Impact of Nitrogen and Plant Growth Promoting Rhizobacteria on yield and yield components of sunflower in a glasshouse environment

The effects of Nitrogen (N) and Plant Growth Promoting Rhizobacteria (PGPR) on growth and development of sunflower (Helianthus annuus L. var. Hysun-33) grown in the greenhouse under a natural environment were studied. The N-use efficiency of a sunflower crop grown under three N rates (N₁ = 0 kg ha^?1, N₂ = 120 kg ha^?1, and N₃ = 240 kg ha^?1) and three PGPR levels (R₁ = 0 kg ha^?1, R₂ = 30 kg ha^?1, and R₃ = 60 kg ha^?1) were investigated. The maximum amount of N resulted in higher total dry matter production per plant and the effect was prominent from 34 days after sowing (DAS). Seed yields differed significantly among different sunflower crops especially at limiting N supply, with significant shifts according to the N level. N uptake was an important parameter for yield at all N rates. The 240 kg N ha^?1 treatments provided the maximum yield, while the oil contents in these treatments of higher yield showed a lower oil content (%). Harvest index was also significantly correlated to yield across N rates; however, its importance depended much on environmental conditions as well. It can be inferred from the study that sunflower crop is well-supplied with respect to growth, development, yield and yield components, to enhance N efficiency and depends very much on the N supply. All the parameters gave maximum results with the increment of N while PGPR regimes had no prominent impact on the sunflower crop, the target environment, and the target yield level grown under a specified controlled glasshouse environment.     

Effects of Time and Rate of Nitrogen and Phosphorus Application on the Growth and the Seed and Oil Yields of Canola (Brassica napus L.)

A field study was conducted to investigate the influence of variable rates of application of N and P fertilizers in splits at various times on the growth and the seed and oil yields of canola (Brassica napus L.) during 1995–97. Rates of fertilizer application were 0 and 0 (F0), 60 and 0 (F1), 0 and 30 (F2), 60 and 30 (F3), 90 and 60 (F4) and 120 and 90 (F5) kg N ha^?1 and kg P₂O₅ ha^?1. All the P was applied at sowing while N was applied in splits, i.e. all at sowing, half at sowing and half with first irrigation, or half at sowing and half at flowering. The responses of growth, seed yield and components of yield were consistent in both years. Increasing the rate of fertilizer application from F4 (90/60 kg N/P₂O₅ ha^?1) to F5 (120/90 kg N/P₂O₅ ha^?1) increased the leaf area index (LAI) relative to the control and to lower rates of fertilizer application. For both crops, application of 90/60 kg N/P₂O₅ ha^?1 significantly enhanced total dry matter (TDM) and seed yield. Seed yield increased mainly due to a greater number of pods per plant and seeds per seed‐pod. The time of fertilizer application did not significantly affect seed yield or components of yield in either season. Oil yield generally followed seed yield, increasing with increasing rate of fertilizer application up to 90/60 kg N/P₂O₅ ha^?1. The maximum oil contents were obtained from the control. The results show that seed and oil yields of canola were maximized at the F4 (90/60 kg N/P₂O₅ ha^?1) rate of application under the agro‐ecological conditions of Faisalabad, Pakistan.     

Nitrogen and Phosphorus Effects on Faba Bean Yield and Some Yield Components

The faba bean is among the major grain legumes cultivated in Ethiopia and is used extensively as a break crop in the highlands. Although a blanket application of DAP (diammonium phosphate) at the rate of 100 kg · ha^?1 has been practised in faba bean production in the country, this was not based on research results. In addition, little information is available on the response of the crop to N and P fertilizers under diverse environmental conditions. Hence, field experiments were carried out at three locations in 1991, seven locations during 1992 and 1993 and at one location in both 1993 and 1995 to determine faba bean response to N and P fertilization. Five levels of N (0, 9, 18, 27 and 36 kg N · ha^?1 as urea) in factorial combinations with four levels of P (0, 23, 46 and 69 kg P₂O₅ · ha^?1 as TSP [triple super phosphate]) were studied in a randomized complete block design with four replications in the first year. In the remaining years four levels of N (0, 18, 27 and 36 kg N · ha^?1 as urea) in factorial combinations with four levels of P (0, 23, 46 and 92 kg P₂O₅ · ha^?1 as TSP) were used in a randomized complete block design with three and four replications at one and seven locations, respectively. Results indicated that a positive linear response of faba bean seed yield was noted at all locations (except Debre Zeit and Burkitu) to P fertilization, while a significant quadratic response was also found at Holetta. In addition, plant height, above ground biomass and number of pods per plant were positively influenced by P application while the effect of N on these was mostly nonsignificant. Faba bean seed yield response to N was noted at only two out of eight locations; in most cases, nonsignificant and inconsistent seed yield responses to N fertilization were obtained. There was nonsignificant N × P rate interaction. In conclusion, we do not recommend supplemental N application to faba bean at six out of eight locations but we recommend the application of P fertilizer to faba bean at almost all locations (with the exception of Debre Zeit) and for other soils deficient in available P. Further work is recommended on the determination of critical levels for soil-available P, below which P fertilization should be practised for optimum faba bean seed yield.     

