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A resistant phenotype similar to that conferred in wheat by the complementary genes Lr27 + Lr31 was produced in the progeny of intercrosses of cultivars carrying Lr27 and a line possessing Lr12 . This confirms that Lr12 is either completely linked with Lr31 or is the same gene. On the basis of these findings and that Lr31 is located on chromosome 4BS, it is concluded that Lr12 must also be located on 4BS. Adult-plant genetic tests confirm that the Australian wheat cultivar Timgalen carries Lr12 , and stocks with Lr12 alone were established from this cultivar. 相似文献
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Close genetic linkage of genes conferring adult-plant resistance to leaf rust and stripe rust in wheat 总被引:11,自引:1,他引:10
R. A. MCINTOSH 《Plant pathology》1992,41(5):523-527
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Molecular marker mapping of leaf rust resistance gene lr46 and its association with stripe rust resistance gene yr29 in wheat 总被引:1,自引:0,他引:1
ABSTRACT Leaf and stripe rusts, caused by Puccinia triticina and P. striiformis, respectively, are globally important fungal diseases of wheat that cause significant annual yield losses. A gene that confers slow rusting resistance to leaf rust, designated as Lr46, has recently been located on wheat chromosome 1B. The objectives of our study were to establish the precise genomic location of gene Lr46 using molecular approaches and to determine if there was an association of this locus with adult plant resistance to stripe rust. A population of 146 F(5) and F(6) lines produced from the cross of susceptible 'Avocet S' with resistant 'Pavon 76' was developed and classified for leaf rust and stripe rust severity for three seasons. Using patterns of segregation for the two diseases, we estimated that at least two genes with additive effects conferred resistance to leaf rust and three to four genes conferred resistance to stripe rust. Bulked segregant analysis and linkage mapping using amplified fragment length polymorphisms with the 'Avocet' x 'Pavon 76' population, F(3) progeny lines of a single chromosome recombinant line population from the cross 'Lalbahadur' x 'Lalbahadur (Pavon 1B)', and the International Triticeae Mapping Initiative population established the genomic location of Lr46 at the distal end of the long arm of wheat chromosome 1B. A gene that is closely linked to Lr46 and confers moderate levels of adult plant resistance to stripe rust is identified and designated as Yr29. 相似文献
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Histological studies of the expression of the Lr9, Lr20 and Lr28 alleles for resistance to leaf rust in wheat 总被引:4,自引:1,他引:3
The development of the leaf rust fungus ( Puccinia recondita f.sp. tritici ) in a susceptible cultivar and three other cultivars possessing the Lr9, Lr20 and Lr28 alleles for resistance was studied by light and fluorescence microscopy. Formation of the substomatal vesicle, intercellular hypha and the first haustorial mother cell was unaffected by resistance. Lr9 and Lr28 expression was rapid, first seen as early initiation of hyphal branching at 16 h after inoculation, then reduced haustorial diameters at 19 h. Limited host cell necrosis was seen immediately afterwards. Elongation of intercellular hyphae was reduced between 20 and 24 h, and virtually ceased by about 30 h. Numbers of infection sites with a second haustorial mother cell were briefly higher at 24 h. Reduced hyphal branching and haustorial mother cell numbers were seen at 20–24 h and 36 h respectively. Lr20 expression was not seen until 36 h when reduced hyphal branching was observed, accompanied by extensive host cell necrosis. Reduced haustorial mother cell numbers were detected at 48 h. Findings suggested a secondary role for host cell necrosis in the expression of the Lr9 and Lr28 alleles. Host necrosis may play a determinant role in Lr20- based expression. 相似文献
