Investigation of amber durum wheat for production of yellow alkaline noodles

Samples of Canadian amber durum wheat varieties, of various protein content and a composite of export cargo samples, were milled to yield straight-grade and patent flours by reducing semolina and processed into yellow alkaline noodles (YAN). Samples of Canada Hard White Spring (CWHWS) and Canada Western Red Spring (CWRS) were included for comparative purposes. YAN from durum wheat displayed a colour advantage over CWRS and CWHWS YAN. The durum YAN displayed an approximate 9–20 unit greater b* (yellowness) value than CWRS and CWHWS at 2 and 24 h after preparation. This relates to greater yellow pigment and flavonoid contents in the durum flours. All durum wheat YAN exhibited excellent noodle brightness, which was retained over time due to lower levels of the enzymes polyphenol oxidase (PPO) and peroxidase (POD). Durum noodles displayed significantly fewer specks than CWRS and were comparable to CWHWS. Durum wheat YAN cooking quality was equal to or slightly superior to CWRS and CWHWS. Durum wheat flour refinement imparted no significant effects on cooked noodle texture (maximum cutting stress, recovery, resistance to compression). However, the various texture parameters improved with durum wheat protein content and gluten strength.     

Quality and antioxidant properties of instant noodles enhanced with common buckwheat flour

The objective of this study was to investigate the feasibility of incorporating common buckwheat (Fagopyrum esculentum Moench) into instant noodle formulations. Australian Soft (AS) and Baker's flours were used to evaluate the effects of varying buckwheat contents (0–40%) on noodle quality. The results of texture analysis indicate that noodles made using AS flour produced softer texture whereas there was minimal effect for Baker's flour when buckwheat was incorporated. The colour, measured by L* values, decreased with increased addition of buckwheat for both flours. Fat uptake for noodle samples made from AS flour was only marginally affected, but increased for Baker's flour, when higher levels of buckwheat flour were added. The antioxidant rutin was detected in noodles made from both wheat flours, generally increasing with % buckwheat flour added. These findings indicate that the incorporation of 20% buckwheat into the formulation can be used to enhance the quality of instant noodles.     

White salted noodle characteristics from transgenic isolines of wheat over expressing puroindolines

The closely linked genes puroindoline a (Pina) and puroindoline b (Pinb) control most of the variation in wheat (Triticum aestivum) grain texture. Mutations in either Pina or Pinb result in hard grain with wild type forms of both genes giving soft grain. Asian noodles are prepared from both hard and soft classes of wheat. Our objective was to examine color and texture characteristics of white salted noodles processed from flours of transgenic isolines of Hi-Line hard red spring wheat over expressing Pina-D1a, Pinb-D1a or both and a control giving a range in grain texture from very soft to hard. White salted noodles were prepared and color and texture characteristics were measured. The three softer textured transgenic isolines showed greater change in L^* with time than Hi-Line. The noodles were more adhesive (more negative value), firmer, and chewier as the grain texture became successively softer when cooked at 5 min. These texture differences were not as apparent when noodles were cooked for an optimum time. Starch pasting properties did not explain the noodle textural differences. A possible explanation for the noodle texture differences may be related to starch damage which ranged from 2.2% for HGAB to 6.7% for Hi-Line, flour particle size differences and subsequent water absorption differences among the four genotypes. Over expression of puroindolines did not enhance quality of white salted noodles when prepared under these conditions.     

小麦淀粉与面条质量关系的研究进展

小麦淀粉品质对白盐面条的质量（尤其是煮后的感官特性）有重要影响。直链与支链淀粉的含量及比例是影响面条质量的重要因素,是造成不同小麦品种淀粉糊化和膨胀特性及面条质量差异的物质基础。较低直链淀粉含量的小麦粉具有较好的糊化和膨胀特性,制作的面条煮制时吸水率高,烹调损失低,具有较高的感官评分。优质白盐面条的直链淀粉含量应在22%左右。峰值黏度、稀懈值、峰值时间是影响面条质量的重要糊化参数,这3项参数高的小麦粉适合制作优质面条。高膨胀势或膨胀体积的小麦粉制作的面条中等偏软,光滑且富有弹性,可以作为面条用小麦的重要选择标准。一般认为,直链淀粉含量较低、峰值黏度和稀懈值高、峰值时间长、膨胀势或膨胀体积高的小麦粉适合制作优质白盐面条。其中,直链淀粉含量、峰值黏度和膨胀势是优质面条小麦评价的关键品质性状。     

