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van den Hoven R Duvigneau JC Hartl RT Gemeiner M 《Veterinary research communications》2006,30(8):921-928
On four occasions, four horses with heaves and four horses with small airway inflammatory diseases inhaled 0.9% saline based
aerosol mixtures with or without lipopolysaccharides (LPS). Prior to the first saline and LPS inhalation, horses were untreated,
while three and a half days prior to the third and forth inhalation horses had received 0.8 μ g/kg clenbuterol intravenously
twice daily.
The messenger RNA (mRNA) expression of tumour necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-4, IL-6, IL-8, IL-10 and
interferon- γ (IFN- γ) was investigated by RT-PCR, all of which were expressed in the white blood cells of samples collected.
Inhalation of LPS only changed the cytokine expression profile of IL-10, IL-4 and TNF-α mRNA which were higher after challenge
with LPS. However in those horses that were treated with clenbuterol the LPS-induced IL-10 mRNA expression was shown to be
suppressed. Further changes in IL-4 and TNF-α were not significant. Thus the results of this study indicated that clenbuterol
can modulate the expression of IL-10 mRNA in peripheral white blood cells in those horses with small airway diseases that
have been exposed to LPS.
van den Hoven, R., Duvigneau, J.C., Hartl, R.T. and Gemeiner, M., 2006. Clenbuterol affects the expression of messenger RNA
for interleukin 10 in peripheral leukocytes from horses challenged intrabronchially with lipopolysaccharides. Veterinary Research
Communications, 30(8), 921–928 相似文献