Sex Pheromone Traps for Monitoring the European Vine Moth,Lobesia botrana: Effect of Dispenser Type,Pheromone Dose,Field Aging of Dispenser,and Type of Trap on Male Captures

The effects of dispenser type, pheromone dose in the dispenser, aging of dispenser in the field, and trap type on trapping efficiency of males of the European vine moth,Lobesia botrana Den. et Schiff. (Lepidoptera: Tortricidae), were investigated. LB Pherocon caps and locally manufactured septa were equally effective in attracting males to traps. Within the range of 0.1 to 100 μg pheromone/dispenser, response of males increased positively with the pheromone dose. Within the first 7 days tested, pheromone loads of 1 or 10 mg/dispenser were significantly less attractive to males than 0.1 mg/dispenser. The effect of aging of the dispenser (loaded with 1 mg pheromone) in the field on trapping efficiency was significant. Captures in traps baited with 4- and 5-week-old septa were significantly lower (by 24%) than those in traps baited with 1-week-old septa. The fairly slow loss of attractancy exhibited by rubber septa indicates that septa may be kept in traps for at least 5 weeks. The release rate of the pheromone from the Israeli dispensers (1 mg loaded) was measured in a flow system. The emission of the pheromone decreased gradually as a function of age, correlating well with the lower trap catches of field-aged septa. Release rates were reduced by 56%, 70% and 84% after 4, 5 and 6 weeks, repectively. The nonsticky IPS trap was as effective in capturing the European vine moth males as the sticky Pherocon 1C trap. The possibility of using the non-sticky, nonsaturating and easy-to-handle IPS traps may lead to better and easier monitoring of the European vine moth populations.     

Population dynamics and biological control of European grapevine moth (Lobesia botrana: Lepidoptera: Tortricidae) in Albania using different strains of Bacillus thuringiensis

This work describes the results of an experiment conducted in a vineyard in Durres, Albania from 2004 to 2006, to study the efficacy of two strains of Bacillus thuringiensis Berliner, vars kurstaki and aizawai, against the moth Lobesia botrana Denis & Schiffermüleler (Lepidoptera: Tortricidae), a major pest of grapevines. The experimental results revealed that the two strains were significantly more effective than the control, with var. aizawai being more effective than var. kurstaki. Bacillus thuringiensis (Bt) provided better protection of the bunches from the second generation compared to the third one showing the short persistence of action of the active ingredient. The high degree of efficacy supported a role for Bt in grapevine moth management. Our study also provided insights into the population dynamics of the European grapevine moth under Albanian climatic conditions. Despite the necessity for the pheromone traps to indicate the start of the flight period, the experiments confirmed the lack of a correlation between the population dynamics and the level of damage by the pest. Pheromone trapping can offer very useful indications regarding the start of the flight period of each generation and its duration but not on the severity and incidence of the pest.     

Control of the pink bollworm <Emphasis Type="Italic">Pectinophora gossypiella</Emphasis> by the mating disruption technique on cotton in a semi-arid region of Turkey

This study evaluated the effectiveness of mating disruption pheromones for the pink bollworm (PBW) Pectinophora gossypiella (Saund.) (Lep.: Gelechiidae) and determined the damage levels of cotton plants in Turkey in 2007 and 2008. Adult populations were monitored by pheromone traps, with 250 pieces ha⁻¹ of PBW rope dispensers used for each treated field of approximately 12 ha. Calculations were based on comparing cotton yield in the control field with that in treated fields. The PB-Rope L dispensers significantly decreased both number of males in the pheromone traps and cotton boll damage. Results showed that PB-Rope L dispenser application during the square period of cotton plants effectively reduced damage. Cotton yield of treated fields was increased compared with control fields by 20.28% and 10.28% in 2007 and 2008, respectively. Infestation ratios were determined by comparing cotton bolls collected from treated fields with bolls from control fields; the results were 25% and 15% for control fields and 16% and 4% for treated fields in 2007 and 2008, respectively. Larval densities in the control fields were higher than those in the treated fields in both years. Results showed that the PB-Rope L dispensers released pheromone satisfactorily to disrupt pest mating.     