Effect of A- and B-granules of wheat starch on Chinese noodle quality

To investigate the effect of A- and B-type granules of wheat starch on noodle quality, the fractionation and reconstitution method was used to prepare noodles with five different ratios of A-to B-granules (100A-0B, 75A-25B, 50A-50B, 25A-75B, 0A-100B). The pasting and swelling properties of reconstituted flours and the microstructure of noodles observed under a confocal laser scanning microscope (CLSM) indicated that there were at least two aspects were responsible for the changes in noodle quality. First, the water distribution, texture and cooking quality of noodles were influenced by the different physicochemical properties of A- and B-granules; and second, the gluten structure in noodles was altered by the granule size distribution, which further led to a difference in noodle quality. In general, with increasing number of B-granules, the A₂₂ (the proportion of less immobilized water) of raw noodles and the hardness, resilience and chewiness of cooked noodles increased first and then decreased, while the cooking loss, water absorption and protein loss showed the reverse trend. Raw noodle samples containing 50A-50B had the minimum T₂₂ (less immobilized water) but the maximum A₂₂ and, when cooked, had the greatest hardness, chewiness and resilience and the least cooking loss (6.6%) and water absorption (166.1%). These results have important implications for illustrating the mechanism by which A- and B-granules affect noodle quality and guide efforts to improve noodle quality and wheat breeding.     

The antioxidant potential of milling fractions from breadwheat and durum

The effect of primary processing, namely milling, on the phenolic content and antioxidant capacity of two wheat cultivars, namely Canada Western Amber Durum (Triticum turgidum L. var. durum) and Canada Western Red Spring (Triticum aestivum L.) was studied. The milling products: bran, flour, shorts and feed flour fractions were examined. In addition, semolina was an end-product of durum wheat milling. Antioxidant activity of wheat phenoliocs was evaluated using oxygen radical absorbance capacity, inhibition of photochemiluminescence, the Rancimat method, and inhibition of oxidation of low-density lipoprotein cholesterol and deoxyribonucleic acid. The phenolic composition of wheat extracts was determined using high performance liquid chromatography. Bran showed the highest antioxidant activity whereas the endosperm possessed the lowest in both cultivars examined. The phenolic compounds are concentrated in the outermost layers namely the bran. The consumption of wheat with bran in the form of whole grain may provide beneficial health effects.     

Instrumental Texture of White Salted Noodles Prepared from Recombinant Inbred Lines of Wheat Differing in the Three Granule Bound Starch Synthase (Waxy) Genes

Texture is a key quality determinant of white salted noodles (WSN). Recombinant inbred lines (RILs) (n=130) of wheat with all combinations of the three Waxy genes provided a unique opportunity to relate granule bound starch synthase (GBSS) gene dosage with the texture of WSN using texture profile analysis. Harder noodles tended to be more adhesive and chewier (r=−0·68 and 0·97, respectively), whereas a reduction in adhesiveness was correlated with greater cohesiveness (r=0·65), springiness (r=0·72), and resilience (r=0·66). Cohesiveness and resilience were highly correlated (r=0·94). Highly significant variation existed among the RILs for all noodle texture parameters. Normal starch RIL noodles tended to produce the hardest, most adhesive and chewy, but least cohesive, springy and resilient noodles. Full waxy RIL noodles were the softest, thickest, least adhesive and chewy, and most cohesive and springy. Partial waxy RIL noodles generally were intermediate in texture. The GBSS genotype comprised 27–68% of the variance of noodle texture compared to 27–60% variance for RILs within GBSS class. Based on these results, starch composition is a major contributor to the texture of white salted noodles.     

