Study on Cytoplasmic polyhedrosis virus on Dendrolimus superans

Cytoplasmicpolyhedrosisvirus(CPV)ofEendrolimussuperans(DsCPV)isone.oftheimportantpatholo-gensofthelarvaeOfDendFOHffiussuPerans.InoFdertoutiIizeDsCPVincontrol,themorphoIogy,toxicity,infeCtionanddestroyingprocedureinthemidgutofinsectofDsCPVwereStudied.ThecontrolofDen-dIOhffiussuPeranswiththisvirusinasmaIIareainforestwascarriedout.ResearchmethodMorphologyobservationScanningeIectronmicroscopeandtransmissioneIeCtronmicroscopewereusedtoobservethemor-phoIogy.ToxicityteStingThesameamo…     

Study on histopathology of nuclear polyhedrosis virus ofZethenia rufescentaria Motsch

This paper reports the histological observation of larvae ofZethenia rufescentaria Motsch. after infection by ZrNPV. Histopathologic study revealed that ZrNPV were multiplied within the nuclear of fat body, epidermis cell, midgut cell, tracheal matrix and blood cell. These cells showed obvious cytopathic effects. The nucleus of infected cells underwent swelled. Under electron microscope, virus and polyhedral of ZrNPV were clearly observed in these nucleus of infected cells. The nucleus of susceptible tissues were fulfilled with polyhedra after 70–140 h. This project was supported by the Natural Sciences Foundation of Heilongjiang Province Responsible Editor: Chai Ruihai     

New cell line establishment of a cell line from embryos ofDendrolimus superans

A new cell line was established from 5-day-old embryonated eggs ofDendrolimus superans and has been designated NEAU-Ds-950821. The cell line consists of mixture of cell types, including majority of spherical shaped cells and a few of spindle shaped cells. The cell line has a population of doubling time of 52.6 h. Chromosome analysis revealed typical lepidopteran chromosomes. Isozyme characterization of Esterase showed the patterns were different from other three cell lines (Ms-927311, Xc-920730, and SF-21AE). Virus infectivity tests revealed the cell line can supportD. superans cytoplasmic polyhedrosis virus. The project is supported by National Science Foundation of China Responsible editor: Chai Ruihai     

The influencen of stand state on the occurrence ofDendrolimus superans (Butler) in Daxing’an Mountains

Dendrolimus superans (Butler) occurred in the vast areas of Daxing’an Mountains forest, in Heilongjiang Province, in 1990. Stands damaged byI). Superans seriously, intermediately and lightly show patch shape distribution within damaged area. By ordination analysis and correlation analysis between ordination factors and coordinate axis. It was found that occurrence ofD Superans in different extent is owing to the effect of forest age, composition and exposure. The tended young pure forests grown on the sunward slopes are most seriously damaged and non-tended middle-age mixing stands grown on the sunless slopes are most lightly damaged. Therefore, in order to preventD. superans from seriously damaging larch forests, relatively large crown density (P > 0. 7) should be kept, and broadleaf trees should be also properly preserved. This research project was supported by Scientific and Techndogical Committee of Heilongjiang Province     

DCPV-Bt混合剂持续控制马尾松毛虫灾害的研究

利用松毛虫质型多角体病毒 (DCPV)与苏云金芽孢杆菌 (BacillusthuringiensisBerlner)混合防治松毛虫试验结果表明 :当 1ml混合剂含量为 0 2亿Bt芽孢 + 0 0 0 0 8～ 0 0 0 2亿CPB时 ,在 2 7℃条件下 ,松毛虫在喷施后 4～ 6d死亡率达70 % ,残存种群病毒感染率达到 5 0 % ,有效地控制了松毛虫的种群数量。通过酶联免疫技术 ,检测施用DCPV后林间松毛虫感染病毒情况 ,其中喷施DCPV长达 11a之久的松林 ,松毛虫带病毒率高达 78 5 % ,而且林中松毛虫种群密度一直保持较低水平     

Electroantennogram responses of Dendrolimus superans (Lepidoptera: Lasiocampidae) to six volatiles of Larix gmelinii