不同果园中主要食心虫种群监测与防控技术

利用性信息素和黑光灯系统调查了枣园、桃园中食心虫的种类构成及其种群发生动态,并比较了3种土壤防控措施对桃小食心虫的效果。结果显示：枣园中食心虫的数量由多到少依次为：桃小食心虫、梨小食心虫和桃蛀螟,其中桃小食心虫1年发生2代,发生期为6月上旬至9月下旬,其成虫累积诱捕量随日期的变化符合Sigmoid方程;梨小食心虫1年发生4代,发生期为5月下旬至9月下旬;桃蛀螟1年发生2代,发生期为5月下旬至9月下旬。桃园中以梨小食心虫为主,1年发生4～5代,发生期为5月上旬至9月底,年均性信息素累积诱捕量为8829头/诱捕器,而桃小食心虫和桃蛀螟数量均极少。3种土壤防控措施中,灌水覆膜对桃小食心虫防控效果最好,幼虫出土抑制率为67%,其次为盖草覆膜和盖土拍实。     

Pest management programmes in vineyards using male mating disruption

Israeli vine growers have been reluctant to adopt the mating disruption technique for control of the European vine moth, Lobesia botrana Den. & Schiff. Since the chemically controlled honeydew moth, Cryptoblabes gnidiella Mill., coexists with the European vine moth, growers have maintained that the use of mating disruption would fail to bring about a significant reduction in pesticide use. In this study, the efficacy of mating disruption techniques against C. gnidiella was tested, as well as the effect of these methods on pesticide use and damage to clusters when the method was employed against both of the pests in wine grapes. Comparisons were made between plots treated with (1) L. botrana mating disruption pheromone, (2) L. botrana and C. gnidiella mating disruption pheromones and (3) control plots. A significant difference in the number of clusters infested with the developmental stages of the moths was seen between pheromone-treated plots and controls, while no such difference was observed between plots treated with one versus two pheromones. A similar pattern was observed in the number of insecticide applications; the greatest number of applications was used in control plots, followed by plots treated with L. botrana mating disruption pheromone and by plots treated with pheromones against both pests, in which no pesticides were applied.     

Egg and larval parasitoids of the beet armyworm<Emphasis Type="Italic">Spodoptera exigua</Emphasis> on maize in Turkey

The present study was conducted to determine egg and larval parasitoids of the beet armywormSpodoptera exigua Hübner (Lepidoptera: Noctuidae), which is an important but sporadic pest in Turkey. High beet armyworm population levels were recently observed in fields of first and second crop maize in the southeast Mediterranean region of Turkey. The parasitoid species complex and its impact on the pest were analyzed in a 4-year study in first and second crop maize. The braconid larval parasitoidsMicroplitis rufiventris Kokujev,M. tuberculifer Wesmael,Meteorus ictericus Nees,Chelonus obscuratus (Herrich Schäffer) (an egg-larval parasitoid),Apanteles ruficrus (Haliday); the ichneumonid larval parasitoidsHyposoter didymator (Thunberg) andSinophorus xanthostomus Gravenhorst; and the egg parasitoidTrichogramma evanescens (Westwood) were found to be the natural enemies attacking the pest. Among the parasitoid species the solitary endoparasitoidH. didymator was the most prevalent species, being reared from 40.5% of the parasitized larvae found. Higher parasitism rates were recorded on first crop than on second crop maize in every year. Possible reasons for this difference in larval parasitism between two growing seasons include lower population of the pest and reduced insecticide applications in first crop maize fields which permitted higher parasitism. However, parasitoid activity was insufficient to counterbalance the population growth of the pest on subsequent second crop maize.     