Partial purification and characterization of polyphenoloxidase from durum wheat (Triticum durum L.)

A bright yellow color of pasta is an important qualitative trait for the durum wheat industry. Final color is the result of the balance between yellow and brown components in semolina. Polyphenoloxidase (PPO) is implicated as playing a significant role in darkening. This study aimed to characterize PPO activity of durum wheats. PPO was extracted and partially purified by ion-exchange chromatography on a column packed with diethyaminoethyl cellulose (DEAE). This procedure led to 26.33-fold purification with 24.7% recovery. The optimum temperature and pH of PPO were found to be 40 °C and 6.5, respectively. Heat stability of durum wheat PPO decreased as the temperatures increased from 30 to 80 °C. The z-value was calculated as 23.4 °C. It increased to 26.3 and 48.4 °C in the presence of 40% sucrose and 1 M NaCl, respectively. Durum wheat PPO was shown to use several phenolic compounds as substrate. Among the substrates used, the greatest substrate specificity was observed with catechol. Durum wheat PPO was sensitive to inhibitors such as ascorbic acid, cysteine, oxalic acid and citric acid. Ascorbic acid was the most effective inhibitor.     

Chemical structure of flavonoid compounds in wheat (Triticum aestivum L.) flour that contribute to the yellow colour of Asian alkaline noodles

Colour is an important criterion of quality in Asian noodles prepared from bread-wheat flour and ranges from white to cream for white salted noodles to a bright yellow for alkaline noodles. A high-performance liquid chromatographic protocol for the analysis of naturally occurring flavonoids and related phenolic compounds in wheat was developed to identify the compounds responsible for the yellow colour of alkaline noodles. The separation system consisted of a C18 reversed-phase column and a gradient system of water/formic acid (99:1,v/v) (A) and methanol/acetonitrile/formic acid (95:4:1,v/v/v) (B). A post column derivatisation technique was employed to identify the compounds that turned yellow at alkaline pH. A comparison of the HPLC profiles of water, alkaline water and hydroxylamine soluble fractions revealed that the level of compounds contributing to yellow alkaline noodle colour was highest in the hydroxylamine extract. Two flavonoid apigenin-C-diglycosides and their Wessely–Moser isomers, together with their sinapic acid esters, were isolated and identified by absorbance spectroscopy, ES-MS spectrometry and ¹³C NMR. These flavone-C-diglycosides were found to be the major components responsible for the alkali-induced change in noodle colour and are relatively stable at ambient temperature. Furthermore the compounds are located only in the germ (embryo+scutellum tissue) of wheat grains and hence their presence in flour must represent a redistribution during the milling process.     

Lowering carbon footprint of durum wheat by diversifying cropping systems

Improving cropping systems may help mitigate greenhouse gas emissions. This study determined the carbon footprint of durum wheat (Triticum turgidum L.) produced in diverse cropping systems. Durum was grown in rotation systems which had different combinations of oilseed, pulse, and cereal crops at five site-years in Saskatchewan, Canada. Total greenhouse gas emissions from the decomposition of crop residues along with various production inputs were used for the estimation of carbon footprint. On average, emissions from the decomposition of crop straw and roots accounted for 25% of the total emissions, those from the production, transportation, storage, and delivery of fertilizers and pesticides to farm gates and their applications 43%, and emissions from farming operations 32%. Durum wheat preceded by an oilseed crop (Brassica napus or Brassica juncea) the previous year had carbon footprint of 0.33 kg CO₂e kg⁻¹ of grain, or 7% lower than durum in cereal-cereal-durum system. Durum preceded by a biological N-fixing crop (Cicer arietinum chickpea, Lens culinaris lentil, or Pisum sativum pea) the previous year lowered its carbon footprint by 17% compared with durum preceded by a cereal crop. Durum produced in a pulse-pulse-durum system had carbon footprint 0.27 kg CO₂e kg⁻¹ of grain, 34% lower than durum grown in cereal-cereal-durum systems. Diversifying cropping systems with oilseeds and biological N-fixers significantly lowered carbon footprint of durum wheat.     

