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长期施肥对酸性土壤氨氧化微生物群落的影响 总被引:1,自引:2,他引:1
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研究主要分析氮元素对宁夏平罗盐渍化枸杞园土壤中氨氧化微生物(氨氧化细菌和氨氧化古菌)的影响。实验共设八个处理:(1)C(不施氮肥,不施脱硫废弃物,原始荒地);(2)不施氮肥(N0);(3)施氮肥25 kg hm~(-2)(N25);(4)施氮肥50 kg hm~(-2)(N50);(5)施氮肥100kg hm~(-2)(N100);(6)施氮肥200 kg hm~(-2)(N200);(7)施氮肥400 kg hm~(-2)(N400);(8)施氮肥800 kg hm~(-2)(N800),在N0-N800处理施用脱硫废弃物3.72×104kg hm~(-2)。2011年8月采集0~20 cm土样。结果显示:脱硫废弃物和氮肥配合施加对土壤理化性质产生了显著影响;NO_3~--N和NH_4~+-N含量在施氮处理中相对于原始样地和不施氮处理组都有显著的升高,微生物生物量和细菌和氨氧化细菌多样性指数在施氮400 kg hm~(-2)达到最大值,施氮肥400 kg hm~(-2)是促进微生物量和群落多样性增加的最佳施用量。实时荧光定量PCR结果显示:氨氧化细菌(AOB)丰度在施氮肥400 kg hm~(-2)和800 kg hm~(-2)显著高于其它处理,脱硫废弃物和氮肥配合施用对AOB的丰度具有叠加效应。相关性分析表明:NH_4~+-N与总磷脂脂肪酸(PLFA)、细菌PLFA、革兰氏阳性菌(G+)、革兰氏阴性菌(G-)、真菌/细菌(F/B)、微生物碳(MBC)、微生物氮(MBN)及16S r RNA基因拷贝数、AOB的基因拷贝数都显著相关。因此,铵态氮是该地区微生物群落可利用的有效氮素。 相似文献
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土地利用方式对万木林土壤氨氧化微生物丰度的影响 总被引:2,自引:1,他引:2
以我国亚热带地区典型花岗岩发育酸性红壤为研究对象,选取福建建瓯万木林自然保护区封禁保护下5种自然植被和1种人工种植植被土壤,采用荧光实时定量PCR(Real-time PCR)技术测定了土壤氨氧化细菌(AOB)和氨氧化古菌(AOA)的群落丰度,采用15N稳定同位素成对标记和数值模型相结合的方法测定了土壤初级硝化速率。结果显示,长期封禁保护下的自然植被土壤pH低,土壤AOB数量偏低。人为种植和管理显著提高了土壤pH,促进了AOB的生长,其丰度比自然条件下提高了2个数量级,土壤初级硝化速率也显著提高,并与AOB数量存在显著的相关性,表明AOB是硝化作用的主要贡献者。5种自然植被条件下AOA的amoA基因拷贝数占泉古菌16S rRNA基因的比例都小于1%(0.01%~0.64%),在农业利用方式下上升到5.32%,表明并非所有泉古菌都具备氨氧化功能基因amoA,氮肥施用可能促进了氨氧化古菌的生长。 相似文献
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不同生育期苹果园土壤氨氧化微生物丰度研究 总被引:1,自引:1,他引:1
【目的】大量施氮引起的土壤酸化问题已严重制约苹果的安全生产。氨氧化微生物驱动的土壤硝化作用是土壤氮素循环的重要环节,探析苹果园土壤中氨氧化微生物氨单加氧酶基因(amoA)丰度与土壤硝化强度(Potential Nitrification,PN)和土壤理化性质的相关性,有助于评价土壤氨氧化微生物类群在苹果园土壤生态系统中的作用。【方法】本研究以辽宁省丹东东港地区‘寒富’苹果园土壤为研究对象,采用实时荧光定量PCR(Real-time PCR)技术,检测苹果树不同生育时期, 4月28日(萌芽期)、 7月24日(新梢停长期)、 10月23日(落叶期)的两个苹果园(分别编号为D1和D2)的土壤理化性质、 土壤硝化强度、 氨氧化古菌(Ammonia-oxidizing archaea, AOA)和氨氧化细菌(Ammonia-oxidizing bacteria, AOB)的amoA基因丰度,并分析了氨氧化微生物丰度与土壤理化性质和土壤硝化强度之间的关系。【结果】不同生育时期‘寒富’苹果园土壤理化因子差异较大。所有供试土壤的硝态氮(NO-3-N)、 速效磷(AP)和速效钾(AK)含量在4月份最高,铵态氮(NH+4-N)含量在7月最高,NO-3-N、 NH+4-N、 AP和AK含量在10月多为最低。且所有供试土壤pH值均在4.25~6.09之间。同一生育时期内,D2土壤pH均显著高于D1土壤,但其NO-3-N和NH+4-N含量则不同程度地低于D1土壤。不同采样时期的果园土壤硝化强度随季节变化表现出先降后增的趋势,除7月D2土壤硝化强度显著高于D1土壤外,4月与10月D2土壤硝化强度均显著低于D1土壤。尽管不同采样时期的土壤AOA与AOB丰度随生育期而各异,所有供试土壤中AOA丰度均显著高于AOB丰度。同一时期内,D2土壤AOA和AOB丰度均显著高于D1土壤。尽管土壤pH、 NO-3-N与AOA、 AOB均表现出显著相关性,土壤PN仅与AOA丰度明显正相关。【结论】长期施肥导致苹果园土壤pH值降低,pH值的改变是影响AOA与AOB丰度的重要因子,果园土壤的硝化过程主要由AOA来完成,土壤硝化强度与季节变化引起的温度和土壤环境因子等的改变密切相关。苹果园无机氮肥混合有机肥的施入,同时结合自然生草、 人工刈割等管理制度,在一定程度上可改变土壤氮素的含量与种类,减缓土壤酸化。 相似文献
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长期施肥对棕壤氨氧化细菌和古菌丰度的影响 总被引:6,自引:1,他引:6
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施肥与覆膜等农田管理措施能够改变土壤的物理化学性质,这直接影响着驱动氨氧化过程的氨氧化微生物,而氨氧化过程是硝化作用的限速步骤。以沈阳农业大学棕壤长期施肥与覆膜试验站为平台,采用荧光定量PCR技术,研究了5种施肥制度下[不施肥(CK)、氮肥(N)、氮磷肥(NP)、有机肥(M)和有机无机配施(MNP)]土壤细菌、泉古菌和氨氧化微生物数量的变化。结果表明,不同施肥处理细菌、泉古菌、氨氧化细菌和氨氧化古菌的基因拷贝数平均值分别为0.52×109~4.20×109、2.14×108~9.69×108、0.21×107~6.89×107和0.26×107~74.70×107copies g-1干土。与CK相比,有机肥处理(M、MNP)能显著增加土壤细菌的丰度,化肥处理(N、NP)则相反;施肥尤其是化肥处理(N、NP)均能降低泉古菌和氨氧化古菌的丰度;有机肥处理(M、MNP)显著增加了氨氧化细菌的丰度。细菌、泉古菌、氨氧化细菌和氨氧化古菌丰度均与pH值存在显著正相关关系(P0.05),细菌和氨氧化细菌丰度则主要受全碳含量的影响,而细菌、泉古菌和氨氧化细菌丰度与铵态氮、硝态氮含量存在极显著负相关关系(P0.01)。研究结果可为进一步探讨农田生态系统中氨氧化微生物对不同管理措施的响应机制及其在氮素转化中的作用提供科学依据。 相似文献