Control of the codling moth (cydia pomonella) in apple and pear orchards in Israel by mating disruption

Control of the codling moth,Cydia pomonella (L.) (Lepidoptera: Tortricidae), by pheromone-mediated mating disruption, was studied in Israel using two types of dispensers: Shin-Etsu twist-tie (normal and long-life) and TNO matrix formulation (square wafers). Factors affecting mating disruption: initial pest population density, timing of the pheromone application, early season insecticide treatments and monitoring of the pest, were evaluated. Efficacy of mating disruption was assessed by comparing catch of males in pheromone traps located in pheromone-treated and chemically sprayed control plots. Fruit infestation during the season and at picking time was the major criterion to assess mating disruption. The preferred time for pheromone application was before the first flight of the moth. When pheromones were applied at a later stage, one or two early insecticide sprays had to be used to reduce the initial level of the codling moth population. In all tests, trap catch in pheromone-treated plots was drastically reduced as compared with respective control plots. In pheromone-treated plots where total seasonal trap catch (after pheromone application) was less than 12 males/trap, fruit infestation was less than 1.5%; zero trap catch resulted in 0.1-0.3% infestation. At high population levels, mating disruption was ineffective, with the rate of infestation at picking-time being similar in pheromone-treated and control plots. All the field tests indicated that mating disruption with either Shin-Etsu ropes or TNO squares can control codling moth infestations in apple and pear orchards, at relatively low densities of the pest, as efficiently as commercial insecticide sprays.     

Efficacy of mating disruption technique against carob moth,Apomyelois ceratoniae Zeller (Lepidoptera: Pyralidae) in pomegranate orchards in Southeast Turkey (Şanlıurfa)

Carob moth, Apomyelois (=Ectomyelois) ceratoniae Zeller (Lepidoptera: Pyralidae) is a key pest in pomegranate orchards in Southeast of Turkey. The pest causes significant damage and reduces marketability of fruits, and is difficult to control with insecticides. Therefore, it is imperative to develop environmentally sound effective alternative management approaches. In this study, we aimed to evaluate the mating disruption technique and determine the appropriate application dose for the management of the pest. The study was carried out in Central and Suruç districts of ?anl?urfa province (Turkey) in 2011 and 2012. SPLAT EC (Specialized Pheromone-Lure Application Technology) trade named dispenser containing 2% Z7, E9-11-dodecatrienyl formate pheromone mimic was applied at 500, 625 and 750 g/ha in three pomegranate orchards in each district. Results indicated that mating disruption technique significantly reduced the pest populations. But effectiveness of the method was less in terms of the damage rate on fruits. Pheromone applied at 500 g/ha was not effective. However, application at 625 g/ha reduced the pest populations to an acceptable level. Thus, the concentration could be considered an appropriate dose for the less abundant pest population within integrated pest management while higher population would require combining the mating disruption method with a bio-insecticide.     

Influence of the carpophagous generations of the European grapevine moth Lobesia botrana on grape bunch rots

The second and third generations of Lobesia botrana can cause both yield losses and spreading of grape bunch rots. The aim of this study was to know to what degree these generations of L. botrana influence the rots at harvest time. The study was carried out in two grape-growing areas of northeastern Italy where the moth completes two and three generations per year, respectively. In the context of 12 insecticide trials, samplings were conducted in different plots to estimate the number of larval nests of the second and third generations and that of rotten berries at harvest time. The data sets (number of larval nests and rotten berries) from the different trials were submitted to regression analyses. Gray mold was the most important grape rot. The second generation, in the area where L. botrana has only two generations per year, and the third generation, in the area where the moth completes three generations per year, can favor gray mold on and around berries bored by larvae. In contrast, groups of rotten berries non-contiguous to larval nests did not appear to be influenced by moth activity. In the area where the moth completes three generations per year the larval nests of the second generation did not show a significant influence on bunch rots at harvest time.     

