The effectiveness of various acaricides against resistant and susceptible carmine spider mites

Two colonies of the carmine spider mite,Tetranychus cinnabarinus (Boisd.), were raised on beans in the laboratory. One originated from roses in a greenhouse which had received acaricidal treatments, and the other fromViola odorata L. in a garden where no pesticides had been used. Laboratory evaluation indicated that the greenhouse strain was highly resistant to dicofol (Kelthane) and bromopro-pylate (Neoron), moderately resistant to propargite (Omite), and slightly resistant to cyhexatin (Plictran); in the garden strain, there was no resistance to any of these acaricides. On a strain of carmine spider mites which was identified and defined as resistant, several acaricides were evaluated in the laboratory for their contact toxicity to the mite by the slide dip method, and for their contact plus stomach toxicity by exposing the mites to sprayed leaves. Some of these acaricides were later tested in the field on two undetermined populations. Cyhexatin, triazophos (Hostathion), fentrifanil (PP-199) and R-28627 killed more than 90% of the mites by both methods and were effective in the field. NC-13292 was an ovicide only. Proclonol (R-8284) had a good effect in the slide dip method but was not so effective in the leaf exposure test. The compounds tested were phytotoxic to rose foliage only, but triazophos burned all the crops tested.     

捕食性昆虫的标记信息素研究进展

捕食性昆虫的标记信息素广泛存在于鞘翅目、脉翅目、双翅目、半翅目和蜱螨目捕食者中,介导捕食性昆虫的猎物搜索、产卵、同类相残和集团内捕食,改变猎物蚜虫的生活史,因而影响捕食者控制目标害虫的能力。标记信息素由幼虫、成虫或卵产生,来源于外分泌腺或消化道。目前已鉴定出几种瓢虫的标记信息素,均为烷烃混合物。捕食性昆虫对标记信息素的反应受标记信息素持效期、昆虫本身状况如产卵习惯、年龄、经历和性别及外部条件如猎物种群密度的影响。     

Check-list of fungi pathogenic to insects and mites in Israel,updated through 1992

Forty-five entomopathogenic fungal species and 215 host-pathogen combinations have been observed in Israel during the last two decades.Pathogen-host combinations are given in a list, followed by seasonal occurrence, geographic distribution and host-plant data.Host-pathogen combinations are given in a separate list, followed by the most relevant literature citations. Both lists are indexed. Recent changes in systematics and nomenclature, and seasonal and host preferences are discussed for selected species or groups.     

Distribution and migration of insects and mites in flat storage containing wheat

Studies were conducted in a flat storeroom in central Greece, filled with approx. 90 tons of wheat, in order to evaluate insect and mite species abundance, and changes in population density and distribution among sampling units and locations. The surface of the grain bulk was divided into two sampling zones: the central and the peripheral (edge). At 10-day intervals, five wheat samples were taken from the central zone and eight from the peripheral zone, with a non-partitioned grain trier (1.6-m length, 250-g capacity). Fifteen insect species and 12 mite taxa were found during the sampling period, (June 1999–Feb. 2000). The most abundant insect species wereSitophilus oryzae (L.),Cryptolestes ferrugineux (Stephens),Tribolium castaneum (Herbst) andLatheticus oryzae Waterhouse; the most abundant mite species wereAcarus siro L.,Lepidoglyphus destructor (Shrank), and the predatorsBlattisocius tarsalis (Berlese) andB. keegani Fox. Grain temperature decreased during the storage period, more slowly in the central than peripheral zone; grain moisture content increased. The highest population densities for both insects and mites were recorded during September and October. The most numerous species of insects and mites showed an aggregated spatial pattern, as indicated by Iwao’s Patchiness Regression.     

Host plant preference of aphids, thrips and spider mites on GNA-expressing and control potatoes

The effect of snowdrop lectin (Galanthus nivalis agglutinin: GNA) expression on host-plant preference was examined with a two-choice disc test. Discrimination between a transgenic potato [Solanum tuberosum (Solanaceae)] capable of synthesizing GNA, and an isogenic cultivar, was studied under laboratory conditions using the glasshouse potato aphidAulacorthum solani (Hemiptera: Aphididae), the onion thripThrips tabaci (Thysanoptera: Thripidae), and the two-spotted spider miteTetranychus urticae (Acari: Tetranychidae). Adults of these species were placed individually on leaf discs (one half transgenic and one half control) and observed 1, 24, 48, 72 and 96 h after release, at which times also the distributions ofA. solani nymphs andT. urticae eggs on the discs were recorded. Aphids were found more frequently on control leaves at all observation periods, with the highest bias observed at 72 h (only 35.9% of all aphids were found on the GNA halves). Similarly, more aphid nymphs (57.6% of the total) were found on isogenic half-discs. The percentage of thrips found on GNA halves ranged from 36.8% to 48.4%, with significant control-potato bias at the first three observations. Contrary to this, the proportion ofT. urticae females found on GNA halves was significantly lower than the expected proportion (0.5) at 72 and 96 h, with a minimum of 34.0% on GNA. The distribution of spider mite eggs reflected the observed biased distribution of females (only 34.1% of all eggs were laid on GNA half-discs). These results indicate that potatoes expressing GNA for resistance against aphids are less preferred than an isogenic cultivar by all three tested species under a choice test condition using excised leaves.     