Maize Grain Yield Response to Tillage and Fertilizer Nitrogen Rates on a Tara Silt Loam

Effects of tillage on the appropriate fertilizer N applications needed to achieve maximal grain yield are poorly denned. The study objective was determination of relative corn grain yield response to N application rate for four tillage practices: no-tillage (NT), ridge tillage (RT), fall chisel plowing (CP) and fall moldboard plowing (MP). Maize (Zea mays L.) grain yield and N accumulation were monitored over a 6 year period with the same tillage treatment and the same fertilizer N rate applied each year to each plot. Two hybrids, differing in relative maturity rating, were planted each year. Fertilizer N rates ranged from 10 to 190 kg ha^?1 and consisted of 10 kg ha^?1 of liquid starter N applied at planting with varying amounts of fall applied anhydrous ammonia. With only starter fertilizer, grain yields increased with tillage intensity in the order NT ≤ RT ≤ CP ≤ MP. With ≥ 55 kg total applied Nha^?1, 6 year average grain yields were unaffected by tillage. Total N removed in grain annually with only starter fertilizer ranged from 25–85 kg ha^?1 Maximal amounts of N removed, about 145 kg N ha^?1, occurred with 100–145 kg applied N ha^?1 for all tillage treatments under the more favorable climatic conditions. Several interactions affecting grain yield appear climatically sensitive with exception of tillage by fertilizer N interactions. Because of variability in climate, planting dates varied by almost 4 weeks. Relative yield loss due to planting delay were Fertilizer N (mean change ??124 –?275 kg ha^?1 day^?1) > Starter N only and MP (mean ?? 259 kg ha^?1 day^?1) > other tillages in general. Yield loss due to delayed planting ranged from 0.0–275 kg ha^?1 day^?1. Grain yield gains due to early spring soil temperatures were 16.0–21.8 kg ha^?1 index-degree^?1 with MP tillage and averaged 2.7– 16.7 kg ha^?1 index-degree^?1 more than those of other tillage-hybrid combinations.     

Impact of Phosphorus from Dairy Manure and Commercial Fertilizer on Perennial Grass Forage Production

Increased recovery and recycling of manure phosphorus (P) by crops on dairy farms is needed to minimize environmental problems. The main objective of this study was to compare P utilization by orchardgrass (Dactylis glomerata L.) and tall fescue (Festuca arundinaceae Schreb.) from dairy manure or inorganic fertilizer. The study was conducted from 1994 to 2000 at the Cornell University Baker Farm, Willsboro, NY, on a somewhat poorly drained Kingsbury clay (very–fine, illitic, mesic Aeric Epiaqualfs). The design was a split‐plot in a randomized complete block with two manure rates (16 800 and 33 600 kg ha^?1) and one nitrogen (N) fertilizer rate (84 kg N ha^?1 at spring greenup and 56 kg N ha^?1 prior to each regrowth harvest) as the main plots and grass species as subplots replicated six times. Fertilizer P [Ca(H₂PO₄)₂] was applied to the fertilizer treatment in 1995 and 1996 at 11 kg P ha^?1 year^?1. Orchardgrass P removal averaged 21 % higher than tall fescue P removal for the spring harvest, but orchardgrass averaged 24 % lower P removal than tall fescue removal for all regrowth harvests from 1995–99. Phosphorus herbage concentration in the fertilizer treatment was in the range of 1.9–2.7 g P kg^?1 compared with 2.2–5.3 g P kg^?1 in the manure treatments. Seasonal P removal ranged from as low as 9.2 kg P ha^?1 to as high as 48.5 kg P ha^?1. Morgan extractable soil P in the top 0–0.20 m remained high through 1999, with 29.1 kg P ha^?1 at the highest manure rate in tall fescue compared with 8.4 kg P ha^?1 measured in 1993 prior to the experiment. In 2000, soil P at the highest manure rate in tall fescue dropped to 10.1 kg P ha^?1, following cessation of manure application in 1998. Intensively managed harvested orchardgrass and tall fescue have the potential to remove large quantities of manure P.     