Evaluating sex-pheromone- and kairomone-based lures for attracting codling moth adults in mating disruption versus conventionally managed apple orchards in Pennsylvania

BACKGROUND: Effectiveness of different types of commercial sex‐pheromone‐ and kairomone‐based lures for attracting codling moth adults may vary under different pest management practices. The attractiveness of four types of codling moth (CM) lures (CM L2 Long‐Life^®, CM 10X Megalure^®, Pherocon CM DA^® and Pherocon CM‐DA Combo^®) was evaluated in commercial apple orchards either treated with sex pheromone mating disruption (MD) or only conventional insecticides (non‐MD) in Adams County, Pennsylvania, in 2006 and 2007. RESULTS: CM DA Combo lure was most effective in terms of mean seasonal weekly moth capture as well as mean cumulative moth capture in MD orchards. In both years, the CM L2 lure was as attractive to adult moths as the CM DA Combo lure in non‐MD orchards. The CM DA and CM 10X lures caught significantly fewer moths in both MD and non‐MD orchards compared with the CM DA Combo lure. CONCLUSIONS: ON the basis of mean seasonal weekly moth capture as well as mean cumulative moth capture, the CM DA Combo and CM L2 lures were found to be significantly more effective for monitoring CM adults in both MD and non‐MD orchards. In contrast, the CM DA and CM 10X lures were not as effective in either type of orchard. Copyright © 2011 Society of Chemical Industry     

小菜蛾性信息素研究及应用进展

小菜蛾是十字花科蔬菜重要害虫 ,抗药性发展很快而难以防治 ,应用性信息素是综合治理小菜蛾的重要手段。本文对小菜蛾性信息素的鉴定、性信息素的分泌部位及感受部位、人工合成性信息素方法和应用技术 ,以及小菜蛾性信息素应用时所受影响因子进行了综述和讨论     

Plant volatile compounds shorten reaction time and enhance attraction of the codling moth (Cydia pomonella) to codlemone

BACKGROUND: The codling moth is the most serious pest of deciduous tree fruit (apples, pears, crabapples, walnuts, quince) worldwide. The high frequency of insecticide treatments per season has resulted in breakdown of codling moth control owing to insecticide resistance. As an alternative, integrated pest management includes mating disruption to achieve population suppression in orchards. Under this scheme, the sex pheromone of the codling moth, (E, E)‐8,10‐dodecadien‐1‐ol (codlemone), is released from dispensers in crops to hinder mating by luring males. Increasing the attractiveness of codlemone formulations to codling moth males can be regarded as a key to increasing the efficacy of mating disruption. With this aim, the effects of adding plant volatiles on the behavioural responses of codling moth males to codlemone were tested. RESULTS: Adding R(+)‐limonene, linalool, (E)‐β‐farnesene or ethyl (E, Z)‐2,4‐decadienoate to codlemone significantly increases the proportion of males flying to the pheromone source in a wind tunnel. The response level is equivalent to that of males responding to females releasing codlemone. Using real‐time recordings, it is shown how these four plant products also shorten the response time of males to codlemone under the behavioural criteria time to activation, time till upwind flight is induced and time to pheromone source contact. CONCLUSION: Shortening the response time and increasing source location by males of dispensers releasing codlemone with R(+)‐limonene, linalool, (E)‐β‐farnesene or ethyl (E, Z)‐2,4‐decadienoate added would enhance mating disruption through better engagement of males with dispensers, to the detriment of females. Copyright © 2011 Society of Chemical Industry     

The parasitoid complex ofLiriomyza cicerina on chickpea (Cicer arietinum)

Liriomyza cicerina (Diptera: Agromyzidae) is an important pest on chickpea in Turkey. The objective of this study was to determine the parasitoids and rates of parasitism ofL. cicerina on chickpea (Cicer arietinum L.) during the 2005 and 2006 seasons in ?anl?urfa province, Turkey. Leaves with mines were sampled weekly and kept in the laboratory to observe and count emerging leafminer and parasitoid adults. Eight parasitoid species were collected: the braconidsOpius monilicornis Fischer andOpius tersus Foerster and the eulophidsDiaulinopsis arenaria (Erdös) andNeochrysocharis formosa (Westwood), which occurred in both the winter and summer seasons;Diglyphus crassinervis Erdös,Neochrysocharis ambitiosa Hansson,Neochrysocharis sericea (Erdös) andPediobius metallicus (Nees), which occurred only in the summer growing areas.Diaulinopsis arenaria was the predominant parasitoid with 4–7.7% parasitism rate whileN. ambitiosa andO. monilicornis were the second and third most predominant species. The results of these trials show that sinceDia. arenaria occurred throughout every season, it could potentially be used for control of the leafminerL. cicerina.     