The toxic and anti-feedant activity of 2H-pyridazin-3-one-substituted 1,3,4-oxadiazoles against the armyworm Pseudaletia separata (Walker) and other insects and mites

The toxicities and anti-feedant activities of thirteen asymmetrical 1,3,4-oxadiazoles containing a 2H-pyridazin-3-one group were investigated. The compounds were shown to possess considerable activity in retarding the development of larvae of a number of Lepidoptera, but they were all inactive against Homoptera, Diptera and Acarina. The compounds had powerful anti-feedant activity comparable with that of azadirachtin. The toxic symptoms of the poisoned larvae indicated that the compounds were novel insect growth regulators with a mode of action that might be similar to the chitin-synthesis inhibition of oxadiazole compounds and/or the juvenile hormone effect of pyridazinone compounds.     

棉叶螨的抗药性现状与治理策略

棉叶螨也称为棉红蜘蛛,属蛛形纲叶螨科,其种类繁多,分布范围广,世代周期短,是为害棉花的一类重要害螨。目前,用于防治棉叶螨的化学药剂主要是神经毒剂及呼吸抑制剂2大类,且棉叶螨对多数药剂产生了不同程度的抗性,以二斑叶螨Tetranychus urticae为首的植食性害螨已成为世界上抗药性最严重的节肢动物之一。美国路易斯安那州棉田二斑叶螨种群对阿维菌素产生了1 415倍抗性,而国内棉花上棉叶螨主要对有机磷类药剂产生了较强抗性,最高为467倍。棉叶螨产生抗药性的机制主要涉及靶标突变及解毒代谢增强,其中靶标突变主要涉及乙酰胆碱酯酶、电压门控钠离子通道和谷氨酸门控氯离子通道等;细胞色素P450单加氧酶、羧酸酯酶和谷胱甘肽S-转移酶等一种或多种解毒酶共同参与害螨对化学药剂的解毒代谢。该文主要从棉叶螨的种类及分布、用于防治棉叶螨的化学药剂、棉叶螨的抗药性现状、抗药性机制解析和抗药性治理策略5个方面进行阐述,提出因地制宜的抗药性治理策略,旨在为棉叶螨的田间防治提供指导。     

Species composition and seasonal-occurrence of spider mites and their predators in sprayed and unsprayed apple orchards in Tokat, Turkey

The species composition and seasonal cycle ofTetranychus urticae Koch (two-spotted spider mite) andEotetranychus uncatus Garman (garman spider mite; the two species were evaluated together),Amphitetranychus viennensis (Zacher) (hawthom red spider nite),Panonychus ulni (Koch) (European red mite),Bryobia rubrioculus (Scheuten) (brown mite) (Acari Tetranychidae),Cenopalpus pulcher Can and Fanz. (flat scarlet mite) (Tenuipalpidae) and their natural enemies (Phytoseiidae and Stigmaeidae family members) were compared in two apple orchards during the years 2000 to 2002 in Tokat, Turkey. One orchard was pesticide-free, and the other was sprayed. Surveys were carried out once a week from April to November and the differences in the dominant species of spider mites and phytoseiid mites between the two orchards were recorded. In the sprayed orchard, the dominant phytophagous mites wereA. vienensis andP. ulmi whereas in the unsprayed orchardE uncatus andC pulcher were abundant. It was found that the predatory mites were able to control spider mites in the unsprayed orchard, but the population level was not adequate to control them in the sprayed orchard, presumably due to the excessive use of pesticides.     

Studies on insects and mites causing galls on the leaves of elm Ulmus laevis Pall. in the Mogilski Forest in southern Poland

Species composition of galls on the leaves of Ulmus laevis Pall. was analysed in 2001–02 in the Mogilski Forest – near an urban agglomeration of Kraków. A total of 5830 galls were found. The galls were caused by 5 insect or mite species. Aceria ulmicola brevipunctata (Nalepa) was the most abundant species in both years.The index of occurrence frequency and Agrell's index of species co-occurrence have been calculated. The index of occurrence frequency (F) reached the highest value in the case of A. ulmicola brevipunctata, namely 1.740, in 2001, and the lowest value, i.e. 0.00005 for Schizoneura ulmi (Linné), in 2001. Agrell's index of species co-occurrence reached the highest values, i.e. 0.500, in the case of A. ulmicola brevipunctata and Physemocecis ulmi (Kieffer), in 2002, and the lowest one, namely 0.050 for A. ulmicola brevipunctata and Schizoneura ulmi, in 2001.     