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[目的]建立简单、快速、有效的小麦抗叶锈基因复合PCR体系,从而提高分子标记辅助选择效率。[方法]以28个‘Thatcher’为背景的近等基因系和16个已知基因载体品系作为试材,测试了小麦抗叶锈病基因Lr9、Lr26、Lr19和Lr20的STS标记特异性,通过优化PCR反应体系和循环条件,构建了抗叶锈基因Lr9-Lr26和Lr19-Lr20的复合PCR检测体系。对116个小麦品种(系)所含有的抗叶锈病基因进行了分子检测。[结果]供试品种均不含有Lr9和Lr20,47个品种含有Lr26(基因频率为40.5%),‘中梁22’含有Lr19。经反复验证,Lr9-Lr26和Lr19-Lr20复合PCR技术检测结果可靠,且与上述单个分子标记检测结果一致。[结论]建立的Lr9-Lr26和Lr19-Lr20的复合PCR检测体系可以准确、稳定、高效地检测小麦抗叶锈基因Lr9、Lr26、Lr19和Lr20。 相似文献
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ABSTRACT Most barley cultivars are resistant to stripe rust of wheat that is caused by Puccinia striiformis f. sp. tritici. The barley cv. Steptoe is susceptible to all identified races of P. striiformis f. sp. hordei (PSH), the barley stripe rust pathogen, but is resistant to most P. striiformis f. sp. tritici races. To determine inheritance of the Steptoe resistance to P. striiformis f. sp. tritici, a cross was made between Steptoe and Russell, a barley cultivar susceptible to some P. striiformis f. sp. tritici races and all tested P. striiformis f. sp. hordei races. Seedlings of parents and F(1), BC(1), F(2), and F(3) progeny from the barley cross were tested with P. striiformis f. sp. tritici races PST-41 and PST-45 under controlled greenhouse conditions. Genetic analyses of infection type data showed that Steptoe had one dominant gene and one recessive gene (provisionally designated as RpstS1 and rpstS2, respectively) for resistance to races PST-41 and PST-45. Genomic DNA was extracted from the parents and 150 F(2) plants that were tested for rust reaction and grown for seed of F(3) lines. The infection type data and polymorphic markers identified using the resistance gene analog polymorphism (RGAP) technique were analyzed with the Mapmaker computer program to map the resistance genes. The dominant resistance gene in Steptoe for resistance to P. striiformis f. sp. tritici races was mapped on barley chromosome 4H using a linked microsatellite marker, HVM68. A linkage group for the dominant gene was constructed with 12 RGAP markers and the microsatellite marker. The results show that resistance in barley to the wheat stripe rust pathogen is qualitatively inherited. These genes might provide useful resistance against wheat stripe rust when introgressed into wheat from barley. 相似文献
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ABSTRACT Stripe rust is one of the most important diseases of wheat and barley worldwide. On wheat it is caused by Puccinia striiformis f. sp. tritici and on barley by P. striiformis f. sp. hordei Most wheat genotypes are resistant to P. striiformis f. sp. hordei and most barley genotypes are resistant to P. striiformis f. sp. tritici. To determine the genetics of resistance in wheat to P. striiformis f. sp. hordei, crosses were made between wheat genotypes Lemhi (resistant to P. striiformis f. sp. hordei) and PI 478214 (susceptible to P. striiformis f. sp. hordei). The greenhouse seedling test of 150 F(2) progeny from the Lemhi x PI 478214 cross, inoculated with race PSH-14 of P. striiformis f. sp. hordei, indicated that Lemhi has a dominant resistance gene. The single dominant gene was confirmed by testing seedlings of the F(1), BC(1) to the two parents, and 150 F(3) lines from the F(2) plants with the same race. The tests of the F(1), BC(1), and F(3) progeny with race PSH-48 of P. striiformis f. sp. hordei and PST-21 of P. striiformis f. sp. tritici also showed a dominant gene for resistance to these races. Cosegregation analyses of the F(3) data from the tests with the two races of P. striiformis f. sp. hordei and one race of P. striiformis f. sp. tritici suggested that the same gene conferred the resistance to both races of P. striiformis f. sp. hordei, and this gene was different but closely linked to Yr21, a previously reported gene in Lemhi conferring resistance to race PST-21 of P. striiformis f. sp. tritici. A linkage group consisting of 11 resistance gene analog polymorphism (RGAP) markers was established for the genes. The gene was confirmed to be on chromosome 1B by amplification of a set of nullitetrasomic Chinese Spring lines with an RGAP marker linked in repulsion with the resistance allele. The genetic information obtained from this study is useful in understanding interactions between inappropriate hosts and pathogens. The gene identified in Lemhi for resistance to P. striiformis f. sp. hordei should provide resistance to barley stripe rust when introgressed into barley cultivars. 相似文献