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采用基于氨单加氧酶基因的PCR-RFLP和DNA测序技术,以黄土高原旱地黑垆土为材料,研究长期施用氮肥和磷肥对土壤氨氧化古菌多样性的影响。结果显示,不同施肥处理土壤样品得到的氨氧化古菌的OTU数分别为25(种植不施肥,CK)、21(不施肥不种植,LD)、18(单施氮肥,N)、25(单施磷肥,P)和13(氮、磷共施,NP)。氨氧化古菌的多样性指数H′和优势度指数Ds变化趋势基本相同,分别为P〉CK〉LD〉NP〉N和P〉CK〉NP〉LD〉N;种群丰富度和均匀度指数在不同处理间变化较大,分别为CK〉P〉LD〉N〉NP和P〉NP〉LD〉N〉CK。各处理优势氨氧化古菌绝大部分属于Cluster S,少数属于Cluster M,获得的序列全部属于难培养泉古菌门。不同施肥方式的长期定位试验土壤中氨氧化古菌多样性变化较大,而优势氨氧化古菌系统进化定位没有显著变化。 相似文献
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长期施用化肥和有机肥显著改变了我国土壤肥力和农田生态系统的稳定性。微生物在土壤养分循环和肥力形成过程中扮演了重要角色,其数量、多样性及群落结构是评价土壤肥力和农田生态系统稳定性的重要指标。随着研究土壤微生物的方法和技术的成熟,当前关于如何选择微生物学参数以有效评价土壤肥力及健康状况、土壤微生物对不同施肥管理措施的响应机制及功能微生物如何调控土壤养分循环等问题已成为土壤生态学中的研究热点。本文梳理了基于我国长期定位施肥农田的土壤微生物学研究进展,综合分析了土壤微生物生物量和酶活性、细菌群落及近些年倍受关注的氨氧化菌群落三方面的研究进展及发展方向,其中综合分析了构成细菌群落的八大门类菌群对施肥的响应及其驱动因素,最后从不同角度提出了微生物在土壤学及农业生产中的研究和应用方向。 相似文献
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Yi-Fei Sun Ju-Pei Shen Cui-Jing Zhang Li-Mei Zhang Wen-Ming Bai Ying Fang Ji-Zheng He 《Journal of Soils and Sediments》2018,18(3):762-774
Purpose
Changes of nitrogen (N) cycle caused by N fertilization and precipitation regimes have affected the key ecosystem structure and functions in temperate steppe, which may modify the structure of soil microbial communities involved in N transformation. This paper was designated to examine the response of soil ammonia oxidizers and denitrifiers to the N fertilization and precipitation regimes in a semi-arid steppe where N and water contents are major limiting factors of the grassland productivity.Materials and methods
This study was based on a long-term N fertilization and precipitation regimes experiment in Inner Mongolia (116° 17′ 20″ E, 42° 2′ 29″ N). The treatments including CK (control), R (reduced precipitation), W (30% increase in precipitation), N (10 g N m?2 y?1), RN (reduced precipitation and 10 g N m?2 y?1), and WN (30% increase in precipitation and 10 g N m?2 y?1). Soil basic chemical properties and microbial activities were analyzed. Molecular methods were applied to determine the abundance, structure and diversity of ammonia oxidizers and denitrifiers. Statistical analysis detected the main and interactive effect of treatments on soil microbial communities and revealed the relationship between soil microbial community structures and environmental factors.Results and discussion