Potential of a novel antibiotic, 2-methylheptyl isonicotinate, as a biocontrol agent against fusarial wilt of crucifers

Screening for newer bioactive compounds from microbial metabolites resulted in the isolation of a novel antibiotic from the culture filtrate, Streptomyces sp 201, of a soil. The bioactive compound, with antifungal and antibacterial activity, was identified as 2-methylheptyl isonicotinate. The antifungal activity of live culture, culture broth and the isolated bioactive compound showed marked inhibition against dominant soil-borne phytopathogens such as Fusarium oxysporum Schlect, F moniliforme Sheldon, F semitectum Berkeley & Ravenel, F solani (Martius) Sacc and Rhizoctonia solani Kuehn. The compound had no effect on seed germination and seedling development as displayed by root and stem growth of the test plant species. In pot experiments with seedlings of cruciferous plants such as Raphanus sativus L (radish), Brassica campestris L (yellow mustard), Brassica oleracea var botrytis L (cauliflower), the antibiotic compound showed promising protective activity of 92% when seeds of the test plants were treated at a dose of 50 micrograms ml-1 prior to sowing. Seed treatment with a spore suspension (3 x 10(8) spores ml-1) of the Streptomyces sp 201 displayed protective activity in the range of 56-60%. Seeds coated with 2.5% methyl cellulose-amended spores of the antagonist showed protective activity in the range of 64-72%. Further, seed treatment with the culture filtrate of the antagonist also showed promising protective activity in the range of 64-84%.     

多炔类化合物对稗草光活化生长抑制活性及作用靶标

采用小杯法测定了8个人工合成的多炔类化合物对稗草的光活化生长抑制活性,经近紫外光(320～400nm)照射后,所测的化合物中的化合物5(1-苯基-4-(3,4-亚甲基二氧)苯基-丁二炔)能显著抑制稗草根、茎的伸长,当处理浓度为100mg/L时,经光照处理测得对稗草根、茎生长抑制率分别为93.56%和68.03%,而非光照处理则为27.73%和17.76%,显示出明显的光活化生长抑制效应.在检测不同光照时间和不同浓度处理的试验结果中发现,随着光照时间从0.5h到3h,浓度从0.1mg/L到10mg/L时,测得稗草根长和茎长的抑制率呈现明显的正相关.电镜下观察表明,经化合物5处理的稗草愈伤组织,浓度为10mg/L、光照为3h时,可造成生物膜结构的破坏,而非光照的细胞内膜结构受到轻度的损伤.     

Studies on insects and mites causing galls on the leaves of hornbeamCarpinus betulus L. in the Ojców National Park in Poland

Analytical studies were conducted of the species composition of galls occurring on the leaves ofCarpinus betulus L. in the Ojców National Park in the years 1995–1996. Galls were caused by 3 species:Aceria macrotricba (Nalepa),Zygiobia carpini (F. L?w) andZ. ruebsaameni Stelter (a total of 631 galls). Index of occurrence frequency and Agrell's index of species co existence have been calculated.Z. ruebsaameni appeared to be a new species for the fauna of Poland. Part of investigations under the problem BW 2059/KEL/95-96.     

Studies on insects and mites causing galls on the leaves of lime treeTilia cordata Mill. in the Ojców National Park in Poland

Analytical studies were conducted on the species composition of galls occurring on the leaves ofTilia cordata Mill. in the Ojców National Park in 1997–1998. The species composition of galls was identical in two years. Galls were caused by 6 species. The most numerous species wereEriophyes leiosoma (Nalepa) in 1997 andPhytoptus tetratichus tetratrichus Nalepa in 1998. Index of occurrence frequency and Agrell’s index of species co-existence have been calculated. This index reached the highest values in both years in case ofE. leiosoma andP. tetratrichus tetratrichus.     

北京地区园林植物病虫害可持续控制方法的探讨

以生态平衡理论为基础,结合目前北京地区园林植物病虫害防治现状,从建立机构、立法、植物检疫、生物防治、化学农药的合理使用等方面提出了解决园林植物病虫害综合防治及可持续控制的管理对策和技术措施。     

Surfaces and other factors modifying the effectiveness of pyrethroids against insects in public health

Features of the evolution of synthetic pyrethroid insecticides are reviewed and related to the target function in control. The factors affecting residual activity are discussed and related to the surfaces to which different formulations are applied. Many insecticide/formulation/surface combinations lose effectiveness rapidly. The consequence of this in relation to the recovery of cockroach populations are discussed using mathematical models and field observations.     

Natural product chemistry and its part in the defence against insects and fungi in agriculture†

This paper surveys our work on natural products as potential models for defensive substances against insect and fungal predators. Insecticides and repellents included are pyrethrins, rotenoids, lipid amides, phorbol esters, cordifolia germacranolides, nicandrenoids, mammeins, dihydroagarofuran esters, and cembrene diols. The fungal H-S toxins from Alternaria, and avenacins from oat roots are briefly considered. The avenacins provide an in-situ defence of oat roots against the destructive ‘Take-all’ fungus disease. © 1999 Society of Chemical Industry