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C. A. Kamlofski E. Antonelli C. Bender M. Jaskelioff† C. H. Danna‡ R. Ugalde A. Acevedo 《Plant pathology》2007,56(1):46-54
A lesion-mimic mutant was obtained from a mutagenic treatment performed with ethyl methanesulfonate on the Argentine bread wheat ( Triticum aestivum ) cultivar Sinvalocho M.A. The HLP (hypersensitive-like phenotype) mutant exhibited tiny, discrete, white lesions in the absence of any pathogen, resembling the typical hypersensitive response (HR). The lesions only became evident once the fifth or sixth leaf emerged, and spread at random along the leaf blades and leaf sheaths of the developing plant, including tissues of the spike. Because the lesion-mimic mutant showed no lesions at the seedling stage, the phenotypes of both the mutant and its mother line were identical at this point. Histochemical studies showed that spontaneous hypersensitive-like lesions in the HLP mutant corresponded to cell death. In leaf-rust ( Puccinia triticina ) infection experiments performed at seedling and adult-plant stages, adult HLP plants showed enhanced resistance to leaf-rust attack compared with plants of Sinvalocho M.A. of comparable developmental stage, suggesting that the HLP mutation may confer increased resistance to the fungus. Because enhanced resistance coincided with the presence of spontaneous HR lesions, activation of HLP plant defence responses appeared to be tightly linked to this phenomenon. Final plant height and yield components in the lesion-mimic mutant did not differ from those of the mother line, indicating that the HLP mutation caused no detrimental pleiotropic effects that significantly affected agronomic performance. These data support the direct use of mutations in disease-resistance breeding. 相似文献
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Genetic architecture of adult plant resistance to leaf rust in a wheat association mapping panel
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Leaf rust caused by Puccina triticina is one of the most destructive fungal diseases of wheat (Triticum aestivum). Adult plant resistance (APR) is an effective strategy to achieve long‐term protection from the disease. In this study, findings are reported from a genome‐wide association study (GWAS) using a panel of 96 wheat cultivars genotyped with 874 Diversity Arrays Technology (DArT) markers and tested for adult leaf rust response in six field trials. A total of 13 quantitative trait loci (QTL) conferring APR to leaf rust were identified on chromosome arms 1BL, 1DS, 2AS, 2BL, 2DS, 3BS, 3BL, 4AL, 6BS (two), 7DS, 5BL/7BS and 6AL/6BS. Of these, seven QTLs mapped close to known resistance genes and QTLs, while the remaining six are novel and can be used as additional sources of resistance. Accessions with a greater number of combined QTLs for APR showed lower levels of disease severity, demonstrating additive and significant pyramiding effects. All QTLs had stable main effects and they did not exhibit a significant interaction with the experiments. These findings could help to achieve adequate levels of durable resistance through marker‐assisted selection and pyramiding resistance QTLs in local germplasm. 相似文献