氮肥用量及钾肥施用对稻麦周年产量及效益的影响

为探明优化施氮量与高施氮量下不同钾肥施用处理对稻麦周年产量及效益的影响。本试验于2010年5月–2011年7月在江苏省如皋市农业科学研究所试验基地的田间稻麦轮作条件下,对常规粳稻品种镇稻11和春性中筋品种扬麦16设置了两个氮肥用量下不同钾肥用量及施用方法处理,测定稻麦周年的产量和组成因子,成熟期不同器官的氮、钾浓度和累积量,氮、钾利用效率及经济效益。试验结果表明,钾肥的施用显著提高了周年稻麦的产量,同时提高了稻麦的有效穗数、穗粒数和结实率,钾肥的利用效率和经济效益。稻麦周年钾肥(K₂O)的偏生产力(PFP)、农学效率(AE)、回收利用率(RE)和经济效益均以周年钾肥(K2O)土壤施用150 kg hm^-2 + 叶面喷施16.2 kg hm^-2 (K_S150 + K_F16.2)处理最高。氮肥用量的结果表明,相对于优化施氮量,高施氮量有利于提高水稻的氮素营养而增产,但对稻麦周年产量的影响不显著,且优化施氮量的氮肥利用效率及经济效益均高于高施氮量。因此,综合考虑土壤环境因素、经济效益和肥料资源管理,本地区最佳氮肥(N)用量为水稻200 kg hm^-2,小麦180 kg hm^-2;最佳钾肥(K₂O)用量及方法为水稻土壤施用90 kg hm^-2 + 叶面喷施9.7 kg hm^-2 (K_S90 + K_F9.7),小麦土壤施用60 kg hm^-2 + 叶面喷施6.5 kg hm^-2 (K_S60 + K_F6.5)。     

Productivity and Sustainability of Cotton (Gossypium hirsutum L.)–Wheat (Triticum aestivum L.) Cropping System as Influenced by Prilled Urea, Farmyard Manure and Azotobacter

Field experiments were conducted at Indian Agricultural Research Institute, New Delhi, during 2001–2002 and 2002–2003, to study the effect of inorganic, organic and Azotobacter combined sources of N on cotton (Gossypium hirsutum L.) and their residual effect on succeeding wheat (Triticum aestivum L.) crop. The results indicated considerable increase in yield attributes and mean seed cotton yield (2.33 Mg ha^?1) with the combined application of 30 kg N and farmyard manure (FYM) at 12 Mg ha^?1 along with Azotobacter (M₄). The treatment in cotton that included FYM, especially when fertilizer N was also applied could either improve or maintain the soil fertility status in terms of available N, P and K. Distinct increase in yield attributes and grain yield of wheat was observed with the residual effect of integrated application of 30 kg N ha^?1 + FYM at 12 Mg ha^?1 + Azotobacter. Direct application of 120 kg N ha^?1 resulted 67.4 and 17.7 % increase in mean grain yield of wheat over no N and 60 kg N ha^?1, respectively. Integrated application of organic and inorganic fertilizer is therefore, recommended for higher productivity and sustainability of the cotton–wheat system.     

Drip Irrigation Frequency: The Effects and Their Interaction with Nitrogen Fertilization on Sandy Soil Water Distribution, Maize Yield and Water Use Efficiency Under Egyptian Conditions

Irrigation frequency is one of the most important factors in drip irrigation scheduling that affects the soil water regime, the water and fertilization use efficiency and the crop yield, although the same quantity of water is applied. Therefore, field experiments were conducted for 2 years in the summer season of 2005 and 2006 on sandy soils to investigate the effects of irrigation frequency and their interaction with nitrogen fertilization on water distribution, grain yield, yield components and water use efficiency (WUE) of two white grain maize hybrids (Zea mays L.). The experiment was conducted by using a randomized complete block split‐split plot design, with four irrigation frequencies (once every 2, 3, 4 and 5 days), two nitrogen levels (190 and 380 kg N ha^?1), and two maize hybrids (three‐way cross 310 and single cross 10) as the main‐plot, split‐plot, and split‐split plot treatments respectively. The results indicate that drip irrigation frequency did affect soil water content and retained soil water, depending on soil depth. Grain yield with the application of 190 kg N ha^?1 was not statistically different from that at 380 kg N ha^?1 at the irrigation frequency once every 5 days. However, the application of 190 kg N ha^?1 resulted in a significant yield reduction of 25 %, 18 % and 9 % in 2005 and 20 %, 13 % and 6 % in 2006 compared with 380 kg N ha^?1 at the irrigation frequencies once every 2, 3 and 4 days respectively. The response function between yield components and irrigation frequency treatments was quadratic in both growing seasons except for 100‐grain weight, where the function was linear. WUE increased with increasing irrigation frequency and nitrogen levels, and reached the maximum values at once every 2 and 3 days and at 380 kg N ha^?1. In order to improve the WUE and grain yield for drip‐irrigated maize in sandy soils, it is recommended that irrigation frequency should be once every 2 or 3 days at the investigated nitrogen levels of 380 kg N ha^?1 regardless of maize varieties. However, further optimization with a reduced nitrogen application rate should be aimed at and will have to be investigated.     