Dendrolimus superans is one of the important pests feeding on the needles of Larix gmelinii. Six standard compounds, (1R)-(−)-α-pinene, (1S)-(−)-α-pinene, ocimene, (1S)-(−)-β-pinene, R(−)-α-phellandrene and camphene, the main volatiles of L. gmelinii were used to test the electroantennogram (EAG) responses of moths. The results show that the order of the EAG responses of moths to seven concentrations of these six compounds were as follows: mated females > virgin females > unmated males, except for 0.1 μL/μL R(−)-α-phellandrene, where the order was: virgin females > mated females > unmated males. There are statistically significant differences between the EAG responses of virgin females and unmated males (p < 0.05), and also between mated females with virgin females and mated females and unmated males (p < 0.01), which suggests that the mated females are more sensitive to the volatiles of host plant. The results of EAG responses of the mated females, virgin females and unmated males indicate that they are more sensitive to R(−)-α-phellandrene than to the other volatile components. The active time for the EAG responses of both mated females and unmated males occurs during the night, i.e., from 20:00 to 04:00 hours. This is consistent with their eclosion, mating and oviposition periods. __________ Translated from Scientia Silvae Sinicae, 2007, 43(7): 55–60 [译自:林业科学]     

The effect ofDendrolimus superans breakout on growth of trees

The effect ofDendrolimus superans on growth of trees was investigated after the breakout in Shibazhan forest area in 1990. Stem analysis was conducted for determining the tree increment loss. The result showed that the current annual increment for extreme heavily damaged, heavily damaged, moderately damaged and lightly damaged stands is 14%, 39.8%, 64% and 82% of that of the control forest respectively. (Responsible Editor: Chai Ruihai)     

Study on histopathology of nuclear polyhedrosis virus ofZethenia rufescentaria Motsch

IntroductionZOtheni8ruf8scent8rtaMotsch.isoneofthemainpestsinlarchpIantationinNortheastChina.Inrecentyears,thepestcauseddisasterintheforestareaofHeilongjiangProvince.TheneedlesofIarchinsomeareawerebeeneatenupcompIetelyandthegrowthoflarchpIantationwasseriousIyaffected.Thenu-clearpoIyhedrosisvirusofZruf8scent8rinMotsch(ZrNPV)wasobtainedfromtheIarvaeofZruf6s-cent8finMotsch,in1991.ZrNPVwasmainpathogenOftheIarvaeofZrufescentBfisMotsch.TOxicitymeasuringindoorsandinvestigationinfieldprov…     

Population characteristics ofDendrolims superans in Daxing’an Mountains

Dendrolims superans produced one generation every two years. Large larvae and small larvae exist at the same time. It broke out in Daxiang an Mountains in 1990, and seriously destroyed growth ofLarix gmelini Rupr. In order to controlD superans, the population properties ofD superans were studied from 1991 to 1992, including sexratio, age distribution, pattern etc.. The sex ratio ofD. superans population is ♀∶♂ − 1.06:L. The larvae age distribution indicates that larvae over 5 instar is less than younger larvae under 4 instar in Yongqing forest farm, but the younger larvae under 4 instar is more than larvae over 5 instar in Hanjiayuanzi Forest Farm. The population ofD. superans in Yongqing Farm has been declining. and mereasing in Hanjiayuanzi. Pupae and eggs are mainly distributed in middle and lower crowns layer of trees respeetively account for 66% and 59.5% of total individuals. The pupae in higher crown layer is lightly regular distribution and clumping distribution middle and lower crowns layer of trees. The eggs in higher crown is light clumping distribution and random in middle and low crown of tree. (Responsible Editor: Zhu Hong)     

利用M-18型飞机喷洒高效生物药剂防治落叶松毛虫的研究

2004年6月,吉林省朝鲜族自治州应用M-18型飞机对落叶松毛虫进行了大面积的飞机生物防治研究。药剂选用阿维菌素,防治面积为0 4×104hm2,对落叶松毛虫的防治效果较好,其死亡率在95%以上。喷洒质量测定结果:药剂回收率为76 82%。     

Development, manufacture and application of Beta-Cypermethrin aqueous capsule suspension

The optimal formula of Beta-Cypermethrin aqueous capsule suspension was screened out through the experiment,preparation and manufacture of basic formulas, which has no sedimentation, suspended drops of oil, peels and agglomerating and the diameter of capsule is in range of 10-30μm, conformed to the technical requirement. The aftereffect of the 3.3% Beta-Cypermethrin aqueous capsule suspension which was manufactured according to the optimal formula was up more than 25 days. The experiments on controlling the larvae of Dendrolinmus superans Butler and the adults of Xylotrechus rusticus L were carried out with different concentrations of this chemical. The death rate reached 80% when 250 times solution of the chemical was sprayed on stem to control the larvae of D. superans. For control of the adults of X. rusticus, 200, 400 and 600 times solution of the chemical were applied and their control effects (death rate) reached 85.23%, 74.21% and 66.59% respectively. Two kinds of solution (200 times and 300 times) of the chemical were used to control the larvae of D. superans in large area, and the control effect for both concentrations was over 90%.     