Pheromone Research and Development in Israel 1975–2015

The review describes the history of pheromone research in Israel in 1975–2015. The research focused on sex pheromones of moths that were important agricultural pests. Identification, synthesis and field application of sex pheromones was performed. Synthetic procedures of several known sex pheromones were developed. Monitoring and control of key pest moths was evaluated. The interactions of pheromone components of closely related species were studied in field and laboratory experiments. The sex pheromones of three scale insects, two mealybug species and Matsucoccus josephi were studied. New syntheses were developed and the pheromones were implemented in pest management. Structure activity relationship of the pheromonal and kairomonal of the M. josephi pheromone was investigated. Different pherotypes of P. ficus were identified and evaluated. The aggregation pheromone of sap beetles in combination with food baits was evaluated. The aggregation pheromone of the almond bark beetle was identified and a stereospecific synthesis of its enantiomers was developed. Monitoring the pest in stone fruit orchards was implemented. The activity of the pheromone biosynthesis activating neuropeptide (PBAN) was studied in Helicoverpa armigera and Heliothis peltigera. The ligation technique was used to assess the effect of PBAN on the production of female and male pheromones. A structure-activity relationship study of PBAN indicated that shorter peptides display activity as the full length PBAN. A series of linear and cyclic peptide analogs was prepared, resulting in the discovery of a lead antagonist. The research and development activity facilitated the intensive integration of pheromones in the pest management regimes in Israeli agriculture.     

Phenology of the potato tuber moth (phthorimaea operculella), a pest of potatoes and of processing tomatoes in israel

The potato tuber moth (PTM),Phthorimaea operculella (Zeller), is a pest of solanaceous plants. In Israel it damages mainly potatoes, and tomatoes grown for processing. The PTM favors high temperatures and drought. Under these conditions its development period is short, resulting in overlapping generations and high populations in mid-summer. A phenological study, based on the use of the female sex pheromone, showed that moth flight occurred throughout the year. Large populations of the PTM were present only during the hot season. Populations were highest in the semi-arid Negev, where potatoes are grown on a large scale during two seasons of the year; in other areas, PTM populations were much lower.     

Targeting Cydia pomonella (L.) (Lepidoptera: Tortricidae) adults with low‐volume applications of insecticides alone and in combination with sex pheromone

BACKGROUND: Studies surveyed the toxicity of several insecticides against adult codling moth, Cydia pomonella (L.), and examined the field effectiveness of applying low‐volume (12 L ha^?1) sprays alone or in combination with a microencapsulated (MEC) sex pheromone formulation. RESULTS: Neonicotinyls, organophosphates and synthetic pyrethroids significantly reduced fecundity at concentrations nearly 100‐fold lower than their maximum labeled field rate. Field studies in 2005 demonstrated that six applications of esfenvalerate resulted in > 90% reduction in fruit injury versus the untreated check. The addition of the MEC pheromone formulation did not further improve control. Five sprays of esfenvalerate, phosmet and acetamiprid all significantly reduced levels of fruit injury compared with the untreated control in 2006. Esfenvalerate and acetamiprid mixed with the MEC pheromone significantly reduced fruit injury compared with the MEC‐only treatment. Significant increases in pest and decreases in predator mite densities occurred in plots treated with esfenvalerate in both years. Low‐volume sprays of phosmet and acetamiprid did not disrupt mites. CONCLUSION: Low‐volume insecticide sprays can effectively manage codling moth and are less disruptive of integrated mite management. Developing an effective ‘attract and kill’ technology with this approach will require optimization of the attractant(s) to maximize moth exposure to insecticide residues. Published 2010 by John Wiley & Sons, Ltd.     