Strategies to Improve the Use Efficiency of Mineral Fertilizer Nitrogen Applied to Winter Wheat

Recovery of fertilizer nitrogen (N) applied to winter wheat crops at tillering in spring is lower than that of N applied at later growth stages because of higher losses and immobilization of N. Two strategies to reduce early N losses and N immobilization and to increase N availability for winter wheat, which should result in an improved N use efficiency (= higher N uptake and/or increased yield per unit fertilizer N), were evaluated. First, 16 winter wheat trials (eight sites in each of 1996 and 1997) were conducted to investigate the effects of reduced and increased N application rates at tillering and stem elongation, respectively, on yield and N uptake of grain. In treatment 90‐70‐60 (90 kg N ha^?1 at tillering, 70 kg N ha^?1 at stem elongation and 60 kg N ha^?1 at ear emergence), the average values for grain yield and grain N removal were up to 3.1 and 5.0 % higher than in treatment 120‐40‐60, reflecting conventional fertilizer practice. Higher grain N removal for the treatment with reduced N rates at tillering, 90‐70‐60, was attributed to lower N immobilization (and N losses), which increased fertilizer N availability. Secondly, as microorganisms prefer NH₄⁺ to NO₃^? for N immobilization, higher net N immobilization would be expected after application of the ammonium‐N form. In a pot experiment, net N immobilization was higher and dry matter yields and crop N contents at harvest were lower with ammonium (ammonium sulphate + nitrification inhibitor Dicyandiamide) than with nitrate (calcium nitrate) nutrition. Five field trials were then conducted to compare calcium nitrate (CN) and calcium ammonium nitrate (CAN) nutrition at tillering, followed by two CAN applications for both treatments. At harvest, crop N and grain yield were higher in the CN than in the CAN treatment at each N supply level. In conclusion, fertilizer N use efficiency in winter wheat can be improved if N availability to the crops is increased as a result of reduced N immobilization (and N losses) early in the growth period. N application systems could be modified towards strategies with lower N applications at tillering compensated by higher N dressing applications later. An additional advantage is expected to result from use of nitrate‐N fertilizers at tillering.     

Effect of Deep Placement of Slow-release Fertilizer (Lime Nitrogen) Applied at Different Rates on Growth, N2 Fixation and Yield of Soya Bean (Glycine max [L.] Merr.)

A new fertilization method with deep placement of slow‐release N fertilizers, such as coated urea and lime nitrogen (LN) (calcium cyanamide) at 20 cm depth was found to promote soy bean seed yield. In the present study, the effect of deep placement of LN was investigated on different parameters such as growth, N accumulation, N₂ fixation activity and yield of soy bean by applying LN at different rates in the rotated paddy field of Niigata, Japan. In addition to the basal fertilizer, ammonium sulphate (16 kg N ha^?1), deep placement of LN was conducted by applying various amounts such as 50 kg N ha^?1 (A50), 100 kg N ha^?1 (A100) and 200 kg N ha^?1 (A200) at 20 cm depth in separate plots. A ¹⁵N‐labelled LN fertilizer was also employed for each of the above treatments to calculate N utilization from LN in separate plots. Soya bean plant growth and N₂ fixation activity were periodically analysed. Both plant growth and N accumulation were found to increase with LN treatment compared with control plants. An increase in N₂ fixation activity was found in the A100 plots. The total seed yield was the highest in the deep placement of LN with A100 (73 g per plant) compared with other treatments. The visual quality of harvested seeds also showed that A100 enhanced the quality of seeds compared with other treatments. Thus, it is suggested that N fertilization management with particular reference to optimum amount of fertilizers is important for maximum growth, N₂ fixation and enhancement of seed yield of soy bean.     

