褶皱臂尾轮虫工厂化高密度培养技术

利用室内水泥池总计580 m3进行了轮虫大水体、高密度培养,三年共采集轮虫6600亿个,培养密度达到200个/ml以上,较好地满足了虾、蟹、鱼类等苗种生产所需,提高了苗种的成活率,且效益显著。     

褶皱臂尾轮虫的敞池增殖

池塘的底泥中蕴藏着丰富的轮虫休眠卵,搅动底泥使其上浮可促进萌发、繁衍形成轮虫种群数量高峰期。高峰期的生物量和持续的时间与水温、食物、pH等诸多因子有关,其中饵料浮游植物的种、量是重要影响因子。在大型浮游植物长期占优势的水体,轮虫种群数量增长极慢。作者还根据轮虫的现存量估算了轮虫池的供饵能力。     

褶皱臂尾轮虫室内高密度培养技术

褶皱臂尾轮虫属轮虫纲、单巢目、游泳亚目、臂尾铃虫科。它大小适口（100～300μm）,营养丰富,并能保持良好的育苗木质,已成为鱼类、虾蟹走人工育苗中优良的动物性饵料,在生产中需求量很大,由于浙南地区的铃虫天然资源不足,不能满足生产需要,特别是在河蟹人工育苗时,外界气温低,空外大面积培养铃虫繁殖速度慢、培养密度低等因素,有必要进行室内水泥池高密度培养。作者现勾结合1995～1997年三年的河匠人It苗中的铃虫培养方法作如下介绍．1．材料与方法（1）轮虫种由相歧臂尾轮虫冬卵孵化而成。（2）培养耷器与培养池1000～3000Ynl…     

褶皱臂尾轮虫规模化培养新模式

褶皱臂尾轮虫(Brachionus plicatilis)(下文简称轮虫)隶属于轮虫纲、单巢目、臂尾轮虫科;是广盐性种类,广泛分布于温带到热带半咸水和海水水域。它是目前唯一能集约化培养的一种海水轮虫。作为海产苗种良好的开口饵料,随着近年来海产鱼类养殖和人工育苗工作的深入开展,其规模化培养技术愈发显示出重要作用。     

褶皱臂尾轮虫的高密度培养

本文针对冬季育苗生物饵料缺乏,褶皱臂尾轮虫幼体成活低的问题,采用室内高密度培育轮虫的方法,提高产量.赣榆县广星育苗场,25#～35#池用于轮虫培养,38 m2/池,1#、2#、3#池饵料培育.     

用酵母高密度培养褶皱臂尾轮虫

用面包酵母室内高密度培养褶皱臂尾轮虫,结果发现,1.5 g/(106·d)的较高投饵量.适合于接种密度约为50个/ml的褶皱臂尾轮虫培养,而投饵量为1.0～1.2 g/(106·d)时.适合于200个/ml以上较高密度的褶皱臂尾轮虫培养.投饵量为1.5 g/(106·d)时.不适于500个/ml以上接种密度的褶皱臂尾轮虫培养.若褶皱臂尾轮虫密度继续高至1000个/ml以上,投喂量则需进一步降低至1 0 g/(106·d)以下.褶皱臂尾轮虫接种密度低至30个/ml以下时,除酵母外,适当添加一定量(1/3以上体积)的单胞藻.可提高褶皱臂尾轮虫的增殖速度,降低褶皱臂尾轮虫培育的风险.     

褶皱臂尾轮虫(Brachionus Plicatilis)室内高密度培养技术

<正> 褶皱臂尾轮虫(以下简称轮虫)属轮虫纲(Rotatoria)、单巢目(Monogononta)、游泳亚目(Ploima)、臂尾轮虫科(Brachionidae)。它营养丰富,大小适口(100～300μm),是鱼类、虾蟹人工育苗中优良的动物性饵料,在育苗生产中需求量很大。由于浙南地区的轮虫天然资源不足,不能满足生产需要,特别是在河蟹人工育苗期间,外界气温低,在室外大面积培养轮虫繁殖速度慢、密度低,需     