苹果蠹蛾不同防治方法的控害效应比较

为了及时地在苹果蠹蛾Cydia pomonella(L.)入侵地实施有效的防控策略,通过评价活雌蛾诱捕器诱捕雄蛾数量、在树干上化蛹或越冬的幼虫数量以及对苹果果实的为害程度,比较性信息素诱杀技术、迷向技术和化学防治技术对苹果蠹蛾的控害效应。结果显示,采用迷向法控制苹果蠹蛾的防治效果与化学防治相当,其中在第1代幼虫为害盛末期,蛀果率可控制在1%以下,在第2代幼虫为害盛末期,可控制在2%左右;而诱捕区与对照区(无处理)的蛀果率相当,几乎无控制效应。     

Catching Ips duplicatus (Sahlberg) (Coleoptera: Scolytidae) with pheromone‐baited traps: optimal trap type,colour, height and distance to infestation

BACKGROUND: Field trapping experiments were carried out to evaluate effective trap characteristics for maximising Ips duplicatus (Sahlberg) catches in pheromone‐baited traps in China. RESULTS: Window‐slot and cross‐barrier traps had significantly higher catches than multiple‐funnel traps. The colour of window‐slot traps showed a significant effect on catches, with dark colours (black and red) being more effective than light colours, especially white and yellow. Window‐slot traps at a 1.5–2.0 m level caught more beetles than those at either ground level (0–0.5 m) or at 3.5–4.0 m. Ips duplicatus can be attracted to pheromone‐baited traps over a distance of > 100 m from the forest edge in an open grassy field. There was a strong diurnal pattern of flight activity, with catches on window‐slot traps occurring during the daytime with one broad peak at mid‐ to late afternoon. The seasonal flight activity of I. duplicatus as monitored by pheromone‐baited window‐slot traps during 2007–2008 indicated that three major flight peaks occurred in early June, late June–early July and late July respectively, suggesting the existence of a potential second generation. CONCLUSION: The optimal trap characteristics will improve the performance of pheromone‐baited traps as a critical monitoring or mass‐trapping tool to combat outbreaks of this pest species. Copyright © 2009 Society of Chemical Industry     

Developing Pheromone Traps and Lures for Maruca vitrata in Taiwan

The larvae of the legume pod borer, Maruca vitrata (Lepidoptera: Pyralidae), cause severe damage on economically important legume crops in the tropics. The female moth produces volatile components to attract males for mating. The so-called sex pheromones are species-specific multi-component blends and are used as lures in crop protection for pest monitoring. Their chemical identification and ratios is critical to design efficient lures. The following sex pheromone components for M. vitrata have been described: (E, E)-10,12-hexadecadienal (major compound), (E, E)-10,12-hexadecadienol and (E)-10-hexadecenal (minor components). The ratio of 100:5:5 of these components was the most attractive in trapping experiments in Benin, Africa. According to this ratio, a synthetic pheromone lure was developed for commercial use. But the commercially available blend was not attractive in field trapping experiments in other regions of sub-Saharan Africa and Southeast Asia. These findings lead to the conclusion that there is a possible polymorphism in the blend composition of the M. vitrata sex pheromone among populations from different geographical regions. In Taiwan, M. vitrata moths were never caught efficiently by the commercially available pheromone lures and traps. This paper reports trap and lure optimization experiments for effective trapping of Taiwanese M. vitrata moths in different leguminous crops.     

Phyllonorycter apparella, a new record and a new pest of trembling aspen (Populus tremula) in Turkey

Phyllonorycter apparella (Herrich-Schäffer, 1855) (Lepidoptera: Gracillariidae) is a new record for the Turkish fauna and a new trembling aspen(Populus tremula L.) pest for Turkey. Its biology, damage and infestation ratio were studied in Sankami?. (Kars), Turkey, during 1996 and 1997.P. apparella has one generation a year. The developmental stages of the pest are described briefly. P.apparella hibernates in the adult stage. At the beginning of June, the adults appear. Females deposit their eggs on both leaf surfaces, generally one by one, 7–10 days after emerging. The early instars (sap-feeding larvae) start to mine mostly from the underside of leaves. Each mine has one larva, and each leaf may have up to 26 mines. The last instar (tissue-feeding larva) begins to pupate in the mine after mid August. The adults emerge from mid September to the beginning of October, and move to the trunks of old pine trees, where they hibernate in bark crevices. The infestation level may be as high as 90% of the leaves on some trees.