Transfer of Grain Legume Nitrogen within a Crop Rotation Containing Winter Wheat and Winter Barley

In a crop rotation trial, conducted from 1985 to 1988 at TU-Munich's research station in Roggenstein, the transfer of grain legume nitrogen was evaluated in crop rotations containing fababeans and dry peas as well as oats (reference crop) and winter wheat and winter barley as following crops. The results obtained can be summarized as follows: Dinitrogen fixation by fababeans ranged from 165 to 240 kg N ha¹, whereas N₂-fixation by peas amounted from 215 to 246 kg N ha^?1. In all seasons the calculated N-balance where only grain was removed was positive, with a net gain being on average 106 (peas) and 84 (fababeans) kg N ha^?1. After the harvest of peas 202 kg N ha^?1 remained on the field on average over seasons (158 kg N ha^?1 in the above ground biomass and 44 kg N ha^?1 as NO₃-N in 0–90 cm depth). As compared to peas, fababeans left 41 kg N ha^?1 less due to smaller amounts of nitrogen in the straw. After oats very small amounts of residual nitrogen (33 kg N ha^?1) were detected. After the harvest of grain legumes always a very high nitrogen mineralization was observed during autumn especially after peas due to a close C/N-relationship and higher amounts of nitrogen in the straw as compared to fababeans. In comparison with fababeans, N-mineralization after the cultivation of oats remained lower by more than 50%. During winter, seepage water regularly led to a considerable decrease of soil NO₃-N content. The N-leaching losses were especially high after cultivation of peas (80 kg N ha ^?1) and considerably lower after fababeans (50 kg N ha^?1) and oats (20 kg N ha^?1). As compared to oats, a higher NO₃-N content in soil was determined at the beginning of the growing period after preceding grain legumes. Therefore, winter wheat yielded highest after preceding peas (68 dt ha^?1) and fababeans (60 dt ha^?1) and lowest after preceding oats (42 dt ha^?1). The cultivation of grain legumes had no measurable effect on yield formation of the third crop winter barley in either of the growing seasons.     

施氮量对晋南旱地冬小麦光合特性、产量及氮素利用的影响

在自然降水条件下,通过2年大田试验研究了施氮量对晋南旱地冬小麦光合特性、产量、氮素利用效率以及0~200 cm土层NO₃-N残留的影响。结果表明,在0~270 kg hm^-2施氮量范围内,随施氮量的增加,旗叶的净光合速率和叶绿素含量增加,气孔导度增大,胞间二氧化碳浓度降低,旗叶蒸腾速率显著提高; 但施氮量超过180 kg hm^-2时,除蒸腾速率外其他光合指标均无显著变化。N180处理的氮素当季回收率及氮素农学效率均最高,且显著高于N90处理。生物产量以N270处理最高,且与其他处理差异显著; 但施氮量超过180 kg hm^-2时,氮素营养对籽粒产量不再有显著贡献。从产量构成因素来看,提高穗数和穗粒数是增加当地旱作小麦籽粒产量的关键。施氮量90~270 kg hm^-2会造成土壤NO₃-N的残留,残留量占施氮量的35%左右,其中20~40 cm和40~60 cm土层出现NO₃-N积累峰值,NO₃-N残留会导致氮素淋失风险增加及产量对氮肥反应不明显。综合考虑光合特性、产量、氮素利用率和NO₃-N残留量,当地旱作冬小麦施氮量以180 kg hm^-2左右为宜。     

Time Course of Nitrogen in Soil Solution and Nitrogen Uptake in Maize Plants as affected by Form and Application Time of Fertilizer Nitrogen

Field experiments with silage maize were conducted in 1987 and 1988 on a loess-derived Luvisol in southwest Germany. Four nitrogen fertilizer treatments were compared: application of preplanting NH₄ N (plus a nitrification inhibitor, dicyandiamide as Didin) and preplanting NO₃-N, split application of NO₃-N (preplanting and side dressed 45 days after planting) and a control without nitrogen fertilizer in 1987 and with 64 kg N ha^?1 as calcium ammonium nitrate in 1988. The total amounts of soil mineral nitrogen (N_min+ fertilizer N) were 200 kg N ha^?1 in 1987 and 240 kg N ha^?1 in 1988. Suction cups and tensiometer were installed at five depths and samples were taken in regular intervals. Nitrate concentrations in the suction solution steeply increased at 15 cm and 45 cm soil depth 3-4 weeks after fertilizer application (1987 up to 160mgNl^?1; 1988 up to 170mgN l^?1) and steeply decreased up to 75 cm depth with the onset of intensive N uptake at shooting. Ammonium concentrations in the suction solution were very low (0-0.16 mg N l^?1). Compared to preplanting NCyN application, preplanting NH₄-N and split NO₃-N application decreased nitrate concentrations in the suction solution in spring 1987. In 1988, however, nitrate concentrations in the suction solution of preplanting NH₄-N and split NO₃-N application plots did not fall below 50mgNl^?1 at 15 cm depth during the growing season. Nitrate concentrations of split NO₃-N application increased again in autumn 1988 and hence doubled the calculated N losses by leaching during the winter months compared to preplanting N applications. At shooting, plants of the preplanting NH₄-N treatment had lower nitrate concentrations in leaf sheaths compared to plants of preplanting NO₃-N application. Total N uptake of maize between shooting and early grain filling of preplanting NH₄-N and split NO₃ -N application tended to be higher compared to preplanting NO₃-N application, reflecting the higher N availability in the soil later in the season. However, final dry matter yields and N uptake were not significantly affected by N form or time of N application. Since N losses by nitrate leaching between N application and onset of N uptake by plants were negligible on the experimental site, preplanting NH₄-N application and split NO₃-N application showed no agronomic advantages. High amounts of side dressed NO₃-N may increase nitrate leaching during the winter months, especially in years with delayed rainfall after application.     

