralysis. At necropsy, a 2×2×5-cm intramuscular
mass was observed in the lower right back.
Histologically, the mass was mainly composed of
interlacing bundles of spindle-shaped tumor cells.
Immunohistochemically, the tumor cells were
positive for myogenin, desmin, vimentin and
alpha-smooth muscle actin. Ultrastructurally, the
tumor cells contained bundles of myofilaments with
Z-band-like structures. Thus, the tumor was
diagnosed as a rhabdomyosarcoma. To our knowledge,
this is the first report of spontaneous
rhabdomyosarcoma that was definitely diagnosed in
the common marmoset. 相似文献
8.
Irfan Ahmad MIR Bablu KUMAR Anil TAKU Farah FARIDI Mohd. Altaf BHAT Naseer Ahmad BABA Tahir MAQBOOL 《Journal of Equine Science》2013,24(3):53-55
Present study was undertaken to study the prevalence of β-haemolytic streptococci in
equine of northern temperate region of Jammu and Kashmir, India. One hundred and forty one
samples were collected in duplicate from nasopharyngeal tract of diseased (53) and
apparently healthy equine (88) for isolation and direct PCR. A total of 77 isolates of
streptococci were recovered from 141 samples with an overall prevalence rate of 54.60%.
Out of these 77 isolates, 52 were from diseased and 25 from apparently healthy animals. Of
the 77 isolates, 4 were identified as Streptococcus equi subsp.
equi, 56 as S. equi subsp.
zooepidemicus and 17 as S. dysgalactiae subsp.
equisimilis. Thus the overall prevalence of S. equi subsp.
equi, S. equi subsp. zooepidemicus
and S. dysgalactiae subsp. equisimilis was 2.83, 39.71
and 12.05% respectively. The sensitivity of the PCR for the detection of S.
equi species was found higher when attempted from direct swab samples. 相似文献
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Mohamed M’BAYE Guohua HUA Hamid Ali KHAN Liguo YANG 《The Journal of reproduction and development》2015,61(5):391-397
Inhibins are members of the TGFβ superfamily and act as suppressors
of follicle stimulating hormone (FSH) secretion from pituitary glands
via a negative feedback mechanism to regulate folliculogenesis. In
this study, the INHBB gene was knocked down by three
RNAi-Ready pSIREN-RetroQ-ZsGreen vector- mediated recombinant plasmids
to explore the effects of INHBB silencing on
granulosa cell (GC) cell cycle, apoptosis and steroid production
in vitro. Quantitative real-time polymerase chain
reaction, Western blot, flow cytometry and ELISA were performed to
evaluate the role of INHBB in the mouse GC cell
cycle, apoptosis and steroid production in vitro. The
results showed that the relative mRNA and protein expression of
INHBB in mouse GCs can be significantly reduced by
RNAi with pshRNA-B1, pshRNA-B2 and pshRNA-B3 plasmids, with pshRNA-B3
having the best knockdown efficiency. Downregulation of the expression
of INHBB significantly arrests cells in the G1 phase
of the cell cycle and increases the apoptosis rate in GCs. This was
further confirmed by downregulation of the protein expressions of
Cyclin D1, Cyclin E and Bcl2, while the protein expression of Bax was
upregulated. In addition, specific downregulation of
INHBB markedly decreased the concentration of
estradiol and progesterone, which was further validated by the
decrease in the mRNA levels of CYP19A1and
CYP11A1. These findings suggest that inhibin βB is
important in the regulation of apoptosis and cell cycle progression in
granulosa cells. Furthermore, the inhibin βB subunit has a role in the
regulation of steroid hormone biosynthesis. Evidence is accumulating
to support the concept that inhibin βB is physiologically essential
for early folliculogenesis in the mouse. 相似文献
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Shin-ichi NAKAMURA Hideki HAYASHIDANI Yukari SOTOHIRA Yumi UNE 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(2):297-299
Two captive Keel-billed toucans and a Chestnut-mandibled toucan in another zoological garden died suddenlywithout any pre-existing symptoms, and three months later, a Japanese squirrel died of diarrhea. All theseanimals showed necrotic enteritis and multifocal necrosis in the liver and spleen with Gram negative bacilli.The bacilli showed strong positive immunolabeling for Yersinia pseudotuberculosis O4 in theKeel-billed toucans, Y. pseudotuberculosis O2 in the Chestnut-mandibled toucan and Y.pseudotuberculosis O1 in the Japanese squirrel, while Y. pseudotuberculosis 4b, 2band 1b were respectively isolated from the lesions. To our knowledge, this might be the first reported case offatal yersiniosis in a Japanese squirrel in the world as well as in toucans in Japan. 相似文献
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Kohei Matsushita Aki Kijima Yuji Ishii Shinji Takasu Meilan Jin Ken Kuroda Hiroaki Kawaguchi Noriaki Miyoshi Takehiko Nohmi Kumiko Ogawa Takashi Umemura 《Journal of toxicologic pathology》2013,26(1):19-27
In this study, the potential for development of an animal model (GPG46) capable of rapidly detecting chemical carcinogenicity and the underlying mechanisms of action were examined in gpt delta rats using a reporter gene assay to detect mutations and a medium-term rat liver bioassay to detect tumor promotion. The tentative protocol for the GPG46 model was developed based on the results of dose-response exposure to diethylnitrosamine (DEN) and treatment with phenobarbital over time following DEN administration. Briefly, gpt delta rats were exposed to various chemicals for 4 weeks, followed by a partial hepatectomy (PH) to collect samples for an in vivo mutation assay. The mutant frequencies (MFs) of the reporter genes were examined as an indication of tumor initiation. A single intraperitoneal (ip) injection of 10 mg/kg DEN was administered to rats 18 h after the PH to initiate hepatocytes. Tumor-promoting activity was evaluated based on the development of glutathione S-transferase placental form (GST-P)-positive foci at week 10. The genotoxic carcinogens 2-acetylaminofluorene (2-AAF), 2-amino-3-methylimidazo [4,5-f] quinolone (IQ) and safrole (SF), the non-genotoxic carcinogens piperonyl butoxide (PBO) and phenytoin (PHE), the non-carcinogen acetaminophen (APAP) and the genotoxic non-hepatocarcinogen aristolochic acid (AA) were tested to validate the GPG46 model. The validation results indicate that the GPG46 model could be a powerful tool in understanding chemical carcinogenesis and provide valuable information regarding human risk hazards. 相似文献
20.
Maiko OHTORI Mikiko AOKI Tadashi ITAGAKI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(1):105-107
This study was designed to clarify the differences in the internal transcribed
spacer (ITS) 1 and 5.8S nucleotide sequences of Moniezia expansa,
M. benedeni and M. monardi isolated from ruminants in
Japan and to determine their phylogenetic relationships. A 98% similarity in the 5.8S
sequences was observed among the 3 Moniezia species, whereas many
nucleotide indels and substitutions were observed in the ITS1 sequences among the three
Moniezia species. These results suggest that the ITS1 region could
serve as a potential marker for discriminating the 3 Moniezia species. In
the phylogenetic tree based on the ITS1 sequences, M. monardi and
M. benedeni showed genetically closer relationship to each other than
to M. expansa. 相似文献