马尾松毛虫质型多角体病毒(D.CPV)的研究Ⅱ.D.CPV加辅助剂的药效测定及贮藏方法探讨

对马尾松毛虫质型多角体病毒（Ｄ．ＣＰＶ）添加辅助剂的增效。试验表明：在Ｄ．ＣＰｖ中分别加入１％活性炭、２０％轻质碳酸钙、０．２％墨汁水三种辅助剂，在林间试验校正死亡率达８０．６％以上，比单纯病毒防治松毛虫效果好。Ｄ．ＣＰＶ浓度为２×１０￣６ＣＰＢ／ｍｌ加５０％甘油贮裁（冰箱２一４℃）１４个月，仍有较强的活性，致毒校正死亡率达７３％，比未加甘油的病毒冰箱贮藏、常温贮藏分别增效８％、２９．２％。     

马尾松毛虫质型多角体病毒单克隆抗体的制备和应用

纯化的马尾松毛虫质型多角体病毒（ＤｐＣＰＶ）云南文山株的病毒粒子经ＳＤＳＰＡＧＥ分析,其结构蛋白有１２０ｋｄ、１１６ｋｄ、１１０ｋｄ、６６ｋｄ和３３ｋｄ５个组分,而它的多角体蛋白含３０ｋｄ和２８ｋｄ两个主要组分。以纯化的ＤｐＣＰＶ粒子为抗原制备了２Ｄ５、２Ｄ１０、３Ｄ４和６Ｂ３共４种单克隆抗体,并测定了它们的亚类。制得的单克隆抗体用于ＤｐＣＰＶ的ＥＬＩＳＡ检测。对ＤｐＣＰＶ在棉铃虫卵巢细胞系ＳＦＥＨＡ８２１２和ＳＦＥＨＡ８３１中增殖动态的检测结果表明,ＤｐＣＰＶ感染培养细胞具有释放病毒量小和呈现持续感染等特点。用Ｗｅｓｔｅｒｎ印迹法在ＳＦＥＨＡ８３１细胞感染ＤｐＣＰＶ后第１８ｈ检测到病毒抗原的合成。运用所建立的免疫学检测方法,对几批采自ＤｐＣＰＶ防治林区的幼虫样品进行分析,结果表明,该方法适用于对ＤｐＣＰＶ的防治效果及其自然流行进行长时期、大规模的监测。     

20%灭·阿可湿性粉剂防治落叶松毛虫试验

试验于 2 0 0 1年 8～ 9月 ,在内蒙古赤峰市克什克腾旗桦木沟林场华北落叶松人工林内进行。结果表明 ,使用 2 0 %灭·阿可湿性粉剂进行地面常规喷雾和喷粉防治落叶松毛虫的效果显著 ,采用用药量 4 5 0～ 75 0g/hm2 进行常规喷雾防治 ,落叶松毛虫虫口减退率达 97%以上 ;采用药量 6 0 0～ 90 0g/hm2 进行常规喷粉 ,虫口减退率达 90 %以上 ,而且常规喷雾的防效好于常规喷粉     

德昌松毛虫质型多角体病毒防治大柏毛虫应用研究

应用德昌松毛虫质型多角体病毒ＤｅｎｄｒｏｌｉｍｕｓｐｕｎｃｔａｔｕｓｔｅｃｈａｎｇｅｎｓｉｓＣｙｔｏ－ｐｌａｓｍｉｃＰｏｌｙｈｅｄｒｏｓｉｓＶｉｒｕｓ（ＤｐｔＣＰＶ）防治四川内地柏木林区大柏毛虫Ｄ．ｈｏｕｉＬａｊｏｎ－ｑｕｉｅｒｅ的试验表明，以７．５×１０９ＰＩＢ／６６６．６ｍ２以上剂量防治３龄幼虫，其综合防效达８５％以上，且具有显著的扩散流行和持续防治效果。     