乙烯利和氮肥对夏玉米氮素吸收与利用及产量的调控效应

以玉米品种"郑单958"为材料,在大田条件下,研究了乙烯利(0和180 g hm–2)和氮肥水平(0、75、150和225kg N hm–2)对夏玉米产量、氮素吸收和利用以及SPAD值的影响。结果表明,乙烯利处理显著降低了氮吸收量和吸收效率,但显著提高氮利用效率,其中乙烯利处理氮农学效率比对照提高了32.7%~34.6%,而且乙烯利处理对玉米产量及其产量构成因素没有显著影响;随着施氮量增加,夏玉米产量、产量构成因素和氮吸收量显著增加,而氮吸收效率、氮利用效率、氮偏生产力和氮农学效率随之降低,其中225 kg N hm–2处理氮吸收量比0 kg N hm–2处理提高了68.4%~91.8%,但225 kg N hm–2和150 kg N hm–2处理之间的氮吸收量差异不显著。乙烯利和氮肥对氮吸收量、氮吸收效率和氮农学效率具有互作效应。喷施乙烯利和增施氮肥均能提高灌浆期穗位叶SPAD值,但两者之间没有互作效应。通过相关性分析表明,夏玉米产量与吐丝期氮吸收量、收获期氮吸收量、灌浆期穗位叶SPAD值显著正相关。     

Effect of Different Crop Densities of Winter Wheat on Recovery of Nitrogen in Crop and Soil within the Growth Period

Previous experiments have shown that, at harvest of winter wheat, recovery of fertilizer N applied in early spring [tillering, Zadok’s growth stage (GS) 25] is lower than that of N applied later in the growth period. This can be explained by losses and immobilization of N, which might be higher between GS 25 and stem elongation (GS 31). It was hypothesized that a higher crop density (i.e. more plants per unit area) results in an increased uptake of fertilizer N applied at GS 25, so that less fertilizer N is subject to losses and immobilization. Different crop densities of winter wheat at GS 25 were established by sowing densities of 100 seeds m^–2 (S_low), 375 seeds m^–2 (S_cfp= common farming practice) and 650 seeds m^–2 (S_high) in autumn. The effect of sowing density on crop N uptake and apparent fertilizer N recovery (aFNrec = N in fertilized treatments ? N in unfertilized treatments) in crops and soil mineral N (N_min), as well as on lost and immobilized N (i.e. non‐recovered N = N rate ? aFNrec), was investigated for two periods after N application at GS 25 [i.e. from GS 25 to 15 days later (GS 25 + 15d), and from GS 25 + 15d to GS 31] and in a third period between GS 31 and harvest (i.e. after second and third N applications). Fertilizer N rates varied at GS 25 (0, 43 and 103 kg N ha^–1), GS 31 (0 and 30 kg N ha^–1) and ear emergence (0, 30 and 60 kg ha^–1). At GS 25 + 15d, non‐recovered N was highest (up to 33 kg N ha^–1 and up to 74 kg N ha^–1 at N rates of 43 and 103 kg N ha^–1, respectively) due to low crop N uptake after the first N dressing. Non‐recovered N was not affected by sowing density. Re‐mineralization during later growth stages indicated that non‐recovered N had been immobilized. N uptake rates from the second and third N applications were lowest for S_low, so non‐recovered N at harvest was highest for S_low. Although non‐recovered N was similar for S_cfp and S_high, the highest grain yields were found at S_cfp and N dressings of 43 + 30 + 60 kg N ha^–1. This combination of sowing density and N rates was the closest to common farming practice. Grain yields were lower for S_high than for S_cfp, presumably due to high competition between plants for nutrients and water. In conclusion, reducing or increasing sowing density compared to S_cfp did not reduce immobilization (and losses) of fertilizer N and did not result in increased fertilizer N use efficiency or grain yields.     