N fertilization significantly increased ammonia-oxidizing bacteria (AOB) abundance. Ammonia-oxidizing archaea (AOA) community structure was markedly changed in N fertilizer treatment and strongly affected by soil pH, while soil nitrate and water content correlated with AOB community structure. Soil nitrate was the key factor influencing nirK gene community structure, while soil pH and water content explained much of the variations of nosZ gene community. AOB-amoA and nosZ gene community diversities were influenced by precipitation regimes and interaction of N fertilization and precipitation regimes, respectively.Conclusions
N fertilization and precipitation regimes had significant influences on the changes of soil properties and microbial functional communities. Soil nitrification was mainly driven by AOB in the semi-arid grassland. Changes of substrate content and soil pH were the key factors in shifting functional microbial communities. The non-synergistic effects of N fertilization and precipitation regimes on the microbial functional groups indicated that the negative effect of lower pH induced by N fertilization would be alleviated by precipitation regimes, which should be well considered in grassland restoration.13.
为揭示连续定点不同氮用量对棉田土壤微生物的影响,于2018~2020年在阿拉尔连续3年定点设置6个氮处理:0 kg/hm2(N0)、90 kg/hm2(N1)、180 kg/hm2(N2)、270 kg/hm2(N3)、360 kg/hm2(N4)、450kg/hm2(N5),研究了连续3年定点施氮对0~60 cm土层三大菌群数量及结构比例的影响。结果表明,各处理0~60 cm土层土壤微生物均以细菌为主,放线菌次之,真菌最少。连续定点施氮可分别提高0~60 cm土层土壤细菌、真菌、放线菌总数,且随施氮量的增加基本呈先增后降的变化趋势。其中细菌、放线菌数量均在N3处理达到峰值,其数量变化均主要集中在0~40 cm土层(0~40 cm细菌占其总数的71.92%~79.65%,0~40 cm放线菌占其总数的77.21%~92.06%);真菌数量在N4处理达到峰值,其数量变化主要集中在0~20 cm土层(0~20 cm真菌占其总数的35.62%~65.84%)。随... 相似文献
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Jacob T. Bushong Eric C. Miller Jeremiah L. Mullock D. Brian Arnall William R. Raun 《Journal of plant nutrition》2016,39(13):1874-1890
With the demand for maize increasing, production has spread into more water limited regions. Couple this with increasing resource costs and environmental concerns and the need for efficient nutrient and water management practices has increased. The objective of this trial was to evaluate the effects of different nitrogen (N) fertilizer application methods and timings on maize grain yield, N use efficiency (NUE), and water use efficiency (WUE) under irrigated and rain-fed conditions. Four site-years of data were collected. Fertilizer treatments consisted of all N applied preplant, split surface applied, and split foliarly applied. Irrigation applied prior to and during reproductive growth increased grain yield, NUE, and WUE compared to rain-fed treatments for all site-years. Split surface applied N fertilizer applications typically increased NUE, but not always grain yield compared to preplant applications. The use of split foliar N fertilizer applications was only beneficial in the site-years when leaf burn was not as severe. 相似文献
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长期定位试验研究不同施肥措施对旱地玉米土壤(NO3--N)累积的影响结果表明,不同施肥和秸秆还田措施可不同程度造成0~500cm土层NO3--N的累积,且对0~300cm土层NO3--N的累积影响较大。秋施肥秸秆覆盖还田处理产量最高,且土壤NO3--N累积量较低,所造成的环境风险也小,为我国北方半湿润偏旱区适宜施肥措施。 相似文献