A PRELIMINARY STUDY ON THE NUCLEAR POLYHEDROSIS VIRUS OF LARCH CASEBEARER

The nucleopolyhedrosis virust(NPV)of larch casebearer(Dendrolimus superans)is inquadrangle or irregular form. It’s diameter is ranged from 1.21um to 2.33um.There is one ormany vrions in each envelope.The size of the virion is about 29.1--60.5nm×244.2--325.6nm     

僧夜蛾上一株新的核型多角体病毒

僧夜蛾上一株新的核型多角体病毒吴燕王贵成杨珍张卓然马耀关键词僧夜蛾、核型多角体、病毒僧夜蛾（ＬｅｉｏｍｅｔｏｐｏｎｓｉｍｙｒｉｄｅｓＳｔａｕｄｉｎｇｅｒ）又称白刺夜蛾。分布于我国内蒙、甘肃、宁夏、新疆等半干旱地区。主要危害白刺（Ｎｉｔｒａｒｉａｓｐｐ...     

Chrysolina fastuosa (Coleoptera: Chrysomelidae) a biological control agent or a possible pest?

A poorly known chrysomelid beetle, Chrysolina fastuosa (Scopoli) has been found in a strikingly great number on black horehound (Ballota nigra L.), a soft caulescent plant belonging to the Lamiaceae family. B. nigra can be found from April to August commonly on roadsides, along walls or at the border of gardens and orchards in Hungary and continental Europe. It can be called a minor weed. The glistering tiny adults that feed voraciously on leaves cause apparently significant damage. The subsequent investigation, which focuses on the number of feeding individuals and the loss of plant tissue, showed 1–24 beetles on a plant and the consumed leave surface which amounted to 8–94%. The repeated damage assessment, a week later showed a significantly unimportant increase in the number of individuals but a significant 20–31% increase in the plant surface loss. According to the literature, Ch. fastuosa has been found in association with other Labiatae plants such as Galeopsis pubescens Besser, Lamium album L. and Urtica dioica L. (Urticaceae). Thus, considering its efficiency mentioned above, it could be used to augment and maintain its populations as a biological control agent for these weeds. However, taking into consideration the growing importance of the production of medicinal plants among which Lamiaceae species are numerous, Ch. fastuosa can cause damage that cannot be neglected.     

Small hive beetle diagnosis and control in naturally infested honeybee colonies using bottom board traps and CheckMite + strips

The efficacy of bottom board traps and CheckMite + strips using Coumaphos, for small hive beetle (SHB) diagnosis and control was studied in Australia. Colonies in three apiaries (N = 10 each) were surveyed for SHB. In two apiaries, colonies received bottom board traps (cardboard or plastic) with CheckMite + strips and in the control apiary, traps without strips. After 5 days, all colonies were surveyed again, killed and dissected to quantify non-detected SHB. Significant differences in the number of live SHB were found between apiaries after treatment, supporting the fact that Coumaphos traps are efficient (trap mortality: 94.73 ± 0.06% cardboard and 99.53 ± 0.01% plastic). However, mortality assessment at the colony level (53.29 ± 31.30%), showed that only a limited SHB proportion was affected. Post mortem colony inspections revealed that 14.06 ± 10.53% SHB were undetected, which should be considered for quantitative diagnosis. Bottom board traps provided a first estimate of infestation levels (43.03 ± 27.02%).     

舞毒蛾核型多角体病毒室内增殖的研究

试验研究了饲毒龄期、饲毒浓度对舞毒蛾存活、幼虫体质量、虫尸质量和含毒量的影响以及饲毒时间对舞毒蛾含毒量的影响.结果表明:饲毒龄期和饲毒浓度对幼虫存活率和体质量增长均有不同程度的影响,饲毒龄期越小、饲毒浓度越高,死亡时间越早,死亡率越高,体质量增长越缓慢;而且饲毒时龄期越小、饲毒浓度越高,虫尸质量也越小,其含毒量也越低.饲毒时间与含毒量成抛物线型,饲毒时间过短或过长,体内含毒量均较低.由于5龄幼虫饲毒后有78％以上个体发育至蛹期,不发生死亡现象,因此,室内增殖舞毒蛾核型多角体病毒时,应选取4龄初幼虫喂饲1.0×106 PIB·mL-1病毒为宜,接毒10d后开始收集饲毒幼虫.