小麦蛋白质与面条品质关系的研究进展

为促进我国面条工业生产并为面条专用小麦良种的选育提供科学依据,对近年来小麦蛋白质与面条品质关系的研究进行了综述。总体来看,蛋白质含量和质量从各个方面影响面条品质。蛋白质含量与面条白度、表观状态呈负相关,与面条质构特性,尤其是煮熟面条的硬度、弹性呈正相关。较高的麦谷蛋白含量可以提高面条的硬度、咀嚼性、粘合性和抗拉伸能力。在一定范围内,蛋白质质量（面筋含量和质量、沉降值、流变学特性）的提高有助于面条蒸煮品质的改善和感官评分的提高,但蛋白质质量过高（湿面筋含量〉35%,沉降值〉60mL,稳定时间〉16min）会导致面条外观品质变劣,评分下降;高分子量谷蛋白亚基5＋10可能与较好的面条品质有关。一般认为,优质面条小麦的蛋白质品质指标为：蛋白质含量12%～14%;湿面筋含量28%～34%,沉淀值40～45mL,稳定时间5～15min。     

Physicochemical characterization and in-vitro digestibility of extruded rice noodles with different amylose contents based on rheological approaches

The physicochemical properties and in-vitro digestibility of extruded rice noodles with different amylose contents were characterized from a rheological point of view. Thermo-mechanical measurements showed that the rice flour with higher amylose contents exhibited greater stability to dual-mixing and higher degrees of starch gelatinization and retrogradation. In addition, greater elastic properties were clearly observed in the high amylose rice samples. The use of high amylose rice flour produced noodles with a harder texture, consequently contributing to reduced cooking loss. Furthermore, the rheological changes of extruded rice noodles were monitored in real time during the in-vitro starch digestion. The rice noodle digesta with higher amylose contents exhibited greater viscosities throughout the simulated oral-gastric-intestinal digestion steps. The flow behaviors of the rice noodle digesta consisted of the Power-law region and infinite shear plateau that were satisfactorily characterized by the Sisko model (R² > 0.99).     

Effects of potato/wheat flours ratio on mixing properties of dough and quality of noodles

The effects of potato flour/wheat flour (PF/WF) ratio on the mixing properties of dough and the quality of noodles were investigated. Substitution of wheat flour with potato flour caused the increases in the water absorption and the protein weakening value (C1-C2) of formulated flours, as well as the decrease in the corresponding dough mixing parameters including dough development time (DDT), dough stable time (DST), initial mixing time (C2), maximum torque during heating (C3), minimum torque of hot paste (C4) and the amount of retrogradation (C5-C4), indicating that the addition of PF weakened dough strength but improved degradation resistance. The textural, cooking, sensory characteristics and microstructure of noodle samples were evaluated. The results indicate that adhesiveness, springiness, broken ratio and cooking lose increased progressively with increasing PF content while the score of sensory evaluation continued to decrease. However, hardness, cooking yield and optimum cooked time for the samples of PF content range from 10% to 20% showed a distinctive variation. Environmental scanning electron microscopy (ESEM) confirmed changes in noodle microstructure as PF addition affected gluten network development. In general, noodles with PF content below 40% were acceptable.     