投喂酵母和扁藻类培养褶皱臂尾轮虫的效果试验

实验室条件下，分别以面包酵母和扁藻为饵料培养褶皱臂尾轮虫（以下简称轮虫）。结果表明，轮虫摄食扁藻的生长繁殖效果要好于摄食酵母。以扁藻为食物，其带卵率和带卵数分别为45．5％和3．1个，而以酵母为食物，其带卵率和带卵数仅分别为32．5％和2．2个。在轮虫接种密度情况下（经一个培养周期7天），轮虫密度分别为54个／ml、32．5／ml。     

褶皱臂尾轮虫（Brachionus plicatilis）饲料研究的概况

褶皱臂尾轮虫（Ｂｒａｃｈｉｏｎｕｓｐｌｉｃａｔｉｌｉｓ）饲料研究的概况袁成玉吴春平（辽宁省海洋水产研究所,大连１１６０２３）褶皱臂尾轮虫（Ｂｒａｃｈｉｏｎｕｓｐｌｉｃａｔｉｌｉｓ）（以下简称轮虫）饲料的研究已有３０多年的历史。至今,国内外仍有许多学者...     

铜锌离子对褶皱臂尾轮虫的急性毒性试验

研究了Ｃｕ＋＋、Ｚｎ＋＋对褶皱臂尾轮虫（Ｂｒａｃｈｉｏｎｕｓｐｌｉｃａｔｉｌｉｓ）的急性毒性影响。实验温度２５±１℃,轮虫对Ｃｕ＋＋的污染相当敏感,Ｃｕ＋＋２４ｈ半致死浓度为２４ｈＬＣ５０＝１４．４７μｇ／Ｌ～２１．９３μｇ／Ｌ;Ｚｎ＋＋２４ｈ半致死浓度为２４ｈＬＣ５０＝３．４０４ｍｇ／Ｌ～４．５４９ｍｇ／Ｌ。     

Nitrogen Budget for the Rotifer Brachionus plicatilis

Nitrogen ingested by the rotifer, Brachionus plicatilis (S type) is balanced by gain in body nitrogen (including reproduction), soluble excreted nitrogen, and nitrogen as egested feces. Every term in the budget was estimated independently, and measured as directly as possible. A comparison of the observed and calculated nitrogen as egested feces yielded a satisfactory result.
The nitrogen ingested by the rotifer was distributed as follows: 15.6% for growth and reproduction, 42.0% for metabolic excretion, and 42.4% as feces. From these results, it was concluded that a large proportion (84%) of the nitrogen ingested by the rotifer is transferred to water in various forms and may cause deterioration of the water quality.     

Nutritional Properties of the Marine Rotifer Brachionus plicatilis Fed the Freshwater Microalgae Selenastrum capricornutum

This study examined the effects of storage time on the fatty acid composition of freshwater Selenastrum capricornutum algal paste under 4 C refrigeration, the fatty acid composition of rotifers fed the fresh and stored algal paste, and the toxicological properties of the algal paste. Microalgae were produced in a hydraulically integrated serial turbidostat algal reactor (HIS-TAR), harvested as a paste, refrigerated and analyzed every 2 wk. Fresh Selenastrum capricornutum paste had almost three times greater concentration of unsaturated fatty acids than saturated fatty acids. Over 50% of the unsaturated fatty acids were made up of n-3 and n-6 fatty acids. Total unsaturated, n-3 group and n-6 group fatty acids decreased ( P 0.05) during storage, including the nutritionally important fatty acids (C20:4n6, C20:5n3, C22: 6n3). Rotifers fed 2– and 4-wk-old algal paste had a significantly lower ( P 0.05) total unsaturated fatty acid percentage and significantly greater ( P 0.05) total saturated fatty acids than those fed fresh algae. There were no significant changes in the n-6 group fatty acid level in the 4-wk-old paste feeding study or for the n-3 or n-6 groups in the 2-wk-old paste feeding study as compared to fresh algae. The levels of the three nutritionally important fatty acids (C20:4n6, C20:5n3, C22:6n3) did not differ between rotifers fed fresh and stored algae. Stored algae did not present toxicity to rotifers and Daphnia at the normal feeding concentration. These results indicated that the use of refrigerated freshwater algal paste for production of rotifers results in live feed with adequate nutritional properties for marine larviculture. This could eliminate the costs associated with production of marine algae, which could be replaced with freshwater algae, and may provide an alternative to cryopreservation.     