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Aegilops sharonensis (Sharon goatgrass) is a wild relative of wheat and a rich source of genetic diversity for disease resistance. The objectives of this study were to determine the genetic basis of leaf rust, stem rust, and powdery mildew resistance in A. sharonensis and also the allelic relationships between genes controlling resistance to each disease. Progeny from crosses between resistant and susceptible accessions were evaluated for their disease reaction at the seedling and/or adult plant stage to determine the number and action of genes conferring resistance. Two different genes conferring resistance to leaf rust races THBJ and BBBB were identified in accessions 1644 and 603. For stem rust, the same single gene was found to confer resistance to race TTTT in accessions 1644 and 2229. Resistance to stem rust race TPMK was conferred by two genes in accessions 1644 and 603. A contingency test revealed no association between genes conferring resistance to leaf rust race THBJ and stem rust race TTTT or between genes conferring resistance to stem rust race TTTT and powdery mildew isolate UM06-01, indicating that the respective resistance genes are not linked. Three accessions (1644, 2229, and 1193) were found to carry a single gene for resistance to powdery mildew. Allelism tests revealed that the resistance gene in accession 1644 is different from the respective single genes present in either 2229 or 1193. The simple inheritance of leaf rust, stem rust, and powdery mildew resistance in A. sharonensis should simplify the transfer of resistance to wheat in wide crosses. 相似文献
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为明确青春221中抗叶锈病基因的有效性及其遗传机制,以青春221为父本,分别与感病品种铭贤169和Thatcher以及小麦抗叶锈病近等基因系Lr1、Lr3和Lr23的载体品系杂交,获得F1、F2和F3代群体后,分别在苗期和成株期进行抗病性测定。苗期,青春221对致病类型FGJ/QP、SHJ/GN和PHT/RP的抗病性分别由显性抗叶锈病基因Lr1、Lr3和Lr23控制,对致病类型BGD/HL的抗病性则由上述3对显性抗病基因独立或重叠作用控制,与Thatcher杂交后,青春221中的Lr23趋于隐性遗传并被Thatcher中的1对显性抑制基因抑制;成株期,青春221对优势致病类型PHT/RP和THT/TP混合菌种的抗病性由显性抗叶锈病基因Lr23控制,而Lr1和Lr3均不起作用。 相似文献
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以小麦优异抗源Sw92为父本,感病小麦品种铭贤169为母本,杂交获得F1、F2和BC1代群体。采用我国叶锈菌优势小种PHT对双亲及其杂交世代进行接种鉴定。结果表明,小麦抗源Sw92对叶锈菌小种PHT的抗性系由一对隐性基因所控制。采用简单重复序列(SSR)技术对Sw92携带的抗性基因进行分子标记,共筛选了371对SSR引物,获得2个引物(WMC494、WMC737)可在抗/感池和双亲中扩增出多态性DNA片段。遗传连锁分析结果表明,该抗病基因位于小麦6BS上,与WMC494、WMC737标记的遗传距离分别为3.4cM和15.0cM,不同于6BS上的已知抗叶锈基因Lr36和Lr53,暂命名为LrSw92。 相似文献
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The aim of this study was to investigate the potential diversity and pathogen‐specificity of sources of quantitative resistance to leaf rust caused by Puccinia triticina in French wheat germplasm. From a set of 86 genotypes displaying a range of quantitative resistance levels during field epidemics, eight wheat genotypes were selected and challenged in a greenhouse with three isolates of the pathogen, belonging to different pathotypes. Five components of resistance were assessed: infection efficiency, for which an original methodology was developed, latent period, lesion size, spore production per lesion, and spore production per unit of sporulating tissue. High diversity and variability for all these components were expressed in the host × pathotype combinations investigated; isolate‐specificity was found for all the components. The host genotypes displayed various resistance profiles, based on both the components affected and the isolate‐specificity of the interaction. Their usefulness as sources of quantitative resistance was assessed: line LD7 probably combines diversified mechanisms of resistance, being highly resistant for all the components, but displaying isolate‐specificity for all the components; cv. Apache did not show isolate specificity for any of the components, which could be related to the durability of its quantitative resistance in the field over more than 11 years. 相似文献
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X. Wang B. D. McCallum T. Fetch G. Bakkeren G. F. Marais B. J. Saville 《Plant pathology》2013,62(3):698-707