拉面制作过程和产品感官评价方法的评估

为给拉面质量评价、拉面用粉标准的制定提供感官评价方法,采用走访询问、现场调查和网络交流等方式,收集拉面师傅对实验室制定的拉面制作过程感官评价方法的意见和建议;以餐饮市场常用小麦粉为样品原料,在兰州市和北京市分别开展消费者对拉面产品感官评价要素重视程度的问卷调查,分析、评估和确定了拉面制作过程和产品感官评价方法。88.89%的拉面师傅认为,所制定的拉面制作过程和感官评价方法可以概括拉面制作的整个过程,其对评价指标的重视程度依次为"和面难易程度及面团软硬程度""断条扣数及均匀性""跐面力度及黏手程度""醒发后面团色泽"="拉伸力大小";制定的拉面制作工艺可使大多数小麦粉满足加工工艺要求。消费者对产品感官评价要素的重视程度由高到低为弹性色泽硬度光滑性表观黏性;消费者完全可以区分出拉面用小麦粉的质量优劣。本研究制定的方法可用于拉面制作过程和产品感官质量的评价。在百分制评价体系中,拉面制作过程感官评价赋值分别为"和面难易程度及面团软硬程度"35分、"断条扣数及均匀性"20分、"跐面力度及黏手程度"15分、"醒发后面团色泽"15分、"拉伸力大小"15分;拉面产品消费者感官评价赋值分别为"弹性"30分、"硬度"20分、"色泽"20分、"表观性状"10分、"光滑性"10分、"黏性"5分、"食味"5分。     

Use of peanut and cowpea in wheat-based products containing composite flours

Cowpeas and peanuts are legumes of major dietary and economic importance. They are favored worldwide because of their palatability, contribution to nutritional status, and low cost as a protein source compared to animal protein. Flours processed from cowpeas and peanuts have unique physico-chemical and sensory properties when used in composite flour mixtures. Appropriate blends of cowpea and peanut flours to replace wheat flour in Chinese-type noodles, muffins, and tortillas were determined using modelling and optimization procedures. For noodles, 15% peanut flour and 8% cowpea flour supplementation produced an acceptable product with high protein content (21%). For wheat flour replacement in muffins, up to 43% cowpea and up to 44% peanut flours may be used. However, when wheat flour replacement is 50% or greater, cassava flour should comprise 56 to 72% of the blend with a few exceptions. In tortillas, 24% cowpea and 46% peanut flours produced products similar in quality characteristics to those made from 100% wheat flour. The systematic approach used in these studies is more efficient than the traditional substitution method to optimize sensory qualities of wheat-based products containing composite flours.     

几个黑小麦品种(系)蛋白质品质特性及其与面条蒸煮品质的关系

为给黑小麦加工品质评价及相关加工企业的原料选择与质量控制提供参考依据,选用国内推广的10个黑小麦品种为原料,对其蛋白质品质、粉质参数、面条蒸煮品质及其关系进行了分析.结果表明,黑小麦的蛋白质含量、谷蛋白大聚体(GMP)、谷蛋白溶涨指数(SIG)及蛋白组分中的醇溶蛋白和谷蛋白含量均低于对照小偃6号;湿面筋含量、面筋指数、沉淀值及粉质参数均优于小偃6号.黑小麦的沉淀值、SIG值和GMP含量、评价值与面条蒸煮干物质失落率呈极显著或显著负相关,逐步回归分析表明,SIG值和GMP与面条蒸煮干物质失落率的关系最为密切,是黑小麦面条品质评价的有效预测指标.黑小麦76、黑宝石、黑小麦、ZL97(6)和贞选1号具有较好的面条加工适用性.     

关中平原小麦粉鲜面条制作适宜性研究

针对餐饮业连锁经营和向食品制造转化的背景下,食品生产企业对小麦品种质量特性、原料质量稳定性,特别是面条制作适宜性提出新的要求,对关中平原大面积种植的小麦品种籽粒质量性状进行分析,并通过训练的专业感官评价人员对以其为原料、由面点师实验室制作的鲜面条的烹饪特性进行评价,同时统计分析小麦籽粒性状、面粉理化特性和面条感官评价结果之间的关系。结果表明,关中平原小麦千粒重、容重和硬度较高,面粉湿面筋含量高,面团延展性能良好,优质强筋小麦品种的比例明显提高。面粉的黏度参数,特别是峰值黏度,对面条的感官评价总分,面条质量要素中的色泽、硬度和黏性有较大影响,是评价鲜面条专用粉的重要参考指标。面条感官评价总分与峰值黏度呈显著正相关,与籽粒硬度、破损淀粉含量和面粉吸水率呈显著或极显著负相关。小麦品种（面粉）百农207、F50、F20、中麦578、西农583和郑麦366适宜制作优质鲜面条。     