Optimum Feeding Rate of the Rotifer Brachionus plicatilis on the Marine Alga Nannochloropsis sp.

The optimum feeding rate of the rotifer Bruchionus plicutilis was investigated to determine the best conditions for growth of the rotifer, and also in order to maintain good water quality of the culture. Fifty rotifers of a large size strain were cultured individually at five food density levels of Nannoehloropsis sp. (0.5 ± 10⁶, 1.5 ± 10⁶, 3 ± 10⁶, 5 ± 10⁶, and 8 ± 10⁶ cells/ml). At each level, daily survival and offspring production were recorded until the death of the final individual. The data obtained were analyzed by the life table method. The maximum value of three growth indices (the net reproduction rate, mean life expectancy at age 0, and intrinsic rate of increase) was obtdned at the food density of 1.5 ± 10⁶ cells/ml. The ration size was calculated to be 325% (dry weight)/ day, which is equivalent to about 70% of the saturated feeding rate. It was suggested that the feeding rate should be controlled to lower than the saturated value for an efficient mass culture.     

几种单胞藻对褶皱臂尾轮虫的营养强化研究

利用气相色谱法测定了小球藻、牟氏角毛藻、球等鞭金藻和投喂不同藻类后12h的褶皱臂尾轮虫的脂肪酸含量。试验结果表明,不论是投喂单种藻类还是2种混合藻类,强化后的轮虫其体内的脂肪酸含量均发生了明显变化,其中单链脂肪酸及低度不饱和脂肪酸的含量在投喂前后没有变化,各种脂肪酸的含量界于未强化的轮虫和所投喂的单胞藻饵料含量之间。3种高度不饱和脂肪酸的含量,除了二十二碳六烯酸因在牟氏角毛藻中含量较低,在单独投喂牟氏角毛藻后的轮虫中未检测出外,其余均与其所投喂的单胞藻的脂肪酸一致;轮虫中被检出的3种高度不饱和脂肪酸的含量,在单种藻类试验组中均低于所投喂藻类的含量;在2种藻类混合试验组中界于2种单胞藻含量之间。混合藻类比单种藻类强化轮虫具有更加全面的营养效果。     

褶皱臂尾轮虫培养若干问题探讨

本文就轮虫生产性培养过程中接种密度、投饵量、水质、轮虫生长和营养强化等方面的问题进行探讨,认为:接种密度在用单胞藻培养时应为10～30个/ml,用酵母培养时20～50个/ml;酵母投喂量0 3～0 35g/百万,投喂量不宜过大;酵母投喂的轮虫使用前2d最好用单细胞藻类强化。     

营口地区轮虫资源调查

通过实地调查,指出了营口地区轮虫自然资源发生的区位优势,分析了营口地区独特的生态条件,同时对轮虫的种类、产生轮虫水域的水质情况、饵料组成及其轮虫的商品特点等进行了探讨。     

养虾池排污水褶皱臂尾轮虫培养技术研究

为实现养虾污水的综合利用,结合单胞藻、褶皱臂尾轮虫(Brachionus plicatilisO.F.Muller)(以下简称轮虫)等饵料生物增养殖特性,在污水处理的同时,又能在南方批量生产水产苗种优质开口饵料,实现增收节支,课题组在2004年养虾过程中,进行了本项研究。1条件与方法1.1池塘条件与配套     

光照、pH及盐度对褶皱臂尾轮虫培养效果的影响

研究了在不同光照周期,pH和盐度突变条件下褶皱臂尾轮虫的存活和增殖率.试验结果表明,光照对褶皱臂尾轮虫种群增殖的促进作用明显,持续光照条件下轮虫种群增殖率为持续黑暗状态的3.5倍;pH存活上限9.5,下限5.5;种群增殖最适pH为7.5～8.5;种群繁殖的最适盐度为30～35,存活的下限为15,上限为40.研究结果显示,光照对于褶皱臂尾轮虫的增殖是必要的;pH的不同对褶皱臂尾轮虫带卵量、孵化率和孵化时间均有影响.盐度突变盐差越大,褶皱臂尾轮虫的适应能力(适应所需时间和存活率)越弱.