Response of Rice Varieties Grown in Semi-deepwater Ecosystem to Foliage Pruning and Nitrogen Fertilization

Field experiments were conducted at Cuttack, India, in 1992 and 1993 using two semi-tall (Panidhan and CR 580-5 of 180 days duration) and two tall (Amulya and CR 626-26-2-3 of 170 days duration), elongating and photosensitive rice varieties to study their response to foliage pruning at varying levels of nasally applied N fertilizer (0, 30, 60 and 90 kg N ha^?1). Pruning at the collar level of the topmost leaf was done either once at 90 or 120 days of growth, or twice at both the stages. The crop was sown during the end of May in dry soil and subsequently grown under a semi-deepwater regime (0–80 cm water depth). The dry weight of pruned foliage was more in the tall than in the semi-tall varieties and it increased with an increase in the level of N fertilization and delay in pruning. Two prunings produced greater foliage yield than one pruning. Foliage pruning once at 90 days of growth had no effect on the grain yield of all varieties. However, pruning at 120 days of growth decreased the yield of Amulya and CR 626-26-2-3, particularly in 1992, possibly because of less time (25–30 days) available for the crop recovery before flowering. Further, pruning twice at 90 and 120 days of growth reduced the grain yield of all the varieties significantly due to a reduction in their panicle weight. Application of N fertilizer beyond 30 kg N ha^?1 did not increase the grain yield under no pruning treatment, but the crop responded significantly up to 60 kg N ha^?1 under one pruning and up to 90 kg N ha^?1 under two prunings, despite its adverse effect on crop performance. The crop lodging in response to increased N application was delayed by about a fortnight due to foliage pruning but its beneficial effect was not reflected in the grain yield. The results indicated that the green leafy foliage of the tall elongating rice varieties could be harvested 40–50 days before flowering for feeding the cattle without impairing their productivity under semi-deepwater ecosystem.     

秸秆还田配施氮肥对东北春玉米光合性能和产量的影响

秸秆还田配施氮肥是解决旱作农田"耕层变浅"、"土壤紧实"、"有效耕层土壤减少"问题的重要措施之一,在旱作农业生产中具有重要意义。为探明秸秆深翻还田配施氮肥对东北春玉米光合性能和产量的影响,本研究于2014—2015年在辽宁铁岭设置S0F0(秸秆0 kg hm~(–2)+纯NPK 0 kg hm~(–2))、SN0(秸秆9000 kg hm~(–2)+纯N 0 kg hm~(–2)+纯P 112.5 kg hm~(–2)+纯K 90 kg hm~(–2))、SN1(秸秆9000 kg hm~(–2)+纯N 112.5 kg hm~(–2)+纯P 112.5 kg hm~(–2)+纯K 90 kg hm~(–2))、S0N2(秸秆0 kg hm~(–2)+纯N 225 kg hm~(–2)+纯P 112.5 kg hm~(–2)+纯K 90 kg hm~(–2),当地传统种植方式,CK)、SN2(秸秆9000kg hm~(–2)+纯N 225 kg hm~(–2)+纯P 112.5 kg hm~(–2)+纯K 90 kg hm~(–2))、SN3(秸秆9000 kg hm~(–2)+纯N 337.5 kg hm~(–2)+纯P 112.5kg hm~(–2)+纯K 90 kg hm~(–2))6个处理开展了研究。结果表明,秸秆还田配施氮肥对春玉米籽粒产量和生物产量影响显著,秸秆还田9000 kg hm~(–2)和配施氮肥225 kg hm~(–2)处理的籽粒产量最高,比秸秆不还田和施氮量225 kg hm~(–2)处理(CK)2年平均增产6.33%,增产的主要原因是百粒重和行粒数的显著提高和秃尖的显著降低;玉米籽粒产量并未随着施氮量的增加而持续增加;相同施氮量条件下,秸秆还田比秸秆不还田2年平均群体生物产量增加2.95%。秸秆还田配施氮肥能够增加春玉米株高、茎粗、叶面积,提高叶绿素含量和光合作用,相同施氮量条件下,秸秆还田比秸秆不还田处理2年平均灌浆期叶面积增加2.71%,光合速率提高4.80%。综合分析认为,秸秆还田9000 kg hm~(–2)和配施氮肥225kg hm~(–2)是辽北棕壤区春玉米生产比较理想的还田和施肥模式,在该区域农业发展中具有一定的应用价值。     