面条品质评价指标及评价方法的研究

为了全面科学地评价小麦品种的面条加工特性,测定了29个北方小麦品种的面粉品质和鲜湿面条的制作品质,采用相关分析和多元回归分析方法,分析了面粉品质指标与鲜面条加工品质之间的关系.结果表明,面条的蒸煮损失率、拉伸强度、拉断力、延伸率与面条综合得分间的相关系数分别为-0.429、0.944、0.796和0.398,相关性很强,可以用作面条品质的辅助评价指标,即辅助综合得分来评价面条品质;面团的形成时间、稳定时间及弱化度与面条的综合得分和蒸煮损失率均呈极显著相关,面粉中的总淀粉含量与面条的综合得分呈显著相关,并且面粉白度、蛋白质含量、湿面筋含量、沉淀值、吸水率、形成时间、稳定时间、弱化度、总淀粉含量、直链淀粉含量、支链淀粉含量、支直链淀粉比等12项指标都不同程度地进入了面条的上述5个品质评价指标的回归方程,进一步说明用这5个指标来综合评价面条品质是可取的.根据面条各品质指标的相关性推出各品质指标在面条综合评价中所占的权重,再将利用回归方程算出的5个面条品质评价指标的数据进行标准化处理以消除量纲的影响后,即可计算出某个小麦品种所生产面条的综合评价分数.本研究即通过这一方法从29个供试小麦品种中优选出了6个优质面条用小麦品种,它们分别是洛展1号、泰山23、温麦4号、太空6号、淮麦16、温麦8号.     

A Single Genetic Locus Associated with Starch Granule Properties and Noodle Quality in Wheat

An extensive survey of wheat cultivars showed a complete association between the presence of a granule bound starch synthase (GBSS-4A) null mutation and the classification of the respective cultivar into the white salted noodle category. Since flour swelling volume (FSV) is a standard assay of flour used to identify cultivars that have potential for the production of white salted noodles, the results of the survey were investigated further by studying the genetic association of GBSS-4A null genotypes and FSV. A study of 34, F2 derived families, from a cross between wheat cultivars Reeves (good noodle texture, high FSV) and Kulin (poor noodle texture, low FSV), provided clear evidence for the genetic association of a granule bound starch synthase (GBSS-4A) null mutation and FSV. The molecular basis for the affect of the GBSS-4A null mutation was not simply due to a decrease in amylose content in the starch granules. Instead, the null mutation most likely causes a subtle change in starch structure as indicated by the association of high starch viscosity with high FSV and the GBSS-4A null genotype. The study suggests that assaying for the GBSS-4A null mutation at the DNA level may provide a valuable screen for noodle quality at early stages in a breeding program aimed at good white salted noodle quality.     

Characterization of a gluten reference material: Wheat-contaminated oats

Oat could be a good addition to the Gluten Free diet, but the purity of the oat supply is under scrutiny. As celiac disease becomes more prevalent, better detection tools for gluten in oats are necessary. We aimed to produce reference materials (RMs) of Canada Western Red Spring wheat (CWRS)-contaminated oat flour. Pure, uncontaminated oats flour (cultivars Navan and Gehl) was supplied by Cream Hill Estates. CWRS samples were provided by the Canadian Grain Commission from the 2009 Harvest Sample program. RMs containing 0, 20 and 100 ppm CWRS gluten-contaminated oats were created using a V-shell blender and tested by sandwich-type ELISA for gluten. Marked variations in ELISA results for the RMs were found among different test kits due to differences in capture antibodies and kit construction. The Veratox test was accurate at the 0, 20 and 100 ppm levels but detected only 30% (Veratox) and 50% (Veratox R5) of gluten at the 1000 ppm level; the Ridascreen test was accurate at all levels; the Biokits test detected roughly 10% of the gluten dosage; the Gluten-Check test detected some 30% of the gluten dosage. The RMs created could serve as standards for gluten detection in oat containing foods.