Thatcher near‐isogenic lines (NILs) of wheat carrying resistance gene Lr2a, Lr3, LrB or Lr9 were inoculated with Puccinia triticina races of virulence phenotype BBBD, MBDS, SBDG and FBDJ. Puccinia triticina infection structures were analysed under the fluorescence microscope over a course of 14 days after inoculation (dai). The relative proportion of P. triticina and wheat genomic DNA in infected leaves was estimated with a semiquantitative multiplex PCR analysis using P. triticina‐ and wheat‐specific primers. The occurrence of a hypersensitive response (HR), cellular lignification and callose deposition in inoculated plants was investigated microscopically. In interactions producing highly resistant infection type (IT) ‘0;’, a maximum of two haustorial mother cells per infection site were produced, and there was no increase in the proportion of P. triticina genomic DNA in infected leaves, indicating the absence of P. triticina growth. In comparison, sizes of P. triticina colonies increased gradually in interactions producing moderately resistant IT ‘1’ and ‘2’, with the highest proportion of P. triticina genomic DNA found in leaves sampled at 14 dai. In interactions producing susceptible IT ‘3–4’, the highest proportion of P. triticina genomic DNA was found in leaves sampled at 10 dai (45·5–51·5%). HR and cellular lignification were induced in interactions producing IT ‘0;’ and ‘1’ at 1 dai but they were not observed in interactions producing IT ‘2’ until 2 dai. No HR or cellular lignification were induced in interactions producing susceptible IT ‘3–4’. Furthermore, a strong deposition of callose was induced in Lr9 + BBBD and Lr9 + FBDJ (IT ‘0;’), whereas this defence response was not induced in resistant or susceptible interactions involving Lr2a, Lr3 or LrB, indicating that Lr9 mediated resistance was different from that conditioned by Lr2a, Lr3 or LrB. 相似文献
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ABSTRACT Alsen is a recently released spring wheat cultivar that has been widely grown in the United States because it has resistance to Fusarium head blight and leaf rust caused by Puccinia triticina. Norm is a high yielding wheat cultivar that has been very resistant to leaf rust since it was released. Alsen and Norm were genetically examined to determine the number and identity of the leaf rust resistance genes present in both wheats. The two cultivars were crossed with leaf rust susceptible cv. Thatcher and F(1) plants were backcrossed to Thatcher. Eighty one and seventy three BCF(1) of Thatcher times; Alsen and Thatcher x Norm respectively, were selfed to obtain BCF(2) families. The BCF(2) families were tested as seedlings with different isolates of P. triticina that differed for virulence to specific leaf rust resistance genes. The BCF(2) families that lacked seedling resistance were also tested as adult plants in greenhouse tests and in a field rust nursery plot. Segregation of BCF(2) families indicated that Alsen had seedling genes Lr2a, Lr10, and Lr23 and adult plant genes Lr13 and Lr34. Norm was determined to have seedling genes Lr1, Lr10, Lr16, and Lr23 and adult plant genes Lr13 and Lr34. The characterization of Lr23 in the segregating populations was complicated by the presence of a suppressor gene in Thatcher and the high temperature sensitivity of resistance expression for this gene. The effective leaf rust resistance in Alsen is due to the interaction of Lr13 and Lr23, with Lr34; and the effective leaf rust resistance in Norm is due to the interaction of Lr13, Lr16, and Lr23, with Lr34. 相似文献
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为明确春小麦品种沈免2063所含抗叶锈病基因的对数、身份、显隐性和互作关系,以沈免2063为父本,分别与感病品种Thatcher及小麦抗叶锈病近等基因系Lr9、Lr19、Lr24、Lr25、Lr28、Lr42和Lr43的载体品系杂交,获得F1、F2和F3代群体后,分别在苗期和成株期进行抗病性测定。结果表明:沈免2063含有3对显性遗传且相互独立作用的抗叶锈病基因Lr9、Lr19和Lr25,在苗期,沈免2063对致病类型CBG/QQ的抗病性由Lr9和Lr25控制,对PHT/RP的抗病性由上述3对抗叶锈病基因控制;在成株期,沈免2063对优势致病类型PHT/RP和THT/TP等比混合菌种的抗病性由上述3对抗叶锈病基因控制。Lr9、Lr19和Lr25在育成品种中出现频率很低,目前尚很有效,但这3个基因均为典型的垂直抗病性基因,应进行基因布局、基因轮换等科学组配,才能延长其使用寿命。 相似文献