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石炭性土壤地区小麦氮肥施用技术研究 总被引:1,自引:0,他引:1
研究结果表明,本试验条件下,施N量<300kg@N/hm2时,N肥对小麦株高、1~5节间长度、上部叶片长度、分蘖、穗数、粒数、产量有显著的正效应,对粒重效应不明显;施N量>300kg@N/hm2时,N肥对株高、1~5节间长度、穗数、粒数的效应不明显,对倒3、4、5叶片长度仍有一定的正效应,对粒重和产量有显著的负效应.适当扩大中后期施肥比例,对株高、后期叶片、粒数、粒重和成穗率有正效应,对前期叶片、分蘖和穗数有一定负效应;扩大前期施肥比例,对前期叶片、分蘖、穗数有正效应,对株高的效应不明显,对成穗率、粒数、粒重有负效应.最佳产量结构和最高产量时的施N量为254.0kg@N/hm2,分配方式为基肥苗肥腊肥拔节孕穗肥粒肥等于32032 或4202.51.5. 相似文献
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研究结果表明,本试验条件下,施N量<300kg·N/hm2时,N肥对小麦株高、1~5节间长度、上部叶片长度、分蘖、穗数、粒数、产量有显著的正效应,对粒重效应不明显;施N量>300kg·N/hm2时,N肥对株高、1~5节间长度、穗数、粒数的效应不明显,对倒3、4、5叶片长度仍有一定的正效应,对粒重和产量有显著的负效应。适当扩大中后期施肥比例,对株高、后期叶片、粒数、粒重和成穗率有正效应,对前期叶片、分蘖和穗数有一定负效应;扩大前期施肥比例,对前期叶片、分蘖、穗数有正效应,对株高的效应不明显,对成穗率、粒数、粒重有负效应。最佳产量结构和最高产量时的施N量为254.0kg·N/hm2,分配方式为基肥:苗肥:腊肥:拔节孕穗肥:粒肥等于3:2:0:3:2 或4:2:0:2.5:1.5。 相似文献
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中国稻田施氮技术研究进展 总被引:5,自引:1,他引:5
提高N肥利用率,加强稻田养分管理已经成为水稻生产中研究的热点。针对我国稻田N肥利用率低和N素损失严重的现状,综述了国内外提高稻田N肥利用率技术途径的研究进展,包括改进施肥方法(确定适宜施N量、N肥深施、前N后移、平衡施肥、有机与无机肥配施、水肥综合管理)、新型肥料的研制(硝化抑制剂、脲酶抑制剂、表面分子膜、缓控释肥)、计算机决策支持系统指导施肥和实时、实地N肥管理等。展望了今后需要加强研究的几方面工作。 相似文献
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Straw return can be used to reduce fertilizer input and improve agricultural sustainability and soil health. However, how straw return and reduced fertilizer application affect beneficial soil microbes, particularly arbuscular mycorrhizal fungi (AMF), remains poorly understood. Here, we conducted a five-year field experiment in a rainfed maize field on the Loess Plateau of northwestern China. We tested four treatments with straw return combined with four nitrogen (N) application rates, i.e., 100%, 80%, 60%, and 0% of the common N application rate (225 kg N ha-1 year-1) in this region, and two reference treatments (full or no N application), with three replicates for each treatment. Mycorrhizal colonization was quantified and AMF communities colonizing maize roots were characterized using Illumina sequencing. Forty virtual taxa (VTs) of AMF were identified in root samples, among which VT113 (related to Rhizophagus fasciculatus) and VT156 (related to Dominikia gansuensis) were the predominant taxa. Both root length colonization and AMF VT richness were sensitive to N fertilization, but not to straw return; furthermore, both gradually increased with decreasing N application rate. The VT composition of the AMF community was also affected by N fertilization, but not by straw return, and the community variation could be well explained by soil available N and phosphorus concentrations. Additionally, 60%, 80%, and full N fertilization produced similar maize yields. Thus, our study revealed the response patterns of AMF to straw return and N fertilizer reduction and showed that straw return combined with N fertilizer reduction may be a promising practice to maintain mycorrhizal symbiosis concomitantly with crop productivity. 相似文献