氮素实时管理对冬小麦产量和氮素利用的影响

为实现氮素效率和小麦产量的协同提高,以山东省泰安市和兖州市为试验地点,连续2年在4个田块上进行了基于土壤硝态氮测试的氮素实时管理试验。与农民习惯施肥相比,优化施氮处理提高产量0.87%～10.44%,平均5.82%;而氮肥用量减少38.61%～53.29%,平均46.70%;氮素吸收效率、氮素表观利用率和氮素农学效率分别增加36.67%～85.69%、58.49%～267.69%和34.16%～410.58%;氮肥偏生产力升高74.23%～124.87%;产/投比提高78.50%～112.09%。说明应用土壤硝态氮测试进行小麦氮肥实时实地管理达到了减少氮肥用量,提高氮素利用效率,增加产量和经济效益的目的。     

不同氮肥水平下早熟晚粳氮和磷的吸收利用特性及相互关系

采用大田试验,以长江中下游地区具有代表性的50个早熟晚粳品种为材料,研究7个氮肥水平(0、150.0、187.5、225.0、262.5、300.0、337.5 kg hm^-2纯氮)下水稻氮和磷积累量、吸收速率、利用效率的差异及其相互关系。结果表明：(1)在0~337.5 kg hm^-2纯氮范围内,随着氮肥水平的增加,早熟晚粳的植株含氮率和氮积累量在拔节、抽穗和成熟期均显著增加;植株含磷率和磷积累量在拔节和抽穗期显著增加,成熟期呈先增后减变化。(2)播种至拔节阶段氮和磷吸收速率随施氮量的增加而提高,差异极显著;拔节至抽穗阶段氮和磷吸收速率随施氮量的增加呈现先增加后降低的变化趋势;抽穗至成熟阶段的氮和磷吸收速率规律不明显。(3)在0~337.5 kg hm^-2纯氮范围内,随着施氮量的增加氮素籽粒生产效率和磷素籽粒生产效率均显著降低,随着施氮量的增加基因型之间的差异减小;随着施氮量的增加氮和磷收获指数都呈现抛物线关系,在施氮量为262.0 kg hm^-2纯氮时出现最大值。(4)早熟晚粳对氮和磷的吸收利用具有显著的协同效应,但随生育进程的推进这种效应减弱。水稻在播种至拔节、拔节至抽穗和抽穗至成熟3个生育阶段的氮和磷吸收速率都呈二次曲线关系(r=0.892^**,r=0.736^**,r=0.512^**)。(5)相关分析表明,产量与拔节期、抽穗期和成熟期的吸氮量和吸磷量以及播种至拔节期和拔节至成熟期的吸氮速率和吸磷速率呈极显著正相关关系。增施氮肥有利于水稻氮和磷吸收利用的提高,但氮肥过高时氮和磷吸收利用不再增加,甚至有所降低。     

基肥配比和拔节期追氮对糯玉米淀粉胶凝和回生特性的影响

以苏玉糯4号为材料,采用二因素裂区设计,研究了不同基肥配比(纯N 75 kg hm^-2、纯N 75 kg hm^-2+K₂O 70 kg hm^-2、纯N 75 kg hm^-2+P₂O₅ 65 kg hm^-2和纯N 75 kg hm^-2 +P₂O₅ 65 kg hm^-2+K₂O 70 kg hm^-2)和拔节期追氮(0、150和300 kg hm^-2)对糯玉米淀粉胶凝和回生特性的影响。结果表明,淀粉和回生淀粉的起始温度、峰值温度和终值温度虽然受到施肥处理的影响,但总体上变异较小。热焓值受基肥配比影响较小,糊化范围和峰值指数受拔节期追氮量影响较小。在不同基肥配比处理下,糊化范围在基施氮钾时最低,氮磷钾合理配施处理下最高,峰值指数表现和糊化范围相反。在拔节期不同追氮量处理下,热焓值以追氮150 kg hm^-2时最高,追氮300 kg hm^-2或不追氮无显著差异。胶凝淀粉冷藏后发生回生,表现为转变温度、热焓值和峰值指数降低,糊化范围变宽。和仅基施氮相比,增施磷或(和)钾都可降低淀粉的回生值和回生淀粉的热焓值,拔节期追氮处理的这两项指标均劣于不追氮处理。回生值与回生淀粉的热焓值和峰值指数显著正相关,相关系数分别为0.90 (P < 0.01)和0.41 (P < 0.05); 原淀粉的热焓值与峰值指数极显著正相关, 相关系数为0.65 (P < 0.01),与回生淀粉热焓值显著正相关,相关系数0.44 (P < 0.05),与终值温度显著负相关,相关系数-0.41 (P < 0.05)。在本试验条件下,以氮磷钾均衡基施并拔节期追氮150 kg hm^-2时,淀粉胶凝和回生特性较为理想,表现为热焓值较高,回生值较低。